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| 1 | Discriminated sgRNAs-Based SurroGate System Greatly Enhances the Screening Efficiency of Plant Base-Edited Cells显示文摘The development of CRISPR/Cas9-mediated base editing has made genomic modification more efficient. However, selection of genetically modified cells from millions of treated cells, especially plant cells, is still challenging. In this study, an efficient surrogate reporter system based on a defective hygromycin resistance gene was established in rice to enrich base-edited cells. After step-by-step optimization, the Discriminated sgRNAs-based SurroGate system (DisSUGs) was established by artificially differentiating the editing abilities of a wild-type single guide RNA (sgRNA) targeting the surrogate reporter gene and an enhanced sgRNA targeting endogenous sites. The DisSUGs enhanced the efficiency of screening base-edited cells by 3- to 5-fold for a PmCDA1-based cytosine-to-tyrosine base editor (PCBE), and 2.5- to 6.5-fold for an adenine base editor (ABE) at endogenous targets. These targets showed editing efficiencies of <25% in the conventional systems. The DisSUGs greatly enhanced the frequency of homozygous substitutions and expanded the activity window slightly for both a PCBE and an ABE. Analyses of the total number of single-nucleotide variants from whole-genome sequencing revealed that, compared with the no-enrichment PCBE strategy, the DisSUGs did not alter the frequency of genome-wide sgRNA-independent off-target mutations, but slightly increased the frequency of target-dependent off-target mutations. Collectively, the DisSUGs developed in this study greatly enhances the efficiency of screening plant base-edited cells and will be a useful system in future applications. | Wen Xu Yongxing Yang Ya Liu Guiting Kang Feipeng Wang Lu Li Xinxin Lv Si Zhao Shuang Yuan Jinling Song Ying Wu Feng Feng Xiaoqing He Chengwei Zhang Wei Song Jiuran Zhao Jinxiao Yang | 2020 | Molecular Plant2020，13，1： | 8 |
| 2 | Investigation and Analysis of Rabies Viral Infection and Distribution in China in 2005-2012显示文摘Dear Editor, We report the results of a preliminary investigation of data collected between 2005 and 2012. A National Human Rabies Surveillance program was initiated in high rabies incidence regions in 2005, and subsequently carried out nationally to monitor rabies situation. Our work presents a summary of epidemiological and etiologic rabies surveillance data collected since the implementation of the program. | Wentao Jiao Hao Li Xiaoyan Tao Miao Song Xinxin Shen Zhenyang Guo Yunjiao Zhao Qing Tang Guodong Liang | 2013 | Virologica Sinica2013，28，3： | 6 |
| 3 | Computer-aided ionic liquid design for alkane/cycloalkane extractive distillation process显示文摘A computer-aided ionic liquid design(CAILD) study is presented for the frequently encountered alkane/cycloalkane separations in petrochemical industry. Exhaustive experimental data are first collected to extend the UNIFAC-IL model for this system, where the proximity effect in alkanes and cycloalkanes is considered specifically by defining distinct groups. The thermodynamic performances of a large number of ILs for 4 different alkane/cycloalkane systems are then compared to select a representative example of such separations. By applying n-heptane/methylcyclohexane extractive distillation as a case study, the CAILD task is cast as a mixed-integer nonlinear programming(MINLP) problem based on the obtained task-specific UNIFAC-IL model and two semi-empirical models for IL physical properties. The top 5 IL candidates determined by solving the MINLP problem are subsequently introduced into Aspen Plus for process simulation and economic analysis, which finally identify 1-hexadecyl-methylpiperidinium tricyanomethane([C_(16)MPip][C(CN)_3]) as the best entrainer for this separation. | Zhen Song Xinxin Li He Chao Fan Mo Teng Zhou Hongye Cheng Lifang Chen Zhiwen Qi | 2019 | Green Energy & Environment2019，4，2： | 6 |
