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| 1 | Malate transported from chloroplast to mitochondrion triggers production of ROS and PCD in Arabidopsis thaliana显示文摘规划房间死亡(PCD ) 是一个基本生物过程。在马赛克死亡 1 的缺乏(MOD1 ) ,质体局部性的 enoyl-ACP reductase,导致反应的氧种类(ROS ) 和 PCD 的累积,它能被 mitochondrial 建筑群压制我变化,从叶绿体显示一个信号到线粒体。然而,这个信号尚待被阐明。在这研究,通过克隆并且分析一系列 mod1 suppressors,我们揭示调整 mitochondrial ROS 和扳机 PCD 的产生的一条全面细胞器通讯小径。我们证明在 PLASTIDIAL NAD 依赖的 MALATE 脱氢酶(plNAD-MDH ) 的变化, chloroplastic DICARBOXYLATE TRANSPORTER 1 (DiT1 ) 并且(mMDH1 ) MITOCHONDRIAL MALATE 脱氢酶 1 能各在 mod1 救 ROS 累积和 PCD 显型,经由 malate 梭从叶绿体表明直接通讯到线粒体。进一步的研究证明这些元素在不同光周期条件下面在氧化还原作用动态平衡和植物生长起关键作用。而且,我们表明 ROS 水平和 PCD 显著地在房间,它能是戏剧性地稀释了由的对待 malate 的 HeLa 被增加人的基因 MDH2 击倒, Arabidopsis mMDH1 的 ortholog。这些结果揭开在植物和动物系统的一条保存导致 malate 的 PCD 小径,革命化我们在细胞器之间的通讯的理解。 | Yannan Zhao Lilan Luo Jiesi Xu Peiyong Xin Hongyan Guo Jian Wu Lin Bai Guodong Wang Jinfang Chu Jianru Zuo Hong Yu Xun Huang Jiayang Li | 2018 | Cell Research2018，28，4： | 24 |
| 2 | Simple and cost-effective methods for precise analysis of trace element abundances in geological materials with ICP-MS显示文摘Inductively coupled plasma mass spectrometry(ICP-MS) is the most commonly used technique to determine the abundances of trace elements in a wide range of geological materials. However, incomplete sample digestion, isobaric interferences and instrumental drift remain obvious problems that must be overcome in order to obtain precise and accurate results. For this reason, we have done many experiments and developed a set of simple, cost-effective and practical methods widely applicable for precise and rapid determination of trace element abundances in geological materials using ICP-MS. Commonly used high-pressure digestion technique is indeed effective in decomposing refractory phases, but this inevitably produces fluoride complexes that create new problems. We demonstrate that the fluoride complexes formed during high-pressure digestion can be readily re-dissolved using high-pressure vessel at 190 °C for only 2 h for 50 mg sample. In the case of isobaric interferences, although oxide(e.g., MO+/M+) and hydroxide(e.g., MOH+/M+) productivity is variable between runs, the(MO+/M+)/(Ce O+/Ce+)and(MOH+/M+)/(Ce O+/Ce+) ratios remain constant, making isobaric interference correction for all other elements of interest straightforward, for which we provide an easy-to-use off-line procedure. We also show that mass-time-intensity drift curve is smooth as recognized previously, for which the correction can be readily done by analyzing a quality-control(QC) solution and using off-line Excel VBA procedure without internal standards. With these methods, we can produce data in reasonable agreement with recommended values of international rock reference standards with a relative error of <8% and precision generally better than 5%. Importantly, compared to the widely used analytical practice, we can effectively save >60% of time(e.g., <24 h vs. >60 h). | Shuo Chen Xiaohong Wang Yaoling Niu Pu Sun Meng Duan Yuanyuan Xiao Pengyuan Guo Hongmei Gong Guodong Wang Qiqi Xue | 2017 | Science Bulletin2017，62，4： | 17 |
| 3 | Ginsenoside Rb1 attenuates activated microglia-induced neuronal damage显示文摘The microglia-mediated inflammatory reaction promotes neuronal damage under cerebral ischemia/hypoxia conditions. We therefore speculated that inhibition of hypoxia-induced microglial activation may alleviate neuronal damage. To test this hypothesis, we co-cultured ginsenoside Rb1, an active component of ginseng, and cortical neurons. Ginsenoside Rb1 protected neuronal morphology and structure in a single hypoxic culture system and in a hypoxic co-culture system with microglia, and reduced neuronal apoptosis and caspase-3 production. The protective effect was observable prior to placing in co-culture. Additionally, ginsenoside Rb1 inhibited levels of tumor necrosis factor-α in a co-culture system containing activated N9 microglial cells. Ginsenoside Rb1 also significantly decreased nitric oxide and superoxide production induced by N9 microglia. Our findings indicate that ginsenoside Rb1 attenuates damage to cerebral cortex neurons by downregulation of nitric oxide, superoxide, and tumor necrosis factor-α expression in hypoxia-activated microglia. | Lining Ke Wei Guo Jianwen Xu Guodong Zhang Wei Wang Wenhua Huang | 2014 | Neural Regeneration Research2014，9，3： | 11 |
