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| 1 | Genome editing with CRISPR/Cas9 in postnatal mice corrects PRKAG2 cardiac syndrome显示文摘PRKAG2 心脏的症候群正染色体的主导的继承疾病在编码 γ 的 PRKAG2 基因从变化被结果;激活安培的蛋白质 kinase 的 2 规章的子单元。影响病人通常开发室的 tachyarrhythmia 并且经历对医疗倔强并且要求心脏的移植的进步的心失败。在这研究,我们与家庭 Wolff-Parkinson-White 症候群从病人在 PRKAG2 识别一个 H530R 变化。由产生转基因的 H530R PRKAG2 和敲门在里面老鼠,我们证明两个模型包括心脏的肥大和肝糖存储概括人的症状,证实 H530R 变化是有原因地与 PRKAG2 有关心脏的症候群。我们进一步联合联系 adeno 的 virus-9 (AAV9 ) 和 CRISPR/Cas9 编辑基因的系统当让野类型的等位基因未经触动时,破坏编码 H530R 的变异的 PRKAG2 等位基因。在出生后的白天的 AAV9-Cas9/sgRNA 的单个全身的注射 4 或白天 42 实质地在转基因的 H530R PRKAG2 和敲门在里面鼠标恢复心的形态学和函数。一起,我们的工作建议在 vivo CRISPR/Cas9,染色体编辑由有选择地破坏引起疾病的变化是在 PRKAG2 心脏的症候群和另外的主导的继承心脏病的治疗的一个有效工具。 | Chang Xie Ya-Ping Zhang Lu Song Jie Luo Wei Qi Jialu Hu Danbo Lu Zhen Yang Jian Zhang Jian Xiao Bin Zhou Jiu-Lin Du Naihe Jing Yong Liu Yan Wang Bo-Liang Li Bao-Liang Song Yan Yan | 2016 | Cell Research2016，26，10： | 12 |
| 2 | Effects of renal sympathetic denervation on post-myocardial infarction cardiac remodeling in rats显示文摘 | Jialu Hu Meng Ji Conway Niu | 2012 | PLo S One2012，7，45： | 1 |
| 3 | Tenglong Buzhong granules(藤龙补中颗粒) inhibits the growth of SW620 human colon cancer in vivo显示文摘OBJECTIVE: To observe the anticancer effects of the granular preparation of Tenglong Buzhong decoction(藤龙补中汤,TBD), i.e Tenglong Buzhong granules(藤龙补中颗粒, TBG), in human SW620 colon cancer. METHODS: BALB/c nude mice were subcutaneously transplanted with SW620 cells, and treated with TBG(2.56 g/kg, once per day) and/or 5-Fu(104 mg/kg, once per week) for 21 d. Apoptosis, Caspase activities and cellular senescence were measured by commercial kits. The protein expression and phosphorylation were detected by Western blot or immunohistochemistry. RESULTS: TBG and 5-Fu inhibited tumor growth. The tumor inhibition rate of the TBG, 5-Fu, and TBG+5-Fu groups was 42.25%, 51.58%, and 76.08%, respectively. Combination of TBG and 5-Fu showed synergetic anticancer effects. TBG and 5-Fu induced apoptosis, activated caspase-3,-8, and-9, increased SMAC expression, inhibited XIAP expression. TBG induced cellular senescence, upregulated cyclin-dependent kinase inhibitor 1a(CDKN1a) and cyclin-dependent kinase inhibitor 2a(CDKN2a) expression, and inhibited phosphorylation of retinoblastoma-associated protein(RB) and expression of cyclin E1(CCNE1) and cyclindependent kinases(CDK) 2. TBG also inhibited angiogenesis accompanied by downregulation of vascular endothelial growth factor(VEGF) and hypoxiainducible factor-1α(HIF-1α). CONCLUSIONS: TBG inhibits SW620 colon cancer growth, induces apoptosis via SMAC-XIAP-Caspases signaling, induces cellular senescence through CDKN1a/CDKN2a-RB-E2F signaling, inhibits angiogenesis by down-regulation of HIF-1α and VEGF, and enhances the effects of 5-Fu. | LI Miao ZHENG Jialu WANG Shuangshuang CHEN Lei PENG Xiao CHEN Jinfang AN Hongmei HU Bing | 2022 | Journal of Traditional Chinese Medicine2022，42，5： | 1 |
| 4 | Centrifugal stress analysis and structure optimization of large and high-speed end milling cutter显示文摘 | Hu Zhanqi Wang Jialu | 2014 | International Journal of Advanced Manufacturing Technology2014，73，14： | 1 |
