|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | SIRT6 safeguards human mesenchymal stem cells from oxidative stress by coactivating NRF2显示文摘 | Huize Pan Di Guan Xiaomeng Liu Jingyi Li Lixia Wang Jun Wu Junzhi Zhou Weizhou Zhang Ruotong Ren Weiqi Zhang Ying Li Jiping Yang Ying Hao Tingting Yuan Guohong Yuan Hu Wang Zhenyu Ju Zhiyong Mao Jian Li Jing Qu FuchouTang Guang-Hui Liu | 2016 | Cell Research2016，26，2： | 32 |
| 2 | The influence of the Madden-Julian Oscillation activity anomalies on Yunnan's extreme drought of 2009-2010显示文摘Yunnan Province of China suffered a record-breaking drought that persisted from autumn 2009 into spring 2010. The present study examined the physical causes of this extreme drought event in terms of persistent anomalies of intraseasonal oscillation in the tropical atmosphere (the Madden-Julian Oscillation hereafter the MJO). The results show that the occurrence of severe drought in Yunnan was caused directly by deficient rainfall lasting from the summer of 2009 to the spring of 2010. Further exploration reveals a persistent positive variation of MJO index 1 from June to October. Accordingly, the convective activity over the Bay of Bengal (BOB) weakened continuously, and then an anomalous descending airflow was induced over the tropical Indian Ocean, resulted in the anomalous weakening of vertical Asian monsoon circulation in South Asia. Consequently, the transport of water vapor from the tropical Indian Ocean to Yunnan decreased abnormally, leading to persistent below-normal rainfall over Yunnan from summer to autumn in 2009. As a result, a severe drought began to appear in autumn. In the winter of 2009-2010, MJO index 1 remained persistently positive, indicating the continuous weakening of convective activity over the BOB. The atmospheric circulation associated with the persistent positive anomalies in the MJO also demonstrated anomalous patterns. Specifically, there was an anomalous high-pressure ridge stretching from South Asia through the Tibetan Plateau and into the western part of southwestern China. This indicates that the atmospheric circulation over Yunnan was dominated by vertical descending airflow in the high-pressure ridge. Simultaneously, the India-Burma trough was weakened, which resulted in unfavorable conditions for the transport of water vapor from the BOB to Yunnan, causing the observed persistent deficient precipitation in winter and the subsequently intensified drought. Therefore, the persistent anomalies in MJO activity in the tropical atmosphere played an important role in the occurrence of the extreme drought event in Yunnan in 2009-2010. | LU JunMei JU JianHua REN JuZhang GAN WeiWei | 2012 | Science China Earth Sciences2012，55，1： | 25 |
| 3 | Altered trends in carbon uptake in China’s terrestrial ecosystems under the enhanced summer monsoon and warming hiatus显示文摘The carbon budgets in terrestrial ecosystems in China are strongly coupled with climate changes.Over the past decade,China has experienced dramatic climate changes characterized by enhanced summer monsoon and decelerated warming.However,the changes in the trends of terrestrial net ecosystem production(NEP)in China under climate changes are not well documented.Here,we used three ecosystem models to simulate the spatiotemporal variations in China's NEP during 1982–2010 and quantify the contribution of the strengthened summer monsoon and warming hiatus to the NEP variations in four distinct climatic regions of the country.Our results revealed a decadal-scale shift in NEP from a downtrend of–5.95 Tg C/yr^2(reduced sink)during 1982–2000 to an uptrend of 14.22 Tg C/yr^2(enhanced sink)during 2000–10.This shift was essentially induced by the strengthened summer monsoon,which stimulated carbon uptake,and the warming hiatus,which lessened the decrease in the NEP trend.Compared to the contribution of 56.3%by the climate