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| 1 | Honeysuckle-encoded atypical microRNA2911 directly Largets influenza A viruses显示文摘 | Zhen Zhou Xihan Li Jinxiong Liu Lei Dong Qun Chen Jialing Liu Huihui Kongt Qianyi Zhang Xian Qi Dongxia Hou Lin Zhang Guoquan Zhang Yuchen Liu Yujing Zhang Jing Li Jin Wang Xi Chen Hua Wang Junfeng Zhang Hualan Chen Ke Zen Chen-Yu Zhang | 2015 | Cell Research2015，25，1： | 104 |
| 2 | aunalysis of the Genome Sequence of the Medicinal Plant Salvia miltiorrhiza显示文摘 | Haibin Xu Jingyuan Song Hongmei Luo Yujun Zhang Qiushi Li Yingjie Zhu Jiang Xu Ying Li Chi Song Bo Wang Wei Sun Guoan Shen Xin Zhang Jun Qian Aijia Ji Zhichao Xu Xiang Luo Liu He Chuyuan Li Chao Sun Haixia Yah Guanghong Cui Xiwen Li Xian 'en Li Jianhe Wei Juyan Liu Yitao Wang Alice Hayward David Nelson Zemin Ning Reuben J. Peters Xiaoquan Qi Shilin Chen | 2016 | Molecular Plant2016，9，6： | 70 |
| 3 | Mianlüe tectonic zone and Mianlüe suture zone on southern margin of Qinling-Dabie orogenic belt显示文摘The Mianle tectonic zone (Mianle zone), an ancient suture zone in addition to the Shangdan suture in the Qinling-Dabie orogenic belt, marks an important tectonic division geo-logically separating north from south and connecting east with west in China continent. To de-termine present structural geometry and kinematics in the Mianle tectonic zone and to recon-struct the formation and evolution history involving plate subduction and collision in the Qinling-Dabie orogenic belt, through a multidisciplinary study, are significant for exploring the mountain-building orogenesis of the central orogenic system and the entire process of the major Chinese continental amalgamation during the Indosinian. | ZHANG Guowei1, DONG Yunpeng1, LAI Shaocong1, GUO Anlin1, MENG Qingren2, LIU Shaofeng3, CHENG Shunyou1, YAO Anping1, ZHANG Zongqing4, PEI Xianzhi5 & LI Sanzhong6 1. Department of Geology, Northwest University, Xian 710069, China 2. Institute of Geology and Geophysics, Chinese Academy of Sciences, Beijing 100101, China 3. Earth Science and Resource Faculty, China University of Geosciences, Beijing 100083, China 4. Institute of Geology, Chinese Academy of Geological Sciences, Beijing 100037, China 5. Changan University, Xian 710054, China 6. Qingdao University of Oceanography, Qingdao 266003, China | 2004 | Science China Earth Sciences2004，47，4： | 57 |
| 4 | OsWRKY03, a rice transcriptional activator that functions in defense signaling pathway upstream of OsNPR1显示文摘WRKY family proteins are a class of plant specific transcription factors that involve in many stress response pathways. It has been shown that one Arabidopsis WRKY protein, AtWRKY29/22, is activated by MAP kinase signaling cascade and confers resistance to both bacterial and fungal pathogens. However, little is known about the biological roles of WRKY proteins in rice. In this study, we investigated the expression patterns of rice AtWRKY29/22 homolog, OsWRKY03, under different conditions, and also its possible role involved in plant defense. Our results showed that OsWRKY03 was up-regulated by several defense signaling molecules or different treatments. Further analysis revealed that the expres- sion of OsWRKY03 was light dependent. Transcriptional activation activity of OsWRKY03 was also demonstrated by yeast functional assay. Transient expression of OsWRKY03-GFP fusion protein in onion epidermis cells showed that OsWRKY03 was a nuclear localized protein. OsNPR1 as well as several other pathogenesis-related genes, such as OsPR1b, phenylalanine ammonia-lyase (ZB8) and peroxidase (POX22.3), were induced in OsWRKY03-overexpressing transgenic plants. These results indicated that OsWRKY03 is located upstream of OsNPR1 as a transcriptional activator in salicylic acid (SA)-dependent or jasmonic acid (JA)-dependent defense signaling cascades. | Xiao Qiang LIU Xian Quan BAI Qian QIAN Xiu Jie WANG Ming Sheng CHEN Cheng Cai CHU | 2005 | Cell Research2005，15，8： | 55 |
