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| 1 | New eolian red clay sequence on the western Chinese Loess Plateau linked to onset of Asian desertification about 25 Ma ago显示文摘The expansion of inland Asia deserts has considerably influenced the environmental, social and economic activities in Asia. Aridification of inland Asia, especially timing of the initiation of Asian desertification, is a contentious topic in paleoclimatology. Late Cenozoic eolian loess-red clay sequences on the Chinese Loess Plateau, which possess abundant paleoclimatic and paleo-environmental information, can be regarded as an indicator of inland Asia desertification. Here we present a detailed magnetostratigraphic investigation of a new red clay sequence about 654 m in Zhuanglang located at the western Chinese Loess Plateau. Sedimentological, geochemical, mineralogical, and quartz morphological lines of evidence show that the red clay is of eolian origin. Magnetostratigraphic correlations indicate that this core sequence spans from 25.6 to 4.8 Ma, and typical eolian red clay appears as early as 25 Ma. This extends the lower limit of the red clay on the Chinese Loess Plateau from the previously thought early Miocene back into the late Oligocene. This new red clay record further implies that the inland Asia desertification was initiated at least by the late Oligocene. This sequence provides a unique high-resolution geological record for understanding the inland Asia desertification process since the late Oligocene. | QIANG XiaoKe AN ZhiSheng SONG YouGui CHANG Hong SUN YouBin LIU WeiGuo AO Hong DONG JiBao FU ChaoFeng WU Feng LU FengYan CAI YanJun ZHOU WeiJian CAO JunJi XU XinWen AI Li | 2011 | Science China Earth Sciences2011，54，1： | 73 |
| 2 | Identification,bioinformatic analysis and expression profiling of candidate mRNA-like non-coding RNAs in Sus scrofa显示文摘Messenger RNA-like non-coding RNAs(mlncRNAs) are a newly identified group of non-coding RNAs(ncRNAs) that may be in-volved in a number of critical cellular events.In this study,93 candidate porcine mlncRNAs were obtained by computational prediction and screening,among which 72 were mapped to the porcine genome.Further analysis of 8 representative candidates revealed that these mlncRNA candidates are not highly conserved among species.Remarkably,one of the candidates,sTF35495,was found to be precursor of a putative porcine microRNA.By RACE PCR,we determined that the full length of sTF35495 was 3 kb.The protein-coding potential of this RNA was tested in silico with no significant finding.Semi-quantitative RT-PCR analysis of the subgroup of 8 candidates revealed two distinct expression profiles and two molecules were further validated by real-time PCR.The predicted pre-microRNA sequence in this study provides a potentially interesting insight into the in vivo function of porcine mlncRNAs and our findings suggest that they play key biological roles in Sus scrofa. | Bang Xiao Xingju Zhang Yong Li Zhonglin Tang Shulin Yang Yulian Mu Wentao Cui Hong Ao Kui Li | 2009 | Journal of Genetics and Genomics2009，36，12： | 7 |
| 3 | Dynamic Expression of MicroRNA-127 During Porcine Prenatal and Postnatal Skeletal Muscle Development显示文摘MicroRNAs(miRNAs), evolutionarily conserved non-coding RNAs in length 21-24 bp, play a critical role in skeletal muscle development. In this study, to explore the function of mircoRNA-127 in porcine skeletal muscle development, eight tissue samples from adult pigs and longissimus muscle samples at 26 developmental stages were collected from Tongcheng and Landrace pigs. The spatial-temporal expression profiles of miRNA-127 were carried out using step-loop quantitative real-time PCR(stemloop RT-PCR). To explore the molecular functions of miRNA-127, we predicted its target genes and performed functional annotation using bioinformatics methods. Results suggested that miRNA-127 was abundantly expressed in heart, ovary, uterus and spleen tissues and was weakly expressed in liver, lung, kidney and small intestine in both Tongcheng and