effect,atmospheric CO2 concentration and nitrogen deposition had relatively small contributions(8.6 and 11.3%,respectively)to the shift.In conclusion,within the context of the global-warming hiatus,the strengthening of the summer monsoon is a critical climate factor that enhances carbon uptake in China due to the asymmetric response of photosynthesis and respiration.Our study not only revealed the shift in ecosystem carbon sequestration in China in recent decades,but also provides some insight for understanding ecosystem carbon dynamics in other monsoonal areas. | Honglin He Shaoqiang Wang Li Zhang Junbang Wang Xiaoli Ren Lei Zhou Shilong Piao Hao Yan Weimin Ju Fengxue Gu Shiyong Yu Yuanhe Yang Miaomiao Wang Zhongen Niu Rcmg Ge Huimin Yan Mei Huang Guoyi Zhou Yongfei Bai Zongqiang Xie Zhiyao Tang Bingfang Wu Leiming Zhang Nianpeng He Qiufeng Wang Guirui Yu | 2019 | National Science Review2019，6，3： | 24 |
| 4 | Copper-chelating therapeutic effect in Wilson disease with different clinical phenotypes and polymorphisms of ATP7B gene显示文摘CopperchelatingtherapeuticefectinWilsondiseasewithdiferentclinicalphenotypesandpolymorphismsofATP7BgeneRENMingShan1,HUWen?.. | REN MingShan1, HU WenBin1, ZHANG Zhi1, JU ShuangWu1, FAN YuXin2, WANG GongQiang1 and YANG RenMin1Keywords Wilson disease/therapy copper chelating agents ATP7B gene mutations | 1998 | World Journal of Gastroenterology1998，4，4： | 15 |
| 5 | Study on protecting effects of Baicalin and Octreotide on hepatic injury in rats with severe acute pancreatitis显示文摘AIM: To investigate the protective effects and mechanisms of Baicalin and Octreotide on hepatic injury in rats with severe acute pancreatitis (SAP). METHODS: The SAP rat models were prepared and randomly assigned to the model control group, Baicalin treated group, and Octreotide treated group while other healthy rats were assigned to the sham-operated group. Rat mortality, levels of ALT, AST, liver and pancreas pathological changes in all groups were observed at 3, 6 and 12 h after operation. Tissue microarray (TMA) sections of hepatic tissue were prepared to observe expression levels of Bax, Bcl-2 protein and Caspase-3, and changes of apoptotic indexes.RESULTS: Rat survival at 12 h, expression levels of Bax, Caspase-3 protein and apoptotic indexes of liver were all significantly higher in treated groups than in model control group. While the liver and pancreas pathological scores, contents of ALT, AST, and expression levels of Bcl-2 protein were all lower in treated groups than in the model control group. CONCLUSION: Both Baicalin and Octreotide can protect rats with SAP by decreasing the contents of ALT, AST and expression levels of Bcl-2 protein, and improving the expression levels of Bax protein, Caspase-3 protein, and inducing apoptosis. | Xi-Ping Zhang Guang-Hua Feng Wei Zhu Yang Cai Qi-Jun Yang Tong-Fa Ju Qi xie Wen-Qin Yuan Jie Zhang Zheng Ren | 2008 | World Journal of Gastroenterology2008，14，42： | 13 |
| 6 | Chemical screen identifies a geroprotective role of quercetin in premature aging显示文摘Aging increases the risk of various diseases. The main goal of aging research is to find therapies that attenuate aging and alleviate aging-related diseases. In this study, we screened a natural product library for geroprotective compounds using Werner syndrome (WS) human mesenchymal stem cells (hMSCs), a premature aging model that we recently established. Ten candidate compounds were identified and quercetin was investigated in detail due to its leading effects. Mechanistic studies revealed that quercetin alleviated senescence via the enhancement of cell proliferation and restoration of heterochromatin architecture in WS hMSCs. RNA-sequencing analysis revealed the transcriptional commonalities and differences in the geroprotective effects by quercetin and Vitamin C. Besides WS hMSCs, quercetin also attenuated cellular senescence in Hutchinson-Gilford progeria syndrome (HGPS) and physiological-aging hMSCs. Taken together, our study identifies quercetin as a geroprotective agent against accelerated and natural aging in hMSCs, providing a potential therapeutic intervention for treating age-associated disorders. | Lingling Geng Zunpeng Liu Weiqi Zhang Wei Li Zeming Wu Wei Wang Ruotong Ren Yao Su Peichang Wang Liang Sun Zhenyu Ju Piu Chan Moshi Song Jing Qu Guang-Hui Liu | 2019 | Protein & Cell2019，10，6： | 13 |