| 5 | Dedifferentiated human umbilical cord mesenchymal stem cell reprogramming of endogenous hSDF-1a expression participates in neural restoration in hypoxic-ischemic brain damagerats显示文摘The transplantation of human umbilical cord mesenchymal stem cells(hUC-MSCs)can promote hypoxic-ischemic brain damage(HIBD)nerve repair,but finding suitable seed cells to optimize transplantation and improve treatment efficiency is an urgent problem to be solved.In this study,we induced hUC-MSCs into dedifferentiated hUC-MSCs(De-hUC-MSCs),and the morphology,stem cell surface markers,proliferation and tri-directional differentiation ability of the De-hUC-MSCs and hUC-MSCs were detected.A whole-gene chip was utilized for genome cluster,gene ontology and KEGG pathway analyses of differentially expressed genes.De-hUC-MSCs were transplanted into HIBD rats,and behavioral experiments and immunofluorescence assays were used to assess the therapeutic effect.A lentivirus vector for human stromal cell-derived factor-1(hSDF-1a)was constructed,and the role of hSDF-1a in the neuroprotective effect and mechanism of De-hUC-MSCs was verified.De-hUC-MSCs displayed similar cell morphology,stem cell surface marker expression,cell proliferation and even three-dimensional differentiation ability as hUC-MSCs but exhibited greater treatment potential in vivo.The reprogramming mechanism of hSDF-1a participated in the dedifferentiation process.By successfully constructing a stable hSDF-1a cell line,we found that De-hUC-MSCs might participate in nerve repair through the hSDF-1a/CXCR4/PI3K/Akt pathway.De-hUC-MSCs reprogramming of endogenous hSDF-1a expression may mediate the hSDF-1a/CXCR4/PI3K/Akt pathway involved in nerve repair in HIBD rats. | Zhou Xiaoqin Liu Jia Dai Mengjie Gu Jialu Bi Yang Wang Yuting Hu Huajian Liu Bo Zhang Xiaojun Li Zhongyue Chen Jie Li Tingyu Zhan Xue | 2021 | Genes & Diseases2021，8，3： | 1 |
| 6 | Pt autoreduction in L and zeolites显示文摘SINCE Pt supported on non-acidic KL zeolite was found to be a highly active and selective cata-lyst for the aromatization of n-hexane,numerous significant studies have been conducted.Der-ouane et al.attributed the specific behavior of Pt/KL catalyst to the unique channel struc-ture of L zeolite,which might stabilize extremely small Pt clusters.In contrast,Pt | ZHENG Jian, DONG Jialu, XU Qinhua and HU Cheng1. Department of Chemistry, Nanjing University, Nanjing 210093, China 2. Laboratory of Solid State Microstructures, Nanjing University 210093, China | 1997 | Chinese Science Bulletin1997，42，8： | 0 |
| 7 | Effect of Yanggan Jiedu Sanjie formula on human hepatocellular carcinoma Bel-7402 cells显示文摘OBJECTIVE: To observe the effect of Yanggan Jiedu Sanjie(YGJDSJ) formula on human hepatocellular carcinoma Bel-7402 cells.METHODS: Bel-7402 cells were treated with YGJDSJ. Cell proliferation was detected by cell counting kit-8 assay. Cell apoptosis was identified by Hoechst 33258 staining and flow cytometric analysis. Cell cycle distribution was quantified by flow cytometric analysis. Caspase activities were measured by commercial kit. Cell senescence was detected by senescence-associated β-galactosidase(SA-β-gal)staining. Protein expression and phosphorylation were identified by Western blot. Protein expression was knocked-down by siRNA.RESULTS: YGJDSJ inhibited proliferation of Bel-7402 cells in a dose-and time-dependent manner.YGJDSJ induced apoptosis and activated caspase-3, 8, and 9 in Bel-7402 cells. YGJDSJ-induced apoptosis was completely abrogated by a pan caspase inhibitor, Z-VAD-FMK. YGJDSJ also induced cell senescence, up-regulated cyclin-dependent kinase inhibitor 1 a(CDKN1 a) and CDKN2 a expression and down-regulated retinoblastoma protein(RB) phosphorylation in Bel-7402 cells. Specific knockdown of CDKN1 a and CDKN2 a significantly reduced YGJDSJ-induce cell senescence in Bel-7402 cells.CONCLUSION: YGJDSJ inhibited cell proliferation,induced caspase-dependent apoptosis and CDKN1 a/CDKN2 a-RB signalling mediated cell senescence in Bel-7402 cells. Our findings suggest that YGJDSJ might be potential for hepatocellular carcinoma treatment. | Hu Bing Zhang Tong An Hongmei Zheng Jialu Yan Xia Huang Xiaowei Tian Jianhui Li Miao | 2019 | Journal of Traditional Chinese Medicine2019，39，1： | 0 |