Landrace pigs. And miRNA-127 showed significant expression differences in heart, ovary, spleen and uterus tissues between these two breeds. miRNA-127 basically kept at a relatively stable high level in middle and later embryonic stages and a low expression level in early embryonic stages and postnatal stages, but the expression levels of miRNA-127 were higher in Tongcheng pigs than in Landrace at most developmental stages. miRNA-127 potentially regulated 240 candidate genes. Results of Gene Ontology and KEGG pathway analysis indicated that these genes could be involved in many molecular functions and mechanisms, such as regulation of the force of heart contraction, regulation of transcription, regulation of T cell differentiation, MAPK signaling pathway and GnRH signaling pathway. Many significantly enriched GO terms and KEGG pathways were related to skeletal muscle development. This study will be helpful to understand the biological function for miRNA-127 and identify candidate gene associated with meat production traits in pigs. | YANG Ya-lan LI Yan LIANG Ru-yi ZHOU Rong AO Hong MU Yu-lian YANG Shu-lin LI Kui TANG Zhong-lin | 2014 | Journal of Integrative Agriculture2014，13，6： | 6 |
| 4 | Application of fish index of biological integrity(FIBI) in the Sanmenxia Wetland with water quality implications显示文摘Long-term changes of fish biotic integrity in the Sanmenxia Wetland,North China,since the 1950 s were assessed using the fish index of biological integrity(FIBI). The water and sediment quality was evaluated by the water quality index(WQI) and sediment pollution index(SPI). The results showed that FIBI continuously decreased from 46 to 20 during the past 5 decades,which indicated that the fish community state shifted from fair to very poor conditions,and damming by itself did not affect the fish biotic integrity. At the same time,WQI fell from 83 to 44.1,and SPI increased from 0.99 to 2.14 since the 1960 s,resulting from fast regional socio-economic development and insufficient wastewater treatment. Correlation analysis suggested that water quality significantly affected biotic integrity(r = 0.867,p < 0.05) through direct effects on the fish community. As a representative example of many wetlands in North China,our study clearly demonstrated that the biological integrity was degraded,induced both by water quality deterioration and sediment pollution,further driven by the contradiction between rapid development of regional economy and lagging development of sewage treatment facilities,which were thought to be the main factor responsible for the degradation of biological integrity. | Hong Zhang Baoqing Shan Liang Ao | 2014 | Journal of Environmental Sciences2014，26，8： | 6 |
| 5 | Influence of MoSi_(2) on oxidation protective ability of TaB_(2)-SiC coating in oxygen-containing environments within a broad temperature range显示文摘TaB_(2)-SiC coating modified by different content of MoSi_(2) was fabricated on graphite substrate with SiC inner coating by liquid phase sintering to elevate the anti-oxidation capability of the TaB_(2)-SiC coatings.As compared to the sample with the TaB_(2)-40wt% SiC coating,the coating sample modified with MoSi_(2) exhibited a weight gain trend at lower temperatures,the fastest weight loss rate went down by 76%,and the relative oxygen permeability value reduced from about 1% to near 0.More importantly,the large amount of SiO_(2) glass phase produced over the coating during oxidation was in contact with the modification of MoSi_(2),which was proved to be beneficial to the dispersion of Ta-oxides.A concomitantly formed continuous Ta-Si-O-B compound glass layer showed excellent capacity to prevent oxygen penetration.However,when the TaB_(2) content was sacrificed to increase the MoSi_(2) content,the relative oxygen permeability of the coating increased instead of decreased.Thus,on the basis of ample TaB_(2) content,increasing the MoSi_(2) content of the coating is conducive to reducing the relative oxygen permeability of the coatings in a broad temperature region. | Xuanru REN Junshuai LV Wei LI Yuwen HU Ke SUN Can MA Hong’ao CHU Weiguang WANG Leihua XU Ziyu LI Peizhong FENG | 2020 | Journal of Advanced Ceramics2020，9，6： | 4 |