| 7 | First dark matter search results from the PandaX-Ⅰ experiment显示文摘We report on the first dark-matter(DM)search results from PandaX-I,a low threshold dual-phase xenon experiment operating at the China JinPing Underground Laboratory.In the 37-kg liquid xenon target with 17.4 live-days of exposure,no DM particle candidate event was found.This result sets a stringent limit for low-mass DM particles and disfavors the interpretation of previously-reported positive experimental results.The minimum upper limit,3.7×10-44cm2,for the spin-independent isoscalar DM-particle-nucleon scattering cross section is obtained at a DM-particle mass of 49 GeV/c2at 90%confidence level. | XIAO MengJiao XIAO Xiang ZHAO Li CAO XiGuang CHEN Xun CHEN YunHua CUI XiangYi FANG DeQing FU ChangBo GIBONI Karl L. GONG HaoWei GUO GuoDong HU Jie HUANG XingTao JI XiangDong JU YongLin LEI SiAo LI ShaoLi LIN Qing LIU HuaXuan LIU JiangLai LIU Xiang LORENZON Wolfgang MA YuGang MAO YaJun NI KaiXuan PUSHKIN Kirill REN XiangXiang SCHUBNELL Michael SHEN ManBing STEPHENSON Scott TAN AnDi TARL Greg WANG HongWei WANG JiMin WANG Meng WANG XuMing WANG Zhou WEI YueHuan WU ShiYong XIE PengWei YOU YingHui ZENG XiongHui ZHANG Hua ZHANG Tao ZHU ZhongHua | 2014 | Science China(Physics,Mechanics & Astronomy)2014，57，11： | 10 |
| 8 | PandaX: a liquid xenon dark matter experiment at CJPL显示文摘PandaX is a large liquid-xenon detector experiment usable for direct dark-matter detection and 136Xe double-beta decay search.The central vessel was designed to accommodate a staged target volume increase from initially 120 kg(stage I)to 0.5 t(stage II)and eventually to a multi-ton scale.The experiment is located in the Jinping Deep-Underground Laboratory in Sichuan,China.The detector operates in dual-phase mode,allowing detection of both prompt scintillation,and ionization charge through proportional scintillation.In this paper a detailed description of the stage I detector design and performance as well as results established during the commissioning phase are presented. | CAO XiGuang CHEN Xun CHEN YunHua CUI XiangYi FANG DeQing FU ChangBo GIBONI Karl L. GONG HaoWei GUO GuoDong HE Ming HU Jie HUANG XingTao JI XiangDong JU YongLin LI ShaoLi LIN Qing LIU HuaXuan LIU JiangLai LIU Xiang LORENZON Wolfgang MA YuGang MAO YaJun NI KaiXuan PUSHKIN Kirill REN XiangXiang SCHUBNELL Michael SHEN ManBing SHI YuJie STEPHENSON Scott TAN AnDi TARLé Greg WANG HongWei WANG JiMing WANG Meng WANG XuMing WANG Zhou WEI YueHuan WU ShiYong XIAO MengJiao XIAO Xiang XIE PengWei YE Tao YOU YingHui ZEN XiongHui ZHANG Hua ZHANG Tao ZHAO HaiYing ZHAO Li ZHOU XiaoPeng ZHU ZhongHua | 2014 | Science China(Physics,Mechanics & Astronomy)2014，57，8： | 9 |
| 9 | A Survey on Emerging Computing Paradigms for Big Data显示文摘The explosive growth of data volume and the ever-increasing demands of data value extraction have driven us into the era of big data. The '5V'(Variety,Velocity, Volume, Value, and Veracity) characteristics of big data pose great challenges to traditional computing paradigms and motivate the emergence of new solutions.Cloud computing is one of the representative technologies that can perform massive-scale and complex data computing by taking advantages of virtualized resources, parallel processing and data service integration with scalable data storage. However, as we are also experiencing the revolution of Internet-of-things(IoT), the