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| 1 | Overexpression of Sirt6 ameliorates sleep deprivation induced-cognitive impairment by modulating glutamatergic neuron function显示文摘Sleep benefits the restoration of energy metabolism and thereby suppo rts neuronal plasticity and cognitive behaviors.Sirt6 is a NAD+-dependent protein deacetylase that has been recognized as an essential regulator of energy metabolism because it modulates various transcriptional regulators and metabolic enzymes.The aim of this study was to investigate the influence of Sirt6 on cerebral function after chronic sleep deprivation(CSD).We assigned C57BL/6J mice to control or two CSD groups and subjected them to AAV2/9-CMV-EGFP or AAV2/9-CMV-Sirt6-EGFP infection in the prelimbic cortex(PrL).We then assessed cerebral functional connectivity(FC) using resting-state functional MRI,neuron/astrocyte metabolism using a metabolic kinetics analysis;dendritic spine densities using sparse-labeling;and miniature excitato ry postsynaptic currents(mEPSCs) and action potential(AP) firing rates using whole-cell patchclamp recordings.In addition,we evaluated cognition via a comprehensive set of behavioral tests.Compared with controls,Sirt6 was significantly decreased(P<0.05) in the PrL after CSD,accompanied by cognitive deficits and decreased FC between the PrL and accumbens nucleus,piriform cortex,motor co rtex,somatosensory co rtex,olfactory tubercle,insular cortex,and cerebellum.Sirt6 ove rexpression reve rsed CSD-induced cognitive impairment and reduced FC.Our analysis of metabolic kinetics using [1-13C] glucose and [2-13C] acetate showed that CSD reduced neuronal Glu4and GABA2synthesis,which could be fully restored via forced Sirt6 expression.Furthermore,Sirt6 ove rexpression reversed CSD-induced decreases in AP firing rates as well as the frequency and amplitude of mEPSCs in PrL pyramidal neurons.These data indicate that Sirt6 can improve cognitive impairment after CSD by regulating the PrL-associated FC network,neuronal glucose metabolism,and glutamatergic neurotransmission.Thus,Sirt6 activation may have potential as a novel strategy for treating sleep disorder-related diseases. | Jinpiao Zhu Chang Chen Zhen Li Xiaodong Liu Jingang He Ziyue Zhao Mengying He Binbin Nie Zili Liu Yingying Chen Kuanpin Su Xiang Li Juxiang Chen Hongbing Xiang Fuqiang Xu Kangguang Lin Zongze Zhang Jie Wang | 2023 | Neural Regeneration Research2023，18，11： | 1 |
| 2 | Overexpression of SLC7A7 predicts poor progression-free and overall survival in patients with glioblastoma显示文摘 | Songhua Fan Delong Meng Tao Xu Yuanyuan Chen Jingkun Wang Xiaoying Li Hongyan Chen Daru Lu Juxiang Chen Qing Lan | 2013 | Medical Oncology2013，，1： | 1 |
| 3 | MicroRNA-21 targets LRRFIP1 and contributes to VM-26 resistance in glioblastoma multiforme显示文摘 | Yiming Li Weiqing Li Yongji Yang Yicheng Lu Cheng He Guohan Hu Huimin Liu Juxiang Chen Jin He Hongyu Yu | 2009 | Brain Research2009，，： | 1 |
| 4 | Overexpression of Golgi phosphoprotein-3 (GOLPH3) in glioblastoma multiforme is associated with worse prognosis显示文摘 | Jinxu Zhou Tao Xu Rong Qin Yong Yan Chao Chen Yuanyuan Chen Hongyu Yu Chunyan Xia Yicheng Lu Xuehua Ding Yuhai Wang Xuejian Cai Juxiang Chen | 2012 | Journal of Neuro-Oncology2012，，2： | 1 |
| 5 | Overexpression of Golgi phosphoprotein-3 (GOLPH3) in glioblastoma multiforme is associated with worse prognosis显示文摘 | Jinxu Zhou Tao Xu Rong Qin Yong Yan Chao Chen Yuanyuan Chen Hongyu Yu Chunyan Xia Yicheng Lu Xuehua Ding Yuhai Wang Xuejian Cai Juxiang Chen | 2012 | Journal of Neuro-Oncology2012，，2： | 1 |
