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| 1 | One-step generation of p53 gene biallelic mutant Cynomolgus monkey via the CRISPR/Cas system显示文摘 | Haifeng Wan Chunjing Feng Fei Teng Shihua Yang Baoyang Hu Yuyu Niu Andy Peng Xiang Weizhen Fang Weizhi Ji Wei Li Xiaoyang Zhao Qi Zhou | 2015 | Cell Research2015，25，2： | 39 |
| 2 | Widespread Whole Genome Duplications Contribute to Genome Complexity and Species Diversity in Angiosperms显示文摘基因复制为产生新奇功能提供进化潜力,当 polyploidization 或整个染色体复制(WGD ) 开始加倍染色体并且导致百~千个保留的副本时。WGD 被通常在优核质的许多充满种类的系发现的证据强烈支持,并且因此在种类多样化被看作一个主要驱动力。我们执行了 59 公共 genomes/transcriptomes 和盖住被子植物的主要的系的 46 最新定序的 transcriptomes 的比较 genomic 和 phylogenomic 分析 ? 由调查几万个基因家谱检测大规模基因复制事件。这些分析证实了大多数以前报导的 WGD 并且在许多系为新奇的提供了充分证据。检测 WGD 与约 2160 万年的估计的半衰期在进化期间支持了指数的基因损失的一个模型,并且与两个被相关有多样化的高度和全球气候的时期的系的出现变化。新数据集和分析 ? 检测了广泛地在支持被子植物放射并且提高他们的改编到环境变化为 WGD 的角色在被子植物进化,在必要细胞的新陈代谢的基因功能的揭开的优先的保留,和提供的线索期间散布的许多新奇 WGD。 | Ren Ren Haifeng Wang Chunce Guo Ning Zhang Liping Zeng Yamao Chen Hong Ma Ji Qi | 2018 | Molecular Plant2018，11，3： | 35 |
| 3 | Atg7 is required for acrosome biogenesis during spermatogenesis in mice显示文摘acrosome 是盖住精子原子核的前面的部分并且在授精的过程起一个必要作用的一个专业化细胞器。位于这个 lysosome 相关的细胞器(LRO ) 的生物的续生说下面的分子的机制大部分仍然是未知的。这里,我们显示出房间特定的 Atg7 大美人老鼠是的那个细菌由于在 acrosome 生物的续生说的一个缺点不肥沃并且显示了类似于人的 globozoospermia 的显型;这个繁殖缺点被 intracytoplasmic 精子注射成功地救。而且,在细菌房间的 Atg7 的弄空没影响细菌房间的开发的早阶段,但是精子发生在以后上演,在 Golgi 期间没有通过到保险丝进一个单个 acrosomal 泡的 proacrosomal 泡分阶段执行,它最后导致了不规则或将近 round-headed 精子。Autophagic 流动在弄空 Atg7 的细菌房间被破坏,最后导致 LC3 变化形式的失败到 Golgi 导出仪器的泡。另外, Atg7 部分调整了另一 globozoospermia 相关的蛋白质,联系 Golgi 的 PDZ 卷和卷卷包含主题的蛋白质(GOPC ) ,在 acrosome 生物的续生说期间。最后,任何一个 autophagy 的注射或进睾丸的 lysosome 禁止者导致了类似的显型到细菌的房间特定的 Atg7 大美人老鼠。总的来说,我们的结果揭开为在 acrosome 的生物的续生说的 Atg7 的一个新角色,并且我们提供证据为 acrosome 支持 autolysosome 起源假设。 | Hongna Wang Haifeng Wan Xixia Li Weixiao Liu Qi Chen Yaqing Wang Lin Yang Hongmei Tang Xiujun Zhang Enkui Duan Xiaoyang Zhao Fei Gao Wei Li | 2014 | Cell Research2014，24，7： | 25 |
| 4 | Birth of fertile bimaternal offspring following intracytoplasmic injection of parthenogenetic haploid embryonic stem cells显示文摘 | Zhikun Li Haifeng Wan Guihai Feng Leyun Wang Zhengquan He YukaiWang Xiu-Jie Wang Wei Li Qi Zhou Baoyang Hu | 2016 | Cell Research2016，26，1： | 15 |
| 5 | Ablation and radar-wave transmission performances of the nitride ceramic matrix composites显示文摘The 2.5 dimensional silica fiber reinforced nitride matrix composites (2.5D SiO2f/Si3N4-BN) were prepared through the preceramic polymer impregnation pyro- lysis (PIP) method. The ablation and radar-wave transparent performances of the composite at high temperature were evaluated under arc jet. The composition and ablation surface microstructures were studied by X-ray diffraction (XRD) and scanning electron microscope (SEM). The results show that the 2.5D SiO2f/Si3N4-BN composites have a linear ablation rate of 0.33 mm/s and high radar-wave trans- parent ratio of 98.6%. The fused layer and the matrix are protected by each other, and no fused layer accumulates on the ablation surface. The nitride composite is a high-temperature ablation resistivity and microwave transparent material. | JIANG YongGang ZHANG ChangRui CAO Feng WANG SiQing HU HaiFeng QI GongJin | 2008 | Science China(Technological Sciences)2008，51，1： | 14 |