limitations of cloud computing on supporting lightweight end devices significantly impede the flourish of cloud computing at the intersection of big data and IoT era. It also promotes the urgency of proposing new computing paradigms. We provide an overview on the topic of big data, and a comprehensive survey on how cloud computing as well as its related technologies can address the challenges arisen by big data.Then, we analyze the disadvantages of cloud computing when big data encounters IoT, and introduce two promising computing paradigms, including fog computing and transparent computing, to support the big data services of IoT. Finally, some open challenges and future directions are summarized to foster continued research efforts into this evolving field of study. | ZHANG Yaoxue REN Ju LIU Jiagang XU Chugui GUO Hui LIU Yaping | 2017 | Chinese Journal of Electronics2017，26，1： | 8 |
| 10 | Posterior chamber phakic intraocular lens implantation for high myopia显示文摘AIM:To evaluate the efficacy,safety and stability of posterior chamber phakic intraocular lens implantation for the correction of high myopia.METHODS:Retrospective case review of 82 eyes(43patients)undergoing implantable Coliamer lens(ICL)placement by a single surgeon(Xiao-Wei Gao)to correct preoperative mean spherical equivalents between-9.00diopter(D)and-23.00D.Main outcome measures included uncorrected visual acuity(UCVA),refraction,best spectacle-corrected visual acuity(BSCVA),endothelial cell density(ECD),intraocular pressure(IOP),lens transparency,postoperative uveitis.Visante anterior segment optical coherence tomography(AS-OCT)was used to measure anterior chamber depth(ACD)and the position of ICL.RESULTS:Mean follow-up was 6.54±3.26 months(range 3-12 months).Predictability of the manifest spherical equivalent(SE)refraction to within±1.OOD was achieved in 88%of eyes and±0.50D in 72.5%of eyes.The mean postoperative manifest SE refraction was-1.85±0.72D,with 96.34%of eyes maintaining or gaining≥1 line(s)of BSCVA.The mean 3-month postoperative ECD decreased but had no statistically difference compared with the preoperative ECD.Of the 7 eyes(8.54%)with a mild transient increase in intraocular pressure(up to 30mmHg),none required a second surgical procedure or prolonged topical medication.There was no loss of lens transparency.Pigmented precipitates were observed in 5 eyes(6.09%).The mean preoperative ACD measured with AS-OCT was 3.28±0.14mm,three months after surgery,the mean ACD was2.45±0.22mm.Anterior chamber depth showed astatistically significant reduction.One eye(1.22%)had ICL spontaneous rotation,81 eyes(98.78%)of the lens remained correctly centered.CONCLUSION:The implantation of ICL is an effective surgical option for the management of high myopia.But its long time effect and safety still need more time to prove. | Yan Ju Xiao-Wei Gao Bing Ren | 2013 | International Journal of Ophthalmology(English edition)2013，6，6： | 7 |
| 11 | Geochronology and Geochemistry of Metallogenetic Porphyry Bodies from the Nongping Au-Cu Deposit in the Eastern Yanbian Area, NE China: Implications for Metallogenic Environment显示文摘The metallogenetic porphyry bodies in the Nongping Au-Cu deposit, in the eastern Yanbian area, mainly include porphyritic granodiorite and biotite granodiorite porphyry. They are featured with high silicon and enrichment in sodium, and classified into sodic rocks of low-K tholeiitic basalt series. Except slightly low Sr content, the rock basically has the geochemical characteristics of the adakite: relatively high A12O3 content, relatively low MgO content, depletion in Y and Yb; relative enrichment in large ion lithophile elements (LILEs) and light rare-earth elements (LREEs), relatively low content of high field strength elements (HFSEs); positive Eu anomaly or weak negative Eu anomaly. In situ zircon dating technology LA-MC-ICP-MS was used to conduct