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| 1 | Layer-by-layer deposition of bioactive layers on magnesium alloy stent materials to improve corrosion resistance and biocompatibility显示文摘Magnesium alloy is considered as one of the ideal cardiovascular stent materials owing to its good mechanical properties and biodegradability.However,the in vivo rapid degradation rate and the insufficient biocompatibility restrict its clinical applications.In this study,the magnesium alloy(AZ31B)was modified by combining the surface chemical treatment and in-situ self-assembly of 16-phosphonyl-hexadecanoic acid,followed by the immobilization of chitosan-functionalized graphene oxide(GOCS).Heparin(Hep)and GOCS were alternatively immobilized on the GOCS-modified surface through layer by layer(LBL)to construct the GOCS/Hep bioactive multilayer coating,and the corrosion resistance and biocompatibility were extensively explored.The results showed that the GOCS/Hep bioactive multilayer coating can endow magnesium alloys with an excellent in vitro corrosion resistance.The GOCS/Hep multilayer coating can significantly reduce the hemolysis rate and the platelet adhesion and activation,resulting in an excellent blood compatibility.In addition,the multilayer coating can not only enhance the adhesion and proliferation of the endothelial cells,but also promote the vascular endothelial growth factor(VEGF)and nitric oxide(NO)expression of the attached endothelial cells on the surfaces.Therefore,the method of the present study can be used to simultaneously control the corrosion resistance and improve the biocompatibility of the magnesium alloys,which is expected to promote the application of magnesium alloys in biomaterials or medical devices,especially cardiovascular stent. | Fan Gao Youdong Hu Guicai Li Sen Liu Li Quan Zhongmei Yang Yanchun Wei Changjiang Pan | 2020 | Bioactive Materials2020，5，3： | 12 |
| 2 | A transgene-free method for rapid and efficient generation of precisely edited pigs without monoclonal selection显示文摘Gene-edited pigs for agricultural and biomedical applications are typically generated using somatic cell nuclear transfer(SCNT).However, SCNT requires the use of monoclonal cells as donors, and the time-consuming and laborious monoclonal selection process limits the production of large populations of gene-edited animals. Here, we developed a rapid and efficient method named RE-DSRNP(reporter RNA enriched dual-sg RNA/CRISPR-Cas9 ribonucleoproteins) for generating gene-edited donor cells. RE-DSRNP takes advantage of the precise and efficient editing features of dual-sg RNA and the high editing efficiency, low off-target effects, transgene-free nature, and low cytotoxic characteristics of reporter RNA enriched RNPs(CRISPR-Cas9ribonucleoproteins), thus eliminating the need for the selection of monoclonal cells and thereby greatly reducing the generation time of donor cells from 3–4 weeks to 1 week, while also reducing the extent of apoptosis and chromosomal aneuploidy of donor cells. We applied RE-DSRNP to produce cloned pigs bearing a deletion edit of the wild-type p53-induced phosphatase 1(WIP1)gene: among 32 weaned cloned pigs, 31(97%) carried WIP1 edits, and 15(47%) were homozygous for the designed fragment deletion, and no off-target event was detected. The WIP1 knockout(KO) pigs exhibited male reproductive disorders, illustrating the utility of RE-DSRNP for rapidly generating precisely edited animals for functional genomics and disease research. REDSRNP's strong editing performance in a large animal and its marked reduction in the required time for producing SCNT donor cells support its application prospects for rapidly generating populations of transgene-free cloned animals. | Kui Xu Xiuling Zhang Zhiguo Liu Jinxue Ruan Changjiang Xu Jingjing Che Ziyao Fan Yulian Mu Kui Li | 2022 | Science China(Life Sciences)2022，65，8： | 9 |