| 4 | Ride comfort evaluation for road vehicle based on rigid-flexible coupling multibody dynamics显示文摘In the present research two different whole vehicle multibody models are established respectively, including rigid and rigid-flexible coupling multibody vehicle models. The former is all composed by rigid bodies while in the later model, the flexible rear suspension is built based on the finite element method (FEM) and mode superposition method, in which the deformations of the components are considered. The ride simulations with different speeds are carried out on a 3D digitalized road, and the weighted root mean square (RMS) of accelerations on the seat surface, backrest and at the feet are calculated. The comparison between the responses of the rigid and rigid-flexible coupling multibody models shows that the flexibility of the vehicle parts significantly affects the accelerations at each position, and it is necessary to take the flexibility effects intoaccount for the assessment of ride comfort. | Guangqiang Wu Guodong Fan Jianbo Guo | 2013 | Theoretical & Applied Mechanics Letters2013，3，1： | 11 |
| 5 | First dark matter search results from the PandaX-Ⅰ experiment显示文摘We report on the first dark-matter(DM)search results from PandaX-I,a low threshold dual-phase xenon experiment operating at the China JinPing Underground Laboratory.In the 37-kg liquid xenon target with 17.4 live-days of exposure,no DM particle candidate event was found.This result sets a stringent limit for low-mass DM particles and disfavors the interpretation of previously-reported positive experimental results.The minimum upper limit,3.7×10-44cm2,for the spin-independent isoscalar DM-particle-nucleon scattering cross section is obtained at a DM-particle mass of 49 GeV/c2at 90%confidence level. | XIAO MengJiao XIAO Xiang ZHAO Li CAO XiGuang CHEN Xun CHEN YunHua CUI XiangYi FANG DeQing FU ChangBo GIBONI Karl L. GONG HaoWei GUO GuoDong HU Jie HUANG XingTao JI XiangDong JU YongLin LEI SiAo LI ShaoLi LIN Qing LIU HuaXuan LIU JiangLai LIU Xiang LORENZON Wolfgang MA YuGang MAO YaJun NI KaiXuan PUSHKIN Kirill REN XiangXiang SCHUBNELL Michael SHEN ManBing STEPHENSON Scott TAN AnDi TARL Greg WANG HongWei WANG JiMin WANG Meng WANG XuMing WANG Zhou WEI YueHuan WU ShiYong XIE PengWei YOU YingHui ZENG XiongHui ZHANG Hua ZHANG Tao ZHU ZhongHua | 2014 | Science China(Physics,Mechanics & Astronomy)2014，57，11： | 10 |
| 6 | PandaX: a liquid xenon dark matter experiment at CJPL显示文摘PandaX is a large liquid-xenon detector experiment usable for direct dark-matter detection and 136Xe double-beta decay search.The central vessel was designed to accommodate a staged target volume increase from initially 120 kg(stage I)to 0.5 t(stage II)and eventually to a multi-ton scale.The experiment is located in the Jinping Deep-Underground Laboratory in Sichuan,China.The detector operates in dual-phase mode,allowing detection of both prompt scintillation,and ionization charge through proportional scintillation.In this paper a detailed description of the stage I detector design and performance as well as results established during the commissioning phase are presented. | CAO XiGuang CHEN Xun CHEN YunHua CUI XiangYi FANG DeQing FU ChangBo GIBONI Karl L. GONG HaoWei GUO GuoDong HE Ming HU Jie HUANG XingTao JI XiangDong JU YongLin LI ShaoLi LIN Qing LIU HuaXuan LIU JiangLai LIU Xiang LORENZON Wolfgang MA YuGang MAO YaJun NI KaiXuan PUSHKIN Kirill REN XiangXiang SCHUBNELL Michael SHEN ManBing SHI YuJie STEPHENSON Scott TAN AnDi TARLé Greg WANG HongWei WANG JiMing WANG Meng WANG XuMing WANG Zhou WEI YueHuan WU ShiYong XIAO MengJiao XIAO Xiang XIE PengWei YE Tao YOU YingHui ZEN XiongHui ZHANG Hua ZHANG Tao ZHAO HaiYing ZHAO Li ZHOU XiaoPeng ZHU ZhongHua | 2014 | Science China(Physics,Mechanics & Astronomy)2014，57，8： | 9 |
