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| 1 | Estimation of inbreeding and identification of regions under heavy selection based on runs of homozygosity in a Large White pig population显示文摘Background: Runs of homozygosity(ROHs) are homozygous segments of the genome where the two haplotypes inherited from the parents are identical. The current availability of genotypes for a very large number of single nucleotide polymorphisms(SNPs) is leading to more accurate characterization of ROHs in the whole genome. Here,we investigated the occurrence and distribution of ROHs in 3,692 Large White pigs and compared estimates of inbreeding coefficients calculated based on ROHs(FROH), homozygosity(FHOM), genomic relationship matrix(FGRM)and pedigree(FPED). Furthermore, we identified genomic regions with high ROH frequencies and annotated their candidate genes.Results: In total, 176,182 ROHs were identified from 3,569 animals, and all individuals displayed at least one ROH longer than 1 Mb. The ROHs identified were unevenly distributed on the autosomes. The highest and lowest coverages of Sus scrofa chromosomes(SSC) by ROH were on SSC14 and SSC13, respectively. The highest pairwise correlation among the different inbreeding coefficient estimates was 0.95 between FROH_totaland FHOM, while the lowest was-0.083 between FGRMand FPED. The correlations between FPEDand FROHusing four classes of ROH lengths ranged from 0.18 to 0.37 and increased with increasing ROH length, except for ROH > 10 Mb. Twelve ROH islands were located on four chromosomes(SSC1, 4, 6 and 14). These ROH islands harboured genes associated with reproduction, muscular development, fat deposition and adaptation, such as SIRT1, MYPN, SETDB1 and PSMD4.Conclusion: FROHcan be used to accurately assess individual inbreeding levels compared to other inbreeding coefficient estimators. In the absence of pedigree records, FROHcan provide an alternative to inbreeding estimates.Our findings can be used not only to effectively increase the response to selection by appropriately managing the rate of inbreeding and minimizing the negative effects of inbreeding depression but also to help detect genomic regions with an effect on traits under selection. | Liangyu Shi Ligang Wang Jiaxin Liu Tianyu Deng Hua Yan Longchao Zhang Xin Liu Hongmei Gao Xinhua Hou Lixian Wang Fuping Zhao | 2020 | Journal of Animal Science and Biotechnology2020，11，4： | 10 |
| 2 | Pitting corrosion of 2Cr13 stainless steel in deep-sea environment显示文摘Pitting corrosion of 2Cr13 stainless steel was investigated by deep-sea exposure test at various depths of 500 m,800 m and 1200 m in the South China Sea for 4 months.With the aid of electrochemical measurements in simulated deep-sea environments and grey relational analysis,the influence of deepsea environments on passive film and the mechanism of pitting corrosion were discussed.The results indicated that with the increase of sea depth,pitting depth of 2Cr13 stainless steel increased,which can be attributed to the change of chemical composition and the degradation of pitting resistance of passive film.Film growth was greatly retarded in the condition of low seawater temperature and low dissolved oxygen content of deep sea,resulting in an unstable and vulnerable film.Pitting depth was most influenced by hydrostatic pressure,which can increase the adsorption and penetration of Cl-ion,and promote the proliferation of point defects in passive film,leading to rapid deconstruction of protective oxides of the film.Pitting sensitivity of 2Cr13 stainless steel increased eventually with the combination of accelerated dissolution and suppressed self-healing of passive film in deep sea. | Xinhua Wang Lin Fan Kangkang Ding Likun Xu Weimin Guo Jian Hou Tigang Duan | 2021 | Journal of Materials Science & Technology2021，，5： | 6 |
