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| 1 | Prevalence of Autism Spectrum Disorder in China:A Nationwide Multi-center Population-based Study Among Children Aged 6 to 12 Years显示文摘This study aimed to obtain the first national estimate of the prevalence of autism spectrum disorder(ASD) in Chinese children.We targeted the population of 6 to 12-year-old children for this prevalence study by multistage convenient cluster sampling.The Modified Chinese Autism Spectrum Rating Scale was used for the screening process.Of the target population of 142,086 children,88.5%(n=125,806) participated in the study.A total of 363 children were confirmed as having ASD.The observed ASD prevalence rate was 0.29%(95% CI:0.26%-0.32%) for the overall population.After adjustment for response rates,the estimated number of ASD cases was867 in the target population sample,thereby achieving an estimated prevalence of 0.70%(95% CI:0.64%-0.74%).The prevalence was significantly higher in boys than in girls(0.95%;95% CI:0.87%-1.02% versus 0.30%;95%CI:0.26%-0.34%;P <0.001).Of the 363 confirmed ASD cases,43.3% were newly diagnosed,and most of those(90.4%) were attending regular schools,and 68.8% of the children with ASD had at least one neuropsychiatric comorbidity.Our findings provide reliable data on the estimated ASD prevalence and comorbidities in Chinese children. | Hao Zhou Xiu Xu Weili Yan Xiaobing Zou Lijie Wu Xuerong Luo Tingyu Li Yi Huang Hongyan Guan Xiang Chen Meng Mao Kun Xia Lan Zhang Erzhen Li Xiaoling Ge Lili Zhang Chunpei Li Xudong Zhang Yuanfeng Zhou Ding Ding Andy Shih Eric Fombonne Yi Zheng Jisheng Han Zhongsheng Sun Yong-hui Jiang Yi Wang LATENT-NHC Study Team | 2020 | Neuroscience Bulletin2020，36，9： | 158 |
| 2 | Meteorological mechanism for a large-scale persistent severe ozone pollution event over eastern China in 2017显示文摘An intensive and persistent regional ozone pollution event occurred over eastern China from 25 June to 5 July 2017.73 out of 96 selected cities,most located in the Beijing-TianjinHebei and the surrounding area(BTHS),suffered severe ozone pollution.A north-south contrast ozone distribution,with higher ozone(199±33μg/m3)in the BTHS and lower ozone(118±25μg/m^3)in the Yangtze River Delta(YRD),was found to be dominated by the position of the West Pacific Subtropical High(WPSH)and mid-high latitude wave activities.In the BTHS,the positive anomalies of geopotential height at 500 hPa and temperature at the surface indicated favorable meteorological conditions for local ozone formation.Prevailing northwesterly winds in the mid-high troposphere and warm advection induced by weak southerly winds in the low troposphere resulted in low-moderate relative humidity(RH),less total cloud cover(TGC),strong solar radiation and high temperatures.Moreover,southerly winds prevailing over the BTHS aggravated the pollution due to regional transportation of O3 and its precursors.On one hand,the deep sinking motion and inversion layer suppressed the dispersion of pollutants.On the other hand,O3-rich air in the upper layer was maintained at night due to temperature inversion,which facilitated O3 vertical transport to the surface in the next-day morning due to elevated convection.Generally,temperature,UV radiation,and RH showed good correlations with O3 in the BTHS,with rates of 8.51(μg/m^3)/℃(within the temperature range of 20-38℃),59.54(μg/m^3)/(MJ/m^2)and-1.93(μg/m^3)/%,respectively. | Jia Mao Lili Wang Chuhan Lu Jingda Liu Mingge Li Guiqian Tang Dongsheng Ji Nan Zhang Yuesi Wang | 2020 | Journal of Environmental Sciences2020，32，6： | 18 |
