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| 1 | Engineering Broad-Spectrum Bacterial Blight Resistance by Simultaneously Disrupting Variable TALE-Binding Elements of Multiple Susceptibility Genes in Rice显示文摘Xanthomonas oryzae pv.oryzae(Xoo),the causal agent of bacterial blight of rice,employs the transcription activator-like effectors(TALEs)to induce the expression of the OsSWEET family of putative sugar transporter genes,which function in conferring disease susceptibility(S)in rice plants.To engineer broadspectrum bacterial blight resistance,we used CRISPR/Cas9-mediated gene editing to disrupt the TALEbinding elements(EBEs)of two S genes,OsSWEETH and OsSWEET14,in rice cv.Kitaake,which harbors the recessive resistance allele of Xa25/OsSWEET13.The engineered rice line MS14K exhibited broadspectrum resistance to most Xoo strains with a few exceptions,suggesting that the compatible strains may contain new TALEs.We identified two PthXo2-like TALEs,Tal5LN18 and Tal7PX061,as major virulence factors in the compatible Xoo strains LN18 and PX061,respectively,and found that Xoo encodes at least five types of PthXo2-like effectors.Given that PthXo2/PthXo2.1 target OsSlVEETf3 for transcriptional activation,the genomes of 3000 rice varieties were analyzed for EBE variationsin the OsSWEET13 promoter,and 10Xa25-like haplotypes were identified.We found that Tal5LN18 and Tal7PX〇6i bind slightly different EBE sequences in the OsSWEET13 promoter to activate its expression.CRISPR/Cas9 technology was then used to generate InDels in the EBE of the OsSWEET13 promoter in MS14K to creat a new germplasm with three edited OsSWEET EBEs and broad-spectrum resistance against all Xoo strains tested.Collectively,our findings illustrate how to disarm TALE-S co-evolved loci to generate broad-spectrum resistance through the loss of effector-triggered susceptibility in plants. | Zhengyin Xu Xiameng Xu Qiang Gong Ziyang Li Ying Li Sai Wang Yangyang Yang Wenxiu Ma Longyu Liu Bo Zhu Lifang Zou Gongyou Chen | 2019 | Molecular Plant2019，12，11： | 29 |
| 2 | Urban Three-dimensional Expansion and Its Driving Forces——A Case Study of Shanghai,China显示文摘Urban expansion is a phenomenon of urban space increase,and an important measuring index of the process of urbanization.Taking Shanghai as an example,the changes of urban average height and built-up area were studied to represent city's vertical and horizontal increases respectively,and statistical methods were used to analyze the driving forces of urban expansion.The research drew following conclusions:1) The urban expansion process of Shanghai from 1985 to 2006 had a clear periodic feature,and could be divided into three stages:vertical expansion in dominance,coordinated vertical and horizontal expansion,and horizontal expansion in dominance.2) The average height and quantity of buildings in core city were significantly bigger than those in suburbs,but the changing speed of the latter was faster.And 3) urbanization process was the major driving force for the city's horizontal expansion,while industrial structure improvement was the key driving factor for the vertical expansion.Those two driving forces were simultaneously affected by city's political factors. | SHI Longyu SHAO Guofan CUI Shenghui LI Xuanqi LIN Tao YIN Kai ZHAO Jingzhu | 2009 | Chinese Geographical Science2009，19，4： | 20 |