| 3 | The noble gas isotope geochemical composition of chert at the bottom of Cambrian in Tarim Basin,China显示文摘In the Tarim Basin,black shale series at the bottom of Cambrian is one of the important marine facies hydrocarbon source rocks. This research focuses on the analysis of the isotope of noble gas of 11 cherts. The R/Ra ratio of chert in the Keping area is 0.032―0.319,and 40Ar/36Ar is 338―430. In Quruqtagh the R/Ra ratio is 0.44―10.21,and 40Ar/36Ar is 360―765. The R/Ra ratio of chert increases with 40Ar/36Ar from the west to the east accordingly. They have evolved from the crust source area to the mantle source area in a direct proportion. Surplus argon 40ArE in chert is in direct proportion to the R/Ra ratio,indicating that it has the same origin of excess argon as in fluid and mantle source helium. Comparison of the R/Ra ratios between the west and the east shows that the chert in the eastern part formed from the activity system of the bottom hydrothermal venting driven by the mantle source,where the material and energy of crust and mantle had a strong interaction in exchange;whereas in the western part,chert deposited from the floating of hydrothermal plume undersea bottom,which is far away from the centre of activities of the hydrothermal fluid of ocean bottom. In addition,from noble gas isotope composition of chert,it is suggested that the ocean anoxia incident happened at the black shale of the Cambrian bottom probably because of the large-scaled ocean volcanoes and the followinghydrothermal activities. | SUN ShengLi1,CHEN JianFa2,3,ZHENG JianJing4 & LIU WenHui4 1 Monitoring Center for Marine Resource and Environment,Guangdong Ocean University,Zhanjiang 524088,China 2 Department of Geochemistry,Changjiang University,Jingzhou 434023,China 3 College of Resource and Information,Petroleum University,Beijing 102249,China 4 Lanzhou Institute of Geology,Chinese Academy of Sciences,Lanzhou 730000,China | 2009 | Science China Earth Sciences2009，52，S1： | 6 |
| 4 | Plant crop remains from the outer burial pit of the Han Yangling Mausoleum and their significance to Early Western Han agriculture显示文摘A large amount of carbonized plant remains were discovered in one of the outer burial pits of the Han Yangling Mausoleum, which was built more than 2000 years ago for the Jing Emperor, Liu, Qi (188―141 cal a BC), the fourth emperor of the Western Han Dynasty. The remains are identified by phytolith analysis and macrofossil morphological features. Seeds from foxtail millet (Setaria italica), broomcorn millet (Panicum miliaceum), rice (Oryza sativa) and chenopod (possible Chenopodium giganteum) are identified, suggesting that these four crops might have been the staple plant foods in the capital area (Guanzhong area), Shaanxi Province during the Early Western Han Dynasty. Chenopods were often considered as weeds since they have only been rarely found as carbonized seeds in prehistoric sites. This is the first time such a large amount of seeds has been found at a site, which provides strong material evidence for chenopod cultivation with a long history in China. Wheat was thought to be promoted and popularized in the Guanzhong area since the Wu Emporor, Liu, Che (156―87 cal a BC), the fifth emperor of the Western Han Dynasty. No wheat was found at this site, which supports the historical document record that wheat was still secondary in the diet and agrarian economy before the Wu Emperor's reign. | YANG XiaoYan LIU ChangJiang ZHANG JianPing YANG WuZhan ZHANG XiaoHu LV HouYuan | 2009 | Chinese Science Bulletin2009，54，10： | 5 |
| 5 | Injectable conductive gelatin methacrylate/oxidized dextran hydrogel encapsulating umbilical cord mesenchymal stem cells for myocardial infarction treatment显示文摘Umbilical cord mesenchymal stem cells(UCMSCs)transplantation has been proposed as a promising treatment modality for myocardial infarction(MI),but the low retention rate remains a considerable challenge.Injectable natural polymer hydrogels with conductivity ability are highly desirable as cell delivery vehicles to repair infarct myocardium and restore the cardiac function.In this work,we developed a hydrogel system based on gelatin methacrylate(GelMA)and oxidized dextran(ODEX)as cell delivery vehicles for MI.And dopamine could be used as a reductant of graphene oxide(GO)to form reductive GO(rGO).By adjusting the amount of rGO,the conductivity of hydrogels with 0.5 mg/mL rGO concentration(≈10^(-4)S/cm)was similar to that of natural heart tissue.In vitro cell experiments