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| 1 | AXL is a candidate receptor for SARS-CoV-2 that promotes infection of pulmonary and bronchial epithelial cells显示文摘The current coronavirus disease 2019(COVID-19)pandemic presents a global public health challenge.The viral pathogen responsible,severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),binds to the host receptor ACE2 through its spike(S)glycoprotein,which mediates membrane fusion and viral entry.Although the role of ACE2 as a receptor for SARS-CoV-2 is clear,studies have shown that ACE2 expression is extremely low in various human tissues,especially in the respiratory tract.Thus,other host receptors and/or co-receptors that promote the entry of SARS-CoV-2 into cells of the respiratory system may exist.In this study,we found that the tyrosine-protein kinase receptor UFO(AXL)specifically interacts with the N-terminal domain of SARS-CoV-2 S.Using both a SARS-CoV-2 virus pseudotype and authentic SARS-CoV-2,we found that overexpression of AXL in HEK293T cells promotes SARS-CoV-2 entry as efficiently as overexpression of ACE2,while knocking out AXL significantly reduces SARS-CoV-2 infection in HI 299 pulmonary cells and in human primary lung epithelial cells.Soluble human recombinant AXL blocks SARS-CoV-2 infection in cells expressing high levels of AXL.The AXL expression level is well correlated with SARS-CoV-2 S level in bronchoalveolar lavage fluid cells from COVID-19 patients.Taken together,our findings suggest that AXL is a novel candidate receptor for SARS-CoV-2 which may play an important role in promoting viral infection of the human respiratory system and indicate that it is a potential target for future clinical intervention strategies. | Shuai Wang Zongyang Qiu Yingnan Hou Xiya Deng Wei Xu Tingting Zheng Peihan Wu Shaofang Xie Weixiang Bian Chong Zhang Zewei Sun Kunpeng Liu Chao Shan Aifu Lin Shibo Jiang Youhua Xie Qiang Zhou Lu Lu Jing Huang Xu Li | 2021 | Cell Research2021，31，2： | 39 |
| 2 | RAB31 marks and controls an ESCRT-independent exosome pathway显示文摘Exosomes are generated within the multivesicular endosomes(MVEs)as intraluminal vesicles(ILVs)and secreted during the fusion of MVEs with the cell membrane.The mechanisms of exosome biogenesis remain poorly explored.Here we identify that RAB31 marks and controls an ESCRT-independent exosome pathway.Active RAB31,phosphorylated by epidermal growth factor receptor(EGFR),engages flotillin proteins in lipid raft microdomains to drive EGFR entry into MVEs to form ILVs,which is independent of the ESCRT(endosomal sorting complex required for transport)machinery.Active RAB31 interacts with the SPFH domain and drives ILV formation via the Flotillin domain of flotillin proteins.Meanwhile,RAB31 recruits GTPase-activating protein TBC1D2B to inactivate RAB7,thereby preventing the fusion of MVEs with lysosomes and enabling the secretion of ILVs as exosomes.These findings establish that RAB31 has dual functions in the biogenesis of exosomes:driving ILVs formation and suppressing MVEs degradation,providing an exquisite framework to better understand exosome biogenesis. | Denghui Wei Weixiang Zhan Ying Gao Liyan Huang Run Gong Wen Wang Ruhua Zhang Yuanzhong Wu Song Gao Tiebang Kang | 2021 | Cell Research2021，31，2： | 12 |
| 3 | Characteristics of dissolved organic matter in lakes with different eutrophic levels in southeastern Hubei Province,China显示文摘Dissolved organic matter(DOM)plays a crucial role in both the carbon cycle and geochemical cycles of other nutrient elements,which is of importance to the management and protection of the aquatic environments.To achieve a more comprehensive understanding the characteristics of DOM in the Changjiang(Yangtze)River basin,water samples from four natural lakes(Xiandao,Baoan,Daye,and Qingshan)in southeastern Hubei Province in China with different eutrophication levels were collected and analyzed.The optical characteristics were analyzed using ultraviolet-visible spectrophotometry and excitation-emission matrix spectroscopy coupled with parallel factor analysis.The results