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| 1 | H. pylori Escape Host Immunoreaction Through Inhibiting ILK Expression by VacA显示文摘Helicobacter pylori (H。pylori ) 尽管有精力旺盛的有免疫力的回答,固执地开拓殖民地于胃的 mucosa。Vacuolating 细胞毒素由 H 藏匿了。pylori 结果是有势力 immunomodulatory 毒素,而是包含的信号 transduction 小径没在巨噬细胞被学习。我们在这研究观察了那 vacA 缺乏的 H。pylori 导致了连接 integrin 的 kinase (同类) 和 endothelial 的显著地更高的表示氮的氧 synthase (eNOS ) ,并且显著地在 monocyte/macrophage-like U937 房间的反应的氧种类(ROS ) 的更多的生产,同样与 isogenic vacA+H 相比。pylori。在 U937 房间 overexpressing 同类的 eNOS mRNA 的表示显著地与空向量与那些 transfected 相比被增加。因此, vacA 缺乏的 H。pylori 出现到 upregulate 同类表示,调制 eNOS 并且作为结果的表示,刺激 ROS 的生产。,帮助 H 是 VacA 由禁止同类表示阻止如此的进程。pylori 逃跑主人免疫反应。这机制解释,至少部分地, H 的坚持的感染。在胃的 pylori。 | Jianping Yuan Ping Li Jing Tao Xiaodong Shi Baoyu Hu Huabiao Chen Xiaokui Guo | 2009 | Cellular & Molecular Immunology2009，6，3： | 12 |
| 2 | Analysis of the microbiota of sputum samples from patients with lower respiratory tract infections显示文摘唾沫是从患更低的呼吸的道感染的病人收集的最普通的样品,它为这些感染的细菌的鉴定是关键的。在这研究,我们与更低的呼吸的道感染从 101 个病人注册了 101 件唾沫样品。开始,在唾沫包含的细菌的 16S rDNA V3 hypervariable 区域的 pyrosequencing 为 microbiota 分析作为一条文化无关的途径被利用。为比较,用一个文化依赖者的临床的实验室测试为样品也被做的唾沫的一样的队自动化了细菌的鉴定系统。由 pyrosequencing, > 70,000 DNA 碎片在被 Ribosomal 数据库工程(RDP ) 过程分析以后被发现并且分类进 129 个细菌的类。大多数序列属于几个占优势的类,例如链球菌和葡萄球菌,显示这些类起在更低的呼吸的道感染的一个重要作用。另外,属于潜在的原因的代理人的一些序列例如霉浆菌, Haemophilus,和 Moraxella,也被发现,但是这些序列没被临床的实验室测试发现。为两个方法检测的九个类,方法的敏感被比较,结果证明 pyrosequencing 是更敏感的,除了 Klebsiella 和 Mycobacterium。显著地,这个方法揭示了更复杂的细菌的社区,它为细菌的察觉显示出一个有希望的能力。 | Yuhua Zhou Ping Lin Qingtian Li Lizhong Han Huajun Zheng Yanxia Wei Zelin Cui Yuxing Ni Xiaokui Guo | 2010 | Acta Biochimica et Biophysica Sinica2010，42，10： | 8 |
| 3 | Cloning and characterization of three cheB genes in Leptospira interrogans显示文摘活动性和趋化性系统为 leptospires 的毒力是批评的。有在 leptospires 定位的通常认为的趋化性相当或相同的事物的多重拷贝大染色体。从螺旋体 interrogans 紧张 Lai 的 CheB1 和 CheB3 被预言有一个全球象 CheB 一样领域,但是 CheB2 被预言仅仅有一个 C 终端受动器领域。以便验证三通常认为的 cheB 基因的功能,他们被克隆进 pQE31 向量然后分别地,在野类型的 Escherichia coli 紧张 RP437 和 cheB 有缺陷者紧张 RP4972 表示了。聚集的试金的结果和 CheB1 的预言的第三的结构和 L 的 CheB3。interrogans 紧张 Lai 建议一个 N 终端规章的领域的缺席可以是原因之一让 CheB2 的失败补充 E。coli cheB 异种。而且, CheB2 在 leptospires 的相互作用网络完全连接到 CheR1 和 CheR3。一起拿,这些结果显示 CheB2 不能独自工作,并且在某些生理的条件下面,工作可以要求 CheB3 和 CheR1。趋化性基因相当或相同的事物的多重拷贝的存在建议了那 L。interrogans 紧张 Lai 可能有一条更复杂的化学感应的小径。 | Ke Dong Qingtian Li Chang Liu Yunyi Zhang Guoping Zhao Xiaokui Guo | 2010 | Acta Biochimica et Biophysica Sinica2010，42，3： | 5 |
| 4 | Prediction and systematic study of protein-protein interaction networks of Leptospira interrogans显示文摘Leptospira interrogans serovar Lai is a pathogenic bacterium that causes a spirochetal zoonosis in humans and some animals. With its complete genome sequence available, it is possible to analyze protein-protein interactions from a whole- genome standpoint. Here we combine four recently developed computational approaches (gene fusion method, gene neighbor method, phylogenetic profiles method, and operon method) to predict protein-pro- tein interaction networks of Leptospira interrogans strain Lai. Through comprehensive analysis on in- teractions among proteins of motility and chemotaxis system, signal transduction, lipopolysaccaride bio- synthesis and a series of proteins related to adhesion and invasion, we provided information for further studying on its pathogenic mechanism. In addition, we also assigned 203 previously uncharacterized proteins with possible functions based on the known functions of its interacting partners. This work is helpful for further investigating L. interrogans strain Lai. | SUN Jingchun XU Jinlin CAO Jianping LIU Qi GUO Xiaokui SHI Tieliu LI Yixue | 2006 | Chinese Science Bulletin2006，51，11： | 3 |
