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| 1 | A novel gene delivery system targeting cells expressing VEGF receptors显示文摘Two ligand oligopeptides GV1 and GV2 were designed according to the putative binding region of VEGF to its receptors. GV1, GV2 and endosome releasing oligopeptide HA20 were conjugated with poly-L-lysine or protamine and the resulting conjugates could interact with DNA in a noncovalent bond to form a complex. Using pSV2-β-galactosidase as a reporter gene, it has been demonstrated that exogenous gene was transferred into bovine aortic arch-derived endothelial cells (ABAE) andhuman malignant melanoma cell lines (A375) in vitro. In vivo experiments, exogenous gene was transferred into tumor vascular endothelial cells and tumor cells of subcutaneously transplanted human colon cancer LOVO, human malignant melanoma A375 and human hepatoma graft in nude mice. This system could also target gene to intrahepatically transplanted human hepatoma injected via portal vein in nude mice. These results are correlated with theGene delivery system targeting VEGF receptors relevant receptors (flt-1, flk-1/KDR) expression on the targeted cells and tissues. | LI JUN MIN JUN SONG HAN YI HUANG PEI KUN TIAN SHU MIN QU MIN YAO HUI QIU JIANG DA FANG WAN JING CHU LUO CHENG XIAO GU JIAN REN GU( National Labomtory for Oncogenes and Related Genes, Shanghai Cancer Institute, Shanghai 200032,China)(National Laboratory of | 1999 | Cell Research1999，9，1： | 22 |
| 2 | QTG-Seq Accelerates QTL Fine Mapping through QTL Partitioning and Whole-Genome Sequencing of Bulked Segregant Samples显示文摘Deciphering the genetic mechanisms underlying agronomic traits is of great importance for crop improvement. Most of these traits are controlled by multiple quantitative trait loci (QTLs), and identifying the underlying genes by conventional QTL fine-mapping is time-consuming and labor-intensive. Here, we devised a new method, named quantitative trait gene sequencing (QTG-seq), to accelerate QTL fine-mapping. QTGseq combines QTL partitioning to convert a quantitative trait into a near-qualitative trait, sequencing of bulked segregant pools from a large segregating population, and the use of a robust new algorithm for identifying candidate genes. Using QTG-seq, we fine-mapped a plant-height QTL in maize (Zea mays L.), qPH7, to a 300-kb genomic interval and verified that a gene encoding an NF-YC transcription factor was the functional gene. Functional analysis suggested that qPH7-encoding protein might influence plant height by interacting with a CO-like protein and an AP2 domain-containing protein. Selection footprint ana卜 ysis indicated that qPH7 was subject to strong selection during maize improvement. In summary, QTG-seq provides an efficient method for QTL fine-mapping in the era of “big data'. | Hongwei Zhang Xi Wang Qingchun Pan Pei Li Yunjun Liu Xiaoduo Lu Wanshun Zhong Minqi Li Linqian Han Juan Li Pingxi Wang Dongdong Li Yan Liu Qing Li Fang Yang Yuan-Ming Zhang Guoying Wang Lin Li | 2019 | Molecular Plant2019，12，3： | 13 |
| 3 | Piglets cloned from induced pluripotent stem cells显示文摘 | Nana Fan Jijun Chen Zhouchun Shang Hongwei Dou Guangzhen Ji Qingjian Zou Lu Wu Lixiazi He Fang Wang Kai Liu Na Liu Jianyong Han Qi Zhou Dengke Pan Dongshan Yang Bentian Zhao Zhen Ouyang Zhaoming Liu Yu Zhao Lin Lin Chongming Zhong Quanlei Wang Shouqi Wang Ying Xu Jing Luan Yu Liang Zhenzhen Yang Jing Li Chunxia Lu Gabor Vajta Ziyi Li Hongsheng Ouyang Huayan Wang Yong Wang Yang Yang Zhonghua Liu Hong Wei Zhidong Luan Miguel A Esteban Hongkui Deng Huanming Yang Duanqing Pei Ning Li Gang Pei Lin Liu Yutao Du Lei Xiao Liangxue Lai | 2013 | Cell Research2013，23，1： | 12 |
