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| 1 | Development and molecular analysis of a novel acetohydroxyacid synthase rapeseed mutant with high resistance to sulfonylurea herbicides显示文摘With the increasing promotion of simplified rapeseed cultivation in recent years,the development of cultivars with high resistance to herbicides is urgently needed.We previously developed M342,which shows sulfonylurea herbicide resistance,by targeting acetohydroxyacid synthase(AHAS),a key enzyme in branched-chain amino acid synthesis.In the present study,we used a progeny line derived from M342 for an additional round of ethyl methane sulfonate mutagenesis,yielding the novel mutant DS3,which harbored two mutations in AHAS genes and showed high sulfonylurea resistance.One mutation was the substitution Trp574 Leu,as in M342,according to Arabidopsis protein sequencing.The other site was a newly recognized substitution,Pro197 Leu.A KASP marker targeting Pro197 Leu was developed and reliably predicted the response to sulfonylurea herbicides in the F2 population.The combination of Trp574 Leu and Pro197 Leu in DS3 produced a synergistic effect that greatly increased herbicide resistance.Analysis of the protein structures of AHAS1 and AHAS3 in wild-type and single-gene mutant plants revealed three-dimensional protein conformational changes that could account for differences in herbicide resistance characteristics including toxicity tolerance,AHAS enzyme activity,and AHAS gene expression. | Yue Guo Changle Liu Weihua Long Jianqin Gao Jiefu Zhang Song Chen Huiming Pu Maolong Hu | 2022 | The Crop Journal2022，10，1： | 3 |
| 2 | Derivation and characterization of human embryonic stem cell lines from poor quality embryos显示文摘Poor quality embryos discarded from in vitro fertilization (IVF) laboratories are good sources for deriving human embryonic stem cell (hESC) lines. In this study, 166 poor quality embryos donated from IVF centers on day 3 were cultured in a blastocyst medium for 2 days, and 32 early blastocysts were further cultured in a blastocyst optimum culture medium for additional 2 days so that the inner cell masses (ICMs) could be identified and isolated easily. The ICMs of 17 blastocysts were isolated by a mechanical method, while those of the other 15 blastocysts were isolated by immunosurgery. All isolated ICMs were inoculated onto a feeder layer for subcultivation. The rates of ICM at- tachment, primary ICM colony formation and the efficiency of hESC derivation were similar between the ICMs isolated by the two methods (P>0.05). As a result, four new hESC lines were established. Three cell lines had normal karyotypes and one had an unbalanced Robertsonian translocation. All cell lines showed normal hESC characteristics and had the differentiation ability. In conclusion, we established a stable and effective method for hESC isolation and culture, and it was confirmed that the mechanical isolation was an effective method to isolate ICMs from poor embryos. These results further indicate that hESC lines can be derived from poor quality embryos discarded by IVF laboratories. | Weiqiang Liu, Yifei Yin, Xiaolin Long, Yumei Luo, Yonghua Jiang, Wenhong Zhang, Hongzi Du, Shaoying Li, Yuhong Zheng, Qing Li, Xinjie Chen, Baoping Liao, Guohong Xiao, Weihua Wang, Xiaofang Sun Guangzhou Key Laboratory of Reproductive and Genetics Institute of Gynecology and Obstetrics, The Third Affiliated Hospital of Guangzhou Medical College, Guangzhou 510150, China | 2009 | Journal of Genetics and Genomics2009，36，4： | 3 |
| 3 | Detection of circulating tumor cells in peripheral blood from patients with gastric cancer using piRNAs as markers显示文摘 | Long Cui Yanru Lou Xinjun Zhang Hui Zhou Hongxia Deng Haojun Song Xiuchong Yu Bingxiu Xiao Weihua Wang Junming Guo | 2011 | Clinical Biochemistry2011，，13： | 2 |
