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| 1 | Clay minerals in surface sediments of the Pearl River drainage basin and their contribution to the South China Sea显示文摘Clay minerals have played a significant role in the study of the East Asian monsoon evolution in the South China Sea by being able to track oceanic current variations and to reveal contemporaneous pa- leoclimatic changes prevailing in continental source areas.As one of the most important rivers input- ting terrigenous matters to the northern South China Sea,the Pearl River was not previously paid at- tention to from the viewpoint of clay mineralogy.This paper presents a detailed study on clay minerals in surface sediments collected from the Pearl River drainage basin(including all three main channels, various branches,and the Lingdingyang in the estuary)by using the X-ray diffraction(XRD)method. The results indicate that the clay mineral assemblage consists dominantly of kaolinite(35%-65%), lesser abundance of chlorite(20%-35%)and illite(12%-42%),and very scare smectite occurrences (generally<5%).Their respective distribution does not present any obvious difference throughout the Pearl River drainage basin.However,downstream the Pearl River to the northern South China Sea,the clay mineral assemblage varies significantly:kaolinite decreases gradually,smectite and illite increase gradually.Additionally,illite chemistry index steps down and illite crystallinity steps up.These varia- tions indicate the contribution of major kaolinite,lesser illite and chlorite,and very scarce smectite to the northern South China Sea from the Pearl River drainage basin.The maximum contribution of clay minerals from the Pearl River is 72%to the northern margin and only 15%to the northern slope of the South China Sea.In both glacials and interglacials,kaolinite indicates that the ability of mechanical erosion occurred in the Pearl River drainage basin. | LIU ZhiFei Christophe COLIN HUANG Wei CHEN Zhong Alain TRENTESAUX CHEN JianFang | 2007 | Chinese Science Bulletin2007，52，8： | 33 |
| 2 | Human monoclonal antibodies block the binding of SARS-CoV-2 spike protein to angiotensin converting enzyme 2 receptor显示文摘According to the World Health Organization(WHO)newly updated situation report on March 18th,2020,the coronavirus disease 2019(COVID-19)pandemic has confirmed 191,127 cases and claimed 7807 deaths worldwide.1 The etiological agent of COVID-19 has been identified as a novel coronavirus,the severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),belonging to Sarbecovirus subgenus(genus Betacoronavirus,family Coronaviridae)and showing 79.6 and 96.2%sequence identity in nucleotide to SARS-CoV and a bat coronavirus(BatCoV RaTG13),respectively.2–4 Like SARS-CoV infection,a substantial fraction of COVID-19 patients exhibits severe respiratory symptoms and has to be hospitalized in intensive care unit.5–8 Although the mortality rate of COVID-19 is significantly lower than that of SARS-CoV infection,SARS-CoV-2 shows much higher human-to-human transmission rate,rapidly leading to a global pandemic declared by WHO on March 11th,2020. | Xiangyu Chen Ren Li Zhiwei Pan Chunfang Qian Yang Yang Renrong You Jing Zhao Pinghuang Liu Leiqiong Gao Zhirong Li Qizhao Huang Lifan Xu Jianfang Tang Qin Tian Wei Yao Li Hu Xiaofeng Yan Xinyuan Zhou Yuzhang Wu Kai Deng Zheng Zhang Zhaohui Qian Yaokai Chen Lilin Ye | 2020 | Cellular & Molecular Immunology2020，17，6： | 17 |
| 3 | Parkinson’s disease in China:a forty-year growing track of bedside work显示文摘The number and health burden of Parkinson’s disease increase rapidly in China.It is estimated that China will have nearly half of the Parkinson’s disease population in the world in 2030.In this review,we present an overview of epidemiology and health economics status of Parkinson’s disease across China and discuss the risk factors of Parkinson’s disease and related complications.From the view of clinical research,we also discuss the current status of clinical trials,diagnostic biomarkers,treatment of Parkinson’s disease,tertiary network and post-occupation education in Chinese Parkinson’s disease clinics. | Gen Li Jianfang Ma Shishuang Cui Yixi He Qin Xiao Jun Liu Shengdi Chen | 2019 | Translational Neurodegeneration2019，8，1： | 16 |
