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| 1 | Diagnosis of nervous necrosis virus in orange-spotted grouper,Epinephelus coioides, by a rapid and convenient RT-PCR method显示文摘Viral nervous necrosis(VNN)causes high mortality in marine fish,especially in the grouper,worldwide and in China.Since there is no effective vaccine or drug to deal with VNN,early detection and prevention is important to block its outbreak.In this study,a reverse transcription-polymerase chain reaction(RT-PCR)was developed for the rapid,convenient,and sensitive detection of the VNN pathogen,nervous necrosis virus(NNV),in the grouper.The whole process was completed within 3.5 h from the RNA extraction to PCR product visualization.The detection limit of this method was 200 copies of NNV RNA standard,which corresponded to 200 copies of virus particles.This RT-PCR method was specific to the NNV detection with no cross-reactivity to other fish viral disease pathogens,such as infectious pancreatic necrosis virus(IPNV),infectious hematopoietic necrosis virus(IHNV),spring viraemia of carp virus(SVCV),epizootic haematopoietic necrosis virus(EHNV),and large yellow croaker iridovirus(LYCIV).With this method,the orange-spotted grouper(Epinephelus coioides)fry from hatcheries with or without incidence of the VNN epidemic in Fujian Province were detected.The results showed that all or 93%of the fry from the two hatcheries with incidence of the epidemic were diagnosed as positive,while 40%or 25%of fry from the two hatcheries without the VNN epidemic were also detected as NNV positive,indicating that this RT-PCR method can be used for rapid,sensitive detection of NNV infection and applied in the VNN epidemic alert. | MU Yinnan LIN Kebing CHEN Xinhua AO Jingqun | 2013 | Acta Oceanologica Sinica2013，32，10： | 6 |
| 2 | Effects of Genistein, Apigenin, Quercetin, Rutin and Astilbin on serum uric acid levels and xanthine oxidase activities in normal and hyperuricemic mice显示文摘 | Jingqun Huang Siwang Wang Miaozhang Zhu Jianzong Chen Xiaoxing Zhu | 2011 | Food and Chemical Toxicology2011，，9： | 2 |
| 3 | Kinetics on leaching rare earth from the weathered crust elution-deposited rare earth ores with ammonium sulfate solution显示文摘 | Tian Jun Yin Jingqun Chi Ruan Rao Guohua Jiang Mintao Ouyang Kexian | 2010 | Hydrometallurgy2010，，3： | 2 |
| 4 | On nonreflecting projective characteristic boundary condition and its application in the numerical simulation of asymmetric coronal structures显示文摘The conventional nonreflecting projective charac teristic boundary condition for MHD equations is improved in order to remove spurious oscillations caused by the presence of zero point of the components of magnetic field. The improved boundary condition is easier to be implemented in programming, and its application to the simulation of asymmetric corona can give us stable coronal static structures. | Jingqun Li Fengsi Wei | 2001 | Chinese Science Bulletin2001，46，1： | 1 |
| 5 | Kinetics on leaching rare earth from the weathered crust elution-deposited rare earth ores with ammonium sulfate solution 显示文摘 | Jun T Jingqun Y Ruan C | 2010 | Hydrometallurgy2010，101，34： | 1 |
| 6 | 显示文摘 | Wang Shouren Zhang Jingchun Wang Jingqun | 2001 | Journal of Shandong Institute of Building Materials2001，，： | 1 |
| 7 | Intensified cleaning of organic-fouled reverse osmosis membranes by thermo- responsive polymer (TRP) 显示文摘 | Sanehuan Yu Zhihai Chen Jingqun Liu | 2012 | Journal of Membrane Science2012，37，3： | 1 |
| 8 | Salidroside protects PC12 cells from MPP + -induced apoptosis via activation of the PI3K/Akt pathway显示文摘 | Lingling Zhang Wenjun Ding Huixing Sun Qiong Zhou JingQun Huang Xuefen Li Yonghong Xie Jianzong Chen | 2012 | Food and Chemical Toxicology2012，，8： | 1 |
| 9 | Purification and characterization of a novel antifungal protein secreted by Penicillium chrysogenum from an Arctic sediment显示文摘 | Zhiteng Chen Jingqun Ao Wenchuan Yang Liping Jiao Tianling Zheng Xinhua Chen | 2013 | Applied Microbiology and Biotechnology2013，，24： | 1 |
| 10 | Kinetics on leaching rare earth from the weathered crust elution-deposited rare earth ores with ammonium sulfate solution显示文摘 | Tian Jun Yin Jingqun Chi Ru'an | 2010 | Hydrometallurgy2010，101，34： | 1 |
| 11 | Enhanced leaching process of a low-grade weathered crust elution-deposited rare earth ore with carboxymethyl sesbania gum显示文摘 | Jun Tian Jingqun Yin Xuekun Tang Ji Chen Xianping Luo Guohua Rao | 2013 | Hydrometallurgy2013，，： | 1 |
| 12 | Extraction of rare earths from the leach liquor of the weathered crust elution-deposited rare earth ore with non-precipitation显示文摘 | Tian Jun Yin Jingqun Chen kaihong Rao Guohua Jiang Mintao Chi Ruan | 2010 | International Journal of Mineral Processing2010，，3： | 1 |
| 13 | Enhanced leachability of a lean weathered crust elution-deposited rare-earth ore:effects of sesbania gum filter-aid reagent 显示文摘 | Tian Jun Tang Xuekun Yin Jingqun | 2013 | Metallurgical and Materials2013，44，5： | 1 |
| 14 | Progressive Muscle Relaxation Improves Anxiety and Depression of Pulmonary Arterial Hypertension Patients显示文摘 | Yunping Li Ranran Wang Jingqun Tang | 2015 | Evid Based Complement Ahernat Med2015，11，2： | 1 |
| 15 | Kinetics on leaching rare earth from the weathered crust elution - deposited rare earth ores with ammonium sulfate solu- tion显示文摘 | Tian Jun Yin Jingqun Chi Ruan | 2010 | Hydrometallurgy2010，101，34： | 1 |
| 16 | Drosophila C - type lectins enhance cellular encapsulation 显示文摘 | Jingqun An Erjun Ling Xiao- Qiang Yu | 2007 | Molecular Im- munology2007，44，： | 1 |
| 17 | Cinnamaldehyde reduction of platelet aggregation and thrombosis in rodents 显示文摘 | Jingqun H Siwang W Xiaoxing L | 2007 | Thrombosis Research2007，119，： | 1 |
| 18 | Process optimization on leaching of a lean weathered crust elution-deposited rare earth ores显示文摘 | Jun Tian Xuekun Tang Jingqun Yin Xianping Luo Guohua Rao Mintao Jiang | 2013 | International Journal of Mineral Processing2013，，： | 1 |
| 19 | Optimisation of mass transfer in column elution of rare earths from low grade weathered crust elution-deposited rare earth ore显示文摘 | Tian Jun Yin Jingqun Chen Kaihong Rao Guohua Jiang Mintao Chi Ruan | 2010 | Hydrometallurgy2010，，1： | 1 |
| 20 | Extraction of rare earths from the leach liquor of the weathered crust elution-deposited rare earth ore with non-precipitation显示文摘 | Tian Jun Yin Jingqun Chen kaihong Rao Guohua Jiang Mintao Chi Ruan | 2010 | International Journal of Mineral Processing2010，，3： | 1 |
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