| 7 | Hydrothermal Synthesis of Molybdenum Disulfide for Lithium Ion Battery Applications显示文摘Molybdenum disulfide nanoflakes were synthesized by a simple hydrothermal process using sodium molybdate and thiourea as reactants at a relatively low temperature. X-ray diffraction(XRD) and transmission elec-tron microscopy(TEM) indicate that the samples have the structure of 2H-MoS2 and the morphology of nanoflakes with the average thickness around 5-10 nm. The results of electrochemical properties indicate that the morphology and size of MoS2 particles have effects on their capacity when they are used as the anode for lithium ion battery. The as-prepared MoS2 samples have high reversible discharge capacity up to 994.6 mA·h·g-1 for the MoS2-1 elec-trode and 930.1 mA·h·g-1 for the MoS2-2 electrode and show excellent cycling performances. The MoS2-1 electrode has a better cycling stability than the MoS2-2 electrode due to their difference in the uniformity of the samples. | WANG Shiquan LI Guohua DU Guodong JIANG Xueya FENG Chuanqi GUO Zaiping KIM Seung-Joo | 2010 | Chinese Journal of Chemical Engineering2010，18，6： | 9 |
| 8 | Genome sequencing and phylogenetic analysis of Banna virus(genus Seadornavirus,family Reoviridae)isolated from Culicoides显示文摘In an investigation of blood-sucking insects and arboviruses, a virus(YN12243) was isolated from Culicoides samples collected in the Sino-Burmese border region of Yunnan Province, China. The virus caused cytopathic effect(CPE) in C6/36 cells and passaged stably. Polyacrylamide gel analysis showed that the genome of YN12243 was composed of 12 segments of double-stranded RNA(dsRNA), with a distribution pattern of 6-6. The nucleotide and amino acid sequences of the coding region(1.12 segments) were17,803 bp and 5,925 amino acids in length, respectively. The phylogenetic analysis of VP1 protein(RdRp) revealed that YN12243 belonged to genus Seadornavirus of family Reoviridae, and further analysis indicated that YN12243 belongs to the Banna virus(BAV) genotype A2. Additionally, YN12243 was located in the same evolutionary cluster as BAV strains isolated from different mosquito species, suggesting that the BAV isolated from Culicoides does not have species barriers. These results indicate that Culicoides can also be a vector for BAV. In view of the hematophagous habits of Culicoides on cattle, horses, deer, and other large animals, as well as the possibility of spreading and causing a variety of animal arboviral diseases, it is important to improve infection detection and monitor the BAV in large livestock. | Song Song Yuanyuan Li Shihong Fu Wenwen Lei Xiaofang Guo Yun Feng Xiaoyan Gao Xiaolong Li Zhonghua Yang Ziqian Xu Ying He Huanyu Wang Hongning Zhou Bin Wang Xiaoqing Lu Guodong Liang | 2017 | Science China(Life Sciences)2017，60，12： | 8 |
| 9 | Investigation and Analysis of Rabies Viral Infection and Distribution in China in 2005-2012显示文摘Dear Editor, We report the results of a preliminary investigation of data collected between 2005 and 2012. A National Human Rabies Surveillance program was initiated in high rabies incidence regions in 2005, and subsequently carried out nationally to monitor rabies situation. Our work presents a summary of epidemiological and etiologic rabies surveillance data collected since the implementation of the program. | Wentao Jiao Hao Li Xiaoyan Tao Miao Song Xinxin Shen Zhenyang Guo Yunjiao Zhao Qing Tang Guodong Liang | 2013 | Virologica Sinica2013，28，3： | 6 |