| 3 | Aluminium is essential for root growth and development of tea plants(Camellia sinensis)^∞显示文摘On acid soils,the trivalent aluminium ion(Al3+)predominates and is very rhizotoxic to most plant species.For some native plant species adapted to acid soils including tea(Camellia sinensis),Al3+has been regarded as a beneficial mineral element.In this study,we discovered that Al3+is actually essential for tea root growth and development in all the tested varieties.Aluminum ion promoted new root growth in five representative tea varieties with dose-dependent responses to Al3+availability.In the absence of Al3+,the tea plants failed to generate new roots,and the root tips were damaged within 1 d of Al deprivation.Struc-tural analysis of root tips demonstrated that Al was required for root meristem development and activity.In situ morin@staining of Al3+in roots revealed that Al mainly localized to nuclei in root meristem cells,but then gradually moved to the cytosol when Al3+was subsequently withdrawn.This movement of Al3+from nuclei to cytosols was accompanied by exacerbated DNA damage,which suggests that the nuclear-targeted Al primarily acts to maintain DNA integrity.Taken together,these results provide novel evidence that Al3+is essential for root growth in tea plants through maintenance of DNA integrity in meristematic cells. | Lili Sun Mengshi Zhang Xiaomei Liu Qianzhuo Mao Chen Shi Leon VKochian Hong Liao | 2020 | Journal of Integrative Plant Biology2020，62，7： | 12 |
| 4 | An unusual high ozone event over the North and Northeast China during the record-breaking summer in 2018显示文摘Under the background of global warming,the summer temperature of the North and Northeast China(NNEC)has significantly increased since 2017,which was accompanied by the aggravated ozone(O_(3))pollution.In 2018,the NNEC experienced a record-breaking summer of the past 40 years.Influenced by the abnormal high temperatures,a regional ozone event occurred on 2-3 August,over 63%of 79 selected cities in the NNEC were exposed to O_(3)pollution,and the maximum value of MDA8 O_(3)reached 268μg/m 3.Observations indicated that ozone concentrations agree well with the maximum temperature at 2 meters(MT2M)over NNEC with a correlation coefficient of 0.69.During the pollution episode,strong downdraft in the local high(35°N-42.5°N,112.5°E-132.5°E;LH)over the NNEC created the favourable meteorological conditions for O_(3)formation.By analyzing the horizontal wind and wave activity fluxes(WAFs)at 200 h Pa,we found that the LH formation was resulted from the Rossby wave propagation from upstream along the mid-latitude Asian jet.The split polar vortex intrusion further strengthened the amplitude of the Rossby wave and reinforced the LH.Moreover,a secondary circulation between Typhoon Jongdari and the LH contributed to the enhanced LH with strong subsidence.On the other hand,the stratospheric intrusions under the deep subsidence also contributed to the enhanced surface O_(3).In this study,the deep-seated meteorological dynamical mechanisms contributing to the abnormal high temperatures were investigated,which can lead to a better understanding of the regional O_(3)pollution over NNEC under the global-warming background. | Chuhan Lu Jia Mao Lili Wang Zhaoyong Guan Guangna Zhao Mingge Li | 2021 | Journal of Environmental Sciences2021，33，6： | 7 |
| 5 | Significance and expression of Bax, Survivin and p53 in gastric carcinoma and precancerous lesions using tissue microarray显示文摘Objective: To explore the relationship between expressions of apoptosis-related protein Bax, Survivin and p53 and the molecular mechanisms of carcinogenesis and progression of gastric carcinoma. Methods: Tissue microarray and immunohistochemistry were used in this study. Results: The positive rate of Bax protein in gastric cancer (17.7%,17/96) was significantly lower than those in adjacent normal mucosa (51%), intestinal metaplasia (69.2%) and dysplasia (75%), P < 0.01. The positive rate of Survivin expression in gastric cancer (80.6%, 89/98) was significantly higher than that in adjacent normal mucosa (3.9%), P < 0.01. The positive rates of Survivin expression in tumors with different organ metastases (in lymph node metastasis 86.2%, liver 100% and ovarian 100%) were statistically higher than in tumors without metastasis (64.3%), P < 0.05. Bax expression was correlated with Survivin but not with mp53 that was closely related to Survivin expression (P < 0.05) in gastric cancer. Conclusion: The abnormal expressions of Bax, Survivin and mp53 were correlated with the tumorigenesis and progression of gastric carcinoma. P53 and Survivin genes may share the similar mechanism in regulating cell apoptosis, and because of the mutation, p53 gene may lower its down-regulation to Survivin expression. | Yuping Xiao Zhi Lin Lili Mao Dongying Wu Yujia Gao Hongwei Sun Yan Xin | 2007 | The Chinese-German Journal of Clinical Oncology2007，6，4： | 5 |
