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| 1 | Correction of a genetic disease by CRISPR-Cas9-mediated gene editing in mouse spermatogonial stem cells显示文摘Spermatogonial 干细胞(SSC ) 能在移植以后生产众多的男配偶子进接受者睾丸,介绍为基因治疗和修改基因的动物的连续生产的一条珍贵途径。然而, SSC 的成功的基因操作被限制了,部分由于复杂性和当前可得到的基因编辑技术的低效率。这里,我们证明有效基因修正能用 CRISPR-Cas9 系统被介绍进 SSC。我们使用了 CRISPR-Cas9 系统变异在 SCC 的 EGFP transgene 或内长的 Crygc 基因。变异的 SSC 在移植以后经历了精子发生进不肥沃的老鼠睾丸的生精的小管。圆 spermatids 被产生并且在进成熟卵母细胞的注射以后,支持了显示相应变异的显型的异质接合的后代的生产。而且,在 Crygc 的一个引起疾病的变化(在 SSC 先存在的 Crygc −/−) 能乐意地到加入的 CRISPR-Cas9-induced nonhomologous 结束(NHEJ ) 或指导相同的修理(HDR ) 被修理,导致没有是的离开目标修正的证据带改正的基因的 SSC 线由整个染色体的定序出现。用从这些线产生的圆 spermatids 的授精在 100% 的效率与改正的显型产生了后代。我们的结果表明有效基因由 CRISPR-Cas9 系统在老鼠 SSC 编辑,并且提供在 SSC 经由基因修正治好基因疾病的原则的证明。 | Yuxuan Wu Hai Zhou Xiaoying Fan Ying Zhang Man Zhang Yinghua Wang Zhenfei Xie Meizhu Bai Qi Yin Dan Liang Wei Tang Jiaoyang Liao Chikai Zhou Wujuan Liu Ping Zhu Hongshan Guo Hong Pan Chunlian Wu Huijuan Shi Ligang Wu Fuchou Tang Jinsong Li | 2015 | Cell Research2015，25，1： | 37 |
| 2 | Effect of hepatocyte apoptosis induced by TNF-α on acute severe hepatitis in mouse models显示文摘AIM To study the effect of hepatocyteapoptosis and necrosis induced by TNF-α on thepathogenesis of acute severe hepatitis(ASH).METHODS The model of ASH was prepared inD-galactosamine(GAIN)sensitized BALB/c miceby injection of either endotoxin(ET)or tumornecrosis factor-α(TNF-α).Morphologicalchanges of apoptotic hepatocytes were studiedby both light and electron microscope and in siteend labeling method(ISEL).Molecular biologicalchanges of DNA ladder were observed byelectrophoresis of extract from liver tissues.Biochemical changes were measured by alanineaminotransferase(ALT),asparticaminotransferase(AST)and TNF-α.The relationbetween apoptosis and necrosis was evaluatedsimultaneously.RESULTS The sequence of hepatocyteapoptosis,necrosis,and final death from ASHwas observed both in GAIN/ET and GAIN/TNF-agroup.Apoptosis was prominent at 3.5 h and 5 hafter injection of inducer,while necrosis becamedominant at 9 h after challenge.The appearanceof apoptosis was earlier in GAIN/TNF-α groupthan that in GAIN/ ET group.Pretreatment ofmice with antiTNF IgG1 may completely preventthe liver injury induced by GalN/ET.CONCLUSION TNF-α can cause liver damageby inducing hepatic apoptosis and necrosis inmice with endotoxemia. | Guo Qing Zang Xia Qiu Zhou Hong Yu Qing Xie Guo Ming Zhao Bin Wang Qing Guo Yue Qin Xiang Dan Liao Department of Infectious Diseases,Rujin Hospital,Shanghai Second Medical University,Shanghai 200025,China | 2000 | World Journal of Gastroenterology2000，6，5： | 30 |
| 3 | Single-cell RNA-seq highlights intra-tumoral heterogeneity and malignant progression in pancreatic ductal adenocarcinoma显示文摘Pancreatic ductal adenocarcinoma (PDAC) is the most common type of pancreatic cancer featured with high intra-tumoral heterogeneity and poor prognosis. To comprehensively delineate the PDAC intra-tumoral heterogeneity and the underlying mechanism for PDAC progression, we employed single-cell RNA-seq (scRNA-seq) to acquire the transcriptomic atlas of 57,530 individual pancreatic cells from primary PDAC tumors and control pancreases, and identified diverse malignant and stromal cell types, including two ductal subtypes with abnormal and malignant gene expression profiles respectively, in PDAC. We found that the heterogenous malignant subtype was composed of