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| 1 | YTHDF2 is essential for spermatogenesis and fertility by mediating a wave of transcriptional transition in spermatogenic cells显示文摘The dynamic and reversible regulation roles of m^(6)A modification and the characterization of m^(6)A readers have provided new insights into spermatogenesis at the post-transcriptional level.YTHDF2,as an m^(6)A reader,has been reported to mediate the m^(6)A-containing transcript decay during the mouse oocyte maturation,embryonic stem cell differentiation,neural development,and zebrafish maternal-to-zygotic transition.However,the roles of YTHDF2 in mammalian spermatogenesis are uncertain.Here,we generated germ cell-specific Ythdf2 mutants(Ythdf2-vKO)at a C57BL/6J background and demonstrated that YTHDF2 is essential for mouse spermatogenesis and fertility.Ythdf2-vKO provides oligoasthenoteratozoospermia phenotype with increased apoptosis in germ cells.High-throughput RNA-seq analysis showed that a group of mRNAs is upregulated in Ythdf2-vKO mouse testis;further analysis and MeRIP-qPCR data showed that most of the upregulated genes in Ythdf2-vKO mouse testis are modified with m^(6)A and are YTHDF2 candidate binding genes.Interestingly,RNA-seq analysis combined with our previous single-cell transcriptomics data of mouse spermatogenesis pointed out the failure of a wave of transcript transition during the spermatogenesis of Ythdf2-vKO mice,which was confirmed by gene expression analysis using qPCR of diplotene spermatocytes and round spermatids obtained through fluorescence-activated cell sorting.Our study demonstrates the fundamental role of YTHDF2 during mouse spermatogenesis and provides a potential candidate for the diagnosis of male infertility with the oligoasthenoteratozoospermia syndrome. | Xinxi Zhao Zhen Lin Yong Fan Wenzhi Li Yujie Zhang Fei Li Tong Hong Hua Feng Minghan Tong Ningling Wang Yanping Kuang Qifeng Lyu | 2021 | Acta Biochimica et Biophysica Sinica2021，53，12： | 2 |
| 2 | Remediation performance and mechanisms of Cu and Cd contaminated water and soil using Mn/Al-layered double oxide-loaded biochar显示文摘The combined pollution of heavy metals is ubiquitous worldwide.Mn/Al-layered double oxide-loaded crab shells biochar (LDO/BC) was prepared,so as to remediate the combined pollution of Cd and Cu in soil and water.The pristine and used LDO/BC were characterized and the results revealed that the layered double oxide was successfully loaded on crab shells biochar (BC) and metal element Ca in crab shells was beneficial to the formation of more regular layered and flake structure.The maximal adsorption capacity (Qm) of LDO/BC for aqueous Cu^(2+)and Cd^(2+)was 66.23 and 73.47 mg/g,respectively.LDO/BC and BC were used to remediate e-waste-contaminated soil for the first time and exhibited highly efficient performance.The extraction amount of Cu and Cd in the contaminated soil by diethylene triamine penta-acetic acid (DTPA) after treating with 5% LDO/BC was significantly reduced from 819.84 to 205.95 mg/kg (with passivation rate 74.8%) and 8.46 to 4.16 mg/kg(with passivation rate 50.8%),respectively,inferring that the bioavailability of heavy metals declined remarkably.The experimental result also suggested that after remediation by LDO/BC the exchangeable and weak acid soluble Cu and Cd in soil translated to reducible,residual and oxidizable fraction which are more stable state.Precipitation,complexation and ion exchange were proposed as the possible mechanisms for Cd and Cu removal.In general,these experiment results indicate that LDO/BC can be a potentially effective reagent for remediation of heavy metal contaminated water and soil. | Pengcheng Lin Hang Liu Hua Yin Minghan Zhu Haoyu Luo Zhi Dang | 2023 | Journal of Environmental Sciences2023，，3： | 2 |
| 3 | Intrinsic kinetics of the alkylation of benzene with propylene over β zeolite catalyst显示文摘 | HAN Minghan LI Xiaojin LIN Shixiong | 2001 | Kinetics and Catalysis2001，42，4： | 1 |
