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| 1 | The Tea Tree Genome Provides Insights into Tea Flavor and Independent Evolution of Caffeine Biosynthesis显示文摘茶是世界有巨大的经济、药用、文化的重要性的最旧、很流行的包含咖啡因的饮料。这里,我们在场第一个高质量的核苷酸序列充满重复(80.9%) ,栽培的茶树山茶 sinensis 的 3.02-Gb 染色体。我们证明茶树的一种非常地大的染色体尺寸从一些 LTR retrotransposon 家庭的慢、稳定、长期的扩大被结果。除了一个最近的整个染色体的复制事件,基因的系特定的扩大把新陈代谢的生合成与 flavonoid 联系了被发现,它提高 catechin 生产,萜烯酶激活,和应力公差,为茶风味和改编的重要特征。我们相对可可子和咖啡表明一个独立人士和茶咖啡因合成小径的快速的进化。在 25 山茶种类之中的比较研究表明那更高的表情大多数 flavonoid- 和咖啡因铺平 -- 然而并非 theanine 相关的基因贡献 catechins 和咖啡因的增加的生产并且因此提高处理茶的适用性和茶质量。这些新奇调查结果为进一步的 metabolomic 和典型生合成小径的功能的 genomic 精炼铺平道路并且将帮助开发将最后满足并且吸引世界范围的更多的茶喝酒者的茶风味的一个更多样化的集合。 | En-Hua Xia Hai-Bin Zhang Jun Sheng Kui Li Qun-Jie Zhang Changhoon Kim Yun Zhang Yuan Liu Ting Zhu Wei Li Hui Huang Yan Tong Hong Nan Cong Shi Chao Shi Jian-Jun Jiang Shu-Yan Mao Jun-Ying Jiao Dan Zhang Yuan Zhao You-Jie Zhao Li-Ping Zhang Yun-Long Liu Ben-Ying Liu Yue Yu Sheng-Fu Shao De-Jiang Ni Evan E. Eichler Li-Zhi Gao | 2017 | Molecular Plant2017，10，6： | 113 |
| 2 | Raman Spectroscopy and Imaging of Graphene显示文摘Graphene has many unique properties that make it an ideal material for fundamental studies as well as for potential applications.Here we review recent results on the Raman spectroscopy and imaging of graphene.We show that Raman spectroscopy and imaging can be used as a quick and unambiguous method to determine the number of graphene layers.The strong Raman signal of single layer graphene compared to graphite is explained by an interference enhancement model.We have also studied the effect of substrates,the top layer deposition,the annealing process,as well as folding(stacking order)on the physical and electronic properties of graphene.Finally,Raman spectroscopy of epitaxial graphene grown on a SiC substrate is presented and strong compressive strain on epitaxial graphene is observed.The results presented here are highly relevant to the application of graphene in nano-electronic devices and help in developing a better understanding of the physical and electronic properties of graphene. | Zhenhua Ni Yingying Wang Ting Yu Zexiang Shen | 2008 | Nano Research2008，1，4： | 51 |
| 3 | An intriguing RNA species--perspectives of circularized RNA显示文摘圆形的 RNA (circRNAs ) ,一种 covalently 关上的 RNA 分子,被认为拼接 mis 事件的副产品的一种类型并且在早年里由于工艺的限制偶发地被发现。与象高产量的下一代的定序那样的大工艺的进步,众多的 circRNAs 最近在许多种类被检测了。CircRNAs 以一种时间空间地特定的方式被表示,建议他们的规章的功能的潜力以前被俯看。有趣地,某 circRNAs 确实在某些情形与批评生理的功能被发现。CircRNAs 有能抵抗到比作那些线性的 exoribonuclease 的更稳定的分子的结构,并且他们的分子的函数包括 microRNA 海绵,在抄写的规章的角色,与线性拼接竞争的 mRNA 陷井,为翻译并且可能的模板另外的一会儿未知的角色。这里,我们考察 circRNAs,起源和形成机制,生理的功能和分子的角色的发现和描述,与为 circRNAs 的察觉的方法一起。我们进一步调查未来并且建议关键问题为这些魔术的 RNA 分子被回答。 | Ting Shen Miao Han Gang Wei Ting Ni | 2015 | Protein & Cell2015，6，12： | 29 |
| 4 | Prevalence of Thyroid Nodules and Its Relationship with Iodine Status in Shanghai:a Population-based Study显示文摘Objective This study was designed to evaluate the prevalence of thyroid nodules(TNs) and its relationship with urine iodine concentrations(UICs) after the regional rapid economic growth and lifestyle changes. Methods A cross-sectional survey was conducted in the general population aged 15-69 years. A questionnaire regarding general and personal characteristics and relevant information was administered. Ultrasonography of the thyroid was performed, and serum triiodothyronine(T3), tetraiodothyronine(T4), serum thyroid stimulating hormone(TSH), free triiodothyronine(FT3), free tetraiodothyronine(FT4), thyroglobulin antibody(Tg Ab), thyroid peroxidase antibody(TPOAb), and TSH receptor antibody(TRAb) levels were measured for each individual subject. Results The prevalence rates of TNs in the