| 8 | The role of surface charges in the blinking mechanisms and quantum-confined Stark effect of single colloidal quantum dots显示文摘The two frequently observed phenomena,photoluminescence(PL)blinking and quantum-confined Stark effect(QCSE)-induced spectral diffusion,are not conducive to the applications of colloidal quantum dots(QDs).It remains elusive how these two phenomena are linked to each other.Unraveling the potential link between blinking and QCSE could facilitate the adoption of appropriate strategies that can simultaneously suppress both PL blinking and spectral diffusion.In this work,we investigated the blinking mechanism and QCSE of single CdSe/CdS/ZnS QDs in the presence of positive and negative surface charges using single-dot PL spectroscopy.We found that the negative surface charges can simultaneously suppress PL blinking and spectral diffusion of single QDs.On the other hand,the positive surface charges could change the blinking mechanisms of QDs from Auger-blinking to band-edge carrier(BC)-blinking.Two types of QCSE were observed,and a significant QCSE-induced spectral broadening of 5.25 nm was measured,which could be attributed to the hopping of surface charges between different surface-trap sites.Based on these findings,several theoretical models are proposed to explain various phenomena observed. | Jialu Li Dengfeng Wang Guofeng Zhang Changgang Yang Wenli Guo Xue Han Xiuqing Bai Ruiyun Chen Chengbing Qin Jianyong Hu Liantuan Xiao Suotang Jia | 2022 | Nano Research2022，15，8： | 0 |
| 9 | Non-small cell lung cancers(NSCLCs)oncolysis using coxsackievirus B5 and synergistic DNA-damage response inhibitors显示文摘With the continuous in-depth study of the interaction mechanism between viruses and hosts,the virus has become a promising tool in cancer treatment.In fact,many oncolytic viruses with selectivity and effectiveness have been used in cancer therapy.Human enterovirus is one of the most convenient sources to generate oncolytic viruses,however,the high seroprevalence of some enteroviruses limits its application which urges to exploit more oncolytic enteroviruses. | Bopei Cui Lifang Song Qian Wang Kelei Li Qian He Xing Wu Fan Gao Mingchen Liu Chaoqiang An Qiushuang Gao Chaoying Hu Xiaotian Hao Fangyu Dong Jiuyue Zhou Dong Liu Ziyang Song Xujia Yan Jialu Zhang Yu Bai Qunying Mao Xiaoming Yang Zhenglun Liang | 2023 | Signal Transduction and Targeted Therapy2023，8，10： | 0 |
| 10 | p.N1380del mutation in the pore-forming region of SCN5A gene is associated with cardiac conduction disturbance and ventricular tachycardia显示文摘心脏的钠隧道在 cardiomyocytes 在推动传导的快去极和维护起一个关键作用。SCN5A 基因的变化能导致心律不齐的许多类型。有突然的意外夜的死亡的家庭父亲的历史的一个 14 岁的男孩与周期性的中略进入医院。心脏的 channelopathy 被怀疑,一条病原的离子隧道被在找变化鉴定。proband 表明了湾穴节点机能障碍,室的心悸亢进,包含心房与心室的节点的心脏的传导骚乱和他的捆。proband 和他的母亲收到了定序的整个 exome。exon 上的异质接合的在里面框架删除 N1380del SCN5A 基因在领域 III (S5-S6 ) 在高度保存的毛孔残余定位中的 23 个在 proband 被揭示。变化被定序的 Sanger 在另外的家庭成员估计。probands 生活叔叔和二姐妹是有心脏的传导骚乱的不同的度的无征状的变化搬运人。功能的分析用整个房间的补丁与野类型或变异的隧道在 HEK293T 房间 transfected 夹钳被进行。有 plasmid pcDNA3.1-N1380del-SCN5A 的 HEK293T 房间 transfected 没有可检测的钠水流。总的来说, SCN5A 基因的 N1380del 变化导致钠隧道的功能的损失。N1380del 是能引起心脏的传导缺点和室的心悸亢进的一个病原的变化。 | Zhen Yang Danbo Lu Lei Zhang Jialu Hu Zhenning Nie Chang Xie Fang Qiu Hua Cheng Yan Yan | 2017 | Acta Biochimica et Biophysica Sinica2017，49，3： | 0 |
