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| 1 | A LuxR family transcriptional regulator AniF promotes the production of anisomycin and its derivatives in Streptomyces hygrospinosus var.beijingensis显示文摘The protein synthesis inhibitor anisomycin features a unique benzylpyrrolidine system and exhibits potent selective activity against pathogenic protozoa and fungi.It is one of the important effective components in Agricultural Antibiotic120,which has been widely used as naturally-originated agents for treatment of crop decay in China.The chemical synthesis of anisomycin has recently been reported,but the complex process with low productivity made the biosynthesis still to be a vital mainstay in efforts.The biosynthetic gene cluster(BGC)of anisomycin in Streptomyces hygrospinosus var.beijingensis has been identified in our previous work,while poor understanding of the regulatory mechanism limited the yield enhancement via regulation engineering of S.hygrospinosus var.beijingensis.In this study here,we characterized AniF as an indispensable LuxR family transcriptional regulator for the activation of anisomycin biosynthesis.The genetic manipulations of aniF and the real-time quantitative PCR(RT-qPCR)revealed that it positively regulated the transcription of the anisomycin BGC.Moreover,the overexpression of aniF contributed to the improvement of the production of anisomycin and its derivatives.Dissection of the mechanism underlying the function of AniF revealed that it directly activated the transcription of the genes aniR-G involved in anisomycin biosynthesis.Especially,one AniF-binding site in the promoter region of aniR was identified by DNase I footprinting assay and an inverted repeat sequence(5′-GGGC-3′)composed of two 4-nt half sites in the protected region was found.Taken together,our systematic study confirmed the positive regulatory role of AniF and might facilitate the future construction of engineering strains with high productivity of anisomycin and its derivatives. | Jufang Shen Lingxin Kong Yan Li Xiaoqing Zheng Qing Wang Weinan Yang Zixin Deng Delin You | 2019 | Synthetic and Systems Biotechnology2019，4，1： | 10 |
| 2 | Medical expenditures for colorectal cancer diagnosis and treatment: A 10-year high-level-hospital-based multicenter retrospective survey in China, 2002-2011显示文摘Objective: Colorectal cancer(CRC) causes a substantial burden of disease in China and the evidence of economic burden triggered is fundamental for priority setting. The aim of this survey was to quantify medical expenditures and the time trends for CRC diagnosis and treatment in China.Methods: From 2012 to 2014, a hospital-based multicenter retrospective survey was conducted in 13 provinces across China. For each eligible CRC patient diagnosed from 2002 to 2011, clinical information and expenditure data were extracted using a uniform questionnaire. All expenditure data were reported in Chinese Yuan(CNY)using 2011 values.Results: Of the 14,536 CRC patients included, the average age at diagnosis was 58.2 years and 15.8% were stageI cases. The average medical expenditure per patient was estimated at 37,902 CNY [95 % confidence interval(95%CI): 37,282-38,522], and the annual average increase rate was 9.2% from 2002 to 2011(P for trend <0.001), with a cumulative increase of 2.4 times(from 23,275 CNY to 56,010 CNY). The expenditure per patient in stages Ⅰ, Ⅱ, Ⅲ and Ⅳ were 31,698 CNY, 37,067 CNY, 38,918 CNY and 42,614 CNY, respectively(P<0.001). Expenditure significantly differed within various subgroups. Expenses for drugs contributed the largest proportion(52.6%).Conclusions: These conservative estimates illustrated that medical expenditures for CRC diagnosis and treatment in tertiary hospitals in