| 5 | Chinese Stroke Center Alliance:a national effort to improve healthcare quality for acute stroke and transient ischaemic attack:rationale,design and preliminary findings显示文摘Background In June 2015,the Chinese Stroke Association(CSA)initiated the Chinese Stroke Center Alliance(CSCA)to establish the national hospital-based stroke care quality assessment and improvement platform.This article outlines its objectives,operational structure,patient population,quality improvement(QI)intervention tools,data elements,data collection methodology and current patient and hospital data.Methods The CSCA is a national,hospital-based,multicentre,voluntary,multifaceted intervention and continuous QI initiative.This multifaceted intervention includes stroke centre development,written care protocols,workshops and a monitoring/feedback system of evidencebased performance measures.The data coordinating centre of the CSCA resides at the China National Clinical Research Center for Neurological Diseases,Beijing Tiantan Hospital.results As of July 2017,1576 hospitals in China have contributed detailed clinical information to serve as a benchmark for the stroke care quality of 433264 patients with acute stroke/transient ischaemic attacks(TIA),including 352572(81.38%)acute ischaemic stroke,30362(7.01%)TIA,42080(9.71%)spontaneous intracranial haemorrhage,5505(1.27%)subarachnoid haemorrhage and 2745(0.63%)not specified stroke.Conclusion The CSCA programme is designed to establish a continuous national stroke registry and help healthcare providers develop stroke centres and treat patients in a consistent manner in accordance with accepted national guidelines and,ultimately,improve patient outcomes.It supports the CSA mission to reduce stroke burden in China. | Yongjun Wang Zixiao Li Yilong Wang Xingquan Zhao Liping Liu Xin Yang Caiyun Wang Hongqiu Gu Fuying Zhang Chunjuan Wang Ying Xian David Z Wang Qiang Dong Anding Xu Jizong Zhao Chinese Stroke Center Alliance investigators | 2018 | Stroke & Vascular Neurology2018，3，4： | 38 |
| 6 | Compound Danshen injection improves endotoxin-induced microcirculatory disturbance in rat mesentery显示文摘AIM: To investigate the effect of compound Danshen injection on lipopolysaccharide (LPS)-induced rat mesenteric microcirculatory dysfunctions and the underlying possible mechanism by an inverted intravital microscope and high-speed video camera system. METHODS: LPS was continuously infused through the jugular artery of male Wistar rats at the dose of 2 mg/kg per hour. Changes in mesenteric microcirculation,such as diameters of arterioles and venules,velocity of RBCs in venules,leukocyte rolling,adhesion and emigration,free radicals released from post-capillary venules,FITC- albumin leakage and mast cell degranulation,were observed through an inverted intravital microscope assisted with CCD camera and SIT camera. Meanwhile,the expression of adhesion molecules CD11b/CD18 and the production of free radical in neutrophils,and the expression of intercellular adhesion molecule 1 (ICAM-1) in human umbilical vein endothelial cells (HUVECs) were quantified by flow cytometry (FACS) in vitro. RESULTS: The continuous infusion with LPS resulted in a number of responses in microcirculation,including a significant increase in the positive region of venulestained with Monastral blue B,rolling and adhesion of leukocytes,production of oxygen radical in venular wall,albumin efflux and enhanced mast