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| 1 | Identification and bioinformatics analysis of lactate dehydrogenase genes from Echinococcus granulosus显示文摘Objective:To identify full length cDNA sequence of lactate dehydrogenase(LDH) from adult Echinococcus granulosus(E.granulosus) and to predict the structure and function of its encoding protein using bioinformatics methods.Methods:With the help of NCBI,EMBI, Expasy and other online sites,the open reading frame(ORF),conserved domain,physical and chemical parameters,signal peptide,epitope,topological structures of the protein sequences were predicted and a homology tertiary structure model was created:Vector NT1 software was used for sequence alignment,phylogenetic tree construction and tertiary structure prediction. Results:The target sequence was 1 233 bp length with a 996 bp biggest ORF encoding 331 amino acids protein with typical L-LDH conserved domain.It was confirmed as full length cDNA of LDH from E.granulosus and named as EgLDH(GenBank accession number:HM748917).The predicted molecular weight and isoelectric point of the deduced protein were 3 5516.2Da and 6.32 respectively.Compared with LDHs from Taenia solium,Taenia saginata asiatica,Spirometra erinaceieuropaei.Schistosoma japonicum,Clonorchis sinensis and human,it showed similarity of 86% ,85% ,55% ,58% ,58% and 53% ,respectively.EgLDH contained 3 putative transmembrane regions and 4 major epitopes(54aa-59aa.81aa-87aa,97aa-102aa,307aa-313aa),the latter were significant different from the corresponding regions of human LDH.In addition,some NAD and substrate binding sites located on epitopes 54aa-59aa and 97aa-102aa,respectively.Tertiary structure prediction showed that 3 key catalytic residues 105R,165D and 192H forming a catalytic center near the epitope 97aa-102aa,most NAD and substrate binding sites located around the center.Conclusions:The full length cDNA sequences of EgLDH were identified.It encoded a putative transmembrane protein which might be an ideal target molecule for vaccine and drugs. | Gang Lu Yajun Lu Lihua Li Lixian Wu Zhigang Fan Dazhong Shi Hu Wang Xiumin Han | 2010 | Asian Pacific Journal of Tropical Medicine2010，3，10： | 2 |
| 2 | Molecular identification of Echinococcus species from eastern and southern Qinghai, China, based on the mitochondrial cox1 gene显示文摘 | Junying Ma Hu Wang Gonghua Lin Philip S. Craig Akira Ito Zhenyuan Cai Tongzuo Zhang Xiumin Han Xiao Ma Jingxiao Zhang Yufang Liu Yanmei Zhao Yongshun Wang | 2012 | Parasitology Research2012，，1： | 2 |
| 3 | National Alveolar Echinococcosis Distribution--China, 2012−2016显示文摘What is already known about this topic?Both alveolar echinococcosis(AE)and cystic echinococcosis are endemic in China,among which alveolar echinococcosis has a very high mortality rate.What is added by this report?The survey results showed the prevalence and scope of AE in China and identified high-risk groups including children,monks,herdsmen and illiterate people.At the same time,all the cases found in the survey(more than 90%of the patients did not go to the hospital for diagnosis and treatment before survey)were promptly diagnosed and treated.What are the implications for public health practice?This study provides information for the development of a plan for AE prevention and control and for the implementation of interventions targeted to high-risk populations. | Canjun Zheng Chuizhao Xue Shuai Han Zhongjie Li Hu Wang Liying Wang Ying Wang Qian Wang Yu Feng Yanyan Hou Xiao Ma Junying Ma Xiumin Han Gongsang Quzhen Xiaofeng Jiang Weidong Guo Xianglin Wu Yaming Yang Lei Cao Fanka Li Weiqi Chen Xinliu Yan Shicheng Yu Meihua Fu Qing Yu Ning Xiao Jiangping Cao Jun Yan Weiping Wu Xiao-nong Zhou | 2020 | China CDC weekly2020，2，1： | 2 |