China were substantial and increased rapidly over the 10 years, with drugs continually being the main expense by 2011. Relatively, medical expenditures are lower for CRC in the earlier stages. These findings will facilitate the economic evaluation of CRC prevention and control in China. | Jufang Shi Guoxiang Liu Hong Wang Ayan Mao Chengcheng Liu Lanwei Guo Huiyao Huang Jiansong Ren Xianzhen Liao Yana Bai Xiaojie Sun Xinyu Zhu Jialin Wang Bingbing Song Jinyi Zhou Lin Zhu Haike Lei Yuqin Liu Yunyong Liu Lingbin Du Yutong He Kai Zhang Ni Li Wanqing Chen Min Dai Jie He | 2019 | Chinese Journal of Cancer Research2019，31，5： | 8 |
| 3 | No expenditure difference among patients with liver cancer at stageⅠ-ⅣV:Findings from a multicenter cross-sectional study in China显示文摘Objective:The number of liver cancer patients in China accounts for more than half of the world.However,China currently lacks national,multicenter economic burden data,and meanwhile,measuring the differences among different subgroups will be informative to formulate corresponding policies in liver cancer control.Thus,the aim of the study was to measure the economic burden of liver cancer by various subgroups.Methods:A hospital-based,multicenter and cross-sectional survey was conducted during 2012・2014,covering 39 hospitals and 21 project sites in 13 provinces across China.The questionnaire covers clinical information,sociology,expenditure,and related variables.All expenditure data were reported in Chinese Yuan(CNY)using 2014 values.Results:A total of 2,223 liver cancer patients were enrolled,of whom 59.61%were late-stage cases(III-IV),and 53.8%were hepatocellular carcinoma.The average total expenditure per liver cancer patient was estimated as 53,220 CNY,including 48,612 CNY of medical expenditures(91.3%)and 4,608 CNY of non-medical expenditures(8.7%).The average total expenditures in stage I,H,m and stage IV were 52,817 CNY,50,877 CNY,50,678 CNY and 54,089 CNY(P>0.05),respectively.Non-medical expenditures including additional meals,additional nutrition care,transportation,accommodation and hired informal nursing were 1,453 CNY,839 CNY,946 CNY,679 CNY and 200 CNY,respectively.The one-year out-of-pocket expenditure of a newly diagnosed patient was 24,953 CNY,and 77.2%of the patients suffered an unmanageable financial burden.Multivariate analysis showed that overall expenditure differed in almost all subgroups(P<0.05),except for sex,clinical stage,and pathologic type.Conclusions:There was no difference in treatment expenditure for liver cancer patients at different clinical stages,which suggests that maintaining efforts on treatment efficacy improvement is important but not enough.To fiirtherly reduce the overall economic burden from liver cancer,more effort should be given to primary and secondary prevention strategies. | Haike Lei Lin Lei Jufang Shi Yongzhong Wu Ling Liang Huiyao Huang Mei He Fangzhou Bai Maomao Cao Hui Qiu Yuting Wang Chengcheng Liu Jia Du Hong Wang Yan Zhang Mengdi Cao Ji Peng Ni Li Chunfeng Qu Min Dai Wanqing Chen Jie He | 2020 | Chinese Journal of Cancer Research2020，32，4： | 7 |
| 4 | Population-level economic burden of lung cancer in China:Provisional prevalence-based estimations,2017-2030显示文摘Objective:Population-level economic burden is essential for prioritizing healthcare resources and healthcare budget making in the future.However,little is known about the economic burden of lung cancer in China.Methods:A prevalence-based approach was adopted to estimate the economic burden of lung cancer,including direct expenditure(medical and non-medical)and indirect cost(disability and premature death).Data on direct expenditure and work-loss days per patient in each year post-diagnosis were obtained from two primary