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| 1 | Identification of biomarkers for hepatocellular carcinoma by semiquantitative immunocytochemistry显示文摘AIM:To investigate the expression of key biomarkers in hepatoma cell lines,tumor cells from patients’blood samples,and tumor tissues.METHODS:We performed the biomarker tests in two steps.First,cells plated on coverslips were used to assess biomarkers,and fluorescence intensities were calculated using the NIH Image J software.The measured values were analyzed using the SPSS19.0 software to make comparisons among eight cell lines.Second,eighty-four individual samples were used to assess the biomarkers’expression.Negative enrichment of the blood samples was performed,and karyocytes were isolated and dropped onto pretreated glass slides for further analysis by immunofluorescence staining.Fluorescence intensities were compared among hepatocellular carcinoma(HCC)patients,chronic HBV-infected patients,and healthy controls following methods similar to those used for cell lines.The relationships between the expression of biomarkers and clinical pathological parameters were analyzed by Spearman rank correlation tests.In addition,we studied the distinct biomarkers’expression with three-dimensional laser confocal microscopy reconstructions,and Kaplan-Meier survival analysis was performed to understand the clinical significance of these biomarkers.RESULTS:Microscopic examination and fluorescence intensity calculations indicated that cytokeratin 8/18/19(CK)expression was significantly higher in six of the seven HCC cell lines examined than in the control cells,and the expression levels of asialoglycoprotein receptor(ASGPR)and glypican-3(GPC3)were higher in all seven HCC cell lines than in the control.Cells obtained from HCC patients’blood samples also displayed significantly higher expression levels of ASGPR,GPC3,and CK than cells from chronic HBV-infected patients or healthy controls;these proteins may be valuable surface biomarkers for identifying HCC circulating tumor cells isolated and enriched from the blood samples.The stem cell-like and epithelial-mesenchymal transition-related biomarkers could be detected on the karyocyte slides.ASGPR and GPC3 were expressed at high levels,and thus three-dimensional reconstructions were used to observe their expression in detail.This analysis indicated that GPC3 was localized in the cytoplasm and membrane,but that ASGPR had a polar localization.Survival analyses showed that expression of GPC3 and ASGPR is associated with a patient’s overall survival(OS).CONCLUSION:ASGPR,GPC3,and CK may be valuable HCC biomarkers for CTC detection;the expression of ASGPR and GPC3 might be helpful for understanding patients’OS. | Hong Mu Kai-Xuan Lin Hong Zhao Shu Xing Cong Li Fang Liu Hai-Zhen Lu Ze Zhang Yu-Lin Sun Xi-Yun Yan Jian-Qiang Cai Xiao-Hang Zhao | 2014 | World Journal of Gastroenterology2014，20，19： | 8 |
| 2 | A prospective study of vertical transmission of hepatitis C virus显示文摘AprospectivestudyofverticaltransmissionofhepatitisCvirusSUNDeGui1,LIUCaiYun1,MENGZongDa2,SUNYongDe2,WANGShuCong1,YANGYu... | SUN De Gui 1, LIU Cai Yun 1, MENG Zong Da 2, SUN Yong De 2, WANG Shu Cong 1, YANG Yu Qi 1, LIANG Zheng Lun 3 and ZHUANG Hui 3 | 1997 | World Journal of Gastroenterology1997，3，2： | 8 |
| 3 | Acute and Chronic Changes and Predictive Value of Tpeak-Tend lor Ventricular Arrhythmia Risk in Cardiac Resynchronization Therapy Patients显示文摘 | Cong Xue Wei Hua Chi Cai Li-Gang Ding Zhi-Min Liu Xiao-Han Fan Yun-Zi Zhao Shu Zhang | 2016 | Chinese Medical Journal2016，，18： | 6 |