cell degranulation in vivo,all of which,except for the leukocyte rolling,were attenuated by the treatment with compound Danshen injection. Experiments performed in vitro further revealed that the expression of CD11b/CD18 and the production of oxygen free radical in neutrophils,and the expression of ICAM-1 in HUVECs were increased by exposure to LPS,and they were attenuated by compound Danshen injection. CONCLUSION: These results suggest that compound Danshen injection is an efficient drug with multi-targeting potential for improving the microcirculatory disturbance. | Jing-Yan Han Yoshinori Horie Soichiro Miura, Yasutada Akiba Jun Guo Dan Li Jing-Yu Fan Yu-Ying Liu Bai-He Hu Li-Hua An Xin Chang Man Xu De-An Guo Kai Sun Ji-Ying Yang Shu-Ping Fang Ming-Ji Xian Masahiro Kizaki Hiroshi Nagata Toshifumi Hibi | 2007 | World Journal of Gastroenterology2007，13，26： | 26 |
| 7 | Transduction of Fas gene or Bcl-2 antisense RNA sensitizes cultured drug resistant gastric cancer cells to chemotherapeutic drugs显示文摘INTRODUCTIONChemotherapyisoneofthemajormethodsintumortreatment,butitoftendoesnotworkduetomultidrugresistance(MDR).Recentstudi... | XIAO Bing, SHI Yong Quan, ZHAO Yan Qiu, YOU Han, WANG Zuo You, LIU Xian Ling, YIN Fang, QIAO Tai Dong and FAN Dai Ming | 1998 | World Journal of Gastroenterology1998，4，5： | 24 |
| 8 | Report on Childhood Obesity in China (9): Sugar-sweetened Beverages Consumption and Obesity显示文摘Objective To explore the associations between sugar-sweetened beverage (SSB) consumption and obesity as well as obesity-related cardiometabolic disorders among children in China. Methods A total of 6974 (boys 3558, girls 3412) children aged 6-13 years participated in the study. Each participant's height, weight, waist circumference, fasting glucose, triglycerides, total cholesterol, high- density lipoprotein cholesterol, and low-density lipoprotein cholesterol were measured. The type of beverage consumption was determined using a self-administered questionnaire. Results SSBs were consumed regularly by 46.1% of the children. The prevalence [adjusted odds ratio (95% confidence internal (CI)] of obesity was 7.6% [as the reference group (ref.)], 10.1% [1.36(1.07, 1.74)], and 11.6% [1.46(1.21, 1.75)], among children who regularly drank milk, other beverages and SSBs, respectively. Regularly drinking SSBs elevated the likelihood of abdominal obesity [adjusted odds ratio (95% CI): 1.36 (1.17, 1.59)]. The prevalence [adjusted odds ratio (95% CI)] of obesity among children who regularly drank sports/caloric beverages, carbonated beverages, sweet tea, and plant protein beverages was 16.8% [2.00(1.31, 3.07)], 12.7% [1.52(1.23, 1.88)], 11.5% [1.52(1.18, 1.95)], and 10.4% [1.41(1.03, 1.94)], respectively, which was higher than that of regular milk drinkers [7.6 % (ref.)]. The prevalence [adjusted odds ratio (95% CI)] of abdominal obesity among children who regularly drank sweet tea, fruit/vegetable juices, and carbonated beverages was 17.7% [1.55(1.26, 1.90)], 16.2% [1.36(1.09, 1.70)], and 15.3% [1.24(1.03, 1.50)], respectively, which was much higher than that of regular milk drinkers [12.8% (ref.)]. Conclusions Regular SSB consumption was positively related to obesity and abdominal obesity. This relationship should be investigated further using a longitudinal study design. | SHANG Xian Wen LIU Ai Ling ZHANG Qian HU Xiao Qi DU Song Ming MA Jun XU Gui Fa LI Ying GUO Hong Wei DU Lin LI Ting Yu MA Guan Sheng | 2012 | Biomedical and Environmental Sciences2012，25，2： | 23 |