| 4 | Roles of a sustained activation of NCED3 and the synergistic regulation of ABA biosynthesis and catabolism in ABA signal production in Arabidopsis显示文摘ABA, acting as a stress signal, plays crucial roles in plant resistance to water stress. Because ABA signal production is based on ABA biosynthesis, the regulation of NCED, a key enzyme in the ABA biosynthesis pathway, is normally thought of as the sole factor controlling ABA signal production. Here we demonstrate that ABA catabolism in combination with a synergistic regulation of ABA biosynthesis plays a crucial role in governing ABA signal production. Water stress induced a significant accumulation of ABA, which exhibited different patterns in detached and attached leaves. ABA catabolism followed a temporal trend of exponential decay for both basic and stress ABA, and there was little difference in the catabolic half-lives of basic ABA and stress ABA. Thus, the absolute rate of ABA catabolism, i.e. the amount of ABA catabolized per unit time, increases with increased ABA accumulation. From the dynamic processes of ABA biosynthesis and catabolism, it can be inferred that stress ABA accumulation may be governed by a synergistic regulation of all the steps in the ABA biosynthesis pathway. Moreover, to maintain an elevated level of stress ABA sustained activation of NCED3 should be required. This inference was supported by further findings that the genes encoding major enzymes in the ABA biosynthesis pathway, e.g. NCED3, AAO3 and ABA3 were all activated by water stress, and with ABA accumulation progressing, the expressions of NCED3, AAO3 and ABA3 remained activated. Data on ABA catabolism and gene expression jointly indicate that ABA signal production is controlled by a sustained activation of NCED3 and the synergistic regulation of ABA biosynthesis and catabolism. | REN HuiBo FAN YiJian GAO ZhiHui WEI KaiFa LI GuiFen LIU Jing CHEN Lin LI BingBing HU JianFang JIA WenSuo | 2007 | Chinese Science Bulletin2007，52，4： | 12 |
| 5 | Remodeling the blood–brain barrier microenvironment by natural products for brain tumor therapy显示文摘Brain tumor incidence shows an upward trend in recent years; brain tumors account for 5% of adult tumors, while in children, this figure has increased to 70%. Moreover, 20%–30% of malignant tumors will eventually metastasize into the brain. Both benign and malignant tumors can cause an increase in intracranial pressure and brain tissue compression, leading to central nervous system(CNS) damage which endangers the patients' lives. Despite the many approaches to treating brain tumors and the progress that has been made, only modest gains in survival time of brain tumor patients have been achieved. At present, chemotherapy is the treatment of choice for many cancers, but the special structure of the blood–brain barrier(BBB) limits most chemotherapeutic agents from passing through the BBB and penetrating into tumors in the brain. The BBB microenvironment contains numerous cell types, including endothelial cells, astrocytes, peripheral cells and microglia, and extracellular matrix(ECM). Many chemical components of natural products are reported to regulate the BBB microenvironment near brain tumors and assist in their treatment. This review focuses on the composition and function of the BBB microenvironment under both physiological and pathological conditions, and the current research progress in regulating the BBB microenvironment by natural products to promote the treatment of brain tumors. | Xiao Zhao Rujing Chen Mei Liu Jianfang Feng Jun Chen Kaili Hu | 2017 | Acta Pharmaceutica Sinica B2017，7，5： | 9 |