| 3 | A Facile Recovery Process for Cathodes from Spent Lithium Iron Phosphate Batteries by Using Oxalic Acid显示文摘A simplistic and novel leaching process is developed to dispose spent lithium iron phosphate(LiFePO_(4))batteries.In this paper,oxalic acid is selected as a leaching reagent to recover lithium as a resource and remove phosphorus from LiFePO_(4) batteries,benefiting from its low natural effects.The physical properties of spent cathode materials(before leaching)and residues(after leaching)are identified by X-ray diffraction(XRD)and scanning electronic microscopy(SEM)with energy dispersive X-ray spectroscopy(EDS).The total amounts of Li and Fe are analyzed by inductively coupled plasma-atomic emission spectroscopy(ICP-AES).The process occurs under conditions of a 0.3 mol·L^(-1) oxalic acid concentration,a temperature of 80℃,a reaction time of 60 min and a solid/liquid ratio of 60 g·L^(-1).It can effectively precipitate92%ferrum in terms of FeC_(2)O_(4)·2H_(2)O from LiFePO4,with the leaching efficiency of Li achieving up to 98%.This method demonstrates a new strategy for dealing with spent lithium iron phosphate batteries with a low cost and low environmental impact. | Li Li Jun Lu Longyu Zhai Xiaoxiao Zhang Larry Curtiss Yi Jin Feng Wu Renjie Chen Khalil Amine | 2018 | CSEE Journal of Power and Energy Systems2018，4，2： | 12 |
| 4 | Whole genome re-sequencing reveals evolutionary patterns of sacred lotus (Nelumbo nucifera)显示文摘Sacred lotus(Nelumbo nucifera or lotus) is an important aquatic plant in horticulture and ecosystems. As a foundation for exploring genomic variation and evolution among different germplasms, we re-sequenced 19 individuals from three cultivated temperate lotus subgroups(rhizome,seed and flower lotus), one wild temperate lotus subgroup(wild lotus), one tropical lotus group(Thai lotus) and an outgroup(Nelumbo lutea). Through genetic diversity and polymorphism analysis by non-missing SNP sites widely distributed in the whole genome, we confirmed that wild and Thai lotus exhibited greater differentiation with a higher genomic diversity compared to cultivated lotus. Rhizome lotus had the lowest genomic diversity and a closer relationship to wild lotus, whereas the genomes of seed and flower lotus were admixed. Genes in energy metabolism process and plant immunity evolved rapidly in lotus, reflecting local adaptation.We established that candidate genes in genomic regions with significant differentiation associated with temperate and tropical lotus divergence always exhibited highly divergent expression pattern. Together, this study comprehensive and credible interpretates important patterns of genetic diversity and relationships, gene evolution, and genomic signature from ecotypic differentiation of sacred lotus. | Longyu Huang Mei Yang Ling Li Hui Li Dong Yang Tao Shi Pingfang Yang | 2018 | Journal of Integrative Plant Biology2018，60，1： | 8 |
| 5 | Photoreductive degradation of sulfur hexafluoride in the presence of styrene显示文摘硫 hexafluoride (SF6 ) 在空气作为最强大的温室气体之一被知道。由苯乙烯的 SF6 的减少的相片降级被学习了以达到为硫 hexafluoride 污染开发新奇补习。破坏和 SF6 的移动效率(DRE ) 上的反应条件的效果在这研究被检验。两起始的 styrene-to-SF6 比率和起始的氧集中对 DRE 施加重要影响。SF6 移动效率在 0.2 的起始的 styrene-to-SF6 比率到达最大的价值。DRE 与增加氧集中在 0 ~ 0.09 mol/m3 然后减少的范围上与氧集中增加,这被发现。当水蒸汽被喂进煤气的混合时, DRE 稍微在整个学习时间规模上被提高。X光检查光电子光谱学( XPS )分析,和煤气的层析团,光谱测定( GCMS )和 Fourier 转变红外线的光谱学(英尺红外)分析,证明那是将近居住在煤气的阶段的起始的氟的符号在 SiF4 形式的所有,而,起始的硫在元素的硫形式被扔,在相片降级以后。从有毒的副产品免费,相片降级可以为 SF6 消退面对苯乙烯用作一种有希望的技术。 | HUANG Li GU Dinghong Yang Longyu Xia Lanyan ZHANG Renxi HOU Huiqi | 2008 | Journal of Environmental Sciences2008，20，2： | 5 |
| 6 | The Xanthomonas typeⅢeffector Xop AP prevents stomatal closure by interfering with vacuolar acidification显示文摘Plant stomata close rapidly in response to a rise in the plant hormone abscisic acid(ABA)or salicylic acid(SA)and after recognition of pathogenassociated molecular patterns(PAMPs).Stomatal closure is the result of vacuolar convolution,ion efflux,and changes in turgor pressure in guard cells.Phytopathogenic bacteria secrete typeⅢeffectors(T3Es)that interfere with plant defense mechanisms,causing severe plant disease symptoms.Here,we show that the