| 9 | Medusoid Fossils from the Sinian Xingmincun Formation of Southern Liaoning Province显示文摘Abundant medusoid soft-bodied metazoan fossils have been found in the Sinian Xingmincun Formation(with an isotopic age of 650 Ma) at Qipanmo, Qidingshan and Yangtun of the Dalian-Jinzhou area, southern Liaoning Province; in addition, a number of macroscopic algal fossils have been discovered for the first time in the Getun Formation overlying the Xingmincun Formation. Medusoid fossils include 3 genera and 6 species(all of which are new). Judging from their biological characters, these medusoid fossils are considered to have an intimate affinity with the Ediacara fauna in the terminal Precambrian of Australia, as their characters are close to each other. The authors suggest that these medusoids and the Edicara fauna might be the products of the same period. The discovery of the medusoid fauna in the Xingmincun Formation marks an important event in the evolution of the terminal Precambrian organisms in China, and it is of major practical significance in the study of the Sinian System of China and the correlation of the Sinian strata of northern and southern China. | Hong Zuomin, Huang Zhenfu, Yang Xinde, Lan Jing, Xian Bingcai, Yang Yajun, Regional Geological Survey Party, Bureau of Geology and Mineral Resources of Liaoning Province, Jinzhou, Liaoning and Liu Xiaoliang Shenyang Institute of Geology and Resources, Chinese Academy of Geological Sciences, Shenyang, Liaoning Fang Xinzhong | 1989 | Acta Geologica Sinica(English Edition)1989，63，1： | 20 |
| 10 | Wogonin protects glomerular podocytes by targeting Bcl-2-mediated autophagy and apoptosis in diabetic kidney disease显示文摘Diabetic kidney disease(DKD)is one of the microvascular complications of diabetes mellitus and a major cause of end-stage renal disease with limited treatment options.Wogonin is a flavonoid derived from the root of Scutellaria baicalensis Georgi,which has shown a potent renoprotective effect.But the mechanisms of action in DKD are not fully elucidated.In this study,we investigated the effects of wogonin on glomerular podocytes in DKD using mouse podocyte clone 5(MPC5)cells and diabetic mice model.MPC5 cells were treated with high glucose(30 mM).We showed that wogonin(4,8,16μM)dose-dependently alleviated high glucose(HG)-induced MPC5 cell damage,accompanied by increased expression of WT-1,nephrin,and podocin proteins,and decreased expression of TNF-α,MCP-1,IL-1βas well as phosphorylated p65.Furthermore,wogonin treatment significantly inhibited HG-induced apoptosis in MPC5 cells.Wogonin reversed HG-suppressed autophagy in MPC5 cells,evidenced by increased ATG7,LC3-II,and Beclin-1 protein,and decreased p62 protein.We demonstrated that wogonin directly bound to Bcl-2 in MPC5 cells.In HG-treated MPC5 cells,knockdown of Bcl-2 abolished the beneficial effects of wogonin,whereas overexpression of Bcl-2 mimicked the protective effects of wogonin.Interestingly,we found that the expression of Bcl-2 was significantly decreased in biopsy renal tissue of diabetic nephropathy patients.In vivo experiments were conducted in STZ-induced diabetic mice,which were administered wogonin(10,20,40 mg·kg^(−1)·d^(−1),i.g.)every other day for 12 weeks.We showed that wogonin administration significantly alleviated albuminuria,histopathological lesions,and p65 NF-κB-mediated renal inflammatory response.Wogonin administration dose-dependently inhibited podocyte apoptosis and promoted podocyte autophagy in STZ-induced diabetic mice.This study for the first time demonstrates a novel action of wogonin in mitigating glomerulopathy and podocytes injury by regulating Bcl-2-mediated crosstalk between autophagy and apoptosis.Wogonin may be a potential therapeutic drug against DKD. | Xue-qi Liu Ling Jiang Yuan-yuan Li Yue-bo Huang Xue-ru Hu Wei Zhu Xian Wang Yong-gui Wu Xiao-ming Meng Xiang-ming Qi | 2022 | Acta Pharmacologica Sinica2022，43，1： | 20 |