surveys.Other parameters were obtained from literatures and official reports.Projections were conducted based on varying parameters.All expenditure data were reported in United States dollars(USD)using 2017 value(exchange rate:1 USD=6.760 CNY),with the discount rate of 3%.Results:The total economic burden of lung cancer was estimated to be 25,069 million USD in China in 2017(0.121%of gross domestic productivity,GDP).The estimated direct expenditure was 11,098 million USD,up to1.43%of total healthcare expenditure for China,covering 10,303 million USD and 795 million USD for medical and non-medical expenditure,respectively.The estimated indirect cost was 13,971 million,including 1,517 million USD due to disability and 12,454 million USD due to premature death.Under current assumptions,the projected total economic burden would increase to 30.1 billion USD,40.4 billion USD,and 53.4 billion USD in 2020,2025,and 2030,accounting for 0.121%,0.131%,and 0.146%of China's GDP,respectively.However,if China meets the United Nation sustainable development goal of reducing premature death from non-communicable diseases by one-third by 2030,the total economic burden in 2030 would be 31.9 billion USD,0.087%of China's GDP.Conclusions:The economic burden of lung cancer in China in 2017 is substantial and more likely to increase significantly in the future.Policy makers need to take urgent actions in budget making for health systems.The economic burden could be alleviated by reducing the disease burden of lung cancer via effective control and prevention actions. | Chengcheng Liu Jufang Shi Hong Wang Xinxin Yan Le Wang Jiansong Ren Mark Parascandola Wanqing Chen Alin Dai | 2021 | Chinese Journal of Cancer Research2021，33，1： | 6 |
| 5 | Internalization of NK cells into tumor cells requires ezrin and leads to programmed cell-in-cell death显示文摘细胞毒素的淋巴细胞是在有缺点的房间的有免疫力的反应和消除的组织的关键播放器。我们以前报导了生来的杀手(NK ) 房间进入目标肿瘤房间,导致在肿瘤房间以内的任何一个目标房间死亡或自我毁灭。然而,它留下了至于在成为主观以后的 NK 房间的命运逃犯并且 heterotypic cell-in-cell 过程最近是否与同型的 cell-in-cell 事件的不同,说出 entosis。这里,我们证明 NK 房间在肿瘤房间以内与 apoptosis 的最终的命运经历一个 cell-in-cell 过程并且表明成为主观过程要求肌动朊细胞骨架管理者, ezrin。设想 NK 房间怎么进入肿瘤房间,我们执行了 NK 房间成为主观的即时双颜色成像分析进肿瘤房间。令人惊讶地,大多数 NK 房间在他们的入口以后承诺规划房间死亡进肿瘤房间,它与在同型的 cell-in-cell 过程观察的 entosis 区别地不同。使内在化的 NK 房间的 apoptotic 房间死亡由 caspase 的激活是明显的 3 并且 DNA 破碎。而且,在成为主观以后的 NK 房间死亡被 caspase 禁止者稀释, Z-VAD-FMK,在肿瘤房间以内作为 NK 房间死亡的模式证实 apoptosis。决定为 NK 房间的入口必要的蛋白质因素进肿瘤房间,我们执行了基于 siRNA 的击倒的分析并且在 NK 房间成为主观发现了 ezrin 的一个关键角色。重要地, ezrin 的调停 PKA 的 phosphorylation 支持 NK 房间成为主观过程。我们的调查结果建议 ezrin 由管理 NK 房间成为主观进肿瘤房间的新奇规章的机制。 | Shan Wang Zhen Guo Peng Xia Tingting Liu Jufang Wang Shan Li Lihua Sun Jianxin Lu Qian Wen Mingqian Zhou Li Ma Xia Ding Xiaoning Wang Xuebiao Yao | 2009 | Cell Research2009，19，12： | 6 |
| 6 | Mixed lineage kinase domain-like protein induces RGC-5 necroptosis following elevated hydrostatic pressure显示文摘 | Lvshuang Liao Lei Shang Na Li Shuchao Wang Mi Wang Yanxia Huang Dan Chen Jufang Huang Kun Xiong | 2017 | Acta Biochimica et Biophysica Sinica2017，49，10： | 5 |
| 7 | Synthesis of 3,5-Dichloro-4-(1,1,2,2-tetrafluoroethoxy)phenyl Containing 1,2,3-Thiadiazole Derivatives via Ugi Reaction and Their Biological Activities显示文摘包含 4-methyl-1,2,3-thiadiazole 衍生物的一系列新奇 3,5-dichloro-4-(1,1,2,2-tetrafluoroethoxy ) 苯基经由 Ugi 反应被设计并且综合。他们的结构被红外, 1H NMR, 13C NMR 和高分辨率的集体光谱学证实。初步的生物鉴定结果显示一些标题混合物在 50 g/mL 举办了好杀真菌剂活动;大多数混合物在 500 g/mL 和 100 g/mL 对烟草马赛克病毒介绍了直接抑制活动,好 inactivation 和药品活动的某个度;一些混合物对 Plutella xylostella L 显示出好 larvicidal 活动。在对在 2 g/mL 的一种蚊虫 pipiens pallens 的 200 g/mL 和优秀 larvicidal 活动。 | Wang Shouxin Wang Huan Fan Zhijin Fu Yifeng Mi Na Zhang Jufang Zhang Zhengcai Belskaya Nataliya P. Bakulev Vasiliy A. | 2011 | Chinese Journal of Chemistry2011，29，2： | 4 |
