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| 1 | Activation of mitogen-activated protein kinases in satellite glial cells of the trigeminal ganglion contributes to substance P-mediated inflammatory pain显示文摘Inflammatory orofacial pain,in which substance P (SP) plays an important role,is closely related to the cross-talk between trigeminal ganglion (TG) neurons and satellite glial cells (SGCs).SGC activation is emerging as the key mechanism underlying inflammatory pain through different signalling mechanisms,including glial fibrillary acidic protein (GFAP) activation,phosphorylation of mitogen-activated protein kinase (MAPK) signalling pathways,and cytokine upregulation.However,in the TG,the mechanism underlying SP-mediated orofacial pain generated by SGCs is largely unknown.In this study,we investigated whether SP is involved in inflammatory orofacial pain by upregulating interleukin (IL)-1β and tumour necrosis factor (TNF)-α from SGCs,and we explored whether MAPK signalling pathways mediate the pain process.In the present study,complete Freund’s adjuvant (CFA) was injected into the whisker pad of rats to induce an inflammatory model in vivo.SP was administered to SGC cultures in vitro to confirm the effect of SP.Facial expression analysis showed that pre-injection of L703,606 (an NK-1 receptor antagonist),U0126 (an inhibitor of MAPK/extracellular signal-regulated kinase [ERK] kinase [MEK] 1/2),and SB203580 (an inhibitor of P38) into the TG to induce targeted prevention of the activation of the NK-1 receptor and the phosphorylation of MAPKs significantly suppressed CFA-induced inflammatory allodynia.In addition,SP promoted SGC activation,which was proven by increased GFAP,p-MAPKs,IL-1β and TNF-α in SGCs under inflammatory conditions.Moreover,the increase in IL-1β and TNF-α was suppressed by L703,606,U0126 and SB203580 in vivo and in vitro.These present findings suggested that SP,released from TG neurons,activated SGCs through the ERK1/2 and P38 pathways and promoted the production of IL-1β and TNF-α from SGCs,contributing to inflammatory orofacial pain associated with peripheral sensitization. | Yanyan Zhang Ning Song Fei Liu Jiu Lin Mengke Liu Chaolan Huang Daqing Liao Cheng Zhou Hang Wang Jiefei Shen | 2019 | International Journal of Oral Science2019，11，3： | 6 |
| 2 | Inhibiting Hv1 channel in peripheral sensory neurons attenuates chronic inflammatory pain and opioid side effects显示文摘Both opioids and nonsteroidal anti-inflammatory drugs(NSAIDS)produce deleterious side effects and fail to provide sustained relief in patients with chronic inflammatory pain.Peripheral neuroinflammation(PN)is critical for initiation and development of inflammatory pain.A better understanding of molecular mechanisms underlying PN would facilitate the discovery of new analgesic targets and the development of new therapeutics.Emerging evidence suggests that peripheral sensory neurons are not only responders to painful stimuli,but are also actively engaged in inflammation and immunity,whereas the intrinsic regulatory mechanism is poorly understood.Here we report the expression of proton-selective ion channel Hv1 in peripheral sensory neurons in rodents and humans,which was previously shown as selectively expressed in microglia in mammalian central nervous system.Neuronal Hv1 was up-regulated by PN or depolarizing stimulation,which in turn aggravates inflammation and nociception.Inhibiting neuronal Hv1 genetically or by a newly discovered selective inhibitor YHV98-4 reduced intracellular alkalization and ROS production in inflammatory pain,mitigated the imbalance in downstream SHP-1-pAKT signaling,and also diminished pro-inflammatory chemokine release to alleviate nociception and morphine-induced hyperalgesia and tolerance.Thus,our data reveal neuronal Hv1 as a novel target in analgesia strategy and managing opioids-related side effects. | Qiansen Zhang Yimin Ren Yiqing Mo Peipei Guo Ping Liao Yuncheng Luo Jie Mu Zhuo Chen Yang Zhang Ya Li Linghui Yang Daqing Liao Jie Fu Juwen Shen Wei Huang Xuewen Xu Yanyan Guo Lianghe Mei Yunxia Zuo Jin Liu Huaiyu Yang Ruotian Jiang | 2022 | Cell Research2022，32，5： | 3 |
