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| 1 | A novel human mAb (MERS-GD27) provides prophylactic and postexposure efficacy in MERS-CoV susceptible mice显示文摘Dear Editor,Since September 2012, the Middle East respiratory syndrome coronavirus (MERS-CoV) cases have been reported in more than 27 countries, and more than 2,000 cases have been confirmed in the laboratory (http://www.who.int/emergencies/mers-cov/en/). MERS-CoV causes an acute and severe respiratory illness with a high mortality rate(~35%) in humans (Shi et al., 2017, Zaki et al., 2012).Neutralizing antibodies targeting the spike of MERS-CoV have been shown to be a therapeutic option for treatment of lethal disease (Agrawal et al., 2016, Ying et al., 2014). | Peihua Niu Guangyu Zhao Yao Deng Shihui Sun Wenling Wang Yusen Zhou Wenjie Tan | 2018 | Science China(Life Sciences)2018，61，10： | 11 |
| 2 | Microscopic inspection and tracking of single upconversion nanoparticles in living cells显示文摘Nanoparticles have become new tools for cell biology imaging1,sub-cellular sensing2,super-resolution imaging3,4 and drug delivery5.Long-term 3D tracking of nanoparticles and their intracellular motions have advanced the understanding of endocytosis and exocytosis as well as of active transport processes6–8.The sophisticated operation of correlative optical-electron microscopy9,10 and scientific-grade cameras is often used to study intercellular processes.Nonetheless,most of these studies are still limited by the insufficient sensitivity for separating a single nanoparticle from a cluster of nanoparticles or their aggregates8,11,12.Here we report that our eyes can track a single fluorescent nanoparticle that emits over 4000 photons per 100 milliseconds under a simple microscope setup.By tracking a single nanoparticle with high temporal,spectral and spatial resolution,we show the measurement of the local viscosity of the intracellular environment.Moreover,beyond the colour domain and 3D position,we introduce excitation power density as the fifth dimension for our eyes to simultaneously discriminate multiple sets of single nanoparticles. | Fan Wang Shihui Wen Hao He Baoming Wang Zhiguang Zhou Olga Shimoni Dayong Jin | 2018 | Light(Science & Applications)2018，7，1： | 9 |
| 3 | Rational development of a human antibody cocktail that deploys multiple functions to confer Pan-SARS-CoVs protection显示文摘Structural principles underlying the composition and synergistic mechanisms of protective monoclonal antibody cocktails are poorly defined.Here,we exploited antibody cooperativity to develop a therapeutic antibody cocktail against SARS-CoV-2.On the basis of our previously identified humanized cross-neutralizing antibody H014,we systematically an a lyzed a fully human n aive antibody library and rationally identified a potent neutralizing antibody partner,P17,which confers effective protection in animal model.Cryo-EM studies dissected the nature of the P17 epitope,which is SARS-CoV-2 specific and distinctly different from that of HOI4.High-resolution structure of the SARS-CoV-2 spike in complex with HOM and P17,together with functional investigations revealed that in a two-antibody cocktail,synergistic neutralization was achieved by S1 shielding and conformational locking,thereby blocking receptor attachment and viral membrane fusion,conferring high potency as well as robustness against viral mutation escape.Furthermore,cluster analysis identified a hypothetical 3rd antibody partner for further reinforcing the cocktail as pan-SARS-CoVs therapeutics. | Hangping Yao Yao Sun Yong-Qiang Deng Nan Wang Yongcong Tan Na-Na Zhang Xiao-Feng Li Chao Kong Yan-Peng Xuc Qi Chen Tian-Shu Cao Hui Zhao Xintian Yan Lei Cao Zhe Lv Dandan Zhu Rui Feng Nanping Wu Wenhai Zhang Yuhao Hu Keda Chen Rong-Rong Zhang Qingyu Lv Shihui Sun Yunhua Zhou Run Yan Guan Yang Xinglu Chanjuan Liu Xiangyun Lu Linfang Cheng Hongying Qiu Xing-Yao Huang Tianhao Weng Danrong Shi Weidong Jiang Junbin Shao Lei Wang Jie Zhang Tao Jiang Guojun Lang Cheng-Feng Qinc Lanjuan Li Xiangxi Wang | 2021 | Cell Research2021，31，1： | 5 |