whole population, females and males were 27.76%, 34.04%, and 21.60%, respectively. The prevalence of multiple nodules increased with age, whereas the prevalence peaks differed between males and females. The median UICs in the whole population and females with non-TNs were higher than those of subjects with TNs(P=0.0035, P=0.0068). The median UICs in subjects with a single TN were higher than those in subjects with multiple TNs(P=0.0164, P=0.0127). The result showed a U-shaped curve relationship between UIC and prevalence of TNs. The prevalence of TNs was the lowest when the UIC was 140-400 μg/L. Conclusion The prevalence of TNs was nearly 30% and increased with age. The relationship between UIC and prevalence of TNs is U-shaped, with an increase in risk when the UIC was <140 μg/L and >400 μg/L. Very low or high UIC levels need attention and correction. | SONG Jun ZOU Shu Rong GUO Chang Yi ZANG Jia Jie ZHU Zhen Ni MI Ming HUANG Cui Hua YU Hui Ting LU Xi RUAN Ye WU Fan | 2016 | Biomedical and Environmental Sciences2016，29，6： | 25 |
| 5 | Comparative analysis of the complete chloroplast genome among Prunus mume,P.armeniaca,and P.salicina显示文摘Prunus mume Sieb.et Zucc.,P.armeniaca L.,and P.salicina L.are economically important fruit trees in temperate regions.These species are taxonomically perplexing because of shared interspecific morphological traits and variation,which are mainly attributed to hybridization.The chloroplast is cytoplasmically inherited and often used for evolutionary studies.We sequenced the complete chloroplast genomes of P.mume,P.armeniaca,and P.salicina using Illumina sequencing followed by de novo assembly.The three chloroplast genomes exhibit a typical quadripartite structure with conserved genome arrangement,structure,and moderate divergence.The lengths of the genomes are 157,815,157,797,and 157,916 bp,respectively.The length of the large single-copy region(LSC)region is 86,113,86,283,and 86,122 bp,and the length of the SSC region is 18,916,18,734,and 19,028 bp;the IR region is 26,393,26,390,and 26,383 bp,respectively.Each of the three chloroplast genomes encodes 133 genes,including 94 protein-coding,31 tRNA,and eight rRNA genes.Differential gene analysis for the three species revealed that trnY-ATA is a unique gene in P.armeniaca;in contrast,the gene trnI-TAT is only present in P.mume and P.salicina,though the position of the gene in these chloroplast genomes differs.Further comparative analysis of the complete chloroplast genome sequences revealed that the ORF genes and the sequences of linked regions rps16 and atpA,atpH and atpI,trnc-GCA and psbD,ycf3 and atpB,and rpL32 and ndhD are significantly different and may be used as molecular markers in taxonomic studies.Phylogenetic evolution analysis of the three species suggests that P.mume has a closer genetic relationship to P.armeniaca than to P.salicina. | Song Xue Ting Shi Wenjie Luo Xiaopeng Ni Shahid Iqbal Zhaojun Ni Xiao Huang Dan Yao Zhijun Shen Zhihong Gao | 2019 | Horticulture Research2019，6，1： | 10 |
| 6 | Rat model of cholelithiasis with human gallstones implanted in cholestasis-induced virtual gallbladder显示文摘AIM:To facilitate translational research on cholelithiasis,we have developed a rat model of human gallstones by exploiting the unique biliopancreatic features of this species.METHODS:Under anesthesia,16 adult rats of equal genders underwent two times of abdominal surgery.First,their common bile duct(CBD)was ligated to cause cholestasis by total biliary obstruction(TBO).On day 0,1,3,7,14,21 and 28 after TBO,magnetic resonance imaging(MRI)was conducted to monitor the dilatation of the CBD,and blood was sampled to analyze