| 6 | Foliar modifications induced by inhibition of polar transport of auxin显示文摘The effects of auxin polar transport inhibitors, 9-hydroxy-fluorene-9-carboxylic acid (HFCA); 2, 3, 5-triiodobenzoic acid (TIBA) and trans-cinnamic acid (CA)on leaf pattern formation were investigated with shoots formed from cultured leaf explants of tobacco and cultured pedicel explants of Orychophragmus violaceus, and the seedlings of tobacco and Brassica chinensis. Although the effective concentration varies with the inhibitors used, all of the inhibitors induced the formation of trumpet-shaped and/or fused leaves. The frequency of trumpet-shaped leaf formation was related to the concentration of inhibitors in the medium.Histological observation of tobacco seedlings showed that there was only one main vascular bundle and several minor vascular bundles in normal leaves of the control, but there were several vascular bundles of more or less the same size in the trumpet-shaped leaves of treated ones.These results indicated that auxin polar transport played an important role on bilateral symmetry of leaf growth. | NI DI AN LING JIAN WANG ZHI HONG XU ZHEN Ao XIA(Shanghai Institute of Plant Physiology, Chinese Academy of Sciences, Shanghai 200032, China) | 1999 | Cell Research1999，9，1： | 4 |
| 7 | Umbilical Cord Blood Lead Levels in Shanghai, China显示文摘This study was designed to determine the cord blood lead (BPb) levels of babies born in one urban area of Shanghai, and to preliminarily identify the demographic, social environment and prenatal factors which have an effect on the cord BPb concentrations. From August to November 1993, umbilical cord blood samples were obtained from 605 live newborns in the Yangpu Maternal and Child Hospital. 257 samples were excluded from measurement because of clotting. In 348 cord samples, the geometric mean of cord BPb levels was 9. 2μg/dl, with a 95 % confidence interval of the mean 8. 86-9. 54 (μg/dl). 142 babies (40. 8 % ) had cord BPb levels of 10μg/dl or greater. As a result of this high percentage of newborns with BPb levels equal to or greater than 10 μg/dl, we estimate that each year in the Shanghal City about 60,000 newborns are at risk for developing neuropsychological deficiencies caused by maternal lead exposure during pregnancy. To investigate the factors affecting cord blood levels, the subjects with levels greater than the 70th percentile (10. 7μg/dl) (n = 104) and less than the 3oth percentile (7. 4μg/dl) (n = 104) were selected to compare the demographic, environ ment and prenatal medical history. Increased BPb levels at birth were associated with maternal passive smoking, a family member being occupationally exposed to lead, proximity to major traffic way, household coal combustion, neighborhood coal combustion, low level of meternal occupations, and the increasing occurrence of having the high lead foodstuff pidan (preserved duck egg) during pregnancy. We conclude that prenatal lead exposure has become an impor tant health issue for young children in | SHEN XIAO-MING YAN CHONG-HUAI GUO DI WU SHENG-MEI LI REN-QIU HUANG HONG AO LI-MING ZHOU JIAN-DE HONG ZHAO-YI XU JI-DE JIN XING-MING AND TANG JUN-MING (Division of Childhood Lead Poisoning Prevention, Shanghai Institute for Pediatric Rese | 1997 | Biomedical and Environmental Sciences1997，10，1： | 4 |