| 3 | Genome-wide identification of RNA editing in seven porcine tissues by matched DNA and RNA high-throughput sequencing显示文摘Background: RNA editing is a co/posttranscriptional modification mechanism that increases the diversity of transcripts, with potential functional consequences. The advent of next-generation sequencing technologies has enabled the identification of RNA edits at unprecedented throughput and resolution. However, our knowledge of RNA editing in swine is still limited.Results: Here, we utilized RES-Scanner to identify RNA editing sites in the brain, subcutaneous fat, heart, liver,muscle, lung and ovary in three 180-day-old Large White gilts based on matched strand-specific RNA sequencing and whole-genome resequencing datasets. In total, we identified 74863 editing sites, and 92.1% of these sites caused adenosine-to-guanosine(A-to-G) conversion. Most A-to-G sites were located in noncoding regions and generally had low editing levels. In total, 151 A-to-G sites were detected in coding regions(CDS), including 94 sites that could lead to nonsynonymous amino acid changes. We provide further evidence supporting a previous observation that pig transcriptomes are highly editable at PRE-1 elements. The number of A-to-G editing sites ranged from 4155(muscle) to 25001(brain) across the seven tissues. The expression levels of the ADAR enzymes could explain some but not all of this variation across tissues. The functional analysis of the genes with tissuespecific editing sites in each tissue revealed that RNA editing might play important roles in tissue function.Specifically, more pathways showed significant enrichment in the fat and liver than in other tissues, while no pathway was enriched in the muscle.Conclusions: This study identified a total of 74863 nonredundant RNA editing sites in seven tissues and revealed the potential importance of RNA editing in tissue function. Our findings largely extend the porcine editome and enhance our understanding of RNA editing in swine. | Yuebo Zhang Longchao Zhang Jingwei Yue Xia Wei Ligang Wang Xin Liu Hongmei Gao Xinhua Hou Fuping Zhao Hua Yan Lixian Wang | 2019 | Journal of Animal Science and Biotechnology2019，10，2： | 4 |
| 4 | Allelic Analyses of the Arabidopsis YUC1 Locus Reveal Residues and Domains Essential for the Functions of YUC Family of Flavin Monooxygenases显示文摘Flavin monooxygenases(FMOs) play critical roles in plant growth and development by synthesizing auxin and other signaling molecules.However,the structure and function relationship within plant FMOs is not understood.Here we defined the important residues and domains of the Arabidopsis YUC1 FMO,a key enzyme in auxin biosynthesis.We previously showed that simultaneous inactivation of YUC1 and its homologue YUC4 caused severe defects in vascular and floral development.We mutagenized the yuc4 mutant and screened for mutants with phenotypes similar to those of yuc1 yuc4 double mutants.Among the isolated mutants,five of them contained mutations in the YUC1 gene.Interestingly,the mutations identified in the new yuc1 alleles were concentrated in the two GXGXXG motifs that are highly conserved among the plant FMOs.One such motif presumably binds to flavin adenine dinucleotide(FAD) cofactor and the other binds to nicotinamide adenine dinucleotide phosphate(NADPH).We also identified the Ser139 to Phe conversion in yuc1,a mutation that is located between the two nucleotide-binding sites.By analyzing a series of yuc1 mutants,we identified key residues and motifs essential for the functions of YUC1 FMO. | Xianhui Hou Sainan Liu Florencia Pierri Xinhua Dai Li-Jia Qu Yunde Zhao | 2011 | Journal of Integrative Plant Biology2011，53，1： | 4 |
| 5 | The landscape of chromatin accessibility in skeletal muscle during embryonic development in pigs显示文摘Background:The development of skeletal muscle in pigs during the embryonic stage is precisely regulated by transcriptional mechanisms,which depend on chromatin accessibility.However,how chromatin accessibility plays a regulatory role during embryonic skeletal muscle development in pigs has not been reported.To gain insight into the landscape of chromatin accessibility and the associated genome-wide transcriptome during embryonic muscle development,we performed ATAC-seq and RNA-seq analyses of skeletal muscle from pig embryos at 45,70 and 100 days post coitus(dpc).Results:In total,21,638,35,447 and 60,181 unique regions(or peaks)were found across the embryos