| 6 | Potential targets for protecting against hippocampal cell apoptosis after transient cerebral ischemiareperfusion injury in aged rats显示文摘Mitochondria play an important role in neuronal apoptosis caused by cerebral ischemia, and the role is mediated by the expression of mitochondrial proteins. This study investigated the involvement of mitochondrial proteins in hippocampal cell apoptosis after transient cerebral ischemia-reperfusion injury in aged rats using a comparative proteomics strategy. Our experimental results show that the aged rat brain is sensitive to ischemia-reperfusion injury and that transient ischemia led to cell apoptosis in the hippocampus and changes in memory and cognition of aged rats. Differential proteomics analysis suggested that this phenomenon may be mediated by mitochondrial proteins associated with energy metabolism and apoptosis in aged rats. This study provides potential drug targets for the treatment of transient cerebral ischemia-reperfusion injury. | Xiangyu Ji Li'na Zhang Ran Liu Yingzhi Liu Jianfang Song He Dong Yanfang Jia Zangong Zhou | 2014 | Neural Regeneration Research2014，9，11： | 9 |
| 7 | Procalcitonin could be a reliable marker in differential diagnosis of post-implantation syndrome and infection after percutaneous endovascular aortic repair显示文摘 | Xue Ling Luo Songyuan Luo Jianfang Liu Zhen Gu Mengnan Kang Huiyuan Yang Fan Nie Bingrong Liu Yuan Huang Wenhui Xie Nianjin He Pengcheng Dong Haojian Ni Zhonghan Fan Ruixin Chen Jiyan | 2014 | Chinese Medical Journal2014，，14： | 8 |
| 8 | CEACAM6 promotes tumor migration, invasion, and metastasis in gastric cancer显示文摘(CEACAM6 ) Carcinoembryonic 抗原相关的房间粘附分子 6 证明在许多 humancancers 的增加的表示,和它的在表示上与提高的移植被联系,侵略,并且在 vivo 转移。这里,我们报导 CEACAM6 在房间衬里的胃的癌症(GC ) 和肿瘤纸巾是起来调整的。在 MKN-45 和 SGC-7901 GC 房间的 Over-expressionof CEACAM6 在 athymic 老鼠在 vitro 和转移支持了移植和侵略,而 MKN-28 和 SNU-16 GC 房间的移植和侵略被 knockdownof CEACAM6 压制。当 CEACAM6 是 over-expressedin SGC-7901 房间时,我们也观察到类固醇受体激活剂(C-SRC ) phosphorylation 被增加。一起拿,这些结果建议 CEACAM6 在 GC 作为 oncoprotein 工作并且可以是 importantmetastatic biomarker 和治疗学的目标。 | Yunqiang Zhang Mingde Zang Jianfang Li Jun Ji Jianian Zhang Xiaolei Liu Ying Qu Liping Su Chen Li Yinyan Yu Zhenggang Zhu Bingya Liu Min yan | 2014 | Acta Biochimica et Biophysica Sinica2014，46，4： | 5 |
| 9 | Dissecting human embryonic skeletal stem cell ontogeny by single-cell transcriptomic and functional analyses显示文摘Human skeletal stem cells(SSCs)have been discovered in fetal and adult long bones.However,the spatiotemporal ontogeny of human embryonic SSCs during early skeletogenesis remains elusive.Here we map the transcriptional landscape of human limb buds and embryonic long bones at single-cell resolution to address this fundamental question.We found remarkable heterogeneity within human limb bud mesenchyme and epithelium,and aligned them along the proximal-distal and anterior-posterior axes using known marker genes.Osteo-chondrogenic progenitors first appeared in the core limb bud mesenchyme,which give rise to multiple populations of stem/progenitor cells in embryonic long bones undergoing endochondral ossification.Importantly,a perichondrial embryonic skeletal stem/progenitor cell(eSSPC)subset was identified,which could self-renew and generate the osteochondral lineage cells,but not adipocytes or hematopoietic stroma.eSSPCs are marked by the adhesion molecule CADM1 and highly enriched with FOXP1/2 transcriptional network.Interestingly,neural crest-derived cells with similar phenotypic markers and transcriptional networks were also found in the sagittal suture of human embryonic calvaria.Taken together,this study revealed the cellular heterogeneity and lineage hierarchy during human embryonic skeletogenesis,and identified distinct skeletal stem/progenitor cells that orchestrate endochondral and intramembranous ossification. | Jian He Jing Yan Jianfang Wang Liangyu Zhao Qian Xin Yang Zeng Yuxi Sun Han Zhang Zhijie Bai Zongcheng Li Yanli Ni Yandong Gong Yunqiao Li Han He Zhilei Bian Yu Lan Chunyu Ma Lihong Bian Heng Zhu Bing Liu Rui Yue | 2021 | Cell Research2021，31，7： | 5 |