| 6 | Integrated genomic analysis identifies deregulated JAK/STAT-MYC-biosynthesis axis in aggressive NK-cell leukemia显示文摘好攻击的 NK 房间白血病(ANKL ) 是在从亚洲的人之中是更流行的 NK 房间瘤的一种稀罕形式并且中央并且南美洲。病人们通常在天以内死到月,甚至在收到迅速的治疗学的管理以后。这里,我们由集成整个染色体执行了 ANKL 的第一全面研究, transcriptome 并且定序指向,象功能的试金一样的 cytokine 数组。在 JAK-STAT 小径的变化在 48% 被识别(14/29 ) ANKL 病人,当细胞外的 STAT3 激发器 IL10 被 56 褶层的一般水准提高时(P < 0.0001 ) 在检查的所有病人的血浆。另外的经常变异的基因包括的 TP53 (34%) , TET2 (28%) , CREBBP (21%) 和 MLL2 (21%) 。耐心的 NK 白血病房间在多重新陈代谢的小径显示出 STAT3 phosphorylation, MYC 表示和 transcriptional 活动的突出的激活。机能上地, STAT3 激活和 MYC 表示为 ANKL 房间的增长和幸存是批评的。发信号的 STAT 调整了发信号的 MYC 抄写节目,和两 STAT, MYC 抄写被要求维持核苷酸合成和 glycolysis 的激活。一起, JAK-STAT 小径在 ANKL 为 genomic 改变和 IL10 刺激代表一个主要目标。这最新发现的 JAK/STAT-MYC-biosynthesis 轴可以在对待白血病的这种子类型为新奇治疗学的策略的发展提供机会。 | Liang Huang Dan Liu Na Wang Shaoping Ling Yuting Tang Jun Wu Lingtong Hao Hui Luo Xuelian Hu Lingshuang Sheng Lijun Zhu Di Wang Yi Luo Zhen Shang Min Xiao Xia Mao Kuangguo Zhou Lihua Cao Lili Dong Xinchang Zheng Pinpin Sui Jianlin He Shanlan Mo Jin Yan Qilin Ao Lugui Qiu Hongsheng Zhou Qifa Liu Hongyu Zhang Jianyong Li Jie Jin Li Fu Weili Zhao Jieping Chen Xin Du Guoliang Qing Hudan Liu Xin Liu Gang Huang Ding Ma Jianfeng Zhou Qian-fei Wang | 2018 | Cell Research2018，28，2： | 5 |
| 7 | SNP-based QTL mapping for panicle traits in the japonica super rice cultivar Liaoxing 1显示文摘Panicle traits directly associated with yield are a target of selection in rice breeding.Although abundant QTL for panicle traits have been identified,there is little information about the genetic basis of panicle traits in japonica super rice(JSR)cultivars.In this study,we identified QTL for panicle traits in three environments using a population of recombinant inbred lines(RILs)derived from the JSR cultivar Liaoxing 1.A total of 197 RILs were genotyped with 285 polymorphic SNP markers.Phenotypic data and best linear unbiased prediction(BLUP)value of primary branch number(BNP),secondary branch number(BNS),grain number on primary branch(GNP),grain number on secondary branch(GNS),grain number per panicle(GN),panicle length(PL)and grain density(GD)were used for QTL mapping.A total of 105 QTL for seven panicle traits were detected in single environments using their BLUP values.Individual QTL explained 0.51%–52.22%of the phenotypic variation.Of the 105,49 were also detected by joint multi-environment analyses.Five stable QTL:qGD9,qPL9,qGNP9,qGN6,and qBNS6.2 were identified in multiple environments.qGD9,qGNP9,and qPL9,co-localizing on chromosome 9,likely correspond to the known gene DEP1.Importantly,qGN6 and qBNS6.2 in a co-localization region were identified as novel QTL,and their Liaoxing 1 alleles had a positive effect.Several RILs with the QTL allele combinations qGD9/qPL9/qGNP9 and qGN6/qBNS6.2 showed greater GN.Further investigation of the putative gene underlying qGN6/qBNS6.2 would shed light on the molecular mechanism of JSR. | Mingzhu Zhao Zuobin Ma Lili Wang Zhiqiang Tang Ting Mao Chuanbin Liang Hong Gao Liying Zhang Na He Liang Fu Changhua Wang Guomin Sui Wenjing Zheng | 2020 | The Crop Journal2020，8，5： | 4 |