| 4 | Evaluation of seed production in a first-generation seed orchard of Chinese pine (Pinus tabuliformis)显示文摘We evaluated seed production in a first-generation orchard of Chinese pine(Pinus tabuliformis) during the crucial transition period from first generation to advanced generations.Clones varied significantly in all traits related to seed production.Repeatability of these traits ranged from 0 to 0.96.Seed production per ramet(SPPR),seed producing index(SPI),the number of relative female strobili(RFS),the number of scales,and the number of ineffective scales had comparatively high repeatability at 0.86,0.87,0.89,0.96,and 0.91,respectively.Correlation analysis showed that SPPR was greatly influenced by RFS and by the number of full seeds per cone.Finally,we showed that SPI was the best predictor of the seed-producing ability of clones.Our findings will assist seed orchard managers in effectively predicting and improving seed production of Chinese pine seed orchards. | Huwei Yuan Shihui Niu Xianqing Zhou Qingping Du Yue Li Wei Li | 2016 | Journal of Forestry Research2016，27，5： | 4 |
| 5 | Carbon performance evaluation model from the perspective of circular economy~The case of Chinese thermal power enterprise显示文摘Carbon emission reduction is the only way to alleviate environmental problems,such as global warming.Effective evaluation of carbon performance can help enterprises to carry out energy saving and emission reduction activities to a certain extent and promote sustainable development.This paper constructs a carbon performance evaluation index system that includes the four dimensions of carbon resource(energy)input,cycle,output,and carbon management by incorporating the principles of circular economy and the theory of resource value circulation from the perspective of the flow trajectory of carbon-containing resources in the circulation of enterprises combined with the production characteristics of thermoelectric enterprises.Subsequently,combined with the case study,this paper discusses the scientific and practical nature of the system and provides another way of thinking for carbon performance evaluation of micro-enterprises in other industries.This paper expands the application boundary of matter-element model and supplements the literature of carbon performance,which has certain theoretical and practical significance. | Zhifang ZHOU Jinhao LIU Huixiang ZENG Mengmeng XU Shihui LI | 2022 | Frontiers of Engineering Management2022，9，2： | 4 |
| 6 | Single-cell transcriptomic landscape of human blood cells显示文摘High throughput single-cell RNA-seq has been successfully implemented to dissect the cellular and molecular features underlying hematopoiesis.However,an elaborate and comprehensive transcriptome reference of the whole blood system is lacking.Here,we profiled the transcriptomes of 7551 human blood cells representing 32 immunophenotypic cell types,including hematopoietic stem cells,progenitors and mature blood cells derived from 21 healthy donors.With high sequencing depth and coverage,we constructed a single-cell transcriptional atlas of blood cells(ABC) on the basis of both protein-coding genes and long noncoding RNAs(lncRNAs),and showed a high consistence between them.Notably,putative lncRNAs and transcription factors regulating hematopoietic cell differentiation were identified.While common transcription factor regulatory networks were activated in neutrophils and monocytes,lymphoid cells dramatically changed their regulatory networks during differentiation.Furthermore,we showed a subset of nucleated erythrocytes actively expressing immune signals,suggesting the existence of erythroid precursors with immune functions.Finally,a web portal offering transcriptome browsing and blood cell type prediction has been established.Thus,our work provides a transcriptional map of human blood cells at single-cell resolution,thereby offering a comprehensive reference for the exploration of physiological and pathological hematopoiesis. | Xiaowei Xie Mengyao Liu Yawen Zhang Bingrui Wang Caiying Zhu Chenchen Wang Qing Li Yingying Huo Jiaojiao Guo Changlu Xu Linping Hu Aiming Pang Shihui Ma Una Wang Wenbin Cao Shulian Chen Qiuling Li Sudong Zhang Xueying Zhao Wen Zhou Hongbo Luo Guoguang Zheng Erlie Jiang Sizhou Feng Lixiang Chen Lihong Shi Hui Cheng Sha Hao Ping Zhu Tao Cheng | 2021 | National Science Review2021，8，3： | 3 |
