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| 1 | The FTO/miR-181b-3p/ARL5B signaling pathway regulates cell migration and invasion in breast cancer显示文摘Background:N6-methyladenosine(m6A)RNA modification has been demonstrated to be a significant regulatory process in the progression of various tumors,including breast cancer.Fat mass and obesity-associated(FTO)enzyme,initially known as the obesity-related protein,is the first identified m6A demethylase.However,the relationship between FTO and breast cancer remains controversial.In this study,we aimed to elucidate the role and clinical significance of FTO in breast cancer and to explore the underlying mechanism.Methods:We first investigated the expression of FTO in breast cancer cell lines and tissues by quantitative reverse transcription-PCR(qRT-PCR),Western blotting,and immunohistochemistry.Wound healing assay and Transwell assay were performed to determine the migration and invasion abilities of SKBR3 and MDAMB453 cells with either knockdown or overexpression of FTO.RNA sequencing(RNA-seq)was conducted to decipher the downstream targets of FTO.qRT-PCR,luciferase reporter assay,and Western blotting were employed to confirm the existence of the FTO/miR-181b-3p/ARL5B axis.The biological function of ADP ribosylation factor like GTPase 5B(ARL5B)in breast cancer cells was evaluated by wound healing assay and Transwell invasion assay.Results:High FTO expression was observed in human epidermal growth factor receptor 2(HER2)-positive breast cancer,predicting advanced progression(tumor size[P<0.001],nuclear grade[P=0.001],peritumoral lymphovascular invasion[P<0.001),lymph node metastasis[P=0.002],and TNM stage[P=0.001])and poor prognosis.Moreover,FTO promoted cell invasion and migration in vitro.Mechanistically,RNA-seq and further confirmation studies suggested that FTO up-regulated ARL5B by inhibiting miR-181b-3p.We further verified that ARL5B also displayed carcinogenic activity in breast cancer cells.Conclusion:Our work demonstrated the carcinogenic activity of FTO in promoting the invasion and migration of breast cancer cells via the FTO/miR-181b-3p/ARL5B signaling pathway. | Yuanyuan Xu Shuang Ye Nan Zhang Shuhui Zheng Huatao Liu Kewen Zhou Ling Wang Yue Cao Peng Sun Tinghuai Wang | 2020 | Cancer Communications2020，40，10： | 24 |
| 2 | Association between AKT1 gene polymorphisms and depressive symptoms in the Chinese Han population with major depressive disorder显示文摘For this study, 461 Chinese Han patients with depressive disorder were recruited. The AKT1 genotype and allele distribution were determined by PCR amplification and direct sequencing. UNPHASED software was used to analyze associations between the 17-item Hamilton Depression Rating Scale, total score, four factors and the AKT1 rs2494746 and rs3001371 polymorphisms. The results indicate that there is a significant association between suicidal ideation and anxiety symptoms in depressed patients and the rs2494746 polymorphism. The other AKT1 polymorphism, rs3001371, was significantly associated with work and activities. Patients with the rs3001371-A allele had a significantly more severe illness compared to patients with the rs3001371-G allele. Thus, AKT1 polymorphisms appear to be associated with depression severity, anxiety symptoms, work and activities, and suicide attempts in patients with depressive disorder. | Chunxia Yang Ning Sun Yan Ren Yan Sun Yong Xu Aiping Li Kewen Wu Kerang Zhang | 2012 | Neural Regeneration Research2012，7，3： | 8 |
| 3 | Equilibrium of liquid-liquid extraction of 2-phenylbutyric acid enantiomers: Experiment and model显示文摘The utilization of liquid–liquid extraction for the separation of 2-phenylbutyric acid(2-PBA) enantiomers was proposed. Factors affecting the extract process were investigated, including organic solvents, β-cyclodextrin derivatives, cyclodextrin concentration, p H and temperature. A model was proposed to describe the separation process based on the homogeneous phase reaction mechanism. Important parameters of this model were determined experimentally. The physical distribution coefficients for molecular and ionic 2-PBA were0.129 and 7.455, respectively. The equilibrium constants of the complexation reactions were 89.36 and36.78 L·mol^(-1) for(+)-and(-)-2-PBA, respectively. The model was verified by experiments and proved to be an excellent means to optimize the separation system. Through modeling prediction and experiment, the best conditions(e.g., pH value of 3.00, extractant concentration of 0.1 mol·L^(-1), temperature of 5.0 °C) were acquired. Under this condition, the maximum enantioselectivity(2.096) was obtained. | Weifeng Xu Guilin Dai Kewen Tang Panliang Zhang Biquan Xiong Yu Liu | 2018 | Chinese Journal of Chemical Engineering2018，26，4： | 2 |
| 4 | Estrogen improved metabolic syndrome through down-regulation of VEGF and HIF-1α to inhibit hypoxia of periaortic and intra-abdominal fat in ovariectomized female rats显示文摘 | JinWen Xu QiuLing Xiang GuiPing Lin XiaoDong Fu KeWen Zhou Ping Jiang ShuHui Zheng TingHuai Wang | 2012 | Molecular Biology Reports2012，，8： | 1 |