| 10 | Antagonism of Protease-Activated Receptor 4 Protects Against Traumatic Brain Injury by Suppressing Neuroinflammation via Inhibition of Tab2/NF-κB Signaling显示文摘Traumatic brain injury(TBI)triggers the activation of the endogenous coagulation mechanism,and a large amount of thrombin is released to curb uncontrollable bleeding through thrombin receptors,also known as protease-activated receptors(PARs).However,thrombin is one of the most critical factors in secondary brain injury.Thus,the PARs may be effective targets against hemorrhagic brain injury.Since the PAR1 antagonist has an increased bleeding risk in clinical practice,PAR4 blockade has been suggested as a more promising treatment.Here,we explored the expression pattern of PAR4 in the brain of mice after TBI,and explored the effect and possible mechanism of BMS-986120(BMS),a novel selective and reversible PAR4 antagonist on secondary brain injury.Treatment with BMS protected against TBI in mice.mRNA-seq analysis,Western blot,and qRT-PCR verification in vitro showed that BMS significantly inhibited thrombin-induced inflammation in astrocytes,and suggested that the Tab2/ERK/NF-κB signaling pathway plays a key role in this process.Our findings provide reliable evidence that blocking PAR4 is a safe and effective intervention for TBI,and suggest that BMS has a potential clinical application in the management of TBI. | Jianing Luo Xun Wu Haixiao Liu Wenxing Cui Wei Guo Kang Guo Hao Guo Kai Tao Fei Li Yingwu Shi Dayun Feng Hao Yan Guodong Gao Yan Qu | 2021 | Neuroscience Bulletin2021，37，2： | 5 |
| 11 | Investigation of radionuclide~(63)Ni(Ⅱ) sequestration mechanisms on mordenite by batch and EXAFS spectroscopy study显示文摘The sorption behavior and microscopic sequestration mechanisms of radionuclide 63Ni(II) on mordenite as a function of aging time,ionic strength,initial 63Ni(Ⅱ) concentrations,solid content and coexistent electrolyte ions were investigated by the com-bination of batch and EXAFS techniques.Macroscopic experiment results show that the sorption of 63Ni(Ⅱ) is dependent on ionic strength at pH<7,and independent of ionic strength at pH>7.The sorption percentage of 63Ni(Ⅱ) on mordenite increases with increasing solid content,while the sorption capacity decreases as solid content increases.The presence of different elec-trolyte ions can enhance or inhibit the sorption of Ni(II) on mordenite in various degrees.EXAFS analysis results of the sam-ples under three different ionic strengths suggest that the retained 63Ni(Ⅱ) in these samples exists in an octahedral environment with six water ligands.In the initial period of rapid uptake,the sorption of 63Ni(Ⅱ) is dominated by the formation of in-ner-sphere surface complexes.As aging time increases,63Ni(Ⅱ) sequestration behavior tends to be mainly controlled by the formation of Ni phyllosilicate co-precipitates and/or Ni(OH)2(s) precipitates.Results for the second shell fit of the sample pre-pared at an initial 63Ni(Ⅱ) concentration of 100 mg/L indicate the possible formation of Ni polynuclear surface complexes.Both the macroscopic sorption data and the molecular level evidence of 63Ni(Ⅱ) surface speciation at the mordenite/water in-terfaces should be factored into better predictions of the mobility and bioavailability of 63Ni(Ⅱ) in environment mediums. | YANG ShiTong,SHENG GuoDong,GUO ZhiQiang,TAN XiaoLi,XU JinZhang & WANG XiangKe Key Laboratory of Novel Thin Film Solar Cells,Institute of Plasma Physics,Chinese Academy of Sciences,Hefei 230031,China | 2012 | Science China Chemistry2012，55，4： | 5 |
