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| 1 | Correction of a genetic disease by CRISPR-Cas9-mediated gene editing in mouse spermatogonial stem cells显示文摘Spermatogonial 干细胞(SSC ) 能在移植以后生产众多的男配偶子进接受者睾丸,介绍为基因治疗和修改基因的动物的连续生产的一条珍贵途径。然而, SSC 的成功的基因操作被限制了,部分由于复杂性和当前可得到的基因编辑技术的低效率。这里,我们证明有效基因修正能用 CRISPR-Cas9 系统被介绍进 SSC。我们使用了 CRISPR-Cas9 系统变异在 SCC 的 EGFP transgene 或内长的 Crygc 基因。变异的 SSC 在移植以后经历了精子发生进不肥沃的老鼠睾丸的生精的小管。圆 spermatids 被产生并且在进成熟卵母细胞的注射以后,支持了显示相应变异的显型的异质接合的后代的生产。而且,在 Crygc 的一个引起疾病的变化(在 SSC 先存在的 Crygc −/−) 能乐意地到加入的 CRISPR-Cas9-induced nonhomologous 结束(NHEJ ) 或指导相同的修理(HDR ) 被修理,导致没有是的离开目标修正的证据带改正的基因的 SSC 线由整个染色体的定序出现。用从这些线产生的圆 spermatids 的授精在 100% 的效率与改正的显型产生了后代。我们的结果表明有效基因由 CRISPR-Cas9 系统在老鼠 SSC 编辑,并且提供在 SSC 经由基因修正治好基因疾病的原则的证明。 | Yuxuan Wu Hai Zhou Xiaoying Fan Ying Zhang Man Zhang Yinghua Wang Zhenfei Xie Meizhu Bai Qi Yin Dan Liang Wei Tang Jiaoyang Liao Chikai Zhou Wujuan Liu Ping Zhu Hongshan Guo Hong Pan Chunlian Wu Huijuan Shi Ligang Wu Fuchou Tang Jinsong Li | 2015 | Cell Research2015，25，1： | 37 |
| 2 | DNA methylation and chromatin accessibility profiling of mouse and human fetal germ cells显示文摘染色质改变为人的初发的细菌房间的 epigenetic reprogramming 是重要的。然而,全面染色质状态还没为人的胎儿的细菌房间(FGC ) 被分析了。这里,我们使用 nucleosome 占有和 methylation 定序方法在人和老鼠在胎儿的细菌房间开发期间在一系列关键时间点分析染色体宽的染色质可接近性和 DNA methylome。我们在人和老鼠 FGC 发现 116 887 和 137 557 个弄空 nucleosome 的区域(NDR ) ,盖住 germline 特定、高度有活力的规章的 genomic 元素的一个大集合,例如 enhancers。而且,我们发现远侧的 NDR 在人的 FGC 为象 NANOG , SOX17 , AP2 和 OCT4 那样的 pluripotency 和细菌房间主人管理者的有约束力的主题明确地被充实,显示在pluripotency相关的基因和细菌之间的精细的规章的平衡的存在在人的 FGC 的房间特定的基因,并且为在 vivo 的细菌房间开发的这些基因的功能的意义。我们的工作为把提供一张全面、高分辨率的路线图染色质状态转变动力学在人和老鼠 FGC 的 epigenomic reprogramming 期间。 | Hongshan Guo Boqiang Hu Liying Yan Jun Yong Yan Wu Yun Gao Fan Guo Yu Hou Xiaoying Fan Ji Dong Xiaoye Wang Xiaohui Zhu Jie Yan Yuan Wei Hongyan Jin Wenxin Zhang Lu Wen FuchouTang Jie Qiao | 2017 | Cell Research2017，27，2： | 9 |
| 3 | H3K4me3 epigenomic landscape derived from ChIP-Seq of 1000 mouse early embryonic cells显示文摘 | Jie Shen Dongqing Jiang Yusi Fu Xinglong Wu Hongshan Guo Binxiao Feng Yuhong Pang Aaron M Streets Fuchou Tang Yanyi Huang | 2015 | Cell Research2015，25，1： | 4 |
| 4 | Homeobox B8 Targets Sterile Alpha Motif Domain-Containing Protein 9 and Drives Glioma Progression显示文摘Gliomas are the most commonly occurring tumors of the central nervous system. Glioblastoma multiforme (GBM) is the most malignant and aggressive brain cancer in adults. Further understanding of the mechanisms underlying the aggressive nature of GBM is urgently needed. Here we identified homeobox B8(HOXB8), a member of the homeobox family, as a crucial contributor to the aggressiveness of GBM. Data mining of publicly accessible RNA sequence datasets and our patient cohorts confirmed a higher expression of HOXB8 in the tumor tissue of GBM patients, and a strong positive correlation between the expression level and pathological grading of tumors and a negative correlation between the expression level and the overall survival rate. We next showed that HOXB8 promotes the proliferation and migration of glioblastoma cells and is crucial for the activation of the PI3K/AKT pathway and expression of epithelial–mesenchymal transition-related genes, possibly through direct binding to the promoter of SAMD9 (Sterile Alpha Motif Domain-Containing Protein 9) and activating its transcription. Collectively, we identified HOXB8 as a critical contributor to the aggressiveness of GBM, which provides insights into a potential therapeutic target for GBM and opens new avenues for improving its treatment outcome. | Wenping Ma Hongze Jin Wenjie Liu Xiaojuan Li Xingang Zhou Xinwu Guo Runfa Tian Qi Cui Junjie Luo Yueying Jiao Youtao Yu Haifeng Yang Hongshan Zhao | 2020 | Neuroscience Bulletin2020，36，4： | 2 |