several subpopulations with differential proliferative and migratory potentials. Cell trajectory analysis revealed that components of multiple tumor-related pathways and transcription factors (TFs) were differentially expressed along PDAC progression. Furthermore, we found a subset of ductal cells with unique proliferative features were associated with an inactivation state in tumor-infiltrating T cells, providing novel markers for the prediction of antitumor immune response. Together, our findings provide a valuable resource for deciphering the intra-tumoral heterogeneity in PDAC and uncover a connection between tumor intrinsic transcriptional state and T cell activation, suggesting potential biomarkers for anticancer treatment such as targeted therapy and immunotherapy. | Junya Peng Bao-Fa Sun Chuan-Yuan Chen Jia-Yi Zhou Yu-Sheng Chen Hao Chen Lulu Liu Dan Huang Jialin Jiang Guan-Shen Cui Ying Yang Wenze Wang Dan Guo Menghua Dai Junchao Guo Taiping Zhang Quan Liao Yi Liu Yong-Liang Zhao Da-Li Han Yupei Zhao Yun-Gui Yang Wenming Wu | 2019 | Cell Research2019，29，9： | 24 |
| 4 | Phase 1 clinical trial demonstrated that MUC1 positive metastatic seminal vesicle cancer can be effectively eradicated by modified Anti-MUC1 chimeric antigen receptor transduced T cells显示文摘Recent progress in chimeric antigen receptor-modified T-cell(CAR-T cell) technology in cancer therapy is extremely promising, especially in the treatment of patients with B-cell acute lymphoblastic leukemia. In contrast, due to the hostile immunosuppressive microenvironment of a solid tumor, CAR T-cell accessibility and survival continue to pose a considerable challenge, which leads to their limited therapeutic efficacy. In this study, we constructed two anti-MUC1 CAR-T cell lines. One set of CAR-T cells contained SM3 single chain variable fragment(sc Fv) sequence specifically targeting the MUC1 antigen and co-expressing interleukin(IL) 12(named SM3-CAR). The other CAR-T cell line carried the SM3 sc Fv sequence modified to improve its binding to MUC1 antigen(named p SM3-CAR) but did not co-express IL-12. When those two types of CAR-T cells were injected intratumorally into two independent metastatic lesions of the same MUC1+ seminal vesicle cancer patient as part of an interventional treatment strategy, the initial results indicated no side-effects of the MUC1 targeting CAR-T cell approach, and patient serum cytokines responses were positive. Further evaluation showed that p SM3-CAR effectively caused tumor necrosis, providing new options for improved CAR-T therapy in solid tumors. | Fengtao You Licui Jiang Bozhen Zhang Qiang Lu Qiao Zhou Xiaoyang Liao Hong Wu Kaiqi Du Youcai Zhu Huimin Meng Zhishu Gong Yunhui Zong Lei Huang Man Lu Jirong Tang Yafen Li Xiaochen Zhai Xiangling Wang Sisi Ye Dan Chen Lei Yuan Lin Qi Lin Yang | 2016 | Science China(Life Sciences)2016，59，4： | 16 |
| 5 | Application of gemstone spectral imaging for efficacy evaluation in hepatocellular carcinoma after transarterial chemoembolization显示文摘AIM: To assess the value of gemstone spectral imaging(GSI) in efficacy evaluation in hepatocellular cancer(HCC) after transcatheter arterial chemoembolization(TACE) treatment.METHODS: Thirty patients with HCC underwent GSI, including nonenhanced, arterial, portalvenous and delayed phase scans, after TACE treatment. Arterial phase images were acquired with GSI for reconstruction of virtual