| 4 | Research Progress of Cellulase显示文摘Cellulase is a complex enzyme that can decompose cellulose into glucose,and it could effectively treat cellulose waste. In this paper,it aims to explore development status and research progress of cellulase,and introduce concept and action mechanism of cellulase,research situation of cellulase in molecular aspect,application of cellulase,and development of cellulase is also prospected. | Lili SONG Xinxin LIU | 2019 | Asian Agricultural Research2019，11，3： | 5 |
| 5 | Metal-support interactions in designing noble metal-based catalysts for electrochemical CO_(2) reduction: Recent advances and future perspectives显示文摘Electrochemical CO_(2) reduction reaction (CO_(2) RR) offers a practical solution to current global greenhouse effect by converting excessive CO_(2) into value-added chemicals or fuels. Noble metal-based nanomaterials have been considered as efficient catalysts for the CO_(2) RR owing to their high catalytic activity, long-term stability and superior selectivity to targeted products. On the other hand, they are usually loaded on different support materials in order to minimize their usage and maximize the utilization because of high price and limited reserve. The strong metal-support interaction (MSI) between the metal and substrate plays an important role in affecting the CO_(2) RR performance. In this review, we mainly focus on different types of support materials (e.g., oxides, carbons, ligands, alloys and metal carbides) interacting with noble metal as electrocatalysts for CO_(2) RR. Moreover, the positive effects about MSI for boosting the CO_(2) RR performance via regulating the adsorption strength, electronic structure, coordination environment and binding energy are presented. Lastly, emerging challenges and future opportunities on noble metal electrocatalysts with strong MSI are discussed. | Zhao Li Rui Wu Lei Zhao Pingbo Li Xinxin Wei Junjie Wang Jun Song Chen Tierui Zhang | 2021 | Nano Research2021，14，11： | 4 |
| 6 | Diversity of Sex Types and Seasonal Sexual Plasticity in a Cucumber Germplasm Collection显示文摘The sex type of a cucumber plant is determined by the proportion of male, female and hermaphrodite flowers that it bears and is an important factor that affects fruit yield. In this paper, the sex types and seasonal sexual stabilities of 322 accessions of cucumber germplasm were identified. This germplasm collection displayed a great variety of sex types. We used an updated 10-type sex classification system based on the flower types present and the proportion of nodes with pistillate flowers(PNPF). The PNPF ranges of all the accessions were 2.12%–100% in spring and 0–100% in autumn. A total of 81.37% of the accessions had PNPFs of 10%–50% in spring, but most(84.78%) accessions were reduced to 0–20% PNPF in autumn. The range of reduction of PNPF from spring to autumn was 0–67.91%. In other words, most of the germplasm was normal monoecious(31.68%) or subandroecious(62.73%) in spring, but 94.10% of the accessions were subandroecious in autumn. According to the statistical evaluation of the difference in PNPFs between the two seasons, each accession could be classified into one of three groups: seasonally stable, seasonally sensitive and highly seasonally sensitive, accounting for 10.56%, 20.50% and 68.94% of the accessions, respectively. With a few exceptions, the seasonal PNPF differences were positively correlated with the PNPFs in a given season for most accessions. These results provided useful information and materials for sex expression mechanism research and for breeding cucumbers with high and stable yields. | DOU Xinxin SHEN Di ZHANG Xiaohui CHENG Jiaqi LI Sigeng SONG Jiangping WANG Haiping ZHANG Wei LI Xixiang | 2015 | Horticultural Plant Journal2015，1，2： | 3 |
| 7 | Hyperthermia based individual in situ recombinant vaccine enhances lymph nodes drainage for de novo antitumor immunity显示文摘The continuing challenges that limit effectiveness of tumor therapeutic vaccines were high heterogeneity of tumor immunogenicity, low bioactivity of antigens, as well as insufficient lymph nodes(LNs) drainage of antigens and adjuvants. Transportation of in situ neoantigens and adjuvants to LNs may be an effective approach to solve the abovementioned problems. Therefore, an FA-TSL/AuNCs/SV nanoplatform was constructed by integrating simvastatin(SV) adjuvant loaded Au nanocages(AuNCs)as cores(AuNCs/SV) and folic acid modified