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| 1 | Nitrogen-doped fluorescence carbon dots as multi-mechanism detection for iodide and curcumin in biological and food samples显示文摘Iodine ion is one of the most indispensable anions in living organisms,particularly being an important substance for the synthesis of thyroid hormones.Curcumin is a yellow-orange polyphenol compound derived from the rhizome of Curcuma longa L.,which has been commonly used as a spice and natural coloring agent,food additives,cosmetics as well as Chinese medicine.However,excess curcumin may cause DNA inactivation,lead to a decrease in intracellular ATP levels,and trigger the tissue necrosis.Therefore,quantitative detection of iodine and curcumin is of great significance in the fields of food and life sciences.Herein,we develop nitrogen-doped fluorescent carbon dots(NCDs)as a multi-mechanism detection for iodide and curcumin in actual complex biological and food samples,which was prepared by a one-step solid-phase synthesis using tartaric acid and urea as precursors without adding any other reagents.An assembled NCDs-Hg^(2+) fluorescence-enhanced sensor for the quantitative detection of I^(-) was established based on a fluorescence“turn-off-on”mechanism in a linear range of 0.3-15μM with a detection limit of 69.4 nM and successfully quantified trace amounts of I^(-) in water samples and urine sample.Meanwhile,the as-synthesized NCDs also can be used as a fluorescent quenched sensor for curcumin detection based on the synergistic internal filtration effect(IFE)and static quenching,achieving a good linear range of 0.1-20μM with a satisfactory detection limit of 29.8 nM.These results indicate that carbon dots are potential sensing materials for iodine and curcumin detection for the good of our health. | Xiaodan Tang Hongmei Yu Brian Bui Lingyun Wang Christina Xing Shaoyan Wang Mingli Chen Zhizhi Hu Wei Chen | 2021 | Bioactive Materials2021，6，6： | 11 |
| 2 | Ecological principle meets cancer treatment: treating children with acute myeloid leukemia with low-dose chemotherapy显示文摘Standard chemotherapy regimens for remission induction of pediatric acute myeloid leukemia(AML) are associated with significant morbidity and mortality. We performed a cohort study to determine the impact of reducing the intensity of remission induction chemotherapy on the outcomes of selected children with AML treated with a low-dose induction regimen plus granulocyte colony stimulating factor(G-CSF)(low-dose chemotherapy(LDC)/G-CSF). Complete response(CR) after two induction courses was attained in 87.0%(40/46) of patients receiving LDC/G-CSF. Post-remission therapy was offered to all patients, and included standard consolidation and/or stem cell transplantation. During the study period, an additional 94 consecutive children with AML treated with standard chemotherapy(SDC) for induction(80/94(85.1%) of the patients attained CR after induction Ⅱ, P = 0.953) and post-remission. In this non-randomized study, there were no significant differences in 4-year event-free(67.4 vs. 70.7%;P = 0.99)and overall(70.3 vs. 74.6%, P = 0.69) survival in the LDC/G-CSF and SDC cohorts, respectively. After the first course of induction, recovery of white blood cell(WBC) and platelet counts were significantly faster in patients receiving LDC/G-CSF than in those receiving SDC(11.5 vs. 18.5 d for WBCs(P < 0.001);15.5 vs. 22.0 d for platelets(P < 0.001)). To examine the quality of molecular response, targeted deep sequencing was performed. Of 137 mutations detected at diagnosis in 20 children who attained hematological CR after two courses of LDC/G-CSF(n = 9) or SDC(n = 11), all of the mutations were below the reference value(variant allelic frequency <2.5%) after two courses, irrespective of the treatment group. In conclusion, children with AML receiving LDC/G-CSF appear to have similar outcomes and mutation clearance levels, but significantly lower toxicity than those receiving SDC. Thus, LDC/G-CSF should be further evaluated as an effective alternative to remission induction in pediatric AML. | Yixin Hu Aili Chen Xinchang Zheng Jun Lu Hailong He Jin Yang Ya Zhang Pinpin Sui Jingyi Yang Fuhong He Yi Wang Peifang Xiao Xin Liu Yinmei Zhou Deqing Pei Cheng Cheng Raul C. Ribeiro Shaoyan Hu Qian-fei Wang | 2019 | National Science Review2019，6，3： | 3 |