| 8 | An Outbreak of SARS in a Diabetes Room of a General Hospital without Infected Medical Staff显示文摘Objective To investigate the epidemiologic features of an outbreak of SARS that occurred in a single diabetes room of a general hospital in Beijing in late March 2003.Methods Field investigation was carried out in the ward,the nursing log and the hospitalization medical record of correlative patients were consulted.SARS-CoV in serum specimen from SARS patient was detected by PCR.Results The room where SARS outbreak occurred was on the 13th floor of the 16-story main ward building.There were 6 beds in the room,living with 6 female patients(aged 45-67)who were all hospitalized due to type2 diabetes.On March 24,2003,Patient 1 began to have a fever and cough,chest X-ray showed pneumonia.Five and six days later,Patient 2 and Patient 3 began to have a fever,respectively.Finally,all of these 3 patients died.Their beds were all at the same side of the room,and the other 3 patients at the opposite side were not infected.Serum SARS CoV-RNA of the Patient 3 was positive by nest-PCR.The daughter-in-law of Patient 1who accompanied Patient 1 by the bedside several days,mainly near the window,upwind of Patient 1,was not infected.Medical staff,family members and visitors of the 6 patients were not infected.Conclusions This outbreak was not transmitted by aerosol.The distance droplets travels could be up to 3.43 meters.Droplet spread has direction,and the droplets direction of propagation is closely related with the wind direction and speed.Those at the downwind position of SARS patients were susceptible to be infected.Medical staff wore face masks and good natural ventilation of this ward building may be important reasons for the prevention of infection. | Chun-ling Liu Jun Cheng He Gao Bo Zhang Qun Yuan Ao Tong Yi-an Liang Hong Tang Xiao-hong Guan | 2013 | 国际感染病学(电子版)2013，2，4： | 3 |
| 9 | Maternal intake restriction programs the energy metabolism, clock circadian regulator and m TOR signals in the skeletal muscles of goat offspring probably via the protein kinase A-cAMP-responsive element-binding proteins pathway显示文摘The biological mechanism by which maternal undernutrition increases the metabolic disorder risk of skeletal muscles in offspring is not fully understood.We hypothesize that maternal intake restriction influences metabolic signals in the skeletal muscles of offspring via a glucagon-mediated pathway.Twentyfour pregnant goats were assigned to the control group(100%of the nutrients requirement,n=12)and restricted group(60%of the control feed allowance from pregnant days 45 to 100,n=12).Blood and Longissimus thoracis muscle were sampled from dams(100 d of gestation),fetuses(100 d of gestation),and kids(90 d after birth)in each group.The data were analyzed using the linear MIXED model,with the multiple comparison method of SIDAK applied.Intake restriction reduced(P<0.05)the total blood protein of dams and fetuses.Maternal restriction decreased(P<0.05)the cAMP-responsive element-binding protein 1(CREB1),CREB-binding protein(CREBBP),protein kinase A(PKA),aryl hydrocarbon receptor nuclear translocator-like protein 1(BMAL1),protein kinase B(AKT1),mammalian target of rapamycin(mTOR),and regulatory-associated protein of mTOR(RPTOR)mRNA expression in the fetuses,and reduced(P<0.05)the CREBBP,nuclear receptor subfamily 1 group H member 3(NR1 H3),D-box binding PAR bZIP transcription factor(DBP)and PKA mRNA levels in the kids,but increased(P<0.05)the peroxisome proliferator-activated receptor gamma coactivator 1 alpha(PGC1 A)and tuberous sclerosis 2(TSC2)mRNA levels in the fetuses.The mRNA expression of clock circadian regulator(CLOCK)and TSC2 genes was increased(P<0.05)in the restricted kids.The protein expression of total PKA and phosphorylated PKA in the restricted fetuses and kids were downregulated(P<0.05),and the protein expression of total mTOR and phosphorylated mTOR were reduced(P<0.05)in the restricted fetuses and kids.Maternal intake restriction regulated fat oxidation,protein synthesis,and circadian clock expression in the muscles of the offspring probably via the glucagon-mediated PKA-CREB pathway,which reveals a noteworthy molecular pathway that maternal undernutrition leads to metabolic adaptation of skeletal muscle in offspring. | Xiaoling Zhou Qiongxian Yan Hong Yang Ao Ren Zhixiong He Zhiliang Tan | 2021 | Animal Nutrition2021，，4： | 2 |