thermal-sensitive liposomes(FA-TSL) as shells to enhance de novo antitumor immunity. After accumulation in tumor guided by FA, AuNCs mediated photothermal therapy(PTT) induced the release of tumor-derived protein antigens(TDPAs) and the shedding of FATSL. Exposed AuNCs/SV soon captured TDPAs to form in situ recombinant vaccine(AuNCs/SV/TDPAs). Subsequently, AuNCs/SV/TDPAs could efficiently transport to draining LNs owing to the hyperthermia induced vasodilation effect and small particle size, achieving co-delivery of antigens and adjuvant for initiation of specific T cell response. In melanoma bearing mice, FA-TSL/AuNCs/SV and laser irradiation effectively ablated primary tumor, against metastatic tumors and induced immunological memory. This approach served a hyperthermia enhanced platform drainage to enable robust personalized cancer vaccination. | Cuixia Zheng Xinxin Liu Yueyue Kong Lei Zhang Qingling Song Hongjuan Zhao Lu Han Jiannan Jiao Qianhua Feng Lei Wang | 2022 | Acta Pharmaceutica Sinica B2022，12，8： | 2 |
| 8 | Density-functional-theory predictions of mechanical behaviour and thermal properties as well as experimental hardness of the Ga-bilayer Mo_(2)Ga_(2)C显示文摘Mo_(2)Ga_(2)C is a new MAX phase with a stacking Ga-bilayer as well as possible unusual properties.To understand this unique MAX phase structure and promote possible future applications,the structure,chemical bonding,and mechanical and thermodynamic properties of Mo_(2)Ga_(2)C were investigated by first-principles.Using the“bond stiffness”model,the strongest covalent bonding(1162 GPa)was formed between Mo and C atoms in Mo_(2)Ga_(2)C,while the weakest Ga–Ga(389 GPa)bonding was formed between two Ga-atomic layers,different from other typical MAX phases.The ratio of the bond stiffness of the weakest bond to the strongest bond(0.33)was lower than 1/2,indicating the high damage tolerance and fracture toughness of Mo_(2)Ga_(2)C,which was confirmed by indentation without any cracks.The high-temperature heat capacity and thermal expansion of Mo_(2)Ga_(2)C were calculated in the framework of quasi-harmonic approximation from 0 to 1300 K.Because of the metal-like electronic structure,the electronic excitation contribution became more significant with increasing temperature above 300 K. | Xinxin QI Weilong YIN Sen JIN Aiguo ZHOU Xiaodong HE Guangping SONG Yongting ZHENG Yuelei BAI | 2022 | Journal of Advanced Ceramics2022，11，2： | 2 |
| 9 | Amelioration of imiquimod-induced psoriasis-like dermatitis in mice by DSW therapy inspired hydrogel显示文摘Psoriasis is a long-lasting and recurrent autoimmune disease which is incurable so far.Dead Sea water(DSW)therapy is an effective approach to help control the symptoms of psoriasis due to the abundant mineral ions in DSW,which inspired the material formulation in this study.Rubidium–Sodium alginate/Polyacrylamide hydrogels(Rb-SA/PAAm gels)composed of sodium alginate and polyacrylamide interpenetrating network structure with different concentrations of rubidium and certain magnesium and zinc content were prepared for the treatment of psoriasis.The obtained results suggest the good mechanical properties of the Rb-SA/PAAm gels including toughness and swelling performance.In terms of in vitro tests,the Rb-SA/PAAm gels not only show nontoxicity to human keratinocyte cell line(Hacats)but also inhibits the activity against inflammatory NF-κβ signaling pathway.Meanwhile,they can release Rb+which enable the Rb-SA/PAAm gels have better antibacterial ability to Streptococcus and Escherichia coli.The results obtained from in vivo tests indicate that these hydrogels could alleviate the symptoms of psoriasis caused by Imiquimod(IMQ)in mice by reducing the inflammatory factor in STAT3 pathway and therefore reduce the immune stimulation of the spleen.In conclusion,the 100Rb-SA/PAAm gel has demonstrated great potential to be a topical wettable dressing for psoriasis treatment. | Xiang He Bing Zhu WeiJia Xie Yu He Jian Song Yi Zhang Chi Sun Hao Li QiYu Tang XinXin Sun Yanni Tan Yong Liu | 2021 | Bioactive Materials2021，6，2： | 2 |
