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| 1 | GSDMB promotes non-canonical pyroptosis by enhancing caspase-4 activity显示文摘Gasdermin B (GSDMB) has been reported to be associated with immune diseases in humans, but the detailed molecular mechanisms remain unsolved. The N-terminus of GSDMB by itself, unlike other gasdermin family proteins, does not induce cell death. Here, we show that GSDMB is highly expressed in the leukocytes of septic shock patients, which is associated with increased release of the gasdermin D (GSDMD) N-terminus. GSDMB expression and the accumulation of the N-terminal fragment of GSDMD are induced by the activation of the non-canonical pyroptosis pathway in a human monocyte cell line. The downregulation of GSDMB alleviates the cleavage of GSDMD and cell death. Consistently, the overexpression of GSDMB promotes GSDMD cleavage, accompanied by increased LDH release. We further found that GSDMB promotes caspase-4 activity, which is required for the cleavage of GSDMD in non-canonical pyroptosis, by directly binding to the CARD domain of caspase-4. Our study reveals a GSDMB-mediated novel regulatory mechanism for non-canonical pyroptosis and suggests a potential new strategy for the treatment of inflammatory diseases. | Qin Chen Peiliang Shi Yufang Wang Dayuan Zou Xiuwen Wu Dingyu Wang Qiongyuan Hu Yujie Zou Zan Huang Jianan Ren Zhaoyu Lin Xiang Gao | 2019 | Journal of Molecular Cell Biology2019，11，6： | 22 |
| 2 | Microfluidic Electrospray Niacin Metal-Organic Frameworks Encapsulated Microcapsules for Wound Healing显示文摘Niacin metal-organic frameworks(MOFs)encapsulated microcapsules with alginate shells and copper-/zinc-niacin framework cores were in situ synthesized by using a microfuidic electrospray approach for wound healing.As the alginate shells were bacteriaresponsively degradable,the niacin MOFs encapsulated microcapsules could intelligently,controllably,and programmably release calcium,copper,and zinc ions,depending on the degree of infections.Te released ions could not only kill microbes by destroying their membrane and inducing the outfow of nutrient substance,but also activate copper/zinc superoxide dismutase(Cu/ZnSOD)to eliminate oxygen free radicals and rescue the cells from oxidative stress injury.Furthermore,the simultaneously released niacin could promote hemangiectasis and absorption of functional metal ions.Tus,the niacin MOFs encapsulated microcapsules were imparted with outstanding antibacterial,antioxidant,and angiogenesis properties.Based on an in vivo study,we have also demonstrated that the chronic wound healing process of an infected full-thickness skin defect model could be signifcantly enhanced by using the niacin MOFs encapsulated microcapsules as therapeutic agent.Terefore,the microfuidic electrospray niacin MOFs encapsulated microcapsules are potential for clinical applications. | Guopu Chen Yunru Yu Xiuwen Wu Gefei Wang Guosheng Gu Feng Wang Jianan Ren Huidan Zhang Yuanjin Zhao | 2019 | Research2019，，1： | 21 |
| 3 | The prognostic and therapeutic implications of circulating tumor cell phenotype detection based on epithelial-mesenchymal transition markers in the first-line chemotherapy of HER2-negative metastatic breast cancer显示文摘Background:Epithelial-mesenchymal transition(EMT)is implicated in the metastatic process and presents a chal-lenge to epithelial cell adhesion molecule-based detection of circulating tumor cells(CTCs),which have been demon-strated to be a prognostic indicator in metastatic breast cancer.Although evidence has indicated that heterogeneity of CTCs based on EMT markers is associated with disease progression,no standard recommendations have been established for clinical practice.This study aimed to evaluate the prognostic significance of dynamic CTC detection based on EMT for metastatic breast cancer patients.Methods:We enrolled 108 human epidermal growth factor receptor 2-negative metastatic breast cancer patients from the prospective phase III CAMELLIA study and applied the CanPatrol CTC enrichment technique to identify CTC phenotypes(including epithelial CTCs,biphenotypic