| 10 | The histone methyltransferase EZH2 primes the early differentiation of follicular helper T cells during acute viral infection显示文摘Epigenetic modifications to histones dictate the differentiation of naïve CD4^(+) T cells into different subsets of effector T helper(TH)cells.The histone methyltransferase enhancer of zeste homolog 2(EZH2)has been implicated in the mechanism regulating the differentiation of TH1,TH2 and regulatory T(Treg)cells.However,whether and how EZH2 regulates follicular helper T(TFH)cell differentiation remain unknown.Using a mouse model of acute lymphocytic choriomeningitis virus(LCMV)infection,we observed abundant EZH2 expression and associated H3K27me3 modifications preferentially in the early committed virus-specific TFH cells compared to those in TH1 cells.Ablation of EZH2 in LCMV-specific CD4^(+) T cells leads to a selective impairment of early TFH cell fate commitment,but not late TFH differentiation or memory TFH maintenance.Mechanistically,EZH2 specifically stabilizes the chromatin accessibility of a cluster of genes that are important for TFH fate commitment,particularly B cell lymphoma 6(Bcl6),and thus directs TFH cell commitment.Therefore,we identified the chromatin-modifying enzyme EZH2 as a novel regulator of early TFH differentiation during acute viral infection. | Xiangyu Chen Guoshuai Cao Jialin Wu Xinxin Wang Zhiwei Pan Jianbao Gao Qin Tian Lifan Xu Zhirong Li Yaxing Hao Qizhao Huang Pengcheng Wang Minglu Xiao Luoyingzi Xie Shupei Tang Zhenyu Liu Li Hu Jianfang Tang Ran He Li Wang Xinyuan Zhou Yuzhang Wu Mengjie Chen Beicheng Sun Bo Zhu Jun Huang Lilin Ye | 2020 | Cellular & Molecular Immunology2020，17，3： | 4 |
| 11 | Nitrate δ^(15)N and δ^(18)O evidence for active biological transformation in the Changjiang Estuary and the adjacent East China Sea显示文摘The Changjiang Estuary has been considered as one of the most polluted estuaries in the world due to high nitrate (NO-3) input, especially in spring and summer. In this study, δ15N and δ18O of NO-3 , along with other chemical parameters in this area, were measured in spring to evaluate NO-3 biogeochemical processes. A simple two end-members mixing model was used to examine the relative contribution of the Changjiang River Diluted Water and marine water to NO-3 sources in the Changjiang Estuary and the adjacent East China Sea. The isotopic signals show that NO-3 behaved relatively and conservatively in Transect F and Transect P where assimilation was weak possibly due to vertical mixing, while active assimilation and weak nitrification occurred in Transect D. Spatial difference in assimilation was indicated by the~1:1 enrichment of δ15N and δ18O in the three transects, while spatial difference in nitrification was reflected by deviations of δ15 N and δ18O from assimilation line. Our results suggest that the input of the Changjiang River Diluted Water promoted NO-3 assimilation possibly by stratifying the water column which favored the phytoplankton growth. | CHEN Fajin CHEN Jianfang JIA Guodong JIN Haiyan XU Jie YANG Zhi ZHUANG Yanpei LIU Xizhen ZHANG Haisheng | 2013 | Acta Oceanologica Sinica2013，32，4： | 4 |
