|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Nanozymes: created by learning from nature显示文摘Nanozymes,a type of nanomaterials with enzyme-like activity,have shown great potential to replace natural enzymes in many fields such as biochemical detection,environmental management and disease treatment.However,the catalytic efficiency and substrate specificity of nanozymes still need improvement.To further optimize the enzymatic properties of nanozymes,recent studies have introduced the structural characteristics of natural enzymes into the rational design of nanozymes,either by employing small molecules to mimic the cofactors of natural enzymes to boost nanozymes’catalytic potential,or by simulating the active center of natural enzymes to construct the nanostructure of nanozymes.This review introduces the commonly used bio-inspired strategies to create nanozymes,aiming at clarifying the current progress and bottlenecks.Advances and challenges focusing on the research of bio-inspired nanozymes are outlined to provide ideas for the de novo design of ideal nanozymes. | Ruofei Zhang Kelong Fan Xiyun Yan | 2020 | Science China(Life Sciences)2020，63，8： | 13 |
| 2 | Nanozymes Inspired by Natural Enzymes显示文摘CONSPECTUS:Nanozymes,nanomaterials with enzyme-like activities with high structural stability,adjustable catalytic activity,functional diversity,recyclability,and feasibility in large-scale preparation,have become a hot spot in the field of artificial enzymes in recent years and are expected to become potential surrogates and competitors for natural enzymes in practical applications.With the development of in-depth research and a wide range of application requirements,creating nanozymes with catalytic performance comparable to or even surpassing that of natural enzymes has been the key research topic in this field.Most of the nanozymes reported in the past were obtained based on random synthesis and screening,for which the catalytic efficiency is far inferior to that of natural enzymes.Natural enzymes that have evolved over hundreds of millions of years have developed a lot of high-efficiency catalysis know-how hidden in their structural features.To create highly active nanozymes,we assumed that there is a general structure−activity relationship between nanozymes and natural enzymes and proposed the nanozyme optimization strategy by grafting the catalytic principles of natural enzymes into the rational design of nanozymes.On the basis of this bioinspired strategy,a series of nanozymes that exhibit similar catalytic activities that are closer to or even beyond those of natural enzymes have been successfully synthesized.By now,rationally designed high-activity bioinspired nanozymes have become a hot topic in the current research on nanozymes.In this Account,we focus on recent representative research progress in the systemic design and construction of bioinspired nanozymes and are devoted to introducing strategic concepts in the bioinspired optimization of nanozymes.We show that the de novo design of nanozymes by simulating the amino acid microenvironment and using metal-free architecture and the coordination structure of metal active sites in natural enzymes is an effective strategy for significantly improving the catalytic performance of nanozymes.A future perspective of the challenges and countermeasures of bioinspired nanozymes is proposed on the basis of these achievements.We hope that the biologically inspired perception will arouse widespread interest in fundamental research and practical applications as well as provide inspiration for the rational design of nanozymes. | Ruofei Zhang Xiyun Yan Kelong Fan | 2021 | Accounts of Materials Research2021，2，7： | 8 |
| 3 | Nanozymes: an emerging field bridging nanotechnology and enzymology显示文摘Nanozyme,a class of nanomaterials with intrinsic enzymelike properties,is a new concept which has been included in the Encyclopedia of China and the textbook of enzyme engineering.Since the first evidence published in 2007(Gao et al.,2007),great progress has been achieved in the study of nanozyme from new concept,new material to its new application,and it becomes an emerging field bridging nanotechnology and biology(Gao and Yan,2016). | Xiangqin Meng Kelong Fan Xiyun Yan | 2019 | Science China(Life Sciences)2019，62，11： | 8 |