| 4 | Study on the physiological indices of Pinus sibirica and Pinus koraiensis seedlings under cold stress显示文摘Although Pinus sibirica and Pinus koraiensis are resistant to cold or low temperatures in the cold temperate regions of the northern hemisphere,the former has a stronger cold resistance.Research has been limited to the comparison of physiological responses of the two species to cold stress.In this study,5-year-old seedlings of P.sibirica and P.koraiensis were subjected to six temperature treatments,[20℃(control),0℃,-20℃,-40℃,-60℃,and-80℃],under different stress periods(6,12,24,and 48 h).The results showed that differences in each physiological index were significant between P.sibirica and P.koraiensis,except for the permeability of cell membranes,reactive oxygen species,proline and soluble proteins.An ANOVA test indicated that there were extreme differences among the temperatures for each index,stress time and temperature 9 time for most indices.All indices showed a similar trend for P.sibirica and P.koraiensis with decreasing temperature or the extension of stress time.Soluble sugars and proline increased at 0 to-20℃and then remained unchanged with temperature decline.Other indices showed an increase from 20 to-20℃,stable from-20 to-40℃and a decrease from-40 to-80℃.All the indices increased and then declined along with the prolonged cold stress time,except for the control.From 0 to-40℃,the permeability of cell membranes,relative conductivity,reactive oxygen species and malonaldehyde of P.koraiensis seedlings were higher than in P.sibirica,but superoxide dismutase,peroxidase,catalase activity and soluble sugars,proline,and soluble proteins content emerged as opposite.This study compared the physiological mechanism responses to cold stress between P.sibirica and P.koraiensis to provide the basis for the introduction,distribution,and genetic improvement of these coniferous species. | Fang Wang Deyang Liang Xiaona Pei Qinhui Zhang Peng Zhang Jianqiu Zhang Zhimin Lu Yuchun Yang Guifeng Liu Xiyang Zhao | 2019 | Journal of Forestry Research2019，30，4： | 12 |
| 5 | Effect of dynamic hip screw on the treatment of femoral neck fracture in the elderly显示文摘ObjectiveTo 讨论指示,外科的过程,并且在在 elderly.MethodsA 的大腿骨的颈骨折的治疗的动态新潮的螺丝钉( DHS )的药品效果回顾的学习被进行分析在我们在2009年6月和2011年11月之间的部门与 DHS 为大腿骨的颈骨折被对待的 42 个老病人的临床的数据。与 68.5 年(范围 60-75 年) 的吝啬的年龄有 21 男性和 21 女性。根据花园分类,有类型 II,类型 III 的 21 个盒子和类型 IV 破裂的 2 个盒子的 19 个盒子。由 Singh 索引分类,有水平 2 的 3 个盒子,水平 4 破裂的水平 3 和 20 盒子的 19 个盒子。在手术以后的哈里斯标准,复杂并发症和功能恢复是在 42 个病人的 analysed.ResultsThe 一般水准住院时间是 11.2 天(范围 7-21 天) 。所有病人被跟随在上面为 12-26 月(平均数 18 月) 。没有肺感染,深静脉的血栓或另外的复杂并发症发生了。螺丝钉的部分支持外面在 2 种情况中被发现。内部固定设备在骨折愈合以后被撤退。内部固定切在 1 种情况中被发现,并且他在全部的新潮的关节造形术以后有好恢复。骨折愈合的时间从 3-6 月(一般水准 4.5 月)。根据哈里斯标准, 15 个盒子作为优秀被评估, 24 好, 2 交易会和 1 个穷人。哈里斯规模显著地从 30.52 被改进; ± ; 2.71 外科手术前地到 86.61 ; ± ; 2.53 在 6 个月手术后地( P ;< ; 0.05 ) .ConclusionDHS ,是最小的侵略,允许早活动并且忍受重量,为有大腿骨的颈骨折的老病人的治疗是明智的。另外,它能避免在人工的联合代替看见的复杂并发症。它对有温和骨质疏松症的病人特别合适。 | Zhao Wenbo Liu Lei Zhang Hui Fang Yue Pei Fuxing Yang Tianfu | 2014 | Chinese Journal of Traumatology2014，17，2： | 11 |