| 11 | Relative effects of phylogeny,biological characters and environments on leaf traits in shrub biomes across central Inner Mongolia,China显示文摘Aims Understanding the drivers for leaf traits is critical to improving our predictions on ecosystems'responses to global changes.Geographic patterns of leaf traits are shaped by phylogenetic,biological and environmental factors simultaneously.However,till now few studies have examined how these factors influenced leaf traits together,and how their effects differed at the within-and among-site levels.Methods We sampled leaf traits from a 1100 km shrub-biome transect across central Inner-Mongolia,including leaf mass per area(LMA),mass-based photosynthetic rate,nitrogen(N)and phosphorus(P)concentrations.We examined the effects of phylogenetic,biological(height and growth rate)and environmental(climate and soil)fac-tors on leaf traits with mixed-model analyses of variance.Variation partitioning method was used to separate the joint and independent effects of these three types of factors.Important Findings(i)Climate and soil fertility(total or available nutrient concentra-tions)together explained 11.4-41.4%of among-site variations,with remarkable difference among traits.(i)Height and height growth rate together explained 0.4--31.9%of trait variations(mostly among-site variations).Our results could only weakly support the ability of leaf traits as predictors for whole-plant growth.(ii)LMA was negatively related to height,which was consistent with the resource-use strategy hypothesis but incon-sistent with the hypotheses proposed for coexisting trees,suggest-ing that the LMA--height relationship is shaped by rather different mechanisms between the within-and among-communities lev-els.(iv)The variation partitioning analysis showed that,the rela-tionships between leaf traits and biological characters largely reflected the differences in both leaf traits and biological char-acters among species that occupying different sites.The relative importance of phylogenetic,biological and environmental fac-tors differed remarkably among leaf traits,between the within-and among-communities levels,and between different biomes.(v)Our results strongly suggest the necessity of examining the three types of factors simultaneously,and at both the within-and among-communities levels,for a better understanding of the drivers for leaf traits patterns. | Chao Liu Xiangping Wang Xian Wu Shuang Dai Jin-Sheng He Weilun Yin | 2013 | Journal of Plant Ecology2013，6，3： | 19 |
| 12 | Improved heterologous gene expression in Trichoderma reesei by cellobiohydrolase I gene (cbh1) promoter optimization显示文摘在 Trichoderma reesei 改进 heterologous 基因表达,一套最佳的人工的 cellobiohydrolase 我基因(cbh1 ) 倡导者被获得。到有三个潜在的葡萄糖抑压者结合部位的 -724 的从 -677 的区域被删除。包括 CCAAT 盒子和 Ace2 结合部位,然后,到修改 cbh1 倡导者的 -820 的从 -620 的区域反复被插入到修改 cbh1 倡导者,获得有拷贝数字的倡导者 2, 4,和 6。结果证明葡萄糖压抑效果被废除,当拷贝数字同时增加了,记者基因由这些多拷贝倡导者调整了的葡糖醛酸糖苷酶(gus ) 的表示水平显著地被提高。数据显示出使用倡导者的大诺言在细丝状的真菌增加 heterologous 基因表达的人工的修正策略并且为基因功能调查和紧张修正提供了一套可选的高表示的向量。 | Ti Liu Tianhong Wang Xian Li Xuan Liu | 2008 | Acta Biochimica et Biophysica Sinica2008，40，2： | 18 |