| 5 | Musks and organochlorine pesticides in breast milk from Shanghai, China: Levels, temporal trends and exposure assessment显示文摘 | Jing Zhou Xiangying Zeng Kewen Zheng Xuejing Zhu Li Ma Qing Xu Xiaolan Zhang Yingxin Yu Guoying Sheng Jiamo Fu | 2012 | Ecotoxicology and Environmental Safety2012，，： | 1 |
| 6 | Ecological wetland paradigm drives water source improvement in the stream network of Yangtze River Delta显示文摘Jiaxing created a precedent using bypass riparian marshes to purify micro-polluted water sources in China.Pond-wetland complex with constructed root channel technology becomes a paradigm which can be analogized as'human-body wetland model'based on bionics or biomimetics.Heterogeneous plant-bed/ditch system with highly active land/water ecotone interfaces,especially meandering boundaries,breeds many biochemical reactions'living areas'.Optimization of hydraulic regulation promotes redox environment alternations and wetland treatment efficiency.Here we reported a series of upgrades and performances in Guanjinggang wetland after the Shijiuyang prototype.Morphological reform of plant-bed/ditch system played a vital role.Spatially root channel zone was main force of wetland purification,and temporally the treatment effect was higher in low-temperature seasons indicating non-temperature dependent mechanisms worked.Water pollution comprehensive index improved steadily from IV to III,and comprehensive pollution load was reduced by ca.40%–60%.Comprehensive evaluation function value further showed the gradients purification effect of the upgraded wetland.Ecological wetlands ameliorated source water quality,and reduced drinking water treatment reagents,thereby bringing about economic benefits.Through wetlands operation,people can see how the micro-polluted surface water becomes clear and clean,so promoting a significant social benefit.As a viable component of urban green space,wetlands could beautify regional eco-environment,freshen the air,increase urban ecological taste,and enhance the eco-environmental protection publicity.Thus,the multifunctional service values and indirect benefits are substantial.Jiaxing ecological wetlands provide a typical paradigm for water pollution remediation in developing countries and plays a leading role in technology engineering radiation effect. | Weidong Wang Ting Yang Weibing Guan Weixi Peng Ping Wu Bin Zhong Chundong Zhou Qinghua Chen Rongbin Zhang Kewen Xu Chengqing Yin | 2021 | Journal of Environmental Sciences2021，33，12： | 1 |
| 7 | Optimal Energy-Efficient Power Allocation for OFDM-based Cognitive Radio Networks显示文摘 | WANG Van XU Wenjun YANG Kewen | 2012 | IEEE Communications Letters2012，16，9： | 1 |
| 8 | Comparative metabolomics analysis in the clean label ingredient of NFC spine grape juice processed by mild heating vs high pressure processing显示文摘Not from concentrate(NFC)fruit juice is the crucial clean label ingredient for new-style tea-making due to its pleasant color and fresh aroma.Here,we compared the effects of mild heating(MH)and high pressure processing(HPP)on physicochemical characters and phytochemicals in NFC spine grape juice based on metabolomics analysis.Similar compound profiles were observed between HPP-treated and fresh juices.The richer phytochemical compounds comprised malvidin-3-O-glucoside,malvidin-3,5-di-O-glucoside,quercetin-3-O-rhamnoside,quercetin-3-Oglucuronide,catechin,caffeic acid,ferulic acid,procyanidin B1,procyanidin B2 were obtained after MH treatment.Nine marker phenolics and two marker tripeptides(i.e.,Glu-Val-Phe and Leu-Leu-Tyr)were identified to differentiate MH from HPP treatment,of which higher contents occurred in the MH group.Storage time experiments showed that the Glu-Val-Phe could serve as potential markers for monitoring storage of spine grape juice.These results provide new insights into the effects of processing on individual phytochemical changes and the guide for commercial application of production of spine grape NFC juice. | Shini Yang Lu Mi Kewen Wang Xue Wang Jihong Wu Meijun Wang Zhenzhen Xu | 2023 | Food Innovation and Advances2023，2，2： | 0 |
| 9 | Study on extraction kinetics ofα-cyclopentylmandelic acid enantiomers with hydroxyethyl-β-cyclodextrin as chiral selector显示文摘In this work,the kinetic study on reactive extraction ofα-cyclopentylmandelic acid(α-CPMA)enantiomers was performed in a Lewis cell using hydroxyethyl-β-cyclodextrin(HE-β-CD)as chiral selector.The enantioselective complexation equilibrium between HE-β-CD andα-CPMA enantiomers was studied by phase solubility method.The important process parameters affecting the initial extraction rate were separately studied and the reaction rate equations were deduced.The optimal conditions for kinetic study were as follows:stirring speed of 75 r·min^(-1),interfacial area of 12.56 cm^2,pH of 2.5,initial HE-β-CD concentration of 0.05 mol·L^(-1),initialα-CPMA concentration of 5 mmol·L^(-1),and temperature of 278 K.The reaction has been found to be first order inα-CPMA and second order in HE-β-CD with the forward rate constants of 2.056×10^(-3)m^6·mol^(-2)·s^(-1)and 1.459×10^(-3)m^6·mol^(-2)·s^(-1)for(S)-α-CPMA and(R)-α-CPMA,respectively.The complexation equilibrium constants were evaluated as 61 L·mol^(-1) and 117 L·mol^(-1)for(S)-α-CPMA and(R)-α-CPMA,and the intrinsic enantioselectivity is estimated as 1.92. | Panliang Zhang Pan Jiang Weifeng Xu Yu Liu Biquan Xiong Yunren Qiu Kewen Tang | 2018 | Chinese Journal of Chemical Engineering2018，26，1： | 0 |