total serum bilirubin(TSB).Secondly,on day 30,the abdomen was re-opened and gallstone(s)collected from human patients were implanted in the dilated CBD asa virtual gallbladder(VGB),which was closed by suture ligation.This rat cholelithiasis model was examined by MRI,clinical observation,microcholangiography and histology.RESULTS:All rats survived two laparotomies.After ligation,the CBD was dilated to a stable size of 4 to30 mm in diameter on day 21-28,which became a VGB.The rats initially showed signs of jaundice that diminished over time,which paralleled with the evolving TSB levels from 0.6±0.3 mg/d L before ligation,through a peak of 10.9±1.9 mg/dL on day 14,until a nearly normalized value after day 28.The dilated CBD with thickened wall allowed an incision for implantation of human gallstones of 1-10 mm in diameter.The rat cholelithiasis was proven by in vivo MRI and postmortem microcholangiography and histomorphology.CONCLUSION:A rat model cholelithiasis with human gallstones has been established,which proves feasible,safe,reliable,nontoxic and cost-effective.Given the gallstones of human origin,applications of this model may be of help in translational research such as optical detection and lysis of gallstones by systemic drug administration. | Marlein Miranda Cona Yewei Liu Ting Yin Yuanbo Feng Feng Chen Stefaan Mulier Yue Li Jian Zhang Raymond Oyen Yicheng Ni | 2016 | World Journal of Methodology2016，6，2： | 7 |
| 7 | A small-dose naloxone infusion alleviates nausea and sedation without impacting analgesia via intravenous tramadol显示文摘背景早研究证明那 naloxone 注入减少从静脉内的控制病人的痛觉缺失的吗啡相关的副作用的发生。当最小化静脉内的 tramadol 管理引起的副作用时,这研究试图决定 naloxone 是否保存了痛觉缺失。为颈的 vertebrae 外科经历一般麻醉的八十个病人随机被划分成四个组的方法。所有病人收到了 30 在外科的结束前纪录的 1 mg/kg tramadol,与 0.3 mgkg-1 由连续注入列在后面吗?? | JIA Dong-lin NI Cheng XU Ting ZHANG Li-ping GUO Xiang-yang | 2010 | Chinese Medical Journal2010，，13： | 7 |
| 8 | Superior in vitro anticancer effect of biomimetic paclitaxel and triptolide co-delivery system in gastric cancer显示文摘As a well-known anticancer drug,paclitaxel(PTX),a first-line chemotherapeutic agent,remains unsatisfactory for gastric cancer therapy.It is reported that triptolide(TPL)could enhance the anti-gastric cancer effect of PTX.Considering the poor solubility of both drugs,we developed a red blood cell membrane-biomimetic nanosystem,an emerging tool in drug delivery,to co-load paclitaxel and triptolide(red blood cell membrane coated PTX and TPL co-loaded poly(lactic-co-glycolic acid)[PLGA]nanoparticles,RP(P/T)).The successful preparation was confirmed in terms of particle size,morphology,and surface markers assays.This biomimetic system could prolong circulation and escape immune surveillance.And these properties were verified by stability,in vitro drug release,and cellular uptake assays.Moreover,the MTT and colony formation assays demonstrated the superior anti-proliferation effect of the RP(P/T)to free drugs.The enhanced antitumor effects of RP(P/T)on migration and invasion were also evaluated by wound-healing and transwell assays.Overall,the bionic co-delivery nanoplatform with improved efficacy in vitro is a promising therapy for gastric cancer. | Siwan Wang Hui Jiang Jia Wang Haisi Wu Ting Wu Mengnan Ni Qianqian Zhao You Ji Ziting Zhang Chunming Tang Huae Xu | 2021 | The Journal of Biomedical Research2021，35，4： | 5 |
