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| 1 | Biallelic mutations in CDC20 cause female infertility characterized by abnormalities in oocyte maturation and early embryonic development显示文摘Dear Editor,Previously,the Mendelian phe no types in huma n oocyte maturation arrest,fertilization failure and early embryonic arrest,are largely underestimated.In recent years,'missing'Men delian phe no types and genes in these processes are beginning to be uncovered by us and others(Huang et al.,2014;Alazami et al..2015;Feng et al.,2016;Xu et al.,2016;Chen et al.,2017;Sang et al.,2019).However,the genetic basis for majority of patients resulting from abnormalities in these phe no types remains to be elucidated. | Lin Zhao Songguo Xue Zhongyuan Yao Juanzi Shi Biaobang Chen Ling Wu Lihua Sun Yao Xu Zheng Yan Bin Li Xiaoyan Mao Jing Fu Zhihua Zhang Jian Mu Wenjing Wang Jing Du Shuai Liu Jie Dong Weijie Wang Qiaoli Li Lin He Li Jin Xiaozhen Liang Yanping Kuang Xiaoxi Sun Lei Wang Qing Sang | 2020 | Protein & Cell2020，11，12： | 10 |
| 2 | Substrate stiffness modulates bone marrow-derived macrophage polarization through NF-κB signaling pathway显示文摘The stiffness of the extracellular matrix(ECM)plays an important role in regulating the cellular programming.However,the mechanical characteristics of ECM affecting cell differentiation are still under investigated.Herein,we aimed to study the effect of ECM substrate stiffness on macrophage polarization.We prepared polyacrylamide hydrogels with different substrate stiffness,respectively.After the hydrogels were confirmed to have a good biocompatibility,the bone marrow-derived macrophages(BMMs)from mice were incubated on the hydrogels.With simulated by the low substrate stiffness,BMMs displayed an enhanced expression of CD86 on the cell surface and production of reactive oxygen species(ROS)in cells,and secreted more IL-1βand TNF-αin the supernatant.On the contrary,stressed by the medium stiffness,BMMs expressed more CD206,produced less ROS,and secreted more IL-4 and TGF-β.In vivo study by delivered the hydrogels subcutaneously in mice,more CD68+CD86+cells around the hydrogels with the low substrate stiffness were observed while more CD68+CD206+cells near by the middle stiffness hydrogels.In addition,the expressions of NIK,phosphorylated p65(pi-p65)and phosphorylated IκB(pi-IκB)were significantly increased after stimulation with low stiffness in BMMs.Taken together,these findings demonstrated that substrate stiffness could affect macrophages polarization.Low substrate stiffness promoted BMMs to shift to classically activated macrophages(M1)and the middle one to alternatively activated macrophages(M2),through modulating ROS-initiated NF-κB pathway.Therefore,we anticipated ECM-based substrate stiffness with immune modulation would be under consideration in the clinical applications if necessary. | Mimi Chen Yu Zhang Pinghui Zhou Xingzhi Liu Huan Zhao Xichao Zhou Qiaoli Gu Bin Li Xuesong Zhu Qin Shi | 2020 | Bioactive Materials2020，5，4： | 7 |
| 3 | Characterization and immunogenicity of bone marrow-derived mesenchymal stem cells under osteoporotic conditions显示文摘Mesenchymal stem cells(MSCs)are characterized by their multilineage potential and low immunogenicity.However,the properties of MSCs under pathological conditions are unclear.The current study investigated the differentiation potential and immunological characteristics of bone marrow-derived MSCs from ovariectomized-osteoporotic rats(OP-BMSCs).Although the expression of cell morphology-and stemness-related surface markers was similar between OP-BMSCs and BMSCs from healthy rats(H-BMSCs),the proliferation rate was significantly decreased compared with that of H-BMSCs.Regarding multilineage potential,osteogenesis and chondrogenesis abilities of OP-BMSCs decreased,but the adipogenesis ability was significantly enhanced compared with that of H-BMSCs.As expected,decreased osteogenesis following osteogenic induction resulted in reduced expression ofβ-catenin,osteocalcin,and runt-related transcription factor 2 in OP-BMSCs.Remarkably,the expression of the co-stimulatory proteins CD40 and CD80 was significantly higher,whereas the expression of the negative costimulatory molecule programmed cell death ligand 1 was significantly lower in the OP-BMSCs than that in H-BMSCs.Consequently,H-BMSCs inhibited the proliferation and secretion of inflammatory cytokines from anti-CD3 antibody-activated T cells,whereas OP-BMSCs did not.These results indicate that decreased osteogenesis and increased immunogenicity of OPBMSCs contribute to bone loss in osteoporosis. | Yingkang Huang Yin Yin Yanzheng Gu Qiaoli Gu Huilin Yang Zhengyu Zhou Qin Shi | 2020 | Science China(Life Sciences)2020，63，3： | 4 |