| 12 | Tribological and economic evaluation of recycled mineral lubricating oils显示文摘Seven kinds of used mineral lubricating oils,including hydraulic oils,film bearing oils,steam turbine oil and gear oils,were recycled by reclamation and adding additives.Physical and chemical properties of the recycled oils were measured and their lubrication performances were evaluated by the original and modified four-ball testers.Worn surfaces were analyzed by energy dispersive X-ray spectroscopy and Raman microscope.The recycling costs of burning,re-refined and refortified were estimated.Results showed that physical and chemical properties of the refortified oils were improved effectively and became in the industrial access standard.With the different friction materials under different loads,refortified oils provided excellent lubrication performances,much better than those of fresh oils.Because of additives replenished,the different lubrication films could form on the worn surfaces.A rough financial calculation revealed that the refortification process could produce the most economic value among the three methods. | LIU JianFang GU KaLi DUAN HaiTao ZHAO Yuan LI Jian | 2013 | Science China(Technological Sciences)2013，56，12： | 4 |
| 13 | Application of long-chain alkenones and U_(37)~k values for paleotemperature estimation in the Arctic Chukchi Sea-Bering Sea area显示文摘Long-chain alkenones were detected in samples of sea surface sediments from the Chukchi Sea and the Bering Sea areas, the Arctic Pole. The analysis result indicates that C37:3 methylketone is predominate in the long-chain alkenones from the Chukchi and Bering Sea sediments. The abundance of C37 to C39 unsaturated alkenones changes in an order of C37 > C38 > C39. Based on C37 /C38 ratio, the detected organism precursors of the long-chain alkenones are mainly coccolithophrid (Emiliania huxleyi). By the calibration relationship between U37k and U37k indices, the sea surface paleotemperature in these seas is estimated. The estimated values of U37k vary from 4.147℃ to 5.706℃ , with a mean value of 5.092℃. | Lu Bing, Chen Ronghua, Wang Zipan, Chen Jianfang, Liu Zhensheng 1. Key Laboratory of Submarine Geosciences, Second Institute of Oceanography, State Oceanic Administration, Hangzhou 310012, China | 2001 | Acta Oceanologica Sinica2001，20，3： | 4 |
| 14 | Motor oil condition evaluation based on on-board diagnostic system显示文摘The condition of the motor oil in civilian cars is difficult to monitor;hence,we propose a method to evaluate the degree of degradation of motor oil using an on‐board diagnostic(OBD)system.Three civilian cars and four motor oils(containing mineral oils and synthetic oils)were subjected to five groups of road tests under urban traffic and high‐way conditions.The operation information,oil service time,mileage,engine operation time,idle time of the engine,and number of start‐ups of the engine were obtained using the proposed OBD system.Physiochemical properties and changes in the components of motor oils during road tests were analyzed in laboratory.The theoretical model of the comprehensive indicators of driving parameters and oil properties were established.The proposed method was successfully applied to different cars,motor oils,and operating conditions in road tests.All the theoretical models had high accuracy and precision.Herein,we provide a method to monitor the oil condition with real‐time driving parameters and provide a reference for end users to change their motor oil reasonably. | Lei WEI Haitao DUAN Dan JIA Yongliang JIN Song CHEN Lian LIU Jianfang LIU Xianming SUN Jian LI | 2020 | Friction2020，8，1： | 3 |
| 15 | Mechanisms of carbon storage and the coupled carbon, nitrogen and sulfur cycles in regional seas in response to global change显示文摘1.Great challenges in scientific frontiers of marine carbon storage in the scenario of global change The marine carbon cycle is influenced by anthropogenic activities,affecting global climate change and casting a significant impact on ecosystems.However,the complex spatiotemporal process of the marine carbon cycle results in the uncertainty in the estimation of marine carbon budget。 | XIE ShuCheng CHEN JianFang WANG FengPing XUN LuYing TANG Kai ZHAI WeiDong LIU JiHua MA WenTao | 2017 | Science China Earth Sciences2017，60，5： | 3 |