| 10 | Exosomes secreted from cardiomyocytes suppress the sensitivity of tumor ferroptosis in ischemic heart failure显示文摘Heart failure(HF)patients in general have a higher risk of developing cancer.Several animal studies have indicated that cardiac remodeling and HF remarkably accelerate tumor progression,highlighting a cause-and-effect relationship between these two disease entities.Targeting ferroptosis,a prevailing form of non-apoptotic cell death,has been considered a promising therapeutic strategy for human cancers.Exosomes critically contribute to proximal and distant organ-organ communications and play crucial roles in regulating diseases in a paracrine manner.However,whether exosomes control the sensitivity of cancer to ferroptosis via regulating the cardiomyocyte-tumor cell crosstalk in ischemic HF has not yet been explored.Here,we demonstrate that myocardial infarction(MI)decreased the sensitivity of cancer cells to the canonical ferroptosis activator erastin or imidazole ketone erastin in a mouse model of xenograft tumor.Post-MI plasma exosomes potently blunted the sensitivity of tumor cells to ferroptosis inducers both in vitro in mouse Lewis lung carcinoma cell line LLC and osteosarcoma cell line K7M2 and in vivo with xenograft tumorigenesis model.The expression of miR-22-3p in cardiomyocytes and plasma-exosomes was significantly upregulated in the failing hearts of mice with chronic MI and of HF patients as well.Incubation of tumor cells with the exosomes isolated from post-MI mouse plasma or overexpression of miR-22-3p alone abrogated erastin-induced ferroptotic cell death in vitro.Cardiomyocyte-enriched miR-22-3p was packaged in exosomes and transferred into tumor cells.Inhibition of cardiomyocyte-specific miR-22-3p by AAV9 sponge increased the sensitivity of cancer cells to ferroptosis.ACSL4,a pro-ferroptotic gene,was experimentally established as a target of miR-22-3p in tumor cells.Taken together,our findings uncovered for the first time that MI suppresses erastin-induced ferroptosis through releasing miR-22-3p-enriched exosomes derived from cardiomyocytes.Therefore,targeting exosome-mediated cardiomyocyte/tumor pathological communication may offer a novel approach for the ferroptosis-based antitumor therapy. | Ye Yuan Zhongting Mei Zhezhe Qu Guanghui Li Shuting Yu Yingqi Liu Kuiwu Liu Zhihua Shen Jiaying Pu Yanquan Wang Changhao Wang Zhiyong Sun Qian Liu Xiaochen Pang Ao Wang Zijing Ren Tong Wang Ying Liu Jinhuan Hong Jiajie Xie Xin Li Zhonghua Wang Weijie Du Baofeng Yang | 2023 | Signal Transduction and Targeted Therapy2023，8，4： | 2 |
| 11 | New fossils of the Early Pleistocene Equus huanghoensis(Equidae,Perissodactyla)from Nihewan in Hebei Province of China显示文摘This paper describes new fossils of Equus huanghoensis from an Early Pleistocene bed in Nihewan, Hebei Province, which confirms the classification of E. huanghoensis by Chinese researchers. The new fossils include a relatively complete male skull and mandible with all upper and lower dentition, a broken female skull with fragment of mandible and a broken Mc III of Equus sp. The fossils were collected from the Yangshuizhan site of Nihewan. The age of the formation is about 1.6 Ma. The new materials verify some classification characteristics based on teeth published in previous descriptions. These characteristics include large teeth size, short protocone, and tilted protoloph and metaloph. We have added to these characteristics of large skull size, a developed protuberantia supramagna, pentagonal nuchal side, weak Pli cabaline, simple enamel plications; a series of new characteristics strikingly different from the other Equus horses. The broken Mc III is similar to Equus qingyangensis from Qingyang, Gansu. | LI YongXiang ZHANG YunXiang SUN BoYang AO Hong XUE XiangXu | 2016 | Science China Earth Sciences2016，59，1： | 2 |