| 6 | Analysis on the hourly spatiotemporal complementarities between China's solar and wind energy resources spreading in a wide area显示文摘China is rich in solar and wind energy resources,of which the proportion of China's power sources has been rapidly increasing.Such fluctuating and intermittent energy sources will bring significant challenges to the safe and stable operation power system.However,making use of the spatiotemporal complementarities between different renewable energy resources is a feasible way to level fluctuating power especially when they have a widely geographical dispersion.Based on the data provided by China Meteorological Administration(CMA),this research explores the spatiotemporal complementarities between wind and solar energy resources.This paper nondimensionalizes hourly wind speed and global solar radiation data and employs several indexes to compare the smoothing effect with various combining scenarios.The results show that combining wind and solar powers within a certain area can cause a fall of zero-power or very-full power hours.Besides,combining different resources improves 'smoothness' in power output when compared with that from each individual resource.However,under hourly time scale,when the dispersion of sites is large enough,the smoothing effect of combining the dispersed wind power is very close to the combination of those two different resources.Nevertheless,this complementary effect is much better than that of just combining solar energy resources. | LIU Yi XIAO LiYe WANG HaiFeng DAI ShaoTao QI ZhiPing | 2013 | Chinese Science Bulletin2013，58，7： | 14 |
| 7 | Parthenogenetic haploid embryonic stem cells produce fertile mice显示文摘 | Haifeng Wan Zhengquan He Mingzhu Dong Tiantian Gu Guan-Zheng Luo Fei Teng Baolong Xia Wei Li Chunjing Feng Xin Li Tianda Li Ling Shua Rui Fu Liu Wang Xiu-Jie Wang Xiao-Yang Zhao Qi Zhou | 2013 | Cell Research2013，23，11： | 13 |
| 8 | Successful generation of cloned mice using nuclear transfer from induced pluripotent stem cells显示文摘 | Shuya Zhou Chenhui Ding Xiaoyang Zhao Eryao Wang Xiangpeng Dai Lei Liu Wei Li Zichuan Liu Haifeng Wan Chunjing Feng Tang Hai Liu Wang Qi Zhou | 2010 | Cell Research2010，20，7： | 10 |
| 9 | Effectiveness of Inactivated COVID-19 Vaccines Against Symptomatic,Pneumonia,and Severe Disease Caused by the Delta Variant:Real World Study and Evidence—China,2021显示文摘Summary What is already known about this topic?Effectiveness of China’s 2 inactivated vaccines(BBIBPCorV and CoronaVac)against pre-Delta severe acute respiratory syndrome coronavirus-2(SARS-CoV-2)variants ranged from 47%to over 90%,depending on the clinical endpoint,and with greater effectiveness against more severe coronavirus disease 2019(COVID-19).During an outbreak in Guangdong,inactivated vaccine effectiveness(VE)against the Delta variant was 70%for symptomatic infection and 100%for severe COVID-19.However,separate or combined VE estimates for the two inactivated vaccines against Delta are not available.What is added by this report?In an outbreak that started in a hospital,VEs of completed primary vaccination with inactivated COVID-19 vaccines against symptomatic COVID-19,COVID-19 pneumonia,and severe COVID-19 caused by the Delta variant were 51%,61%,and 82%.Completed primary vaccination reduced the risk of progressing from mild to moderate or severe COVID-19 by 74%.VE estimates for BBIBP-CorV and CoronaVac or combined vaccination were similar,and partial vaccination was ineffective.What are the implications for public health practice?Completed primary vaccination with either of the 2 inactivated COVID-19 vaccines reduces risk of symptomatic COVID-19,COVID-19 pneumonia,and severe COVID-19 caused by the Delta variant.Completion of the completed primary vaccination with two doses is necessary for protection from Delta. | Dan Wu Yanyang Zhang Lin Tang Fuzhen Wang Ying Ye Chao Ma Hui Zheng Wenzhou Yu Lei Cao Yifan Song Abuduwaili Reyimu Xiaoxiao Zhang Haifeng Wang Yifei Nie Mingxia Lu Muge Qi Jun Li Ruolin Wang Kaichao Yang Changshuang Wang Lawrence Everett Rodewald Geroge Fu Gao Zhijie An Zundong Yin | 2022 | China CDC weekly2022，4，4： | 7 |