nonenhanced images and color overlay images. Digital subtraction angiography(DSA) was performed in all these patients. Two blinded and independent readers evaluated the data in two reading sessions; standard nonenhanced, arterial, portalvenous, and delayed phase images were read in session A, and the optimal monochromatic images, iodine/water based images and spectrum features were read in session B. Sensitivity and specificity were calculated with the DSA data as the reference standard. The sensitivity and specificity were compared using the χ2 test.RESULTS: DSA revealed 154 lesions in 30 patients, and 100 of them had blood supply. Overall sensitivity and specificity were 72%( 72/100) and 77.8 %(42/54) for session A, and 97%(97/100) and 94.4%(51/54) for session B, respectively. The sensitivity and specificity of the two reading sessions were significantly different(χ2 = 23.04, χ2 = 7.11, P < 0.05).CONCLUSION: Compared with conventional CT, GSI could significantly improve the detection of small and multiple lesions without increasing the radiation dose. Based on spectrum features, GSI could assess tumor homogeneity and more accurately identify residual tumors and recurrent or metastatic lesions during efficacy evaluation and follow-up in HCC after TACE treatment. | Qi-Yu Liu Chuan-Dong He Ying Zhou Dan Huang Hua Lin Zhong Wang Dong Wang Jin-Qiu Wang Li-Ping Liao | 2016 | World Journal of Gastroenterology2016，22，11： | 15 |
| 6 | PRRT2 deficiency induces paroxysmal kinesigenic dyskinesia by regulating synaptic transmission in cerebellum显示文摘在充满脯氨酸的 transmembrane 蛋白质的变化(PRRT2 ) 2 与发作性的 kinesigenic 运动障碍(PKD ) 和几另外的发作性的神经病学的疾病被联系,但是 PRRT2 功能和病原的机制仍然保持大部分阴暗。这里,我们证明 PRRT2 是与 SNARE 建筑群和 downregulates 的部件交往的 presynaptic 蛋白质它的形成。Loss-of-function 变异的老鼠显示出显型由小脑的概括发作,过高热,或 optogenetic 刺激触发了的象 PKD 一样。有在小脑的小粒房间(GC ) 的特定的 PRRT2 删除的变异的老鼠概括在 Prrt2 空的老鼠看见的行为的显型。而且,记录在显示出的小脑的片做了在短暂举起的 GC 结果的刺激由 Purkinje 房间开火的抑制跟随了的那 optogenetic。在 PKD 处理使用的 anticonvulsant 药 carbamazepine 也在变异的老鼠减轻了象 PKD 一样行为。一起,我们的调查结果作为 SNARE 建筑群的一个新奇管理者识别 PRRT2 并且提供位于 PRRT2 相关的行为下面的电路机制。 | Guo-He Tan Yuan-Yuan Liu Lu Wang Kui Li Ze-Qiang Zhang Hong-Fu Li Zhong-Fei Yang Yang Li Dan Li Ming-Yue Wu Chun-Lei Yu Juan-Juan Long Ren-Chao Chen Li-Xi Li Lu-Ping Yin Ji-Wei Liu Xue-Wen Cheng Qi Shen You-Sheng Shu Kenji Sakimura Lu-Jian Liao Zhi-Ying Wu Zhi-Qi Xiong | 2018 | Cell Research2018，28，1： | 14 |
| 7 | Left atrial appendage morphology in patients with atrial fibrillation in China: implications for stroke risk assessment from a single center study显示文摘 | Kong Bin Liu Yu Hu He Wang Lei Fan Yang Mei Yang Liu Wanli Liao Jiafen Liu Dan Xing Dong Huang He | 2014 | Chinese Medical Journal2014，，24： | 12 |
| 8 | 中国5地无偿献血者HCV流行特征分析显示文摘目的了解我国献血者丙型肝炎病毒(HCV)的初筛阳性率和确证阳性率。方法采用2种不同ELA试剂对2013年6月-2016年12月我国5家血液中心/中心血站献血者血液标本进行ALT、HBsAg、抗-HCV、抗-HIV、抗-TP进行常规筛查,抗-HCV单试剂或双试剂筛查阳性标本进一步采用MP HCV Blot 3. 0进行确证检测。分别估算首次献血者的HCV流行率及重复献血者的HCV发病率,并采用χ~2检验比较不同人群体、不同献血类型之间HCV确证阳性率。结果在1 276 544人次献血样本中,有3 855 (0. 30%)为抗-HCV ELISA初筛反应性,其中只有30. 18%(904/2 995,)确证为抗-HCV阳性。本次研究中,首次献血者总计648607人次,其中1080份样本确证为抗-HCV阳性,即首次献血者HCV流行率为166. 56 (156. 04—177. 08)/100 000人次,年龄、受教育水平、职业均与HCV确证阳性率相关。重复献血者总计627 937人次,其中126份样本确证为抗-HCV阳性,即重复献血者HCV发病率为13. 04(9. 55—17. 81)/100 000人次,其中重复献血者抗-HCV确证阳性率明显高于单采血小板献血者(0. 02%vs0. 01%)。结论本研究表明我国献血者HCV仍处于较高水平流行状态。尽管现在NAT已成为我国血液检测的常规检测项,但仍需持续有效地对我国输血HCV传播风险进行监测和控制。同时,新的规范中规定可采用1种或2种EIA进行筛查,本意是为了提高检测敏感性,但本研究中EIA筛查阳性样本如此低的确证阳性率提示其特异性差,可能会造成许多合格的献血者丢失。因此,优化献血者HCV筛查策略将有助于防止血液浪费。 | 付萍 Liao Dan 吕运来 张洪斌 何伟兰 黄梅 文秀琼 马红丽 何涛 柯玲 吴秉婷 刘鱼 何苗 Liu Jing Don Brambill 王憬惺 Paul Ness Shan Hua | 2018 | 中国输血杂志2018，31，9： | 10 |
