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| 1 | The Qitai radio telescope显示文摘This study presents a general outline of the Qitai radio telescope(QTT)project.Qitai,the site of the telescope,is a county of Xinjiang Uygur Autonomous Region of China,located in the east Tianshan Mountains at an elevation of about 1800 m.The QTT is a fully steerable,Gregorian-type telescope with a standard parabolic main reflector of 110 m diameter.The QTT has adopted an umbrella support,homology-symmetric lightweight design.The main reflector is active so that the deformation caused by gravity can be corrected.The structural design aims to ultimately allow high-sensitivity observations from 150 MHz up to115 GHz.To satisfy the requirements for early scientific goals,the QTTwill be equipped with ultra-wideband receivers and large field-of-view multi-beam receivers.A multi-function signal-processing system based on RFSo C and GPU processor chips will be developed.These will enable the QTT to operate in pulsar,spectral line,continuum and Very Long Baseline Interferometer(VLBI)observing modes.Electromagnetic compatibility(EMC)and radio frequency interference(RFI)control techniques are adopted throughout the system design.The QTT will form a world-class observational platform for the detection of lowfrequency(nano Hertz)gravitational waves through pulsar timing array(PTA)techniques,pulsar surveys,the discovery of binary black-hole systems,and exploring dark matter and the origin of life in the universe.The QTT will also play an important role in improving the Chinese and international VLBI networks,allowing high-sensitivity and high-resolution observations of the nuclei of distant galaxies and gravitational lensing systems.Deep astrometric observations will also contribute to improving the accuracy of the celestial reference frame.Potentially,the QTT will be able to support future space activities such as planetary exploration in the solar system and to contribute to the search for extraterrestrial intelligence. | Na Wang Qian Xu Jun Ma Zhiyong Liu Qi Liu Hailong Zhang Xin Pei Maozheng Chen Richard N.Manchester Kejia Lee Xingwu Zheng Hans J.Kärcher Wulin Zhao Hongwei Li Dongwei Li Martin Süss Matthias Reichert Zhongyi Zhu Congsi Wang Mingshuai Li Rui Li Ning Li Guljaina Kazezkhan Wenming Yan Gang Wu Lang Cui Ming Zhang Haitao Li | 2023 | Science China(Physics,Mechanics & Astronomy)2023，66，8： | 14 |
| 2 | Efficient generation of transgenic chickens using the spermatogonial stem cells in vivo and ex vivo transfection显示文摘The highly efficient novel methods to produce transgenic chickens were established by directly in-jecting the recombinant plasmid containing green fluorescent protein (GFP) gene into the cock's testis termed as testis-medianted gene transfer (TMGT), and transplanting transfected spermatogonial stem cells (TTSSCs). For the TMGT approach,four dosages of pEGFP-N1 DNA/cationic polymer complex were injected intratesticularly. The results showed: (1) 48 h after the injection,the percentages of testis cells expressing GFP were 4.0%, 8.7%, 10.2% and 13.6% in the 50, 100, 150 and 200 μg/mL group, re-spectively. The difference from the four dosage groups was significant (P<0.05). On day 25 after the injection, a dosage-dependent and time-dependent increase in the number of transgenic sperm was observed. The percentages of gene expression reached the summit and became stable from day 70 to 160, being 12.7%, 12.8%, 15.9% and 19.1%, respectively. The difference from the four dosage groups was also significant (P<0.05). (2) 70 d after the injection, strong green fluorescent could be observed in the seminiferous tubules by whole-mount in-situ hybridization. (3) 70 d after the injection, the semen was collected and used to artificially inseminate wild-type females. The blastoderms of F1 and F2 transgenic chicken expressed GFP were 56.2% (254/452) and 