| 4 | Mussel-inspired nanozyme catalyzed conductive and self-setting hydrogel for adhesive and antibacterial bioelectronics显示文摘Adhesive hydrogels have broad applications ranging from tissue engineering to bioelectronics;however,fabricating adhesive hydrogels with multiple functions remains a challenge.In this study,a mussel-inspired tannic acid chelated-Ag(TA-Ag)nanozyme with peroxidase(POD)-like activity was designed by the in situ reduction of ultrasmall Ag nanoparticles(NPs)with TA.The ultrasmall TA-Ag nanozyme exhibited high catalytic activity to induce hydrogel self-setting without external aid.The nanozyme retained abundant phenolic hydroxyl groups and maintained the dynamic redox balance of phenol-quinone,providing the hydrogels with long-term and repeatable adhesiveness,similar to the adhesion of mussels.The phenolic hydroxyl groups also afforded uniform distribution of the nanozyme in the hydrogel network,thereby improving its mechanical properties and conductivity.Furthermore,the nanozyme endowed the hydrogel with antibacterial activity through synergistic effects of the reactive oxygen species generated via POD-like catalytic reactions and the intrinsic bactericidal activity of Ag.Owing to these advantages,the ultrasmall TA-Ag nanozyme-catalyzed hydrogel could be effectively used as an adhesive,antibacterial,and implantable bioelectrode to detect bio-signals,and as a wound dressing to accelerate tissue regeneration while preventing infection.Therefore,this study provides a promising approach for the fabrication of adhesive hydrogel bioelectronics with multiple functions via mussel-inspired nanozyme catalysis. | Zhanrong Jia Xuanhan Lv Yue Hou Kefeng Wang Fuzeng Ren Dingguo Xu Qun Wang Kelong Fan Chaoming Xie Xiong Lu | 2021 | Bioactive Materials2021，6，9： | 7 |
| 5 | Zuogui pills for myelinolysis in a rat model of experimental autoimmune encephalomyelitis显示文摘Zuogui pills have been shown to attenuate the inflammatory reaction in a rat model of experimental autoimmune encephalomyelitis(EAE).The present study attempted to investigate the pathology underlying the influence of Zuogui pills on myelinolysis in EAE rats.Hematoxylin-eosin and Luxol fast blue staining showed that the myelinolysis foci in the cerebrum,cerebellum,brain stem,and the spinal cord of EAE rats were significantly decreased,along with serum myelin basic protein content following treatment with Zuogui pills. | Yongping Fan Kelong Chen Kangning Li Jianping Zhou Yan Shao Hongyan Liu WenjingYang | 2011 | Neural Regeneration Research2011，6，9： | 2 |
| 6 | Nanozyme:A promising tool from clinical diagnosis and environmental monitoring to wastewater treatment显示文摘Natural enzymes,owing to their outstanding catalytic efficiency and substrate specificity,have been used in a variety of applications including clinical diagnosis,environmental monitoring and wastewater treatment.However,they face inevitable problems such as relatively high cost and lack of stability,dramatically hindering their practical applications in the industry.Recently,a class of nanomaterial that possesses intrinsic enzyme-like properties,nanozyme,has emerged exhibiting numerous advantages over its natural counterpart and has been used as a viable enzyme alternative.In the past decade there are many reviews on nanozyme.The previous discussions tend to view nanozyme as a type of nanomaterial rather than an enzyme.However,it is the enzyme-like activity of nanozymes that provides foundation for their application and nanozymes with the same enzymatic activity usually have some regularity in application.Herein,in this review,we attempt to classify nanozymes by their enzyme-like activity to explain the application principle and relevant cases of nanozymes in clinical diagnosis,environmental monitoring and wastewater treatment,expecting to promote deeper thinking of nanozymes as enzyme mimics and provide useful guidance for future research. | Chaoyi Hong Xiangqin Meng Jiuyang He Kelong Fan Xiyun Yan | 2022 | Particuology2022，，12： | 2 |
| 7 | Bioengineered magnetoferritin nanozymes for pathological identification of high-risk and ruptured atherosclerotic plaques in humans显示文摘Atherosclerotic plaque rupture results in thrombus formation and vessel occlusion, and is the leading cause of death worldwide. There is a pressi ng need to identify plaque vuln erability for the treatment of carotid and coronary artery diseases. Nano materials with en zyme-like properties have attracted significant interest by providing biological, diagnostic and prognostic information about the diseases. Here we showed that bioe ngin eered mag netoferritin nan oparticles (M-HFn NPs) functionally mimic peroxidase en zyme and can intrin sically recog nize plaque-infiltrated active macrophages, which drive atherosclerotic plaque progression and rupture and are significantly associated with the plaque vulnerability. The M-HFn nanozymes catalyze the oxidation of colorimetric substrates to give a color reaction that visualizes the recognized active macrophages for one-step pathological identification of plaque vulnerability. We examined 50 carotid endarterectomy specimens from patients with symptomatic carotid disease and demonstrated that the M-HFn nanozymes could distinguish active macrophage infiltration in ruptured and high-risk plaque tissues, and M-HFn staining displayed a significant correlation with plaque vulnerability (r= 0.89, P< 0.0001). | Tao Wang Jiuyang He Demin Duan Bing Jiang Peixia Wang Kelong Fan Minmin Liang Xiyun Yan | 2019 | Nano Research2019，12，4： | 1 |
