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| 1 | Transgenic rhesus monkeys carrying the human MCPH1 gene copies show human-like neoteny of brain development显示文摘Brain size and cognitive skills are the most dramatically changed traits in humans during evolution and yet the genetic mechanisms underlying these human-specific changes remain elusive.Here,we successfully generated 11 transgenic rhesus monkeys(8 first-generation and 3 second-generation)carrying human copies of MCPH1,an important gene for brain development and brain evolution.Brain-image and tissue-section analyses indicated an altered pattern of neural-cell differentiation,resulting in a delayed neuronal maturation and neural-fiber myelination of the transgenic monkeys,similar to the known evolutionary change of developmental delay(neoteny)in humans.Further brain-transcriptome and tissue-section analyses of major developmental stages showed a marked human-like expression delay of neuron differentiation and synaptic-signaling genes,providing a molecular explanation for the observed brain-developmental delay of the transgenic monkeys.More importantly,the transgenic monkeys exhibited better short-term memory and shorter reaction time compared with the wild-type controls in the delayed-matching-to-sample task.The presented data represent the first attempt to experimentally interrogate the genetic basis of human brain origin using a transgenic monkey model and it values the use of non-human primates in understanding unique human traits. | Lei Shi Xin Luo Jin Jiang Yongchang chen Cirong Liu Ting Hu Min Li Qiang Lin Yanjiao Li Jun Huang Hong Wang Yuyu Niu Yundi Shi Martin Styner Jianhong Wang Yi Lu Xuejin Sun Hualin Yu Weizhi Ji Bing Su | 2019 | National Science Review2019，6，3： | 12 |
| 2 | Influences of working pressure on properties for TiO_2 films deposited by DC pulse magnetron sputtering显示文摘TiO2 films were deposited at room temperature by DC pulse magnetron sputtering system.The crystalline structures,morphological features and photocatalytic activity of TiO2 films were systematically investigated by X-ray diffraction(XRD),atomic force microscopy(AFM) and ultraviolet spectrophotometer,respectively.The results indicated that working pressure was the key deposition parameter in?uencing the TiO2 film phase composition at room temperature,which directly affected its photocatalytic activity.With increasing working pressure,the target self-bias decreases monotonously.Therefore,low temperature TiO2 phase(anatase) could be deposited with high working pressure.The anatase TiO2 films deposited with 1.4 Pa working pressure displayed the highest photocatalytic activity by the decomposition of Methyl Orange solution,which the degradation rate reached the maximum(35%) after irradiation by ultraviolet light for 1 h. | ZHANG Can DING Wanyu WANG Hualin CHAI Weiping JU Dongying | 2009 | Journal of Environmental Sciences2009，21，6： | 11 |
| 3 | Techno-Economic Challenges of Fuel Cell Commercialization显示文摘随着资源稀缺、气候变化加剧以及污染程度上升等问题的凸显,经济增长需要依赖更加环保、高效的生产过程。燃料电池具有高效和环保的特点,是内燃机和锅炉的理想替代品,也是通向绿色工业的途径。然而,作为一种新的能源技术,燃料电池的市场渗透率仍然不高。本文通过研究生命周期和价值链活动,对燃料电池系统进行了技术经济和环境方面的分析。首先,我们研究了燃料电池的发展进程,根据其生命周期活动、价值链活动以及最终用户验收标准确定了下一阶段需要采取的行动。然后,我们统一了解了燃料电池商业化的制度障碍。最终用户的主要验收标准是功能、价格和可靠性,比起其他同类产品,如内燃机和电池,燃料电池需要在这些标准上有所提升,以凸显其竞争优势。燃料电池的维修和保养费用可能会使成本大幅增加并减小可用性(由于可靠性较低),而成本和可用性是最终用户能否接受的主要因素。因此,为了克服可靠性的障碍,燃料电池产业面临着巨大的挑战。本文深入洞察了过去几年燃料电池商业化面临的主要障碍,讨论了燃料电池在未来绿色低碳经济中的潜在关键作用。文中还提出了未来低碳经济的必要性,并指出了相关的研究方向。以燃料电池为配置的绿色能源可以真正作为未来的商业模式。通过采取绿色公众投资、实行政策举措,为经济增长提供一条更加持续的发展道路,鼓励更多的环保产业投资。 | Junyewang Hualin Wang Yi Fan | 2018 | Engineering2018，4，3： | 11 |