| 5 | New microbiota found in sputum from patients with community-acquired pneumonia显示文摘获得社区的肺病(帽子) 是医院和哪个还全身地没被讨论的细菌的社区里的一颗主要担心。从在尖锐阶段的病人的唾沫是反映它的特征的一种可存取的样品。在我们的学习,我们与帽子从 45 个病人分析了 45 件唾沫样品。从健康的人的十八件唾沫样品被选择为控制。在唾沫包含的所有细菌的 16 年代 rDNA V3 hypervariable 区域的 Pyrosequencing 被用作一个文化无关的方法揭示社区体质。另外,我们为在唾沫的获得医院的肺病(幸运) 的出版数据被用于比较。由 pyrosequencing, > 90,000 DNA 读被检测。在被工具在 Ribosomal 数据库工程分析以后, reads 被分类进五个主要的数并且 > 100 个类。在数水平,读的帽子的分发类似于健康的人并且在类水平的,每个类的出现在三个范畴拥有他们的特征。象链球菌和 Neisseria 那样的类在他们的百分比显示出稳定性,显示如此的类被外长的细菌或抗菌素很少影响。象在帽子的 Moraxella 和 Rothia 那样的另外的类的角色应该被强调。根据我们的分析,当时,帽子的细菌的社区与细微变化与那些相比健康的人,但是有在幸运之间的大差距。同时, Rothia 可能是一个重要内长的引起肺病的因素。 | Chunyan Chen Tian Shen Fei Tian Ping Lin Qingtian Li Zelin Cui Yajuan Zhang Min Xue Jing Ye Xiaokui Guo YuhuaZhou | 2013 | Acta Biochimica et Biophysica Sinica2013，45，12： | 3 |
| 6 | High prevalence of pathogenic Leptospira in wild and domesticated animals in an endemic area of China显示文摘Objective:To assess the prevalence of Leptospira detected in wildlife and domesticated animals in Jiangxi Province,China,in 2009.Methods:Urine samples from 28 buffaloes and kidney samples from 50 pigs,50 dogs and 38 rats were collected from Fuliang and Shangrao County,Jiangxi Province,China,in October 2009.Polymerase chain reaction(PCR) and culture analyses were used to detect Leptospira.The cultured isolates were typed using the microscopic agglutination lest(MAT).Results:The results showed that rats potentially serve as the main reservoir of leptospiral infection,followed by dogs.Although 16%of rats(6/38) were positive using culture analysis,PCR analysis using the diagnostic primers G1/G2 and B64Ⅰ/B64Ⅱor lipLS2 showed identification as 50%and 24%,respectively,of the rat samples as positive for the presence of leptospiral DNA.Conclusions:PCR-based detection of leptospiral DNA in infected kidney tissues of reservoirs is more efficient when using G1/G2 primers than lipL32 primers. However,the latter primers have a potential application for detection in urine samples.The alarmingly high prevalence of leptospiral DMA in the wild rat population near human habitation underscores the utility of routine Leptospira surveillance,preferably using PCR methods,which are more sensitive than traditional culture-based methods. | Wang Yalin Zeng Lingbing Yang Hongliang Xu Jianmin Zhang Xiangyan Guo Xiaokui Pal Utpal Qin Jinhong | 2011 | Asian Pacific Journal of Tropical Medicine2011，4，11： | 2 |