| 6 | Passive-source ocean bottom seismograph(OBS) array experiment in South China Sea and data quality analyses显示文摘Long-term passive source ocean bottom seismograph(OBS) observatory is challenging due to various technical difficulties. In order to gain experience in this field, and to reveal the lithospheric structure beneath the extinct ridge in the central South China Sea(SCS), we carried out a passive source OBS array experiment, which includes 18 OBSs, in the deep portion of SCS. Here we present the instrumentation, the OBS deployment and recovery of this experiment, and more importantly, the data quality evaluated by a number of approaches. Through processing and inspecting waveforms from global, regional and local earthquakes, we find that most of recovered OBSs have good data quality with discernible main phases. The ambient noise analyses of OBS recordings show that their noise is higher than the global average, and the horizontal component is noisier than the vertical, indicating current impacts on horizontal components are more severe. In the period range of 5–10 s, there is a noise notch for the SCS OBSs, and noise levels of horizontal components are comparable to the vertical. This feature, which is not seen at OBS stations in open ocean, suggests the distant sources for double frequency microseism in this marginal sea are not significant. In addition, we successfully determined the orientations for 7 OBSs by investigating their Rayleigh wave polarizations; and we demonstrated the dispersion feature of Rayleigh waves through the frequency-time analysis. Finally, we summarized lessons learned from this experiment regarding the passive source OBS investigations in SCS. | Chenguang Liu Qingfeng Hua Yanliang Pei Ting Yang Shaohong Xia Mei Xue Ba Manh Le Da Huo Fang Liu Haibo Huang | 2014 | Chinese Science Bulletin2014，59，33： | 11 |
| 7 | A critical review on the health promoting effects of mushrooms nutraceuticals显示文摘Extensive elucidations focusing on the efficient health promoting properties and high nutritional values of mushrooms have been expanded dynamically from the past few decades.Due to its high quality of proteins,polysaccharides,unsaturated fatty acids,mineral substances,triterpenes sterols and secondary metabolites,mushrooms have always been appreciated for their vital role in protecting and curing various health problems,such as immunodeficiency,cancer,inflammation,hypertension,hyperlipidemia,hypercholesterolemia and obesity.Moreover,investigations in recent years have revealed the correlations between the health-promoting benefits and gut microbiota regulating effects induced by the mushrooms intake.Researches on the immense role in the nutritional and health benefits displayed by mushrooms have become an emergent task to study.The present article overviewed and compiled the latest information correlated to the health benefits and underlying functional mechanisms of mushroom nutraceuticals,and concluded that the supplementation of mushrooms as a dietary composition could become a natural adjuvant for the prevention and treatment of several health diseases. | Gaoxing Ma Wenjian Yang Liyan Zhao Fei Pei Donglu Fang Qiuhui Hu | 2018 | Food Science and Human Wellness2018，7，2： | 10 |
| 8 | Targeting redox-altered plasticity to reactivate synaptic function: A novel therapeutic strategy for cognitive disorder显示文摘Redox-altered plasticity refers to redox-dependent reversible changes in synaptic plasticity via altering functions of key proteins, such as N-methyl-D-aspartate receptor(NMDAR). Age-related cognitive disorders includes Alzheimer’s disease(AD), vascular dementia(VD), and age-associated memory impairment(AAMI). Based on the critical role of NMDAR-dependent long-term potentiation(LTP) in memory, the increase of reactive oxygen species in cognitive disorders, and the sensitivity of NMDAR to the redox status, converging lines have suggested the redox-altered