| 7 | Late Mesozoic rifting and its deep dynamic mechanisms in the central Sulu orogenic belt: Records from Lingshan Island显示文摘The Lingshan Island scientific drill confirms that two episodes(Laiyang period and Qingshan period) of rifting developed in the central Sulu orogenic belt(SOB) in Late Mesozoic. With a set of methods including fieldwork, drilling, core logging, zircon U-Pb dating and whole rock geochemistry applied, the age, the depositional sequence and the deep dynamic mechanisms of rift evolution were unraveled. The stratigraphic sequence of the Laiyang-Qingshan Groups on Lingshan Island was composed of two different rifting sequences:(1) Laiyang Group(147–125 Ma), which consists of deep-water gravity flow deposits with interlayers of intermediate volcanic rocks;and(2) Lower Qingshan Group(125–119 Ma), which unconformably overlies the former sequence and contains subaerial volcanic deposits and terrestrial deposits. The tectonic environment changed during the evolution of these two episodes of rifting: the rift was in a NNW-SSE extensional environment in the Laiyang period and showed the typical passive rifting character that “lithospheric extension and rifting preceded volcanism”. The passive rifting period was ended by a short WNW-ESE compression at about 125 Ma. After that, the tectonic environment transferred to a strong NW-SE extensional environment and the rifting evolved into a volcanic arc basin in the Qingshan period. The igneous rocks are shoshonitic to high-K calc-alkaline trachyandesites to trachytes with a few intercalated lamprophyres and a rhyolite.The geochemical characteristics of the igneous rocks indicate that they are mantle-derived melts with a metasomatized mantle source and/or crustal contamination. In addition, an increased thinning of the lithosphere happened during the rifting episodes.The low-angle subduction of the Paleo-Pacific plate in the Jurassic weakened the thickened SOB lithospheric mantle. The rollback of the subducting plate started in late Jurassic to early Cretaceous, and the SOB lithospheric mantle was delaminated synchronously because of the gravity collapse. Thus, this caused passive rifting in the Laiyang period. Thereafter, the rollback and trench retreat of the high-angle subducting Paleo-Pacific plate would have achieved its climax, resulting in the strong regional extension. Passive rifting was ended by the crustal uplift caused by asthenospheric upwelling beneath the rift. The lower crust was heated by the upwelling asthenosphere and partially melted to form felsic melts, which were emplaced upwards and erupted explosively. The rift evolved into a volcanic arc basin in the Qingshan period and showed some characteristics of active rifting. Above all, a passive rifting in the Laiyang period and a volcanic arc basin in the Qingshan period developed successively in the Lingshan Island area(the central SOB). This records the transfer of the study area from the Paleo-Tethys tectonic domain to the circum-Pacific tectonic domain. The delamination of SOB lithospheric mantle and the upwelling of asthenospheric material were the deep dynamic mechanisms driving the development and evolution of two rift episodes. Additionally, the rift development was controlled remotely by the subduction of the Paleo-Pacific plate. | Tengfei ZHOU Yaoqi ZHOU Nina SФAGER Paul Martin HOLM Zhenkai ZHANG Jun WANG Zhao LIANG Hongyu MU Yanjun CHENG Feifei LIU Miao WANG Yue ZHANG Hui ZHANG Yangjian GU Shihui DONG Hanjie ZHAO Manjie LI Yang CHEN Yanzi LIU | 2022 | Science China Earth Sciences2022，65，9： | 3 |