at 45 dpc(LW45),70 dpc(LW70)and 100 dpc(LW100),respectively.More than 91%of the peaks were annotated within−1 kb to 100 bp of transcription start sites(TSSs).First,widespread increases in specific accessible chromatin regions(ACRs)from embryos at 45 to 100 dpc suggested that the regulatory mechanisms became increasingly complicated during embryonic development.Second,the findings from integrated ATAC-seq and RNA-seq analyses showed that not only the numbers but also the intensities of ACRs could control the expression of associated genes.Moreover,the motif screening of stage-specific ACRs revealed some transcription factors that regulate muscle developmentrelated genes,such as MyoG,Mef2c,and Mef2d.Several potential transcriptional repressors,including E2F6,OTX2 and CTCF,were identified among the genes that exhibited different regulation trends between the ATAC-seq and RNA-seq data.Conclusions:This work indicates that chromatin accessibility plays an important regulatory role in the embryonic muscle development of pigs and regulates the temporal and spatial expression patterns of key genes in muscle development by influencing the binding of transcription factors.Our results contribute to a better understanding of the regulatory dynamics of genes involved in pig embryonic skeletal muscle development. | Jingwei Yue Xinhua Hou Xin Liu Ligang Wang Hongmei Gao Fuping Zhao Lijun Shi Liangyu Shi Hua Yan Tianyu Deng Jianfei Gong Lixian Wang Longchao Zhang | 2021 | Journal of Animal Science and Biotechnology2021，12，4： | 2 |
| 6 | Effect of heavy metals on the stabilization of mercury(II) by DTCR in desulfurization solutions显示文摘 | Jiaai Hou Rongjie Lu Mingyang Sun Shams Ali Baig Tingmei Tang Lihua Cheng Xinhua Xu | 2012 | Journal of Hazardous Materials2012，，： | 1 |
| 7 | Stabilization of mercury in flue gas desulfurization gypsum from coal-fired electric power plants with additives显示文摘 | Mingyang Sun Jiaai Hou Tingmei Tang Rongjie Lu Lihua Cheng Xinhua Xu | 2012 | Fuel Processing Technology2012，，： | 1 |
| 8 | Multiple forms of vitamin B_(6) regulate salt tolerance by balancing ROS and abscisic acid levels in maize root显示文摘Salt stress causes osmotic stress,ion toxicity and oxidative stress,inducing the accumulation of abscisic acid(ABA)and excessive reactive oxygen species(ROS)production,which further damage cell structure and inhibit the development of roots in plants.Previous study showed that vitamin B_(6)(VB_(6))plays a role in plant responses to salt stress,however,the regulatory relationship between ROS,VB_(6) and ABA under salt stress remains unclear yet in plants.In our study,we found that salt stress-induced ABA accumulation requires ROS production,in addition,salt stress also promoted VB_(6)(including pyridoxamine(PM),pyridoxal(PL),pyridoxine(PN),and pyridoxal 5′-phosphate(PLP))accumulation,which involved in ROS scavenging and ABA biosynthesis.Furthermore,VB_(6)-deficient maize mutant small kernel2(smk2)heterozygous is more susceptible to salt stress,and which failed to scavenge excessive ROS effectively or induce ABA accumulation in maize root under salt stress,interestingly,which can be restored by exogenous PN and PLP,respec-tively.According to these results,we proposed that PN and PLP play an essential role in balancing ROS and ABA levels under salt stress,respectively,it laid a foundation for VB_(6) to be better applied in crop salt resistance than ABA. | Chongchong Lu Yuan Tian Xuanxuan Hou Xin Hou Zichang Jia Min Li Mingxia Hao Yanke Jiang Qingbin Wang Qiong Pu Ziyi Yin Yang Li Baoyou Liu Xiaojing Kang Guangyi Zhang Xinhua Ding Yinggao Liu | 2022 | Stress Biology2022，2，1： | 1 |
| 9 | Stabilizationof mercury in flue gas desulfurization gypsum fromcoal-fired electric power plants with additives 显示文摘 | Sun Mingyang Hou Jiaai Xu Xinhua | 2012 | FuelProcessing Technology2012，104，： | 1 |
| 10 | Sorption and ecotoxicity of pentachlorophenol polluted sediment amended with rice-straw derived biochar显示文摘 | Liping Lou Binbin Wu Lina Wang Ling Luo Xinhua Xu Jiaai Hou Bei Xun Baolan Hu Yingxu Chen | 2010 | Bioresource Technology2010，，5： | 1 |