NMDAR-dependent plasticity might underlie the synaptic dysfunctions associated with cognitive disorders. In this review, we summarize the involvement of redox-altered plasticity in cognitive disorders by presenting the available evidence. According to reports from our laboratory and other groups, this 'redox-altered plasticity' is Hydrogen sulfidemore similar to functional changes rather than organic injuries, and strategies targeting redox-altered plasticity using pharmacological agents might reverse synaptic dysfunctions and memory abnormalities in the early stage of cognitive disorders. Targeting redox modifications for NMDARs may serve as a novel therapeutic strategy for memory deficits. | Pei Wang Fang Wang Lan Ni Pengfei Wu Jianguo Chen | 2021 | Acta Pharmaceutica Sinica B2021，11，3： | 8 |
| 9 | Interleukin-17A gene variants and risk of coronary artery disease:a large angiography-based study显示文摘Background Recent studies have also revealed that interleukin(IL)-17A plays a key role in atherosclerosis and its complication,but the relationship of its common variants with coronary artery disease(CAD) has not been extensively studied.Methods We systematically screened sequence variations in the IL17A gene and designed an angiog-raphy -based case-controlled study consisting of 1031 CAD patients and 935 control subjects to investigate the association between the selected polymorphisms of IL-17A gene and CAD risk in Chinese Han population.Results Frequencies of IL17A rs8193037 GG homozygote and G allele were significantly higher in the patient group than those in the control group(P<0.001;OR=0.68;95%CI=0.54-0.85).Stratification analysis showed that the IL17A rs8193037 G allele significantly increased the risk of CAD only among male subjects (P=0.001;OR=0.63;95%CI=0.47-0.83).After adjustment for conventional risk factors,binary logistic regression analysis showed that the G allele carriers(GG +AG) had significantly increased CAD risk compared with the AA homozygotes (adjusted P<0.001;OR 0.43;95%CI,0.33- 0.58).ELISA showed augmented IL17A production in plasma of the AMI patients.Conclusions Based on our data,we speculated that the SNP rs8193037 of IL17A gene is significantly associated with CAD risk in Chinese Han population and the rs8193037 G allele which is associated with increased expression of IL17A in AMI patients may be an independent predictive factor for CAD. | ZHANG Xiao-lin,PEI Fang,HAN Ya-Ling,YAN Cheng-Hui, HUANG Ming-Fang,WANG Tao (Department of Cardiology,Cardiovascular Institute of PLA, Shenyang Northern Hospital,Shenyang 110031,China) | 2011 | 岭南心血管病杂志2011，17，S1： | 8 |
| 10 | Ultrasonographic Feature of Persistent Hyperplastic Primary Vitreous显示文摘Purpose:.To observe the ultrasonographic features of patients with persistent hyperplastic primary vitreous(PHPV).Methods: Thirty-two subjects(34 eyes) diagnosed with PHPV were evaluated by ultrasonography.Results:.The ultrasonography demonstrated a retrolental mass extending from the optic disc to the posterior lens capsule,manifested as band,.regular triangle,.or inverted triangle shapes..The band-shaped echo was characterized as a linear band extended from the optic disc to the posterior lens capsule..The regular triangle-shaped echo was manifested as a membranous septum with a wide base extended from the optic disc to the posterior lens capsule, and the anterior part became narrower..The inverted triangle echo was characterized as a membranous septum with a narrow base extended from the optic disc to the posterior lens capsule,.and the anterior part