| 12 | HEIHE WATERSHED ALLIED TELEMETRY EXPERIMENTAL RESEARCH (HiWATER): Scientific Objectives and Experimental Design显示文摘 | Li Xin Cheng Guodong Liu Shaomin Xiao Qing Ma Mingguo Jin Rui Che Tao Liu Qinhuo Wang Weizhen Qi Yuan Wen Jianguang Li Hongyi Zhu Gaofeng Guo Jianwen Ran Youhua Wang Shuoguo Zhu Zhongli Zhou Jian Hu Xiaoli Xu Ziwei | 2013 | Bulletin of the American Meteorological Society2013，，8： | 3 |
| 13 | A NEWε-GENERALIZED PROJECTION METHOD OF STRONGLY SUB-FEASIBLE DIRECTIONS FOR INEQUALITY CONSTRAINED OPTIMIZATION显示文摘In this paper,the nonlinear optimization problems with inequality constraints are discussed. Combining the ideas of the strongly sub-feasible directions method and theε-generalized projection technique,a new algorithm starting with an arbitrary initial iteration point for the discussed problems is presented.At each iteration,the search direction is generated by a new ?-generalized projection explicit formula,and the step length is yielded by a new Armijo line search.Under some necessary assumptions, not only the algorithm possesses global and strong convergence,but also the iterative points always get into the feasible set after finite iterations.Finally,some preliminary numerical results are reported. | Jinbao JIAN Guodong MA Chuanhao GUO | 2011 | Journal of Systems Science & Complexity2011，24，3： | 3 |
| 14 | Dimericbiscognienynes B and C: New diisoprenyl-cyclohexene-type meroterpenoid dimers from Biscogniauxia sp.显示文摘Dimericbiscognienynes B and C(1 and 2), two new diisoprenyl-cyclohexene-type meroterpenoid dimers,were isolated from Biscogniauxia sp. 71-10-1-1. Their structures, including the absolute configurations,were determined by spectroscopic analyses and ECD experiments. Meroterpenoids are special natural products that originate from mixed terpenoid-nonterpenoid pathway. As a member of meroterpenoid family, diisoprenyl-cyclohexene/ane-type meroterpenoids composed of two isoprenyl chains(C5 unit)and a cyclohexene/ane moiety(C6 unit), featuring diverse skeleton structures with wide range of bioactivities. In these reported diisoprenyl-cyclohexene/ane-type meroterpenoids, only three dimers were identified. The discovery of the two new dimers added members of this rare class of meroterpenoids. | Huan Zhao Meizhi Wang Guodong Chen Dan Hu Enqing Li Yibo Qu Libing Zhou Liangdong Guo Xinsheng Yao Hao Gao | 2019 | Chinese Chemical Letters2019，30，1： | 3 |
| 15 | Numerical simulation of the seismic behavior of self-centering steel beam-column connections with bottom flange friction devices显示文摘A new type of steel moment resisting frame with bottom ? ange friction devices (BFFDs) has been developed to provide self-centering capacity and energy dissipation, and to reduce permanent deformations under earthquakes. This paper presents a numerical simulation of self-centering beam-column connections with BFFDs, in which the gap opening /closing at the beam-column interfaces is simulated by using pairs of zero-length elements with compression-only material properties, and the energy dissipation due to friction is simulated by using truss elements with specified hysteretic behavior. In particular, the effect of the friction bolt bearing against the slotted plate in the BFFDs was modeled, so that the increase in lateral force and the loss of friction force due to the bolt bearing could be taken into account. Parallel elastic-perfectly plastic gap (ElasticPPGap) materials in the Open System for Earthquake Engineering Simulation (OpenSees) were used with predef ined gaps to specify the sequence that each bolt went into the bearing and the corresponding increase in bending stiffness. The MinMax material in OpenSees is used to specify the minimum and maximum values of strains of the ElasticPPGap materials. To consider the loss of friction force due to bolt bearing, a number of parallel hysteretic materials were used, and the failure of these materials in sequence simulated the gradual loss of friction force. Analysis results obtained by using the proposed numerical model are discussed and compared with the test results under cyclic loadings and the seismic loading, respectively. | Guo Tong Song Lianglong Zhang Guodong | 2011 | Earthquake Engineering and Engineering Vibration2011，10，2： | 3 |