| 10 | Generation of fertile offspring from Kitw/Kitwv mice through differentiation of gene corrected nuclear transfer embryonic stem cells显示文摘 | Yan Yuan Quan Zhou Haifeng Wan Bin Shen Xuepeng Wang Mei Wang Chunjing Feng Mingming Xie Tiantian Gu Tao Zhou Rui Fu Xingxu Huang Qi Zhou Jiahao Sha Xiao-Yang Zhao | 2015 | Cell Research2015，25，7： | 7 |
| 11 | MdMYB6 regulates anthocyanin formation in apple both through direct inhibition of the biosynthesis pathway and through substrate removal显示文摘Anthocyanin biosynthesis and sugar metabolism are important processes during plant growth,but the molecular interactions underlying these pathways are still unclear.In this work,we analyzed the anthocyanin and soluble sugar contents,as well as the transcript levels of transcription factors that are known to be related to the biosynthesis of anthocyanin in‘Hongcui 1’apple flesh during fruit development.Overexpression of MdMYB6 in red-fleshed calli was found to reduce anthocyanin content and result in downregulated expression of the MdANS and MdGSTF12 proteins.Yeast one-hybrid and electrophoretic mobility shift analyses showed that MdMYB6 could directly bind to the promoters of MdANS and MdGSTF12,indicating that MdMYB6 could inhibit anthocyanin biosynthesis by regulating MdANS and MdGSTF12.Overexpression of MdTMT1 in the Arabidopsis tmt1 mutant restored the glucose and fructose contents to the wild-type levels,while overexpression of MdTMT1 in red-fleshed calli increased the contents of glucose and fructose but reduced the contents of UDP-glucose,UDP-galactose,and anthocyanin.Using a GUS reporter system,yeast one-hybrid,chromatin immunoprecipitation-PCR and electrophoretic mobility shift analyses,we found that MdMYB6 could bind to the promoter of MdTMT1,resulting in increased promoter activity.Overexpression of MdMYB6 in calli overexpressing MdTMT1 increased the expression of MdTMT1,which led to reduced contents of UDP-glucose and UDP-galactose and decreased anthocyanin content compared to those of the calli that overexpressed MdTMT1.This finding suggested that MdMYB6 could also inhibit anthocyanin biosynthesis by regulating MdTMT1 to decrease the contents of UDP-glucose and UDP-galactose.Taken together,these results showed that MdMYB6 and MdTMT1 play key roles in both anthocyanin biosynthesis and sugar transport. | Haifeng Xu Qi Zou Guanxian Yang Shenghui Jiang Hongcheng Fang Yicheng Wang Jing Zhang Zongying Zhang Nan Wang Xuesen Chen | 2020 | Horticulture Research2020，7，1： | 6 |
| 12 | Cryo-self-assembled silk fibroin sponge as a biodegradable platform for enzyme-responsive delivery of exosomes显示文摘Although advances in protein assembly preparation have provided a new platform for drug delivery during tissue engineering,achieving long-term controlled exosome delivery remains a significant challenge.Diffusion-dominated exosome release using protein hydrogels results in burst release of exosomes.Here,a fibroin-based cryo-sponge was developed to provide controlled exosome release.Fibroin chains can self-assemble into silk I structures under ice-cold conditions when annealed above the glass transition temperature.Exosome release is enzyme-responsive,with rates primarily determined by enzymatic degradation of the scaffolds.In vivo experiments have demonstrated that exosomes remain in undigested sponge material for two months,superior to their retention in fibrin glue,a commonly used biomaterial in clinical practice.Fibroin cryo-sponges were implanted subcutaneously in nude mice.The exosome-containing sponge group exhibited better neovascularization and tissue ingrowth effects,demonstrating the efficacy of this exosome-encapsulating strategy by realizing sustained release and maintaining exosome bioactivity.These silk fibroin cryo-sponges containing exosomes provide a new platform for future studies of exosome therapy. | Muyang Sun Qi Li Huilei Yu Jin Cheng Nier Wu Weili Shi Fengyuan Zhao Zhenxing Shao Qingyang Meng Haifeng Chen Xiaoqing Hu Yingfang Ao | 2022 | Bioactive Materials2022，7，2： | 4 |