| 7 | Global antimicrobial resistance:a system-wide comprehensive investigation using the Global One Health Index显示文摘Background:Antimicrobial resistance(AMR)is one of the top ten global public health challenges.However,given the lack of a comprehensive assessment of worldwide AMR status,our objective is to develop a One Health-based system-wide evaluation tool on global AMR.Methods:We have further developed the three-hierarchical Global One Health Index(GOHI)-AMR indicator scheme,which consists of five key indicators,17 indicators,and 49 sub-indicators,by incorporating 146 countries'data from diverse authoritative databases,including WHO's Global Antimicrobial Resistance and Use Surveillance System(GLASS)and the European CDC.We investigated the overall-or sub-rankings of GOHI-AMR at the international/regional/national levels for data preprocessing and score calculation utilizing the existing GOHI methodology.Additionally,a correlation analysis was conducted between the GOHI-AMR and other socioeconomic factors.Results:The average GOHI-AMR score for 146 countries is 38.45.As expected,high-income countries(HICs)outperform the other three income groups on overall rankings and all five key indicators of GOHI-AMR,whereas lowincome countries unexpectedly outperform upper-middle-income countries and lower-middle-income countries on the antibiotics-resistant key indicator(ARR)and ARR-subordinate indicators,including carbapenem-,β-lactam-,and quinolone resistance,and even HICs on aminoglycoside resistance.There were no significant differences among the four groups on the environmental-monitoring indicator(P>0.05).GOHI-AMR was positively correlated with gross domestic product,life expectancy,and AMR-related publications,but negatively with natural growth rate and chronic respiratory disease.In contrast to Cyprus,the remarkably lower prevalence of'ESKAPE pathogens'in high-scoring Sweden and Denmark highlights Europe's huge gaps.China and Russia outperformed the other three BRICS countries on all key indicators,particularly India's ARR and Brazil's AMR laboratory network and coordination capacity.Furthermore,significant internal disparities in carbapenem-resistant Klebsiella pneumoniae(CRKP)and methicillin-resistant Staphylococcus aureus(MRSA)prevalence were observed between China and the USA,with MRSA prevalence both gradually declining,whereas CRKP prevalence has been declining in the USA but increasing in China,consistent with higher carbapenems-related indicator'performance in USA.Conclusions:GOHI-AMR is the most comprehensive tool currently available for the assessment of AMR status worldwide.We discovered unique features impacting AMR in each country and offered precise recommendations to improve the capacity to tackle AMR in low-ranking countries. | Nan Zhou Zile Cheng Xiaoxi Zhang Chao Lv Chaoyi Guo Haodong Liu Ke Dong Yan Zhang Chang Liu Yunfu Chang Sheng Chen Xiaokui Guo Xiao-Nong Zhou Min Li Yongzhang Zhu | 2022 | Infectious Diseases of Poverty2022，11，4： | 2 |
| 8 | Re-characterization of an extrachromosomal circular plasmid in the pathogenic Leptospira interrogans serovar Lai strain 56601显示文摘在中国,(str.56601 ) 螺旋体 interrogans serovar Lai 紧张 56601 是在人和动物引起严重细螺旋体病的主要病原的紧张之一。完全在 2003 定序的这 organismwas 的染色体。在 2011,然而,我们由于广泛地在螺旋体染色体散布的重复序列在 str.56601 染色体识别了并且改正一些集会错误。在这研究,我们重新分析以前报导在染色体 andrectified 的活动、噬菌体相关的 genomic 岛用各种各样的试验性的方法在 plasmid 和染色体详细说明了顺序信息。分开的圆形的 extrachromosomal plasmid 也是的 presenceof 证实岛在 L 报导了,并且它在 genomic 区域的地点是到 genomic 的 determinedrelative。由基于的脉搏地胶化电气泳动和 plasmid 的联合的 interrogans serovar Lai 基于抽取的南部的污点 analysis.This 报告证实分开的 extrachromosomal 通报 plasmid 不集成于 L 的染色体。interrogans str.56601 并且显著地改进了我们 L 的 genomic 组织,进化,和致病的理解。interrogans。特别地,这 extrachromosomal 通报 plasmid 的描述将在病原的螺旋体种类贡献 geneticmanipulation 系统的开发。 | Lili Huang Weinan Zhui Ping He Yan Zhang Xuran Zhuang Guoping Zhao Xiaokui Guo Jinhong Qin Yongzhang Zhu | 2014 | Acta Biochimica et Biophysica Sinica2014，46，7： | 1 |