| 3 | Induction of Wnt signaling antagonists and p21-activated kinase enhances cardiomyocyte proliferation during zebrafish heart regeneration显示文摘Heart regeneration occurs by dedifferentiation and proliferation of pre-existing cardiomyocytes(CMs).However,the signaling mechanisms by which injury induces CM renewal remain incompletely understood.Here,we find that cardiac injury in zebrafish induces expression of the secreted Wnt inhibitors,including Dickkopf 1(Dkkl),Dkk3,secreted Frizzled-related protein 1(sFrpl),and sFrp2,in cardiac tissue adjacent to injury sites.Experimental blocking of Wnt activity via Dkkl overexpression enhances CM proliferation and heart regeneration,whereas ectopic activation of Wnt8 signaling blunts injury-induced CM dedifferentiation and proliferation.Although Wnt signaling is dampened upon injury,the cytoplasmic β-catenin is unexpectedly increased at disarrayed CM sarcomeres in myocardial wound edges.Our analyses indicated that p21-activated kinase 2(Pak2)is induced at regenerating CMs,where it phosphorylates cytoplasmic β-catenin at Ser 675 and increases its stability at disassembled sarcomeres.Myocardial-specific induction of the phospho-mimeticβ-catenin(S675E)enhances CM dedifferentiation and sarcomere disassembly in response to injury.Conversely,inactivation of Pak2 kinase activity reduces the Ser 675-phosphorylatedβ-catenin(pS675-β-catenin)and attenuates CM sarcomere disorganization and dedifferentiation・Taken together,these findings demonstrate that coordination of Wnt signaling inhibition and Pak2/pS675-βYatenin signaling enhances zebrafish heart regeneration by supporting CM dedifferentiation and proliferation. | Xiangwen Peng Kaa Seng Lai Peilu She Junsu Kang Tingting Wang Guobao Li Yating Zhou jianjian Sun Daqing Jin Xiaolei Xu Lujian Liao Jiandong Liu Ethan Lee Kenneth D.Poss Tao P.Zhong | 2021 | Journal of Molecular Cell Biology2021，13，1： | 3 |
| 4 | Inhibitory effects of lidocaine on cerebral vasospasm in a rabbit model of subarachnoid hemorrhage显示文摘Following subarachnoid hemorrhage, vasoconstrictor substances, cellular apoptosis, blood coagulation, and vascular cell proliferation affect the onset of cerebral vasospasm. Previous studies from our laboratory have revealed that injection of lidocaine (2 mg) into the cisterna magna reduces cerebral vasospasm and nerve functional impairment in an animal model of subarachnoid hemorrhage. The present study determined the optimal lidocaine dose for vasospasm and brain injury by injecting different doses of lidocaine into the cisterna magna in a rabbit model of subarachnoid hemorrhage. Results showed that endothelin, tumor necrosis factor-α, and interleukin-6 levels significantly increased in plasma, and calcitonin gene-related peptide levels significantly decreased in plasma (P < 0.05). The number of neurons was decreased, the number of cells expressing c-Fos increased in the hippocampus, and cross-sections and diameters of basilar arteries were reduced (P < 0.05). These changes significantly improved following injection of lidocaine (1, 2, 4, and 6 mg) into the cisterna magna. A dose of 6 mg lidocaine into the cisterna magna resulted in optimal effects on cerebral vasospasm and brain injury following subarachnoid hemorrhage. | Xianqing Shi Baning Ye Yuandong Hu Yuhui Wang Jianquan Li Daqing Liao Jin Liu | 2010 | Neural Regeneration Research2010，5，21： | 2 |
| 5 | Toxicity mechanisms and bioavailability of copper to fish based on an adverse outcome pathway analysis显示文摘Copper(Cu)exists in a variety of forms in different aquatic environments,and affects their bioavailability.In this study we provide a systematic review on toxicity of Cu which focuses on identifying evidence in the mechanisms of Cu toxicity,and apply an adverse outcome pathway(AOP)analysis to identify multiple potential mechanisms and their interactions of Cu toxicity to fish.This analysis process included the mechanisms of behavior toxicant,oxidative toxicant,ion regulation disruption toxicity,as well as endocrine disruption toxicity.It was found that at low levels of Cu exposure,swimming,avoid predators,locating prey and other sensory functions will be impaired,and the organism will suffer from metabolic alkalosis and respiratory acidosis following the inhibition of the carbonic anhydrase active.The main pathway of acute toxicity of Cu to fish is the inhibition of the Na^(+)/K^(+)-ATPase enzyme,and lead to reduced intracellular sodium absorption,as well as Cu-induced increased cell permeability,in turn resulting in increased sodium ion loss,leading to cardiovascular collapse and respiratory insufficiency.The endocrine disruption toxicity of Cu to fish caused growth inhibition and reproductive reduction.In addition,there are several key pathways of Cu toxicity that are affected by hardness(e.g.,Ca^(2+))and intracellular DOC concentrations,including inhibiting Cu-induction,improving branchial gas exchange,altering membrane transport functions,decreasing Na+loss,and increasing Na+uptake.The results of the AOP analysis will provide a robust framework for future directed research on the mechanisms of Cu toxicity. | Wei Liao Ziwei Zhu Chenglian Feng Zhenfei Yan Yajun Hong Daqing Liu Xiaowei Jin | 2023 | Journal of Environmental Sciences2023，，5： | 1 |