single-grain zircon dating of biotite granodiorite porphyry, and the results show that the age of metallogenetic porphyry body is 100.04±0.88 Ma, indicating that the porphyry bodies were emplaced in the late Cretaceous period. According to the regional tectonic setting and the comparison with the same kind of deposits, we think that the metallogenetic porphyry bodies in the Nongping Au-Cu deposit have a close genetic connection with the subduction of the Pacific plate in the late Yanshanian period. The adakitic magma generated from partial melting of the subducting plate has high formation temperature, high oxygen fugacity, and volatile constituents' enrichment, so it is helpful for enrichment of metallogenetic elements and plays an important role in the formation of porphyry Au-Cu deposits in this region. | REN Yunsheng JU Nan ZHAO Hualei WANG Hui HOU Kejun LIU Shen | 2012 | Acta Geologica Sinica(English Edition)2012，86，3： | 7 |
| 12 | New innovations in pavement materials and engineering:A review on pavement engineering research 2021显示文摘Sustainable and resilient pavement infrastructure is critical for current economic and environmental challenges.In the past 10 years,the pavement infrastructure strongly supports the rapid development of the global social economy.New theories,new methods,new technologies and new materials related to pavement engineering are emerging.Deterioration of pavement infrastructure is a typical multi-physics problem.Because of actual coupled behaviors of traffic and environmental conditions,predictions of pavement service life become more and more complicated and require a deep knowledge of pavement material analysis.In order to summarize the current and determine the future research of pavement engineering,Journal of Traffic and Transportation Engineering(English Edition)has launched a review paper on the topic of'New innovations in pavement materials and engineering:A review on pavement engineering research 2021'.Based on the joint-effort of 43 scholars from 24 well-known universities in highway engineering,this review paper systematically analyzes the research status and future development direction of 5 major fields of pavement engineering in the world.The content includes asphalt binder performance and modeling,mixture performance and modeling of pavement materials,multi-scale mechanics,green and sustainable pavement,and intelligent pavement.Overall,this review paper is able to provide references and insights for researchers and engineers in the field of pavement engineering. | JTTE Editorial Office Jiaqi Chen Hancheng Dan Yongjie Ding Yangming Gao Meng Guo Shuaicheng Guo Bingye Han Bin Hong Yue Hou Chichun Hu Jing Hu Ju Huyan Jiwang Jiang Wei Jiang Cheng Li Pengfei Liu Yu Liu Zhuangzhuang Liu Guoyang Lu Jian Ouyang Xin Qu Dongya Ren Chao Wang Chaohui Wang Dawei Wang Di Wang Hainian Wang Haopeng Wang Yue Xiao Chao Xing Huining Xu Yu Yan Xu Yang Lingyun You Zhanping You Bin Yu Huayang Yu Huanan Yu Henglong Zhang Jizhe Zhang Changhong Zhou Changjun Zhou Xingyi Zhu | 2021 | Journal of Traffic and Transportation Engineering(English Edition)2021，8，6： | 7 |
| 13 | Simulating the exchanges of carbon dioxide, water vapor and heat over Changbai Mountains temperate broad- leaved Korean pine mixed forest ecosystem显示文摘A process-based ecosystem productivity model BEPS (Boreal Ecosystem Productivity Simulator) was updated to simulate half-hourly exchanges of carbon, water and energy between the atmosphere and terrestrial ecosystem at a temperate broad-leaved Korean pine forest in the Changbai Mountains, China. The BEPSh model is able to capture the diurnal and seasonal variability in carbon dioxide, water vapor and heat fluxes at this site in the growing season of 2003. The model validation showed that the simulated net ecosystem productivity (NEP), latent heat flux (LE), sensible heat flux (Hs) are in good