| 9 | Attachment of Staphylococcus aureus is required for activation of nuclear factor kappa B in human osteoblasts显示文摘原子因素 kappa B (NF-B ) 在传染疾病的致病起一个突出的作用。葡萄球菌 aureus (S。aureus ) ,它能属于并且入侵人的造骨细胞,是骨髓炎的最普通的原因的代理人。决定是否 S。 aureus 能在人的造骨细胞激活 NF-B 并且响应感染探索激活的可能的因素,我们使用了流动 cytometry ,连接酶的 immunosorbent 试金, immunoblots ,和 electrophoretic 活动性移动确定的试金细菌的侵略,测量文化上层清液的 interleukin-6 ( IL-6 ),并且在人的造骨细胞调查 IB 降级和 NF-B 激活。而且,我们由阻止 S 探索了为 NF-B 的激活负责的可能的因素。从身体上摸人的造骨细胞或禁止 S 的侵略的 aureus。进在合作文化下面的人的造骨细胞的 aureus 调节,由孵化对待 K 的朊酶或紫外杀死的 S。有人的造骨细胞并且由与 peptidoglycan (PGN ) 或 lipoteichoic 酸(LTA ) 对待人的造骨细胞的 aureus。我们发现了那 S。aureus 导致了 IB 降级和 NF-B 激活,它能响应感染在人的造骨细胞的文化上层清液调整 IL-6 分泌物。另外,在人的造骨细胞的最大的 IB 降级和 NF-B 激活发生在 S 的最大的侵略以前。aureus。它不是附件侵略或分泌可溶的因素, PGN, S 的 LTA。aureus,那能在人的造骨细胞导致 IB 降级和 NF-B 激活。这些结果显示了那 S。aureus 能在人的造骨细胞激活 NF-B 并且 S 的附件。aureus 响应感染为这激活被要求。 | Rende Ning Xianlong Zhang Xiaokui Guo Qingtian Li | 2010 | Acta Biochimica et Biophysica Sinica2010，42，12： | 1 |
| 10 | Leptospira interrogans encodes an ROK family glucokinase involved in a cryptic glucose utilization pathway显示文摘尽管螺旋体 interrogans 是不能的作为它的碳 / 精力利用葡萄糖,采购原料, L 的 LA_1437 基因。interrogans serovar Lai 潜在地编码组 III glucokinase (GLK ) 。L。interrogans GLK (LiGLK ) 不同类地在 Escherichia 表示了 coli 被净化并且证明了是有它 12.3 的特定的活动的 homodimeric 酶 ? 桴 ? 慰瑲慩?? 潤慭湩愠摮渠牯慭?? 整浲湩獵? 獩猠潨瑲牥琠慨 ? 偁 ? 湡 ??? 戠 ? 桴 ? 敲獡湯漠 ? 敧敮映慲敭桳晩 ? 畭慴楴湯搠牵湩? | Qing Zhang Yunyi Zhang Yi Zhong Jun Ma Nanqiu Peng Xingjun Cao Chen Yang RongZeng Xiaokui Guo Guoping Zhao | 2011 | Acta Biochimica et Biophysica Sinica2011，43，8： | 1 |
| 11 | Key immunity characteristics of diverse stages of brucellosis in rural population from Inner Mongolia, China显示文摘Background: Brucellosis poses a serious threat to human and animal health,particularly in developing countries such as China.The Inner Mongolia Autonomous Region is one of the most severely brucellosis-endemic provinces in China.Currently,the host immune responses functioning to control Brucella infection and development remain poorly understood.The aim of this study is to further clarify the key immunity characteristics of diverse stages of brucellosis in Inner Mongolia.Methods: We collected a total of 733 blood samples from acute(n=137),chronic(n=316),inapparent(n=35),recovery(n=99),and healthy(n=146)groups from the rural community of Inner Mongolia between 2014 and 2015.The proportions of CD4^(+),CD8^(+),Th1,Th2,and Th17 T cells in peripheral blood and the expression of TLR2 and TLR4 in lymphocytes,monocytes and granulocytes were examined using flow cytometry analysis.The differences among the five groups were compared using one-way ANOVA and the Kruskal–Wallis method,respectively.Results: Our results revealed that the proportions of CD4^(+) and CD8^(+) T cells were significantly different among