| 13 | POWER CONTROL BASED COOPERATIVE OPPORTUNISTIC ROUTING IN WIRELESS SENSOR NETWORKS显示文摘This paper proposes an approach called PC-CORP (Power Control based Cooperative Opportunistic Routing Protocol) for WSN (Wireless Sensor Networks), providing robustness to the random variations in network connectivity while ensuring better data forwarding efficiency in an energy efficient manner. Based on the realistic radio model, we combine the region-based routing, rendezvous scheme, sleep discipline and cooperative communication together to model data forwarding by cross layer design in WSN. At the same time, a lightweight transmission power control algorithm called PC-AIMD (Power Control Additive Increase Multiplicative Decrease) is introduced to utilize the co- operation of relay nodes to improve the forwarding efficiency performance and increase the robustness of the routing protocol. In the simulation, the performance of PC-COPR is investigated in terms of the adaptation of variations in network connectivity and satisfying the QoS requirements of application. | Hu Haifeng Zhu Qi | 2009 | Journal of Electronics(China)2009，26，1： | 4 |
| 14 | Domesticated cynomolgus monkey embryonic stem cells allow the generation of neonatal interspecies chimeric pigs显示文摘Blastocyst complementation by pluripotent stem cell(PSC)injection is believed to be the most promising method to generate xenogeneic organs.However,ethical issues prevent the study of human chimeras in the late embryonic stage of development.Primate embryonic stem cells(ESCs),which have similar pluripotency to human ESCs,are a good model for studying interspecies chimerism and organ generation.However,whether primate ESCs can be used in xenogenous grafts remains unclear.In this study,we evaluated the chimeric ability of cynomolgus monkey(Macaca fascicularis)ESCs(cmESCs)in pigs,which are excellent hosts because of their many similarities to humans.We report an optimized culture medium that enhanced the anti-apoptotic ability of cmESCs and improved the development of chimeric embryos,in which domesticated cmESCs(D-ESCs)injected into pig blastocysts differentiated into cells of all three germ layers.In addition,we obtained two neonatal interspecies chimeras,in which we observed tissue-specific D-ESC differentiation.Taken together,the results demonstrate the capability of D-ESCs to integrate and differentiate into functional cells in a porcine model,with a chimeric ratio of 0.001-0.0001 in different neonate tissues.We believe this work will facilitate future developments in xenogeneic organogenesis,bringing us one step closer to producing tissue-specific functional cells and organs in a large animal model through interspecies blastocyst complementation. | Rui Fu Dawei Yu Jilong Ren Chongyang Li Jing Wang Guihai Feng Xuepeng Wang Haifeng Wan Tianda Li Libin Wang Ying Zhang Tang Hai Wei Li Qi Zhou | 2020 | Protein & Cell2020，11，2： | 4 |