| 4 | Metarhizium taii var. chongqingensis Nov., Anamorph of Cordyceps chongqingensis sp. Nov. Isolated from a Low Altitude Area in Chongqing, China显示文摘 | Yanhong Yang Shaoxi Cai Yimin Zheng Xiumin Lu Xiuying Xu Yumei Han | 2009 | Current Microbiology2009，，6： | 1 |
| 5 | A TG-FTIR investigation to the catalytic effect of mineral matrix in oil shale on the pyrolysis and combustion of Kerogen显示文摘 | YAN Junwei JIANG Xiumin HAN Xiangxin | 2013 | Fuel2013，104，5： | 1 |
| 6 | Investigation of Chinese oilshale resources comprehensive utilization performance显示文摘 | Wang Sha Jiang Xiumin Han Xiangxin | 2012 | Energy2012，42，1： | 1 |
| 7 | Re- view of oil shale semicoke and its combustion utilization 显示文摘 | HAN Xiangxin KULAOTS I JIANG Xiumin | 2014 | Fuel2014，126，15： | 1 |
| 8 | In vitro protoscolicidal effects of high-intensity focused ultrasound enhanced by a superabsorbent polymer显示文摘 | Jing Zhang Bin Ye Jing Kong Hui Cai Yifeng Zhao Xiumin Han Faqi Li | 2013 | Parasitology Research2013，，1： | 1 |
| 9 | Devolatilization of oil sludge in a lab-scale bubbling fluidized bed显示文摘 | Jianguo Liu Xiumin Jiang Xiangxin Han | 2010 | Journal of Hazardous Materials2010，，2： | 1 |
| 10 | A TG-FTIR investigation to the catalytic effect of mineral matrix in oil shale on the pyrolysis and combustion of kerogen显示文摘 | Yan Junwei Jiang Xiumin Han Xiangxin Liu Jianguo | 2013 | Fuel2013，，104： | 1 |
| 11 | Characterization of Dachengzi Oil Shale Fast Pyrolysis By Curie-Point Py- rolysis-GC-MS显示文摘 | HUANG Yiru HAN Xiangxin JlANG Xiumin | 2015 | Oil Shale2015，32，2： | 1 |
| 12 | Investigation of Chinese oil shale resources comprehensive utilization performance显示文摘 | Sha Wang Xiumin Jiang Xiangxin Han | 2012 | Energy2012，，42： | 1 |
| 13 | Pyrolysis treatment of oil sludge and model-free kinetics analysis显示文摘 | Jianguo Liu Xiumin Jiang Lingsheng Zhou Xiangxin Han Zhigang Cui | 2008 | Journal of Hazardous Materials2008，，2： | 1 |
| 14 | Genetic polymorphisms of Echinococcus tapeworms in China as determined by mitochondrial and nuclear DNA sequences显示文摘 | Minoru Nakao Tiaoying Li Xiumin Han Xiumin Ma Ning Xiao Jiamin Qiu Hu Wang Tetsuya Yanagida Wulamu Mamuti Hao Wen Pedro L. Moro Patrick Giraudoux Philip S. Craig Akira Ito | 2009 | International Journal for Parasitology2009，，3： | 1 |
| 15 | Evaluation of structural characteristics of Huadian oil shale kerogen using direct techniques (solid-state 13C NMR, XPS, FT-IR, and XRD) 显示文摘 | Tong Jianhui Han Xiangxin Wang Sha Jiang Xiumin | 2011 | Energy & Fuels2011，25，： | 1 |
| 16 | Combustion characteristics of fine- and micro-pulverized coal in the mixture of 02/{202 显示文摘 | Huang Xiangyong Jiang Xiumin Han Xiangxin | 2008 | Energy and Fuels2008，22，6： | 1 |
| 17 | Deepening the Reform of College English Classroom Teaching-Exploration of Hierarchical Teaching in Class——Take Qingdao Huanghai University for Example显示文摘In the era of knowledge economy, the key is to cultivate talents with comprehensive quality. It’s known that people’s qualities are identical and different. Only by making full use of people’s differences, can we cultivate outstanding talents who conform to social development. Class hierarchical teaching as a new teaching mode, breaks the traditional class teaching mode, to a certain extent, in line with the requirements of quality education. Based on the college English situation of the students in the school where I’m teaching, this paper attempts to explore the hierarchical teaching of College English in class. | Xiumin Han | 2019 | Review of Educational Theory2019，2，2： | 0 |