the acute,chronic,recovery,and healthy control groups(P<0.05),with lower proportions of CD4^(+) T cells and a higher proportion of CD8^(+) T cells in the acute,chronic,and recovery groups.The proportion of Th1 cells in the acute,chronic,and inapparent groups was higher than that in the healthy and recovery groups;however,there was no significant difference between patients and healthy individuals(P>0.05).The proportion of Th2 lymphocytes was significantly higher in the acute and healthy groups than in the inapparent group(P<0.05).The proportion of Th17 cells in the acute group was significantly higher than that in the healthy control,chronic,and inapparent groups(P<0.05).Finally,the highest expression of TLR4 in lymphocytes,monocytes and granulocytes was observed in the recovery group,and this was followed by the acute,chronic,healthy control,and inapparent groups.There was a significant difference between the recovery group and the other groups,except for the acute group(P<0.05).Moreover,a correlation in TLR4 expression was observed in lymphocytes,monocytes and granulocytes among the five groups(r>0.5),except for the inapparent group between lymphocytes and granulocytes(r=0.34).Conclusions: Two key factors(CD8^(+)T cells and TLR4)in human immune profiles may closely correlate with the progression of brucellosis.The detailed function of TLR4 in the context of a greater number of cell types or tissues in human or animal brucellosis and in larger samples should be further explored in the future. | Yongzhang Zhu Li Shi Yige Zeng Dongri Piao Yingbo Xie Juan Du Meng Gao Wei Gao Junli Tian Jun Yue Min Li XiaoKui Guo Yufeng Yao YaoXia Kang | 2022 | Infectious Diseases of Poverty2022，11，3： | 1 |
| 12 | Genetic and molecular biological characterization of two homologous cheR genes from Leptospira interrogans显示文摘螺旋体 interrogans 染色体编码 cheR 基因的二个拷贝,他们中的每能为 Escherichia coli cheR 0 异种 RP1254 的聚集的有缺点的显型补充到某些程度,当他们的也的在表示上禁止趋化性的野类型的 E 聚集时。coli 紧张, RP437。因此, CheR1 和 CheR2 应该忍受 methyltransferase 活动,尽管 CheR1 有仅仅而不是位于 2 螺旋的断然控告的脸的二保存基本氨基酸残余。当象 CheR2 的 Lys48 和 Arg55 一样的这残余被变异时,任何一个 CheRs 都不能维持上述的互补功能,建议他们考虑到类似于 E 的接受甲基的趋化性蛋白质的关键角色。coli。由 microarray 试金示威了,在 L 的 cheR1 的表情。在 28 点有教养的 | Yunyi Zhang Ke Dong Lingbing Zeng Qingtian Li Chang Liu Jin Wang Xiaokui Guo Guo-Ping Zhao | 2013 | Acta Biochimica et Biophysica Sinica2013，45，10： | 1 |
| 13 | Functional characterization of RelBE toxin-antitoxin system in probiotic Bifidobacterium Iongum JDM301显示文摘毒素抗毒素(TA ) 系统在细菌和 archaea 是普遍的。然而,在细菌的生理学的 chromosomally 编码的 TA 系统的角色仍然是开的辩论。在这研究, ? TA ? 在 Bifidobacterium longum JDM301 的 module-relBE (relBE Bif ) 被识别,它在压力反应的功能被评估。JDM301 的整个染色体序列的生物信息学分析揭示了编码一个象 RelBE 一样 TA 系统的一双连接基因(RelBE Bif ) 。我们的结果揭示了 relBE 在 JDM301 的 Bif 。RelE Bif 在 Escherichia coli 上有有毒的效果,它能被它的血缘的抗毒素的合作表示抵销, RelB Bif 。我们的数据也表明了那 RelE Bif 是 mRNA interferase 并且 RelE Bif 能被 RelB Bif 。这些结果建议那 RelE Bif 是通过 mRNA 降级逮捕细胞生长的有毒的核酸酶,并且 RelE Bif 能被 RelB Bif 。另外,我们也发现 RelBE Bif 在渗透的应力期间被增加,建议那 RelBE Bif 在这个不利条件下面被激活。我们的结果暗示 RelBE Bif TA 模块可以代表帮助 B 的一个房间生长调节的人。longum 将处理渗透的应力。 | Yanxia Wei Lu Ye Yang Li Fan Yang Dianbin Liu Xiaokui Guo Renxian Tang Chang Liu | 2016 | Acta Biochimica et Biophysica Sinica2016，48，8： | 1 |