agreement with eddy covariance measurements with an R2 value of 0.68, 0.86 and 0.72 for NEP, LE and Hs, respectively. The simulated annual NEP of this forest in 2003 was 300.5 gC/m2, and was very close to the observed value. Driving this model with different climate scenarios, we found that the NEP in the Changbai Mountains temperate broad-leaved Korean pine mixed forest ecosystem was sensitive to climate variability, and the current carbon sink will be weakened under the condition of global warming. Furthermore, as a process-based model, BEPSh was also sensitive to physiological parameters of plant, such as maximum Rubisco activity (Vcmax) and the maximum stomatal conductance (gmax), and needs to be carefully calibrated for other applications. | WANG Qiufeng, NIU Dong, YU Guirui, REN Chuanyou, WEN Xuefa, CHEN Jingming & JU Weimin Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China Bureau of Science and Technology for Resources and Environment, Chinese Academy of Sciences, Beijing 100864, China Department of Geography, University of Toronto, Toronto M5S 3G3, Canada Graduate School of the Chinese Academy of Sciences, Beijing 100039, China Shenyang Agricultural University, Shenyang 100161, China | 2005 | Science China Earth Sciences2005，48，z1： | 6 |
| 14 | Dark matter direct search sensitivity of the PandaX-4T experiment显示文摘The Panda X-4T experiment, a 4-ton scale dark matter direct detection experiment, is being planned at the China Jinping Underground Laboratory. In this paper we present a simulation study of the expected background in this experiment. In a 2.8-ton fiducial mass and the signal region between 1-10 keV electron equivalent energy, the total electron recoil background is found to be 4.9 × 10^(-5) kg^(-1) d^(-1) keV^(-1). The nuclear recoil background in the same region is 2.8 × 10^(-7) kg^(-1) d^(-1) keV^(-1). With an exposure of 5.6 ton-years, the sensitivity of Panda X-4 T could reach a minimum spin-independent dark matter-nucleon cross section of 6 × 10^(-48) cm^2 at a dark matter mass of 40 Ge V/c^2. | HongGuang Zhang Abdusalam Abdukerim Wei Chen Xun Chen YunHua Chen XiangYi Cui BinBin Dong DeQing Fang ChangBo Fu Karl Giboni Franco Giuliani LinHui Gu XuYuan Guo ZhiFan Guo Ke Han ChangDa He ShengMing He Di Huang XingTao Huang Zhou Huang Peng Ji XiangDong Ji YongLin Ju ShaoLi Li Yao Li Heng Lin HuaXuan Liu JiangLai Liu YuGang Ma YaJun Mao KaiXiang Ni JinHua Ning XiangXiang Ren Fang Shi AnDi Tan AnQing Wang Cheng Wang HongWei Wang Meng Wang QiuHong Wang SiGuang Wang XiuLi Wang XuMing Wang Zhou Wang MengMeng Wu ShiYong Wu JingKai Xia MengJiao Xiao PengWei Xie BinBin Yan JiJun Yang Yong Yang ChunXu Yu JuMin Yuan JianFeng Yue Dan Zhang Tao Zhang Li Zhao QiBin Zheng JiFang Zhou Ning Zhou XiaoPeng Zhou | 2019 | Science China(Physics,Mechanics & Astronomy)2019，62，3： | 6 |
| 15 | Synthesis and biological evaluation of novel triazole derivatives as antifungal agents显示文摘A series of 1-(benzylamino)-2-(2,4-difluorophenyl)-3-(1H-1,2,4-triazol-l-yl)propan-2-ols compounds were synthesized and evaluated for their antifungal activities in vitro.The results showed that compounds 6A and 6B exhibited good antifungal activity.Compound 6A8 showed the strongest antifungal activity,which was significantly higher than that of the lead compounds and positive-control drugs Fluconazole and Itraconazole.In particular,the antifungal activity of compound 6A8 against Candida albicans and Candida krusei(MIC80 both at 0.00097μg/mL) was 515 and 64 times that of Fluconazole,respectively.The structure-activity relationships of the synthesized compounds were discussed,and the docking model of the target compounds with fungal lanosterol 14α-demethylase (CYP51) was analyzed. | Hui Tang Can-Hui Zheng Xiao-Hui Ren Jia Liu Na Liu Jia-Guo Lv Ju Zhu You-Jun Zhou | 2013 | Chinese Chemical Letters2013，24，3： | 6 |
