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| 1 | Characterization of microRNAs in serum: a novel class of biomarkers for diagnosis of cancer and other diseases显示文摘在各种各样的纸巾的 microRNAs (miRNAs ) 的 Dysregulated 表示与许多疾病被联系了,包括癌症。这里,我们证明 miRNAs 在象老鼠,老鼠,牛的胎儿,小牛,和马那样的人和另外的动物的浆液和血浆是在场的。在浆液的 miRNAs 的层次在一样的种类的个人之中稳定、可再现、一致。采用 Solexa,我们定序健康中国题目的所有浆液 miRNAs 并且分别地在男、女的题目发现超过 100 和 91 浆液 miRNAs。我们也为肺癌症, colorectal 癌症,和糖尿病识别了浆液 miRNAs 的特定的表示模式,提供证据那浆液 miRNAs 为各种各样的疾病包含指纹。癌症特定的浆液 miRNAs 由 Solexa 获得了的二个非小的房间肺进一步在 75 个健康施主和 152 个癌症病人的独立审判被验证,用量的反向的抄写聚合酶链反应试金。通过这些分析,我们断定浆液 miRNAs 能为各种各样的癌症和另外的疾病的察觉用作潜在的 biomarkers。 | Xi Chen Yi Ba Lijia Ma Xing Cai Yuan Yin Kehui Wang Jig ang Guo Yujing Zhang Jiangning Chen Xing Guo Qibin Li Xiaoying Li Wenjing Wang Yan Zhang Jin Wang Xueyuan Jiang Yang Xiang Chen Xu Pingping Zheng Juanbin Zhang Ruiqiang Li Hongjie Zhang Xiaobin Shang Ting Gong Guang Ning Jun Wang Ke Zen Junfeng Zhang Chen-Yu Zhang | 2008 | Cell Research2008，18，10： | 975 |
| 2 | Exogenous plant MIR168a specifically targets mammalian LDLRAPI: evidence of cross-kingdom regulation by microRNA显示文摘 | Lin Zhang Dongxia Hou Xi Chen Donghai Li Lingyun Zhu Yuj ing Zhang Jing Li Zhen Bian Xiangying Liang Xing Cai Yuan Yin Cheng Wang Tianfu Zhang Dihan Zhu Dianmu Zhang Jie Xu Qun Chen Yi Ba Jing Liu Qiang Wang Jianqun Chen Jin Wang Meng Wang Qipeng Zhang Junfeng Zhang Ke Zen Chen-Yu Zhang | 2012 | Cell Research2012，22，1： | 155 |
| 3 | Heavy metal pollution in intertidal sediments from Quanzhou Bay, China显示文摘The concentrations of eight heavy metals (Cu, Zn, Pb, Cd, Cr, Ni, Hg, and As) in the intertidal surface sediments from Quanzhou Bay were determined to evaluate their levels and spatial distribution due to urbanization and economic development of Quanzhou region, southeast China. The ranges of the measured concentrations in the sediments are as follows: 24.8–119.7 mg/kg for Cu, 105.5–241.9 mg/kg for Zn, 34.3–100.9 mg/kg for Pb, 0.28–0.89 mg/kg for Cd, 51.1–121.7 mg/kg for Cr, 16.1–45.7 mg/kg for Ni, 0.17–0.74 mg/kg for Hg, and 17.7–30.2 mg/kg for As. The overall average concentrations of above metals exceed the primary standard criteria but meet the secondary standard criteria of the Chinese National Standard of Marine Sediment Quality. Several contents of Cu and Hg exceed the secondary standard criteria at some stations. The results of geoaccumulation index (Igeo) show that Cd causes strong pollution in most of the study area. There are no significant correlations among most of these heavy metals, indicating they have different anthropogenic and natural sources. Some locations present severe pollution by heavy metals depending on the sources, of which sewage outlets, aquatic breeding, and commercial ports are the main sources of contaminants to the area. | YU Ruilian YUAN Xing ZHAO Yuanhui HU Gongren TU Xianglin | 2008 | Journal of Environmental Sciences2008，20，6： | 73 |
| 4 | Optical vortices 30 years on:OAM manipulation from topological charge to multiple singularities显示文摘Thirty years ago,Coullet et al.proposed that a special optical field exists in laser cavities bearing some analogy with the superfluid vortex.Since then,optical vortices have been widely studied,inspired by the hydrodynamics sharing similar mathematics.Akin to a fluid vortex with a central flow singularity,an optical vortex beam has a phase singularity with a certain topological charge,giving rise to a hollow intensity distribution.Such a beam with helical phase fronts and orbital angular momentum reveals a subtle connection between macroscopic physical optics and microscopic quantum optics.These amazing properties provide a new understanding of a wide range of optical