show that:(1)two humic-like components(C1 and C2)and two protein-like substances(C3 and C4)of DOM were identified in all waterbodies;(2)C3 originated primarily from the degradation of microalgae and contributed substantially to humic-like components during transformation.C4 was widely present in the Changjiang River basin and its formation was related to microbial activity,rather than algal blooms or seasons.Influenced by the water mixing,the protein-like components were more likely to be transformed by microorganism,whereas humic-like components were more easily to be photobleached;(3)the concentration of DOM and the fluorescence intensity of humic-like components gradually increased with rising lake eutrophication levels.With respect to protein-like components,only C3 showed changes along the eutrophication gradients;(4)DOM showed a high affinity with permanganate index(COD Mn)and chlorophyll a(chl a)while the relationship was variable with phosphorus.This study helps us systematically understand the DOM characteristics,microbial activities,and pollutant transformation in the Changjiang River basin and provides reference to the ecological restoration of aquatic environments. | Weixiang REN Xiaodong WU Xuguang GE Guiying LIN Mengdie ZHOU Zijie LONG Xinhui YU Wei TIAN | 2021 | Journal of Oceanology and Limnology2021，39，4： | 8 |
| 4 | Removal of nitrogen from wastewater with perennial ryegrass/artificial aquatic mats biofilm combined system显示文摘To develop a cost-effective combined phytoremediation and biological process,a combined perennial ryegrass/artificial aquatic mat biofilm reactor was used to treat synthetic wastewater.Influent ammonium loading,reflux ratio,hydraulic retention time(HRT) and temperature all had significant effects on the treatment efficiency.The results indicated that the effluent concentration of ammonium increased with increasing influent ammonium loading.The reactor temperature played an important role in the nitrification process.The ammonium removal efficiency significantly decreased from 80% to 30%-50% when the reactor temperature dropped to below 10°C.In addition,the optimal nitrogen removal condition was a reflux ratio of 2.The nitrate and ammonium concentration of the effluent were consistent with the HRT of the combined system.The chemical oxygen demand(COD) removal efficiency was at a high level during the whole experiment,being almost 80% after the start-up,and then mostly above 90%.The direct uptake of N by the perennial ryegrass accounted for 18.17% of the total N removal by the whole system.The perennial ryegrass absorption was a significant contributor to nitrogen removal in the combined system.The result illustrated that the combined perennial ryegrass/artificial aquatic mat biofilm reactor demonstrated good performance in ammonium,total N and COD removal. | Chongjun Chen Rui Zhang Liang Wang Weixiang Wu Yingxu Chen | 2013 | Journal of Environmental Sciences2013，25，4： | 5 |
| 5 | Economical evaluation of large-scale photovoltaic systems using Universal Generating Function techniques显示文摘Solar energy plays an important role in the global energy framework for future.Comparing with conventional generation systems using fossil fuels,the cost structure of photovoltaic(PV)systems is different:the capital cost is higher while the operation cost is negligible.Reliabilities of the PV system can also influence the cost for producing electricity.Investors,planners and regulators require deep insight into the return and cost of a PV project.A reliability based economical assessment of largescale PV systems has been conducted utilizing Universal Generating Function(UGF)techniques.The reliability models of solar panel arrays,PV inverters and energy production units(EPUs)are represented as the corresponding UGFs.The expected energy production models for different PV system configurations have also been developed.The expected unit cost of electricity has been calculated to provide informative metrics for making optimal decisions.The proposed method has been applied to determine the PV system configuration which provides electricity for a water purification process. | Yi DING Weixiang SHEN Gregory LEVITIN Peng WANG Lalit GOEL Qiuwei WU | 2013 | Journal of Modern Power Systems and Clean Energy2013，1，2： | 3 |