| 5 | Polydentate-ligand-supported self-assembly of heterometallic T-shaped Co_4 Dy cluster showing slow magnetic relaxation显示文摘A novel mixed-valent heterometallic pentanuclear CoⅢ3 CoⅡ Dy Ⅲ cluster has been rationally assembled taking advantage of a bifunctional ligand with o-vanillin and tripodal tris(hydroxymethyl)aminomethane units. This unique heterometallic cluster represents a rare example of T-shaped molecules exhibiting slow magnetic relaxation. | KE HongShan GUO Yang ZHAO Lang TANG JinKui | 2012 | Science China Chemistry2012，55，6： | 1 |
| 6 | Purification and characterization of Ginsenoside-β-glucosidase显示文摘 | MA Xiaoqun GUO Yong | 1999 | J Ginseng Res1999，23，1： | 1 |
| 7 | High intratumoral expression of elF4A1 promotes epithelial-to-mesenchymal transition and predicts unfavorable prognosis in gastric cancer显示文摘Gastric cancer is an important health problem,being the fifth most common cancer and the third leading cause of cancer-related death worldwide.Aberrant protein translation contributes to the oncogenesis and development of cancers,and upregulation of translation initiation factor elF4A1 has been observed in several kinds of malignancies.However,the role of elF4A1 in gastric cancer progression remains unclear.In this study,we found that the expression of elF4A1,a component of translation initiation complex,was increased in gastric cancer.High expression of elF4A1 was positively associated with poor tumor differentiation,late T stage,lymph node metastasis,advanced TNM stage,and poor prognosis in patients with gastric cancer.Overexpression of elF4A1 promoted the migration and invasion of gastric cancer cells in vitro and enhanced tumor metastasis in nude mice model.Mechanism studies revealed that elF4A1 induced epithelial-to-mesenchymal transition(EMT)of gastric cancer cells through driving the translation of SNAI1 mRNA.Together,these findings indicate that elF4A1 promotes EMT and metastasis of gastric cancer and suggest that elF4A1 is a potential target for the adjuvant therapy for gastric cancer patients. | Chanchan Gao Xinyin Guo Anwei Xue Yuanyuan Ruan Hongshan Wang Xiaodong Gao | 2020 | Acta Biochimica et Biophysica Sinica2020，52，3： | 1 |
| 8 | Purification and characterization of ginsenoside-β-glucosidase显示文摘 | YU Hongshan MA Xiaoqun GUO Yong | 1999 | J Ginseng Res1999，23，1： | 1 |
| 9 | Purification and characterization of ginsenoside-β-glucosidase 显示文摘 | YU Hongshan MA Xiaoqun GUO Yong | 1999 | Ginseng Res1999，23，1： | 1 |
| 10 | Purification and characterization of ginsenoside-β-glucosidase显示文摘 | MA Xiaoqun GUO Yong | 1999 | J Ginseng Res1999，23，1： | 1 |
| 11 | Purification and characterization of gisenoside β-glucosidase显示文摘 | YU Hongshan WU Shoujie GUO Yong | 1999 | J Ginseng Res1999，23，1： | 1 |
| 12 | Polydentate-ligand-supported self-assembly of heterometallic T-shaped Co_4Dy cluster showing slow magnetic relaxation显示文摘A novel mixed-valent heterometallic pentanuclear CoIII3CoIIDyIII cluster has been rationally assembled taking advantage of a bifunctional ligand with o-vanillin and tripodal tris(hydroxymethyl)aminomethane units. This unique heterometallic cluster represents a rare example of T-shaped molecules exhibiting slow magnetic relaxation. | KE HongShan GUO Yang ZHAO Lang TANG JinKui | 2012 | 中国科学:化学2012，42，6： | 0 |