and physical phenomena,including twisting photons,spin–orbital interactions,Bose-Einstein condensates,etc.,while the associated technologies for manipulating optical vortices have become increasingly tunable and flexible.Hitherto,owing to these salient properties and optical manipulation technologies,tunable vortex beams have engendered tremendous advanced applications such as optical tweezers,high-order quantum entanglement,and nonlinear optics.This article reviews the recent progress in tunable vortex technologies along with their advanced applications. | Yijie Shen Xuejiao Wang Zhenwei Xie Changjun Min Xing Fu Qiang Liu Mali Gong Xiaocong Yuan | 2019 | Light(Science & Applications)2019，8，1： | 77 |
| 5 | 2018 Chinese Pediatric Cardiology Society(CPCS) guideline for diagnosis and treatment of syncope in children and adolescents显示文摘Syncope belongs to the transient loss of consciousness(TLOC), characterized by a rapid onset, short duration, and spontaneous complete recovery. It is common in children and adolescents, accounting for 1% to 2% of emergency department visits.Recurrent syncope can seriously affect children's physical and mental health, learning ability and quality of life and sometimes cardiac syncope even poses a risk of sudden death. The present guideline for the diagnosis and treatment of syncope in children and adolescents was developed for guiding a better clinical management of pediatric syncope. Based on the globally recent development and the evidence-based data in China, 2018 Chinese Pediatric Cardiology Society(CPCS) guideline for diagnosis and treatment of syncope in children and adolescents was jointly prepared by the Pediatric Cardiology Society, Chinese Pediatric Society, Chinese Medical Association(CMA)/Committee on Pediatric Syncope, Pediatricians Branch, Chinese Medical Doctor Association(CMDA)/Committee on Pediatric Cardiology, Chinese College of Cardiovascular Physicians, Chinese Medical Doctor Association(CMDA)/Pediatric Cardiology Society, Beijing Pediatric Society, Beijing Medical Association(BMA). The present guideline includes the underlying diseases of syncope in children and adolescents, the diagnostic procedures, methodology and clinical significance of standing test and headup tilt test, the clinical diagnosis vasovagal syncope, postural orthostatic tachycardia syndrome, orthostatic hypotension and orthostatic hypertension, and the treatment of syncope as well as follow-up. | Cheng Wang Yaqi Li Ying Liao Hong Tian Min Huang Xiangyu Dong Lin Shi Jinghui Sun Hongfang Jin Junbao Du Jindou An Jie Chen Mingwu Chen Qi Chen Sun Chen Yonghong Chen Zhi Chen Adolphus Kai-tung Chau Junbao Du Zhongdong Du Junkai Duan Hongyu Duan Xiangyu Dong Lin Feng Lijun Fu Fangqi Gong Yonghao Gui Ling Han Zhenhui Han Bing He Zhixu He Xiufen Hu Yimin Hua Guoying Huang Min Huang Ping Huang Yujuan Huang Hongfang Jin Mei Jin Bo Li Fen Li Tao Li Xiaohui Li Xiaoyan Liu Yan Li Haitao Lv Tiewei Lv Zipu Li Luyi Ma Silin Pan Yusheng Pang Hua Peng Yuming Qin Jie Shen Lin Shi Kun Sun Jinghui Sun Hong Tian Jie Tian Cheng Wang Hong Wang Lei Wang Jinju Wang Wendi Wang Yuli Wang Rongzhou Wu Tianhe Xia Yanyan Xiao Chunhong Xie Yanlin Xing Zhenyu Xiong Baoyuan Xu Yi Xu Hui Yan Shiwei Yang Qijian Yi Xia Yu Xianyi Yu Yue Yuan Hongyan Zhang Huili Zhang Li Zhang Qingyou Zhang Xi Zhang Yanmin Zhang Zhiwei Zhang Cuifen Zhao Bin Zhou Hua Zhu | 2018 | Science Bulletin2018，63，23： | 54 |