| 9 | Interferon-c and interleukin-4 detected in serum and saliva from patients with oral lichen planus显示文摘Our previous salivary study had demonstrated an apparent T helper 2(Th2)-predominance in saliva of oral lichen planus(OLP) patients and suggested a potential of salivary interleukin-4(IL-4) as a biomarker for monitoring disease severity. To further determine the consistency of Th1/Th2 bias of OLP, this study investigated the expression profile of interferon-c(IFN-c) and IL-4 in serum and the relationship of the serum levels of these cytokines with their saliva partners. Sixty ethnic Chinese patients with OLP(40 of the erythematous/ulcerative form and 20 of the reticular form) were recruited for this study, with 40 age–sex-matched healthy volunteers as control group. IFN-c and IL-4 levels in serum and paired saliva samples were screened by enzyme-linked immunosorbent assay. OLP patient showed a low-level IFN-c but high-level IL-4 expression profile in both serum and saliva, with a lower IFN-c/IL-4 ratio. Serum IL-4 level in the erythematous/ulcerative group was significantly higher than that in the reticular group. Serum levels of IFN-c and IL-4 were significantly and positively correlated with their saliva partners. These results provided more evidence for Th2 cytokinepredominant immune imbalance in OLP, as well as the potential of IL-4 as the biomarker for monitoring severity of OLP. | Wen-Zhao Liu Ming-Jing He Long Long Dong-Liang Mu Ming-Shu Xu Xue Xing Xin Zeng Ga Liao Hong-Xia Dan Qian-Ming Chen | 2014 | International Journal of Oral Science2014，6，1： | 10 |
| 10 | Relationship between pre-TIPS hepatic hemodynamics and postoperative incidence of hepatic encephalopathy显示文摘BACKGROUND: Hepatic encephalopathy (HE) is one of the complications that have limited the effectiveness of transjugular intrahepatic portosystemic shunt (TIPS) most significantly. Up to the present, the predicting factors of HE post-TIPS have been debated controversially. This study was undertaken to verify the relationship between pre-TIPS intrahepatic hemodynamics and the incidence of post-TIPS HE. METHODS: The hepatic blood dynamics was evaluated in 41 patients with liver cirrhosis before TIPS and at one month after TIPS by ultrasonography. The patients were divided into two groups according to Doppler findings before TIPS: group 1, patients with prograde portal flow, and group 2, patients with hepatofugal or back-forth portal flow. The clinical characteristics (age, sex, etiology of liver disease, pre-TIPS Child-Pugh score, incidence of pre-TIPS HE, and portacaval pressure gradient), incidence of post TIPS HE, and pre-/post-TIPS hepatic arterial resistant index (RI) in the two groups were compared. The independent prognostic value of pre-TIPS variables for the onset of HE after TIPS, including age, Child-Pugh score, presence of HE before TIPS, and the pattern of portal flow, was tested with a multiple-factor regression analysis. RESULTS: No significant difference in age, etiology of liver disease, indications of TIPS placement, incidence of HE before TIPS, and portacaval gradient before and after TIPS was observed between the two groups; but liver failure was more severe in group 2 (P<0.05). The