| 12 | NANOG Alleviates the Damage of Human Hair Follicle Mesenchymal Stem Cells Caused by H_2O_2 through Activation of AKT Pathway显示文摘Objective To explore the protective effect of NANOG against hydrogen peroxide(H_2O_2)-induced cell damage in the human hair follicle mesenchymal stem cells(hHF-MSCs). Methods NANOG was expressed from a lentiviral vector, pLVX-IRES-ZsGreen. NANOG hHF-MSCs and vector hHF-MSCs were treated with 400 μmol/L hydrogen peroxide(H_2O_2) for 2 h, the cell survival rate, cell morphology, ROS production, apoptosis and expression of AKT, ERK, and p21 were determined and compared. Results Our results showed that NANOG could activate AKT and upregulate the expression of p-AKT, but not p-ERK. When treated with 400 μmol/L H_2O_2, NANOG hHF-MSCs showed higher cell survival rate, lower ROS production and apoptosis, higher expression of p-AKT, higher ratio of p-AKT/AKT. Conclusion Our results suggest that NANOG could protect hHF-MSCs against cell damage caused by H_2O_2 through activating AKT signaling pathway. | SHI Jia Hong ZUO Kui Yang ZHANG Ying Yao WANG Bo HAN Xing LIAN Ao Bo LIU Jin Yu | 2019 | Biomedical and Environmental Sciences2019，32，4： | 2 |
| 13 | Spatial and temporal distribution of the cloud optical depth over China based on MODIS satellite data during 2003–2016显示文摘The cloud optical depth(COD) is one of the important parameters used to characterize atmospheric clouds. We analyzed the seasonal variations in the COD over East Asia in 2011 using cloud mode data from the AERONET(Aerosol Robotic Network) ground-based observational network. The applicability of the MODIS(Moderate Resolution Imaging Spectroradiometer) COD product was verified and compared with the AERONET cloud mode dataset. There was a good correlation between the AERONET and the MODIS. The spatial and temporal distribution and trends in the COD over China were then analyzed using MODIS satellite data from 2003 to 2016. The seasonal changes in the AERONET data and the time sequence variation of the satellite data suggest that the seasonal variations in the COD are significant. The result shows that the COD first decreases and then increases with the season in northern China, and reaches the maximum in summer and minimum in winter.However, the spatial distribution change is just the opposite in southern China. The spatial variation trend shows the COD in China decreases first with time and gradually increases after 2014. And the trend of COD in the western and central China is consistent with that in China. While the trend of COD shows a continuously increasing over time in northeast China and the Pearl River Delta. | Xiaopan Li Huizheng Che Hong Wang Xiang'ao Xia Quanliang Chen Ke Gui Hujia Zhao Linchang An Yu Zheng Tianze Sun Zhizhong Sheng Chao Liu Xiaoye Zhang | 2019 | Journal of Environmental Sciences2019，31，6： | 1 |
| 14 | The Establishment of Double-Transgenic Mice that Co-Express the appA and MxA Genes Mediated by Type A Spermatogonia In vivo显示文摘Type A spermatogonial stem cells are the only immortal diploid cells in the postnatal animal that undergo self-renewal through the lifetime of an animal and transmit genes to subsequent generations. In this paper, the generation and characterization of double-transgenic mice co-expressing the Escherichia coliappA gene and human MxA gene generated via the in vivo transfection of type A spermatogonial cells were reported for the first time. The dicistronic expression vector pc DNA-app A-MxA(AMP) and ExGen500 transfection reagent were injected into the testicular tissue of 7-d-old male ICR mice. The mice that underwent testismediated gene transfer were mated with wild-type female mice, and the integration and expression of the foreign genes in the offspring were evaluated. Transgenic mice that co-expressedappA and MxA showed a gene integration rate of 8.89%(16/180). The transgenic mice were environmentally friendly, as the amount of phosphorous remaining in the manure was reduced by as much as 11.1% by theappA gene(P<0.05); these animals also exhibited a strong anti-viral phenotype. | BAI Li-jing JU Hui-ming MU Yu-lian YANG Shu-lin REN Hong-yan AO Hong WANG Chu-duan LI Kui | 2014 | Journal of Integrative Agriculture2014，13，12： | 1 |
| 15 | Vegetation - Climate Relationship and Its Application in the Division o( Vegetation Zone in China显示文摘 | FANG Jing - Yun SONG Yong - Chang LIU Hong - Yah PI- AO Shi- Long | 2002 | Aeta Botanica Sinica2002，44，9： | 1 |