53.2% (275/517), respectively. The detec-tion of polymerase chain reaction (PCR) of F1 and F2 transgenic chicken blood genomic DNA showed that 56.5% (3/23) of F1 and 52.9% (9/17) of F2 were positive. Southern blot showed GFP DNA was in-serted in their genomic DNAs. (4) Frozen whole mount tissue sections of F1 and F2 transgenic chicken liver, heart, kidney and muscle showed that the rates of green fluorescent positive were between 50.0% and 66.7%. (5) With the TTSSCs method, SSCs ex vivo transfected with GFP were transplanted into recipient roosters whose endogenic SSCs had been resoluted. The donor SSCs settled and GFP ex-pression became readily detectable in the frozen whole mount tissue sections of recepient testes. Moreover, sperms carrying GFP could be produced normally. The results of artificially inseminating wild-type females with these sperms showed 12.5% (8/64) of offspring embryo expressed GFP and 11.1% (2/18) hatched chicks were tested transgenic. Our data therefore suggest TMGT and TTSSCs are the feasible methods for the generation of transgenic chickens. | LI BiChun SUN GuoBo SUN HuaiChang XU Qi GAO Bo ZHOU GuanYue ZHAO WenMing WU XinSheng BAO WenBin YU Fei WANG KeHua CHEN GuoHong | 2008 | Science China(Life Sciences)2008，51，8： | 13 |
| 3 | Genetic analysis and fine mapping of an enclosed panicle mutant esp2 in rice (Oryza sativa L.)显示文摘The phenomenon of panicle enclosure in rice is mainly caused by the shortening of uppermost internode.Elucidating the molecular mechanism of panicle enclosure will be helpful for solving the problem of panicle enclosure in male sterile lines and creating new germplasms in rice.We acquired a monogenic recessive enclosed panicle mutant,named as esp2 (enclosed shorter panicle 2),from the tissue culture progeny of indica rice cultivar Minghui-86.In the mutant,panicles were entirely enclosed by flag leaf sheaths and the uppermost internode was almost completely degenerated,but the other internodes did not have obvious changes in length.Genetic analysis indicated that the mutant phenotype was controlled by a recessive gene,which could be steadily inherited and was not affected by genetic background.Apparently,ESP2 is a key gene for the development of uppermost internode in rice.Using an F 2 population of a cross between esp2 and a japonica rice cultivar Xiushui-13 as well as SSR and InDel markers,we fine mapped ESP2 to a 14-kb region on the end of the short arm of chromosome 1.According to the rice genome sequence annotation,only one intact gene exists in this region,namely,a putative phosphatidylserine synthase gene.Sequencing analysis on the mutant and the wild type indicated that this gene was inserted by a 5287-bp retrotransposon sequence.Hence,we took this gene as a candidate of ESP2.The results of this study will facilitate the cloning and functional analysis of ESP2 gene. | GUAN HuaZhong DUAN YuanLin LIU HuaQing CHEN ZhiWei ZHUO Ming ZHUANG LiJun QI WenMing PAN RunSen MAO DaMei ZHOU YuanChang WANG Feng WU WeiRen | 2011 | Chinese Science Bulletin2011，56，14： | 6 |
| 4 | Fine mapping of the rice bacterial blight resistance gene Xa-4 and its co-segregation marker显示文摘An F2 population developed from the Xa-4 near isogenic lines, IR24 and IRBB4, was used for fine mapping of the rice bacterial blight resistance gene, Xa-4. Some restriction fragment length polymorphism (RFLP) markers on the high-density map constructed by Harushima et al. and the amplified DMA fragments homologous to the conserved domains of plant disease resistance (R) genes were used to construct the genetic linkage map around the gene Xa-4 by scoring susceptible individuals in the population. Xa-4 was mapped between the RFLP marker G181 and the polymerase chain reaction (PCR) marker M55. The R gene homologous fragment marker RS13 was found co-segregating with Xa-4 by analyzing all the plants in the population. This result opened an approach to map-based cloning of this gene, and marker RS13 can be applied to molecular marker-assisted selection of Xa-4 in rice breeding programs. | WANG Wenming ZHOU Yongli JIANG Guanhuai MA Bojun CHEN Xuewei ZHANG Qi ZHU Lihuang ZHAI Wenxue | 2000 | Chinese Science Bulletin2000，45，19： | 4 |