| 4 | Fabrication of nanofibrous sensors by electrospinning显示文摘This article reviews the techniques and applications of electrospinning for the fabrication of nanofibrous sensors. Considering that nanosensors require a large specific surface area and a continuous structure for the conduction of current signals, electrospun nanofibers have the dominant advantage. The device preparation is mainly divided into surface treatment and high-temperature sintering, which are, respectively, used for preparing composite conductive fibers and inorganic semiconductor fibers. Typical applications include pressure sensing, gas sensing, photoelectric sensing, and temperature sensing. In addition, nano-selfpowered systems have been mentioned to emphasize the good performance of smart nanosystems that do not require external power. In addition, we have summarized some existing methods and suggestions for increasing the specific surface area and presented constructive ideas for the future development of these devices. | HAN WeiHua WANG YuZhi SU JianMin XIN Xin GUO YinDa LONG Yun-Ze RAMAKRISHNA Seeram | 2019 | Science China(Technological Sciences)2019，62，6： | 1 |
| 5 | Cloning, Expression and Characterization of the Human NOB1 gene显示文摘 | Yue Zhang Jun Ni Guangjin Zhou Jian Yuan Weihua Ren Yuxi Shan Wenwen Tang Long Yu Shouyuan Zhao | 2005 | Molecular Biology Reports2005，，3： | 1 |
| 6 | Tooth root regeneration using dental follicle cell sheets in combination with a dentin matrix - based scaffold显示文摘 | Bo Yang Gang Chen Jie Li Qing Zou Dan Xie Yali Chen Hang Wang Xiaohui Zheng Jie Long Wei Tang Weihua Guo Weidong Tian | 2011 | Biomaterials2011，，8： | 1 |
| 7 | Adsorption characteristics of water vapor on the hypercrosslinked polymeric adsorbent显示文摘 | Chao Long Ying Li Weihua Yu | 2012 | Chemical Engineering Journal2012，180，： | 1 |
| 8 | In situ resource utilization of lunar soil for highly efficient extraterrestrial fuel and oxygen supply显示文摘Building up a lunar settlement is the ultimate aim of lunar exploitation.Yet,limited fuel and oxygen supplies restrict human survival on the Moon.Herein,we demonstrate the in situ resource utilization of lunar soil for extraterrestrial fuel and oxygen production,which may power up our solely natural satellite and supply respiratory gas.Specifically,the lunar soil is loaded with Cu species and employed for electro catalytic CO_(2)conversion,demonstrating significant production of methane.In addition,the selected component in lunar soil(i.e.MgSiO_(3))loaded with Cu can reach a CH_(4)F aradaic efficiency of 72.05% with a CH_(4)production rate of 0.8 mL/min at 600 mA/cm^(2).Simultaneously,an O_(2)production rate of 2.3 mL/min can be achieved.Furthermore,we demonstrate that our developed process starting from catalyst preparation to electrocatalytic CO_(2)conversion is so accessible that it can be operated in an unmmaned manner via a robotic system.Such a highly efficient extraterrestrial fuel and oxygen production system is expected to push forward the development of mankind’s civilization toward an extraterrestrial settlement. | Yuan Zhong Jingxiang Low Qing Zhu Yawen Jiang Xiwen Yu Xinyu Wang Fei Zhang Weiwei Shang Ran Long Yingfang Yao Wei Yao Jun Jiang Yi Luo Weihua Wang Jinlong Yang Zhigang Zou Yujie Xiong | 2023 | National Science Review2023，10，2： | 1 |
| 9 | Cloning, Expression and Characterization of the Human NOB1 gene显示文摘 | Yue Zhang Jun Ni Guangjin Zhou Jian Yuan Weihua Ren Yuxi Shan Wenwen Tang Long Yu Shouyuan Zhao | 2005 | Molecular Biology Reports2005，，3： | 1 |
| 10 | Electrocatalytic oxidation treatment of phenol wastewater by Ti/α-PbO2/β-PbO2 electrode 显示文摘 | WANG Honghui YANG Weihua HUANG Long | 2008 | Journal of Huaqiao Uni- versity (Natural Science)2008，29，2： | 1 |