| 10 | Vertical graphene-coated Cu wire for enhanced tolerance to high current density in power transmission显示文摘Cu wires(CuWs)are widely used as electric transmission lines.However,their limited thermal and chemical stabilities become challenges under the high-power and harsh environment.Graphene is regarded as an ideal protective barrier for CuW benefiting from its impermeability to all atoms and molecules.Particularly,the excellent hydrophobicity of vertical graphene(VG)will strengthen its protective capability as a corrosion and oxidation barrier.Herein,VG is directly synthesized on CuW by plasmaenhanced chemical vapor deposition method.The hydrophobic VG coating with a high water contact angle can effectively exclude the corrosive liquid and moisture from CuW surface and prevent their further penetration.Consequently,the electrochemical corrosion rate of VG-CuW is reduced by~13,8,and 2 times,compared with bare CuW,VG-CuW with hydrophilic treatment,and CuW coated with thick horizontal graphene layers,respectively.Negligible oxidation occurs on VGCuW after the long-time exposure to humid air at~200℃ along with the largely enhanced tolerance under high-current operating condition.This study reveals the impressive potentials of hydrophobic VG as a robust corrosion and oxidation barrier for metal wires used in high-power cables and electronic devices in harsh environment. | Kun Wang Shuting Cheng Qingmei Hu Feng Yu Yi Cheng Kewen Huang Hao Yuan Jun Jiang Wenjuan Li Junliang Li Shichen Xu Jianbo Yin Yue Qi Zhongfan Liu | 2022 | Nano Research2022，15，11： | 0 |
| 11 | Development of a New in-situ Interface Shear Box Test Apparatus and Its Applications显示文摘INTRODUCTION Interfaces are often seen in various geotechnical materials,such as soil-bedrock interfaces(Yang et al.,2020;Gabet et al.,2006),soil-structure interfaces(Ilori et al.,2017;di Donna et al.,2016),and interfaces between soils with different properties(Zhu et al.,2022). | Kewen Yu Ling Xu Jianbing Peng Lu Zuo Guangxi Guan | 2023 | Journal of Earth Science2023，34，3： | 0 |
| 12 | Enantioselective esterification of(R,S)-2-(4-methylphenyl)propionic acid via Novozym 435:Optimization and application显示文摘This paper reports on the resolution of(R,S)-2-(4-methylphenyl)propionic acid(MPPA)enantiomers by enzymatic esterification in organic solvent.Novozym 435(CALB)has the best catalytic performance compared with other lipases.Of the alcohols screened,n-hexanol is the best acyl acceptor and gives the highest enzyme activity and enantioselectivity in n-hexane.Response surface methodology(RSM)was used to evaluate the influence of the factors,such as temperature,enzyme amount,substrate concentration and reaction time on the substrate conversion(c)and enantiomeric excess(ee).The correlation coefficient R2 for enantiomeric excess and the conversion are 0.9827 and 0.9910,respectively,indicating that can accurately predict the experimental results.By simulation and optimization,the optimal conditions were obtained,involving 600 mmol·L^-1 MPPA concentration(0.60 mmol),850 mmol·L^-1 hexanol concentration(0.85 mmol),58 mg enzyme amount,75℃temperature and 4.5 h reaction time,respectively.Under the optimized conditions,the experimental values of conversion and enantiomeric excess were 89.34%and 97.84%,respectively,which are in good agreement with the model predictions. | Xin Yuan LujunWang Panliang Zhang Weifeng Xu Kewen Tang | 2020 | Chinese Journal of Chemical Engineering2020，28，7： | 0 |
| 13 | The Development of Mongolian as a Minority Language in Digital Spaces显示文摘1 What is minority language?Chaklader(1981)argues for adopting a definition of minority languages at the state level.A‘minority language’,in the most straightforward sense,is simply one language spoken by less than 50 percent of a population within a specific geographic region which is different from the language of the majority community and the language of the state[1].The crucial point is the proportion of speaker population in the given region or country.In other words,a minority language might be only a minority language in this specific region,but a majority language in other region(Grenoble,2014)[2].For example,Spanish is the majority language in a group of south American countries,however,it is a minority language in the USA in general.Mongolian,is also an example of this kind of minority language. | Kewen Xu | 2020 | Journal of Contemporary Educational Research2020，4，2： | 0 |
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