| 4 | Numerical study of thermal instability in mixed convection flow over horizontal and inclined surfaces显示文摘 | Lin Minghan | 2002 | Int J Heat and Mass Transfer2002，45，1： | 1 |
| 5 | Ca^(2+)-dependent TaCCD1 cooperates with TaSAUR215 to enhance plasma membrane H^(+)-ATPase activity and alkali stress tolerance by inhibiting PP2C-mediated dephosphorylation of TaHA2 in wheat显示文摘Alkali stress is a major constraint for crop production in many regions of saline-alkali land.However,little is known about the mechanisms through which wheat responds to alkali stress.In this study,we identified a calcium ion-binding protein from wheat,TaCCD1,which is critical for regulating the plasma membrane(PM)H^(+)-ATPase-mediated alkali stress response.PM H+-ATPase activity is closely related to alkali tolerance in the wheat variety Shanrong 4(SR4).We found that two D-clade type 2C protein phosphatases,TaPP2C.D1 and TaPP2C.D8(TaPP2C.D1/8),negatively modulate alkali stress tolerance by dephosphorylating the penultimate threonine residue(Thr926)of TaHA2 and thereby inhibiting PM H+-ATPase activity.Alkali stress induces the expression of TaCCD1 in SR4,and TaCCD1 interacts with TaSAUR215,an early auxin-responsive protein.These responses are both dependent on calcium signaling triggered by alkali stress.TaCCD1 enhances the inhibitory effect of TaSAUR215 on TaPP2C.D1/8 activity,thereby promoting the activity of the PM H^(+)-ATPase TaHA2 and alkali stress tolerance in wheat.Functional and genetic analyses verified the effects of these genes in response to alkali stress,indicating that TaPP2C.D1/8 function downstream of TaSAUR215 and TaCCD1.Collectively,this study uncovers a new signaling pathway that regulates wheat responses to alkali stress,in which Ca^(2+)-dependent TaCCD1 cooperates with TaSAUR215 to enhance PM H+-ATPase activity and alkali stress tolerance by inhibiting TaPP2C.D1/8-mediated dephosphorylation of PM H+-ATPase TaHA2 in wheat. | Minghan Cui Yanping Li Jianhang Li Fengxiang Yin Xiangyu Chen Lumin Qin Lin Wei Guangmin Xia Shuwei Liu | 2023 | Molecular Plant2023，16，3： | 1 |
| 6 | Using DEA to evaluate R&D perfbrmance of the computers and peripherals firms in Taiwan 显示文摘 | Chen Chintai Chien Chenfu Lin Minghan | 2004 | International Journal of Business2004，9，34： | 1 |
| 7 | Influence of the gas distributor on the local hydrodynamic behavior of an external loop airlift reactor显示文摘 | Lin Jing Han Minghan Wang Tiefeng | 2004 | Chemical Engineering Journal2004，102，1： | 1 |
| 8 | Pressure drop for two phase counter current flow in a packed col- umn with a novel internal 显示文摘 | Han Minghan Lin Hongfei Yuan Yanhui | 2003 | Chemical Engineering Jour- nal2003，94，3： | 1 |
| 9 | A new panel containing specific spermatogenesis markers to identify spermatogenic cells in nonobstructive azoospermia patients显示文摘Infertility is a severe problem that affects 10%-15% of couples worldwide, among which male infertility is a factor in ~50% of the cases [1]. Azoospermia, which is defined as the absence of sperm in at least two ejaculates, can be divided into two types: obstructive azoospermia (OA) and non-OA (NOA)[1]. OA patients have normal spermatogenesis and mature sperm in testes and can father a biological child through testicular sperm retrieval and intracytoplasmic sperm injection (ICSI)[2]. By contrast, NOA patients usually have various degrees of spermatogenesis impairment and can be faced with complicated assisted reproductive technology (ART) strategies to father a biological child [2]. The precise diagnosis of spermatogenesis in NOA patients is crucial for clinical decision making regarding ART. | Xinxi Zhao Yiqin Wang Meng Ma Wei Zhang Jiqiang Si Wei Wang Zhen Lin Gang Han Charlie Degui Chen Minghan Tong Yanping Kuang Qifeng Lyu | 2019 | Acta Biochimica et Biophysica Sinica2019，51，6： | 1 |