| 15 | Pollutant toxicology with respect to microalgae and cyanobacteria显示文摘Microalgae and cyanobacteria are fundamental components of aquatic ecosystems.Pollution in aquatic environment is a worldwide problem.Toxicological research on microalgae and cyanobacteria can help to establish a solid foundation for aquatic ecotoxicological assessments.Algae and cyanobacteria occupy a large proportion of the biomass in aquatic environments;thus,their toxicological responses have been investigated extensively.However,the depth of toxic mechanisms and breadth of toxicological investigations need to be improved.While existing pollutants are being discharged into the environment daily,new ones are also being produced continuously.As a result,the phenomenon of water pollution has become unprecedentedly complex.In this review,we summarize the latest findings on five kinds of aquatic pollutants,namely,metals,nanomaterials,pesticides,pharmaceutical and personal care products(PPCPs),and persistent organic pollutants(POPs).Further,we present information on emerging pollutants such as graphene,microplastics,and ionic liquids.Efforts in studying the toxicological effects of pollutants on microalgae and cyanobacteria must be increased in order to better predict the potential risks posed by these materials to aquatic ecosystems as well as human health. | Tao Lu Qi Zhang Zhenyan Zhang Baolan Hu Jianmeng Chen Jun Chen Haifeng Qian | 2021 | Journal of Environmental Sciences2021，33，1： | 4 |
| 16 | Dinoflagellate cysts in recent marine sediment from Guangxi, China显示文摘Total of 33 species of dinoflagellate cysts were discovered from surface sediment in the searegion of Guangxi, among them 12 cyst types (Diplopsalopsis sp. 1, D. sp.2, D. sp.3, Cochlodiniumsp., Protoperidinium sp. 1, P. sp. 2, P.compressum, Scrippsiella sp. 1, S. sp. 2, Alexandriumsp. 1, A. sp. 2, A. sp. 3) were first reported from the South China Sea. And one cyst type(Cochlodinium sp. ) was first reported in the world. Scrippsiella trochoidea is the dominant species inthis area, accounting for 45 % of all the cysts. There are 2 cysts of toxic dinoflagellate (Alexandriumtamarensis and Gymnodinium catenatum ). But there is no relationship between cyst number and grainsize distribution. | Haifeng Gu, Qi Fang, Jun Sun, Dongzhao Lan, Feng Cai, Zhiyong Gao Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005,China. E-mail:Haifenggu@yahoo. comCollege of Marine Life Science, Ocean University of China, Qingdao 266003, China. | 2003 | Acta Oceanologica Sinica2003，22，3： | 4 |
| 17 | Promoting effect of vanadium on catalytic activity of Pt/Ce–Zr–O diesel oxidation catalysts显示文摘A series of Pt–V/Ce–Zr–O diesel oxidation catalysts was prepared using the impregnation method. The catalytic activity and sulfur resistance of Pt–V/Ce–Zr–O were investigated in the presence of simulated diesel exhaust. The effect of vanadium on the structure and redox properties of the catalysts was also investigated using the Brunauer–Emmett–Teller method,X-ray diffraction, H2temperature-programmed reduction, CO temperature-programmed desorption, X-ray photoelectron spectroscopy, and Energy Dispersive Spectroscopy. Results showed that the Pt particles were well dispersed on the Ce–Zr–O carrier through the vanadium isolation effect, which significantly improved the oxidation activity toward CO and hydrocarbons. An electron-withdrawing phenomenon occurred from V to Pt, resulting in an increase in the metallic nature of platinum, which was beneficial to hydrocarbon molecular activation. | Haifeng Huang Bo Jiang Lei Gu Zhonghua Qi Hanfeng Lu | 2015 | Journal of Environmental Sciences2015，27，7： | 3 |