| 9 | Serine metabolism orchestrates macrophage polarization by regulating the IGF1–p38 axis显示文摘Serine metabolism is reportedly involved in immune cell functions, but whether and how serine metabolism regulates macrophage polarization remain largely unknown. Here, we show that suppressing serine metabolism, either by inhibiting the activity of the key enzyme phosphoglycerate dehydrogenase in the serine biosynthesis pathway or by exogenous serine and glycine restriction, robustly enhances the polarization of interferon-γ-activated macrophages (M(IFN-γ)) but suppresses that of interleukin-4-activated macrophages (M(IL-4)) both in vitro and in vivo. Mechanistically, serine metabolism deficiency increases the expression of IGF1 by reducing the promoter abundance of S-adenosyl methionine-dependent histone H3 lysine 27 trimethylation. IGF1 then activates the p38-dependent JAK–STAT1 axis to promote M(IFN-γ) polarization and suppress STAT6-mediated M(IL-4) activation. This study reveals a new mechanism by which serine metabolism orchestrates macrophage polarization and suggests the manipulation of serine metabolism as a therapeutic strategy for macrophage-mediated immune diseases. | Xiao Shan Penghui Hu Lina Ni Long Shen Yanan Zhang Zemin Ji Yan Cui Meihua Guo Haoan Wang Liyuan Ran Kun Yang Ting Wang Lei Wang Bin Chen Zhi Yao Vingjie Wu Qiujing Yu | 2022 | Cellular & Molecular Immunology2022，19，11： | 5 |
| 10 | Microscopic view of phytoplankton along the Yangtze River显示文摘Phytoplankton play an important role in nutrient cycling and energy conversion. However, phytoplankton community is poorly understood in large river ecosystems. Based on the large-scale samplings conducted in spring and autumn of 2017, a systematic study has been made on seasonal and spatial distributions of phytoplankton community along the mainstream of the Yangtze River. As results, 6 phyla were identified and 59 phytoplankton species were detected using microscopy, including Bacillariophyta(29 species), Chlorophyta(13 species) and Cyanophyta(8 species) as the dominant group. Moreover, the density of phytoplankton was ranged 0.05×10^6–1.90×10^6 cells/L and the biomass was 0.01–1.01 mg/L, both reaching their maximum in the middle reach from Yichang to Wuhan. Significant seasonal difference of phytoplankton community was observed and spatial dissimilarity in spring was revealed. The cell abundance and biomass of phytoplankton could be fairly estimated by chlorophylla, and redundancy analysis indicated that water temperature, dissolved oxygen, biochemical oxygen demand, nitrite, and ammonia nitrogen were the most important explanatory environmental factors for phytoplankton compositions in the Yangtze River. | LIU YuXin XU XuMing WANG Ting NI JinRen | 2019 | Science China(Technological Sciences)2019，62，11： | 3 |
| 11 | Three Gorges Dam: friend or foe of riverine greenhouse gases?显示文摘Dams are often regarded as greenhouse gas(GHG) emitters. However, our study indicated that the world’s largest dam, the Three Gorges Dam(TGD), has caused significant drops in annual average emissions of CO, CHand NO over 4300 km along the Yangtze River, accompanied by remarkable reductions in the annual export of CO(79%), CH(50%) and NO(9%) to the sea. Since the commencement of its operation in 2003, the TGD has altered the carbonate equilibrium in the reservoir area, enhanced methanogenesis in the upstream, and restrained methanogenesis and denitrification via modifying anoxic habitats through long-distance scouring in the downstream. These findings suggest that ‘large-dam effects’ are far beyond our previous understanding spatiotemporally, which highlights the fundamental importance of whole-system budgeting of GHGs under the profound impacts of huge dams. | Jinren Ni Haizhen Wang Tao Ma Rong Huang Philippe Ciais Zhe Li Yao Yue Jinfeng Chen Bin Li Yuchun Wang Maosheng Zheng Ting Wang Alistair G.L.Borthwick | 2022 | National Science Review2022，9，6： | 3 |