| 5 | Molecular cloning and expression patterns of the cholesterol side chain cleavage enzyme(CYP11A1) gene during the reproductive cycle in goose(Anas cygnoides)显示文摘Background: CYP11A1, a gene belonging to the family 11 of cytochrome P450, encodes a crucial steroidogenic enzyme that catalyzes the initial step in the production of all classes of steroids. Many studies show that CYP11A1 plays a role in ovary function. However, the role of CYP11A1 in goose reproductive cycle remains largely unknown.Results: In this study, full-length CYP11A1 c DNA of Zhedong goose was obtained using reverse transcription polymerase chain reaction(RT-PCR) and rapid amplification of c DNA ends(RACE). The c DNA consisted of a 96-base pair(bp) 5′untranslated region(UTR), a 179-bp 3′UTR and a 1509-bp open reading frame. The open reading frame encodes a putative 503 amino acid protein that shares high homology with CYP11A1 of other birds. The amino acid sequence possesses conserved domains of the P450 superfamily, which include the steroid-binding domain and the heme-binding region. Real-time quantitative polymerase chain reaction(q PCR) analysis revealed CYP11A1 mR NA was expressed ubiquitously in every Zhedong goose tissue analyzed, including the heart, liver, glandular stomach,lung, spleen, kidney, intestinum tenue, intestinum crassum, cerebrum, cerebellum, muscle, oviduct, pituitary,hypothalamus and ovary.. The relatively low levels of CYP11A1 m RNA were detected in pituitary, ovary and oviduct tissues at ovulation when compared with levels at oviposition. Interestingly, higher expression was observed in ovary and oviduct tissues during brooding. Lastly, higher m RNA expression of Yangzhou geese was detected during the ovulation period than that of Zhedong geese.Conclusions: Our findings reveal the sequence characterization and expression patterns of the CYP11A1 gene during the goose reproductive cycle, which may provides correlative evidence that CYP11A1 expression is important in reproduction activity. | Qi Xu Yadong Song Yang Chen Ran Liu Yang Zhang Yang Li Zhengyang Huang Wenming Zhao Guobin Chang Guohong Chen | 2016 | Journal of Animal Science and Biotechnology2016，7，2： | 2 |
| 6 | Population Genetics of SARS-CoV-2: Disentangling Effects of Sampling Bias and Infection Clusters显示文摘A novel RNA virus,the severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),is responsible for the ongoing outbreak of coronavirus disease 2019(COVID-19).Population genetic analysis could be useful for investigating the origin and evolutionary dynamics of COVID-19.However,due to extensive sampling bias and existence of infection clusters during the epidemic spread,direct applications of existing approaches can lead to biased parameter estimations and data misinterpretation.In this study,we first present robust estimator for the time to the most recent common ancestor(TMRCA)and the mutation rate,and then apply the approach to analyze 12,909 genomic sequences of SARS-CoV-2.The mutation rate is inferred to be 8.69×10^(−4) per site per year with a 95%confidence interval(CI)of[8.61×10^(−4),8.77×10^(−4)],and the TMRCA of the samples inferred to be Nov 28,2019 with a 95%CI of[Oct 20,2019,Dec 9,2019].The results indicate that COVID-19 might originate earlier than and outside of Wuhan Seafood Market.We further demonstrate that genetic polymorphism patterns,including the enrichment of specific haplotypes and the temporal allele frequency trajectories generated from infection clusters,are similar to those caused by evolutionary forces such as natural selection.Our results show that population genetic methods need to be developed to efficiently detangle the effects of sampling bias and infection clusters to gain insights into the evolutionary mechanism of SARS-CoV-2.Software for implementing VirusMuT can be downloaded at https://bigd.big.ac.cn/biocode/tools/BT007081. | Qi Liu Shilei Zhao Cheng-Min Shi Shuhui Song Sihui Zhu Yankai Su Wenming Zhao Mingkun Li Yiming Bao Yongbiao Xue Hua Chen | 2020 | Genomics, Proteomics & Bioinformatics2020，18，6： | 1 |