| 13 | Automated Counting of Rice Planthoppers in Paddy Fields Based on Image Processing显示文摘A quantitative survey of rice planthoppers in paddy fields is important to assess the population density and make forecasting decisions. Manual rice planthopper survey methods in paddy fields are time-consuming, fatiguing and tedious. This paper describes a handheld device for easily capturing planthopper images on rice stems and an automatic method for counting rice planthoppers based on image processing. The handheld device consists of a digital camera with WiFi, a smartphone and an extrendable pole. The surveyor can use the smartphone to control the camera, which is fixed on the front of the pole by WiFi, and to photograph planthoppers on rice stems. For the counting of planthoppers on rice stems, we adopt three layers of detection that involve the following:(a) the first layer of detection is an AdaBoost classifier based on Haar features;(b) the second layer of detection is a support vector machine(SVM) classifier based on histogram of oriented gradient(HOG) features;(c) the third layer of detection is the threshold judgment of the three features. We use this method to detect and count whiteback planthoppers(Sogatella furcifera) on rice plant images and achieve an 85.2% detection rate and a 9.6% false detection rate. The method is easy, rapid and accurate for the assessment of the population density of rice planthoppers in paddy fields. | YAO Qing XIAN Ding-xiang LIU Qing-jie YANG Bao-jun DIAO Guang-qiang TANG Jian | 2014 | Journal of Integrative Agriculture2014，13，8： | 18 |
| 14 | Increased serum levels of C-reactive protein and matrix metalloproteinase-9 in obstructive sleep apnea syndrome显示文摘背景妨碍的睡觉呼吸暂停症候群(OSAS ) ,由断断续续的 hypoxia/reoxygenation (IHR ) 描绘了,为心血管的疾病(CVD ) 作为一个独立风险因素被识别了。与 OSAS 联系的 CVD biomarkers 一直不是彻底地,有 OSAS 的 51 个人最近由 polysomnography 诊断了的 investigated.Methods 根据呼吸暂停的严厉被分类进二个组:对严重 OSAS 组中等(n= 28 ) 并且温和 OSAS 组(n= 23 ) 。可比较的年龄和身体团索引(BMI ) ,没有 OSAS, 25 个肥胖的人被选择为控制题目。浆液新陈代谢的变量, C 反应的蛋白质(CRP ) 和矩阵 metalloproteinase-9 (MMP-9 ) 被测量。枪兵关联和回归分析是 CRP 的 performed.Results 浆液集中, MMP-9 比在 25 个控制题目在 51 个 OSAS 病人是显著地更高的。CRP 和 MMP-9 的层次在病人与是显著地更高的与温和 OSAS 或在肥胖的控制题目比在病人节制到严重 OSAS。积极关联在 OSAS 病人在 CRP 和 MMP-9 的层次之间被发现。回归分析证明在好久调整和 BMI 以后,呼吸暂停 / 呼吸过慢过浅索引(AHI ) 显著地与 OSAS.Conclusions AHI 在病人与 CRP 和 MMP-9 的浆液集中相关,反射 IHR 的频率,是提高的传播 CVD biomarkers MMP-9 和 CRP 的一个预言者。我们的数据支持 IHR 贡献的理论在 OSAS 病人的煽动性的因素的 upregulation。 | YEJin LIU Hui LI Yuan LIU Xian ZHU Jie-ming | 2007 | Chinese Medical Journal2007，，17： | 17 |
| 15 | Effect of icarisid II on diabetic rats with erectile dysfunction and its potential mechanism via assessment of AGEs, autophagy, roTOR and the NO-cGMP pathway显示文摘可勃起的机能障碍(编辑) 是糖尿病 mellitus 的主要复杂并发症。Icariin 被显示了通过它的 bioactive 形式提高可勃起的功能, icarisid II。这研究经由对先进 glycosylation 结束的评价与编辑和它的潜在的机制在糖尿病的老鼠上调查 icarisid II 的效果产品(年龄) , autophagy, mTOR 和 NO-cGMP 小径。Icarisid II 被一个酶的方法从 icariin 提取。在控制和糖尿病的编辑组,老鼠被管理正常盐;在 icarisid II 组,老鼠 intragastrically 是管理 icarisid II。可勃起的功能被测量 intracavernosal 压力 / 平均数评估动脉的压力(ICP/MAP ) 。年龄集中,氮的氧化物 synthase (NOS ) 活动和 cGMP 集中被酶免疫分析估计。房间增长被分析使用甲基 thiazolyl tetrazolium 试金和流动 cytometry。Autophagosomes 被传播电子显微镜学, monodansylcadaverine 染色和 GFP-LC3 本地化观察。在 autophagy 的 NOS isoforms 和关键蛋白质的表示被西方的污点检验。我们的结果证明了 Icarisid II 增加了 ICP/MAP 价值,光滑的肌肉房间(SMC ) 生长曲线, S 阶段和 SMC/collagen 纤丝(SMC/CF ) 比例和减少的 Beclin 1 (P< 0.05 ) 。Icarisid II 显著地增加了 proliferative 索引和 p-p70S6K (Thr389 ) 层次并且减少 autophagosomes 的数字和 LC3-II 的层次(P< 0.01 ) 。Icarisid II 减少了年龄集中和增加的 cGMP 集中, NOS 活动(P< 0.05 ) 并且 cNOS 层次(P< 0.01 ) 在糖尿病的编辑组。因此, Icarisid II 组成有希望的混合物因为糖尿病的编辑和力量涉及 SMC 增长和 NO-cGMP 小径和年龄, autophagy 和 mTOR 小径的 downregulation 的 upregulation。 | Jian Zhang Ai-Min Li Bao-Xing Liu Fei Han Feng Liu Shao-Peng Sun Xin Li Shu-Jin Cui Shao-Zhong Xian Guang-Qi Kong Zhong-Cheng Xin Zhi-Li Ji | 2013 | Asian Journal of Andrology2013，15，1： | 17 |
| 16 | Clinical significance of Fas antigen expression in gastric carcinoma显示文摘INTRODUCTIONInordertodeterminewhetherFasantigenplaysaroleinthegastriccarcinogenicsequence,animmunohistochemicalstudyofFasant... | LIU Hai Feng 1, LIU Wei Wen 1, FANG Dian Chun 1, LIU Feng Xian 2 and HE Guang You 2 | 1999 | World Journal of Gastroenterology1999，5，1： | 15 |