| 18 | Fluid-Injection-Induced Seismicity Experiment of the WFSD-3P Borehole显示文摘In recent years,the fluid-injection-induced seismicity tests within the WFSD-3P borehole belonging to the Wenchuan Earthquake Fault Science Drilling(WFSD) project,have made remarkable progress.This study has been supported by the Science and Technology Project of China(No.WFSD-00003) | MA Xiumin LI Zhen PENG Hua JIANG Jingjie ZHAO Fang HAN Chaopu YUAN Pengxiang LU Shengzhou PENG Liguo | 2015 | Acta Geologica Sinica(English Edition)2015，89，3： | 0 |
| 19 | The clinical application and advancement of robot-assisted McKeown minimally invasive esophagectomy for esophageal cancer显示文摘Robotic surgery systems,as emerging minimally invasive approaches,have been increasingly applied for the treatment of esophageal cancer because they provide a high-definition three-dimensional surgical view and mechanical rotating arms that surpass the limitations of human hands,greatly enhancing the accuracy and flexibility of surgical methods.Robot-assisted McKeown esophagectomy(RAME),a common type of robotic esophagectomy,has been gradually implemented with the aim of reducing postoperative complications,improving postoperative recovery and achieving better long-term survival.Multiple centers worldwide have reported and summarized their experiences with the RAME,and some have also discussed and analyzed its perioperative effects and survival prognosis compared with those of video-assisted minimally invasive esophagectomy.Compared to traditional surgery,the RAME has significant advantages in terms of lymph node dissection although there seems to be no difference in overall survival or disease-free survival.With the continuous advancement of technology and the development of robotic technology,further development and innovation are expected in the RAME field.This review elaborates on the prospects of the application and advancement of the RAME to provide a useful reference for clinical practice. | Raojun Luo Yiming Li Xiumin Han Yunzheng Wang Zhengfu He Peijian Yan Ziyi Zhu | 2024 | Laparoscopic, Endoscopic and Robotic Surgery2024，7，1： | 0 |
| 20 | Changing Mortality and Patterns of Death Causes in HIV Infected Patients—China,2013-2022显示文摘What is already known about this topic?The advent of antiretroviral therapy(ART)has markedly decreased mortality rates among patients infected with human immunodeficiency virus(HIV).Globally,there has been a 43%reduction in acquired immunodeficiency syndrome(AIDS)-related deaths from 2010 to 2022.Additionally,prior research indicates that the initiation of ART at an early stage within China has substantially lowered mortality rates.What is added by this report?Over the previous decade,following the implementation of China’s universal ART access strategy,the patterns of mortality causes among HIVinfected individuals across the country have undergone significant alterations.In 2022,the all-cause mortality rate among this population was reported at 2.7%,with the non-AIDS-related mortality rate at 1.8%.However,it is important to consider that the accuracy of death reporting could contribute to potential misclassification of the underlying causes of death.What are the implications for public health practice?Efforts to enhance health outcomes should persist in emphasizing the advancement of ART strategies,with a particular focus on mitigating non-AIDS-related mortality in the future. | Yan Zhao Lai Wei Zhihui Dou Decai Zhao Xiumin Gan Yasong Wu Mengjie Han | 2023 | China CDC weekly2023，5，48： | 0 |
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