showed that the prepared hydrogels had excellent biocompatibility and cell delivery ability of UCMSCs.More importantly,GelMA-O5/rGO hydrogel could promote UCMSCs growth and proliferation,improve the myocardial differentiation ability of UCMSCs,and up-regulate the expression of cTnI and Cx43.Further in vivo experiments demonstrated that GelMA-O5/rGO/UCMSCs Hydrogel could significantly improve the ejection fraction(EF)of rats and significantly reduce myocardial infarct area compared to PBS group,promote the survival of UCMSCs,enhance the expression level of cTnI and Cx43,and decrease the expression level of caspase-3.The findings of this study suggested that the injectable conductive GelMA-O5/rGO hydrogel encapsulating UCMSCs could improve damaged myocardial tissue and reconstruct myocardial function,which will be a promising therapeutic strategy for cardiac repair. | Shuoji Zhu Changjiang Yu Nanbo Liu Mingyi Zhao Zerui Chen Jian Liu Ge Li Huanlei Huang Huiming Guo Tucheng Sun Jimei Chen Jian Zhuang Ping Zhu | 2022 | Bioactive Materials2022，7，7： | 4 |
| 6 | Analytical and numerical studies on the nonlinear dynamic response of orthotropic membranes under impact load显示文摘Orthotropic membrane components and structures are widely used in building structures, instruments and meters, electronic engineering, space and aeronautics, etc., because of their light weights. However, the same lightweight combined with low stiffness make membranes prone to vibration under dynamic loads, and in some cases the vibration may lead to structural failure. Herein, the undamped nonlinear vibration response of pretension rectangular orthotropic membrane structures subjected to impact loading is studied by analytical and numerical methods. The analytical solution is obtained by solving the governing equations by the Bubnov-Galerkin method and the Lindstedt-Poincaré perturbation method. Numerical analysis has also been carried out based on the same theoretical model. The analytical and numerical results have been compared and analyzed, and the infl uence of various model parameters on membrane vibration discussed. The results obtained herein provide some theoretical basis for the vibration control and dynamic design of orthotropic membrane components and structures. | Liu Changjiang Zheng Zhoulian Yang Xiaoyan | 2016 | Earthquake Engineering and Engineering Vibration2016，15，4： | 4 |
| 7 | Comprehensive evaluation of Actinidia arguta fruit based on the nutrition and taste:67 germplasm native to Northeast China显示文摘Actinidia arguta is a berry fruit with high nutritional values.To evaluate the comprehensive quality,the fruit weight,titratable acid(TAC),soluble solids(SSC),vitamin C,mineral elements,and taste value of67 accessions of A.arguta were measured,and sensory attributes including olfactive intensity,juiciness,sweetness,sourness,and astringency were assessed.Cluster analysis was carried out based on mineral elements,and principal component analysis(PCA)was used for comprehensive score ranking.The result demonstrated that fruit weight ranged from 3.5 g to 24.2 g,SSC ranged from 11.94%to 20.56%,TAC ranged from 0.15%to 1.20%,and vitamin C ranged from 3.51 g/kg to 230.76 g/kg(fresh weight)among the tested samples.There were significant differences in sourness,sweetness,bitterness,and umami among the 67 accessions based on electronic tongue analysis,and this result was rapidly and objectively characterized by the sensory assessment.The dendrogram was obtained and clustered the accessions into two major clusters,and five principal components were extracted by PCA.Overall,the information provided by this study identified the 10 best germplasm resources for fresh-eating and processing fruits.It can provide theoretical reference for nutritional efficacy evaluation and food development of A.arguta. | Mengting Song Heran Xu Guang Xin Changjiang Liu Xiaorong Sun Yinhong Zhi Bin Li Yixiao Shen | 2022 | Food Science and Human Wellness2022，11，2： | 3 |