| 6 | Cloning, expression and mapping of the full-length cDNA of human CCTβ subunit显示文摘Chaperonins assist the proper folding of target proteins without being a part of the substrates. The eukaryotic cytosolic chaperonin, CCT-Chaperonin Containing TCP-1 (tailless complex polypeptide-1), is mainly involved in the formation of cytoskeletal proteins and is essential for cell viability. Mammalian CCT is commonly a protein complex composed of 7-9 subunit species. We have isolated a novel full-length cDNA from human testis cDNA library. This cDNA of 1935 bp contains a 1605 bp open reading frame (ORF) encoding 535 amino acids (aa). The deduced protein of the cDNA is highly homologous to the CCTβ subunit of saccharomyces cerevisiae, schizosaccharomyces pombe, caenorhabditis elegans and mouse, etc. Especially high homology (97%) is found between the deduced protein and mouse CCTβhomology, the protein encoded by the new gene was proposed to be a human CCTβ subunit. Northern hybridization showed that human CCTβ gene is expressed as a transcript of about 2.0 kb in various tissues. Overexpression | Juxiang Jiang Wei Lin Honglai Zhang Zheng Chen Qiang Tu Ying Jiang Long Yu Shouyuan Zhao | 2000 | Chinese Science Bulletin2000，45，22： | 0 |
| 7 | Lentiviral-mediated siRNA against osteopotin in U251 glioma cell line显示文摘Background:It has been reported that the expression of SPP1, the mRNA for osteopontin (OPN), is increased in a number of transformed cell lines and some tumors. Increased serum and plasma OPN levels were associated with advanced-stage carcinomas of the lung, breast, colon and prostate. The extent of OPN expression may be correlated with the malignancy degree of gliomas, but the specific role of OPN in the malignancy of gliomas remains to be clarified. Methods:In this study, we used a lentiviral system, which could induce a long-lasting down-regulation of gene expression, to knock down the OPN gene in human glioma cell line U251. And then we observed the proliferation and apoptosis of glioma cells. Results:OPN mRNA and protein were successfully knocked down in U251 cells, and the expression of caspase-3 and caspase-9 were increased about by 2 folds in OPN down-regulated U251 cells. 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium (MTT) assay showed that OPN down-regulation did not affect the proliferation of U251 cells. Conclusion:Our findings indicate that OPN might play a role in apoptosis, but not in cell proliferation in U251 glioma cells. | Xu Tao Chen Juxiang Lu Yicheng Zhou Jinxu Yan Yong Hu Guohan Luo Chun Huang Yan | 2010 | Journal of Medical Colleges of PLA(China)2010，25，1： | 0 |
| 8 | FOXO1-miR-506 axis promotes chemosensitivity to temozolomide and suppresses invasiveness in glioblastoma through a feedback loop of FOXO1/miR-506/ETS1/FOXO1显示文摘To explore the role of forkhead box protein O1(FOXO1)in the progression of glioblastoma multiforme(GBM)and related drug resistance,we deciphered the roles of FOXO1 and miR-506 in proliferation,apoptosis,migration,invasion,autophagy,and temozolomide(TMZ)sensitivity in the U251 cell line using in vitro and in vivo experiments.Cell viability was tested by a cell counting kit-8(CCK8)kit;migration and invasion were checked by the scratching assay;apoptosis was evaluated by terminal deoxynucleotidyl transferase dUTP nick-end labeling(TUNEL)staining and flow cytometry.The construction of plasmids and dual-luciferase reporter experiment were carried out to find the interaction site between FOXO1 and miR-506.Immunohistochemistry was done to check the protein level in tumors after the in vivo experiment.We found that the FOXO1-miR-506 axis suppresses GBM cell invasion and migration and promotes GBM chemosensitivity to TMZ,which was mediated by autophagy.FOXO1 upregulates miR-506 by binding to its promoter to enhance transcriptional activation.MiR-506 could downregulate E26 transformation-specific 1(ETS1)expression by targeting its 3'-untranslated region(UTR).Interestingly,ETS1 promoted FOXO1 translocation from the nucleus to the cytosol and further suppressed the FOXO1-miR-506 axis in GBM cells.Consistently,both miR-506 inhibition and ETS1 overexpression could rescue FOXO1 overactivation-mediated TMZ chemosensitivity in mouse models.Our study demonstrated a negative feedback loop of FOXO1/miR-506/ETS1/FOXO1 in GBM in regulating invasiveness and chemosensitivity.Thus,the above axis might be a promising therapeutic target for GBM. | Chao CHEN Yu’e LIU Hongxiang WANG Xu ZHANG Yufeng SHI Juxiang CHEN | 2023 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2023，24，8： | 0 |
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