| 16 | The advanced development of molecular targeted therapy for hepatocellular carcinoma显示文摘Hepatocellular carcinoma(HCC),one of the most common malignant tumors in China,severely threatens the life and health of patients.In recent years,precision medicine,clinical diagnoses,treatments,and innovative research have led to important breakthroughs in HCC care.The discovery of new biomarkers and the promotion of liquid biopsy technologies have greatly facilitated the early diagnosis and treatment of HCC.Progress in targeted therapy and immunotherapy has provided more choices for precise HCC treatment.Multiomics technologies,such as genomics,transcriptomics,and metabolomics,have enabled deeper understanding of the occurrence and development mechanisms,heterogeneity,and genetic mutation characteristics of HCC.The continued promotion and accurate typing of HCC,accurate guidance of treatment,and accurate prognostication have provided more treatment opportunities and prolonged survival timelines for patients with HCC.Innovative HCC research providing an in-depth understanding of the biological characteristics of HCC will be translated into accurate clinical practices for the diagnosis and treatment of HCC. | Tao Yan Lingxiang Yu Ning Zhang Caiyun Peng Guodong Su Yi Jing Linzhi Zhang Tong Wu Jiamin Cheng Qian Guo Xiaoliang Shi Yinying Lu | 2022 | Cancer Biology & Medicine2022，19，6： | 2 |
| 17 | Baicalin and jasminoidin effects on gene expression and compatibility in the hippocampus following focal cerebral ischemia显示文摘The compound traditional Chinese medicine Qingkailing,which is an ingredient used to treat cerebral ischemia,has been limited to studies concerning single genes or single pathways.Interactions and pharmacological mechanisms of the compound ingredients(baicalin and jasminoidin) remain poorly understood.In the present study,baicalin and jasminoidin,as well as the combination,were used to separately treat mouse models of cerebral ischemia.cDNA microarray analyses of 374 cerebral ischemia-related genes were utilized to determine changes in gene-expression profiles.Arraytrack 3.40 and Ingenuity Pathway Analysis(IPA) databases were utilized to analyze changes in gene molecular functions and network path functions.Baicalin or jasminoidin alone effectively reduced infarct area,and the combination resulted in significantly better outcomes.IPA showed inhibited cell apoptosis in the baicalin group and Ca2+ channel regulation in the jasminoidin group.The combination of baicalin and jasminoidin activated HTR3A and F5 expression,regulated Ca2+ channels,activated kappa light polypeptide gene enhancer inhibitor IKBKG in B cells to control IkB kinase/nuclear factor-kB cascade,suppressed activation of inflammatory cytokine interleukin-6 receptors and activated transduction of guanine-nucleotide-binding protein(G protein) signal.Results suggested that the combination of baicalin and jasminoidin resulted in similar molecular mechanisms to baicalin and jasminoidin alone.However,novel pharmacological actions of compatibility were detected,demonstrating significant protection against cerebral ischemia. | Guo, Lin Meng, Fanyun Zhang, Guodong Zhao, Jing Zhang, Zhanjun Zhou, Caixiu Wang, Zhong | 2011 | Neural Regeneration Research2011，6，3： | 2 |