| 17 | Electroacupuncture activates enteric glial cells and protects the gut barrier in hemorrhaged rats显示文摘AIM:To investigate whether electroacupuncture ST36 activates enteric glial cells,and alleviates gut inflammation and barrier dysfunction following hemorrhagic shock.METHODS:Sprague-Dawley rats were subjected to approximately 45% total blood loss and randomly divided into seven groups:(1) sham:cannulation,but no hemorrhage;(2) subjected to hemorrhagic shock(HS);(3) electroacupuncture(EA) ST36 after hemorrhage;(4) vagotomy(VGX)/EA:VGX before hemorrhage,then EA ST36;(5) VGX:VGX before hemorrhage;(6) a-bungarotoxin(BGT)/EA:intraperitoneal injection of a-BGT before hemorrhage,then EA ST36; and(7) a-BGT group:a-BGT injection before hemorrhage.Morphological changes in enteric glial cells(EGCs) were observed by immunofluorescence,and glial fibrillary acidic protein(GFAP; a protein marker of enteric glial activation) was evaluated using reverse transcriptase polymerase chain reaction and western blot analysis.Intestinal cytokine levels,gut permeability to 4-k Da fluorescein isothiocyanate(FITC)-dextran,and the expression and distribution of tight junction protein zona occludens(ZO)-1 were also determined.RESULTS:EGCs were distorted following hemorrhage and showed morphological abnormalities.EA ST36 attenuated the morphological changes in EGCs at 6 h,as compared with the VGX,a-BGT and HS groups.EA ST36 increased GFAP expression to a greater degree than in the other groups.EA ST36 decreased intestinal permeability to FITC-dextran(760.5 ± 96.43 ng/m L vs 2466.7 ± 131.60 ng/m L,P < 0.05) and preserved ZO-1 protein expression and localization at 6 h afterhemorrhage compared with the HS group.However,abdominal VGX and a-BGT treatment weakened or eliminated the effects of EA ST36.EA ST36 reduced tumor necrosis factor-a levels in intestinal homogenates after blood loss,while vagotomy or intraperitoneal injection of a-BGT before EA ST36 abolished its antiinflammatory effects.CONCLUSION:EA ST36 attenuates hemorrhageinduced intestinal inflammatory insult,and protects the intestinal barrier integrity,partly via activation of EGCs. | Sen Hu Zeng-Kai Zhao Rui Liu Hai-Bin Wang Chun-Yu Gu Hong-Min Luo Huan Wang Ming-Hua Du Yi Lv Xian Shi | 2015 | World Journal of Gastroenterology2015，21，5： | 15 |
| 18 | Immunotherapy using IL-2 and GM-CSF is a potential treatment for multidrug-resistant Mycobacterium tuberculosis显示文摘This study investigated the therapeutic effects of interleukin (IL)-2 and granulocyte-macrophage colony-stimulating factor (GM-CSF) coadministrated with antibacterial agents isoniazid (INH) and rifampin (RIF) to treat a mouse model of tuberculosis (TB) infection. A drug-susceptible TB strain, H37Rv was used to infect mice and the effectiveness of IL-2 and GM-CSF was initially evaluated based on survival rate, bacterial counts in lungs and spleens and the pathological condition of the lungs. Next, the therapeutic effect of the immunotherapy regimen was assessed in multidrug-resistant strain OB35-infected mice. In the H37Rv infection model, IL-2 and GM-CSF monotherapies reduced bacterial numbers in the lungs by 0.82 (P<0.01) and 0.58 (P<0.05) lg colony-forming units (CFU), respectively, and in the spleens by 1.42 (P<0.01) and 1.22 (P<0.01) lg CFU, respectively, compared with the untreated group. Mice receiving immunotherapy developed fewer lesions in the lungs compared with mice receiving antibacterial therapy alone. In the OB35 infection model, immunotherapy with either cytokine resulted in a significant reduction of bacterial load in the lungs and spleens and less severe lesions in the lungs compared with the untreated or antibacterial therapy treated mice. Notably, mice receiving immunotherapy with both cytokines had a 30% survival rate which was higher than that in other treated groups, and had significantly less CFUs in the lungs and spleens (1.02 and 1.34 lg CFU) compared with antibacterial therapy alone (P<0.01). This study demonstrated that immunotherapy with both IL-2 and GM-CSF may be useful to treat multidrug resistant tuberculosis (MDR-TB). | ZHANG YongRong LIU Jian WANG Yong XIAN Qiaoyang SHAO LingYun YANG Zhong WANG XiaoNing | 2012 | Science China(Life Sciences)2012，55，9： | 15 |