| 11 | Preparation, characterization and application of a copper (II)-bound polymeric ligand exchanger for selective removal of arsenate from water显示文摘 | Weihua Tao Aimin Li Chao Long Zhicheng Fan Wenwen Wang | 2011 | Journal of Hazardous Materials2011，，： | 1 |
| 12 | Unipolar resistive switching of Au+-implanted ZrO2 films显示文摘 | Liu Qi Long Shibing Guan Weihua Zhang Sen Liu Ming Chen Junning | 2009 | Journal of Semiconductors2009，，4： | 1 |
| 13 | Cloning, Expression and Characterization of the Human NOB1 gene显示文摘 | Yue Zhang Jun Ni Guangjin Zhou Jian Yuan Weihua Ren Yuxi Shan Wenwen Tang Long Yu Shouyuan Zhao | 2005 | Molecular Biology Reports2005，，3： | 1 |
| 14 | Tooth root regeneration using dental follicle cell sheets in combination with a dentin matrix - based scaffold显示文摘 | Bo Yang Gang Chen Jie Li Qing Zou Dan Xie Yali Chen Hang Wang Xiaohui Zheng Jie Long Wei Tang Weihua Guo Weidong Tian | 2011 | Biomaterials2011，，8： | 1 |
| 15 | Donor-acceptor 2D covalent organic frameworks for efficient heterogeneous photocatalyticα-oxyamination显示文摘Covalent organic frameworks(COFs)have received widespread interest due to their high porosity,excellent crystallinity,tailorable structures,and broad application prospects.It has been demonstrated that proper combination and arrangement of electron donor and acceptor units in 2D conjugated COF lattice could promote efficient charge separation and electron transfer,and thus is beneficial for photocatalysis.In this article,three donor-acceptor(D-A)2D COFs were prepared by Schiff base reaction of electron acceptor 4,4′,4′′,4′′′-(benzo[1,2-d:4,5-d′]bis(oxazole)-2,4,6,8-tetrayl)tetraaniline(BBO)with different electron donors:thieno[3,2-b]thiophene-2,5-dicarbaldehyde(TT),benzo[1,2-b:4,5-b’]dithiophene-2,6-dicarboxaldehyde(BDT)and terephthalaldehyde(Ph),respectively.These D-A 2D COFs exhibited prominent photocatalytic activity towardsα-oxyamination of 1,3-dicarbonyl with 2,2,6,6-tetramethyl-1-piperdinyloxy(TEMPO)upon visible light irradiation.Among these D-ABBO-COFs,DTT-ABBO-COF exhibited the highest photocatalytic rates,which can be ascribed to the more negative highest occupied molecular orbital(HOMO)and narrower bandgap.The excellent stability,high activity and superior recyclability render DTT-ABBO-COF as a potential and environmentally friendly heterogeneous catalyst forα-oxyamination. | Huan Liu Xiaoli Yan Weiben Chen Zhen Xie Shen Li Weihua Chen Ting Zhang Guolong Xing Long Chen | 2021 | Science China Chemistry2021，64，5： | 0 |
| 16 | Investigations of the mechanical response of dummy HTPB propellant grain under ultrahigh acceleration overload conditions using onboard flight-test measurements显示文摘In this paper,to study the mechanical responses of a solid propellant subjected to ultrahigh acceleration overload during the gun-launch process,specifically designed projectile flight tests with an onboard measurement system were performed.Two projectiles containing dummy HTPB propellant grains were successfully recovered after the flight tests with an ultrahigh acceleration overload value of 8100 g.The onboard-measured time-resolved axial displacement,contact stress and overload values were successfully obtained and analysed.Uniaxial compression tests of the dummy HTPB propellant used in the gunlaunched tests were carried out at low and intermediate strain rates to characterize the propellant's dynamic properties.A linear viscoelastic constitutive model was employed and applied in finite-element simulations of the projectile-launching process.During the launch process,the dummy propellant grain exhibited large deformation due to the high acceleration overload,possibly leading to friction between the motor case and propellant grain.The calculated contact stress showed good agreement with the experimental results,though discrepancies in the overall displacement of the dummy propellant grain were observed.The dynamic mechanical response process of the dummy propellant grain was analysed in detail.The results can be used to estimate the structural integrity of the analysed dummy propellant grain during the gun-launch process. | Yiming Zhang Ningfei Wang Weihua Ma Ran Wang Long Bai Yi Wu | 2024 | Defence Technology(防务技术)2024，32，2： | 0 |