virulence and infection of Xanthomonas oryzae pv.oryzicola(Xoc),which is the causal agent of rice bacterial leaf streak disease,drastically increased in transgenic rice(Oryza sativa L.)plants overexpressing the Xoc T3E gene Xop AP,which encodes a protein annotated as a lipase.We discovered that Xop AP binds to phosphatidylinositol 3,5-bisphosphate(Ptd Ins(3,5)P_(2)),a memb rane phospholipid that functions in p H control in lysosomes,membrane dynamics,and protein trafficking.Xop AP inhibited the acidification of vacuoles by competing with vacuolar H^(+)-pyrophosphatase(V-PPase)for binding to Ptd Ins(3,5)P_(2),leading to stomatal opening.Transgenic rice overexpressing Xop AP also showed inhibition of stomatal closure when challenged by Xoc infection and treatment with the PAMP flg22.Moreover,Xop AP suppressed flg22-induced gene expression,reactive oxygen species burst and callose deposition in host plants,demonstrating that Xop AP subverts PAMP-triggered immunity during Xoc infection.Taken together,these findings demonstrate that Xop AP overcomes stomatal immunity in plants by binding to lipids. | Longyu Liu Ying Li Zhengyin Xu Huan Chen Jingyi Zhang Brittany Manion Fengquan Liu Lifang Zou Zheng Qing Fu Gongyou Chen | 2022 | Journal of Integrative Plant Biology2022，64，10： | 3 |
| 7 | Recent diagnostic developments at the 100 kJ-level laser facility in China显示文摘A 100 kJ-level laser facility has been designed to study inertial confinement fusion physics in China.This facility incorporates various diagnostic techniques,including optical,x-ray imaging,x-ray spectrum,and fusion product diagnostics,as well as general diagnostics assistance systems and central control and data acquisition systems.This paper describes recent developments in diagnostics at the facility. | Feng Wang Shaoen Jiang Yongkun Ding Shenye Liu Jiamin Yang Sanwei Li Tianxuan Huang Zhurong Cao Zhenghua Yang Xin Hu Wenyong Miao Jiyan Zhang Zhebin Wang Guohong Yang Rongqing Yi Qi Tang Longyu Kuang Zhichao Li Dong Yang Yulong Li Xiaoshi Peng Kuan Ren Baohan Zhang | 2020 | Matter and Radiation at Extremes2020，5，3： | 2 |
| 8 | ZAR1 resistosome and helper NLRs:Bringing in calcium and inducing cell death显示文摘To protect themselves from infectious diseases,plants have deployed a two-tiered surveillance system to recognize invading pathogens(Fu and Dong,2013).In the first layer of the immune system,plants evolved extracellular patter n recognition receptors to detect con served pathoge n-associated molecular patterns(PAM Ps)and activate PAMP-triggered immunity(PTI)(Boiler and Felix,2009). | Jian Chen Min Li Longyu Liu Gongyou Chen Zheng Qing Fu | 2021 | Molecular Plant2021，14，8： | 2 |
| 9 | Effect of Cryopreservation on Seed Viability of 4 Species显示文摘In order to explore the feasibility of cryopreservation on dormant forest tree seeds,the seed viability of 4 species(Malus baccata,Prunus davidiana,P.armenica and Amygdalu persica) with moisture content(MC) changing was tested after 30-day storage in liquid nitrogen of -196℃.The results showed that all the 4 species of seeds can keep a stable viability after cryopreservation.In the procedure,the optimum MC was 48 g/kg for M.baccata,27 g/kg for P.davidiana,31 g/kg for P.armenica and 45 g/kg for A.persica,... | LIU Yan~1 LI Qingmei~(2**) LIU Guangquan~(1**) DUAN Xinfang~3 HOU Longyu~1 1.International Research and Training Center on Erosion and Sedimentation,Beijing 100044,P.R.China 2.Research Institute of Forestry,Chinese Academy of Forestry,Beijing 100091,P.R.China 3.Institute of New Forestry Technology,Chinese Academy of Forestry,Beijing 100091,P.R.China | 2009 | Chinese Forestry Science and Technology2009，8，2： | 2 |