| 6 | Exploring long-term effects of biochar on mitigating methane emissions from paddy soil:a review显示文摘Biochar has been reported to mitigate short-term methane(CH4)emissions from paddy soil.Currently,CH4 mitigation by biochar has primarily focused on the abundance and variations of methanogens and methanotrophs,and changes in their activities during methane production and consumption.However,long-term effects of biochar on methane mitigation from paddy soil remain controversial.This review overviewed the existing mechanisms for CH4 mitigation as a result of biochar application.In addition,the two existing opinions on the long-term CH4 mitigation effect upon biochar application were highlighted.Combining the already explored mechanisms of fresh biochar on CH4 mitigation from paddy soil and a novel discovery,the potential mechanisms of biochar on long-term methane emission response were proposed.This review also revealed the uncertain responses of biochar on long-term CH4 mitigation.Therefore,to achieve carbon neutral goal,it is important to further explore the mechanisms of long-term CH4 mitigation under biochar application. | Qiong Nan Liqing Xin Yong Qin Muhammad Waqas Weixiang Wu | 2021 | Biochar2021，3，2： | 3 |
| 7 | Transgenic Bt rice does not affect enzyme activities and microbial composition in the rhizosphere during crop development显示文摘 | Wei Liu Hao Hao Lu Weixiang Wu Qi Kun Wei Ying Xu Chen Janice E. Thies | 2007 | Soil Biology and Biochemistry2007，，2： | 2 |
| 8 | Modeling a web-based remote monitoring and fault diagnosis system with UML and component technology显示文摘 | Xing Wu Jin Chen Ruqiang Li Weixiang Sun Guicai Zhang Fucai Li | 2006 | Journal of Intelligent Information Systems2006，，1： | 1 |
| 9 | Reduction of nitrogen loss and Cu and Zn mobility during sludge composting with bamboo charcoal amendment显示文摘 | Li Hua Weixiang Wu Yuxue Liu Murray B. McBride Yingxu Chen | 2009 | Environmental Science and Pollution Research2009，，1： | 1 |
| 10 | Identification of immune cell infiltration landscape for predicting prognosis of colorectal cancer显示文摘Background:The tumor microenvironment plays an essential role in the therapeutic response to immunotherapy.It is necessary to identify immune cell infiltration(ICI)subtypes for evaluating prognosis and therapeutic benefits.This study aimed to evaluate the ICI score as an effective prognostic biomarker for immune response.Methods:The cell-type identification by estimating relative subsets of RNA transcripts and the estimation of stromal and immune cells in malignant tumors using expression methods were used to analyse ICI landscapes in 161 colorectal cancer(CRC)samples with patients’clinical and prognostic data,RNA sequencing data,and whole-exome sequencing data from the Sixth Affiliated Hospital,Sun Yat-sen University(Guangzhou,China).Statistical analysis and data processing were conducted to calculate ICI scores,and to analyse the prognosis of CRC patients with different ICI scores and other features.A similar analysis with RNA sequencing and clinical data of colon adenocarcinoma(COAD)samples from The Cancer Genome Atlas(TCGA)database was conducted to confirm the correctness of the findings.Results:The high-ICI score group with a better prognosis(hazard ratio[HR],2.19;95%confidence interval[CI],1.03–4.64;logrank test,P=0.036)was characterized by the increased tumor mutational burden and interleukin-17(IL-17)signaling pathway.Significant differences in the prognosis and the expression levels of immune checkpoints and chemokine marker genes were found between the two ICI score groups.For COAD samples from TCGA,the results also showed a significant difference in patients’prognosis between the two ICI score groups(HR,1.72;95%CI,1.00–2.96;log-rank test,P=0.047).Conclusions:Tumor heterogeneity induced differences in identifying ICI subtypes of CRC patients.The ICI score may serve as an effective biomarker for predicting prognosis,help identify new therapeutic markers for CRC,and develop novel effective immune checkpoint blockade therapies. | Xueqian Wu Jianxia Li Yuanzhe Zhang Yi Cheng Zehua Wu Weixiang Zhan Yanhong Deng | 2023 | Gastroenterology Report2023，11，1： | 1 |