| 8 | Charge‑Transfer Resonance and Electromagnetic Enhancement Synergistically Enabling MXenes with Excellent SERS Sensitivity for SARS‑CoV‑2 S Protein Detection显示文摘The outbreak of coronavirus disease 2019 has seriously threatened human health.Rapidly and sensitively detecting SARSCoV-2 viruses can help control the spread of viruses.However,it is an arduous challenge to apply semiconductor-based substrates for virus SERS detection due to their poor sensitivity.Therefore,it is worthwhile to search novel semiconductor-based substrates with excellent SERS sensitivity.Herein we report,for the first time,Nb2C and Ta2C MXenes exhibit a remarkable SERS enhancement,which is synergistically enabled by the charge transfer resonance enhancement and electromagnetic enhancement.Their SERS sensitivity is optimized to 3.0×10^6 and 1.4×10^6 under the optimal resonance excitation wavelength of 532 nm.Additionally,remarkable SERS sensitivity endows Ta2C MXenes with capability to sensitively detect and accurately identify the SARS-CoV-2 spike protein.Moreover,its detection limit is as low as 5×10^−9 M,which is beneficial to achieve real-time monitoring and early warning of novel coronavirus.This research not only provides helpful theoretical guidance for exploring other novel SERS-active semiconductor-based materials but also provides a potential candidate for the practical applications of SERS technology. | Yusi Peng Chenglong Lin Li Long Tanemura Masaki Mao Tang Lili Yang Jianjun Liu Zhengren Huang Zhiyuan Li Xiaoying Luo John RLombardi Yong Yang | 2021 | Nano-Micro Letters2021，13，3： | 3 |
| 9 | miR-20a Promotes the Axon Regeneration of DRG Neurons by Targeting Nr4a3显示文摘Dear Editor,The peripheral nervous system(PNS)regenerates more easily after injury than the central nervous system(CNS)[1].Sensory neurons in the L4–L6 dorsal root ganglia(DRGs)extend axons to form the sciatic nerve along with motor axons.The DRG neuron is one of the exceptional mature neurons whose axons can regenerate after injury. | Lili Zhao Leilei Gong Ping Li Jing Qin Lingchi Xu Qiyao Wei Huimin Xie Susu Mao Bin Yu Xiaosong Gu Songlin Zhou | 2021 | Neuroscience Bulletin2021，37，4： | 3 |
| 10 | Rapid, automated, and reliable antimicrobial susceptibility test from positive blood culture by CAST‐R显示文摘Antimicrobial susceptibility tests(ASTs)are pivotal in combating multidrug resistant pathogens,yet they can be time‐consuming,labor‐intensive,and unstable.Using the AST of tigecycline for sepsis as the main model,here we establish an automated system of Clinical Antimicrobials Susceptibility Test Ramanometry(CAST‐R),based on D2O‐probed Raman microspectroscopy.Featuring a liquid robot for sample pretreatment and a machine learning‐based control scheme for data acquisition and quality control,the 3‐h,automated CAST‐R process accelerates AST by>10‐fold,processes 96 paralleled antibiotic‐exposure reactions,and produces high‐quality Raman spectra.The Expedited Minimal Inhibitory Concentration via Metabolic Activity is proposed as a quantitative and broadly applicable parameter for metabolism‐based AST,which shows 99%essential agreement and 93%categorical agreement with the broth microdilution method(BMD)when tested on 100 Acinetobacter baumannii isolates.Further tests on 26 clinically positive blood samples for eight antimicrobials,including tigecycline,meropenem,ceftazidime,ampicillin/sulbactam,oxacillin,clindamycin,vancomycin,and levofloxacin reveal 93%categorical agreement with BMD‐based results.The automation,speed,reliability,and general applicability of CAST‐R suggest its potential utility for guiding the clinical administration of antimicrobials. | Pengfei Zhu Lihui Ren Ying Zhu Jing Dai Huijie Liu Yuli Mao Yuandong Li Yuehui He Xiaoshan Zheng Rongze Chen Xiaoting Fu Lili Zhang Lijun Sun Yuanqi Zhu Yuetong Ji Bo Ma Yingchun Xu Jian Xu Qiwen Yang | 2022 | mLife2022，1，3： | 3 |
| 11 | The Functional Characterization of Bat and Human P[3] Rotavirus VP8*s显示文摘P[3]rotavirus(RV)has been identified in many species,including human,simian,dog,and bat.Several glycans,including sialic acid,histo-blood group antigens(HBGAs)are reported as RV attachment factors.The glycan binding specificity of different P[3]RV VP8*s were investigated in this study.Human HCR3 A and dog P[3]RV VP8*s recognized glycans with terminal sialic acid and hemagglutinated the red blood cells,while bat P[3]VP8*showed neither binding to glycans nor hemagglutination.However,the bat P[3]VP8*mutant of C189 Y obtained the ability to hemagglutinate the red blood cells,while human P[3]HCR3 A/M2-102 mutants of Y189 C lost