| 10 | Therapeutic effect of Qinghuayin(清化饮)against chronic atrophic gastritis through the inhibition of toll or interleukin-1 receptor domain-containing adaptor inducing interferon-β signaling pathway显示文摘OBJECTIVE:To examine the efficacy of Qinghuayin(清化饮,QHY)in rat chronic atrophic gastritis(CAG)models and explored the molecular mechanism of QHY in treating CAG.METHODS:In total,65 Wistar rats were randomly divided into the control(n=10)and CAG groups(n=55).CAG model rats were further divided into five groups:model(n=10),vitacoenzyme(n=10),low-dose QHY(n=10),medium-dose QHY(n=10),and high-dose QHY groups(n=10).We analyzed histopathological changes using hematoxylin and eosin staining and measured interleukin(IL)-6 and IL-8 levels in serum using enzyme-linked immunosorbent assay(ELISA)(Boster Bio,Pleasanton,USA).In addition,gastrin(GAS),pepsinogen I(PGI),and PGII expressions were evaluated using ELISA.The protein and m RNA expression of toll-like receptor 4(TLR4)and toll or interleukin-1 receptor domaincontaining adaptor inducing interferon-β(TRIF)was detected by Western blotting and quantitative reverse transcription-polymerase chain reaction,respectively.RESULTS:Our results revealed that histopathological changes in CAG model rates could be restored by low-,medium-,and high-dose QHY.The changes in GAS and PGI/II expression demonstrated that QHY improved CAG.Serum IL-6 and IL-levels were decreased by QHY administration.TLR4 and TRIF were upregulated at the m RNA and protein levels in the model group but downregulated by QHY administration.CONCLUSION:We concluded that QHY could effectively improve the histopathological changes of the gastric mucosa induced by CAG in rats.The therapeutic mechanism of QHY may be related to inhibition of the inflammatory factors IL-6 and IL-8 and suppression of TLR4/TRIF m RNA and protein expression. | HE Youcheng YAO Xiaoling LI Sihan ZHOU Hongjian XU Ruoying ZHENG Rong XIAO Wendi SU Ning LIN Ping HUANG Minghan | 2022 | Journal of Traditional Chinese Medicine2022，42，2： | 1 |
| 11 | Pressure drop for two phase counter current flow in a packed col- umn with a novel internal显示文摘 | Han Minghan Lin Hongfei Yuan Yanhui | 2003 | Chemical Engineering Jour- nal2003，94，3： | 1 |
| 12 | Intrinsic kinetics of the alkylation of benzene with propylele over β zeolite catalyst 显示文摘 | HAN Minghan LI Xiaojin LIN Shixiong | 2001 | Kinetics and Catalysis2001，42，4： | 1 |
| 13 | Design of advanced porous silver powder with high-sintering activity to improve silicon solar cells显示文摘Silver(Ag)paste is widely used in semiconductor metallization,especially in silicon solar cells.Ag powder is the material with the highest proportion in Ag paste.The morphology and structure of Ag powder are crucial which determine its characteristics,especially for the sintering activity.In this work,a simple method was developed to synthesize a type of microcrystalline spherical Ag particles(SP-A)with internal pores and the structural changes and sintering behavior were thoroughly studied by combining ultra-small-angle X-ray scattering(USAXS),small-angle X-ray scattering(SAXS),in-situ heating X-ray diffraction(XRD),focused ion beam(FIB),and thermal analysis measurement.Due to the unique internal pores,the grain size of SP-A is smaller,and the coefficient of thermal expansion(CTE)is higher than that of traditional solid Ag particles.As a result,the sintering activity of SP-A is excellent,which can form a denser sintered body and form silver nanoparticles at the Ag–Si interface to improve silver silicon contact.Polycrystalline silicon solar cell built with SP-A obtained a low series resistance(Rs)and a high photoelectric conversion efficiency(PCE)of 19.26%.These fill a gap in Ag particle structure research,which is significant for the development of high-performance electronic Ag particles and efficient semiconductor devices. | Yongsheng Li Ziwei Chen Rui Zhou Wenguang Zhao Mu Li Jun Chen Zhongyuan Huang Jian Liu Yuhang Li Maolin Yang Minghan Yu Dong Zhou Yuan Lin Feng Pan | 2024 | Nano Research2024，17，4： | 0 |
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