| 8 | NK cells play a significant role in immunosurveillance at the early stage of MLL-AF9 acute myeloid leukemia via CD226/CD155 interactions显示文摘Acute myeloid leukemia(AML) is an aggressive hematological malignancy, and the mechanism underlying immune system involvement in leukemia development is unclear. In the present study, we utilized a myeloid/lymphoid or mixed-lineage leukemia; translocated to, 3(MLLT3/MLL-AF9)-induced AML mouse model with or without exposure to irradiation. We found that the leukemia cells could survive and expand in hosts with intact immune systems, whereas leukemia progression was accelerated in mice with impaired immune systems. Moreover, the leukemia cells escaped from host immunosurveillance via editing their immunogenicity, including the up-regulation of an inhibitory antigen(i.e., CD47) and the down-regulation of active antigens(i.e., CD86, CD54, retinoic acid early transcript(RAE), histocompatibility 2, D region locus b(H2-Db) and H2-Dd). Natural killer(NK) cells were activated in the early phase of AML progression, whereas T cells were stimulated in the late phase. Furthermore, NK cell depletion showed that NK cells were necessary for the elimination of leukemia cells in our AML mouse model. Notably, CD155/CD226 primarily mediated the interaction between NK cells and leukemia cells and contributed to the antitumor effects of NK cells during the early phase of AML. Clinical data from patients with diverse hematological malignancies showed that CD155 expression was decreased in hematological malignancies. Taken together, our results demonstrate that NK cells play a pivotal role in immunosurveillance against leukemia cells during the early stage of AML primarily through the CD226/CD155 interaction; however, NK cells are not sufficient to eliminate leukemia cells. | WANG YaJie CHEN Chen DONG Fang MA ShiHui XU Jing GONG YueMin CHENG Hui ZHOU Yuan CHENG Tao HAO Sha | 2015 | Science China(Life Sciences)2015，58，12： | 3 |
| 9 | A consistency improving method in the analytic hierarchy process based on directed circuit analysis显示文摘Test of consistency is critical for the analytic hierarchy process(AHP) methodology. When a pairwise comparison matrix(PCM) fails the consistency test, the decision maker(DM) needs to make revisions. The state of the art focuses on changing a single entry or creating a new matrix based on the original inconsistent matrix so that the modified matrix can satisfy the consistency requirement. However, we have noticed that the reason that causes inconsistency is not only numerical inconsistency, but also logical inconsistency, which may play a more important role in the whole inconsistency. Therefore, to realize satisfactory consistency, first of all, we should change some entries that form a directed circuit to make the matrix logically consistent, and then adjust other entries within acceptable deviations to make the matrix numerically consistent while preserving most of the original comparison information. In this paper, we firstly present some definitions and theories, based on which two effective methods are provided to identify directed circuits. Four optimization models are proposed to adjust the original inconsistent matrix. Finally, illustrative examples and comparison studies show the effectiveness and feasibility of our method. | WU Shihui LIU Xiaodong LI Zhengxin ZHOU Yu | 2019 | Journal of Systems Engineering and Electronics2019，30，6： | 2 |
| 10 | Molecular Cloning,Bioinformatics Analysis and Transcriptional Expression of Virulence-related Gene(exsA)of Vibrio alginolyticus显示文摘[Objectives]The purpose was to investigate the function and mechanism of exsA gene in type III secretion system of Vibrio alginolyticus.[Methods]The full length of exsA was cloned using molecular biology techniques to analyze its biological information.Fluorescence quantitative PCR technology was used to analyze the expression of exsA after different media stress.