| 10 | Highly Efficient Adsorption of P-Xylene from Aqueous Solutions by Hierarchical Nanoporous Biochar Derived from Crab Shell显示文摘The global consumption of p-xylene(PX)for the production of polymers has raised serious concerns about its impact on the environment.As various reports have shown the risks that PX could pose to human health,research into cost-effective remedial methods to remove PX from the environment has gained attraction.In this work,a hierarchical porous crab shell biochar(KCS)was synthesized,characterized,and evaluated for its efficiency to remove PX from aqueous solution.The characterizations of KCS,including the porous structure,surface functional group,phase structure,and surface morphology,were discussed by N_(2) adsorption-desorption,FTIR,XRD,and SEM.Batch adsorption experiments showed that the maximum adsorption capacity of PX on KCS was 393 mg/g within 5 min,larger than most biological/biomass materials,mainly due to the higher specific surface area of 2046 m^(2)/g,and abundant lipophilic functional groups.Subsequent adsorption kinetics study indicated a pseudo-second-order model which implied that the adsorption of PX was due to chemisorption.Thermodynamic parameters showed that the values ofΔH°andΔG°were both negative,indicating that the PX adsorption process on KCS was spontaneous and exothermic.The performance of KCS in delivering a cost-effective,fast,and efficient solution for the removal of PX from aqueous solution would greatly benefit current environmental remediation efforts. | Yingna Chen Hailong Zhang Jian Guo Lu Cai Yaning Wang Xinxin Yao Wendong Song Lili Ji | 2021 | Journal of Renewable Materials2021，9，10： | 1 |
| 11 | Mi- cro - cantilever Shocking - acceleration Switches with Threshold Adjusting and 'On' -state Latching Functions显示文摘 | Jia Mengjun Li Xinxin Song Zhaohui | 2007 | Journal of Micromechanics and Microengineering2007，17，3： | 1 |
| 12 | Orchestrating antigen delivery and presentation efficiency in lymph node by nanoparticle shape for immune response显示文摘Activating humoral and cellular immunity in lymph nodes(LNs)of nanoparticle-based vaccines is critical to controlling tumors.However,how the physical properties of nanovaccine carriers orchestrate antigen capture,lymphatic delivery,antigen presentation and immune response in LNs is largely unclear.Here,we manufactured gold nanoparticles(AuNPs)with the same size but different shapes(cages,rods,and stars),and loaded tumor antigen as nanovaccines to explore their disparate characters on above four areas.Results revealed that star-shaped AuNPs captured and retained more repetitive antigen epitopes.On lymphatic delivery,both rods and star-shaped nanovaccines mainly drain into the LN follicles region while cage-shaped showed stronger paracortex retention.A surprising finding is that the star-shaped nanovaccines elicited potent humoral immunity,which is mediated by CD4^(+)T helper cell and follicle B cell cooperation significantly preventing tumor growth in the prophylactic study.Interestingly,cage-shaped nanovaccines preferentially presented peptide-MHC I complexes to evoke robust CD8^(+)T cell immunity and showed the strongest therapeutic efficacy when combined with the PD-1 checkpoint inhibitor in established tumor study.These results highlight the importance of nanoparticle shape on antigen delivery and presentation for immune response in LNs,and our findings support the notion that different design strategies are required for prophylactic and therapeutic vaccines. | Hongjuan Zhao Yatong Li Beibei Zhao Cuixia Zheng Mengya Niu Qingling Song Xinxin Liu Qianhua Feng Zhenzhong Zhang Lei Wang | 2023 | Acta Pharmaceutica Sinica B2023，13，9： | 1 |
| 13 | Gas discharge in a gas peaking switch显示文摘 | Wang Xinxin Hu Yuan Song Xinhai | 2005 | Laser and Particle Beams2005，23，4： | 1 |
| 14 | Aromatic plants play an important role in promoting soil biologicalactivity related to nitrogen cycling in an orchard ecosystem显示文摘 | Chen Xinxin Song Beizhou Yao Yuncong | 2014 | Science of the Total Environment2014，，472： | 1 |