| 11 | Microglial TRPV1 in epilepsy:Is it druggable for new antiepileptic treatment?显示文摘Epilepsy is one of the most common neurological diseases worldwide with a high prevalence and unknown pathogenesis.Further,its control is challenging.It is generally accepted that an imbalance between the excitatory and inhibitory properties of the central nervous system(CNS)leads to a large number of abnormally synchronized neuronal discharges in the brain.Transient receptor potential vanilloid protein type 1(TRPV1)is a non-selective cation channel that contributes to the regulation of the nervous system and influences the excitability of the nervous system.This includes the release of neurotransmitters,action potential generation due to alterations in ion channels,synaptic transmission,and the changes in glial cells.There is abundant evidence that TRPV1 is widely expressed in the central nervous system(including microglia)and is involved in the development of epilepsy through neuroinflammation.In conclusion,microglial TRPV1 participates in neuroinflammatory reactions and functions as a potential proinflammatory mediator.This presents a novel treatment approach to regulate seizures brought on by neuroinflammation. | JIAO HU JIALU MO XIANGLIN CHENG | 2023 | BIOCELL2023，47，8： | 0 |
| 12 | A comparative study of F_0 patterns between Chinese and other languages显示文摘The fundamental frequency F0 patterns of Chinese were compared with that of English, Japanese, and German at both macro (passage) and micro (fundamental period) levels by using a Laryngograph in this paper. 14 Chinese and 14 foreigners were selected as speakers. The results show that: 1 .The 90% pitch range of Chinese is somewhat larger than that of other languages studied in Hz but it is near the same for all languages in relative scale logF0, 2. The average signed jitter factor in connected speech is neariy the same, about 3.2%, for both male and female speakers and for dif ferent languages studied; 3. The (-)jitter facior is highcr than (+) jitter factor in connected speech; 4. The rate of F0 fluctuation (peak and valley) for Chinese is higher than that for English; 5.The speech rated in syllables per second of Chinese speakers is lower than that of foreign speakers. | ZHANG Jialu and HU Xianghui(Institute of Acoustics. Academia Sinica, Beijing 100080) | 1994 | Chinese Journal of Acoustics1994，13，4： | 0 |
| 13 | Microscopic morphology evolution of the crystal structure of tetrahydrofuran hydrate under flowing condition显示文摘The evolution of the hydrate particle structure during growth and agglomeration under flowing condition affects the particle as well as flow characteristic,which plays an important role in the flow assurance as well as heat transfer in refrigeration systems.Therefore,this article conducts experiments to study and observe the growth and agglomeration process in the main forming stage of hydrate.It was found that the growth of tetrahydrofuran hydrate was anisotropic and in a layered growth pattern.Single crystals generally transformed from octahedral structure to octahedral skeleton structure with growth,however some single crystals also deformed into plate type particles.The thickness of the plate type particles increased gradually during growth,and the edge part increased earlier than the middle part.During agglomeration,the hydrate particles contacted and sintered together.Sand as the impurity didn’t serve as the nucleation center but affected the agglomeration of hydrate particles by collisions.In addition,the effect increased as the sand size decreased.Finally,a microstructure model for hydrate growth and agglomeration was proposed,which showed the hydrate structure evolution in these processes and could lay a foundation for studying the flow assurance of hydrate slurry. | Jialu Zhang Xiang Liu Shuai Liu Yuxing Li Qihui Hu Wuchang Wang | 2022 | Chinese Journal of Chemical Engineering2022，35，5： | 0 |