| 7 | Room temperature interfacial reactions in electrodeposited Au/Sn couples显示文摘 | Wenming Tang Anqiang He Qi Liu Douglas G. Ivey | 2008 | Acta Materialia2008，，19： | 1 |
| 8 | Microstructures and mechanical properties of T92/Super304H dissimilar steel weld joints after high-temperature ageing显示文摘 | Guohong Chen Qi Zhang Junjian Liu Jiaqing Wang Xinhai Yu Jian Hua Xiaolong Bai Tao Zhang Jianhua Zhang Wenming Tang | 2013 | Materials and Design2013，，： | 1 |
| 9 | Fabrication and microstructures of sequentially electroplated Au-rich eutectic Au/Sn alloy solder显示文摘 | Tang Wenming He Anqiang Liu Qi | 2008 | Journal of Materials Sci- ence : Materials in Electronics2008，19，12： | 1 |
| 10 | Room temper- ature interracial reactions in electrodeposited Au/Sn couples 显示文摘 | Tang Wenming He Anqiang Liu Qi | 2008 | ActaMaterialia2008，56，： | 1 |
| 11 | Deinterleaving of radar pulse based on implicit feature显示文摘In the complex countermeasure environment,the pulse description words(PDWs)of the same type of multi-function radar emitters are similar in multiple dimensions.Therefore,it is difficult for conventional methods to deinterleave such emitters.In order to solve this problem,a pulse deinterleaving method based on implicit features is proposed in this paper.The proposed method introduces long short-term memory(LSTM)neural networks and statistical analysis to mine new features from similar PDW features,that is,the variation law(implicit features)of pulse sequences of different radiation sources over time.The multi-function radar emitter is deinterleaved based on the pulse sequence variation law.Statistical results show that the proposed method not only achieves satisfactory performance,but also has good robustness. | GUO Qiang TENG Long WU Xinliang QI Liangang SONG Wenming | 2023 | Journal of Systems Engineering and Electronics2023，34，6： | 0 |
| 12 | Co-Ni Electromagnetic Coupling in Hollow Mo_(2)C/NC Sphere for Enhancing Electromagnetic Wave Absorbing Performance显示文摘For enhancing the electromagnetic wave(EW)attenuation and adsorption,rational constructing and homogeneously distributing bimetallic electromagnetic coupling units in hollow structure is an effective way,but hard to achieve.Herein,a CoNi-doped hybrid zeolite imidazole framework was synthesized as precursor,which was further converted into a hollow CoNi-bimetallic doped molyb-denum carbide sphere(H-CoNi@MoC/NC)through a two-step etching and calcination strategy.At the loading amount of 15 wt%,a strong absorption of minimum reflection loss(RL_(min))of-60.05 dB at 7.2 GHz with the thickness of 3.1 mm and a wide effective ad-sorption bandwidth(EAB)of 3.52 GHz at the thickness of 2.5 mm were achieved,which was far beyond the reported MoC-based metallic hybrids.The crucial synergistic Co-Ni electromagnetic coupling effect in the composite was characterized,not only enhanc-ing the dipolar/interfacial polarization,but also promoting the impedance matching,displaying the optimized EW absorbing perfor-mance. | Xiufang Yang Wenming Gao Jiamin Chen Xing Lu Dong Yang Yifan Kang Qi Liu Yuchang Qing Wenhuan Huang | 2023 | Chinese Journal of Chemistry2023，41，1： | 0 |