| 4 | Spatiotemporal differences and convergence of urban industrial land use efficiency for China's major economic zones显示文摘Based on an SBM model, this paper first analyzes the spatial differences of urban industrial land use efficiency(UILUE) in the six main economic zones of China. Then, we analyze the dynamic changes in the urban industrial land's total factor productivity(UILTFP) by using the Malmquist productivity index approach, and we examine the UILTFP's convergence. The results show that the Pearl River Delta and the Yangtze River Delta have a higher UILUEs but show downward trends in UILTFPs. The other four zones, Beijing-Tianjin-Hebei, Chengdu-Chongqing, Guanzhong-Tianshui and the Central Plains, show poorer UILUEs but have upward trends in UILTFPs. A significant excess supply of industrial land and labor exists in all six zones, and the zones in economically developed areas perform better in industrial output. The results of the convergence test do not support σ-convergence but support conditional β-convergence for all zones. These results indicate that the gaps in the UILTFP of industrial land in all six zones, in fact, do not narrow, and the UILTFPs of cities will converge to their own steady states. The regression results of the influencing factors show that the urbanization rate should be increased in the Pearl River Delta and reduced in Beijing-Tianjin-Hebei. The Yangtze River Delta and Chengdu-Chongqing should pay more attention to the problem of industrial labor surplus, and the Pearl River Delta should reduce the proportion of industrial output. All zones should focus on improving the quality of the industrial economy and the land use intensity. | XIE Hualin WANG Wei | 2015 | Journal of Geographical Sciences2015，25，10： | 8 |
| 5 | Virus like particle-based vaccines against emerging infectious disease viruses显示文摘Emerging infectious diseases are major threats to human health.Most severe viral disease outbreaks occur in developing regions where health conditions are poor.With increased international travel and business,the possibility of eventually transmitting infectious viruses between different countries is increasing.The most effective approach in preventing viral diseases is vaccination.However,vaccines are not currently available for numerous viral diseases.Viruslike particles(VLPs) are engineered vaccine candidates that have been studied for decades.VLPs are constructed by viral protein expression in various expression systems that promote the selfassembly of proteins into structures resembling virus particles.VLPs have antigenicity similar to that of the native virus,but are non-infectious as they lack key viral genetic material.VLP vaccines have attracted considerable research interest because they offer several advantages over traditional vaccines.Studies have shown that VLP vaccines can stimulate both humoral and cellular immune responses,which may offer effective antiviral protection.Here we review recent developments with VLP-based vaccines for several highly virulent emerging or re-emerging infectious diseases.The infectious agents discussed include RNA viruses from different virus families,such as the Arenaviridae,Bunyaviridae,Caliciviridae,Coronaviridae,Filoviridae,Flaviviridae,Orthomyxoviridae,Paramyxoviridae,and Togaviridae families. | Jinliang Liu Shiyu Dai Manli Wang Zhihong Hu Hualin Wang Fei Deng | 2016 | Virologica Sinica2016，31，4： | 7 |
| 6 | Stabilizing nickel sulfide nanoparticles with an ultrathin carbon layer for improved cycling performance in sodium ion batteries显示文摘Nanostructured 金属硫化物是为钠离子电池的潜在的电极材料;然而,他们典型地由于大体积变化和分泌物产品的溶解受不了很差的骑车的稳定性。此处,我们建议合理材料设计策略让基于硫化物的材料处理这些问题。拿镍硫化物(是的 NiS x ) 一个例子,我们证明它的电气化学的性能能被限制 NiS 在滤的 x nanoparticles 传导性的碳 nanotube 网络,并且与 ultrathin 碳涂层层稳定他们。碳层用作一个物理障碍减轻体积变化和活跃材料的溶解的效果。混合材料展出了一个大可逆特定的能力 > 超过 200 的 500 mAh/g 和优秀骑车稳定性骑车。作为电池阳极材料给 NiS x 的传统地有问题的性质,我们相信这里报导的观察高效反映我们的材料设计策略的有效性。 | Feipeng Zhao Qiufang Gong Brian Traynor Duo Zhang Jiaojiao Li Hualin Ye Fengjiao Chen Na Han Yeyun Wang Xuhui Sun Yanguang Li | 2016 | Nano Research2016，9，10： | 6 |