| 11 | Correlation of reactor performance and bacterial community composition during the removal of trimethylamine in three-stage biofilters显示文摘 | Ying Ding Weixiang Wu Zhiying Han Yingxu Chen | 2007 | Biochemical Engineering Journal2007，，2： | 1 |
| 12 | Modeling a web-based remote monitoring and fault diagnosis system with UML and component technology显示文摘 | Xing Wu Jin Chen Ruqiang Li Weixiang Sun Guicai Zhang Fucai Li | 2006 | Journal of Intelligent Information Systems2006，，1： | 1 |
| 13 | Correlation of reactor performance and bacterial community composition during the removal of trimethylamine in three-stage biofilters显示文摘 | Ying Ding Weixiang Wu Zhiying Han Yingxu Chen | 2007 | Biochemical Engineering Journal2007，，2： | 1 |
| 14 | Pharmacological inhibition of BAP1 recruits HERC2 to competitively dissociate BRCA1-BARD1, suppresses DNA repair and sensitizes CRC to radiotherapy显示文摘Radiotherapy is widely used in the management of advanced colorectal cancer(CRC).However,the clinical efficacy is limited by the safe irradiated dose.Sensitizing tumor cells to radiotherapy via interrupting DNA repair is a promising approach to conquering the limitation.The BRCA1-BARD1 complex has been demonstrated to play a critical role in homologous recombination(HR)DSB repair,and its functions may be affected by HERC2 or BAP1.Accumulated evidence illustrates that the ubiquitination-deubiquitination balance is involved in these processes;however,the precise mechanism for the cross-talk among these proteins in HR repair following radiation hasn’t been defined.Through activity-based profiling,we identified PT33 as an active entity for HR repair suppression.Subsequently,we revealed that BAP1 serves as a novel molecular target of PT33 via a CRISPR-based deubiquitinase screen.Mechanistically,pharmacological covalent inhibition of BAP1 with PT33 recruits HERC2 to compete with BARD1 for BRCA1 interaction,interrupting HR repair.Consequently,PT33 treatment can substantially enhance the sensitivity of CRC cells to radiotherapy in vitro and in vivo.Overall,these findings provide a mechanistic basis for PT33-induced HR suppression and may guide an effective strategy to improve therapeutic gain. | Xin Yue Tingyu Liu Xuecen Wang Weijian Wu Gesi Wen Yang Yi Jiaxin Wu Ziyang Wang Weixiang Zhan Ruirui Wu Yuan Meng Zhirui Cao Liyuan Le Wenyan Qiu Xiaoyue Zhang Zhenyu Li Yong Chen Guohui Wan Xianzhang Bu Zhenwei Peng Ran-yi Liu | 2023 | Acta Pharmaceutica Sinica B2023，13，8： | 1 |
| 15 | Reduction of nitrogen loss and Cu and Zn mobility during sludge composting with bamboo charcoal amendment显示文摘 | Li Hua Weixiang Wu Yuxue Liu Murray B. McBride Yingxu Chen | 2009 | Environmental Science and Pollution Research2009，，1： | 1 |
| 16 | Evaluation of biochar effects on nitrogen retention and leaching in Multi-layered soil columns显示文摘 | Ding Y Liu Yuxue Wu Weixiang | 2010 | Water Air Soil Pollt2010，213，1234： | 1 |
| 17 | Impacts upon soil quality and plant growth of bamboo charcoal addition to composted sludge显示文摘 | Hua Li Chen Yingxu Wu Weixiang | 2012 | Environmental Technology2012，33，1： | 1 |
| 18 | Evaluation of biochar effects on nitrogen retention and leaching in multi-layered soil columns显示文摘 | Ding Ying Liu Yuxue Wu Weixiang | 2010 | Water Air and Soil Pollution2010，213，14： | 1 |
| 19 | Chemical characterization of rice straw-derived biochar for soil amendment显示文摘 | Weixiang Wu Min Yang Qibo Feng Kim McGrouther Hailong Wang Haohao Lu Yingxu Chen | 2012 | Biomass and Bioenergy2012，，： | 1 |
| 20 | Pilot-scale nitrogen removal from leachate by ex situ nitrification and in situ denitrification in a landfill bioreactor显示文摘 | Faqian Sun Bin Sun Qian Li Xiaoya Deng Jian Hu Weixiang Wu | 2013 | Chemosphere2013，，： | 1 |
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