| 10 | Development and application of perfect SSR markers in cotton显示文摘Background:This study aimed to develop a set of perfect simple sequence repeat(SSR)markers with a single copy in the cotton genome,to construct a DNA fingerprint database suitable for authentication of cotton cultivars.We optimized the polymerase chain reaction(PCR)system for multi-platform compatibility and improving detection efficiency.Based on the reference genome of upland cotton and 10×resequencing data of 48 basic cotton germplasm lines,single-copy polymorphic SSR sites were identified and developed as diploidization SSR markers.The SSR markers were detected by denaturing polyacrylamide gel electrophoresis(PAGE)for initial screening,then fluorescence capillary electrophoresis for secondary screening.The final perfect SSR markers were evaluated and verified using 210 lines from different sources among Chinese cotton regional trials.Results:Using bioinformatics techniques,1246 SSR markers were designed from 26626 single-copy SSR loci.Adopting a stepwise(primary and secondary)screening strategy,a set of 60 perfect SSR markers was selected with high amplification efficiency and stability,easy interpretation of peak type,multiple allelic variations,high polymorphism information content(PIC)value,uniform chromosome distribution,and single-copy characteristics.A multiplex PCR system was established with ten SSR markers using capillary electrophoresis detection.Conclusions:A set of perfect SSR markers of cotton was developed and a high-throughput SSR marker detection system was established.This study lays a foundation for large-scale and standardized construction of a cotton DNA fingerprint database for authentication of cotton varieties. | WU Yuzhen HUANG Longyu ZHOU Dayun FU Xiaoqiong LI Chao WEI Shoujun PENG Jun KUANG Meng | 2020 | Journal of Cotton Research2020，3，3： | 2 |
| 11 | Biodiesel production from rice straw and restaurant waste employing black soldierfly assisted by microbes显示文摘 | Zheng Longyu Hou Yanfei Li Wu et a/ | 2012 | Energy2012，47，： | 1 |
| 12 | Preliminary investigation of the clinical significance of detecting circulating tumor cells en- riched from ling cancer patients显示文摘 | Chi Wu Huaijie Hao Longyue Li | 2009 | J Thorae Onco12009，4，： | 1 |
| 13 | Optimal index shooting policy for layered missile defense system显示文摘In order to cope with the increasing threat of the ballistic missile(BM)in a shorter reaction time,the shooting policy of the layered defense system needs to be optimized.The main decisionmaking problem of shooting optimization is how to choose the next BM which needs to be shot according to the previous engagements and results,thus maximizing the expected return of BMs killed or minimizing the cost of BMs penetration.Motivated by this,this study aims to determine an optimal shooting policy for a two-layer missile defense(TLMD)system.This paper considers a scenario in which the TLMD system wishes to shoot at a collection of BMs one at a time,and to maximize the return obtained from BMs killed before the system demise.To provide a policy analysis tool,this paper develops a general model for shooting decision-making,the shooting engagements can be described as a discounted reward Markov decision process.The index shooting policy is a strategy that can effectively balance the shooting returns and the risk that the defense mission fails,and the goal is to maximize the return obtained from BMs killed before the system demise.The numerical results show that the index policy is better than a range of competitors,especially the mean returns and the mean killing BM number. | LI Longyue FAN Chengli XING Qinghua XU Hailong ZHAO Huizhen | 2020 | Journal of Systems Engineering and Electronics2020，31，1： | 1 |