| 4 | Surveillance on the Immune Effectiveness of Quadrivalent and Trivalent Split Influenza Vaccines—Shenzhen City and Changzhou City,China,2018–2019显示文摘Summary What is already known about this topic?Vaccinations are the most effective way to prevent influenza virus infections and severe outcomes.Influenza vaccine effectiveness can vary by seasons.What is added by this report?This report monitors the antibody level among the population over time after administration of the quadrivalent or trivalent split influenza vaccine.What are the implications for public health practice?Real-time monitoring of serum antibody changes after vaccination provides important data for the development of reasonable and effective strategies for influenza prevention and control. | Liumei Shu Jie Zhang Xiang Huo Cong Chen Shisong Fang Kexin Zong Yuanyuan Guo Yingze Zhao Jun Zhang Changjun Bao Junjie Xia Fengcai Zhu Dayan Wang Guizhen Wu George F.Gao William J.Liu | 2020 | China CDC weekly2020，2，21： | 5 |
| 5 | Predictors of super-response to cardiac resynchronization therapy: the significance of heart failure medication, pre-implant left ventricular geometry and high percentage of biventricular pacing显示文摘BackgroundSuper 应答者(SR ) 被定义为在心脏的再同步治疗(CRT ) 以后显示出关键心脏的功能改进的病人。这研究的目的是在 CRT.MethodsThis 学习注册了从 2010 ~ 2014 在时期期间经历了 CRT 的 201 个病人以后,识别并且验证 SR 的预言者。临床并且 echocardiographic 评估在 CRT 和 6 个月前被进行在以后。有在纽约心协会(NYHA ) 的减少的病人功能的班 1,在左室的结束收缩的体积(LVESV ) 的减少 15% ,并且最后的左室的喷射部分(LVEF ) 当经历了 CRT 的 201 个病人的 SRs.Results29% 作为 SR 被识别, 45% 被分类。在基线, SR 有显著地更小的左 atrial 直径(男孩) , LVESV,左室的结束心脏舒张的体积(LVEDV ) 和比 non-super-responders (non-SRs ) 的更高的 LVEF。用变换血管收缩素的酶禁止者或血管收缩素受体 blockers (ACEI/ARB ) 的病人的百分比比 non-SRs 在 SR 是更高的。大多数 SR 有 Biventricular (BiV ) 在 CRT 以后在比 98% 六个月大的百分比上踱步。在 multivariate 逻辑回归分析, SR 的独立预言者是更低的 LVEDV [奇怪的比率(或) :0.93;信心间隔(CI ) :0.90-0.97 ] , ACEI/ARB 的使用(或:0.33;CI:0.13-0.82 ) 并且比 98% 大的 BiV 踱步百分比(或:0.29;CI:0.16-0.87 ) 有 ACEI/ARB 和更少 ectatic 的更好的依从的 .ConclusionPatients 在 CRT 前的室的几何学趋于有成为 SR 的更大的可能性。BiV 踱步的更高的百分比为成为 SR 是必要的。 | Han JIN Min GU Wei HUA Xiao-Han FAN Hong-Xia NIU Li-Gang DING Jing WANG Cong XUE Shu ZHANG | 2017 | Journal of Geriatric Cardiology2017，14，12： | 4 |
| 6 | Characteristics of mucosa-associated gut microbiota during treatment in Crohn's disease显示文摘BACKGROUND The dysbiosis of the gut microbiome is evident in Crohn's disease(CD) compared with healthy controls(HC), although the alterations from active CD to remission after treatment are unclear.AIM To characterize the mucosa-associated gut microbiota in patients with CD before and after the induction therapy.METHODS The basic information was collected from the subjects and the CD activity index(CDAI) was calculated in patients. A 16S rRNA sequencing approach was applied to determine the structures of microbial communities in mucosal samples including the terminal ileal, ascending colon, descending colon and rectum. The composition and function of mucosa-associated gut microbiota were compared between samples from the same cohort of patients before and after treatment.Differential taxa were identified to calculate the microbial dysbiosis index(MDI)and the correlation between MDI and CDAI was analyzed using Pearson correlation test. Predictive functional profiling of microbial communities was obtained with PICRUSt.RESULTS There were no significant differences in microbial richness among the four anatomical sites in individuals. Compared to active disease, the alpha diversity of CD in remission was increased towards the level of HC compared to the active stage. The principal coordinate analysis revealed that samples of active CD were clearly separated from those in remission, which clustered close to HC. Sixty-five genera were