| 16 | The effect of flicker noise on the phase noise of opto-electronic oscillator显示文摘 | Hong J Y'ao S X Li Z L | 2014 | Inter- national Journal for Light and Electron Optics2014，125，4： | 1 |
| 17 | Paclitaxel-coated Gianturco-Roubin Ⅱ (GR Ⅱ) stents reduce neointimal hyperplasia in a porcine coronary in-stent restenosis model显示文摘 | Hong MK Komowski R Bramwell O Ragheb AO Leon MB | 2001 | Coron Artery Dis2001，12，： | 1 |
| 18 | Magnetostratigraphic age of the Xiantai Paleolithic site in the Nihewan Basin and implications for early human colonization of Northeast Asia显示文摘 | Chenglong Deng Qi Wei Rixiang Zhu Hongqiang Wang Rui Zhang Hong Ao Liao Chang Yongxin Pan | 2006 | Earth and Planetary Science Letters2006，，1： | 1 |
| 19 | Characterization of Bacillus probiotics availiable for human use 显示文摘 | Duc LH Hong HA Barbosa TM Henriques AO Cutting SM | 2004 | Appl Environ Microbiol2004，70，： | 1 |
| 20 | Effects of different states of sheep fetal fibroblasts as donor cells on the early development in vitro of reconstructed sheep embryos显示文摘To investigate the effects of different states of donor cells on the development of reconstructed sheep embryos, we designed five treatments of donor cells, including cell passage, cell size, serum starvation, colchicine treatment and gene transfection. Results are as follows: (Ⅰ) Compared with 16-18 passage cells, the morula/blastocyst rate of 5-7 passage cells as donor nuclei was significantly higher (17.3% vs. 4.9%, P<0.05), suggesting the advantage of short-time cultured cells in supporting the development of reconstructed embryos. (Ⅱ) The morula/blastocyst rate of reconstructed embryos derived from medium cells (15-25μm) as donor nuclei was higher than that from large cells (25-33μm) and small cells (8-15μm)( 20.0% vs. 8.0%, 9.7%), indicating that reconstructed embryos from medium cells had a greater potentiality to develop into morula/blastocysts than those from small or large ones. (Ⅲ) The morula/blastocyst rate of reconstructed embryos from donor cells of SS (serum starvation) was lower than that from donor cells of NSS (non-serum starvation), but no significant difference was detected between SS and NSS(11.8% vs. 18.6%, P>0.05). (Ⅳ) Fetal fibroblasts treated with 0.05μmol/L colchicine exhibited a higher morula/blastocyst rate of reconstructed embryos than those treated with 0.10 μmol/L colchicine and untreated ones (27.5% vs. 12.1%, 17.1%), however, no significant difference among the three treatments was detected (P>0.05). (Ⅴ) The morula/blastocyst rate of reconstructed embryos from fetal fibroblasts transfected with GFP gene only was 3.1%, significantly lower than that from non-transgenic cells (3.1% vs. 20.4%, P<0.05). In conclusion, our results demonstrated that fetal fibroblasts of fewer passages, medium size could ensure a higher morula/blastocyst rate of reconstructed embryos. Serum starvation of donor cells might be unnecessary to the development of reconstructed embryos. Donor cells treated with 0.05μmol/L colchicine could facilitate the development of reconstructed embryos. Additionally, as cells transfected with GFP gene were used as donor nuclei, adverse effect on the development of reconstructed embryos was observed. Therefore, the developmental efficiency of reconstructed embryos could be improved if proper treatments to donor cells were used. | WANG Hai, AO Hong, PAN QiuZhen, LI RongQi, ZHAO MengBin, LI AN ZhengXing, LI Ning & WU ChangXin 11nstitute of Zoology, Chinese Academy of Sciences, Beijing 100080, China 2 Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100094, China 3 Northeast Agricultural University, Harbin 150030, China 4Beijing Glorious Land Agricultural Co., Ltd. Beijing 100049, China 5 College of Animal Science and Technology, China Agricultural University, Beijing 100094, China 6 State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing 100094, China | 2007 | Science China(Life Sciences)2007，50，2： | 1 |
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