| 13 | Metagenomic evidence for the co-existence of SARS and H1N1 in patients from 2007-2012 flu seasons in France显示文摘By re-analzying public metagenomic data from 101 patients infected with influenza A virus during the 2007–2012 H1N1 flu seasons in France,we identified 22 samples with SARS-CoV sequences.In three of them,the SARS genome sequences could be fully assembled out of each.These sequences are highly similar(99.99%and 99.70%)to the artificially constructed recombinant SARS-CoV(SARSr-CoV)strains generated by the J.Craig Venter Institute in the USA.Moreover,samples from different flu seasons have different SARS-CoV strains,and the divergence between these strains cannot be explained by natural evolution.Our study also shows that retrospective studies using public metagenomic data from past major epidemic outbreaks serve as a genomic strategy for the research of the origins or spread of infectious diseases. | Qi Liu Zhenglin Du Sihui Zhu Wenming Zhao Hua Chen Yongbiao Xue | 2021 | Biosafety and Health2021，3，6： | 0 |
| 14 | Surface passivation and hole extraction:Bifunctional interfacial engineering toward high-performance all-inorganic CsPbIBr2 perovskite solar cells with efficiency exceeding 12%显示文摘All-inorganic CsPbIBr2perovskite solar cells(PSCs)have attracted considerable research attention in recent years due to their excellent thermal stability.However,their power conversion efficiencies(PCEs)are relatively low and still far below the theoretical limit.Here,we report the use of an organic dye molecule(namely VG1-C8)as a bifunctional interlayer between perovskite and the hole-transport layer in CsPbIBr2PSCs.Combined experimental and theoretical calculation results disclose that the multiple Lewis base sites in VG1-C8 can effectively passivate the trap states on the perovskite films.Meanwhile,theπ-conjugated dye molecule significantly accelerates the hole extraction from the perovskite absorber as evidenced by the photoluminescence analysis.Consequently,the VG1-C8 treatment simultaneously boosts the photovoltage and photocurrent density values from 1.26 V and 10.80 mA cm^(-2) to 1.31 V and 12.44 m A cm^(-2),respectively.This leads to a significant enhancement of PCE from 9.20%to12.10%under one sun irradiation(AM 1.5G).To our knowledge,this is the record efficiency reported so far for CsPbIBr_(2) PSCs.Thus,the present work demonstrates an effective interfacial passivation strategy for the development of highly efficient PSCs. | Qi Liu Junming Qiu Xianchang Yan Yuemeng Fei Yue Qiang Qingyan Chang Yi Wei Xiaoliang Zhang Wenming Tian Shengye Jin Ze Yu Licheng Sun | 2022 | Journal of Energy Chemistry2022，31，11： | 0 |
| 15 | Technology and Its Application:MIS with Geographical Inform ation显示文摘Despite the constant expansion of the application of GIS, in recent ye ars, the data processing ability of the application system developed with GIS_To ol is quite limited. Management information system with the geographical informa tion can not only be used to deal with data commonly, but also deal with digitiz ed maps as GIS_Tool does. Thus it can help the user develop an application syste m high qualified both in maps and traditional data. This paper, by taking the method of integrated MapInfo in the application of Vis ual Basic as an example, mainly illustrates two technological problems integrat ed map technology and the multiuserization of MapInfo geographical information. And also the concrete solutions to the problems are provided. | Chen Wenming Qi Huan Graduate School, Huazhong University of Science and Technology, W uhan 430074 | 2003 | Journal of China University of Geosciences2003，14，1： | 0 |
| 16 | Carrier diffusion coefficient is independent of defects in CH_(3)NH_(3)PbBr_(3) single crystals:Direct evidence显示文摘Organolead halide perovskite solar cells have attracted extensive interests in recent years. Thanks to innovations in materials process and technology, the power conversion efficiency (PCE) of perovskites-based solar cells increases from 3.8% to 25.2%[1–4].In evaluating the quality of perovskite materials, a few key photophysical properties such as the lifetime, mobility and diffusion length of photogenerated carriers are usually measured. | Chunyi Zhao Qi Sun Rongrong Cui Jing Leng Wenming Tian Shengye Jin | 2021 | Journal of Energy Chemistry2021，30，7： | 0 |