| 8 | Characterization of the positive SARP family regulator PieR for improving piericidin A1 production in Streptomyces piomogeues var.Hangzhouwanensis显示文摘Piericidin A1,a member ofɑ-pyridone antibiotic,exhibits various biological activities such as antimicrobial,antifungal,and antitumor properties and possesses potent respiration-inhibitory activity against insects due to its competitive binding capacity to mitochondrial complex I.The biosynthetic pathway of piericidin A1 has been reported in Streptomyces piomogeues var.Hangzhouwanensis,while the regulatory mechanism remains poorly understood.In this study,a Streptomyces antibiotic regulatory protein(SARP)family transcriptional regulator PieR was characterized.Genetic disruption and complementation manipulations revealed that PieR positively regulated the production of piericidin A1.Moreover,the overexpression of pieR contributed to the improvement of piericidin A1 productivity.The real-time quantitative PCR(RT-qPCR)was carried out and the data showed that pieR stimulated the transcription of all the biosynthesis-related genes for piericidin A1.In order to explore the regulatory mechanism,electrophoresis mobility shift assays(EMSA)and DNase I footprinting experiments have been conducted.A protected region covering 50 nucleotides within the upstream region of pieR was identified and two 5-nt direct repeat sequences(5′-CCGGA-3′)in the protected region were found.These findings,taken together,set stage for transcriptional control engineering in the view of optimizing piericidin A1 production and thus provide a viable potent route for the construction of strains with high productivity. | Yan Li Lingxin Kong Jufang Shen Qing Wang Qian Liu Weinan Yang Zixin Deng Delin You | 2019 | Synthetic and Systems Biotechnology2019，4，1： | 4 |
| 9 | Sex disparity of lung cancer risk in non-smokers:a multicenter population-based prospective study based on China National Lung Cancer Screening Program显示文摘Background: Non-smokers account for a large proportion of lung cancer patients, especially in Asia, but the attention paid to them is limited compared with smokers. In non-smokers, males display a risk for lung cancer incidence distinct from the females-even after excluding the influence of smoking;but the knowledge regarding the factors causing the difference is sparse. Based on a large multicenter prospective cancer screening cohort in China, we aimed to elucidate the interpretable sex differences caused by known factors and provide clues for primary and secondary prevention.Methods: Risk factors including demographic characteristics, lifestyle factors, family history of cancer, and baseline comorbidity were obtained from 796,283 Chinese non-smoking participants by the baseline risk assessment completed in 2013 to 2018. Cox regression analysis was performed to assess the sex difference in the risk of lung cancer, and the hazard ratios (HRs) that were adjusted for different known factors were calculated and compared to determine the proportion of excess risk and to explain the existing risk factors.Results: With a median follow-up of 4.80 years, 3351 subjects who were diagnosed with lung cancer were selected in the analysis. The lung cancer risk of males was significantly higher than that of females;the HRs in all male non-smokers were 1.29 (95% confidence interval [CI]: 1.20-1.38) after adjusting for the age and 1.38 (95% CI: 1.28-1.50) after adjusting for all factors, which suggested that known factors could not explain the sex difference in the risk of lung cancer in non-smokers. Known factors were 7% (|1.29-1.38|/1.29) more harmful in women than in men. For adenocarcinoma, women showed excess risk higher than men, contrary to squamous cell carcinoma;after adjusting for all