become wider.Conclusion:.Ultrasonography is noninvasive and safe,.and can offer real-time display of intraocular structures. This is especially important in individuals who are uncooperative or unsuitable for fundus examination due to media opacity. Combined with clinical feature, ultrasonography provides vital evidence for the diagnosis of PHPV..Thought observing ultrasonographic feature, clinicians could evaluate the size, position and severity of lesions in PHPV patients,.and which would be helpful to determine the surgical approach and clini-cal prognosis. | Andina Hu Miner Yuan Fang Liu Yu Yang Xiaoyan Ding Xiaoqing Pei | 2014 | Eye Science2014，29，2： | 8 |
| 11 | Global transcriptome analysis for identification of interactions between coding and noncoding RNAs during human erythroid differentiation显示文摘在 erythroid 开发期间编码基因, miRNAs,和 lncRNAs 上的研究在最近的年里被执行了。然而,集中于三种 RNA 类型的集成的分析还得被做。在现在的学习,我们比较了编码基因, miRNA,和 lncRNA 表示侧面的动力学。为了在在 transcriptome 控制这些变化的红血球生成和潜在的机制探索动态变化,铺平,我们利用了定序技术从绳索血获得 transcriptome 数据的高产量造血的干细胞和下列四个 erythroid 区别阶段,以及从成熟的红血房间。结果显示 lncRNAs 为 erythroid 区别正在答应房间标记候选人。聚类分析分类差别表示基因进在 stemness 维护期间对应于动态变化的四种子类型,中间区别,并且成熟。综合分析揭示了 RNA 潜在地参予了控制血细胞成熟的那 noncoding,并且特别与到氧种类和 DNA 损坏的 heme 新陈代谢和回答联系了。这些规章的相互作用在一个全面网络,在 RNA 之间的从而推断的关联和他们的联系功能被显示。这些数据为正常红血球生成的学习提供了一个大量的资源,它将允许 erythroid 开发的进一步的调查和理解并且获得 erythroid 混乱。 | Nan Ding Jiafei Xi Yanming Li Xiaoyan Xie Jian Shi Zhaojun Zhang Yanhua Li Fang Fang Sihan Wang Wen Yue Xuetao Pei Xiangdong Fang | 2016 | Frontiers of Medicine2016，10，3： | 6 |
| 12 | High Levels of Hepatitis B Surface Antigen Increase Risk of Hepatocellular Carcinoma in Patients With Low HBV Load显示文摘 | Tai–Chung Tseng Chun–Jen Liu Hung–Chih Yang Tung–Hung Su Chia–Chi Wang Chi–Ling Chen Stephanie Fang–Tzu Kuo Chen–Hua Liu Pei–Jer Chen Ding–Shinn Chen Jia–Horng Kao | 2012 | Gastroenterology2012，，5： | 6 |
| 13 | In vitro differentiation of human adipose-derived mesenchymal stem cells into endothelial-like cells显示文摘The neovascularization of ischemic tis- sue is a crucial initial step for the functional rehabili- tation and wound healing. However, the short of seed cell candidate for the foundation of vascular network is still a big issue. Human adipose tissue derived mesenchymal stem cells (hADSCs), which possess multilineage potential, are capable of adipogenic, osteogenic, and chondrogenic differentiation. We examined whether this kind of stem cells could dif- ferentiate into endothelial-like cells and participate in blood vessel formation, and whether they could be used as an ideal cell source for therapeutic angio- genesis in ischemic diseases or vascularization of tissue constructs. The results showed that hADSCs, grown under appropriately induced conditions, dis- played characteristics similar to those of vessel en- dothelium. The differentiated cells expressed endo- thelial cell markers CD34 and vWF, and had high metabolism of acetylated low-density lipoprotein and prostacyclin. In addition, the induced cells were able to form tube-like structures when cultured on matrigel. Our data indicated that induced hADSCs could ex- hibit characteristics of endothelial cells. Therefore, these cells, as a source of human endothelial cells, may find many applications in such realms as engi- neering blood vessels, endothelial cell transplantation for myocardial regeneration, and induction of angio- genesis for treatment of regional ischemia. | GUAN Lidong LI Shaoqing WANG Yunfang YUE Huimin LIU Daqing HE Lijuan BAI Cixian YAN Fang NAN Xue SHI Shuangshuang PEI Xuetao | 2006 | Chinese Science Bulletin2006，51，15： | 6 |