| 7 | A validated UPLC–MS/MS method for simultaneous determination of imatinib, dasatinib and nilotinib in human plasma显示文摘sensitive, rapid, simple and economical ultra-performance liquid chromatography–tandem mass spectrometric method(UPLC–MS/MS) was developed and validated for simultaneous determination of imatinib,dasatinib and nilotinib in human plasma using gliquidone as internal standard(IS). Liquid-liquid extraction method with ethyl acetate was used for sample pre-treatment. The separation was performed on an Xtimate Phenyl column using isocratic mobile phase consisting of A(aqueous phase: 0.15% formic acid and 0.05% ammonium acetate) and B(organic phase: acetonitrile)(A:B=40:60, v/v). The flow rate was 0.25 mL/min and the total run time was 6 min. The multiple reaction monitoring(MRM) transitions, m/z 494.5→394.5 for imatinib, 488.7→401.5 for dasatinib, 530.7→289.5 for nilotinib and 528.5→403.4 for IS, were chosen to achieve high selectivity in the simultaneous analyses. The method exhibited great improvement in sensitivity and good linearity over the concentration range of 2.6–5250.0 ng/mL for imatinib, 2.0–490.0 ng/mL for dasatinib, and 2.4–4700.0 ng/mL for nilotinib. The method showed acceptable results on sensitivity, specificity,recovery, precision, accuracy and stability tests. This UPLC–MS/MS assay was successfully used for human plasma samples analysis and no significant differences were found in imatinib steady-state trough concentrations among the SLC22A5-1889T > C or SLCO1B3 699G > A genotypes(P > 0.05). This validated method can provide support for clinical therapeutic drug monitoring and pharmacokinetic investigations of these three tyrosine kinase inhibitors(TKIs). | Jing Zeng Hualin Cai Zhiping Jiang Qing Wang Yan Zhu Ping Xu Xielan Zhao | 2017 | Journal of Pharmaceutical Analysis2017，7，6： | 6 |
| 8 | Exploration and practice of general practitioner responsibility system in an urban community of Shanghai显示文摘Objective:The Minhang district of Shanghai was selected as the subject and its public health management model was investigated.Methods:Minhang became a pilot location for the implementation of the general practitioner responsibility system(GPRS)in 2011.Since then,the management model of public health services in the district has gradually merged with the GPRS through a series of actions:the adjustment of organizations and the coordination mechanism in community public health services,the integration of basic public health services and clinic practice,the establishment of an informationized Internet service platform,comprehensive budgeting management of public health service programs,and performance assessment management of health workers.Results:By the end of 2014,the number of residents who had a contractual relationship with a general practitioner(GP)in Minhang had reached 1.4367 million and all of them had family health records.Compared with 2010,both the rate of maternal management and the rate of children management stayed at high level(≥96%),the rate of hypertension management increased(40.73%vs.28.70%),the rate of blood pressure control increased(82.13%vs.77.51%),the incidence of diabetes decreased(21.93%vs.37.85%),the rate of effective control of blood glucose for dia-betic patients increased(82.43%vs.62.77%),and the rate of child health management increased(99.79%vs.91.54%).Conclusion:The GPRS has helped to create a better community health environment.How-ever,the development of this system is limited by several factors,including the following:the shortage of GPs,most GPs do not have good public health service skills and a correct public health concept,unclear division of different services,and imperfect department coordination. | Yan Wu Yanping Zhao Xiaoxia Huang Junyan Wang Huilin Xu Hualin Su | 2015 | Family Medicine and Community Health2015，3，4： | 5 |