epithelial/mesenchymal CTCs,and mesenchymal CTCs)in peripheral blood samples.Receiver operating characteristic curve analyses of total CTC count and the proportion of mesenchymal CTCs for predicting the 1-year progression-free survival(PFS)rate were conducted to determine the optimal cut-off values,and Kaplan-Meier analysis and Cox proportional hazards regression analysis were performed to investigate the prognostic value of the cut-off values of both total CTC count and the proportion of mesenchymal CTCs in combination.Results:For predicting the 1-year PFS rate,the optimal cut-off value of total CTC count was 9.5(Area under the curve[AUC]=0.538,95%confidence interval[CI]=0.418-0.657),and that of the proportion of mesenchymal CTCs was 10.7%(AUC=0.581,95%CI=0.463-0.699).We used the two cut-off values in combination to forecast PFS in which the total CTC count was equaled to or exceeded 10/5 mL with the proportion of mesenchymal CTCs surpassed 10.7%.Patients who met the combined criteria had significantly shorter median PFS than did those who did not meet the criteria(6.2 vs.9.9 months,P=0.010).A nomogram was constructed based on the criteria and significant clinicopatho-logical characteristics with a C-index of 0.613(P=0.010).Conclusions: The criteria, which combine the total CTC count and the proportion of mesenchymal CTCs, may be used to monitor therapeutic resistance and predict prognosis in patients with metastatic breast cancer. | Xiuwen Guan Fei Ma Chunxiao Li Shiyang Wu Shangying Hu Jiefen Huang Xiaoying Sun Jiayu Wang Yang Luo Ruigang Cai Ying Fan Qiao Li Shanshan Chen Pin Zhang Qing Li Binghe Xu | 2019 | Cancer Communications2019，39，1： | 15 |
| 4 | Biomimetic intestinal barrier based on microfluidic encapsulated sucralfate microcapsules显示文摘Intestinal barriers play an important role in preventing intestinally derived diseases,and maintaining their function is a promising approach to prevent and treat those diseases.Here,inspired by the protection effect of intestinal barriers in live organisms and the mucosa adhesive property of sucralfate,we present a biomimetic intestinal barrier based on microfluidic encapsulated sucralfate microcapsules.Benefiting from the flexible selectivity and precise control of microfluidic electrospray flows,the generated microcapsules were imparted with stomach-tolerant dietary-fibre shells and controllable released sucralfate cores,both of which could contribute to forming a continuous biomimetic intestinal barrier on the intestine.Through in vitro adhesive study,in vivo computed tomography(CT)imaging and in vivo imaging system(IVIS)methods,we have demonstrated that the microcapsule-derived biomimetic intestinal barrier can effectively block food fermentation in the gut,reduce generation of fat,decrease disease risk indexes,and prevent obesity.These features make the microfluidic encapsulated sucralfate microcapsules and their resultant biomimetic intestinal barrier an approach for treating obesity and other intestinal diseases. | Cheng Zhao Yunru Yu Xiaoxuan Zhang Xiuwen Wu Jianan Ren Yuanjin Zhao | 2019 | Science Bulletin2019，64，19： | 4 |
| 5 | Equivalent efficacy study of QL1101 and bevacizumab on untreated advanced non-squamous non-small cell lung cancer patients: a phase 3 randomized, double-blind clinical trial显示文摘Objective:This phase 3 study aimed to test equivalence in efficacy and safety for QL1101,a bevacizumab analogue in Chinese patients with untreated locally advanced non-squamous non-small cell lung cancer(NSCLC).Methods:Eligible patients were randomly assigned 1:1 to receive carboplatin and paclitaxel in combination with either QL1101 or bevacizumab,15 mg/kg every 3-week for 6 cycles.This was followed by maintenance treatment with single agent QL1101 every 3-week.The primary end-point was objective response rate(ORR),with secondary end-points being progression-free survival(PFS),overall survival(OS),disease control rate(DCR),and adverse events(AEs).Results:Of 675 patients,535 eligible patients were randomized to the QL1101 group(n=269)and bevacizumab group(n=266).ORRs were 52.8%and 56.8%,respectively,for the QL1101 and