| 12 | Quality control of cell-based high-throughput drug screening显示文摘The pharmaceutical industry is presently suffering difficult times due to low productivity of new molecular entities.As a major source of drug leads,high-throughput screening(HTS)has been often criticized for its‘dead end’lead compounds.However,the fruitful achievements resulting from HTS technology indicate that it remains a feasible way for drug innovation.Because of increasing considerations of earlier stage ADMET(absorption,distribution,metabolism,excretion and toxicity)in drug development,cell-based HTS is highly recommended in modern drug discovery for its ability to detect more biologically relevant characteristics of compounds in living systems.This review provides a systematic and practical description of vital points for conducting high quality cell-based HTS,from assay development to optimization,compound management,data analyses,hit validation as well as lead identification.Potential problems and solutions are also covered. | Zhiyun Zhang Ni Guan Ting Li Dale E.Mais Mingwei Wang | 2012 | Acta Pharmaceutica Sinica B2012，2，5： | 3 |
| 13 | Effect of GAPT Extract on Expression of Tau Protein and Its Phosphorylation Related Enzymes in Hippocampal Neurons of APPV717I Transgenic Mice显示文摘Objective:To investigate the effect of GAPT,an extract mixture from Radix Ginseng,Rhizoma Acor tatarinowii,Radix Polygalae and Radix Curcuma(containing ingredient of turmeric),etc.on expression of tau protein and its phosphorylation related enzyme in hippocampal neurons of APPV717I transgenic mice.Methods:Sixty three-month-old APPV717I transgenic mice were randomly divided into model group,donepezil group[0.92 mg/(kg·d)],the low,medium and high dosage of GAPT groups[0.075,0.15,0.30 g/(kg·d),12 in each group],and 12 three-month-old C57BL/6J mice were set as a normal control group,treatments were administered orally once a day respectively,and both the normal group and model group were given 0.5%sodium carboxymethyl cellulose solution.Immunohistochemistry(IHC)and Western blot analysis were used to detect the expression of total tau protein(Tau-5),cyclin-dependent kinase 5(CDK5)and protein phosphatase 2A(PP2A)in hippocampal neurons of experimental mice after 8-month drug administration(11 months old).Results:In the model group,the expression of Tau-5 and CDK5 were increased,whereas the expression of PP2A was decreased in hippocampal neurons,which were significantly different compared with that in the normal group(all P<0.01).IHC test indicated the number and area of either Tau-5 or CDK5 positive cells were decreased with a dose-depended way in GAPT groups,and an increase of PP2A.Compared with the model group,the changes were significant in GAPT groups(P<0.05 or P<0.01).Similar results were shown by Western blot.Conclusion:GAPT could attenuate abnormal hyperphosphorylation of tau protein in hippocampal neurons of APPV717I transgenic mice via inhibiting the expression of CDK5 and activating the expression of PP2A. | NI Jing-nian SHI Jing ZHANG Xue-kai YANG Yi-chang LIU Xiao-meng WEI Ming-qing LI Ting WANG Peng-wen TIAN Jin-zhou WANG Yong-yan | 2017 | Chinese Journal of Integrative Medicine2017，23，8： | 3 |
| 14 | Therapeutic strategies for chronic wound infection显示文摘Chronic wounds have always been a tough fight in clinical practice,which can not only make patients suffer from pain physically and mentally but also impose a heavy burden on the society.More than one factor is relevant to each step of the development of chronic wounds.Along with the in-depth research,we have realized that figuring out the pathophysiological mechanism of chronic wounds is the foundation of treatment,while wound infection is the key point concerned.The cause of infection should be identified and prevented promptly once diagnosed.This paper mainly describes the mechanism,diagnosis and therapeutic strategies of chronic wound infection,and will put an emphasis on the principle of debridement. | Yi-Fan Liu Peng-Wen Ni Yao Huang Ting Xie | 2022 | Chinese Journal of Traumatology2022，25，1： | 3 |