| 3 | Hydride vapor phase epitaxy for gallium nitride substrate显示文摘Due to the remarkable growth rate compared to another growth methods for gallium nitride(GaN)growth,hydride vapor phase epitaxy(HVPE)is now the only method for mass product GaN substrates.In this review,commercial HVPE systems and the GaN crystals grown by them are demonstrated.This article also illustrates some innovative attempts to develop homebuilt HVPE systems.Finally,the prospects for the further development of HVPE for GaN crystal growth in the future are also discussed. | Jun Hu Hongyuan Wei Shaoyan Yang Chengming Li Huijie Li Xianglin Liu Lianshan Wang Zhanguo Wang | 2019 | Journal of Semiconductors2019，40，10： | 3 |
| 4 | The priming induction regimen of HAG as a low dose chemotherapy strategy in AML clonal evolution显示文摘Chemotherapy employs chemical substances to interfere with the growth of cancer cells,and is a major treatment strategy in human cancer including acute myeloid leukemia(AML).Although they often effectively kill fast-dividing tumor cells,chemotherapeutic drugs also profoundly | CHEN AiLi YANG JingYi HU ShaoYan WANG Qian-Fei | 2015 | Science China(Life Sciences)2015，58，12： | 2 |
| 5 | A web-based vir- tual laboratory on a frequency modulation experimen显示文摘 | Chi Chung Ko Chen B M Shaoyan Hu | 2001 | IEEE Transactions on Systems Man and Cybernetics2001，31，3： | 1 |
| 6 | Linifanib (ABT-869) Enhances Radiosensitivity of Head and Neck Squamous Cell Carcinoma Cells显示文摘 | Heng-Wei Hsu Daila S. Gridley Paul D. Kim Shaoyan Hu Rosalia de Necochea-Campion Robert L. Ferris Chien-Shing Chen Saied Mirshahidi | 2013 | Oral Oncology2013，，： | 1 |
| 7 | Tumor heterogeneity of acute myeloid leukemia: insights from single-cell sequencing显示文摘Individual tumors comprise genetically and epigenetically heterogeneous subclones,each of which is presumably associated with a distinct function,such as self-renewal or drug sensitivity.The dissection of such intratumoral heterogeneity is crucial to understand how tumors evolve during disease progression and under the selection of therapeutic intervention.As a paradigm of cancer intratumoral heterogeneity and clonal evolution,acute myeloid leukemia(AML)has been shown to possess complex clonal architecture based on karyotype studies,as well as deep sequencing of mixed cellular populations using next-generation sequencing(NGS)technologies.The recent development of single-cell sequencing(SCS)methods provides a powerful tool to allow analysis of genomes,transcriptomes,proteomes,and epigenomes at an individual cell level.The technologies applied in AML have broadened our understanding of AML heterogeneity and provided new insights for the development of novel therapeutic strategies.In this review,we summarize the progress in the research of AML heterogeneity using SCS technology and discuss the limitations and future direction regarding how SCS can contribute to AML prognosis and treatment. | AiLi Chen ShaoYan Hu Qian-Fei Wang | 2019 | Blood Science2019，1，1： | 0 |
| 8 | Response of T cells in vivo Induced by Repeated Superantigen Treatments at Different Time Intervals显示文摘我们在 vivo 调查了 T 房间的反应到 staphylococcal 肠毒素 A (海) 注射。我们发现有 10 亩陷阱的最佳的剂量的海的单个注射显著地弄皱 CD4 和 CD8 的表示。在在注射之间的海重新注射和时间间隔强烈影响了 CD4^+ 和 CD8^+T 房间的应答的海角以后,有在 vivo 的海反应的 Tcells 的扩大。T 房间的变应力缺乏在三 SEAtreatments 以后被观察。在注射之间的时间间隔主要影响了 CD4^+ Tcells,不是 CD8^+ T 房间的无答复的海角。CD4^+ T 房间的变应力缺乏跟随的显著删除在短间隔被导致,并且没有 CD4^+ T 房间的明显的删除的变应力缺乏在长间隔被导致。Wealso 发现 anergic 状态在 vivo 是可逆的。重复的海刺激带了白介素(IL ) 的 todown 规定 IL-10 的 -2, 和高水平。这研究证明两个 CD4^+and CD8^+ 告知海的 T 房间对海应答在 vivo,和第三注射重新质问被需要导致 T 房间的变应力缺乏。 | Yang HUANG Yanfang SUI Xiumin ZHANG Shaoyan SI Wei GE Peizhen HU Xia LI Bin MA | 2007 | Acta Biochimica et Biophysica Sinica2007，39，7： | 0 |