| 13 | Charm and Beauty——A Comparison of Two Aesthetic Categories in Chinese and Western Paintings显示文摘Aesthetics in China is different from that in the West which is centered on the logical approach to the concept of 'beauty,' and is highly typified by chewing, tasting, and pondering, a process full of intuition and contemplation. Instead of 'beauty,' 'charm' is the central category in Chinese painting, presenting a different aesthetic system from that in the western world. | Wei Hongshan Guo Li | 2018 | Contemporary Social Sciences2018，3，2： | 0 |
| 14 | A new member of Sphenopsida,Neolobatannularia gen. nov. from Late Triassic of western Liaoning,China显示文摘A new genus Neolobatannularia Sun et Li gen. nov. with the type species Neolobatannularia liaoningensis Sun et Li sp. nov. from the Upper Triassic Yangcaogou Formation of Beipiao, Liaoning, China, is described in this paper. The new taxon is attributed to Equisetales of Sphenopsida based on its branch with internodes, node and leaves borne in whorls on nodes. It is a new member of the Mesozoic Sphenopsida. Although the new taxon shares some morphological characters with the genus Lobatannularia Kawasaki, a genus widely distributed in the Permian Cathaysia flora in East Asia, and the genus Lobatannulariopsis Yang from the Late Triassic of southwestern China, its unique branching pattern is markedly distinguished from the two known genera. Some Triassic known species of Lobatannularia may belong to the present new genus Neolobatannularia. | WANG Junyou LI Tao LIU Zhiping GUO Bin KANG Ai NA Yuling LI Yunfeng WANG Hongshan BO Junchen SUN Chunlin | 2019 | Global Geology2019，22，1： | 0 |
| 15 | Desalted duck egg white nanogels as Pickering stabilizers for food-grade oil-in-water emulsion显示文摘Achieving the reuse of traditional egg by-products,salted duck egg whites(SEW),is an urgent problem to be solved.In this current work,we constructed a heat-induced gel-assisted desalination method for SEW.Subsequently,a top-down way was utilized to prepare desalted duck egg protein nanogels(DEPN)with uniformly distributed diameters and their application in the oil/water(O/W)interface system was explored.The results revealed that the increase of DEPN concentration could lower the droplet size,however,the size was negatively correlated with the oil phase fraction.Moreover,the effect of pH,ionic strength,and temperature on the emulsion stability demonstrated that the DEPN-stabilized emulsion displayed superior physical stability under different conditions.The addition of NaCl resulted in the significant decrease in droplet size of the emulsion,while further increasing the NaCl concentration,the droplet size did not decrease accordingly.Besides,heat-treatment and cold-treatment had little negative effect on the stability of the emulsion.Even if the droplet size of the emulsion increased at 80℃for 3 h,the morphology of the emulsion remained unchanged.Our study demonstrated DEPN had great potential as a stabilizer for food-grade Pickering emulsions. | Jingyun Zhao Xiaohan Guo Ze Chen Yalei Dai Hongshan Liang Qianchun Deng Shugang Li Bin Zhou | 2022 | Food Science and Human Wellness2022，11，5： | 0 |
| 16 | 单细胞转录组分析揭示人类生殖系细胞与性腺微环境细胞之间的相互作用显示文摘文章简介人类胚胎生殖细胞(FGC,fetal germ cell)是精子和卵细胞的前体细胞,其正常发育对于物种稳定延续至关重要。然而,由于实验材料稀少以及缺少已知的特异性表面分选标志等原因,人们对于人类胚胎生殖细胞的发育进程知之甚少。 | Li Li Ji Dong Liying Yan Jun Yong Xixi Liu Yuqiong Hu Xiaoying Fan Xinglong Wu Hongshan Guo Xiaoye Wang Xiaohui Zhu Rong Li Jie Yan Yuan Wei Yangyu Zhao Wei Wang Yixin Ren Peng Yuan Zhiqiang Yan Boqiang Hu Fan Guo Lu Wen 汤富酬 乔杰 | 2018 | 科学新闻2018，0，4： | 0 |
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