| 8 | An important military city of the Early Western Zhou Dynasty:Archaeobotanical evidence from the Chenzhuang site,Gaoqing,Shandong Province显示文摘In ancient Chinese history,the Western Zhou Dynasty has long been renowned for having expanded its control and territory into many other states.However,historical documents and archaeological records of this period are limited;thus the early history of the Western Zhou Dynasty's operation in eastern China and its establishment of the Qi and Lu states have been unclear.The discovery of the Chenzhuang city site in Gaoqing County,Shandong Province,with chariot-horse pits,an altar and bronze vessels with inscriptions,adds a new line of evidence for studying the history of this period.However,with no direct evidence,the nature of the city site is controversial.Plant remains,especially a large number of sweet clover seeds,recovered from this site by systematic archaeobotanical methods provide an important source of information for research into the site's function.Considering that modern sweet clover is superior fodder for horses and the sweet clover seeds from the Chenzhuang site coexist with chariot-horse pits and horse remains,it is suggested these sweet clover seeds might represent the fodder of battle steeds.This suggestion supports the opinion of those who believe the Chenzhuang city site was once an important military city of the Western Zhou Dynasty in eastern China. | JIN GuiYun ZHENG TongXiu LIU ChangJiang WANG ChuanMing GAO MingKui | 2012 | Chinese Science Bulletin2012，57，2： | 3 |
| 9 | Regulation of drug sensitivity of gastric cancer cells by human calcyclin-binding protein (CacyBP)显示文摘 | Yongquan Shi Wenhua Hu Fang Yin Li Sun Changjiang Liu Mei Lan Daiming Fan | 2004 | Gastric Cancer2004，，3： | 3 |
| 10 | Bicontinuous porous membranes with micro-nano composite structure using a facile atomization-assisted nonsolvent induced phase separation method显示文摘The micro-nano composite structure can endow separation membranes with special surface properties,but it often has the problems of inefficient preparation process and poor structural stability.In this work,a novel atomization-assisted nonsolvent induced phase separation method,which is also highly efficient and very simple,has been developed.By using this method,a bicontinuous porous microfiltration membrane with robust micro-nano composite structure was obtained via commercially available polymers of polyacrylonitrile and polyvinylpyrrolidone.The formation mechanism of the micro-nano composite structure was proposed.The microphase separation of polyacrylonitrile and polyvinylpyrrolidone components during the atomization pretreatment process and the hydrogen bonding between polyacrylonitrile and polyvinylpyrrolidone molecules should have resulted in the nano-protrusions on the membrane skeleton.The membrane exhibits superhydrophilicity in air and superoleophobicity underwater.The membrane can separate both surfactant-free and surfactant-stabilized oil-in-water emulsions with high separation efficiency and permeation flux.With excellent antifouling property and robust microstructure,the membrane can easily be recycled for long-term separation.Furthermore,the scale-up verification from laboratory preparation to continuous production has been achieved.The simple,efficient,cost-effective preparation method and excellent membrane properties indicate the great potential of the developed membranes in practical applications. | Jing Wang Guoyuan Pan Yu Li Yang Zhang Hongwei Shi Xuanbo Liu Hao Yu Muhua Zhao Yiqun Liu Changjiang Wu | 2022 | Frontiers of Chemical Science and Engineering2022，16，8： | 2 |
| 11 | Purification characteristics and parameters optimization of anthocyanin extracted from blueberry显示文摘To improve the yield and purity of anthocyanin extracted from blueberry,the characteristics and optimization for the separation and purification process of anthocyanin by AB-8 macroporous adsorbent resin was studied.In the absorption stage,the pH value has positive effect in ranges of 1.0-3.0 and the temperature rise has firstly negative then positive effect on adsorption rate of anthocyanin with adsorption time.In the desorption stage,the ethanol concentration increase has firstly positive then steady effect and the temperature rise has positive and then negative effect on desorption rate of anthocyanin.The purification temperature may improve diffusion or induce