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| 1 | From surface to bulk modification: Plasma polymerization of amine-bearing coating by synergic strategy of biomolecule grafting and nitric oxide loading显示文摘Integration of two or more biomolecules with synergetic and complementary effects on a material surface can help to obtain multi-functions for various biomedical applications.However,the amounts of biomolecules integrated and their physiological functions are compromised due to the limited surface anchoring sites.Herein,we propose a novel concept of film engineering strategy“from surface to bulk synergetic modification”.This new concept is realized by employing the surface amine groups of plasma polymerized allylamine(PPAm)film for grafting a molecule e.g.,thrombin inhibitor,bivalirudin(BVLD),meanwhile its bulk amine groups is used as a universal depot for storing and releasing therapeutic nitric oxide(NO)gas as supplement to the functions of BVLD.It is demonstrated that such a“from surface to bulk synergetic modification”film engineering can impart the modified-substrates with anti-platelet and anti-coagulant dual functions,giving rise to a highly endotheliummimetic thromboresistant property.We believe that our research provides a very promising strategy to deliver multifunctional surface versatilely that require NO release in combination with other properties,which will find broad biomedical applications in blood-contacting devices,and et al.Moreover,it also provides a brand-new film engineering strategy for tailoring surface multi-functionalities of a wide range of materials. | Tong Yang Zeyu Du Hua Qiu Peng Gao Xin Zhao Huaiyu Wang Qiufen Tu Kaiqin Xiong Nan Huang Zhilu Yang | 2020 | Bioactive Materials2020，5，1： | 10 |
| 2 | Endothelium-Mimicking Multifunctional Coating Modified Cardiovascular Stents via a Stepwise Metal-Catechol-(Amine)Surface Engineering Strategy显示文摘Stenting is currently the major therapeutic treatment for cardiovascular diseases.However,the nonbiogenic metal stents are inclined to trigger a cascade of cellular and molecular events including inflammatory response,thrombogenic reactions,smooth muscle cell hyperproliferation accompanied by the delayed arterial healing,and poor reendothelialization,thus leading to restenosis along with late stent thrombosis.To address prevalence critical problems,we present an endothelium-mimicking coating capable of rapid regeneration of a competently functioning new endothelial layer on stents through a stepwise metal(copper)-catechol-(amine)(MCA)surface chemistry strategy,leading to combinatorial endothelium-like functions with glutathione peroxidase-like catalytic activity and surface heparinization.Apart from the stable nitric oxide(NO)generating rate at the physiological level(2:2×10^(-10) mol/cm^(2)/min lasting for 60 days),this proposed strategy could also generate abundant amine groups for allowing a high heparin conjugation efficacy up to∼1μg/cm^(2),which is considerably higher than most of the conventional heparinized surfaces.The resultant coating could create an ideal microenvironment for bringing in enhanced antithrombogenicity,anti-inflammation,anti-proliferation of smooth muscle cells,re-endothelialization by regulating relevant gene expressions,hence preventing restenosis in vivo.We envision that the stepwise MCA coating strategy would facilitate the surface endothelium-mimicking engineering of vascular stents and be therefore helpful in the clinic to reduce complications associated with stenosis. | Ying Yang Peng Gao Juan Wang Qiufen Tu Long Bai Kaiqin Xiong Hua Qiu Xin Zhao Manfred FMaitz Huaiyu Wang Xiangyang Li Qiang Zhao Yin Xiao Nan Huang Zhilu Yang | 2020 | Research2020，，1： | 8 |
