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| 1 | Fabrication of Photo-crosslinked Chitosan- Gelatin Scaffold in Sodium Alginate Hydrogel for Chondrocyte Culture显示文摘 | Feng Liu Ali Goodarzi Haifeng Wang Joanna Stasiak Jianbo Sun Yu Zhou Peng Zhao Cuijun Deng Hongzhen Xu Xing Tang Hailong He Chao Lin Jiansheng Su | 2014 | Bio-Medical Materials and Engineering2014，，1： | 1 |
| 2 | Optimization of Preparation and Brewing Process of Radix Polygonum Multiflorum Preparata-Herba Taxilli Tea Bag by Orthogonal Test Combined with Comprehensive Weighted Score Analysis显示文摘[Objectives]This study aimed to optimize the preparation process of Radix Polygonum Multiflorum Preparata-Herba Taxilli tea bags.[Methods]Taking the comprehensive weighed score of total flavonoid content,soup color,aroma and taste as an indicator,the preparation process of tea bags was optimized using an orthogonal test.[Results]The results of the orthogonal test show that the optimal preparation process for the Radix Polygonum Multiflorum Preparata-Herba Taxilli tea bags are as follows:particle size of 14 meshes,solid to liquid ratio of 1∶40,brewing temperature of 70℃,and brewing time of 10 min.[Conclusions]The optimized preparation process is reasonable and feasible.It is suitable for the production of Radix Polygonum Multiflorum Preparata-Herba Taxilli tea bags. | Shenggao YIN Hongzhen TANG Jinlian LI Yute ZHONG Dongyan LIAO | 2019 | Medicinal Plant2019，10，5： | 1 |
| 3 | Fast Zn^(2+)mobility enabled by sucrose modified Zn^(2+)solvation structure for dendrite-free aqueous zinc battery显示文摘Aqueous zinc battery has been regarded as one of the most promising energy storage systems due to its low cost and environmental benignity.However,the safety concern on Zn anodes caused by uncontrolled Zn dendrite growth in aqueous electrolyte hinders their application.Herein,sucrose with multi-hydroxyl groups has been introduced into aqueous electrolyte to modify Zn^(2+)solvation environment and create a protection layer on Zn anode,thus effectively retarding the growth of zinc dendrites.Atomistic simulations and experiments confirm that sucrose molecules can enter into the solvation sheath of Zn^(2+),and the as-formed unique solvation structure enhances the mobility of Zn^(2+).Such fast Zn^(2+)kinetics in sucrose-modified electrolyte can successfully suppress the dendrite growth.With this sucrose-modified aqueous electrolyte,Zn/Zn symmetric cells present more stable cycle performance than those using pure aqueous electrolyte;Zn/C cells also deliver an impressive higher energy density of 129.7 Wh·kg^(−1)and improved stability,suggesting a great potential application of sucrose-modified electrolytes for future Zn batteries. | Yufang Cao Xiaohui Tang Linge Li Haifeng Tu Yuzhen Hu Yingying Yu Shuang Cheng Hongzhen Lin Liwen Zhang Jiangtao Di Yongyi Zhang Meinan Liu | 2023 | Nano Research2023，16，3： | 0 |
| 4 | Determination of the Content of Astragaloside IV in Yikangshu Granules by High Performance Liquid Chromatography显示文摘[Objectives]The research aimed to establish a high performance liquid chromatography method for the content determination of astragaloside IV in Yikangshu Granules.[Methods]Kromasil 5μm C18(2),100 A,250 mm×4.6 mm was used;the mobile phase was acetonitrile-water(32∶68);flow velocity was 1.0 mL/min;the temperature of evaporator and sprayer was 80 and 30℃;the column temperature was set at 30℃,and injection volume was 20μL.[Results]Astragaloside IV showed a good linear relationship in the range of 1.01-10.14μg with the peak area,and regression equation was lgY=1.7728lgX+1.597(r=0.9999).The limit of detection for astragaloside IV was 1.96 ng,and the average recovery rate was 95.31%.[Conclusions]This method is sensitive,accurate and reproducible,with good linearity,and it is suitable for the content determination of astragaloside IV in health food Yikangshu Granules. | Kun TANG Chundan ZHANG Hongzhen GUO Bing LI | 2022 | Medicinal Plant2022，13，1： | 0 |
| 5 | Preparation of the selenome-thionine derivative of tab-toxin resistance protein显示文摘In order to obtain phase information for the X-ray diffraction of tabtoxin resistance protein (TTR) crystal using the MAD phasing method, a selenomethionine (Se-Met) derivative of TTR was overexpressed in E. coli strain M15, with pQE-30 plasmid, through IPTG induction in M9 medium containing Se-Met. The product was purified to an estimated homogeneity of greater than 95% according to SDS-PAGE, by a Ni-NTA metal affinity followed by a Mono Q anion exchange column chromatography. The successful substitution of Se-Met for methionine (Met) was confirmed by MALDI-TOF and ESI-Quadrupole Mass Spectrometry analysis. The derivative crystal was obtained using similar conditions as those for the native. | HE Hongzhen SHAO Yu CHEN Yuhang LIU Bingbing CAO Zhenbo JIANG Fan LIU Yiwei TANG Hong LIU Jinyuan ZHAO Nanming RAO Zihe | 2001 | Chinese Science Bulletin2001，46，15： | 0 |