| 16 | m6A promotes R-loop formation to facilitate transcription termination显示文摘R-loops are three-stranded nucleic acid structures consisting of a DNA:RNA heteroduplex and a displaced single DNA strand.They constitute a prevalent genomic feature with important functions in all kingdoms of eukaryotes.1,2,3 For example,R-loops have been found over unmethylated CpG island promoters,G-rich terminators,and many other regulatory chromosomal loci.2,3 Moreover,they are essential for diverse cellular processes,such as immunoglobulin class switching,mitotic chromosome segregation,4 DNA replication and repair,genome stability regulation,5 as well as in multiple steps during transcription.6,7 R-loops can be formed both in cis and in trans;cis R-loops are formed between a nascent RNA and its DNA template following transcribing RNA polymerases and exert co-transcriptional regulatory effects.3,8 Therefore,it is important to control their formation and resolution,and many external factors have already been identified for this process.7 However,it is still unknown whether the intrinsic properties of the RNA itself,such as various modifications,can modulate the formation of DNA:RNA hybrids. | Xin Yang Qian-Lan Liu Wei Xu Yi-Chang Zhang Ying Yang Lin-Fang Ju Jing Chen Yu-Sheng Chen Kuan Li Jie Ren Qianwen Sun Yun-Gui Yang | 2019 | Cell Research2019，29，12： | 5 |
| 17 | Comparison of viral RNA-host protein interactomes across pathogenic RNA viruses informs rapid antiviral drug discovery for SARS-CoV-2显示文摘In contrast to the extensive research about viral protein–host protein interactions that has revealed major insights about how RNA viruses engage with host cells during infection,few studies have examined interactions between host factors and viral RNAs(vRNAs).Here,we profiled vRNA–host protein interactomes for three RNA virus pathogens(SARS-CoV-2,Zika,and Ebola viruses)using ChIRP-MS.Comparative interactome analyses discovered both common and virus-specific host responses and vRNA-associated proteins that variously promote or restrict viral infection.In particular,SARS-CoV-2 binds and hijacks the host factor IGF2BP1 to stabilize vRNA and augment viral translation.Our interactome-informed drug repurposing efforts identified several FDA-approved drugs(e.g.,Cepharanthine)as broad-spectrum antivirals in cells and hACE2 transgenic mice.A co-treatment comprising Cepharanthine and Trifluoperazine was highly potent against the newly emerged SARS-CoV-2 B.1.351 variant.Thus,our study illustrates the scientific and medical discovery utility of adopting a comparative vRNA-host protein interactome perspective. | Shaojun Zhang Wenze Huang Lili Ren Xiaohui Ju Mingli Gong Jian Rao Lei Sun Pan Li Qiang Ding Jianwei Wang Qiangfeng Cliff Zhang | 2022 | Cell Research2022，32，1： | 5 |
| 18 | Deprivation/Reperfusion-induced Apoptosis by Inhibiting Endoplasmic Reticulum Stress and Autophagy in PC12 Cells显示文摘This study aimed to elucidate the molecular mechanisms by which berberine protects against cerebral ischemia/reperfusion(I/R)injury.The oxygen-glucose deprivation/reperfusion(OGD/R)PC12 model was established.Cell counting kit-8(CCK-8)was used to detect the toxicity of berberine and the viability of PC12 cells.Hoechst 33258 staining and flow cytometry were used to observe the nuclear morphology,and changes of apoptosis and reactive oxygen species(ROS),respectively.Western blotting and immunofluorescence assay were employed to detect autophagy-related proteins[microtubule-associated protein 1A/1B-light chain 3(LC3),P62/SQSTM-1,Beclin-1]and endoplasmic reticulum(ER)stress-related markers[glucose-regulated protein 78(GRP78),C/EBP homologous protein(CHOP),Bcl-2-associated X(Bax)and cleaved caspase-3].The GFP-RFP-LC3 adenovirus was used to assay the change of autophagic flux.Our results showed that berberine could increase the viability of PC12 cells,decrease the concentrations of ROS after OGD/R treatment,and suppress OGD/R-induced ER stress and autophagy.Moreover,the results revealed the involvement of the mammalian target of rapamycin(mTOR)pathway in the induction of autophagy,and berberine could activate the phosphorylation of mTOR and thus mitigate autophagy.In conclusion,our study suggested that berberine may protect against OGD/R-induced apoptosis by regulating ER stress and autophagy,and it holds promises in the treatment of cerebral I/R injury. | Peng XIE Zhen-kui REN Ju LV Yu-Mei HU Zhi-zhong GUAN Wen-feng YU | 2020 | Current Medical Science2020，40，6： | 4 |