identified as differentially abundant between active and quiescent CD, with a loss of Fusobacterium and a gain of potential beneficial bacteria including Lactobacillus, Akkermansia, Roseburia, Ruminococcus and Lachnospira after the induction of remission. The combination of these taxa into a MDI showed a positive correlation with clinical disease severity and a negative correlation with species richness. The increased capacity for the inferred pathways including Lipopolysaccharide biosynthesis and Lipopolysaccharide biosynthesis proteins in patients before treatment negatively correlated with the abundance of Roseburia,Ruminococcus and Lachnospira.CONCLUSION The dysbiosis of mucosa-associated microbiota was associated with the disease phenotype and may become a potential diagnostic tool for the recurrence of disease. | Cong He Huan Wang Wang-Di Liao Chao Peng Xu Shu Xuan Zhu Zhen-Hua Zhu | 2019 | World Journal of Gastroenterology2019，25，18： | 3 |
| 7 | Emerging of two-dimensional materials in novel memristor显示文摘The rapid development of big-data analytics(BDA),internet of things(IoT)and artificial intelligent Technology(AI)demand outstanding electronic devices and systems with faster processing speed,lower power consumption,and smarter computer architecture.Memristor,as a promising Non-Volatile Memory(NVM)device,can effectively mimic biological synapse,and has been widely studied in recent years.The appearance and development of two-dimensional materials(2D material)accelerate and boost the progress of memristor systems owing to a bunch of the particularity of 2D material compared to conventional transition metal oxides(TMOs),therefore,2D material-based memristors are called as new-generation intelligent memristors.In this review,the memristive(resistive switching)phenomena and the development of new-generation memristors are demonstrated involving graphene(GR),transition-metal dichalcogenides(TMDs)and hexagonal boron nitride(h-BN)based memristors.Moreover,the related progress of memristive mechanisms is remarked. | Zhican Zhou Fengyou Yang Shu Wang Lei Wang Xiaofeng Wang Cong Wang Yong Xie Qian Liu | 2022 | Frontiers of physics2022，17，2： | 3 |
| 8 | A novel dimeric procyanidin glucoside from Polygonum aviculare显示文摘新奇二聚的 procyanidin 配糖物, catechin 3-O-acetate-(48 )-catechin 3-O-acetate-3-O--d-glucopyranoside (1 ) ,与五一起, flavonoids 和一 lignan 从蓼 aviculare 的天线部分被孤立。他们的结构被分光镜的分析包括 1D, 2D NMR, MS 和 CD 方法阐明。 | Hai Jian Cong Shu Wei Zhang Chong Zhang Yu Jie Huang Li Jiang Xuan | 2012 | Chinese Chemical Letters2012，23，7： | 2 |
| 9 | Study on the bioactivity changes of hydroxylated sulfonylureas derivatives: A possible metabolism显示文摘Some new sulfonylureas and their hydroxylation products had been synthesized from 2-amino-4-methylpyrimidine. Their bioactivities against E. coli AHAS II in vitro were tested and the results indicated that the hydroxylation decreased the inhibition activities of sulfonylureas significantly. Subsequently herbicidal tests against stem-growth of barnyard grass and root-growth of rape confirmed the above conclusion. The preliminary molecular docking studies were also carried out to investigate the binding modes of non-hydroxylated and hydroxylated sulfonylureas with AHAS. | Shu Rong Ban Cong Wei Niu Wen Bin Chen Zhi Hong Yu Si Wu Chen Wang Zhen Xi | 2007 | Chinese Chemical Letters2007，18，2： | 2 |