| 15 | Isolation of a novel characterized Issatchenkia terricola from red raspberry fruits on the degradation of citric acid and enrichment of flavonoid and volatile profiles in fermented red raspberry juice显示文摘High content of citric acid in red raspberry juice leads to poor sensory experience. This study developed a feasible method to degrade citric acid in red raspberry juice using a novel characterized Issatchenkia terricola WJL-G4 isolated from red raspberry fruits. I. terricola WJL-G4 exhibited a potent capability of reducing the citric acid contents from(22.8 ± 0.08) g/L to(6.2 ± 0.02) g/L within 36 h fermentation, then completely depleted after 48 h. Furthermore, the contents of phenolic compound, including neochlorogenic acid, p-coumaric acid, raspberry ketone, and rutin significantly increased after 36 h fermentation. Fermentation increased total flavonoid contents in red raspberry juice, compared to that in control group. Volatile profiles exhibited to be enriched after fermentation, which contributed to the improvement of the juice taste. Our findings showed that I. terricola WJL-G4 can be applied in deacidification, enrichment of flavonoid compounds and volatile profiles in fermented red raspberry juice.. | Ying Jiang Ting Luo Ying Tang Sirui Chen Hui Ni Qihe Chen Xingshun Song Yihong Bao Zeyuan Deng Jinling Wang | 2022 | Food Science and Human Wellness2022，11，4： | 3 |
| 16 | A single-center retrospective long-term analysis of 80 cases of ovarian Sertoli-Leydig cell tumors显示文摘To the Editor:Sertoli-Leydig cell tumors(SLCTs)are a rare type of sex cord-stromal tumor of the ovary,accounting for<0.5%of all ovarian tumors.[1]An accurate diagnosis and the knowledge of prognosis based on clinical and pathological features have important therapeutic implications;however,the rarity of SLCT renders collecting reliable data difficult.We aimed to analyze the features of SLCTs to clarify their relationship with the behavior of the tumor and explore reasonable modes of therapy. | Ni Yang Ting Gui Dong-Yan Cao Keng Shen Yan You | 2021 | Chinese Medical Journal2021，，19： | 3 |
| 17 | Recent strategies on targeted delivery of thrombolytics显示文摘Thrombus formed in blood vessel is a progressive process, which would lead to lifethreatening thrombotic diseases such as ischemic stroke. Unlike other diseases, the recognition of thrombus is usually in the late stage where blood vessels are largely blocked. So acute thrombotic diseases have a narrow therapeutic window, and remain leading causes of morbidity and mortality, whereas current thrombolysis therapy has limited therapeutic effects and bleeding complications. Thrombolytic agents in unwanted sites would cause hemorrhage due to the activation of plasminogen. Moreover, untargeted thrombolysis therapy require large amounts of thrombolytic agents, which in return would enhance hemorrhage risk. To improve the efficiency while minimizing the adverse effects of traditional thrombolysis therapy, novel drug delivery systems have been investigated. Various targeting strategies including ultrasound and magnetic field directed targeting, and specific binding, have been designed to deliver thrombolytic drugs to the thrombotic sites. These strategies demonstrate promising results in reducing bleeding risk as well as allowing less dosage of thrombolytic drugs with lowered clot lysis time. In this review, we discuss recent progress on targeted delivery of thrombolytics, and summarize treatment advantages and shortcomings, potentially helping to further promote the development of targeted thrombolysis. | Ting Huang Ni Li Jianqing Gao | 2019 | Asian Journal of Pharmaceutical Sciences2019，14，3： | 3 |