| 9 | Zika Virus Baculovirus-Expressed Virus-Like Particles Induce Neutralizing Antibodies in Mice显示文摘The newly emerged mosquito-borne Zika virus(ZIKV) strains pose a global challenge owing to its ability to cause microcephaly and neurological disorders. Several ZIKV vaccine candidates have been proposed, including inactivated and live attenuated virus vaccines, vector-based vaccines, DNA and RNA vaccines. These have been shown to be efficacious in preclinical studies in mice and nonhuman primates, but their use will potentially be a threat to immunocompromised individuals and pregnant women. Virus-like particles(VLPs) are empty particles composed merely of viral proteins, which can serve as a safe and valuable tool for clinical prevention and treatment strategies. In this study, we used a new strategy to produce ZIKV VLPs based on the baculovirus expression system and demonstrated the feasibility of their use as a vaccine candidate. The pre-membrane(prM) and envelope(E) proteins were co-expressed in insect cells and selfassembled into particles similar to ZIKV. We found that the ZIKV VLPs could be quickly and easily prepared in large quantities using this system. The VLPs were shown to have good immunogenicity in immunized mice, as they stimulated high levels of virus neutralizing antibody titers, ZIKV-specific IgG titers and potent memory T cell responses. Thus, the baculovirus-based ZIKV VLP vaccine is a safe, effective and economical vaccine candidate for use against ZIKV. | Shiyu Dai Tao Zhang Yanfang Zhang Hualin Wang Fei Deng | 2018 | Virologica Sinica2018，33，3： | 5 |
| 10 | Establishment of a Reverse Genetic System of Severe Fever with Thrombocytopenia Syndrome Virus Based on a C4 Strain显示文摘Severe fever with thrombocytopenia syndrome virus(SFTSV)is an emerging tick-borne bunyavirus that causes hemorrhagic fever-like disease(SFTS)in humans with a case fatality rate up to 30%.To date,the molecular biology involved in SFTSV infection remains obscure.There are seven major genotypes of SFTSV(C1-C4 and J1-J3)and previously a reverse genetic system was established on a C3 strain of SFTSV.Here,we reported successfully establishment of a reverse genetics system based on a SFTSV C4 strain.First,we obtained the 5’-and 3’-terminal untranslated region(UTR)sequences of the Large(L),Medium(M)and Small(S)segments of a laboratory-adapted SFTSV C4 strain through rapid amplification of cDNA ends analysis,and developed functional T7 polymerase-based L-,M-and S-segment minigenome assays.Then,fulllength cDNA clones were constructed and infectious SFTSV were recovered from co-transfected cells.Viral infectivity,growth kinetics,and viral protein expression profile of the rescued virus were compared with the laboratory-adapted virus.Focus formation assay showed that the size and morphology of the foci formed by the rescued SFTSV were indistinguishable with the laboratory-adapted virus.However,one-step growth curve and nucleoprotein expression analyses revealed the rescued virus replicated less efficiently than the laboratory-adapted virus.Sequence analysis indicated that the difference may be due to the mutations in the laboratory-adapted strain which are more prone to cell culture.The results help us to understand the molecular biology of SFTSV,and provide a useful tool for developing vaccines and antivirals against SFTS. | Mingyue Xu Bo Wang Fei Deng Hualin Wang Manli Wang Zhihong Hu Jia Liu | 2021 | Virologica Sinica2021，36，5： | 5 |