| 14 | Phytosterol content and the campesterol:sitosterol ratio influence cotton fiber development: role of phytosterols in cell elongation显示文摘Phytosterols play an important role in plant growth and development,including cell division,cell elongation,embryogenesis,cellulose biosynthesis,and cell wall formation.Cotton fiber,which undergoes synchronous cell elongation and a large amount of cellulose synthesis,is an ideal model for the study of plant cell elongation and cell wall biogenesis.The role of phytosterols in fiber growth was investigated by treating the fibers with tridemorph,a sterol biosynthetic inhibitor.The inhibition of phytosterol biosynthesis resulted in an apparent suppression of fiber elongation in vitro or in planta.The determination of phytosterol quantity indicated that sitosterol and campesterol were the major phytosterols in cotton fibers;moreover,higher concentrations of these phytosterols were observed during the period of rapid elongation of fibers.Furthermore,the decrease and increase in campesterol:sitosterol ratio was associated with the increase and decease in speed of elongation,respectively,during the elongation stage.The increase in the ratio was associated with the transition from cell elongation to secondary cell wall synthesis.In addition,a number of phytosterol biosynthetic genes were down-regulated in the short fibers of ligon lintless-1mutant,compared to its near-isogenic wild-type TM-1.These results demonstrated that phytosterols play a crucial role in cotton fiber development,and particularly in fiber elongation. | Shasha Deng Ting Wei Kunling Tan Mingyu Hu Fang Li Yunlan Zhai Shue Ye Yuehua Xiao Lei Hou Yan Pei Ming Luo | 2016 | Science China(Life Sciences)2016，59，2： | 5 |
| 15 | Efficient synthesis and in vitro antifungal activity of 1H-benzimidazol-l-yl acetates/propionates containing 1H-1,2,4-triazole moiety显示文摘A series of novel 1H-benzimidazol-1-yl acetates and 1H -benzimidazol-1-yl propionates containing 1H-1,2,4-triazole moiety were synthesized under microwave irradiation by multi-step reactions,in yields of 87-94%.Their in vitro antifungal activities against Botrytis cinerea and Sclerotinia sclerotiorum were evaluated by mycelial growth rate method.All the target compounds exhibit high activities against B.cinerea with the EC50 values of 7.96-21.74μg/mL,higher than that of carbendazim. | Pei Zhi Zhang Shao Fang Zhou Tian Ren Li Lin Jiang | 2012 | Chinese Chemical Letters2012，23，12： | 5 |
| 16 | Timing of internal fixation and effect on Schatzker IV-VI tibial plateau fractures显示文摘内部固定的效果在 Schatzker IV-VI 胫骨的高原破裂的 42 个盒子的临床的数据在我们的部门对待的 Schatzker IV-VI 胫骨的高原 fractures.MethodsThe 的治疗学的结果上在不同时间执行了的 ObjectiveTo 学习回顾地被分析。在这 42 个病人之中, 21 在 12 以内接受了外科的治疗;在损害以后的 h (组我) ,另外的 21 被在胀大的软织物减退了以后,推迟的内部固定跟随的拖拉或灰浆固定(组 II ) 首先对待。外科的时间,复杂并发症,一些医院停留,住院的费用,和骨折联合的时间,以及功能的恢复被分析并且在二 groups.ResultsAfter 10-28 月后续(平均数 16.5 月)之间比较了,除了 5 个盒子输了到后续,没有差别在后续的结束关于外科的时间,外科手术前、手术后的复杂并发症,特殊外科( HSS )分数的愈合的时间或医院在二个组之间被发现。 | TANG Xin LIU Lei TU Chong-qi YANG Tian-fu WANG Guang-lin FANG Yue LI Jian LI Qi PEI Fu-xing | 2012 | Chinese Journal of Traumatology2012，15，2： | 5 |