| 18 | Pancreatic imaging:Current status of clinical practices and small animal studies显示文摘Different causative factors acting on the pancreas can result in diseases such as pancreatitis, diabetes and pancreatic tumors. The high incidence and mortality of pancreatic diseases have placed diagnostic imaging in a crucial position in daily clinical practice. In this minireview article different pancreatic imaging techniques are discussed, from the standard clinical imaging modalities and state of the art clinical magnetic resonance imaging techniques to current situations in pre-clinical pancreatic imaging studies. In particular, the challenges of pre-clinical rodent pancreatic imaging are addressed, with both the image acquisition techniques and the post-processing methods for rodent pancreatic imaging elaborated. | Ting Yin Yewei Liu Ronald Peeters Yuanbo Feng Yicheng Ni | 2017 | World Journal of Methodology2017，7，3： | 2 |
| 19 | Large primary hepatocellular carcinoma: Transarterial chemoembolization monotherapy versus combined transarterial chemoembolization‐percutaneous microwave coagulation therapy显示文摘 | Lin‐Feng Xu Hong‐Liang Sun Yao‐Ting Chen Jia‐Yan Ni Dong Chen Jiang‐Hong Luo Jing‐Xing Zhou Ren‐Mei Hu Qi‐Yun Tan | 2013 | J Gastroenterol Hepatol2013，，： | 2 |
| 20 | Effects of substituting chemical fertilizers with manure on rice yield and soil labile nitrogen in paddy fields of China: A meta-analysis显示文摘Substituting chemical fertilizers with manure is an important method for efficient nutrient management in rice cropping systems of China.Labile nitrogen(N)is the most active component of the soil N pool and plays an essential role in soil fertility.However,the effects of manure substitution on soil labile N in rice cropping systems and their relationships with soil properties,fertilization practices,and climatic conditions remain unclear and should be systematically quantified.Here,we investigated rice grain yield and four types of soil labile N that have been widely reported,including available nitrogen(AN),ammonium nitrogen(NH_(4)^(+)-N),nitrate nitrogen(NO_(3)^(−)-N),and microbial biomass nitrogen(MBN).We reviewed 187 published articles and performed a meta-analysis to quantify the effects of manure substitution on yield and soil labile N.The results showed that manure substitution increased AN,MBN,NH+4-N,and NO−3-N by 11.3%,38.5%,5.9%,and 8.1%,respectively.Partial substitution significantly increased the yield by 1.4%–5.9%,but full substitution significantly decreased the yield by 2.9%.The positive effects of manure substitution on yield and AN were stronger with long-term fertilization.The differences in responses varied across specific manure types,N application rates,soil properties,and climatic factors.In conclusion,manure substitution can increase soil labile N and is regarded as an efficient strategy for improving soil N fertility and a recommended measure for applying both chemical and organic fertilizers in rice systems.This study provides evidence of the effects of manure substitution on yield enhancement by increasing soil labile N. | Qiong HOU Yuemin NI Shan HUANG Ting ZUO Jian WANG Wuzhong NI | 2023 | Pedosphere2023，33，1： | 2 |
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