| 10 | Synthesis of monodispersed Fe3O4@C core/shell nanoparticles显示文摘We report a facile method to synthesize dispersed Fe_3O_4@C nanoparticles(NPs). Fe_3O_4 NPs were firstly prepared via the high temperature diol thermal decomposition method. Fe_3O_4@C NPs were fabricated using glucose as a carbon source by hydrothermal process. The obtained products were characterized by X-ray diffraction(XRD), transmission electron microscopy(TEM), vibrating sample magnetometer(VSM) and Raman spectra. The results indicate that the original shapes and magnetic property of Fe_3O_4 NPs can be well preserved. The magnetic particles are well dispersed in the carbon matrix. This strategy would provide an efficient approach for existing applications in Li-ion batteries and drug delivery. Meanwhile, it offers the raw materials to assemble future functional nanometer and micrometer superstructures. | Rui Liu Shu Mi Yuanyuan Li Cong Chen Yong Xie Qiang Chen Ziyu Chen | 2016 | Science China Chemistry2016，59，4： | 2 |
| 11 | Reciprocal repression between TUSC 7 and mi R ‐23b in gastric cancer显示文摘 | Peng Qi Mi‐die Xu Xiao‐Han Shen Shu‐Juan Ni Dan Huang Cong Tan Wei‐Wei Weng Wei‐Qi Sheng Xiao‐Yan Zhou Xiang Du | 2015 | Cancer2015，，6： | 1 |
| 12 | ROS is essential for initiation of energy deprivation-induced autophagy显示文摘Autophagy is a highly conserved degradation process in which intracellular components are engulfed into a double membrane structure,known as the autophagosomes,and then delivered to lysosomes/vacuoles for degradation(Ohsumi,2014).Recently,autophagy has been found to be responsible for maintaining glucose homeostasis in adult animals(Karsli-Uzunbas et al.,2014).However,the molecular mechanism underlying energy deprivation-induced autophagy remains relatively unclear. | Choufei Wu Yixing Li Shu Zhong Yingcong Chen Yu Xie Yuyao Feng Weijing Yao Suping Fu Yanlan Zhu Liefeng Wang Yuting Chen Liqin Zhang Jingjing Tong Cong Yi | 2021 | Journal of Genetics and Genomics2021，48，6： | 1 |
| 13 | Characterization and Genome Sequencing of a Novel Coliphage Isolated from Engineered Escherichia coli显示文摘 | Li Shu Liu Lina Zhu Junmin Zou Lingyun Li Ming Cong Yanguang Rao Xiancai Hu Xiaomei Zhou Yingbing Chen Zhijin Hu Fuquan | 2010 | Intervirology2010，，4： | 1 |
| 14 | Anterior chamber angle imaging with swept-source optical coherence tomography: an investigation on variability of angle measurement显示文摘 | LIU Shu YU M YE Cong | 2011 | Invest Ophthalmol Vis Sci2011，52，12： | 1 |
| 15 | Prevalence of High-Risk Human Papillomavirus in Cervical Intraepithelial Neoplasia in the Pre-Vaccine Era—China,2017–2018显示文摘What is already known about this topic?Human papillomavirus(HPV)type-specific strategies play a key role in the prevention of cervical intraepithelial neoplasia(CIN),but evidence on the HPV type-specific prevalence in CIN is limited.What is added by this report?This multicentric study estimates the prevalence of high-risk HPV types in CIN cases and the associations of HPV infection patterns with high-grade CIN in China in 2017–2018.What are the implications for public health practice?Population-based prevention strategies should give preference to HPV-16 and 18,and strategies for non-16/18 high-risk HPV are essential for the elimination of cervical cancer. | Heling Bao Weihong Chen Xiaosong Zhang Hui Bi Yun Zhao Liwen Fang Shu Cong Feng Tan Linhong Wang | 2022 | China CDC weekly2022，4，48： | 1 |