| 14 | Polymorphic amino acids at codons 9 and 37 of HIA-DQB1 alleles may confer susceptibility to cervical cancer among Chinese women 显示文摘 | Wu Yuping Chen Yulong Li Longyu | 2006 | Int J Cancer2006，118，： | 1 |
| 15 | Fabrication of hydroxyl group modified monodispersed hybrid silica particles band the h-SiO 2/TiO 2 core/shell microspheres as high performance photocatalyst for dye degradation显示文摘 | Shen Zheying Li Longyu Li Yat | | 0，，： | 1 |
| 16 | Ti2CTx MXene-based all-optical modulator显示文摘MXenes,a new class of 2D transition metal carbides,nitrides,and carbonitrides,have attracted much attention due to their outstanding properties.Here,we report the broadband spatial self-phase modulation of Ti2CTx MXene nanosheets dispersed in deionized water in the visible to near-infrared regime,highlighting the broadband nonlinear optical(NLO)response of Ti2CTx MXene.Using ultrafast pulsed laser excitation,the nonlinear refractive index n2 and the thirdorder nonlinear susceptibility χ^(3)monolayer of Ti2CTx MXene were measured to be^10^−13 m^2/W and ~10^−10 esu,respectively.Leveraging the large optical nonlinearity of Ti2CTx MXene,an all-optical modulator in the visible regime was fabricated based on the spatial cross-phase modulation effect.This work suggests that 2D MXenes are ideal broadband NLO materials with excellent prospects in NLO applications. | Jun Yi Jie Li Shuohan Huang Longyu Hu Lili Miao Chujun Zhao Shuangchun Wen Vadym N.Mochalin Apparao M.Rao | 2020 | InfoMat2020，2，3： | 1 |
| 17 | Exploring the potential of grease from yellow mealworm beetle ( Tenebrio molitor ) as a novel biodiesel feedstock显示文摘 | Longyu Zheng Yanfei Hou Wu Li Sen Yang Qing Li Ziniu Yu | 2013 | Applied Energy2013，，： | 1 |
| 18 | Seasonal distribution of increased precipitation in maternal environments influences offspring performance of Potentilla tanacetifolia in a temperate steppe ecosystem显示文摘Aims Precipitation is predicted to increase in arid and semiarid regions under climate change,with greater changes in intra-and interannual distribution in the future.As a major limiting factor in these regions,changes in precipitation undoubtedly influence plant growth and productivity.However,how the temporal shifts in pre-cipitation will impact plant populations are uncertain.Methods A 3-year field experiment and a greenhouse experiment were conducted in a temperate grassland in northern China to examine the impacts of seasonal(spring and summer)increased precipitation on offspring performance of a common species,Potentilla tanacetifolia.Important Findings Our results showed that the amounts and timing of increased precipitation both played important roles in regulating offspring performance of P.tanacetifolia in the temperate steppe ecosystem.Increased precipitation in spring at maternal stage stimulated seed production,germination percentage and seedling biomass,whereas increased precipitation in summer at maternal stage stimulated seedling biomass.The timing of increased precipitation influenced seed attributes,whereas the amount of increased precipitation influenced offspring seedling biomass.Our results indicate that population development of P.tanacetifolia may be underestimated under future increased precipitation regime,if the transgenerational effect is not taken into account. | Yang Li Longyu Hou Bing Song Shiqiang Wan Xiaoqin Sun Linghao Li | 2019 | Journal of Plant Ecology2019，12，4： | 1 |
| 19 | Synthesis of titania/polymer core-shell hybrid micropheres 显示文摘 | Li Longyu Qin Dianbin Yang Xinlin | 2010 | Colloid and Polymer Science2010，288，： | 1 |
| 20 | Biodiesel production from rice straw and restaurant waste employing black soldier fly assisted by microbes显示文摘 | Longyu Zheng Yanfei Hou Wu Li Sen Yang | 2012 | Energy2012，47，1： | 1 |
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