the ability.Sequence alignment and structural analysis indicated that residue 189 played an important role in the ligand recognition and may contribute to the cross-species transmission.Structural superimposition exhibited that bat P[3]VP8*model was quite different from the simian P[3]Rhesus rotavirus(RRV)P[3]VP8*,indicating that bat P[3]RV was relatively distinct and partially contributed to the no binding to tested glycans.These results promote our understanding of P[3]VP8*/glycans interactions and the potential transmission of bat/human P[3]RVs,offering more insight into the RV infection and prevalence. | Dandi Li Mengxuan Wang Tongyao Mao Mingwen Wang Qing Zhang Hong Wang Lili Pang Xiaoman Sun Zhaojun Duan | 2021 | Virologica Sinica2021，36，5： | 3 |
| 12 | Methods for measuring soil infiltration:State of the art显示文摘Soil infiltration is the process by which water on the soil surface penetrates the soil.Quantifying the soil infiltration capacity(soil infiltrability)is very important for determining components of the hydrological modeling,irrigation design and many other natural or man made processes.In this paper,commonly used methods for soil infiltration rate measurement with their principles and application conditions are introduced.The advantages and disadvantages of each method under various application conditions are discussed for comparison.Three new methods for soil infiltrability measurement,including the corresponding algorithm models,and the experimental apparatus and procedures are introduced.These analyses should facilitate the choice of method used for soil infiltrability measurement. | Mao Lili V F Bralts Pan Yinghua Liu Han Lei Tingwu | 2008 | International Journal of Agricultural and Biological Engineering2008，1，1： | 2 |
| 13 | Chemotherapy initiation with single-course methotrexate alone or combined with dactinomycin versus multi-course methotrexate for low-risk gestational trophoblastic neoplasia: a multi-centric randomized clinical trial显示文摘We aimed to evaluate the effectiveness and safety of single-course initial regimens in patients with low-risk gestational trophoblastic neoplasia(GTN).In this trial (NCT01823315),276 patients were analyzed.Patients were allocated to three initiated regimens:single-course methotrexate(MTX),single-course MTX+dactinomycin(ACTD),and multi-course MTX(control arm).The primary endpoint was the complete remission(CR)rate by initial drug(s).The primary CR rate was 64.4%with multi-course MTX in the control arm.For the single-course MTX arm,the CR rate was 35.8%by one course;it increased to 59.3%after subsequent multi-course MTX,with non-inferiority to the control(difference-5.1%,95%confidence interval(CI)-19.4%to 9.2%,P=0.014).After further treatment with multi-course ACTD,the CR rate(93.3%)was similar to that of the control(95.2%,P=0.577).For the single-course MTX+ACTD arm,the CR rate was 46.7%by one course,which increased to 89.1%after subsequent multi-course,with non-inferiority(difference 24.7%,95%CI 12.8%-36.6%,P<0.001)to the control.It was similar to the CR rate by MTX and further ACTD in the control arm(89.1%vs.95.2%,P=0.135).Four patients experienced recurrence,with no death,during the 2-year follow-up.We demonstrated that chemotherapy initiation with single-course MTX may be an alternative regimen for patients with low-risk GTN. | Lili Chen Ling Xi Jie Jiang Rutie Yin Pengpeng Qu Xiuqin Li Xiaoyun Wan Yaxia Chen Dongxiao Hu Yuyan Mao Zimin Pan Xiaodong Cheng Xinyu Wang Qingli Li Danhui Weng Xi Zhang Hong Zhang Quanhong Ping Xiaomei Liu Xing Xie Beihua Kong Ding Ma Weiguo Lu | 2022 | Frontiers of Medicine2022，16，2： | 2 |
| 14 | 2003–2017年东北露天生物质燃烧时空变化特征显示文摘近年来,露天生物质燃烧对我国东北重霾污染影响显著。本文综合利用MODIS C6火点产品以及下垫面类型产品,结合GFAS火点排放清单数据,定量研究了2003–2017年东北的火点演变特征。结果显示,东北区域近15年来火点震荡增加,并在2013–2017年达到峰值。火点季节变化明显,主要集中3–4月(占37%)和10–11月(占46%), 2013年之后春季火点突增,且在2016–2017年春季火点超过秋季。空间分布上,火点主要位于黑龙江(占71%),其次是吉林(16%)和辽宁(13%)。相比森林和草地下垫面类型,农田火点占比高达91%。本研究结果为东北区域露天生物质燃烧的重点监测时段和管控方案制订提供了参考依据。 | WANG Lili JIN Xin WANG Qinglu MAO Huiqin LIU Qiyang WENG Guoqing WANG Yuesi | 2020 | Atmospheric and Oceanic Science Letters2020，13，3： | 2 |