| 17 | Ongoing Positive Selection Drives the Evolution of SARS-CoV-2 Genomes显示文摘SARS-CoV-2 is a new RNA virus affecting humans and spreads extensively throughout the world since its first outbreak in December,2019.Whether the transmissibility and pathogenicity of SARS-CoV-2 in humans after zoonotic transfer are actively evolving,and driven by adaptation to the new host and environments is still under debate.Understanding the evolutionary mechanism underlying epidemiological and pathological characteristics of COVID-19 is essential for predicting the epidemic trend,and providing guidance for disease control and treatments.Interrogating novel strategies for identifying natural selection using within-species polymorphisms and 3,674,076 SARSCoV-2 genome sequences of 169 countries as of December 30,2021,we demonstrate with population genetic evidence that during the course of SARS-CoV-2 pandemic in humans,1)SARS-CoV-2 genomes are overall conserved under purifying selection,especially for the 14 genes related to viral RNA replication,transcription,and assembly;2)ongoing positive selection is actively driving the evolution of 6 genes(e.g.,S,ORF3a,and N)that play critical roles in molecular processes involving pathogen–host interactions,including viral invasion into and egress from host cells,and viral inhibition and evasion of host immune response,possibly leading to high transmissibility and mild symptom in SARS-CoV-2 evolution.According to an established haplotype phylogenetic relationship of 138 viral clusters,a spatial and temporal landscape of 556 critical mutations is constructed based on their divergence among viral haplotype clusters or repeatedly increase in frequency within at least 2 clusters,of which multiple mutations potentially conferring alterations in viral transmissibility,pathogenicity,and virulence of SARS-CoV-2 are highlighted,warranting attention. | Yali Hou Shilei Zhao Qi Liu Xiaolong Zhang Tong Sha Yankai Su Wenming Zhao Yiming Bao Yongbiao Xue Hua Chen | 2022 | Genomics, Proteomics & Bioinformatics2022，20，6： | 0 |
| 18 | Intrinsically Viscoelastic Supramolecular Conjugated Polymer toward Suppressing Coffee-Ring Effect显示文摘The rational molecular design of light-emitting conjugated polymers that inherently suppress the ubiquitous coffee-ring effect(CRE)is a great challenge and the critical bottleneck for printing displays.Herein,we describe a supramolecular route to construct an intrinsically viscoelastic rigid conjugated polymer(RCP)(PHDPF-Cz)toward avoiding the CRE without sacrificing optoelectronic properties.Theπ-πstacking interactions derived fromthe pendant carbazole(Cz)units enable PHDPF-Cz to self-assemble into criss-cross nanofibers and endow its solutionwith great viscosity.Consequently,a high-quality and continuous PHDPFCz film was obtained by impeding the transport of aggregates to the droplet edge due to outward capillary flow during evaporation,in sharp contrast to the random aggregate migration and rapid precipitation generated fromthe controlled poly[4-(6-(9H-diphenylaniline-9-yl)hexyloxy)-9,9-diphenylfluorene]-co-[5-(6-(9H-diphenylaniline-9-yl)hexyloxy)-9,9-diphenylfluorene]and poly(9,9-dioctylfluorene)solutions.Finally,an efficient random laser is also achieved based on these cross-linked films with ultrastable single-chain excitonic behavior,confirming the effectiveness of our design strategy. | Yamin Han Lubing Bai Xiang An Man Xu Chuanxin Wei Zongqiong Lin Mengna Yu Jinyi Lin Lili Sun Ning Sun Changting Wei Linghai Xie Xuehua Ding Qi Wei Chengrong Yin Chenghui Li Wenming Su Wei Huang | 2022 | CCS Chemistry2022，4，11： | 0 |
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