| 18 | Isolation of a strong matrix attachment region (MAR) and identification of its function in vitro and in vivo显示文摘Inclusion of MARs in transgene cassettes enhances their expression and reduces position-effect variations in the transgenic host. Four new MARs (TM2, TM3, AM1 and AM2) were isolated from tobacco and Arabidopsis by PCR method. The nuclei isolated from suspension-cultured cells of rice were used to prepare nuclear matrix. With a characterized MAR (TM1) as a positive control, the Matrix-MAR interactions were tested by an in vitro binding assay to identify the DNA sequences as MARs and their binding strength to nuclear matrix in vitro was compared. The results showed that TM2 and TM3 had stronger binding strength than TM1. To determine the functions of the four new MARs in vivo, binary vectors pBI121 carrying a uidA GUS reporter gene were modified with direct repeat MARs inserted on both sides of the reporter gene cassette and were transferred into tobaccos via Agrobacterium-mediated transformation procedure. Quantitative GUS assays of the transgenic tobaccos showed that when flanking a GUS reporter gene | Kewei Zhang Jianmei Wang Guodong Yang Xingqi Guo Fujiang Wen Decai Cui Chengchao Zheng | 2002 | Chinese Science Bulletin2002，47，23： | 2 |
| 19 | Ploidy level and performance in meiotic gynogenetic offsprings of grass carp using UV-irradiated blunt snout bream sperm显示文摘Diploid(2n)gynogens,triploid(3n)hybrids and 2n hybrids fish were produced in meiotic gynogenetic offsprings of grass carp(Ctenopharyngodon idella)eggs using UV-irradiated blunt snout bream(Megalobrama amblycephala)sperm.Flow cytometry revealed that a total of 306(54.2%)2n gynogens,157(27.7%)2n hybrids and 102(18.1%)3n hybrids were identified at one year old stage examined.Chromosome analysis showed that 2n gynogens and 2n hybrid individuals had the same chromosome number as their parents(2n¼48),while the chromosome number of 3n hybrids was 3n¼72.The morphological characters of 2n hybrids and 3n hybrids were intermediate between their parents,and the 2n gynogens were similar to grass carp.Microsatellite genetic analysis showed that the expected heterozygosity(He)of 2n gynogens,2n hybrids,3n hybrids and grass carp control were 0.3775,0.6518,0.6601,0.6502,respectively,and the polymorphism information content(PIC)was 0.3791,0.5749,0.5907,0.5738,respectively.Compared with 2n and 3n hybrids,the He and PIC of 2n gynogens were significantly decreased,indicating that high homozygous could be obtained by the gynogenetic method.The 3n hybrids grew faster than both 2n gynogens and 2n hybrid individuals,suggesting that 3n hybrids had an obvious growth advantage. | Guodong Zheng Chenglong Wang Dandan Guo Xiayun Jiang Shuming Zou | 2017 | Aquaculture and Fisheries2017，2，5： | 2 |
| 20 | Molecular Characterization of China Human Rabies Vaccine Strains显示文摘To understand the molecular characteristics of China human rabies vaccine strains, we report the full-length genome of the aG strain and present a comprehensive analysis of this strain and almost all available lyssavirus genomes (58 strains) from GenBank (as of Jan 6, 2011). It is generally considered that the G protein plays a predominant role in determining the pathogenicity of the virus, to this end we predicted the tertiary structure of the G protein of aG strain, CTN181 strain and wild type strain HN10 based on the crystal structure of Vesicular stomatitis virus (VSV) G. The predicted RABV G structure has a similar topology to VSV G and the ectodomain can be divided into 4 distinct domains DI-DIV. By mapping the characterized mutations to this structure between China vaccine strains and their close street strains, we speculate that the G303(P-H) mutations of CTN181 and HN10 causing DⅡ 3D change may be associated with the attenuated virulence in both strains. Specifically, the two signature mutations (G165P and G231P) in the aG strain are withinβsheets, suggesting that both sites are of structural importance. | Xiaoyan Tao Na Han Zhenyang Guo Qing Tang Simon Rayner Guodong Liang | 2013 | Virologica Sinica2013，28，2： | 2 |
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