| 11 | The nucleoprotein of severe fever with thrombocytopenia syndrome virus processes a stable hexameric ring to facilitate RNA encapsidation显示文摘Severe fever with thrombocytopenia syndrome virus(SFTSV),a member of the Phlebovirus genus from the Bunyaviridae family endemic to China,is the causative agent of life-threatening severe fever with thrombocyto-penia syndrome(SFTS),which features high fever and hemorrhage.Similar to other negative-sense RNA viruses,SFTSV encodes a nucleocapsid protein(NP)that is essen-tial for viral replication.NP facilitates viral RNA encapsida-tion and is responsible for the formation of ribonucleopro-tein complex.However,recent studies have indicated that NP from Phlebovirus members behaves in inhomogene-ous oligomerization states.In the present study,we report the crystal structure of SFTSV NP at 2.8Åresolution and demonstrate the mechanism by which it processes a ring-shaped hexameric form to accomplish RNA encapsida-tion.Key residues essential for oligomerization are identi-fi ed through mutational analysis and identifi ed to have a signifi cant impact on RNA binding,which suggests that correct formation of highly ordered oligomers is a criti-cal step in RNA encapsidation.The fi ndings of this work provide new insights into the discovery of new antiviral reagents for Phlebovirus infection. | Honggang Zhou Yuna Sun Ying Wang Min Liu Chao Liu Wenming Wang Xiang Liu Le Li Fei Deng Hualin Wang Yu Guo Zhiyong Lou | 2013 | Protein & Cell2013，4，6： | 4 |
| 12 | Per os infectivity factors:a complicated and evolutionarily conserved entry machinery of baculovirus显示文摘Baculoviruses are a family of arthropod-specific large DNA viruses that infect insect species belonging to the orders Lepidoptera,Hymenoptera and Diptera.In nature,occlusion-derived viruses(ODVs) initiate baculovirus primary infection in the midgut epithelium of insect hosts,and this process is largely dependent on a number of ODV envelope proteins designated as per os infectivity factors(PIFs).Interestingly,PIF homologs are also present in other invertebrate large DNA viruses,which is indicative that per os infection is an ancient and phylogenetically conserved entry mechanism shared by these viruses.Here,we review the advances in the knowledge of the functions of individual PIFs and recent discoveries about the PIF complex,and discuss the evolutionary implications of PIF homologs in invertebrate DNA viruses.Furthermore,future research highlights on the per os infection mechanism are also prospected. | Xi Wang Xiaoping Liu George Alliwa Makalliwa Jiang Li Hualin Wang Zhihong Hu Manli Wang | 2017 | Science China(Life Sciences)2017，60，8： | 4 |
| 13 | Bubble size fractal dimension,gas holdup,and mass transfer in a bubble column with dual internals显示文摘As the scale of residual oil treatment increases and cleaner production improves in China,slurry bubble column reactors face many challenges and opportunities for residual oil hydrogenation technology.The internals development is critical to adapt the long-term stable operation.In this paper,the volumetric mass transfer coefficient,gas holdup and bubble size in a gas-liquid up-flow column are studied with two kinds of internals.The gas holdup and volumetric mass transfer coefficient increase by 120% and 42% when the fractal dimension of bubbles increases from 0.56 to 2.56,respectively.The enhanced mass transfer processing may improve the coke suppression ability in the slurry reactor for residual oil treatment.The results can be useful for the exploration of reacting conditions,scale-up strategies,and oil adaptability.This work is valuable for the design of reactor systems and technological processes. | Xiao Xu Junjie Wang Qiang Yang Lei Wang Hao Lu Honglai Liu Hualin Wang | 2020 | Chinese Journal of Chemical Engineering2020，28，12： | 4 |