| 19 | Effects of warming and clipping on plant and soil properties of an alpine meadow in the Qinghai-Tibetan Plateau, China显示文摘Climate warming and livestock grazing are known to have great influences on alpine ecosystems like those of the Qinghai-Tibetan Plateau(QTP) in China. However, it is lacking of studies on the effects of warming and grazing on plant and soil properties in these alpine ecosystems. In this study, we reported the related research from manipulative experiment in 2010–2012 in the QTP. The aim of this study was to investigate the individual and combined effects of warming and clipping on plant and soil properties in the alpine meadow ecosystem. Infrared radiators were used to simulate climate warming starting in July 2010, while clipping was performed once in October 2011 to simulate the local livestock grazing. The experiment was designed as a randomized block consisting of five replications and four treatments: control(CK), warming(W), clipping(C) and warming+clipping combination(WC). The plant and soil properties were investigated in the growing season of the alpine meadow in 2012. The results showed that W and WC treatments significantly decreased relative humidity at 20-cm height above ground as well as significantly increases air temperature at the same height, surface temperature, and soil temperature at the depth of 0–30 cm. However, the C treatment did not significantly decrease soil moisture and soil temperature at the depth of 0–60 cm. Relative to CK, vegetation height and species number increased significantly in W and WC treatment, respectively, while vegetation aboveground biomass decreased significantly in C treatment in the early growing season. However, vegetation cover, species diversity, belowground biomass and soil properties at the depth of 0–30 cm did not differ significantly in W, C and WC treatments. Soil moisture increased at the depth of 40–100 cm in W and WC treatments, while belowground biomass, soil activated carbon, organic carbon and total nitrogen increased in the 30–50 cm soil layer in W, C and WC treatments. Although the initial responses of plant and soil properties to experimental warming and clipping were slow and weak, the drought induced by the downward shift of soil moisture in the upper soil layers may induce plant belowground biomass to transfer to the deeper soil layers. This movement would modify the distributions of soil activated carbon, organic carbon and total nitrogen. However, long-term data collection is needed to further explain this interesting phenomenon. | Man Hou XU Fei PENG Quan Gang YOU Jian GUO Xia Fei TIAN Min LIU Xian XUE | 2015 | Journal of Arid Land2015，7，2： | 14 |
| 20 | Determinants of Household Income Mobility in Rural China显示文摘这篇文章使用 multivariate 回归和分解分析评定家庭收入活动性决定因素和他们的贡献到在从 1989 ~ 2006 的农村中国的收入活动性。调查结果显示有更低的起始的收入的家庭铺平,工资收入,家庭成员的更高教育的水平,非农业的采用的家庭成员的更大的数字和更年轻的头的更高的份额是更活动的。而且除起始的收入以外,在工资收入的份额变化,在非农业的采用的家庭成员的份额变化,并且在家庭成员的教育的平均的年里变化是说明收入活动性的最重要的因素。这些调查结果在支持非农业的雇用和教育在农村中国提高家庭收入活动性的政策上要求更多的强调。 | Xuehua Shi Xiaoyun Liu Alexander Nuetah Xian Xin | 2010 | China & World Economy2010，18，2： | 14 |
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