[Results]The exsA gene contains an open reading frame(ORF)of 861 bp,encoding 286 amino acids.The physico-chemical analysis shows that the molecular structural formula is C1442H2267N393O441S12,the theoretical molecular weight is 32.549 kD,the theoretical pI value is 6.0,and the protein is non-hydrophilic and unstable.The gene does not contain a transmembrane region,and there is no obvious signal peptide.The prediction result of protein subcellular localization shows that the protein is inside the cell.The deduced amino acid sequence and constructed phylogenetic tree show that V.alginolyticus has a close relationship with Vibrio antiquarius.The qPCR results show that the expression level of exsA in different media is different,highest in TSB medium containing bile salts,followed by DMEM medium,and lowest in ordinary TSB medium.[Conclusions]The gene sequence,molecular structure and isoelectric point of exsA,as well as its expression in three different media were obtained. | Weijie ZHANG Liangchuan CHEN Lin LIN Shihui ZHOU Zihao HE Yanqiu LIANG Huanying PANG | 2021 | Asian Agricultural Research2021，13，1： | 2 |
| 11 | Strength-increase mechanism and microstructural characteristics of a biotreated geomaterial显示文摘Microbially induced calcite precipitation(MICP)is a recently proposed method that is environmentally friendly and has considerable potential applications in artificial biotreated geomaterials.New artificial biotreated geomaterials are produced based on the MICP technology for different parent soils.The purpose of this study is to explore the strength-increase mechanism and microstmctural characteristics of the biotreated geomaterial through a series of experiments.The results show that longer mineralization time results in higher-strength biotreated geomaterial.The strength growth rate rapidly increases in the beginning and remains stable afterwards.The calcium ion content significantly increases with the extended mineralization time.When standard sand was used as a parent soil,the calcium ion content increased to a factor of 39 after 7 days.The bacterial cells with attached calcium ions serve as the nucleus of crystallization and fill the pore space.When fine sand was used as a parent soil,the calcium ion content increased to only a factor of 7 after 7 days of mineralization.The nucleus of crystallization could not normally grow because of the limited pore space.The porosity and variation in porosity are clearly affected by the parent soil.Therefore,the strength of the biotreated geomaterial is affected by the parent soil properties,mineralization time,and granular material pore space.This paper provides a basis for theory and experiments for biotreated geomaterials in future engineering practice. | Chi LI Siriguleng BAI Tuanjie ZHOU Hanlong LIU Xiao QIN Shihui LIU Xiaoying LIU Yang XIAO | 2020 | Frontiers of Structural and Civil Engineering2020，14，3： | 2 |
| 12 | The key environmental factors driving the succession of phytoplankton functional groups in Hongfeng Reservoir, southwest China显示文摘Reservoirs are an important water source in many densely populated areas in southwest China.Phytoplankton play an essential role in maintaining the structure and function of reservoir ecosystems.Understanding the succession in phytoplankton communities and the factors driving it are essential for eff ective water quality management in drinking water reservoirs.In this study,water samples were collected monthly at the surface layers from March 2016 to December 2019 in Hongfeng Reservoir,southwest China.The relationship between functional group succession was analyzed based on nonmetric multidimensional scaling analysis(NMDS),redundancy analysis(RDA),succession rate,and other analysis methods.The results showed distinct shifts in the community structure of phytoplankton functional groups within study period.The Cyclotella sp.was dominant in 2016 and 2017,and Pseudanabaena limnetica was the dominant group in 2018 and 2019.It appears that the phytoplankton composition and biomass are closely related to the water temperature and nutrient status in this reservoir.The results clearly showed that the permanganate index(COD_(Mn))was the key factor of dramatic phytoplankton functional group succession,and the change in succession rates was closely caused by total nitrogen concentration(TN).Therefore,the succession pattern and key factors of Hongfeng Reservoir revealed in this study were important guidance for the management of drinking water reservoirs in southwest China.A reasonable limit on exogenous nutrient input should be a priority,especially in high water temperature period. | Libin HAN Qiuhua LI Wensheng CHEN Xing WANG Shihui ZHOU Mengshu HAN Anton BRANCELJ | 2022 | Journal of Oceanology and Limnology2022，40，4： | 2 |