| 14 | Phylogenetic analysis revealed the central roles of two African countries in the evolution and worldwide spread of Zika virus显示文摘Recent outbreaks of Zika virus(ZIKV) infections in Oceania's islands and the Americas were characterized by high numbers of cases and the spread of the virus to new areas. To better understand the origin of ZIKV, its epidemic history was reviewed. Although the available records and information are limited, two major genetic lineages of ZIKV were identified in previous studies.However, in this study, three lineages were identified based on a phylogenetic analysis of all virus sequences from Gen Bank, including those of the envelope protein(E) and non-structural protein 5(NS5) coding regions. The spatial and temporal distributions of the three identified ZIKV lineages and the recombination events and mechanisms underlying their divergence and evolution were further elaborated. The potential migration pathway of ZIKV was also characterized. Our findings revealed the central roles of two African countries, Senegal and Cote d'Ivoire, in ZIKV evolution and genotypic divergence. Furthermore, our results suggested that the outbreaks in Asia and the Pacific islands originated from Africa. The results provide insights into the geographic origins of ZIKV outbreaks and the spread of the virus, and also contribute to a better understanding of ZIKV evolution, which is important for the prevention and control of ZIKV infections. | Shu Shen Junming Shi Jun Wang Shuang Tang Hualin Wang Zhihong Hu Fei Deng | 2016 | Virologica Sinica2016，31，2： | 4 |
| 15 | Viral suppression of innate immunity via spatial isolation of TBK1/IKKε from mitochondrial antivirai platform显示文摘 | Yun-Jia Ning Manli Wang Maping Deng Shu Shen Wei Liu Wu-Chun Cao Fei Deng Yan-Yi Wang Zhihong Hu Hualin Wang | 2014 | Journal of Molecular Cell Biology2014，8，4： | 4 |
| 16 | Electroacupuncture promotes the proliferation of endogenous neural stem cells and oligodendrocytes in the injured spinal cord of adult rats显示文摘A contusive model of spinal cord injury at spinal segment T8-9 was established in rats. Huantiao (GB30) and Huatuojiaji (Ex-B05) were punctured with needles, and endogenous neural stem cells were labeled with 5-bromo-2'-deoxyuridine (BrdU) and NG2. Double immunofluorescence staining showed that electroacupuncture markedly increased the numbers of BrdU+/NG2+ cells at spinal cord tissue 15 mm away from the injury center in the rostral and caudal directions. The results suggest that electroacupuncture promotes the proliferation of endogenous neural stem cells and oligodendrocytes in rats with spinal cord injury. | Haiying Wu Min HU Dekai Yuan Yunhui Wang Jing Wang Tao Li Chuanyun Qian Hualin YU | 2012 | Neural Regeneration Research2012，7，15： | 4 |
| 17 | Alleviating mechanical degradation of hexacyanoferrate via strain locking during Na^(+) insertion/extraction for full sodium ion battery显示文摘Generation of large strains upon Na^(+) intercalation is one of the prime concerns of the mechanical degradation of Prussian blue(PB)and its analogs.Structural construction from the atomic level is imperative to maintain structural stability and ameliorate the long-term stability of PB.Herein,an inter nickel hexacyanoferrate(NNiFCN)is successfully introduced at the out layer of iron hexacyanoferrate(NFFCN)through ion exchange to improve structural stability through compressive stress locking by forming NNiFCN shell.Furthermore,the kinetics of sodium ion diffusion is enhanced through the built-in electric pathway.The electrochemical performance is therefore significantly improved with a remarkable long-term cycling stability over 3,000 cycles at 500 mA·g^(–1) in the full sodium-ion batteries(SIBs)with a maximum energy density of 91.94 Wh·g^(–1),indicating that the core-shell structured NNiFCN/NFFCN could be the low-cost and high-performance cathode for full SIBs in large-scale EES applications. | Jianguo Sun Hualin Ye Jin An Sam Oh Yao Sun Anna Plewa Yumei Wang Tian Wu Kaiyang Zeng Li Lu | 2022 | Nano Research2022，15，3： | 3 |