| 11 | Revealing HOC1 burst from endoplasmic reticulum in cisplatin-treated cells via a ratiometric fluorescent probe显示文摘The reactive oxygen species(ROS) are tightly associated with endoplasmic reticulum(ER) stress.Thus,the deep and visual insight of aberrant ROS fluctuations in the ER can help us better investigate the ER stress-associated pathology.In this work,a fluorescent probe ERC for HOCI detection in the ER based on phenothiazine-derived coumarin platform was developed.In the presence of HOCI,ERC exhibited an emission change from 609 nm to 503 nm within seconds.It also showed high sensitivity(0.44 μmol/L)and superb photostability.Significantly,ERC displayed low cytotoxicity,good cell membrane permeability,and appreciable ER-targetability.Ultimately,the probe was successfully utilized to image exogenous and endogenous HOCl in living cells and reveal the HOCI burst in cisplatin-treated cancer cells. | Shan Wang Beitong Zhu Bingya Wang Xinhua Cao Lei Zhu Ji-Ting Hou Lintao Zeng | 2021 | Chinese Chemical Letters2021，32，5： | 1 |
| 12 | Performance improvement of n-i-p μc-Si:H solar cells by gradient hydrogen dilution technique显示文摘High pressure radio frequency plasma enhanced chemical vapor deposition(RF-PECVD)process was adopted to investigate the effect of constant hydrogen dilution technique and gradient hydrogen dilu-tion technique on the structural evolution of intrinsic films and the performance of n-i-p microcrystal-line silicon solar cells.The experiment results demonstrated that the grain size and crystalline volume fraction along the growth direction of intrinsic films can be controlled and the performance of solar cells can be greatly improved by gradient hydrogen dilution technique.An initial active-area efficiency of 5.7%(Voc=0.47V,Jsc=20.2mA/cm2,FF=60%)for the μc-Si:H single-junction n-i-p solar cells and an initial active-area efficiency of 10.12%(Voc=1.2V,Jsc=12.05mA/cm2,FF=70%)for the a-Si:H/μc-Si:H tandem n-i-p solar cells has been achieved. | YUAN YuJie HOU GuoFu ZHANG JianJun XUE JunMing CAO LiRan ZHAO Ying GENG XinHua | 2009 | Science China(Technological Sciences)2009，52，6： | 1 |
| 13 | Particle Sizes and Antibacterial Activity of Radix Astragalus and Radix Isatidis Ultrafine Powders显示文摘Using 9200 laser particle size analyzer and KYKY-2800 scanning electron microscope,particle sizes and cellular morphology of Radix Astragalus and Radix Isatidis ultrafine powders were observed. According to the results,the particle size of 89.1% of Radix Astragalus ultrafine powders ranged from 1.729 μm to 44. 938 μm,D_(50)=4.368 μm; t he particle size of 93.411% of Radix Isatidis ultrafine powders ranged from 1. 510 μm to 44. 938 μm,D50= 8.726 μm. Radix Astragalus and Radix Isatidis ultrafine powders were pulverized completely without intact cellular morphology. The antibacterial activity of Radix Astragalus and Radix Isatidis ultrafine powders against chicken-derived E. coli(O78) w as investigated. The results indicated that Radix Astragalus and Radix Isatidis ultrafine powders exhibited higher antibacterial activity against chicken-derived E. coli(O78) c ompared with the corresponding coarse powders. This study laid a solid foundation for the development and application of Chinese medicine ultrafine powder preparations. | Qiumei SHI Xinhua SHAO Xiumin WANG Xia MENG Xiaoqiao HOU Baoxin YANG Leiyu GUO Jinglong GAO | 2016 | Agricultural Biotechnology2016，5，4： | 1 |
| 14 | Sorption and ecotoxicity of pentachlorophenol polluted sediment amended with rice-straw derived biochar显示文摘 | Liping Lou Binbin Wu Lina Wang Ling Luo Xinhua Xu Jiaai Hou Bei Xun Baolan Hu Yingxu Chen | 2010 | Bioresource Technology2010，，5： | 1 |