| 16 | Peripheral nerve fibroblasts secrete neurotrophic factors to promote axon growth of motoneurons显示文摘Peripheral nerve fibroblasts play a critical role in nerve development and regeneration.Our previous study found that peripheral nerve fibroblasts have different sensory and motor phenotypes.Fibroblasts of different phenotypes can guide the migration of Schwann cells to the same sensory or motor phenotype.In this study,we analyzed the different effects of peripheral nerve-derived fibroblasts and cardiac fibroblasts on motoneurons.Compared with cardiac fibroblasts,peripheral nerve fibroblasts greatly promoted motoneuron neurite outgrowth.Transcriptome analysis results identified 491 genes that were differentially expressed in peripheral nerve fibroblasts and cardiac fibroblasts.Among these,130 were significantly upregulated in peripheral nerve fibroblasts compared with cardiac fibroblasts.These genes may be involved in axon guidance and neuron projection.Three days after sciatic nerve transection in rats,peripheral nerve fibroblasts accumulated in the proximal and distal nerve stumps,and most expressed brain-derived neurotrophic factor.In vitro,brain-derived neurotrophic factor secreted from peripheral nerve fibroblasts increased the expression ofβ-actin and F-actin through the extracellular regulated protein kinase and serine/threonine kinase pathways,and enhanced motoneuron neurite outgrowth.These findings suggest that peripheral nerve fibroblasts and cardiac fibroblasts exhibit different patterns of gene expression.Peripheral nerve fibroblasts can promote motoneuron neurite outgrowth. | Qian-Ru He Meng Cong Fan-Hui Yu Yu-Hua Ji Shu Yu Hai-Yan Shi Fei Ding | 2022 | Neural Regeneration Research2022，17，8： | 1 |
| 17 | Cardiac resynchronization therapy improved the clinical outcomes in pacemaker patients upgraded to biventricular device显示文摘 | Han JIN Wei HUA Li-Gang DING Jing WANG Hong-Xia NIU Min GU Cong XUE Shu ZHANG | 2017 | Journal of Geriatric Cardiology2017，14，10： | 1 |
| 18 | Intra-host Ebola viral adaption during human infection显示文摘The onsite next generation sequencing(NGS)of Ebola virus(EBOV)genomes during the 2013–2016 Ebola epidemic in Western Africa provides an opportunity to trace the origin,transmission,and evolution of this virus.Herein,we have diagnosed a cohort of EBOV patients in Sierra Leone in 2015,during the late phase of the outbreak.The surviving EBOV patients had a recovery process characterized by decreasing viremia,fever,and biochemical parameters.EBOV genomes sequenced through the longitudinal blood samples of these patients showed dynamic intra-host substitutions of the virus during acute infection,including the previously described short stretches of 13 serial TNC mutations.Remarkably,within individual patients,samples collected during the early phase of infection possessed Ts at these nucleotide sites,whereas they were replaced by Cs in samples collected in the later phase,suggesting that these short stretches of TNC mutations could emerge independently.In addition,up to a total of 35 nucleotide sites spanning the EBOV genome were mutated coincidently.Our study showed the dynamic intra-host adaptation of EBOV during patient recovery and gave more insight into the complex EBOV-host interactions. | William JLiu Weifeng Shi Wuyang Zhu Cong Jin Shumei Zou Ji Wang Yuehua Ke Xiaofeng Li Mi Liu Tao Hu Hang Fan Yigang Tong Xiang Zhao Wenbin Chen Yuhui Zhao Di Liu Gary Wong Chengchao Chen Chunyu Geng Weiwei Xie Hui Jiang Idrissa Laybor Kamara Abdul Kamara Matt Lebby Brima Kargbo Xiangguo Qiu Yu Wang Xiaofeng Liang Mifang Liang Xiaoping Dong Guizhen Wu George F.Gao Yuelong Shu | 2019 | Biosafety and Health2019，1，1： | 1 |
| 19 | Identification of in-vivo induced genes of Streptococcus suis serotype 2 specially expressed in infected human显示文摘 | Shu Li Jie Song Hui Huang Wei Chen Ming Li Yan Zhao Yanguang Cong Junmin Zhu Xiancai Rao Xiaomei Hu Fuquan Hu | 2013 | Microbial Pathogenesis2013，，： | 1 |
| 20 | Vehicle classification in pan-tilt-zoom videos via sparse learning显示文摘 | Cong Wu Bo Li Shu Shen | 2013 | Journal of Electronic Imaging2013，22，4： | 1 |
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