| 14 | A Review on the Security of the Ethereum-Based DeFi Ecosystem显示文摘Decentralized finance(DeFi)is a general term for a series of financial products and services.It is based on blockchain technology and has attracted people’s attention because of its open,transparent,and intermediary free.Among them,the DeFi ecosystem based on Ethereum-based blockchains attracts the most attention.However,the current decentralized financial system built on the Ethereum architecture has been exposed to many smart contract vulnerabilities during the last few years.Herein,we believe it is time to improve the understanding of the prevailing Ethereum-based DeFi ecosystem security issues.To that end,we investigate the Ethereum-based DeFi security issues:1)inherited from the real-world financial system,which can be solved by macro-control;2)induced by the problems of blockchain architecture,which require a better blockchain platform;3)caused by DeFi invented applications,which should be focused on during the project development.Based on that,we further discuss the current solutions and potential directions ofDeFi security.According to our research,we could provide a comprehensive vision to the research community for the improvement of Ethereum-basedDeFi ecosystem security. | Yue Xue Dunqiu Fan Shen Su Jialu Fu Ning Hu Wenmao Liu Zhihong Tian | 2024 | Computer Modeling in Engineering & Sciences2024，139，4： | 0 |
| 15 | Synergistic combination of Pd nanosheets and porous Bi(OH)_(3) boosts activity and durability for ethanol oxidation reaction显示文摘Highly active and durable Pd-based electrocatalysts for ethanol oxidation reaction(EOR)play a crucial role in the commercialization of direct ethanol fuel cells(DEFCs).However,the poisonous intermediates(especially adsorbed CO species(COad))formed during the EOR process can easily adsorb and block the active sites on Pd electrodes,which in turn limits the catalytic efficiency.Hence,we present a series of Pd-based composites with a strong coupling interface consisting of Pd nanosheets and amorphous Bi(OH)_(3)species.The incorporation of Bi(OH)3 can induce an electron-rich state adjacent to the Pd sites and effectively separate the Pd ensemble,leading to excellent CO tolerance.The optimal Pd-Bi(OH)_(3)NSs catalyst manifests a mass activity of 2.2 A·mgPd^(-1),which is 5.7 and 2.0 times higher than that of Pd NSs and commercial Pd/C catalyst,respectively.Further CO-stripping experiments and CO-DRIFTS tests confirm the excellent CO tolerance on Pd-Bi(OH)3 NSs electrode,leading to the enhanced EOR durability. | Mingyu Chu Jialu Huang Jin Gong Yi Qu Guoling Chen Hu Yang Xuchun Wang Qixuan Zhong Chengwei Deng Muhan Cao Jinxing Chen Xiaolei Yuan Qiao Zhang | 2022 | Nano Research2022，15，5： | 0 |