bevacizumab groups,with an ORR hazard ratio 0.93(95%confidence interval:0.8-0131.1).The PFS,OS,DCR,and AEs were comparable between the 2 groups,which remained the same after stratification according to epidermal growth factor receptor mutation or smoking history.Conclusions:QL1101 showed similar efficacy and safety profiles as compared to bevacizumab among Chinese patients with untreated locally advanced non-squamous NSCLC. | Tianqing Chu Jun Lu Minghong Bi Helong Zhang Wu Zhuang Yan Yu Jianhua Shi Zhendong Chen Xiaochun Zhang Qisen Guo Quan Liu Huijuan Wu Jian Fang Yi Hu Xiuwen Wang Cuicui Han Kai Li Baohui Han | 2021 | Cancer Biology & Medicine2021，18，3： | 4 |
| 6 | Biocompatibility, biodegradation, and neovascularization of human single-unit platelet-rich fibrin glue: an in vivo analysis显示文摘 | Wu Xiuwen Ren Jianan Yao Genhong Zhou Bo Wang Gefei Gu Guosheng Luan Jianfeng | 2014 | Chinese Medical Journal2014，，3： | 3 |
| 7 | Marine-inspired molecular mimicry generates a drug-free, but immunogenic hydrogel adhesive protecting surgical anastomosis显示文摘Herein,we report the synthesis of a biomimic hydrogel adhesive that addresses the poor healing of surgical anastomosis.Dopamine-conjugated xanthan gum(Da-g-Xan)is fabricated using deep insights into the molecular similarity between mussels'adhesive and dopamine as well as the structural similarity between barnacle cement proteins and xanthan gum.The hydrogel mimics marine animals’adherence to wet tissue surfaces.Upon applying this adhesive to colonic anastomosis in a rat model,protective effects were shown by significantly improving the bursting pressure.Mechanistically,the architecture of Da-g-Xan hydrogel is maintained by dynamic intermolecular hydrogen bonds that allow the quick release of Da-g-Xan.The free Da-g-Xan can regulate the inflammatory status and induce type 2 macrophage polarization(M2)by specifically interacting with mannose receptors(CD206)revealed by RNA-sequencing and molecular binding assays.Consequently,an appropriate microenvironment for tissue healing is created by the secretion of chemokines and growth factors from M2 macrophages,strengthening the fibroblast migration and proliferation,collagen synthesis and epithelial vascularization.Overall,this study demonstrates an unprecedented strategy for generating an adhesive by synergistic mimicry inspired by two marine animals,and the results show that the Da-g-Xan adhesive augments native tissue regenerative responses,thus enabling enhanced recovery following surgical anastomosis. | Jinjian Huang Yungang Jiang Ye Liu Yanhan Ren Ziyan Xu Zongan Li Yun Zhao Xiuwen Wu Jianan Ren | 2021 | Bioactive Materials2021，6，3： | 2 |
| 8 | Sulfur-free exfoliated graphite with large exfoliated volume:preparation, characterization and its adsorption performance 显示文摘 | ZHAO Qi CHENG Xiuwen WU Juan | 2014 | Journal of Industrial and Engineering Chemistry2014，20，6： | 1 |
| 9 | Explo- ring texture ensembles by efficient markov chain monte carlo-toward a 'trichromacy' theory of texture 显示文摘 | Zhu Songchun Liu Xiuwen Wu Yyingnian | 2000 | IEEE Transactions on Pattern Analysis and Machine Intelligence2000，22，6： | 1 |
| 10 | Preparation of cement water re-ducer of modified lignosulfonate显示文摘 | Wu Xiuwen Zhang Heng | 2011 | Advanced MaterialsResearch2011，168，： | 1 |
| 11 | Treatment of wastewater con-taining EDTA-Cu(Ⅱ) using the combined process of interior mi-croelectrolysis and Fenton oxidation-coagulation显示文摘 | Lan Shanhong Ju Feng Wu Xiuwen | 2012 | Separation and Purification Technology2012，89，1： | 1 |
| 12 | C rohn’s disease and polymorphism of heat shock protein gene HSP 70‐2 in the C hinese population显示文摘 | Jun Chen Jianan Ren Guosheng Gu Gefei Wang Xiuwen Wu Dongsheng Yan Song Liu Jieshou Li | 2013 | J Gastroenterol Hepatol2013，，5： | 1 |