| 14 | Consensus on potential biomarkers developed for use in clinical tests for schizophrenia显示文摘Background Schizophrenia is a serious mental illness affecting approximately 20 million individuals globally.Both genetic and environmental factors contribute to the illness.If left undiagnosed and untreated,schizophrenia results in impaired social function,repeated hospital admissions,reduced quality of life and decreased life expectancy.Clinical diagnosis largely relies on subjective evidence,including self-reported experiences,and reported behavioural abnormalities followed by psychiatric evaluation.In addition,psychoses may occur along with other conditions,and the symptoms are often episodic and transient,posing a significant challenge to the precision of diagnosis.Therefore,objective,specific tests using biomarkers are urgently needed for differential diagnosis of schizophrenia in clinical practice.Aims We aimed to provide evidence-based and consensus-based recommendations,with a summary of laboratory measurements that could potentially be used as biomarkers for schizophrenia,and to discuss directions for future research.Methods We searched publications within the last 10 years with the following keywords:‘schizophrenia’,‘gene’,‘inflammation’,‘neurotransmitter’,‘protein marker’,‘gut microbiota’,‘pharmacogenomics’and‘biomarker’.A draft of the consensus was discussed and agreed on by all authors at a round table session.Results We summarised the characteristics of candidate diagnostic markers for schizophrenia,including genetic,inflammatory,neurotransmitter,peripheral protein,pharmacogenomic and gut microbiota markers.We also proposed a novel laboratory process for diagnosing schizophrenia in clinical practice based on the evidence summarised in this paper.Conclusions Further efforts are needed to identify schizophrenia-specific genetic and epigenetic markers for precise diagnosis,differential diagnosis and ethnicity-specific markers for the Chinese population.The development of novel laboratory techniques is making it possible to use these biomarkers clinically to diagnose disease. | Ping Lin Junyu Sun Xiaoyan Lou Dan Li Yun Shi Zhenhua Li Peijun Ma Ping Li Shuzi Chen Weifeng Jin Shuai Liu Qing Chen Qiong Gao Lili Zhu Jie Xu Mengyuan Zhu Mengxia Wang Kangyi Liang Ling Zhao Huabin Xu Ke Dong Qingtian Li Xunjia Cheng Jinghong Chen Xiaokui Guo | 2022 | General Psychiatry2022，35，1： | 1 |
| 15 | Simultaneous emergence of several biotypes of Brucella among Baotou's brucellosis patients by combined diagnostic strategy显示文摘 | Yaoxia Kang Enhou Jia Liang Lin Xiangyang Duan Pei Xiao Jingxian Peng Wei Gao Chaobao Zhang Xiaokui Guo Buyun Cui Xiangqi Li | 2013 | Acta Biochimica et Biophysica Sinica2013，45，10： | 0 |