factors, 47% ([1.30-1.16]/[1.30-1]) and 4% ([7.02-6.75]/[7.02-1])) of the excess risk was explainable in adenocarcinoma and squamous cell carcinoma. The main causes of gender differences in lung cancer risk were lifestyle factors, baseline comorbidity, and family history.Conclusions: Significant gender differences in the risk of lung cancer were discovered in China non-smokers. Existing risk factors did not explain the excess lung cancer risk of all non-smoking men, and the internal causes for the excess risk still need to be explored;most known risk factors were more harmful to non-smoking women;further exploring the causes of the sex difference would help to improve the prevention and screening programs and protect the non-smoking males from lung cancers. | Zheng Wu Fengwei Tan Zhuoyu Yang Fei Wang Wei Cao Chao Qin Xuesi Dong Yadi Zheng Zilin Luo Liang Zhao Yiwen Yu Yongjie Xu Jiansong Ren Jufang Shi Hongda Chen Jiang Li Wei Tang Sipeng Shen Ning Wu Wanqing Chen Ni Li Jie He | 2022 | Chinese Medical Journal2022，，11： | 3 |
| 10 | In-cell infection: a novel pathway for Epstein-Barr virus infection mediated by cell-in-cell structures显示文摘 | Chao Ni Yuhui Chen Musheng Zeng Rongjuan Pei Yong Du Linquan Tang Mengyi Wang Yazhuo Hu Hanyu Zhu Meifang He Xiawei Wei Shan Wang Xiangkai Ning Manna Wang Jufang Wang Li Ma Xinwen Chen Qiang Sun Hong Tang Ying Wang Xiaoning Wang | 2015 | Cell Research2015，25，7： | 3 |
| 11 | Beta-amyloid precursor protein cleavage enzyme-1 expression in adult rat retinal neurons in the early period after lead exposure显示文摘Previous studies have reported that non-human primates and rodents exposed to lead during brain development may become dependent on the deposition of pre-determined β-amyloid protein (Aβ),and exhibit upregulation of β-site amyloid precursor protein expression in old age.However,further evidence is required to elucidate the precise relationship and molecular mechanisms underlying the effects of early lead exposure on excessive Aβ production in adult mammals.The present study investigated the effects of lead exposure on expression of β-amyloid precursor protein cleavage enzyme-1 (BACE-1) in the rat retina and the production of Aβ in early development,using the retina as a window for studying Alzheimer's disease.Adult rats were intraocularly injected with different doses of lead acetate (10μmol/L,100μmol/L,1 mmol/L,10 mmol/L and 100 mmol/L).The results revealed that retinal lead concentration,BACE-1 and its cleavage products β-C-terminal fragment and retina Aβ1-40 were all significantly increased in almost all of the lead exposure groups 48 hours later in a dose-dependent manner.The only exception was the 10μmol/L group.The distribution of BACE-1 in the retina did not exhibit obvious changes,and no distinctive increase in the activation of retinal microglia was apparent.Similarly,retinal synaptophysin expression did not exhibit any clear changes.These data suggest that lead exposure can result in the upregulation of retinal neuron BACE-1 expression in the early period of development and further increase the overproduction of Aβ1-40 in the retina.Our results provided novel insight into the molecular mechanisms underlying environmentally-induced Alzheimer's disease. | Jufang Huang Kai Huang Lei Shang Hui Wang Xiaoxin Yan Kun Xiong | 2011 | Neural Regeneration Research2011，6，14： | 3 |