| 16 | Huge permittivity and premature metallicity in Bi2O2Se single crystals显示文摘Bi_(2)O_(2) Se is a promising material for next-generation semiconducting electronics.It exhibits premature metallicity on the introduction of a tiny amount of electrons,the physics behind which remains elusive.Here we report on transport and dielectric measurements in Bi_(2)O_(2) Se single crystals at various carrier densities.The temperature-dependent resistivity(p)indicates a smooth evolution from the semiconducting to the metallic state.The critical concentration for the metal-insulator transition(MIT)to occur is extraordinarily low(nc~10^(16) cm^(-3)).The relative permittivity of the insulating sample is huge(∈r≈155(10))and varies slowly with temperature.Combined with the light effective mass,a long effective Bohr radius(a_(B)^(*)≈36(2)nm)is derived,which provides a reasonable interpretation of the metallic prematurity according to Mott’s criterion for MITs.The high electron mobility(μ)at low temperatures may result from the screening of ionized scattering centers due to the huge∈r.Our findings shed light on the electron dynamics in two dimensional(2D)Bi_(2)O_(2) Se devices. | ZhuoKai Xu JiaLu Wang Tao Wang WangHua Hu XiaoHui Yang Xiao Lin | 2021 | Science China(Physics,Mechanics & Astronomy)2021，64，6： | 0 |
| 17 | Fundamental frequency patterns of Chinesein different speech modes显示文摘The fundamental frequency F0 patterns of standard Chinese were examined at both macro (passage) level and micro (period) level in different speech modes using a Laryngograph. The results show that: 1. The more speech effort the speakcr makes the higher are the mean F0, mid F0, and modal F0,in connected speech, and at the samc time the pitch range is expanded; 2. The values of mean F0, mid F0, and modal F0, are different in different speech styles (isolated syllables, isolatcd sentences, and connected speech); 3. The speech rate has apparent influence on golttal waves but no significant innuence on the F0 distribution function; and 4. The closed phase is roughly inverse of F0 in isolated syllables with tones. | ZHANG Jialu and HU Xinghui(Institute of Acoustics, Academia Sinica, Beijing 100080) | 1994 | Chinese Journal of Acoustics1994，13，2： | 0 |
| 18 | Highly insulating phase of Bi_(2)O_(2)Se thin films with high electronic performance显示文摘Bi_(2)O_(2)Se is highly competitive as a candidate of next-generation high-performance semiconductors.Though dubbed as semiconductor,Bi_(2)O_(2)Se films exhibited high conductance,i.e.,metallic behavior,due to spontaneously ionized defects.Semiconducting/insulating films are of practical importance in broad applications based on low-power,high-performance electronics,the existence of which lacks firm evidence.Here,we synthesized highly insulating films in a controlled way,which exhibit semiconducting behavior with channel resistance up to 1 TΩ.The electron chemical potential lies within the band gap,in some cases,even below the charge neutrality level,signifying the trace of hole-type semiconducting.The performance of insulating devices remains high,comparable to high-quality devices previously.Especially,the threshold voltage(Vth)is positive,contrary to common negative values reported.Calculations indicate that our synthesis conditions suppress electron donors(Se vacancies(VSe))and promote the formation of compensating acceptors(Bi vacancies(VBi)),leading to insulating behaviors.Our work offers insights into electron dynamics of Bi_(2)O_(2)Se,moves one step further towards p-type transistors and provides a valuable playground for engineering ferroelectricity in high-performance semiconductors. | Tao Wang Zhuokai Xu Ziye Zhu Mengqi Wu Zhefeng Lou Jialu Wang Wanghua Hu Xiaohui Yang Tulai Sun Xiaorui Zheng Wenbin Li Xiao Lin | 2023 | Nano Research2023，16，2： | 0 |
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