| 9 | Venous thromboembolism in children with acute lymphoblastic leukemia in China:a report from the Chinese Children’s Cancer Group-ALL-2015显示文摘Venous thromboembolism(VTE)is a complication in children with acute lymphoblastic leukemia(ALL).The Chinese Children’s Cancer Group-ALL-2015 protocol was carried out in China,and epidemiology,clinical characteristics,and risk factors associated with VTE were analyzed.We collected data on VTE in a multiinstitutional clinical study of 7640 patients with ALL diagnosed in 20 hospitals from January 2015 to December 2019.First,VTE occurred in 159(2.08%)patients,including 90(56.6%)during induction therapy and 108(67.92%)in the upper extremities.T-ALL had a 1.74-fold increased risk of VTE(95%CI 1.08–2.8,P=0.022).Septicemia,as an adverse event of ALL treatment,can significantly promote the occurrence of VTE(P<0.001).Catheter-related thrombosis(CRT)accounted for 75.47%(n=120);and,symptomatic VTE,58.49%(n=93),which was more common in patients aged 12–18 years(P=0.023),non-CRT patients(P<0.001),or patients with cerebral thrombosis(P<0.001).Of the patients with VTE treated with anticoagulation therapy(n=147),4.08%(n=6)had bleeding.The VTE recurrence rate was 5.03%(n=8).Patients with VTE treated by non-ultrasoundguided venous cannulation(P=0.02),with residual thrombus(P=0.006),or with short anticoagulation period(P=0.026)had high recurrence rates.Thus,preventing repeated venous puncture and appropriately prolonged anticoagulation time can reduce the risk of VTE recurrence. | Mengmeng Yin Hongsheng Wang Xianmin Guan Ju Gao Minghua Yang Ningling Wang Tianfeng Liu Jingyan Tang Alex WK Leung Fen Zhou Xuedong Wu Jie Huang Hong Li Shaoyan Hu Xin Tian Hua Jiang Jiaoyang Cai Xiaowen Zhai Shuhong Shen Qun Hu | 2023 | Frontiers of Medicine2023，17，3： | 0 |
| 10 | Common Postzygotic Mutational Signatures in Healthy Adult Tissues Related to Embryonic Hypoxia显示文摘Postzygotic mutations are acquired in normal tissues throughout an individual’s lifetime and hold clues for identifying mutagenic factors.Here,we investigated postzygotic mutation spectra of healthy individuals using optimized ultra-deep exome sequencing of the time-series samples from the same volunteer as well as the samples from different individuals.In blood,sperm,and muscle cells,we resolved three common types of mutational signatures.Signatures A and B represent clocklike mutational processes,and the polymorphisms of epigenetic regulation genes influence the proportion of signature B in mutation profiles.Notably,signature C,characterized by C>T transitions at GpCpN sites,tends to be a feature of diverse normal tissues.Mutations of this type are likely to occur early during embryonic development,supported by their relatively high allelic frequencies,presence in multiple tissues,and decrease in occurrence with age.Almost none of the public datasets for tumors feature this signature,except for 19.6%of samples of clear cell renal cell carcinoma with increased activation of the hypoxia-inducible factor 1(HIF-1)signaling pathway.Moreover,the accumulation of signature C in the mutation profile was accelerated in a human embryonic stem cell line with drug-induced activation of HIF-1α.Thus,embryonic hypoxia may explain this novel signature across multiple normal tissues.Our study suggests that hypoxic condition in an early stage of embryonic development is a crucial factor inducing C>T transitions at GpCpN sites;and individuals’genetic background may also influence their postzygotic mutation profiles. | Yaqiang Hong Dake Zhang Xiangtian Zhou Aili Chen Amir Abliz Jian Bai Liang Wang Qingtao Hu Kenan Gong Xiaonan Guan Mengfei Liu Xinchang Zheng Shujuan Lai Hongzhu Qu Fuxin Zhao Shuang Hao Zhen Wu Hong Cai Shaoyan Hu Yue Ma Junting Zhang Yang Ke Qian-Fei Wang Wei Chen Changqing Zeng | 2022 | Genomics, Proteomics & Bioinformatics2022，20，1： | 0 |