incidence of post-TIPS HE in group 2 was significantly lower than that in group 1 (P<0.01). Pre-TIPS, the RI of the hepatic artery in group 1 was significantly higher than that in group 2 (P<0.01). However, TIPS induced a significantly decreased RI in group 1 (P <0.01), but not in group 2. Multiple-factor regression analysis demonstrated that the pattern of portal flow before TIPS was closely associated with the onset of post TIPS HE. CONCLUSIONS: Pre-TIPS intrahepatic hetnodynamics is closely related to the incidence of post-TIPS HE. Hepatic hetnodynamics of patients with hepatofugal portal blood flow only changes a little after TIPS and still provides compensatory blood supply of the hepatic artery, and the hepatic function is less affected. Hence HE is unlikely. Hepatic hemodynamics of patients with prograde portal blood flow changes a lot after TIPS, and dual blood supply of the portal vein and hepatic artery changes into compensatory blood supply of the hepatic artery, and hepatic function suffers greatly in a short time. Thus HE is mostly likely. | Dan Deng, Ming-Song Liao, Jian-Ping Qin and Xiao-An Li Departments of Ultrasonography Gastroenterology General Hospital, Cheng- du Military Command, Chengdu 610083, China | 2006 | Hepatobiliary & Pancreatic Diseases International2006，5，2： | 9 |
| 11 | Macrophage migration inhibitory factor as a potential prognostic factor in gastric cancer显示文摘AIM:To investigate macrophage migration inhibitory factor(MIF) expression and its clinical relevance in gastric cancer,and effects of MIF knockdown on proliferation of gastric cancer cells. METHODS:Tissue microarray containing 117 samples of gastric cancer and adjacent non-cancer normal tissues was studied for MIF expression by immunohistochemistry(IHC) semiquantitatively,and the association of MIF expression with clinical parameters was analyzed. MIF expression in gastric cancer cell lines was detected by reverse transcriptionpolymerase chain reaction(RT-PCR) and Western blot. Two pairs of si RNA targeting the MIF gene(MIF si-1 and MIF si-2) and one pair of scrambled si RNA as a negative control(NC) were designed and chemically synthesized. All si RNAs were transiently transfected in AGS cells with OligofectamineTM to knock down the MIF expression,with the NC group and mock group(OligofectamineTM alone) as controls. At 24,48,and 72 h after transfection,MIF m RNA was analyzed by RTPCR,and MIF and proliferating cell nuclear antigen(PCNA) proteins were detected by Western blot.The proliferative rate of AGS cells was assessed by methylthiazolyl tetrazolium(MTT) assay and colony forming assay.RESULTS:The tissue microarray was informative for IHC staining,in which the MIF expression in gastric cancer tissues was higher than that in adjacent noncancer normal tissues(P < 0.001),and high level of MIF was related to poor tumor differentiation,advanced T stage,advanced tumor stage,lymph node metastasis,and poor patient survival(P < 0.05 for all). After si RNA transfection,MIF m RNA was measured by real-time PCR,and MIF protein and PCNA were assessed by Western blot analysis. We found that compared to the NC group and mock group,MIF expression was knocked down successfully in gastric cancer cells,and PCNA expression was downregulated with MIF knockdown as well. The cell counts and the doubling times were assayed by MTT 4 d after transfection,and colonies formed were assayed by colony forming assay 10 d after transfection; all these showed significant changes in gastric cancer cells transfected with specific si RNA compared with the control si RNA and mock groups(P < 0.001 for all).CONCLUSION:MIF could be of prognostic value in gastric cancer and might be a potential target for small-molecule therapy. | Long-Jun He Dan Xie Pin-Jin Hu Yi-Ji Liao Hai-Xia Deng Hsiang-Fu Kung Sen-Lin Zhu | 2015 | World Journal of Gastroenterology2015，21，34： | 9 |