| 17 | Preparation and Properties of Asphalt Modified with a Composite Composed of Waste Package Poly(vinyl chloride) and Organic Montmorillonite显示文摘A composite composed of waste package poly(vinyl chloride)(WPVC) and organic montmorillonite(OMMT) was prepared by coextrusion, which is used for modifying asphalt. The micromorphology of the WPVC/OMMT composite and its effect on the macroscopic properties of asphalt were studied using a fluorescent microscope and an X-ray diffractometer(XRD). The introduction of OMMT allows the WPVC to be good dispersed in the asphalt matrix, as demonstrated by differential scanning calorimetry(DSC) and Fourier transform infrared spectroscopy(FTIR) analysis. The results indicate that asphalt modified by WPVC/OMMT composites with low OMMT content results in better high-temperature storage stability and physical properties of modified asphalt. | Changqing Fang Xiaolong Liu Ruien Yu Pei Liu Wanqing Lei | 2014 | Journal of Materials Science & Technology2014，30，12： | 5 |
| 18 | Outbreaks of Acute Respiratory Disease Associated with Human Adenovirus Infection in Closed Camps,China,December 2011–March 2014显示文摘Summary What is already known on this topic?Human adenovirus(HAdV)was frequently associated with acute respiratory disease(ARD)outbreaks in military camps.What is added by this report?HAdV-B7,HAdV-B14,and HAdV-B55 were determined to be responsible for 3,2,and 4 ARD outbreaks in military camps,China,respectively,with a total attack rate of 28.0%during 2011 to 2014. | Juan Du Xiaodong Zhao Fang Tang Doudou Huang Guangqian Pei Xiaoai Zhang Baogui Jiang Qingbin Lu Wei Liu Yigang Tong | 2021 | China CDC weekly2021，3，38： | 4 |
| 19 | Construction and Secretory Expression of β-Galactosidase Gene from Lactobacillus Bulgaricus in Lactococcus Lactis显示文摘Objective This study is to examine the secretion effects of β-galactosidase in Lactococcus lactis.Methods The usp45 and β-galactosidase genes were cloned and inserted into plasmid pMG36e to obtain the recombinant plasmid pMG36e-usp-lacZ.This recombinant plasmid was transformed into both Escherichia coli DH5α and L.lactis MG1363.The enzyme activity,gene sequencing,SDS-PAGE and hereditary stability were assessed and studied.Results The lacZ gene inserted into plasmids pMG36e-usp-lacZ was 99.37% similar to the GenBank sequence,and SDS-PAGE revealed an evident idio-strap at 116 KDa between L.lactis MG1363/pMG36eusp-lacZ in both supernatant and cell samples.β-Galactosidase activity measured 0.225 U/mL in L.lactis pMG36e-usp-lacZ transformants,and its secretion rate was 10%.The plasmid pMG36e-usp-lacZ appeared more stable in MG1363.Conclusion The authors concluded that these new recombinant bacteria well expressed and secreted β-galactosidase,indicating that the β-galactosidase expression system was successfully constructed,and this might provide a new solution for management of lactose intolerance specifically and promote the use of gene-modified organisms as part of the food-grade plasmid in general. | ZHANG Wen WANG Chuan HUANG Cheng Yu YU Qian LIU Heng Chuan ZHANG Chao Wu PEI Xiao Fang | 2012 | Biomedical and Environmental Sciences2012，25，2： | 4 |
| 20 | Sex-specific and Dose-response Relationship between the Incidence of Gallstones and Components of the Metabolic Syndrome in Jinchang Cohort:A Prospective Study显示文摘Gallstones is a very common gastrointestinal disease,involving the formation of masses in the gall bladder or biliary tract,due to abnormally high concentrations of cholesterol or bilirubin in the bile[1].There is a 10%–20%prevalence of gallstones worldwide,and>20%of patients with gallstones show clinical symptoms,implying a substantial disease burden[1]. | YANG Jing Li HUANG Jun Jun CHENG Ning ZHANG De Sheng LIU Si Min HUANG Wen Ya LI Na HUANG Pei Yao DING Jiao LIU Nian BAO Kai Fang DING Jie CHEN Xiao Liang ZHENG Tong Zhang BAI Ya Na | 2020 | Biomedical and Environmental Sciences2020，33，8： | 4 |
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