| 13 | 基于上皮–间质转化标志物的循环肿瘤细胞表型检测对HER2阴性转移性乳腺癌一线化疗的预后意义显示文摘背景与目的上皮–间质转化(epithelia-mesenchymal,EMT)参与肿瘤细胞的转移,并且对基于上皮细胞黏附分子的循环肿瘤细胞(circulating tumor cells,CTCs)检测技术提出了挑战,CTCs已被证实是转移性乳腺癌的预后指标。虽然有证据表明基于EMT标志物的CTCs异质性与疾病进展相关,但在临床应用方面尚无统一的参考标准。本研究旨在评估基于EMT的动态CTCs检测对转移性乳腺癌患者的预后意义。方法我们从CAMELLIA III期前瞻性研究中招募了108例人表皮生长因子受体2阴性转移性乳腺癌患者,并应用CanPatrol CTC富集技术检测外周血中不同亚型的CTCs(包括上皮型CTCs、混合型上皮/间质CTCs和间质型CTCs)。应用1年无进展生存(progression-free survival,PFS)率作为界值分别寻找CTC总数和间质CTCs比例的受试者工作曲线来确定最佳阈值,并采用Kaplan-Meier分析和Cox比例风险回归分析验证综合考虑CTC总数和间质型CTCs比例制定的诊断标准的预后价值。结果CTC总数的最佳阈值为9.5[曲线下面积(area under the curve,AUC)=0.538,95%置信区间(confidence interval,CI=0.418–0.657)]及间质CTCs比例为10.7%(AUC=0.581,95%CI:0.463–0.699)作为预测1年PFS率的界值。我们采用CTC总数≥10/5 mL且间质CTCs比例>10.7%的联合标准预测PFS。符合联合标准的患者的中位PFS显著短于不符合标准的患者(6.2个月vs.9.9个月,P=0.010)。根据该标准和显著的临床病理学特征绘制列线图,C指数为0.613(P=0.010)。结论CTC总数和间质CTCs比例的联合标准可用于监测转移性乳腺癌患者的治疗耐药性和预测患者预后。 | Xiuwen Guan Fei Ma Chunxiao Li Shiyang Wu Shangying Hu Jiefen Huang Xiaoying Sun Jiayu Wang Yang Luo Ruigang Cai Ying Fan Qiao Li Shanshan Chen Pin Zhang Qing Li Binghe Xu | 2019 | 癌症2019，38，9： | 1 |
| 14 | Recombinant human thrombopoietin improves platelet counts and reduces platelet transfusion possibility among severe sepsis patients with thrombocytopenia: A prospective study显示文摘 | Qin Wu Jianan Ren Xiuwen Wu Gefei Wang Guosheng Gu Song Liu Yin Wu Dong Hu Yunzhao Zhao Jieshou Li | 2013 | Journal of Critical Care2013，，： | 1 |
| 15 | T2 enhances in situ level of Foxp3 + regulatory cells and modulates inflammatory cytokines in Crohn’s disease显示文摘 | Guanwei Li Jianan Ren Gefei Wang Guosheng Gu Dong Hu Huajian Ren Zhiwu Hong Xiuwen Wu Song Liu Jieshou Li | 2013 | International Immunopharmacology2013，，： | 1 |
| 16 | Geographic Mapping of Crohn’s Disease and Its Relation to Affluence in Jiangsu Province, an Eastern Coastal Province of China显示文摘 | Dong Hu Jianan Ren Gefei Wang Guosheng Gu Song Liu Xiuwen Wu Jun Chen Huajian Ren Zhiwu Hong Jieshou Li Paul Henderson | 2014 | Gastroenterology Research and Practice2014，，： | 1 |
| 17 | Equivalence of Julesz Ensembles and FRAME Models显示文摘 | Ying Nian Wu Song Chun Zhu Xiuwen Liu | 2000 | International Journal of Computer Vision2000，，3： | 1 |
| 18 | Equivalence of Julesz Ensembles and FRAME Models显示文摘 | Ying Nian Wu Song Chun Zhu Xiuwen Liu | 2000 | International Journal of Computer Vision2000，，3： | 1 |
| 19 | Treatment of wastewater co- ntaining EDTA-Cu ( I1 ) using the combined process of interior mi- eroeleetrolysis and Fenton oxidation-coagulation 显示文摘 | Lan Shanhong Ju Feng Wu Xiuwen | 2012 | Separation andPurification Technology2012，89，： | 1 |
| 20 | Microfluidic intestinal organoid-on-a-chip uncovers therapeutic targets by recapitulating oxygen dynamics of intestinal IR injury显示文摘Increasing evidence demonstrates that mammals have different reactions to hypoxia with varied oxygen dynamic patterns.It takes~24 h for tri-gas incubator to achieve steady cell hypoxia,which fails to recapitulate ultrafast oxygen dynamics of intestinal ischemia/reperfusion(IR)injury.Inspired from the structure of native intestinal villi,we engineered an intestinal organoid chip embedded with engineered artificial microvessels based on coaxial microfluidic technology by using pH-responsive ZIF-8/sodium alginate scaffold.The chip was featured on:(i)eight times the oxygen exchange efficiency compared with the conventional device,tri-gas incubator,(ii)implantation of intestinal organoid reproducing all types of intestinal epithelial cells,and(iii)bio-responsiveness to hypoxia and reoxygenation(HR)by presenting metabolism disorder,inflammatory reaction,and cell apoptosis.Strikingly,it was found for the first time that Olfactomedin 4(Olfm4)was the most significantly downregulated gene under a rapid HR condition by sequencing the RNA from the organoids.Mechanistically,OLFM4 played protective functions on HR-induced cell inflammation and tissue damage by inhibiting the NF-kappa B signaling activation,thus it could be used as a therapeutic target.Altogether,this study overcomes the issue of mismatched oxygen dynamics between in vitro and in vivo,and sets an example of next-generation multisysteminteractive organoid chip for finding precise therapeutic targets of IR injury. | Jinjian Huang Ziyan Xu Jiao Jiao Zongan Li Sicheng Li Ye Liu Ze Li Guiwen Qu Jie Wu Yun Zhao Kang Chen Jieshou Li Yichang Pan Xiuwen Wu Jianan Ren | 2023 | Bioactive Materials2023，，12： | 0 |
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