| 15 | Current progress and prospects of enzyme technologies in future foods显示文摘Enzyme technologies are widely used in the food industry due to their advantages of high efficiency,specificity,and safety.Recently,“future foods”is emerging as a new research hotspot with healthier foods that are more nutritious,delicious,and sustainable;however,these foods still have problems with texture,nutrition,and flavor.Advances in enzyme technology have enabled the development of new tools and approaches to better manipulate food textures and nutritional aspects.In this review,we summarize enzyme technology applications in future food production,focusing on food texture,safety,and flavors.Furthermore,we discuss the prospects of enzyme-based technologies for future food production,including the modification of enzyme activities,the development of suitable food-grade hosts for enzyme production,and the optimiza-tion synergistic multi-enzyme systems. | Cuiping Pang Xinxin Yin Guoqiang Zhang Song Liu Jingwen Zhou Jianghua Li Guocheng Du | 2021 | Systems Microbiology and Biomanufacturing2021，1，1： | 1 |
| 16 | A high-performance micro-machined piezoresistive accelerometer with axially stressed tiny beams 显示文摘 | Huang Shusen Li Xinxin Song Zhanhui | 2001 | Journal of Micro-machines and Micro-engineering2001，15，3： | 1 |
| 17 | Impact of the PV Location in Distribution Networks on Network Power Losses and Voltage Fluctuations with PSO Analysis显示文摘Increased grid integration of photovoltaic(PV)has aggravated the uncertainty of distribution network operations.For a distribution network with PV,the impact of the PV location on the network power losses and voltage fluctuations is investigated with analytical derivations reflected by the line impedance.Optimization approaches of the PV location with consideration of two aspects,i.e.,minimum network power losses and minimum voltage fluctuations,are analyzed.A particle swarm optimization(PSO)algorithm is used to synthesize an optimal compromised solution so as to determine the PV location.A 10 kV distribution network with one PV is established on the time-domain simulation environment PSCAD/EMTDC.The simulation results justify the theoretical analysis and indicate that when the active power of the PV is more/less than twice that of the overall loads/end loads,the network power losses and node voltage fluctuations are both minimum when the PV is integrated into the head/tail end of the network.When the active power of the PV is between the above two conditions,nodes t/f can be identified for the integration of the PV between the head/end nodes of the network to achieve the minimum network power losses/voltage fluctuations,respectively.The effectiveness of the proposed optimization approach is verified and can provide a reference for selecting the PV location in the distribution network. | Puyu Wang Fangyu Liang Jinyuan Song Ningqiang Jiang Xiao-Ping Zhang Ling Guo Xinxin Gu | 2022 | CSEE Journal of Power and Energy Systems2022，8，2： | 1 |
| 18 | Changes in Several Physiological and Biochemical Indices of Maize Seedling Roots Caused by Drought Stress显示文摘The changes in several physiological and biochemical indices of seedling roots of new maize variety Qingnong 8 were studied under the simulated drought condition with 18% PEG-6000 and water shortage. The results showed that under drought conditions,the contents of soluble protein and malonialdehy( MDA) and the activities of superoxide dismutase( SOD) and peroxidase( POD) in roots of maize seedlings significantly increased,and the increasing amplitude reduced after water shortage for 96 h. The re-watering treatment results after 48 h water shortage showed that the SOD and POD activities and the MDA content could recover to normal level,and the soluble protein content was lower than normal content. This study showed that the maize seedlings of Qingnong 8 suffered drought injury could grow normally after re-watering treatment. | Cui Limei Wang Xinxin Xuan Huidong Song Xiyun Pei Yuhe | 2015 | Plant Diseases and Pests2015，6，4： | 1 |
| 19 | Gas discharge in a gas peaking switch显示文摘 | Xinxin Wang Yuan Hu Xinhai Song | 2005 | Laser and Particle Beams2005，23，4： | 1 |
| 20 | Health risk assessment on dietary exposure to polycyclic aromatic hydrocarbons (PAHs) in Taiyuan, China显示文摘 | Zhonghuan Xia Xiaoli Duan Weixun Qiu Di Liu Bin Wang Shu Tao Qiujing Jiang Bin Lu Yunxue Song Xinxin Hu | 2010 | Science of the Total Environment2010，，22： | 1 |
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