| 3 | The gating charge pathway of an epilepsy-associated potassium channel accommodates chemical ligands显示文摘电压门钾(Kv ) 隧道在电压传感器领域(VSD ) 从 gating 费用的运动导出他们的电压敏感。gating 在 VSD 通过一条物理小径控告 translocate 打开或关门隧道。以前的研究证明 gating 控告 Shaker 的小径, Kv1.2-2.1 妄想的隧道被堵塞,形成集中的电场和 gating 费用转移中心的结构的基础。这里,我们证明 gating 控告电压门 KCNQ2 钾隧道的小径,哪个引起癫痫的活动减小,能提供各种各样的小分子 ligands。记录的联合 mutagenesis,分子的模拟和 electrophysiological,为探查使活跃之物的一个有约束力的模型, ztz240,在 gating 费用,小径被定义。这个信息被用来建立指向定义 ligand 有约束力的衣袋的基于船坞的虚拟屏蔽试金。有五新 chemotypes 的九使活跃之物被识别,并且在 vivo,实验显示出那三 ligands 绑定到 gating 费用小径展览重要反癫痫活动。由指向衣袋的虚拟屏蔽的各种各样的新奇使活跃之物的鉴定在 gating 费用小径支持一个 ligand 有约束力的地点的存在。gating 费用小径的能力提供新卓见提供小分子 ligands 进治疗学地相关的 KCNQ2 隧道的 gating 费用小径。 | Ping Li Zhuxi Chen Haiyan Xu Haifeng Sun Hao Li Hong Liu Huaiyu Yang Zhaobing Gao Hualiang Jiang Min Li | 2013 | Cell Research2013，23，9： | 8 |
| 4 | Rapid drift of the Tethyan Himalaya terrane before two-stage India-Asia collision显示文摘The India-Asia collision is an outstanding smoking gun in the study of continental collision dynamics.How and when the continental collision occurred remains a long-standing controversy.Here we present two new paleomagnetic data sets from rocks deposited on the distal part of the Indian passive margin,which indicate that the Tethyan Himalaya terrane was situated at a paleolatitude of∼19.4°S at∼75 Ma and moved rapidly northward to reach a paleolatitude of∼13.7°N at∼61 Ma.This implies that the Tethyan Himalaya terrane rifted from India after∼75 Ma,generating the North India Sea.We document a new two-stage continental collision,first at∼61 Ma between the Lhasa and Tethyan Himalaya terranes,and subsequently at∼53−48 Ma between the Tethyan Himalaya terrane and India,diachronously closing the North India Sea from west to east.Our scenario matches the history of India-Asia convergence rates and reconciles multiple lines of geologic evidence for the collision. | Jie Yuan Zhenyu Yang Chenglong Deng Wout Krijgsman Xiumian Hu Shihu Li Zhongshan Shen Huafeng Qin Wei An Huaiyu He Lin Ding Zhengtang Guo Rixiang Zhu | 2021 | National Science Review2021，8，7： | 7 |
| 5 | Intercalibration of international and domestic ^(40)Ar/^(39)Ar dating standards显示文摘Four international standards, Ga1550, MMhb-1, Lp-6, Bern 4M, and one domestic standard BT-1 have been intercalibrated. The repeated measurements on MMhb-1 with different mass demonstrate that MMhb-1 is inhomogeneous in age and its average age is 519.8 Ma. The results of Bern 4M and Lp-6 reflect that they have an invariable value of 40Ar*/39Ark (F) and the ages we obtained are consensus with their K-Ar age: Lp-6=127.7Ma; Bern 4M=18.2 Ma. Analyses of BT-1 age spectra, Ca/K and Cl/K spectra as well as inverse isochrons indicate that the sample is homogeneous and invariable and keeps close chemically, with its trapped argon isotope composition close to the at- mosphere. The dating results show that age values are reproducible and steady, total fusion age, step-heating age, plateau age and isochron age are in accord with each other within the error range (2σ). Therefore, we recommend 28.7 Ma as the calibrated age of BT-1. We also discuss the variation in neutron flux gradients of Beijing 49-2 reactor. It was found that the neutron flux gradient varies considerably, and more monitors (standard samples) are needed to fix the trend of variation. The coefficient of the 49-2 reactor that transfers the ratio of production rate of 37ArCa/39ArK into Ca/K ratio is 1.78. This is different from that reported earlier, 2.0, which may be caused by the reconstruction of the reactor. | WANG Fei HE Huaiyu ZHU Rixiang SANG Haiqing WANG Yinglan YANG Liekun | 2006 | Science China Earth Sciences2006，49，5： | 6 |
| 6 | Mapping stripe rust resistance genes by BSR-Seq:YrMM58 and YrHY1 on chromosome 2AS in Chinese wheat lines Mengmai 58 and Huaiyang 1 are Yr17显示文摘Stripe rust(yellow rust), caused by Puccinia striiformis f. sp. tritici(PST),is one of the most devastating fungal diseases in common wheat(Triticum aestivum L.) in China and worldwide. Resistance breeding is the most effective strategy to control diseases in crop plants. Chinese wheat lines Mengmai 58 and Huaiyang 1 are highly resistant to PST race CYR34(V26) at the adult plant stage. To genetically map the underlying resistance genes we developed segregating populations by crossing