degradation of anthocyanin.AB-8 macroporous adsorbent resin is suitable to purify the anthocyanin from extract of powdered blueberries and the optimum parameters were obtained by using Design Expert software as adsorption time of 3 h,pH value of 1.0,adsorption temperature of 18℃ in absorption stage and desorption time of 30 min,ethanol concentration of 46.5%,desorption temperature of 22℃ in desorption stage with the highest absorption rate and desorption rate are 87.65% and 84.80%,respectively. | Zheng Xianzhe Zhang Zhaoguo Jin Changjiang Mu Yanqiu Liu Chenghai Chen Zhiying Liu Haijun Lin Zhen | 2015 | International Journal of Agricultural and Biological Engineering2015，8，2： | 2 |
| 12 | Mapping three-dimensional co-seismic surface deformations associated with the 2015 MW7.2 Murghab earthquake based on InSAR and characteristics of crustal strain显示文摘Three-dimensional(3 D) co-seismic surface deformations are of great importance to interpret the characteristics of coseismic deformations and to understand the geometries and dynamics of seismogenic faults. In this paper, we propose a method for mapping 3 D co-seismic deformations based on InSAR observations and crustal strain characteristics. In addition, the search strategy of correlation points is optimized by adaptive correlation distance, which greatly improves the applicability of the proposed method in restoring deformations in decorrelation areas. Results of the simulation experiment reveal that the proposed method is superior to conventional methods in both the accuracy and completeness. The proposed method is then applied to map the 3 D co-seismic surface deformations associated with the 2015 MW7.2 Murghab earthquake using ascending and descending ALOS-2 PALSAR-2 images. The results show that the seismogenic fault is the Sarez-Karakul fault(SKF), which is dominated by NE-SW strike slips with an almost vertical dip angle. The north section and the south segment near the epicentre have obvious subsidence along with a southwestward motion in the northwest wall, and the southeast wall has northeast movement and surface uplift trend along the fault zone. The strain field of the earthquake is also obtained by the proposed method. It is found that the crustal block of the seismic area is obviously affected by dilatation and shear forces, which is in good agreement with the movement character of the sinistral slip. | Jie GAN Jun HU Zhiwei LI Changjiang YANG Jihong LIU Qian SUN Wanji ZHENG | 2018 | Science China Earth Sciences2018，61，10： | 2 |
| 13 | Stability of the rarefaction wave for a two-fluid plasma model with diffusion显示文摘We study the large-time asymptotics of solutions toward the weak rarefaction wave of the quasineutral Euler system for a two-fluid plasma model in the presence of diffusions of velocity and temperature under small perturbations of initial data and also under an extra assumption θ_i,+/θ_e,+=θ_i,-/θ_e,-≥m_i/2m_e,namely, the ratio of the thermal speeds of ions and electrons at both far fields is not less than one half. Meanwhile,we obtain the global existence of solutions based on energy method. | DUAN RenJun LIU ShuangQian YIN HaiYan ZHU ChangJiang | 2016 | Science China Mathematics2016，59，1： | 2 |
| 14 | Edge superconductivity in multilayer WTe2 Josephson junction显示文摘WTe2,as a type-ⅡWeyl semimetal,has 2D Fermi arcs on the(O01)surface in the bulk and 1D helical edge states in its monolayer.These features have recently attracted wide attention in condensed matter physics.However,in the intermediate regime between the bulk and monolayer,the edge states have not been resolved owing to its closed band gap which makes the bulk states dominant.Here,we report the signatures of the edge superconductivity by superconducting quantum interference measurements in multilayerWTe2 Josephson junctions and we directly map the localized supercurrent.In thickWTe(~60nm),the supercurrent is uniformly distributed by bulk states with symmetricJosephson effect(|Ic+(B)|=|Ic^-(B)|).In thin WTe2(10 nm),however,the supercurrent becomes confined to the edge and its width reaches up to 1.4μm and exhibits non-symmetric behavior|Ic^+(B)|≠|Ic^-(B)|.The ability to tune the edge domination by changing thickness and the edge superconductivity establishes WTez as a promising topological system with exotic quantum phases and a rich physics. | Ce Huang Awadhesh Narayan Enze Zhang Xiaoyi Xie Linfeng Ai Shanshan Liu Changjiang Yi Youguo Shi Stefano Sanvito Faxian Xiu | 2020 | National Science Review2020，7，9： | 2 |