| 16 | Pathogenesis and Immunogenicity of an Avian H9N2 Influenza Virus Isolated from Human显示文摘Objective To investigate the pathogenesis and immunogenicity of H9N2 influenza virus A/Guangzhou/333/99 (a reassortant of G1 and G9 viruses isolated from a female patient in 1999) in a mouse model of infection.Methods Mice were infected with increasing virus titers.Viral load in the lungs and trachea was determined by EID50 assay.Pulmonary histopathology was assessed by hematoxylin‐eosin staining.Anti‐HI antibody titers and T‐cell responses to viral HA were determined by ELISPOT and confirmed by flow cytometry.Results Mice presented a mild syndrome after intranasal infection with A/Guangzhou/333/99 (H9N2) influenza virus.Virus was detected in the trachea and lungs of mice harvested on days 3,6,and 9 post‐infection.A T‐cell response to viral HA was detected on day 6 and H9 HA‐specific CD 4+ T‐cells predominated.Seroconversion was detected after 14 days and antibody persisted for at least 28 weeks.Conclusion Our results suggest that H9N2 (A/Guangzhou/333/99) can replicate in the murine respiratory tract without prior adaptation,and both humoral and cell‐mediated immunity play an important role in the immune response. | LIU LiQi Li Zi ZHOU JianFang ZHU Yun DONG Jie ZHAO Xiang GUO JunFeng SHU YueLong | 2011 | Biomedical and Environmental Sciences2011，24，5： | 2 |
| 17 | Bio-Organic Geochemistry research in China: Advances,opportunities and challenges显示文摘The discipline of 'Bio-Organic Geochemistry' is a cross research field between biogeochemistry and traditional organic geochemistry, which focuses on geochemical processes related to the biosynthesis of organic molecules(particularly lipids) by(micro) organisms, organic matter production by primary producers, degradation of organic matter by microbial processes recorded by retainable lipid biomarkers, and organic proxies for studies of paleo-climate, paleo-environments, paleoecology and Earth evolution. This field aims to go beyond the traditional petroleum-oriented Organic Geochemistry by integrating with biogeochemical concepts concerned mostly with biomolecules from cellular material such as DNA and lipids. A formal Chinese organization in Bio-Organic Geochemistry was established in 2012 when the first conference was held in Guangzhou. This organization has witnessed rapid growth over the past six years with focused research addressing organic proxies in paleoclimate and paleoenvironmental applications, with particular rapid development in glycerol dialkyl glycerol tetraethers-derived proxies. Most progresses in China so far are made following or paralleling the international trend in biogeochemical studies. Things have begun to change with China's ambitious initiatives in several bio-geo programs such as the Ocean Deep Drilling Program of China, the Microbial Hydrosphere Program, the Deep Carbon Observatory, and the Microbiome Program. Looking forward in the 21 st Century, the growing Chinese research community in Bio-Organic Geochemistry faces grand opportunities and challenges as Chinese scientists propel themselves toward global research frontiers. | Chuanlun ZHANG Ping'an PENG Meixun ZHAO Shucheng XIE Jianfang CHEN Weiguo LIU | 2018 | Science China Earth Sciences2018，61，12： | 2 |
| 18 | Distribution of Asbestos Enterprises and Asbestosis Cases--China, 1997−2019显示文摘What is already known on this topic?Asbestos is classified as a Class I Carcinogen by the International Agency for Research on Cancer(IARC)because exposure causes mesothelioma and lung cancer in addition to asbestosis and plaques.So far,asbestos has been banned in 67 countries,but chrysotile,a commonly encountered form of asbestos,is still widely used in China and most developing countries.Most asbestos-caused cancers are not reported,recorded,and compensated in many countries.What is added by this report?Enterprises manufacturing asbestos products have been migrating from economically developed Eastern China to relatively underdeveloped central and western regions between 2010 