| 12 | LabVIEW-based auto-timing counts virtual instrument system with ORTEC 974 Counter/Timer显示文摘In order to achieve the auto-timing counts measurement of nuclear radiation using ORTEC 974 Counter/Timer, an auto-timing counts virtual instrument system based on the LabVIEW virtual instrument development platform and GPIB instrument control and transmission bus protocol is designed in this paper. By introducing software timing technique, the minimum time base of factory setting improves from 0.1 s to 0.03 s. The timing counts performance and longtime stability are also discussed in detail. The automatic data recording and saving facilitates data analysis and processing. Its real-time display and statistic function is very convenient for monitoring the nuclear radiation. | YAN Jie LIU Rong LI Cheng JIANG Li LU Xinxin ZHU Tonghua WANG Mei WEN Zhongwei LIN Jufang | 2009 | Nuclear Science and Techniques2009，20，5： | 2 |
| 13 | Generation of sp3111 transgenic RNAi mice via permanent integration of small hairpin RNAs in repopulating spermatogonial cells in vivo显示文摘spermatid 特定的基因, sp3111,是 four-transmembrane 基因家庭的一个新成员。然而,它的繁殖生物功能留下逃犯。这研究的目的是为进一步在它的繁殖功能上学习 sp3111 基因表示的抑制的效果建立一个 sp3111 击倒的雄的老鼠模型。pSUPER-sp3111-shRNA 的 Recombinant 向量,对老鼠 sp3111 mRNA 的不同区域包含抄录功能的小发卡 RNA 顺序,被构造并且识别。然后,线性化的 recombinant 向量被 electroporation 注入成熟老鼠睾丸和 transfected 到 spermatogonial 房间产生 sp3111 转基因的 RNAi 老鼠。这些 electroporated 男性老鼠与野类型的女性一起被交配在 electroporation 和他们的子孙以后的 30 天被荧光显微镜学和 PCR 两个都检验。在 vivo 的 sp3111 mRNA 的睾丸表达式上的 RNAi 的抑制效率是超过30%并且能稳定地被传给 F3 产生,这被发现,并且与野类型的雄的鼠标相比,吗 sp3111 RNAi 雄的鼠标的后代的吝啬的数字从 11 被减少???????????????????????栠吗?? | Weili Shi Tingyan Shi Zheyu Chen Jufang Lin Xiaofeng Jia Jian Wang Huijuan Shi | 2010 | Acta Biochimica et Biophysica Sinica2010，42，2： | 2 |
| 14 | Recombinant Clostridium difficile toxin B induces endoplasmic reticulum stress in mouse colonal carcinoma cells显示文摘Clostridium 顽固在人和动物是联系抗菌素的腹泻和 pseudomembranous 大肠炎的主要原因。它的致病力首先被连接到二外毒素(TcdA 和 TcdB ) 的分泌物。尽管在 TcdA 和 TcdB 的有毒的机制的大进步被获得了,关于 apoptotic 机制有许多冲突报告。更重要地, apoptotic endoplasmic 蜂窝胃(嗯) 应力在与能引起腹泻和大肠炎的细胞毒素客气的 Shiga toxinsanother 对待的房间被报导了。此处,我们检查了 TcdB 是否能导致嗯应力。结果证明 recombinant TcdB (rTcdB ) 激活展开的蛋白质反应的分子的标记,建议导致的那 rTcdB 嗯在 CT26 房间的应力。然而, rTcdB 没导致 C/EBP 相应蛋白质(砍) 的起来规定, apoptotic 的一个经典调停人嗯应力,而是它激活半胱氨酸丁氨二酸的先锋酸特定的朊酶 12 (caspase-12 ) , apoptotic 的一个争论调停人嗯应力。而且,活动缺乏的变异的 recombinant TcdB 导致了的 glucosyltransferase 嗯应力,不过,它有不细胞毒素或 CT26 房间上的 cytopathic 效果。总的来说,这些数据表明了那嗯 rTcdB 导致的应力是 glucosyltransferase 独立的,显示那嗯 rTcdB 导致的应力是 non-apoptotic。这个工作也为我们提供新卓见进砍蛋白质表示规定和砍表示的角色的分子的机制在嗯应力。 | Chunli Sun Haiying Wang Shuyi Chen Zhendong Li Shan Li Jufang Wang | 2014 | Acta Biochimica et Biophysica Sinica2014，46，11： | 2 |
| 15 | Salubrinal protects against Clostridium difficile toxin B-induced CT26 cell death显示文摘Clostridium 顽固(C。顽固) 被认为在动物和人是联系抗菌素的腹泻和 pseudomembranous 大肠炎的主要原因。C 的流行。自从 2000,顽固感染(CDI ) 一直在增加。C 的二外毒素。顽固,毒素 A (TcdA ) 和毒素 B (TcdB ) , CDI 的主要毒力因素,能在宿主 cytosol 导致 Rho GTPases 的 glucosylation,正在导致细胞词法变化,细胞 apoptosis,和细胞死亡。导致 TcdB 的房间死亡的机制被调查十年了,但是它仍然不完全被理解。TcdB 经由在 CT26 房间线(BALB/C 老鼠冒号肿瘤房间) 表明小径的 PERK-eIF2 导致 endoplasmic 蜂窝胃应力,这被报导了。在这研究,我们发现了那 salubrinal, eIF2 dephosphorylation 的一个选择禁止者,高效地保护 CT26 房间线免于导致 TcdB 的房间死亡并且试着探索在这保护的效果位于下面的机制。我们的结果证明那 salubrinal 保护 CT26 房间免受的伤害调停 TcdB 细胞毒素并且 cytopathic 效果,经由 caspase-9-dependent 小径, eIF2 发信号小径,和 autophagy 禁止暴露毒素的房间的 apoptosis 和死亡。这些调查结果将对 CDI 治疗的发展有用。 | Shuyi Chen Chunli Sun Huawei Gu Haiying Wang Shan Li Yi Ma Jufang Wang | 2017 | Acta Biochimica et Biophysica Sinica2017，49，3： | 2 |
| 16 | Spatiotemporal alterations of presynaptic elements in the retina after high intraocular pressure显示文摘A rat model of acute high intraocular pressure was established by injecting saline into the anterior chamber of the left eye.Synaptophysin expression was increased in the inner plexiform layer at 2 hours following injury,and was widely distributed in the outer plexiform layer at 3-7 days,and then decreased to the normal level at 14 days.This suggests that expression of this presynaptic functional protein experienced spatiotemporal alterations after elevation of intraocular pressure.There was no significant