| 19 | Visualization of aging-associated chromatin alterations with an engineered TALE system显示文摘在实时房间的特定的 genomic loci 的可视化在多样的生物过程期间是为在染色质建筑学的动态变化的调查的一个前提,例如细胞的老化。然而,当前的精确 genomic 成像方法被缺乏妨碍荧光灯有高特性和 signal-to-noise 的探针形成对照。我们发现那常规抄写像使活跃之物的受动器(故事) 趋于形成蛋白质总数,从而在成像应用程序损害他们的性能。通过屏蔽,我们发现有故事的那熔化 thioredoxin 阻止了总数形成,开基于故事的 genomic 成像的完整的力量。用熔化 thioredoxin 的故事(TTALE ) ,我们在各种各样的 genomic loci 完成了高质量的成像并且观察联系老化(epi ) 在在人和老鼠早衰模型的 telomeres 和着丝点的 genomic 改变。重要地,我们鉴别 ribosomal DNA 的摩擦为人的老化作为一个分子的标记重复。我们的学习为精确在 vitro 并且在 vivo 监视染色质动力学建立一个简单、柔韧的成像方法。 | Ruotong Ren Liping Deng Yanhong Xue Keiichiro Suzuki Weiqi Zhang Yang Yu Jun Wu Liang Sun Xiaojun Gong Huiqin Luan Fan Yang Zhenyu Ju Xiaoqing Ren Si Wang Hong Tang Lingling Geng Weizhou Zhang Jian Li Jie Qiao Tao Xu Jing Qu Guang-Hui Liu | 2017 | Cell Research2017，27，4： | 4 |
| 20 | A novel intronic circular RNA,circGNG7,inhibits head and neck squamous cell carcinoma progression by blocking the phosphorylation of heat shock protein 27 at Ser78 and Ser82显示文摘Background:There is increasing evidence that circular RNAs(circRNAs)play a significant role in pathological processes including tumorigenesis.In contrast to exonic circRNAs,which are the most frequently reported circRNAs in cancer so far,the studies of intronic circRNAs have been greatly lagged behind.Here,we aimed to investigate the regulatory role of intronic circRNAs in head and neck squamous cell carcinoma(HNSCC).Methods:We conducted whole-transcriptome sequencing with four pairs of primary tumor tissues and adjacent normal tissues from HNSCC patients.Then,we characterized circGNG7 expression in HNSCC tissues and cell lines and explored its association with the prognosis of HNSCC patients.We also identified interactions between circGNG7 and functional proteins,which alter downstream signaling that regulate HNSCC progression.Results:In this study,we identified a new intronic circRNA,circGNG7,and validated its functional roles in HNSCC progression.CircGNG7 was predominately localized to the cytoplasm,and its expression was downregulated in both HNSCC tissues andCAL27,CAL33,SCC4,SCC9,HN6,and HN30 cells.Low expression of circGNG7 was significantly correlated with poor prognosis in HNSCC patients.Consistent with this finding,overexpression of circGNG7 strongly inhibited tumor cell proliferation,colony formation,in vitro migration,and in vivo tumor growth.Mechanistically,the expression of circGNG7 in HNSCC cells was regulated by the transcription factor SMAD family member 4(SMAD4).Importantly,we discovered that circGNG7 could bind to serine residues 78 and 82 of the functional heat shock protein 27(HSP27),occupying its phosphorylation sites and hindering its phosphorylation,which reduced HSP27-JNK/P38 mitogen-activated protein kinase(MAPK)oncogenic signaling.Downregulation of circGNG7 expression in HNSCC increased HSP27-JNK/P38 MAPK signaling and promoted tumor progression.Conclusions:Our results revealed that a new intronic circRNA,circGNG7,functions as a strong tumor suppressor and that circGNG7/HSP27-JNK/P38 MAPK signaling is a novel mechanism by which HNSCC progression can be controlled. | Houyu Ju Zhenrong Hu Dongliang Wei Jinyun Huang Xinyi Zhang Mengyu Rui Zhi Li Xiaomeng Zhang Jingzhou Hu Wei Guo Guoxin Ren | 2021 | Cancer Communications2021，41，11： | 4 |
您所在的机构暂未开通此部分的功能，请联系管理员