| 12 | The effects and regulatory mechanism of RIP3 on RGC-5 necroptosis following elevated hydrostatic pressure显示文摘Necroptosis 是在各种各样的疾病被含有的调整房间死亡的一种类型。交往受体的蛋白质 3 (RIP3 ) , RIP 家庭的一个成员,是 necroptotic 小径的一个重要调停人。在由 caspase-8 的 Asp328 的 RIP3 的劈开废除 RIP3 的 kinase 活动,它为 necroptosis 是批评的。而且, RIP3 是显著地 upregulated 在尖锐高 intra 眼睛的压力和氧葡萄糖剥夺的早阶段期间。在这研究, RIP3 的效果在提高的静水力学的压力(EHP ) 期间被调查, caspase-8 通过调整了 RIP3 劈开的可能的机制被探索。流动 cytometry 分析表明导致 EHP 的坏死的网膜的中心房间 5 的数字(RGC-5 ) 房间被减少在以后 RIP3 击倒。而且,在正常 RGC-5 房间的 malondialdehyde (MDA ) 层次和肝糖 phosphorylase (PYGL ) 活动比在在 EHP 以后的 RIP3 击倒的房间的那些高得多。导致 EHP 的 RGC-5 坏死显著地与 butylated hydroxyanisole (BHA ) 在处理以后被减少,反应的氧种类(ROS ) scavenger。MDA 层次和 PYGL 活动比在有在 EHP 以后的 caspase-8 抑制的房间的那些在正常 RGC-5 房间是更低的。西方的污点分析证明 RIP3 劈开产品是在有 caspase-8 抑制的房间的 upregulated。另外,流动 cytometry 分析表明导致 EHP 的坏死的 RGC-5 房间的数字在 caspase-8 抑制以后被增加。我们的结果建议 RGC-5 necroptosis 追随者 EHP 被 RIP3 通过 PYGL 活动和随后的 ROS 累积的正式就职调停。因此, caspase-8 可以经由 RIP3 劈开参予 RGC-5 necroptosis 的规定。 | Lei Shang Wei Ding Na Li Lvshuang Liao Dan Chen Jufang Huang Kun Xiong | 2017 | Acta Biochimica et Biophysica Sinica2017，49，2： | 9 |
| 13 | Honokiol:a promising small molecular weight natural agent for the growth inhibition of oral squamous cell carcinoma cells显示文摘Honokiol (HNK) is a small organic molecule purified from magnolia species and has demonstrated anticancer activities in a variety of cancer cell lines; however, its effect on oral squamous cell carcinoma (OSCC) cells is unknown. We investigated the antitumor activities of HNK on OSCC cells in vitro for the first time. The inhibitory effects of HNK on the growth and proliferation of OSCC cells were demonstrated via in vitro 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and propidium iodide (PI) assays, and the apoptotic cells were investigated by the observation of morphological changes and detection of DNA fragmentation via PI, TdT-mediated dUTP-biotin nick end labeling (TUNEL), and DNA ladder assays, as well as flow cytometry assay. The results showed that HNK inhibited the growth and proliferation of OSCC cells in vitro in a time and dose-dependent manner. The inhibitory effect was associated with the cell apoptosis induced by HNK, evidenced by the morphological features of apoptotic cells, TUNEL-positive cells and a degradation of chromosomal DNA into small internucleosomal fragments. The study also demonstrated here that the inhibition or apoptosis mediated by 15 μg·mL-1 or 20 μg·mL-1 of HNK were more stronger compared with those of 20 μg·mL-1 5-fluorouracil (5-Fu, the control) applied to OSCC cells, when the ratio of OSCC cell numbers were measured between the treatment of different concentrations of HNK to the 5-Fu treatment for 48 h. HNK is a promising compound that can be potentially used as a novel treatment agent for human OSCC. | Xi-rui Chen Rui Lu Hong-xia Dan Ga Liao Min Zhou Xiao-yu Li Ning Ji | 2011 | International Journal of Oral Science2011，3，1： | 8 |