| 18 | A Note on 'Modules, Comodules, and Cotensor Products over Frobenius Algebras'显示文摘这是 Abram 的纸上的笔记“模块, Comodules,和 Cotensor Productsover Frobenius 代数学,代数学的杂志”(1999 ) 。用坐标最近由 Kadison 开发了的 Frobenius 的应用程序,一个人以 comultiplication 为 Frobeniusalgebras 有 Abram 的描述的一个直接证明(看见 L。Kadison (1999 )) 。为任何 Frobenius 代数学,由使用明确的 comultiplication,在模块的范畴和 comodules 的范畴之间的明确的通讯被获得。而且,与这,我们在对 cotensor 的角函子描述上给很简化的证明和 improveAbram 的结果函子。 | Xiaowu CHEN Hualin HUANG Yanhua WANG | 2006 | Chinese Annals of Mathematics,Series B2006，27，4： | 3 |
| 19 | Critical impeller speeds for a gas-inducing stirring tank loaded with solid particles显示文摘The influence of solid particles size,density and loading on the critical gas-inducing impeller speed was investigated in a gas–liquid–solid stirring tank equipped with a hollow Rushton impeller.Three types of solid particles,hollow glass beads with diameters of 300 μm,200 μm,100 μm,and 60 μm,silica gel and desalting resin,were used.It was found that the adding solid particles would change the critical impeller speed.For hollow glass beads and silica gel,whose relative densities were less than or equal to 1.5,the critical impeller speeds increased with the solid loading before reaching the maximum values,and then decreased to a value even lower than that without added solids.The size of the solids also had apparent influence on the critical impeller speed,and larger solid particles correspond to a smaller critical impeller speed.The experimental data also showed that the gasinducing was beneficial to the suspension of the solid particles. | Yanhong Zhang Zhengwei Zhang Chenwen Wei Hualin Wang | 2018 | Chinese Journal of Chemical Engineering2018，26，6： | 3 |
| 20 | Improving Baculovirus Transduction of Mammalian Cells by Incorporation of Thogotovirus Glycoproteins显示文摘Baculovirus can transduce a wide range of mammalian cells and is considered a promising gene therapy vector. However,the low transduction efficiency of baculovirus into many mammalian cells limits its practical application. Co-expressing heterologous viral glycoproteins(GPs), such as vesicular stomatitis virus G protein(VSV G), with baculovirus native envelope protein GP64 is one of the feasible strategies for improving virus transduction. Tick-borne thogotoviruses infect mammals and their GPs share sequence/structure homology and common evolutionary origins with baculovirus GP64.Herein, we tested whether thogotovirus GPs could facilitate the entry of the prototype baculovirus Autographa californica multiple multiple nucleopolyhedrovirus(AcMNPV) into mammalian cells. The gp genes of two thogotoviruses, Thogoto virus and Dhori virus, were inserted into the AcMNPV genome. Both GPs were properly expressed and incorporated into the envelope of the recombinant AcMNPVs. The transduction rates of recombinant AcMNPVs expressing the two thogotovirus GPs increased for approximately 4–12 fold compared to the wild type AcMNPV in six of the 12 tested mammalian cell lines. It seemed that thogotovirus GPs provide the recombinant AcMNPVs with different cell tropisms and showed better performance in several mammalian cells compared to VSV G incorporated AcMNPV. Further studies showed that the improved transduction was a result of augmented virus-endosome fusion and endosome escaping, rather than increased cell binding or internalization. We found the AcMNPV envelope protein GP64-mediated fusion was enhanced by the thogotovirus GPs at relatively higher p H conditions. Therefore, the thogotovirus GPs represent novel candidates to improve baculovirus-based gene delivery vectors. | Liangbo Hu Yimeng Li Fei Deng Zhihong Hu Hualin Wang Manli Wang | 2019 | Virologica Sinica2019，34，4： | 3 |
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