| 15 | A HIGH SENSITIVITY AND WIDE DYNAMIC RANGE ZERO-IF RF RECEIVER FOR COGNITIVE RADIO APPLICATION显示文摘This paper presents an RF receiver of zero-Intermediate Frequency(IF) architecture for Cognitive Radio(CR) communication systems.Zero-IF architecture reduce the image reject filter and IF filter,so it is excellent in low cost,compact volume,and low power dissipation.The receiver employs three digital attenuator and a high gain,high linearity low noise amplifier to achieve wide dynamic range of 70 dB and high receiving sensitivity of-81 dBm.A fully balanced I/Q demodulator and a differential Local Oscillator(LO) chips are used to minimize the negative effects caused by second-order distortion and LO leakage.In order to select an 8 MHz-channel from 14 continuous ones located in UHF band(694-806 MHz) accurately,approach of channel selectivity circuits is proposed.The RF receiver has been designed,fabricated,and test.The measured result shows that the noise figure is 3.4 dB,and the error vector magnitude is 7.5% when the input power is-81 dBm. | Liu Jing Zhu Xiaowei Zhang Xiaodong You Changjiang | 2010 | Journal of Electronics(China)2010，27，5： | 2 |
| 16 | Sperm cryopreservation in guppies and black mollies—A generalized freezing protocol for livebearers in Poeciliidae显示文摘 | Changjiang Huang Chunli Sun Xinyou Su Xingen Zhao Miao Miao Yueqin Liu Qiaoxiang Dong | 2009 | Cryobiology2009，，3： | 1 |
| 17 | Effects of the type of sintering atmosphere on the chro- mium leachability of thermal treated municipal solid waste incinerator fly ash 显示文摘 | WANG Kunsheng SUN Changjiang LIU Zhongyu | 2001 | Waste Management2001，21，1： | 1 |
| 18 | Ribosomal proteins S13 and L23 promote multidrug resistance in gastric cancer cells by suppressing drug-induced apoptosis显示文摘 | Yongquan Shi Huihong Zhai Xin Wang Zheyi Han Changjiang Liu Mei Lan Jingping Du Changcun Guo Yumei Zhang Kaichun Wu Daiming Fan | 2004 | Experimental Cell Research2004，，2： | 1 |
| 19 | Loss of the centrosomal protein Cenpj leads to dysfunction of the hypothalamus and obesity in mice显示文摘Cenpj is a centrosomal protein located at the centrosomes and the base of cilia,it plays essential roles in regulating neurogenesis and cerebral cortex development.Although centrosomal and cilium dysfunction are one of the causes of obesity,insulin resistance,and type 2 diabetes,the role that Cenpj plays in the regulation of body weight remains unclear.Here,we deleted Cenpj by crossing Cenpjflox/flox mice with Nkx2.1-Cre mice.Loss of the centrosomal protein Cenpj in Nkx2.1-expressing cells causes morbid obesity in mice at approximately 4 months of age with expended brain ventricles but no change of brain size.We found that hypothalamic cells exhibited reduced proliferation and increased apoptosis upon Cenpj depletion at the embryonic stages,resulting in a dramatic decrease in the number of Proopiomelanocortin(POMC)neurons and electrophysiological dysfunction of NPY neurons in the arcuate nucleus(ARC)in adults.Furthermore,depletion of Cenpj also reduced the neuronal projection from the ARC to the paraventricular nucleus(PVN),with decreased melanocortin-4 receptors(MC4R)expression in PVN neurons.The study defines the roles that Cenpj plays in regulating hypothalamus development and body weight,providing a foundation for further understanding of the pathological mechanisms of related diseases. | Wenyu Ding Changjiang Zhang Baisong Wang Xin Zhou Le Sun Suijuan Zhong Jing Liu Junjing Zhang Xiaoqun Wang Qian Wu | 2021 | Science China(Life Sciences)2021，64，3： | 1 |
| 20 | Homogeneous liquid–liquid extraction combined with gas chromatography–electron capture detector for the determination of three pesticide residues in soils显示文摘 | Xuedong Wang Xinna Zhao Xiujuan Liu Yanyan Li Lingyan Fu Jia Hu Changjiang Huang | 2008 | Analytica Chimica Acta2008，，1： | 1 |
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