| 18 | In vivo degradation and endothelialization of an iron bioresorbable scaffold显示文摘Detection of in vivo biodegradation is critical for development of next-generation medical devices such as bioresorbable stents or scaffolds(BRSs).In particular,it is urgent to establish a nondestructive approach to examine in vivo degradation of a new-generation coronary stent for interventional treatment based on mammal experiments;otherwise it is not available to semi-quantitatively monitor biodegradation in any clinical trial.Herein,we put forward a semi-quantitative approach to measure degradation of a sirolimus-eluting iron bioresorbable scaffold(IBS)based on optical coherence tomography(OCT)images;this approach was confirmed to be consistent with the present weight-loss measurements,which is,however,a destructive approach.The IBS was fabricated by a metal-polymer composite technique with a polylactide coating on an iron stent.The efficacy as a coronary stent of this new bioresorbable scaffold was compared with that of a permanent metal stent with the name of trade mark Xience,which has been widely used in clinic.The endothelial coverage on IBS was found to be greater than on Xience after implantation in a rabbit model;and our well-designed ultrathin stent exhibited less individual variation.We further examined degradation of the IBSs in both minipig coronary artery and rabbit abdominal aorta models.The present result indicated much faster iron degradation of IBS in the rabbit model than in the porcine model.The semi-quantitative approach to detect biodegradation of IBS and the finding of the species difference might be stimulating for fundamental investigation of biodegradable implants and clinical translation of the next-generation coronary stents. | Wenjiao Lin Hongjie Zhang Wanqian Zhang Haiping Qi Gui Zhang Jie Qian Xin Li Li Qin Haifeng Li Xiang Wang Hong Qiu Xiaoli Shi Wei Zheng Deyuan Zhang Runlin Gao Jiandong Ding | 2021 | Bioactive Materials2021，6，4： | 3 |
| 19 | Fabrication of high performance 3D SiO_2/Si_3N_4 composite via perhydropolysilazane infiltration and pyrolysis显示文摘Perhydropolysilazane, a low viscosity preceramic polymer with good wettability and high char yield, was used to fabricate three-dimensional silica fiber reinforced silicon nitride matrix composites through the repeated infiltration-curing- pyrolysis cycles. With the increase of the pyrolysis temperature from T1, T2 to T3, the density of the composites increased all through, but the flexural strength showed a maximum value at T2 followed by a sharp decrease. The composite prepared at T2 exhibited a good ceramization of the preceramic polymer, a high flexural strength of 144.9 MPa and excellent dielectric property. The high performance of the composite resulted from the good state of the silica fibers, controlled fiber/matrix interfacial microstructures and high-purity dense silicon nitride matrix. | QI Gongjin ZHANG Changrui HU Haifeng CAO Feng WANG Siqing JIANG Yonggang | 2005 | Science China(Technological Sciences)2005，48，6： | 3 |
| 20 | Pilose antler polypeptides ameliorates hypoxic- ischemic encephalopathy by activated neurotrophic factors and SDF1/CXCR4 axis in rats显示文摘Hypoxic-ischemic encephalopathy (催促) 是与高死亡和病态被联系的一个复杂条件。然而,很少有希望的处理为催促存在。在现在的学习,中央目的是识别覆有毛的鹿角多肽(奶头) 的治疗学的效果在上在老鼠催促。Sprague-Dawley (SD ) 老鼠(14 天旧) 被使用并且把组划分了成三包括控制组, hypoxic 局部缺血(嗨) 组和奶头组。在 21 天处理以后,运动活动在对待奶头的老鼠被改进,大脑萎缩被减少,服的浮肿被减轻到某程度。实时量的聚合酶链反应(RT-qPCR ) 分析显示奶头管理减少了在马头鱼尾的怪兽的煽动性的 cytokines 和 apoptosis 基因的表情与相比嗨组。而且,与神经营养的因素有关的基因的 mRNA 表情显著地在马头鱼尾的怪兽被增加。另外,氧化压力标记的表情都在奶头管理以后是下面调整的。而且,奶头起来调整 mRNA 和 SDF1 和 CXCR4 的蛋白质层次,它可以激活 SDF1/CXCR4 轴到节制大脑损害。这些结果建议那个奶头可以潜在地在处理被使用催促。 | Tao Wu Luna Yang Yan Chen Yinhua Ni Jianguo Jiang Wanjing Zhang Qianchen Zhou Xiaojun Zheng Qi Wang Zhengwei Fu Haifeng Li | 2018 | Acta Biochimica et Biophysica Sinica2018，50，3： | 3 |
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