| 14 | Resolvin-D1 inhibits interleukin-8 and hydrogen peroxide production induced by cigarette smoke extract in 16HBE cells via attenuating NF-κB activation显示文摘 | Dong Jiajia Zhang Mingke Liao Zenglin Wu Wei Wang Tao Chen Lei Yang Ting Guo Lingli Xu Dan Wen Fuqiang | 2014 | Chinese Medical Journal2014，，3： | 6 |
| 15 | Enhanced Desorption of PAHs from Manufactured Gas Plant Soils Using Different Types of Surfactants显示文摘Surfactant enhanced remediation is thought to be an effective method for the remediation of soils polluted with hydrophobic organic compounds. Desorption of polycyclic aromatic hydrocarbons(PAHs) from an abandoned manufactured gas plant(MGP) soil was evaluated using four eluting agents including Triton X-100(TX100),sodium dodecylbenzene sulfonate(SDBS),rhamnolipid water solution(RWS) and rhamnolipid fermentation broth(RFB). The weight solubilization ratios for acenaphthene and fluorene were in the order of TX100 > SDBS > RWS > RFB. The Sm value,which indicates the maximum amounts of surfactants adsorbed in the soil,was in the order of RWS > RFB > SDBS > TX100. By using 8 g L-1of TX100,SDBS and RWS and 100% of RFB,the T-PAHs removal for the MGP soil contaminated with 207.86 mg T-PAHs kg-1 dry soil was 48.0%,45.7%,1.9%,and 8.6%,respectively,while that decreased to 41.6%,37%,0.38%,and 1.3% for the soil contaminated with 3 494.78 mg T-PAHs kg-1 dry soil. Only 8 g L-1 TX100 could remove all types of the 16 PAHs partly in the MGP soil,and the removal efficiencies of different PAHs ranged from 13% to 77.8%. The results of this study herein provide valuable information for the selection of TX100 surfactant for remediating PAH-contaminated soils in MGP. | CHONG Zhong-Yi LIAO Xiao-Yong YAN Xiu-Lan SUN Lu ZHAO Dan LIANG Tao | 2014 | Pedosphere2014，24，2： | 6 |
| 16 | Spatial and temporal patterns of amphibian species richness on Tianping Mountain,Hunan Province,China显示文摘DEAR EDITOR,Exploring species richness patterns across space and time can help in understanding species distribution and in formulating conservation strategies.Among taxa,amphibians are of utmost importance as they are highly sensitive to environmental changes due to their unique life histories(Zhong et al.,2018).Here,we investigated the spatial and temporal patterns of amphibian species richness on Tianping Mountain in China.Specifically,we established 10 transects at low to high elevations,and sampled amphibians in April,June,August,and October 2017.Our results demonstrated that amphibian species composition and richness varied significantly at both spatial and temporal scales and were associated with gradients of environmental change in microhabitats on Tianping Mountain. | Wen-Bo Zhu Chun-Lin Zhao Chun-Lin Liao Bei Zou Dan Xu Wei Zhu Tian Zhao Jian-Ping Jiang | 2020 | Zoological Research2020，41，2： | 5 |
| 17 | The protective effects of Polygonum multiflorum stilbeneglycoside preconditioning in an ischemia/reperfusion model of HUVECs显示文摘 | Li-ping LIU Zhang-ping LIAO Dong YIN Wei-dong LI Dan LIU Qing LI Qi-ren HUANG Yao-fang YANG Ming HE | 2010 | Acta Pharmacologica Sinica2010，31，4： | 5 |