| 13 | Grain size dependence of coercivity in magnetic metal-insulator nanogranular films with u- niaxial magnetic anisotropy 显示文摘 | Yao Dongsheng Ge Shihui Zhou Xueyun Zuo Huaping | 2010 | Journal of Applied Physics2010，107，07： | 1 |
| 14 | A natural compound induced cardiogenic differentiation of endogenous MSCs for repair of infarcted heart显示文摘 | Xiaoli Lin Peng Peng Lei Cheng Shihui Chen Karen Li Zhong Yu Li Ye Hua Mo Zhen Zhou Ming Li | 2011 | Differentiation2011，，1： | 1 |
| 15 | Study on microstructure and soft magnetism of ( Fe65 Co35 ) x ( SiO2 ) l - x nano-granular films with very high ferromagnetic resonance frequency显示文摘 | Dongsheng Yao Shihui Ge Xueyun Zhou | 2010 | Physica B : Condensed Matter2010，405，13： | 1 |
| 16 | Study on the effect of EDTA on the photocatalytic reduction of mercury onto nanoerystalline titania using quartz crystal microbalanee and differential pulse voltammetry 显示文摘 | Zhou Jianhong Deng Chunyan Si Shihui | 2011 | Eleetroehimiea Acta2011，56，5： | 1 |
| 17 | Multiple exosome RNA analysis methods for lung cancer diagnosis through integrated on-chip microfluidic system显示文摘Exosomes are now raising focus as a prospective biomarker for cancer diagnostics and prognosis owing to its unique bio-origin and composition.Exosomes take part in cellular communication and receptor mediation and transfer their cargos(e.g.,proteins,m RNA and DNA).Quantitative analysis of tumor-related nucleic acid mutations can be a potential method to cancer diagnosis and prognosis in early stages.Here we present an integrated microfluidic system for exosome on-chip isolation and lung cancer RNA analysis through droplet digital PCR(dd PCR).Gradient dilution experiments show great linearity over a large concentration range with R^(2)=0.9998.Utilizing the system,four cell lines and two mutation targets were parallelly detected for mutation analysis.The experiments demonstrated mutation heterogeneity and the results were agree with cell researches.These results proved our integrated microfluidic system as a promising means for early cancer diagnosis and prognosis in the era of liquid biopsy. | Yunxing Lu Zhaoduo Tong Zhenhua Wu Xiaoyu Jian Lin Zhou Shihui Qiu Chuanjie Shen Hao Yin Hongju Mao | 2022 | Chinese Chemical Letters2022，33，6： | 1 |
| 18 | Knockdown of Burton’s tyrosine kinase confers potent protection against sepsis-induced acute lung injury显示文摘 | Panyu Zhou Bing Ma Shuogui Xu Shijie Zhang Hongtai Tang Shihui Zhu Shichu Xiao Daofeng Ben Zhaofan Xia | 2014 | Cell Biochemistry and Biophysics2014，，2： | 1 |
| 19 | Comparisonof 7-irradiation with other pretreatments processingfollowed with simultaneous saccharification and fer-mentation on bioconversion of microcrystalline cellu-lose for bioethanol production显示文摘 | Liu Yun Zhou Hua Wang Shihui | 2015 | Bioresour Technol2015，182，： | 1 |
| 20 | Breeding High-Grain Quality and Blast Resistant Rice Variety Using Combination of Traditional Breeding and Marker-Assisted Selection显示文摘We reported a novel rice variety developed by integrating the advantages of high eating quality from Xiangwanxiaa 17(XWX17)and rice blast resistance from Gumei 4(GM4)using a combination of traditional breeding and marker-assisted selection(MAS).Using molecular markers for QTLs/genes responsible for amylose content(AC),fragrance and blast resistance,three inbred lines were selected. | Zhou Zheng Sun Liang Hu Wenbin Zhou Bin TAo Shuhua Zhang Shihui LU Yanmei Zhao Zhenghong Chen Caiyan | 2021 | Rice science2021，28，5： | 1 |
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