| 4 | 18beta-glycyrrhetinic acid induces ROS-mediated apoptosis to ameliorate hepatic fibrosis by targeting PRDX1/2 in activated HSCs显示文摘Hepatic stellate cells(HSCs)are essential drivers of fibrogenesis.Inducing activated-HSC apoptosis is a promising strategy for treating hepatic fibrosis.18beta-glycyrrhetinic acid(18b-GA)is a natural compound that exists widely in herbal medicines,such as Glycyrrhiza uralensis Fisch,which is used for treating multiple liver diseases,especially in Asia.In the present study,we demonstrated that 18b-GA decreased hepatic fibrosis by inducing the apoptosis in activated HSCs.18b-GA inhibited the expression of a-smooth muscle actin and collagen type Ⅰ alpha-1.Using a chemoproteomic approach derived from activity-based protein profiling,together with cellular thermal shift assay and surface plasmon resonance,we found that 18b-GA covalently targeted peroxiredoxin 1(PRDX1)and peroxiredoxin 2(PRDX2)proteins via binding to active cysteine residues and thereby inhibited their enzymatic activities.18b-GA induced the elevation of reactive oxygen species(ROS),resulting in the apoptosis of activated HSCs.PRDX1 knockdown also led to ROS-mediated apoptosis in activated HSCs.Collectively,our findings revealed the target proteins and molecular mechanisms of 18b-GA in ameliorating hepatic fibrosis,highlighting the future development of 18b-GA as a novel therapeutic drug for hepatic fibrosis. | Qian Zhang Piao Luo Liuhai Zheng Jiayun Chen Junzhe Zhang Huan Tang Dandan Liu Xueling He Qiaoli Shi Liwei Gu Jiahao Li Qiuyan Guo Chuanbin Yang Yin Kwan Wong Fei Xia Jigang Wang | 2022 | Journal of Pharmaceutical Analysis2022，12，4： | 3 |
| 5 | Novel SFTSV Phylogeny Reveals New Reassortment Events and Migration Routes显示文摘Severe fever with thrombocytopenia syndrome virus(SFTSV),the causative agent of a febrile human disease,was first identified from central and eastern provinces in China,and later in Japan and South Korea.Hubei Province is one of the major SFTS epidemic areas in the central part of China.This study reported the isolation of 11 new SFTSV strains from patients in Hubei Province collected in 2017.Extensive phylogenetic analyses were conducted based on the complete coding sequences of SFTSV segments including the new strains.It was suggested that five different SFTSV genotypes were circulating in Hubei,and 15 reassortment patterns and migration pathways correlated with each genotype were identified,which was more than previously recognized.Hubei Province was more involved in the evolutionary events of SFTSV than that previously thought in which the evolutionary events of SFTSV were reported to be independent from those in other epidemic regions.Further divergence of SFTSV strains was suggested by pairwise comparison of SFTSV sequences from each genotype and sequence identity normalized to representative strain in genotype C1.Subsequently,amino acid variations specific for genotype(s),strain(s),or cluster(s)were inspected,which may be related to differential biological activity of SFTSV strains/genotypes.In conclusion,we analyzed the current status of SFTSV phylogeny in Hubei Province and discussed the possible events correlated to SFTSV evolution.It provided an in-depth insight into SFTSV evolution,raising concerns for the use of proper SFTSV strains in future studies. | Xiaoli Wu Mingyue Li Yanfang Zhang Boyun Liang Junming Shi Yaohui Fang Zhengyuan Su Mengmeng Li Wenjing Zhang Ling Xu Jun Wang Qiaoli Wu Shuang Tang Hualin Wang Tao Zhang Cheng Peng Xin Zheng Fei Deng Shu Shen | 2021 | Virologica Sinica2021，36，2： | 2 |