Mengmai 58 and Huaiyang 1 with the susceptible cultivar Nongda 399. The stripe rust resistances in Mengmai 58 and Huaiyang 1 were both controlled by single dominant genes, provisionally designated YrMM58 and YrHY1, respectively. Bulked segregant RNA-Seq(BSR-Seq) analysis showed that YrMM58 and YrHY1 were located in the same distal ~16 Mb region on chromosome 2 AS.Comparative genomics analysis with the physical map of Aegilops tauschii proved useful for developing additional markers to saturate the genetic linkage map. YrMM58 and YrHY1 were mapped to the distal end of chromosome arm 2 AS, with the closest marker WGGB148 being 7.7 cM and 3.8 cM from the resistance gene, which was considered to be Yr17. These markers can be used in marker-assisted selection. | Yong Wang Huaizhi Zhang Jingzhong Xie Bingmin Guo Yongxing Chen Huaiyu Zhang Ping Lu Qiuhong Wu Miaomiao Li Deyun Zhang Guanghao Guo Jian Yang Panpan Zhang Yan Zhang Xicheng Wang Hong Zhao Tingjie Cao Zhiyong Liu | 2018 | The Crop Journal2018，6，1： | 5 |
| 7 | Laser step-heating ^(40)Ar/^(39)Ar dating on young volcanic rocks显示文摘An attempt was made to use CO_2 laser step-heating method to date the late Pleistocene basaltic groundmass (DF-2) from Tengchong volcanic field. Among the fourteen heating steps, ten define a good inverse isochron (MSWD = 1.4) with an age of 32.2 ± 7.1 ka (2 σ).The inverse isochron also shows that the initial argon isotopic ratio is 297.1±2.0 (2σ) which is the same as the atmospheric argon at the 2σ error level. Study indicates that it is a useful means to date young volcanic groundmass with low K content by using CO_2 laser step-heating ^(40)Ar/^(39)Ar method. | WANG Fei HE Huaiyu ZHU Rixiang YANG Liekun SANG Haiqing WANG Yinglan | 2006 | Chinese Science Bulletin2006，51，23： | 4 |
| 8 | Inhibiting Hv1 channel in peripheral sensory neurons attenuates chronic inflammatory pain and opioid side effects显示文摘Both opioids and nonsteroidal anti-inflammatory drugs(NSAIDS)produce deleterious side effects and fail to provide sustained relief in patients with chronic inflammatory pain.Peripheral neuroinflammation(PN)is critical for initiation and development of inflammatory pain.A better understanding of molecular mechanisms underlying PN would facilitate the discovery of new analgesic targets and the development of new therapeutics.Emerging evidence suggests that peripheral sensory neurons are not only responders to painful stimuli,but are also actively engaged in inflammation and immunity,whereas the intrinsic regulatory mechanism is poorly understood.Here we report the expression of proton-selective ion channel Hv1 in peripheral sensory neurons in rodents and humans,which was previously shown as selectively expressed in microglia in mammalian central nervous system.Neuronal Hv1 was up-regulated by PN or depolarizing stimulation,which in turn aggravates inflammation and nociception.Inhibiting neuronal Hv1 genetically or by a newly discovered selective inhibitor YHV98-4 reduced intracellular alkalization and ROS production in inflammatory pain,mitigated the imbalance in downstream SHP-1-pAKT signaling,and also diminished pro-inflammatory chemokine release to alleviate nociception and morphine-induced hyperalgesia and tolerance.Thus,our data reveal neuronal Hv1 as a novel target in analgesia strategy and managing opioids-related side effects. | Qiansen Zhang Yimin Ren Yiqing Mo Peipei Guo Ping Liao Yuncheng Luo Jie Mu Zhuo Chen Yang Zhang Ya Li Linghui Yang Daqing Liao Jie Fu Juwen Shen Wei Huang Xuewen Xu Yanyan Guo Lianghe Mei Yunxia Zuo Jin Liu Huaiyu Yang Ruotian Jiang | 2022 | Cell Research2022，32，5： | 3 |