change in the fluorescence intensity and distribution pattern for synapse-associated protein 102 following elevated intraocular pressure.Synapse-associated protein 102 immunoreactivity was confined to the outer plexiform layer,while synaptophysin immunoreactivity spread into the outer plexiform layer and the outer nuclear layer at 3 and 7 days following injury.These alterations in presynaptic elements were not accompanied by changes in postsynaptic components. | Jufang Huang Lihong Zhou Hui Wang Jia Luo Kun Xiong Leping Zeng Dan Chen | 2012 | Neural Regeneration Research2012，7，16： | 2 |
| 17 | Regulatory effects of inhibiting the activation of glial cells on retinal synaptic plasticity显示文摘Various retinal injuries induced by ocular hypertension have been shown to induce plastic changes in retinal synapses,but the potential regulatory mechanism of synaptic plasticity after retinal injury was still unclear.A rat model of acute ocular hypertension was established by injecting saline intravitreally for an hour,and elevating the intraocular pressure to 14.63 kPa(110 mmHg).Western blot assay and immunofluorescence results showed that synaptophysin expression had a distinct spatiotemporal change that increased in the inner plexiform layer within 1 day and spread across the outer plexiform layer after 3 days.Glial fibrillary acidic protein expression in retinae was greatly increased after 3 days,and reached a peak at 7 days,which was also consistent with the peak time of synaptophysin expression in the outer plexiform layer following the increased intraocular pressure.Fluorocitrate,a glial metabolic inhibitor,was intravitreally injected to inhibit glial cell activation following high intraocular pressure.This significantly inhibited the enhanced glial fibrillary acidic protein expression induced by high intraocular pressure injury.Synaptophysin expression also decreased in the inner plexiform layer within a day and the widened distribution in the outer plexiform layer had disappeared by 3 days.The results suggested that retinal glial cell activation might play an important role in the process of retinal synaptic plasticity induced by acute high intraocular pressure through affecting the expression and distribution of synaptic functional proteins,such as synaptophysin. | Lihong Zhou Hui Wang Jia Luo Kun Xiong Leping Zeng Dan Chen Jufang Huang | 2014 | Neural Regeneration Research2014，9，4： | 2 |
| 18 | Modeling of protein refolding from inclusion bodies显示文摘在 Escherichia coli 的外国蛋白质的 Overexpression 经常导致包括身体(IB ) 的形成,它在 recombinant 蛋白质和他们的应用的准备成为主要瓶颈。在现在的学习,从 IB 的 36 蛋白质是用一个简单 refolding 方法的 refolded。这些蛋白质的 Refolding 收益被定义为在冲淡进 refolding 缓冲区前在样品在使中毒的蛋白质的数量跟随冲淡 refolding 的可溶的蛋白质的百分比。而且,一个数学模型被推出在蛋白质 refolding 评估生物化学的性质的角色。自从一个单位的增长在 refolding 收益导致了 14.83% 的减少,我们的结果显示在试验性的条件下面,蛋白质的等电位的点可能主要正在贡献蛋白质 refolding 的高功效。另外的重要调停人是蛋白质的部件第二等的结构和分子的重量(R2 = 0.98, P = 0.000, F-test ) 。有在蛋白质 refolding 的低效率的六蛋白质拥有了相对低的等电位的点。而且,从 IB 的六另外的蛋白质的 refolding 收益被 refolding 在一样的条件下面预言并且进一步验证了蛋白质。因此,这里开发的蛋白质 refolding 的模型能被用来通过一个简单方法从 IB 预言蛋白质的 refolding 收益。我们的学习将是暗示的根据他们的内在的性质从 IB 为蛋白质 refolding 优化方法。 | Ting Zhang Xiaojing Xu Liang Shen Yanye Feng Zhong Yang Yaling Shen Jufang Wang Weirong Jin Xiaoning Wang | 2009 | Acta Biochimica et Biophysica Sinica2009，41，12： | 2 |
| 19 | A quick iso- lation method for mutants with high lipid yield in oleagi- nous yeast 显示文摘 | WANG Jufang LI Renmin DONG Lu | 2009 | World J Microbiol Biotechnol2009，25，5： | 1 |
| 20 | Optimization of culture medium for yellow pigments production with Monascus anka mutant using response surface methodology显示文摘 | Bo zhou Jufang Wang Yuewu Pu | 2009 | European Food Research and Technology2009，228，6： | 1 |
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