| 6 | Discovery and repurposing of artemisinin显示文摘Malaria is an ancient infectious disease that threatens millions of lives globally even today.The discovery of artemisinin,inspired by traditional Chinese medicine(TCM),has brought in a paradigm shift and been recognized as the“best hope for the treatment of malaria”by World Health Organization.With its high potency and low toxicity,the wide use of artemisinin effectively treats the otherwise drug-resistant parasites and helps many countries,including China,to eventually eradicate malaria.Here,we will first review the initial discovery of artemisinin,an extraordinary journey that was in stark contrast with many drugs in western medicine.We will then discuss how artemisinin and its derivatives could be repurposed to treat cancer,inflammation,immunoregulation-related diseases,and COVID-19.Finally,we will discuss the implications of the“artemisinin story”and how that can better guide the development of TCM today.We believe that artemisinin is just a starting point and TCM will play an even bigger role in healthcare in the 21st century. | Qiaoli Shi Fei Xia Qixin Wang Fulong Liao Qiuyan Guo Chengchao Xu Jigang Wang | 2022 | Frontiers of Medicine2022，16，1： | 2 |
| 7 | Wetting and evaporation behaviors of molten Mg on partially oxidized SiC substrates显示文摘 | Dan Zhang Ping Shen Laixin Shi Qiaoli Lin Qichuan Jiang | 2010 | Applied Surface Science2010，，23： | 1 |
| 8 | 大型水库消落带中西来稗种子耐淹性沿高程梯度的种内分化显示文摘在水文节律稳定、每年蓄水时间很长且水位涨落落差很大的水库中,生长在水库库岸消落带不同高程区域中的植物每年要面对长时间的不同强度的水淹,这种每年发生持续的水淹胁迫可能会导致水库库岸消落带中生长的植物的种子耐淹能力发生种内分化。西来稗(Echinochloa crusgalli var.zelayensis)是在三峡水库消落带中自然生长的优势一年生植物,在三峡水库消落带植被及消落带生态环境保护中发挥着重要作用。本研究的目的在于探究三峡水库消落带中生长的西来稗种子的耐淹能力是否已发生种内分化,以及此分化的发生是否与较弱的种子扩散能力有关。在本研究中,采集来自三峡水库消落带不同高程区域中生长的西来稗种子,在三峡水库即将蓄水时将所有采集的种子放置于三峡水库消落带中4个不同高程位置接受不同深度不同时长的水库蓄水水淹,同时把所有采集的种子也放置于不会被水库蓄水淹没的非消落带河岸上作为对照处理;测定种子在水中的漂浮持续时间和蓄水期间水库水位上涨速度以量化在水库蓄水水位上涨时种子的扩散能力,水库蓄水结束水退后检测放置于水库消落带和非消落带河岸上的种子的种子完好率、种子萌发能力和成苗率。研究结果发现,在经历三峡水库蓄水水淹后,西来稗种子的完好率和最终成苗率随种子母株在消落带中所处的高程的增加而显著降低,表明在三峡水库成库运行七年后,在三峡水库消落带中生长的西来稗的种子耐淹能力已发生了种内分化。三峡水库蓄水水位上涨时西来稗种子在水中较短的漂浮持续时间和水库蓄水时较慢的水位上升速度限制了西来稗种子的水媒传播和扩散距离,从而有利于西来稗种子的耐淹能力发生种内分化。 | Songping Liu Feng Lin Xiaolei Su Qiaoli Ayi Hangang Niu Shaohua Shi Binna Wan Bo Zeng Xiaoping Zhang | 2021 | Journal of Plant Ecology2021，14，1： | 0 |
| 9 | A highly efficient protein corona-based proteomic analysis strategy for the discovery of pharmacodynamic biomarkers显示文摘The composition of serum is extremely complex,which complicates the discovery of new pharmacodynamic biomarkers via serum proteome for disease prediction and diagnosis.Recently,nanoparticles have been reported to efficiently reduce the proportion of high-abundance proteins and enrich lowabundance proteins in serum.Here,we synthesized a silica-coated iron oxide nanoparticle and developed a highly efficient and reproducible protein corona(PC)-based proteomic analysis strategy to improve the range of serum proteomic analysis.We identified 1,070 proteins with a median coefficient of variation of 12.56%using PC-based proteomic analysis,which was twice the number of proteins identified by direct digestion.There were also more biological processes enriched with these proteins.We applied this strategy to identify more pharmacodynamic biomarkers on collagen-induced arthritis(CIA)rat model treated with methotrexate(MTX).The bioinformatic results indicated that 485 differentially expressed proteins(DEPs)were found in CIA rats,of which 323 DEPs recovered to near normal levels after treatment with MTX.This strategy can not only help enhance our understanding of the mechanisms of disease and drug action through serum proteomics studies,but also provide more pharmacodynamic biomarkers for disease prediction,diagnosis,and treatment. | Yuqing Meng Jiayun Chen Yanqing Liu Yongping Zhu Yin-Kwan Wong Haining Lyu Qiaoli Shi Fei Xia Liwei Gu Xinwei Zhang Peng Gao Huan Tang Qiuyan Guo Chong Qiu Chengchao Xu Xiao He Junzhe Zhang Jigang Wang | 2022 | Journal of Pharmaceutical Analysis2022，12，6： | 0 |
| 10 | Solvent-dependent evolution of cyclic penta-twinned rhodium icosahedral nanocrystals and their enhanced catalytic properties显示文摘由于他们的唯一的几何、电子的结构答应性质的周期的 penta 孪生的高贵金属 nanocrystals 展览。然而,周期的 penta 孪生的 nanostructures 的控制合成,特别有一个高连贯的精力的高贵金属(例如, Rh ) ,是很困难的,并且相应生长机制充分没被理解。此处,我们报导一个灵巧的一个壶为周期的 penta 孪生的 Rh icosahedral nanocrystals 的合成的热水的途径。它除了调整表面被发现那由改变盖住的代理人的集中或范畴的免费精力,溶剂可能在 Rh 水晶表面上影响表面活化剂的吸附能力,它在表面导致一个变化免费精力并且因此允许 Rh 周期的penta孪生的 nanostructures 的形成。另外由于他们的唯一的电子、几何的结构, Rh icosahedral nanocrystals 作为与单个水晶的 Rh 有四面的 nanocrystals 和商业 Rh 黑色相比为乙醇的 electrooxidation 展出优异催化活动和稳定性。 | Yanan Yang Jiawei Zhang Yajing Wei Qiaoli Chen Zhenming Cao Huiqi Li Jiayu Chen Jueli Shi Zhaoxiong Xie Lansun Zheng | 2018 | Nano Research2018，11，2： | 0 |
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