| 6 | Comparing mineral weathering and elemental transport between earth's critical zone with different parent rocks in Yanshan Mountain,Hebei province,China显示文摘Bedrock weathering performs a significant influence on the evolution of Earth’s critical zone.Carbonate rock(dolostone),metamorphic rock(gneiss),and sedimentary rock(sandstone)geological formations in Yanshan Mountain,Hebei Province,are taken as objects to probe the controlling of geological formations on weathering characteristics,migration,and enrichment of elements as well as structure of Earth’s critical zone under the identical climate conditions through geological field survey,analysis on minerals component,element distribution in the weathering profile.The dolostone geological formation(DGF)is lithologically dominated by dolostone,characterized by the strongest and predominant chemical weathering.During bedrock weathering and pedogenesis,DGF is marked by significant depletion of CaO,Mg O,S,Mn,Mo and enrichment of N,K,Fe_(2)O_(3),and Zn with concentrations of P,Cu,and B keeping stable.Shortage of soil-forming materials and significant loss are driven by soil erosion,which results in thin regolith and soil.The soil thickness is less than 10 cm,and the regolith thickness is less than 30 cm.The vegetation community is predominantly rock arbor or brush,which is calcivorous and tolerant of barrenness.Plagiogneiss is a dominant rock type of gneiss geological formation(GGF),characterized by the weakest weathering and fast chemical and physical weathering rate.GGF is masked by significant depletion of P,K,CaO,MgO,Fe_(2)O_(3),Mn,Cu and enrichment of N,S,Mo,and B,with contents of Zn keeping stable.Both soil and regolith developed in GGF are relatively thick for one of the reasons that biotite expands during weathering.The soil thickness is more than 50 cm,and the regolith thickness ranges from 100 to 200 cm.The vegetation community is predominantly high-quality economic forests and various arbors because of the enrichment of nutrients in GGF.Sandstone is primarily a rock type of sandstone geological formation(SGF),characterized by moderate weathering degree and slow chemical weathering rate.SGF is marked by significant depletion of P,K,CaO,MgO,Fe_(2)O_(3),and enrichment of N,S,Mn,Cu,Zn,and Mo,with fluctuant changes of Zn and B.The thickness of soil developed in SGF varies between that of DGF and GGF.The soil thickness ranges from 30 to 50 cm,and the regolith thickness ranges from 50 to 100 cm.Chinese pines are widely spread on the shady slopes of SGF.Research provides theoretical support for screening dominant ecological resource areas,ecological industry development and ecological protection and restoration for Yanshan Mountain,Hebei Province. | Zijian Sun Zhen Liao Wei Shen Daqing Fu Xiaofeng Wei Huiqiong Zhang Ziran Chen Lianghui Xiong Tianhao An Hao Wei | 2022 | Acta Geochimica2022，41，6： | 0 |
| 7 | Synthesis and Swelling Behaviors of Microcrystal Muscovite Composite Superabsorbent by Photopolymerization显示文摘A new microcrystal muscovite composite superabsorbent was synthesized by UV photopolymerization using 2-hydroxy-2-methylpropiophenone(1 173) as photoinitiator,N,N-methylene bisacrylamide(MBA) as crosslinker,acrylic acid(AA),acrylamide(AM) and sodium 4-styrenesulfonate(SSS) as comonomers.Factors affecting water and salt absorption of the microcrystal muscovite composite superabsorbent,such as crosslinker amount,microcrystal muscovite concentration,photoinitiator content,and SSS concentration,were systematically studied.Water retention capacity of the composite superabsorbent was also investigated.The results show that microcrystal muscovite composite superabsorbent has water absorbency of 550 g/g,salt absorbency of 62 g/g,and water retention of 60% after heating at 70 ℃ for 40 h.The microcrystal muscovite is physically combined into the polymeric network without destroying its polycrystalline structure and the surface of the microcrystal muscovite composite superabsorbent has some deep and small holes. | 万涛 LIAO Ling HUANG Runqiu WU Daqing Tang Li XIONG Jing CHEN Qiaohe | 2016 | Journal of Wuhan University of Technology(Materials Science)2016，31，1： | 0 |
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