| 15 | Effects of short-term anoxic treatment on antioxidant ability and membrane integrity of postharvest kiwifruit during storage显示文摘 | Lili Song Haiyan Gao Hangjun Chen Jinlin Mao Yongjun Zhou Wenxuan Chen Yueming Jiang | 2008 | Food Chemistry2008，，： | 2 |
| 16 | 长期棉花秸秆还田和深松提高棉花产量的机理显示文摘我国有大量的滨海盐碱地资源尚未得到开发利用。优化滨海盐碱地改良和植棉技术有助于推动相关资源的开发利用。棉花秸秆还田和深松是常见的盐碱地土壤改良措施。其中,短期棉花秸秆还田可以提高滨海盐渍土棉花产量水平,深松可以切断土壤毛细管,抑制季节性返盐。 | 杨子山(编译) Zhang Le Mao Lili Yan Xiaoyu Liu Chengmin Song Xianliang Sun Xuezhen | 2022 | 中国棉花2022，49，12： | 2 |
| 17 | Comments on Chinese guidelines for diagnosis and treatment of melanoma 2018(English version)显示文摘Melanoma is a rare malignant tumor in China;however,its mortality rate is high and the incidence rate is also increasing year by year.In order to improve the efficacy of melanoma treatment,the National Health Commission of the People’s Republic of China revised Chinese guidelines for diagnosis and treatment of melanoma in 2018,and meanwhile its modified English version Chinese guidelines for diagnosis and treatment of melanoma 2018(English version)(1)is also published.Based on the most updated research findings for the Chinese ethnicity with emphasis on acral and mucosal melanomas,two most commonly seen subtypes in Asia,as well as treatment for melanoma liver metastases. | Xue Bai Lili Mao Jun Guo | 2019 | Chinese Journal of Cancer Research2019，31，5： | 1 |
| 18 | Crop growth, lightutilization and yield of relay intercropped cotton as affected by plant density and a plant growth reg- ulator显示文摘 | Mao Lili Zhang Lizhen Zhao Xinhua | 2014 | Field Crops Research2014，155，1： | 1 |
| 19 | A linear source method for soil infiltrability measurement and model representations显示文摘 | Mao Lili Lei Tingwu Li Xin | 2008 | Journal of Hydrology2008，353，12： | 1 |
| 20 | The molecular conformation of silk fibroin regulates osteogenic cell behavior by modulating the stability of the adsorbed protein-material interface显示文摘Silk fibroin(SF)can be used to construct various stiff material interfaces to support bone formation.An essential preparatory step is to partially transform SF molecules from random coils toβ-sheets to render the material water insoluble.However,the influence of the SF conformation on osteogenic cell behavior at the material interface remains unknown.Herein,three stiff SF substrates were prepared by varying theβ-sheet content(high,medium,and low).The substrates had a comparable chemical composition,surface topography,and wettability.When adsorbed fibronectin was used as a model cellular adhesive protein,the stability of the adsorbed protein-material interface,in terms of the surface stability of the SF substrates and the accompanying fibronectin detachment resistance,increased with the increasingβ-sheet content of the SF substrates.Furthermore,(i)larger areas of cytoskeleton-associated focal adhesions,(ii)higher orders of cytoskeletal organization and(iii)more elongated cell spreading were observed for bone marrow-derived mesenchymal stromal cells(BMSCs)cultured on SF substrates with high vs.lowβ-sheet contents,along with enhanced nuclear translocation and activation of YAP/TAZ and RUNX2.Consequently,osteogenic differentiation of BMSCs was stimulated on highβ-sheet substrates.These results indicated that theβ-sheet content influences osteogenic differentiation of BMSCs on SF materials in vitro by modulating the stability of the adsorbed protein-material interface,which proceeds via protein-focal adhesion-cytoskeleton links and subsequent intracellular mechanotransduction.Our findings emphasize the role of the stability of the adsorbed protein-material interface in cellular mechanotransduction and the perception of stiff SF substrates with differentβ-sheet contents,which should not be overlooked when engineering stiff biomaterials. | Yanlin Long Xian Cheng John A.Jansen Sander G.C.Leeuwenburgh Jing Mao Fang Yang Lili Chen | 2021 | Bone Research2021，9，2： | 1 |
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