| 18 | Phaeocystis globosa Bloom Monitoring: Based on P. globosa Induced Seawater Viscosity Modification Adjacent to a Nuclear Power Plant in Qinzhou Bay, China显示文摘The mucus produced by the outbreak of Phaeocystis globosa in the adjacent waters of the Fangchenggang Nuclear Power Plant(FCGNPP) in China has blocked the entrance of the cooling water filtration system of the FCGNPP, and posed a threat to the safe operation of the FCGNPP. At present, there is no related research on whether the changes in seawater viscosity could be used as a new method for monitoring and providing early warning of P. globosa tide. During a complete red tide cycle, the temporal and spatial changes in the hydrological conditions(temperature, salinity, dissolved oxygen), chlorophyll-a(Chl-a), composition and abundance of phytoplankton, number and size of P. globose colonies, concentration of transparent exopolymer particles(TEP) and the seawater viscosity were measured in this study. The results indicate that there was an extremely significant negative correlation between the physical seawater viscosity η_(T,S) and temperature, and the correlation coefficient reached-0.998. The biological seawater viscosity η_(Bio) was positively correlated with the number of P. globosa colonies, and the correlation coefficients is 0.747. Because the increase in phytoplankton abundance, especially during the outbreak of P. globosa and a large amount of mucus produced by the colonies could significantly increase seawater viscosity, we suggest that biological factors were the main reasons for the increase in seawater viscosity. The η_(Bio) was completely consistent with the occurrence process of P. globosa bloom and could be used as a valuable index for P. globosa bloom monitoring. | KANG Zhenjun YANG Bin LAI Junxiang NING Yi ZHONG Qiuping LU Dongliang LIAO Riquan WANG Pei Solomon Felix Dan SHE Zhicai JIA Zhen LAO Yanling LI Nan | 2020 | Journal of Ocean University of China2020，19，5： | 5 |
| 19 | Mixed lineage kinase domain-like protein induces RGC-5 necroptosis following elevated hydrostatic pressure显示文摘 | Lvshuang Liao Lei Shang Na Li Shuchao Wang Mi Wang Yanxia Huang Dan Chen Jufang Huang Kun Xiong | 2017 | Acta Biochimica et Biophysica Sinica2017，49，10： | 5 |
| 20 | Effect of Baizhu(Rhizoma Atractylodis Macrocephalae)extract on intestinal absorption of brucine and strychnine in vitro and in situ显示文摘OBJECTIVE:To investigate the antagonistic effect of the extract of Baizhu(Rhizoma Atractylodis Macrocephalae)(RAM)on the intestinal absorption of brucine and strychnine in Strychnos nux-vomica(NUX)and propose the mechanism of these effects.METHODS:The apparent permeability value(Papp)and absorption rate constant(Ka)were chosen as indices.The everted intestinal sac model and in situ single-pass intestinal perfusion model were used to study the effects of the RAM extract on the absorption of brucine and strychnine.To confirm the results,the brucine and strychnine concentrations in hepatic portal venous blood were determined.Western blotting was used to study P-glycoprotein(P-gp)expression in the Caco-2 cell line.RESULTS:Papp and Ka of brucine and strychnine were significantly increased in the presence of a P-gp inhibitor,but no significant increase was noted in the presence of a tight junction regulator.The RAM extract inhibited the absorption of brucine and strychnine and enhanced P-gp expression.CONCLUSION:The primary absorption mechanism for brucine and strychnine is passive transport,which is affected by P-gp. | Lu Dan Jiang Qieying Zhang Jing Zeng Ronggui Liao Zhenggen Liang Xinli | 2020 | Journal of Traditional Chinese Medicine2020，40，4： | 4 |
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