| 15 | Design of hierarchical and mesoporous FeF_(3)/rGO hybrids as cathodes for superior lithium-ion batteries显示文摘Iron fluoride(FeF_(3)) is considered as a promising cathode material for Li-ion batteries(LIBs)due to its high theoretical capacity(712 mAh/g)with a 3 e-transfer.Herein,we have designed a strategy of hierarchical and mesoporous FeF_(3)/rG O hybrids for LIBs,where the hollow Fe F_(3) nanospheres are the main contributor to the specific capacity and the 2 D r GO nanosheets are the matrix elevating the electronic conductivity and buffering the volume expansion.The unique FeF_(3)/rGO hybrid can be rationally synthesized by a nonaqueous in-situ precipitation method,offering the merits of large specific surface area with rich active sites,fast transport channels for lithium ions,effective alleviation of volume expansion during cycles,and accelerating the electrochemical reaction kinetics.The Fe F_(3)/r GO hybrid electrode possesses a high initial discharge capacity of 553.9 m Ah/g at a rate of 0.5 C with 378 m Ah/g after 100 cycles,acceptable rate capability with 168 m Ah/g at 2 C,and feasible high-temperature operation(320 m Ah/g at 70℃).The superior electrochemical behaviors presented here demonstrates that the FeF_(3)/rGO hybrid is a potential electrode for LIBs,which may open up a new vision to design high-efficiency energy-storage devices such as LIBs based on transition metal fluorides. | Jiale Lian Yang Wu Yichuan Guo Zhenyun Zhao Qinghua Zhang Yang Hou Lingxiang Chen Bin Lu Xinhua Pan Zhizhen Ye Jianguo Lu | 2022 | Chinese Chemical Letters2022，33，8： | 0 |
| 16 | Associations of genome-wide structural variations with phenotypic differences in cross-bred Eurasian pigs显示文摘Background During approximately 10,000 years of domestication and selection,a large number of structural variations(SVs)have emerged in the genome of pig breeds,profoundly influencing their phenotypes and the ability to adapt to the local environment.SVs(≥50 bp)are widely distributed in the genome,mainly in the form of insertion(INS),mobile element insertion(MEI),deletion(DEL),duplication(DUP),inversion(INV),and translocation(TRA).While studies have investigated the SVs in pig genomes,genome-wide association studies(GWAS)-based on SVs have been rarely conducted.Results Here,we obtained a high-quality SV map containing 123,151 SVs from 15 Large White and 15 Min pigs through integrating the power of several SV tools,with 53.95%of the SVs being reported for the first time.These high-quality SVs were used to recover the population genetic structure,confirming the accuracy of genotyping.Potential functional SV loci were then identified based on positional effects and breed stratification.Finally,GWAS were performed for 36 traits by genotyping the screened potential causal loci in the F2 population according to their corresponding genomic positions.We identified a large number of loci involved in 8 carcass traits and 6 skeletal traits on chromosome 7,with FKBP5 containing the most significant SV locus for almost all traits.In addition,we found several significant loci in intramuscular fat,abdominal circumference,heart weight,and liver weight,etc.Conclusions We constructed a high-quality SV map using high-coverage sequencing data and then analyzed them by performing GWAS for 25 carcass traits,7 skeletal traits,and 4 meat quality traits to determine that SVs may affect body size between European and Chinese pig breeds. | Wencheng Zong Jinbu Wang Runze Zhao Naiqi Niu Yanfang Su Ziping Hu Xin Liu Xinhua Hou Ligang Wang Lixian Wang Longchao Zhang | 2024 | Journal of Animal Science and Biotechnology2024，15，1： | 0 |
| 17 | dBrms1 Acts as a Positive Regulator of Notch Signaling in Drosophila Wing显示文摘The highly conserved Notch signaling is precisely regulated at different steps in a series of developmental events. However, little is known about the regulation of Notch receptor at transcriptional level. Here, we demonstrate that dBrms1 is involved in regulating Notch signaling in Drosophila wing. We show that knockdown of dBrms1 by RNA interference(RNAi) in wing disc suppresses the expression of Notch signaling target genes wingless(wg), cut and Enhancer of split m8 [E(spl)m8]. Consistently, the levels of Wg and Cut are reduced in the dBrms1 mutant clones. Importantly, loss of dBrms1 leads to significant reduction of Notch proteins. Furthermore, depletion of dBrms1 results in apparent downregulation of Notch transcription in the wing disc. Moreover, we find that dBrms1 is functionally conserved with human Breast cancer metastasis suppressor 1 like(hBRMS1L) in the modulation of Notch signaling. Taken together, our data provide important insights into the biological function of dBrms1 in regulating Notch signaling. | Qinghai Zhang Yan Zhang Longfei Wu Yongfei Yang Xue Li Lei Gao Xiaomeng Hou Yihui Wu Guoli Hou Zhouhua Li Xinhua Lin | 2014 | Journal of Genetics and Genomics2014，41，6： | 0 |