| 17 | A Pyrazine-Based 2D Conductive Metal-Organic Framework for Efficient Lithium Storage显示文摘The merits of intrinsic electrical conductivity, high specific surface area, tunable chemical composition and tailor-made properties enable two-dimensional conductive metal-organic frameworks (2D c-MOFs) as promising next-generation electrode materials in the field of energy storage and conversion. Herein, we have designed and synthesized a novel pyrazine-based 2D c-MOF (TPQG-Cu-MOF) bearing extended π-conjugated structure and abundant redox active sites. Thanks to the excellent redox reversibility of pyrazine units and CuO2 units, as well as the insolubility of the rigid framework skeleton, TPQG-Cu-MOF as the cathode material of lithium-ion battery exhibits a reversible specific capacity (150.2 mAh·g–1 at 20 mAh·g–1), good cycling stability (capacity retention of 82.6% after 500 cycles at 1 A·g–1) and excellent rate performance. Comprehensive ex-situ spectroscopic studies revealed the reversible redox activity of pyrazine units and CuO2 units of TPQG-Cu-MOF during the Li+ insertion/extraction process. The deepening fundamental understanding of the structure-property relationship was proposed, which might pave the way for further development of efficient MOF-based energy storage devices. | Xiaoxiao Sun Xiaoli Yan Keming Song Ting Zhang Zongfan Yang Xi Su Weihua Chen Long Chen | 2023 | Chinese Journal of Chemistry2023，41，14： | 0 |
| 18 | Mean square stability of discrete-time linear systems with random impulsive disturbances显示文摘Dear editor,In the real world,many dynamic processes may experience abrupt state [1] or parameter changes. Mathematically,these abrupt changes of state can be modeled as ideal impulsive effects. And also,the moments at which impulses occur might be uncertain because they are triggered by unpredictable factors. | Jiamei LONG Yuqian GUO Weihua GUI | 2023 | Science China(Information Sciences)2023，66，6： | 0 |
| 19 | Effects of smart agricultural production investment announcements on shareholder value:Evidence from China显示文摘The advent of the era of the smart economy has made agricultural production more intelligent.An increasing number of companies have launched a series of investment activities aimed at smart agricultural production(SAP).However,whether smart agricultural production investment(SAPI)impacts the stock market has yet to be confirmed.Therefore,based on the sample data of 118 listed companies in China from 2010 to 2019,this study empirically examines the impact of SAPI announcements on shareholder value,as indicated by abnormal returns of stocks.Further,we tested the moderating effect of certain characteristic factors on abnormal stock returns.The research results illustrate a significant positive connection between SAPI announcements and shareholder value.Moreover,considering the announcement content and company factors,this study investigates the impacts of different investment targets and industries on the market reaction to SAPI announcements.We find that non-agricultural companies have a more positive market reaction to SAPI than agricultural companies;the higher the liability-asset ratio,the more positive will be the stock market reaction to SAPI. | Weihua Liu Shangsong Long Siyu Wang Ou Tang Jiahe Hou Jiahui Zhang | 2022 | Journal of Management Science and Engineering2022，7，3： | 0 |
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