and 2019.Asbestosis cases reported in Tianjin,Beijing,Shandong,Xinjiang,Gansu,Qinghai,and Sichuan accounted for a large proportion of the total cases in China,which was inconsistent with the distribution of asbestos-related enterprises(AREs).The reported asbestosis cases versus total pneumoconiosis cases declined from 2.81%to 0.39%from 2006–2017,and this proportion reached 0.69%in 2018.What are the implications for public health practice?Robust occupational and environmental health assessments and reporting are needed to define the epidemiology of asbestos-related lung diseases,and management of using asbestos and existing products containing asbestos need strengthening and follow-up.Enterprises should be encouraged to use safer substitutes and gradually ban asbestos materials in China. | Mingfeng Chen Huanqiang Wang Jianfang Zhang Chen Yu Weidong Liu Yunhao Xu | 2020 | China CDC weekly2020，2，18： | 2 |
| 19 | FTIR Spectroscopy in Cultural Heritage Studies: Non-destructive Analysis of Chinese Handmade Papers显示文摘Chinese handmade papers have been the carriers of paper-based documents, paintings and calligraphies, which are of great importance for historical and cultural heritage studies. This research developed the attenuated total reflectance-Fourier transform infrared(ATR-FTIR) spectroscopic technique for the analysis of Chinese handmade papers. The test was applied to Chinese handmade papers taken from bark, hemp and bamboo groups, followed by papers from ancient books and arts collected in library and museum. Our study indicated tliat FTIR was an efficient analytical method to identify fiber types of library/museum papers of unknown origin. Moreover, information about degree of crystallinity and state of deterioration of cellulosic fibers was learned from FTIR spectra. This research provides a non-destnictive method to study the molecular structure of cellulosic fibers, and guides tlie selection of appropriate Chinese handmade papers for the restoration of cultural heritage objects. | YAN Yueer WEN Chenqing JIN Ming DUAN Lian ZHANG Ruohong LUO Chan XIAO Jianfang YE Zhaoqi GAO Boxu LIU Peng TANG Yi | 2019 | Chemical Research in Chinese Universities2019，35，4： | 2 |
| 20 | Heterologous expression of Hp BHY and Cr BKT increases heat tolerance in Physcomitrella patens显示文摘Heat stress can restrict plant growth,development,and crop yield.As essential plant antioxidants,carotenoids play significant roles in plant stress resistance.b-carotene hydroxylase(BHY)and b-carotene ketolase(BKT),which catalyze the conversions of b-carotene to zeaxanthin and b-carotene to canthaxanthin,respectively,are key enzymes in the carotenoid biosynthetic pathway,but little is known about their potential functions in stress resistance.Here,we investigated the roles of b-carotene hydroxylase and b-carotene ketolase during heat stress in Physcomitrella patens through expressing a b-carotene ketolase gene from Chlamydomonas reinhardtii(Cr BKT)and a b-carotene hydroxylase gene from Haematococcus pluvialis(Hp BHY)in the moss P.patens.In transgenic moss expressing these genes,carotenoids content increased(especially lutein content),and heat stress tolerance increased,with reduced leafy tissue necrosis.To investigate the mechanism of this heat stress resistance,we measured various physiological indicators and found a lower malondialdehyde level,higher peroxidase and superoxide dismutase activities,and higher endogenous abscisic acid and salicylate content in the transgenic plants in response to high-temperature stress.These results demonstrate that Cr BKT and Hp BHY increase plant heat stress resistance through the antioxidant and damage repair metabolism,which is related to abscisic acid and salicylate signaling. | Jianfang He Ping Li Heqiang Huo Lina Liu Ting Tang Mingxia He Junchao Huang Li Liu | 2019 | Plant Diversity2019，41，4： | 2 |
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