| 9 | Molecular basis for ligand activation of the human KCNQ2 channel显示文摘The voltage-gated potassium channel KCNQ2 is responsible for M-current in neurons and is an important drug target to treat epilepsy,pain and several other diseases related to neuronal hyper-excitability.A list of synthetic compounds have been developed to directly activate KCNQ2,yet our knowledge of their activation mechanism is limited,due to lack of high-resolution structures.Here,we report cryo-electron microscopy(cryo-EM)structures of the human KCNQ2 determined in apo state and in complex with two activators,ztz240 or retigabine,which activate KCNQ2 through different mechanisms.The activator-bound structures,along with electrophysiology analysis,reveal that ztz240 binds at the voltage-sensing domain and directly stabilizes it at the activated state,whereas retigabine binds at the pore domain and activates the channel by an allosteric modulation.By accurately defining ligand-binding sites,these KCNQ2 structures not only reveal different ligand recognition and activation mechanisms,but also provide a structural basis for drug optimization and design. | Xiaoxiao Li Qiansen Zhangc Peipei Guo Jie Fu Lianghe Mei Dashuai Lv Jiangqin Wang Dongwu Lai Sheng Ye Huaiyu Yang Jiangtao Guo | 2021 | Cell Research2021，31，1： | 3 |
| 10 | Applying high-performance computing in drug discovery and molecular simulation显示文摘In recent decades, high-performance computing(HPC) technologies and supercomputers in China have significantly advanced, resulting in remarkable achievements. Computational drug discovery and design,which is based on HPC and combines pharmaceutical chemistry and computational biology, has become a critical approach in drug research and development and is financially supported by the Chinese government. This approach has yielded a series of new algorithms in drug design, as well as new software and databases. This review mainly focuses on the application of HPC to the fields of drug discovery and molecular simulation at the Chinese Academy of Sciences, including virtual drug screening, molecular dynamics simulation, and protein folding. In addition, the potential future application of HPC in precision medicine is briefly discussed. | Tingting Liu Dong Lu Hao Zhang Mingyue Zheng Huaiyu Yang Yechun Xu Cheng Luo Weiliang Zhu Kunqian Yu Hualiang Jiang | 2016 | National Science Review2016，3，1： | 2 |
| 11 | Conformational sampling on acid-sensing ion channel 1 (ASIC1): implication for a symmetric conformation显示文摘 | Huaiyu Yang Ye Yu Wei-Guang Li Tian-LeXu Hualiang Jiang | 2009 | Cell Research2009，19，8： | 2 |
| 12 | Characteristics of carbonaceous aerosol in the region of Beijing, Tianjin, and Hebei, China显示文摘 | Zhao Pusheng Dong Fan Yang Yadong He Di Zhao Xiujuan Zhang Wenzong Yao Qing Liu Huaiyu | 2013 | Atmospheric Environment2013，，： | 2 |
| 13 | Injectable self-healing hydrogel with siRNA delivery property for sustained STING silencing and enhanced therapy of intervertebral disc degeneration显示文摘Inflammatory responses of nucleus pulposus(NP)can induce imbalanced anabolism and catabolism of extracellular matrix,and the cytosolic dsDNA accumulation and STING-NF-κB pathway activation found in NP inflammation are considered as fairly important cause of intervertebral disc(IVD)degeneration.Herein,we constructed a siSTING delivery hydrogel of aldehyde hyaluronic acid(HA-CHO)and poly(amidoamine)PAMAM/siRNA complex to intervene the abnormal STING signal for IVD degeneration treatment,where the formation of dynamic Schiff base bonds in the system(siSTING@HPgel)was able to overcome the shortcomings such as low cellular uptake,short half-life,and rapid degradation of siRNA-based strategy.PAMAM not only formed complexes with siRNA to promote siRNA transfection,but also served as dynamic crosslinker to construct hydrogel,and the injectable and self-healing hydrogel efficiently and steadily silenced STING expression in NP cells.Finally,the siSTING@HPgel significantly eased IVD inflammation and slowed IVD degeneration by prolonging STING knockdown in puncture-induced IVD degeneration rat model,revealing that STING pathway was a therapeutic target for IVD degeneration and such novel hydrogel had great potential for being applied to many other diseases for gene delivery. | Jiaxin Chen Haifeng Zhu Yutao Zhu Chenchen Zhao Shengyu Wang Yixin Zheng Ziang Xie Yang Jin Honghai Song Linjun Yang Jin Zhang Jiayong Dai Zhijun Hu Huaiyu Wang | 2022 | Bioactive Materials2022，7，3： | 2 |