| 8 | CD146 is a coreceptor for VEGFR-2 in tumor angiogenesis显示文摘 | Tianxia Jiang Jie Zhuang Hongxia Duan Yongting Luo Qiqun Zeng Kelong Fan Huiwen Yan Di Lu Zhongde Ye Junfeng Hao Jing Feng Dongling Yang Xiyun Yan | 2012 | Blood2012，，11： | 1 |
| 9 | Preparation of silver-modified La 0.6 Ca 0.4 CoO 3 binary electrocatalyst for bi-functional air electrodes in alkaline medium显示文摘 | Shuxin Zhuang Kelong Huang Chenghuan Huang Hongxia Huang Suqin Liu Min Fan | 2010 | Journal of Power Sources2010，，8： | 1 |
| 10 | Bioinspired metal-organic framework nanozyme reinforced with thermosensitive hydrogel for regulating inflammatory responses in Parkinson’s disease显示文摘Parkinson’s disease(PD)is a prevalent neurodegenerative disorder accompanied by movement disorders and neuroinflammatory injury.Anti-inflammatory intervention to regulate oxidative stress in the brain is beneficial for managing PD.However,traditional natural antioxidants have failed to meet the clinical treatment demands due to insufficient activity and sustainability.Herein,Cu-doping zeolite imidazolate framework-8(ZIF-8)nanozyme is designed to simulate Cu/Zn superoxide dismutase(SOD)by biomimetic mineralization.The nanozyme composite is then integrated into thermosensitive hydrogel(poly(lactic-co-glycolic acid)-poly(ethylene glycol)-poly(lactic-co-glycolic acid)(PLGA-PEG-PLGA))to form an effective antioxidant system(Cu-ZIF@Hydrogel).The thermosensitive hydrogel incorporating nanozymes demonstrate distinct viscoelastic properties aimed at enhancing local nanozyme adhesion,prolonging nanozyme retention time,and modulating antioxidant activity,thus significantly improving the bioavailability of nanozymes.At the cellular and animal levels of PD,we find that Cu-ZIF@Hydrogel bypass the blood-brain barrier and efficiently accumulate in the nerve cells.Moreover,the Cu-ZIF@Hydrogel significantly alleviate the PD’s behavioral and pathological symptoms by reducing the neuroinflammatory levels in the lesion site.Therefore,the hydrogel-incorporating nanozyme system holds great potential as a simple and reliable avenue for managing PD. | Xiaowan Fan Tao Zhang Xin Ding Yushuo Gu Qing Li Wei Jiang Kelong Fan | 2024 | Nano Research2024，17，2： | 1 |
| 11 | Black phosphorus quantum dots as multifunctional nanozymes for tumor photothermal/catalytic synergistic therapy显示文摘Nanozymes are nanomaterials with enzyme-like properties that have attracted significant interest owing to their high stability,easy preparation,and tunable catalytic properties,especially in the field of cancer therapy.However,the unfavorable catalytic effects of nanozymes in the acidic tumor microenvironment have limited their applications.Herein,we developed a biomimetic erythrocyte membrane-camouflaged ultrasmall black phosphorus quantum dots(BPQDs)nanozymes that simultaneously exhibited an exceptional near-infrared(NIR)photothermal property and dramatically photothermal-enhanced glucose oxidase(GOx)-like activity in the acidic tumor microenvironment.We demonstrated the engineered BPQDs gave a photothermal conversion efficiency of 28.9%that could rapidly heat the tumor up to 50℃ while effectively localized into tumors via homing peptide iRGD leading after intravenously injection.Meanwhile,the significantly enhanced GOx-like activity of BPQDs under NIR irradiation was capable of catalytical generating massive toxic reactive oxygen species via using cellular glucose.By combining the intrinsic photothermal property and the unique photothermal-enhanced GOx-like catalytic activity,the developed BPQDs were demonstrated to be an effective therapeutic strategy for inhibiting tumor growth in vivo.We believe that this work will provide a novel perspective for the development of nanozymes in tumor catalytic therapy. | Hui Ding Daji Wang Haibing Huang Xiaozhu Chen Jie Wang Jinjie Sun Jianlin Zhang Lu Lu Beiping Miao Yanjuan Cai Kelong Fan Yongtian Lu Hongsong Dong Xiyun Yan Guohui Nie Minmin Liang | 2022 | Nano Research2022，15，2： | 1 |