| 18 | Multifunctional electroactive bio-adhesive for robustly-integrated wound therapy and postoperative wound-status warning and assessment显示文摘Wound abnormalities such as secondary wound laceration and inflammation are common postoperative health hazards during clinical procedures.The continuous treatment,healing induction,and real-time visualization of wound status and complications,including wound re-tearing,inflammation,and morphology,are key focal points for comprehensive healthcare.Herein,an on-demand quadruple energy dissipative strategy was proposed for the nanoengineering of a physically and chemically synergistic double-layer gelatin-based bio-adhesive(DLGel)by combining a multi-network adhesive layer and a versatile electroactive energy dissipative layer based on contrivable interlocking micro-pillar arrays and crosslinked polymer chains.The subtly multiple energy dissipation designs enable DLGel with robust adhesive strength to omnipotently wet and dynamic tissue,providing a basis for reliable wound closure.DLGel achieves comprehensive wound-healing induction through electrical stimulation and possesses reversible underwater light/thermal adhesion,excellent hemostatic performance,outstanding antimicrobial properties,and self-repair capability.Furthermore,a novel deep-learning strategy is creatively established to respond to mechanical deformation due to wound anomalies.This strategy translates biological information into visual graphics,providing real-time early warning and assessment of postoperative wound-abnormality/-morphology,such as laceration,inflammation,and necrosis.Therefore,DLGel and its associated signal collection and processing protocol enable the integration of reliable wound closure,wound healing,and real-time postoperative wound-status warning and assessment within the unobservable and undetectable“black box”regions in a context of non-clinical comprehensive therapy. | Ouyang Yue Xuechuan Wang Mengdi Hou Siwei Sun Manhui Zheng Xiaoliang Zou Zhongxue Bai Chunlin Liu Xinhua Liu | 2024 | Nano Research2024，17，5： | 0 |
| 19 | FOXK2激发广泛的转录抑制并阻遏缺氧反应和乳腺癌发生发展显示文摘临床上,严重智力障碍、West综合征、Dandy-Walker畸形和并指等临床表现与转录因子FOXK2功能障碍密切相关,然而目前FOXK2的具体生物学作用尚不明确。 | Lin Shan Xing Zhou Xinhua Liu Yue Wang Dongxue Su Yongqiang Hou Na Yu Chao Yang Beibei Liu Jie Gao Yang Duan Jianguo Yang Wanjin Li Jing Liang Luyang Sun Kexin Chen Chenghao Xuan Lei Shi Yan Wang 尚永丰 | 2017 | 科学新闻2017，19，4： | 0 |
| 20 | Seasonal Variability of Internal Tides Northeast of Taiwan显示文摘The spatial-temporal characteristics of the barotropic tides and internal tides(ITs) northeast of Taiwan Island are examined, based on a 1-year mooring current observations from May 23, 2017 to May 19, 2018. The results of harmonic tidal analysis show that the barotropic tides are dominated by semidiurnal tides, which is mainly controlled by M2 tidal components. Moreover, the vertical structures of diurnal and semidiurnal ITs show that the semidiurnal IT shows notable seasonal variation, whereas seasonal variations of the diurnal IT energy is not significant. The semidiurnal IT energy in winter half year is twice that in summer half year. The seasonal variation of semidiurnal IT is mainly modulated by the direction change of the current rather than by the topographic features and stratification. In summer(winter) half year cyclonic(anti-cyclonic) eddies meanly control at this point, so the flow direction is mainly in the southwest(northeast) direction, causing the background flow to flow along(perpendicular to) the isobath. When crossing the isobath, the ITs are generated by the interaction of the barotropic tide and the topography, resulting in the increase of the tidal energy in the winter half year. | ZHAO Xinhua HOU Yijun LIU Ze ZHUANG Zhanpeng WANG Kaidi | 2020 | Journal of Ocean University of China2020，19，3： | 0 |
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