| 16 | Dynamic antimicrobial resistant patterns of Escherichia coli from healthy poultry and swine over 10 years in Chongming Island,Shanghai显示文摘Background:Antimicrobial resistance(AMR)is one of the greatest threats to animal and public health.Here,we conducted a dynamic surveillance of Escherichia coli on Chongming Island in Shanghai during 2009-2021 to identify the characteristics and trends of Chongming’s AMR pandemic.Methods:Rectal(cloaca)swabs from four poultry and nine swine farms(Chongming Island,2009-2021)were collected for E.coli strains acquisition.The micro-broth dilution method was used to test antimicrobial susceptibility of E.coli isolates against 10 antimicrobial classes including 15 antimicrobials.Utilizing generalized linear mixed models(GLMMs)and co-occurrence analyses,we further explored the multiple-drug-resistance(MDR)combinations and dynamic patterns of E.coli over 10 years in two food animals.Results:Total of 863 MDR isolates were found among 945 collected E.coli isolates,337 from poultry and 608 from swine.Both isolates exhibited high resistant rates(>70%)to tetracyclines,phenicols,sulfonamides,penicillins,and aminoglycosides(only in swine).The resistant rates of swine isolates to penicillins,aminoglycosides,tetracyclines,phenicols,and polymyxins were significantly higher than those of poultry isolates,whereas resistance to fluoroquinolones was reversed.Resistance to polymyxins decreased similarly in swine(42.4%in 2009 to 0.0%in 2021)and poultry isolates(from 16.5%to 0.0%).However,resistance to other seven antimicrobial classes(excluding carbapenems and penicillins)declined dramatically in swine isolates,particularly fluoroquinolones(from 80.5%to 14.4%),and tendencies of resistance to the seven classes showed markedly divergent patterns in poultry isolates.Using Poisson GLMMs,the AMR carriage since 2016 was significantly lower than that of 2009(odds ratio<1),indicating a decline in the risk of MDR emergence.Furthermore,despite the highly diverse MDR profiles,co-occurrence analysis identified two prominent MDR clusters of penicillins-phenicols-fluoroquinolones in poultry and aminoglycosides-tetracyclines-sulfonamides-phenicols in swine.Conclusions:Our study uncovered vastly distinct AMR patterns and dynamic tendencies of poultry and swine E.coli isolates from Chongming.Meanwhile,Chongming’s AMR status has ameliorated,as indicated by the decline in antimicrobials prevalence(particularly in swine),lower likelihood of MDR emergence and low carbapenem-,cephalosporin-,and polymyxin resistance.Importantly,this surveillance results are the vital basis for future policy development in Chongming and Shanghai. | Chao Lv Jun Shang Wengang Zhang Bingqing Sun Min Li Chaoyi Guo Nan Zhou Xiaokui Guo Shixin Huang Yongzhang Zhu | 2022 | Infectious Diseases of Poverty2022，11，5： | 0 |
| 17 | Correction: Characterization of a small-molecule inhibitor targeting NEMO/IKKβ to suppress colorectal lcancergrowth显示文摘In the process of collating the raw data,the authors noticed an inadvertent mistake occurred in Fig.3b that needs to be corrected after online publication of the article.In Fig.3b,as a result of an error in the graphics panel arrangement process,the band of NEMO was repeatedly inserted asβ-actin by mistake.The correct band is shown as below and in the updated Fig.3b.The correction did not affect any of our results or discussion as present in the original publication.We regret any inconvenience this has caused. | Zhenlong Yu Jian Gao Xiaolei Zhang Yulin Peng Wenlong Wei Jianrong Xu Zhenwei Li Chao Wang Meirong Zhou Xiangge Tian Lei Feng Xiaokui Huo Min Liu Mingliang Ye De-an Guo Xiaochi Ma | 2023 | Signal Transduction and Targeted Therapy2023，8，10： | 0 |