| 6 | Determination of the Content of Gastrodin in Jingtianshenma Tablets by High Performance Liquid Chromatography显示文摘[Objectives]The research aimed to establish a high performance liquid chromatography method for the content determination of gastrodin in Jingtianshenma Tablets.[Methods]A phenomenex Luna C18(250 mm×4.6 mm,5μm)column was used;the mobile phase was acetonitrile-0.05%phosphoric acid solution(3∶97);the detection wavelength was 220 nm,and the column temperature was set at 25℃.[Results]Gastrodin showed a good linear relationship in the range of 0.0984-0.5904μg with the peak area,and regression equation was Y=2000000X-51999(r=0.9999).The limits of detection and quantification for gastrodin were 2.50 and 4.20 ng respectively,and the average recovery rate was 95.95%.[Conclusions]This method is sensitive,accurate and reproducible,with good linearity,and it is suitable for the content determination of gastrodin in health food Jingtianshenma Tablets. | Kun TANG Hongzhen GUO Jie QIAO Bing LI | 2021 | Medicinal Plant2021，12，6： | 0 |
| 7 | The Equivalent Parameters of an Inclined Slot in Two Crossed Waveguides with Radiating Slots显示文摘TheEquivalentParametersofanInclinedSlotinTwoCrossedWaveguideswithRadiatingSlots￥LueShanwei&TangHongzhen(BeijingUniversityofAe... | Lue Shanwei & Tang Hongzhen(Beijing University of Aeronautics and Astronautics, Beijing 100083, China) | 1995 | Journal of Systems Engineering and Electronics1995，6，1： | 0 |
| 8 | High-throughput sorting of two-color fluorescent-labeled zebrafish embryos显示文摘The zebrafish embryos were widely employed in genetics,development and drug discovery studies as miniatured animal models.Sorting of two-color fluorescent embryos is often required in large-scale experiments but it is challenging to manually sort with high efficiency.Here,we reported a high-throughput sorting system for two-color fluorescent zebraflsh embryos.The embryos can be automatically loaded from a sample pool and sorted based on the average fluorescent intensity.The two-color fluorescent signals were split into two lines and detected by an area array camera.The system achieves the sorting of 100 embryos in less than 10 min with an accuracy of greater than 95%. | Hongzhen Tang Linbo Wang Xiaohu Chen Chong Chen Hui Li Guang Yang | 2023 | Journal of Innovative Optical Health Sciences2023，16，5： | 0 |
| 9 | Development of a rapid neutralizing antibody test for SARS-CoV-2 and its application for neutralizing antibody screening and vaccinated serum testing显示文摘Background:Since the outbreak of coronavirus disease(COVID-19),the high infection rate and mutation fre-quency of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),the causative agent,have contributed to the ongoing global pandemic.Vaccination has become the most effective means of controlling COVID-19.Tra-ditional neutralizing tests of sera are complex and labor-intensive,therefore,a rapid test for detecting neutralizing antibodies and antibody status post-immunization is needed.Methods:Based on the fact that antibodies exhibit neutralizing activity by blocking the binding of the S protein receptor-binding domain(S-RBD)to ACE2,we developed a rapid neutralizing antibody test,ACE2-Block-ELISA.To evaluate the sensitivity and specificity,we used 54 positive and 84 negative serum samples.We also tested the neutralizing activities of monoclonal antibodies(mAbs)and 214 sera samples from healthy individuals im-munized with the inactivated SARS-CoV-2 vaccine.Results:The sensitivity and specificity of the ACE2-Block ELISA were 96.3%and 100%,respectively.For neu-tralizing mAb screening,ch-2C5 was selected for its ability to block the ACE2-S-RBD interaction.A plaque assay confirmed that ch-2C5 neutralized SARS-CoV-2,with NT 50 values of 4.19,10.63,and 1.074μg/mL against the SARS-CoV-2 original strain,and the Beta and Delta variants,respectively.For the immunized sera samples,the neutralizing positive rate dropped from 82.14%to 32.16%within 4 months post-vaccination.Conclusions:This study developed and validated an ACE2-Block-ELISA to test the neutralizing activities of an-tibodies.As a rapid,inexpensive and easy-to-perform method,this ACE2-Block-ELISA has potential applications in rapid neutralizing mAb screening and SARS-CoV-2 vaccine evaluation. | Yuchang Li Mingyue Wang Hongzhen Wu Hui Zhao Lei Dong Yunfei Li Xiaofeng Li Ying Tang Sen Zhang Jing Li Chengfeng Qin Tao Jiang Yongqiang Deng Xiaoping Kang | 2022 | Infectious Medicine2022，1，2： | 0 |
| 10 | Detection of Volatile Components in Leaves and Bark of Picrasma quassioides by GC-MS显示文摘[Objectives]This study aimed to analyze the volatile oil components of the leaves and bark of Picrasma quassioides.[Methods]The volatile oils in the leaves and bark of P.quassioides were extracted using steam distillation(SD)method and identified with gas chromatography-mass spectrometry(GC-MS).Based on the mass spectrum database(NiST08),combined with artificial spectrum analysis,referring to relevant literature,the base peak and relative abundance were compared to identify the volatile components of the leaves and bark.The relative content of each component was determined by peak area normalization method.[Results]A total of 24 and 23 chemical components were identified in the leaves and bark of P.quassioides,respectively,including 11 common components.[Conclusions]Leaf and bark are different tissues.They contain different volatile chemical components,of which the contents vary greatly.They should be treated differently in the further research and development of P.quassioides resources. | Jingni WANG Yanqiu DENG Hongzhen TANG Hui HUANG Zhenwei ZHANG | 2021 | Medicinal Plant2021，12，1： | 0 |
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