| 12 | Ferritin nanocages for early theranostics of tumors via inflammation-enhanced active targeting显示文摘Engineered nanocarriers have been widely developed for tumor theranostics.However,the delivery of imaging probes or therapeutic drugs to the tumor pre-formation site for early and accurate detection and therapy remains a major challenge.Here,by using tailor-functionalized human H-ferritin(HFn),we developed a triple-modality nanoprobe IRdye800-M-HFn and achieved the early imaging of tumor cells before the formation of solid tumor tissues.Then,we developed an HFn-doxorubicin(Dox)drug delivery system by loading Dox into the HFn protein cage and achieved early-stage tumor therapy.The intravenous injection of HFn nanoprobes enabled the imaging of tumor cells as early as two days after tumor implantation,and the triple-modality imaging techniques,namely,near-infrared fluorescence molecular imaging(NIR-FMI),magnetic resonance imaging(MRI),and photoacoustic imaging(PAI),ensured the accuracy of detection.Further exploration indicated that HFn could specifically penetrate into pre-solid tumor sites by tumor-associated inflammation-mediated blood vessel leakage,followed by effective accumulation in tumor cells by the specific targeting property of HFn to transferrin receptor 1.Thus,the HFn-Dox drug delivery system delivered Dox into the tumor pre-formation site and effectively killed tumor cells at early stage.IRDye800-M-HFn nanoprobes and HFn-Dox provide promising strategies for early-stage tumor diagnosis and constructive implications for early-stage tumor treatment. | Bing Jiang Xiaohua Jia Tianjiao Ji Meng Zhou Jiuyang He Kun Wang Jie Tian Xiyun Yan Kelong Fan | 2022 | Science China(Life Sciences)2022，65，2： | 0 |
| 13 | Erratum to:Black phosphorus quantum dots as multifunctional nanozymes for tumor photothermal/catalytic synergistic therapy显示文摘Erratum to Nano Research 2022,15(2):1554–1563 https://doi.org/10.1007/s12274-021-3701-8 Figure 3(d)in the original paper contained duplicated micrographs(BPQDs+NIR)for different xenografts(B16 vs.CNE-2).This error did not affect any of the conclusions from the published paper. | Hui Ding Daji Wang Haibing Huang Xiaozhu Chen Jie Wang Jinjie Sun Jianlin Zhang Lu Lu Beiping Miao Yanjuan Cai Kelong Fan Yongtian Lu Hongsong Dong Xiyun Yan Guohui Nie Minmin Liang | 2023 | Nano Research2023，16，1： | 0 |
| 14 | The prototypes of nanozyme-based nanorobots显示文摘Artificial nanorobot is a type of robots designed for executing complex tasks at nanoscale.The nanorobot system is typically consisted of four systems,including logic control,driving,sensing and functioning.Considering the subtle structure and complex functionality of nanorobot,the manufacture of nanorobots requires designable,controllable and multi-functional nanomaterials.Here,we propose that nanozyme is a promising candidate for fabricating nanorobots due to its unique properties,including flexible designs,controllable enzyme-like activities,and nano-sized physicochemical characters.Nanozymes may participate in one system or even combine several systems of nanorobots.In this review,we summarize the advances on nanozyme-based systems for fabricating nanorobots,and prospect the future directions of nanozyme for constructing nanorobots.We hope that the unique properties of nanozymes will provide novel ideas for designing and fabricating nanorobotics. | Jiaying Xie Yiliang Jin Kelong Fan Xiyun Yan | 2020 | Biophysics Reports2020，6，6： | 0 |
| 15 | Corrigendum to“Mussel-inspired nanozyme catalyzed conductive and self-setting hydrogel for adhesive and antibacterial bioelectronics”[Bioact.Mater.6(2021)2676-2687/452]显示文摘The authors regret | Zhanrong Jia Xuanhan Lv Yue Hou Kefeng Wang Fuzeng Ren Dingguo Xu Qun Wang Kelong Fan Chaoming Xie Xiong Lu | 2023 | Bioactive Materials2023，，5： | 0 |
| 16 | A Metal-free Nanozyme-activated Prodrug Strategy for Targeted Tumor Catalytic Therapy显示文摘Enzyme prodrug therapy,which was introduced in 1985,is an attractive strategy for targeted cancer therapy with low systemic toxicity.The prodrug itself is low-toxic or non-toxic,after it was ingested,the enzyme can selectively activate the prodrug at the site of the lesion,thus enhancing the pharmacological effect and reducing the side effects. | FAN Kelong | 2020 | Bulletin of the Chinese Academy of Sciences2020，34，4： | 0 |
| 17 | Questions about horse the blood brain barrier receptor 1 spleen ferritin crossing via mouse transferrin显示文摘 | Kelong Fan Meng Zhou Xiyun Yan | 2017 | Protein & Cell2017，8，11： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员