| 14 | Rational integration of defense and repair synergy on PEEK osteoimplants via biomimetic peptide clicking strategy显示文摘Polyetheretherketone(PEEK)has been widely used as orthopedic and dental materials due to excellent mechanical and physicochemical tolerance.However,its biological inertness,poor osteoinduction,and weak antibacterial activity make the clinical applications in a dilemma.Inspired by the mussel adhesion mechanism,here we reported a biomimetic surface strategy for rational integration and optimization of anti-infectivity and osteo-inductivity onto PEEK surfaces using a mussel foot proteins(Mfps)-mimic peptide with clickable azido terminal.The peptide enables mussel-like adhesion on PEEK biomaterial surfaces,leaving azido groups for the further steps of biofunctionalizations.In this study,antimicrobial peptide(AMP)and osteogenic growth peptide(OGP)were bioorthogonally clicked on the azido-modified PEEK biomaterials to obtain a dual-effect of host defense and tissue repair.Since bioorthogonal clicking allows precise collocation between AMP and OGP through changing their feeding molar ratios,an optimal PEEK surface was finally obtained in this research,which could long-term inhibit bacterial growth,stabilize bone homeostasis and facilitate interfacial bone regeneration.In a word,this upgraded mussel surface strategy proposed in this study is promising for the surface bioengineering of inert medical implants,in particular,achieving rational integration of multiple biofunctions to match clinical requirements. | Meng Li Jiaxiang Bai Huaqiang Tao Li Hao Weiling Yin Xiaoxue Ren Ang Gao Ning Li Miao Wang Shiyuan Fang Yaozeng Xu Liang Chen Huilin Yang Huaiyu Wang Guoqing Pan Dechun Geng | 2022 | Bioactive Materials2022，7，2： | 2 |
| 15 | An investigation of benzimidazole derivative as corrosion inhibitor for mild steel in different concentration HCl solutions显示文摘 | Wang Xiumei Yang Huaiyu Wang Fuhui | 2011 | Corrosion Science2011，53，1： | 1 |
| 16 | Biointerface engineering nanoplatforms for cancer-targeted drug delivery显示文摘Over the past decade,nanoparticle-based therapeutic modalities have become promising strategies in cancer therapy.Selective delivery of anticancer drugs to the lesion sites is critical for elimination of the tumor and an improved prognosis.Innovative design and advanced biointerface engineering have promoted the development of various nanocarriers for optimized drug delivery.Keeping in mind the biological framework of the tumormicroenvironment,biomembrane-camouflaged nanoplatforms have been a research focus,reflecting their superiority in cancer targeting.In this review,we summarize the development of various biomimetic cell membrane-camouflaged nanoplatforms for cancertargeted drug delivery,which are classified according to the membranes fromdifferent cells.The challenges and opportunities of the advanced biointerface engineering drug delivery nanosystems in cancer therapy are discussed. | Huaiyu Zhang Shujun Dong Zhongmin Li Xiangru Feng Weiguo Xu Catrina Mae STulinao Yang Jiang Jianxun Ding | 2020 | Asian Journal of Pharmaceutical Sciences2020，15，4： | 1 |
| 17 | Bio-modificaiton of eucalyptus chemithermo-mechanical pulp with different white-rot fungi显示文摘 | Yang Qifeng Zhan Huaiyu Wang Shuangfei | 2007 | Bioresource2007，2，4： | 1 |
| 18 | Investigation on the inhibition behavior of a pentaerythritol glycoside for carbon steel in 3.5% NaCl saturated Ca(OH) 2 solution显示文摘 | Xin Zhou Huaiyu Yang Fuhui Wang | 2011 | Corrosion Science2011，，： | 1 |
| 19 | BF4 ionic liquids as effective inhibitor for carbon steel in alkaline chloride solution显示文摘 | Xin Zhou Huaiyu Yang Fuhui Wang | 2011 | Electrochimica Acta2011，56，： | 1 |
| 20 | BF4ionic liquids as effective inhibitor for carbon steel in alkaline chloride solution显示文摘 | ZHOU Xin YANG Huaiyu WANG Fuhui | 2011 | Electrochemical Acta2011，56，： | 1 |
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