| 18 | Characterization of a small-molecule inhibitor targeting NEMO/IKKβto suppress colorectal cancer growth显示文摘NEMO/IKKβcomplex is a central regulator of NF-κB signaling pathway,its dissociation has been considered to be an attractive therapeutic target.Herein,using a combined strategy of molecular pharmacological phenotyping,proteomics and bioinformatics analysis,Shikonin(SHK)is identified as a potential inhibitor of the IKKβ/NEMO complex.It destabilizes IKKβ/NEMO complex with IC_(50) of 174 nM,thereby significantly impairing the proliferation of colorectal cancer cells by suppressing the NF-κB pathway in vitro and in vivo.In addition,we also elucidated the potential target sites of SHK in the NEMO/IKKβcomplex.Our study provides some new insights for the development of potent small-molecule PPI inhibitors. | Zhenlong Yu Jian Gao Xiaolei Zhang Yulin Peng Wenlong Wei Jianrong Xu Zhenwei Li Chao Wang Meirong Zhou Xiangge Tian Lei Feng Xiaokui Huo Min Liu Mingliang Ye De-an Guo Xiaochi Ma | 2022 | Signal Transduction and Targeted Therapy2022，7，4： | 0 |
| 19 | Characterization of acetyl-CoA and propionyl-CoA carboxylases encoded by Leptospira interrogans serovar Lai: an initial biochemical study for leptospiral gluconeogenesis via anaplerotic CO2 assimilation显示文摘螺旋体 interrogans 是细螺旋体病的原因的代理人。在里面 L 的 vitro 生长。interrogans 要求 CO2 和包含二 acyl-CoA carboxylases 的一条部分 3-hydroxypropionate 小径被 genomic 分析建议吸收 CO2。候选人基因的任何一个集合 heterologously 在 Escherichia coli 共同表示能表明两 acetyl-CoA carboxylase (ACC ) 和 propionyl-CoA carboxylase (PCC ) 活动。尽管没能被净化, tri 子单元 holoenzyme (LA_2736-LA_2735 和 LA_3803 ) 基于它向 acetyl-CoA 的底层偏爱是指明的 ACC。部分净化的双性人子单元 holoenzyme (LA_2432-LA_2433 ) 比 acetyl-CoA,并且这样,指明了 PCC 的作为底层对 propionyl-CoA 举办一项更加更高的活动。本国的 polyacrylamide 胶化电气泳动显示这 PCC 有约 669 kDa 的一个分子的团,建议 44 第四级的结构和当模特儿的结构的相同和它的 carboxyltransferase 子单元( LA_2433 )的指导地点的 mutagenesis 分析显示在通常认为的底层绑定衣袋的底部定位的 A431 残余可以在底层特性决心起一个重要作用。transcriptomic 和 proteomic 数据显示涉及建议部分 3-hydroxypropionate 小径的酶除了 ACC/PCC 在 vivo 被表示,在另外的螺旋体种类的染色体的相应基因因此被重新注解。然而,在里面 vitro 在粗略的细胞摘录检测了 ACC 的特定的活动太低,不能在 EllinghausenMcCulloughJohnsonHarris 最小的媒介说明细菌的生长,进一步系统的分析被要求经由 anaplerotic CO2 揭开 gluconeogenesis 的机制在螺旋体的吸收种。 | Nanqiu Peng Yi Zhong Qing Zhang Mingyue Zheng Wei Zhao Hualiang Jiang Chen Yang Xiaokui Guo Guoping Zhao | 2012 | Acta Biochimica et Biophysica Sinica2012，44，8： | 0 |
| 20 | Molecular Identification and Genetic Characterization of Public Health Threatening Ticks — Chongming Island, China, 2021–2022显示文摘Summary What is already known about this topic?Although ticks and tick-borne diseases are prevalent throughout China,there remains a knowledge gap regarding their biology and potential risk of distribution to human and animal populations on Chongming Island.The island,being China’s third largest and a crucial component in the ecological preservation of the Yangtze Delta region,has yet to be comprehensively studied in this context. | Siwei Fei Hanqing Zhao Jingxian Yin Li Wang Zhishan Sun Wenge Zhang Yan Zhang Ke Dong Shan Lyu Xiaokui Guo Xiao-nong Zhou Kokouvi Kassegne | 2023 | China CDC weekly2023，5，37： | 0 |
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