| 11 | RNA interference affects tumorigenicity and expression of insulin-like growth factor-1,insulin-like growth factor-1 receptor,and basic fibroblast growth factor-2 in rat C6 glioma cells显示文摘BACKGROUND:Human gliomas are more likely to express basic fibroblast growth factor-2(FGF-2), insulin-like growth factor-1(IGF-1),and IGF-1 receptor(IGF-1R) than normal brain tissue.These factors activate signal transduction systems of Ras/MAPK and PI3K/Akt,which promote glioma growth. OBJECTIVE:To utilize RNA interference(RNAi) technique to down-regulate FGF-2,IGF-1,and IGF-1R gene expression,and to investigate the effects of these genes on rat C6 glioma cells,as well as the feasibility of RNAi for treating glioma. DESIGN,TIME AND SETTING:This neurooncological,randomized,controlled,in vivo and in vitro experiment,which used RNAi methodology,was performed at the Laboratory of Molecular Biology, Institute of Biochemistry,Chinese Academy of Sciences between August 2005 and February 2008. MATERIALS:Rat C6 cell lines were purchased from Shanghai Institute of Cellular Biology Affiliated to Chinese Academy of Sciences.Small interfering RNA(siRNA) was synthesized by Shanghai GenePharma.Anti-IGF-1,anti-IGF-1R,anti-FGF-2,anti-mouse and anti-rabbit IgG G1-HRP antibodies were provided by Santa Cruz Biotechnology,USA.Four to six week-old BALB/c nude mice were purchased from the Laboratory Animal Center,Chinese Academy of Sciences. METHODS:C6 glioma cells were transfected with siRNA,which was chemically synthesized in vitro to correspond to endogenous FGF-2,IGF-1,and IGF-1R genes.The inhibition ratio of targeting mRNA expression was detected by semiquantitative RT-PCR,and protein expression was determined by Western blot analysis.C6 glioma cell proliferation was observed using a growth curve. C6 glioma cell apoptosis rate and cell cycle were detected by flow cytometry.C6 glioma cell growth regression was observed by transwell migration assay.In addition,nude mouse subcutaneous tumor models were used in this study.For studying the anti-tumor effects of IGF-1 and IGF-1R siRNA,two blank control groups,with six mice each,were set up:A(2.5μg siRNA was injected one week after C6 cells were inoculated,i.e.,when tumor volume reached 8 mm×8 mm) and B(siRNA was injected at the same time with C6 cells were inoculated.To study the effects of FGF-2 siRNA, the groups consisted of a blank control group,negative control group,2.6μg siRNA group,4μg siRNA group,and 5.3μg siRNA group,with six mice each. MAIN OUTCOME MEASURES:mRNA and protein inhibition ratio of FGF-2,IGF-1,and IGF-1R;C6 glioma cell proliferation,apoptosis,and cycle growth arrest;C6 glioma cell growth regression and subcutaneous tumorigenicity rates. RESULTS:All siRNA constructs proved to be effective.After 48 hours,transfection of 200 nmol/L siRNA resulted in a FGF-2 or IGF-1R gene inhibition ratio>80%and an IGF-1 gene inhibition ratio of approximately 70%.Protein expression levels for FGF-2,IGF-1,and IGF-1R decreased in a dose-dependent manner following siRNA transfection,with an inhibition rate>85%,60%,and 50%, respectively.C6 glioma cell proliferation and apoptosis rates increased in proportion to siRNA.The apoptosis rate of C6 glioma cells induced by FGF-2,IGF-1,and IGF-1R siRNA was 39.96%,15.07%, and 22.47%,respectively(P<0.01).Transfection of 200 nmol/L IGF or IGF-1R siRNA for 48 hours suppressed C6 glioma cell migration.At 30 days after intratumoral injection of 2.6,4,and 5.3μg FGF-2 siRNA,tumor growth regression rate of FGF-2 siRNA was 56%,67%,and 86%,respectively. The tumor growth regression rate was 71.88%and 45.71%,respectively,when IGF-1 or IGF-1R siRNA was intratumorally injected 1 week after C6 glioma cell transplantation.When IGF-1 or IGF-1R siRNA was intratumorally injected during C6 glioma cell transplantation,the tumor growth regression rate was 78.13%and 74.29%,respectively. CONCLUSION:siRNA transfection downregulated gene expression of FGF-2,IGF-1,and IGF-1R. In addition,siRNA treatment markedly suppressed glioma cell proliferation,growth,and migration, and concomitantly reduced subcutaneous tumorigenicity. | Wanli Dong Jin Hu Shaoyan Hu Yuanyuan Wang Juean Jiang Youxin Jin | 2009 | Neural Regeneration Research2009，4，8： | 0 |
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