| 6 | 8-bromo-7-methoxychrysin-induced apoptosis of hepatocellular carcinoma cells involves ROS and JNK显示文摘AIM:To investigate whether the apoptotic activities of 8-bromo-7-methoxychrysin(BrMC) involve reactive oxygen species(ROS) generation and c-Jun N-terminal kinase(JNK) activation in human hepatocellular carcinoma cells(HCC).METHODS:HepG2,Bel-7402 and L-02 cell lines were cultured in vitro and the apoptotic effects of BrMC were evaluated by flow cytometry(FCM) after propidium iodide(PI) staining,caspase-3 activity using enzymelinked immunosorbent assay(ELISA),and DNA agarose gel electrophoresis.ROS production was evaluated by FCM after dichlorodihydrofluorescein diacetate(DCHFDA) probe labeling.The phosphorylation level of JNK and c-Jun protein was analyzed by Western blotting.RESULTS:FCM after PI staining showed a dose-dependent increase in the percentage of the sub-G1 cell pop-ulation(P < 0.05),reaching 39.0% ± 2.8% of HepG2 cells after 48 h of treatment with BrMC at 10 μmol/L.The potency of BrMC to HepG2 and Bel-7402(32.1% ± 2.6%) cells was found to be more effective than the lead compound,chrysin(16.2% ± 1.6% for HepG2 cells and 11.0% ± 1.3% for Bel-7402 cell) at 40 μmol/L and similar to 5-flurouracil(33.0% ± 2.1% for HepG2 cells and 29.3% ± 2.3% for Bel-7402 cells) at 10 μmol/L.BrMC had little effect on human embryo liver L-02 cells,with the percentage of sub-G1 cell population 5.4% ± 1.8%.Treatment of HepG2 cells with BrMC for 48 h also increased the levels of active caspase-3,in a concentration-dependent manner.z-DEVD-fmk,a caspase-3specific inhibitor,prevented the activation of caspase-3.Treatment with BrMC at 10 μmol/L for 48 h resulted in the formation of a DNA ladder.Treatment of cells with BrMC(10 μmol/L) increased mean fluorescence intensity of DCHF-DA in HepG2 cells from 7.2 ± 1.12 at 0 h to 79.8 ± 3.9 at 3 h and 89.7 ± 4.7 at 6 h.BrMC did not affect ROS generation in L-02 cells.BrMC treatment failed to induce cell death and caspase-3 activation in HepG2 cells pretreated with N-acetylcysteine(10 mmol/L).In addition,in HepG2 cells treated with BrMC(2.5,5.0,10.0 μmol/L) for 12 h,JNK activation was observed.Peak JNK activation occurred at 12 h post-treatment and this activation persisted for up to 24 h.The expression of phosphorylated JNK and c-Jun protein after 12 h with BrMC-treated cells was inhibited by N-acetylcysteine and SP600125 pre-treatment,but GW9662 had no effect.SP600125 substantially reduced BrMC-induced cell death and caspase-3 activation of HepG2 cells.N-acetylcysteine and GW9662 also attenuated induction of cell death and caspase-3 activation in HepG2 cells treated with BrMC.CONCLUSION:BrMC induces apoptosis of HCC cells by ROS generation and sustained JNK activation. | Xiao-Hong Yang Jian-Guo Cao Hong-Lin Xiang Fei Liu Yuan Lv Hunan Province China Xing Zheng | 2010 | World Journal of Gastroenterology2010，16，27： | 38 |
| 7 | Tumor-secreted miR-214 induces regulatory T cells: a major link between immune evasion and tumor growth显示文摘 | Yuan Yin Xing Cai Xi Chen Hongwei Liang Yujing Zhang Jing Li Zuoyun Wang Xiulan Chen Wen Zhang Seiji Yokoyama Cheng Wang Liang Li Limin Li Dongxia Hou Lei Dong Tao Xu Takachika Hiroi Fuquan Yang Hongbin Ji Junfeng Zhang Ke Zen Chen-Yu Zhang | 2014 | Cell Research2014，24，10： | 33 |
| 8 | The state-of-the-art of the China Seismo-Electromagnetic Satellite mission显示文摘The China Seismo-Electromagnetic Satellite(CSES) mission was proposed in 2003 and approved in 2013 after ten years' scientific and engineering demonstrations. To meet the requirement of scientific objectives, the satellite is designed to be in a sunsynchronous orbit with an altitude of 507 km and descending node time of 14:00 LT. The CSES satellite carries 8 instruments,including search-coil magnetometer(SCM), electric field detector(EFD), high precision magnetometer(HPM), GNSS occultation receiver(GOR), plasma analyzer package(PAP), langmuir probe(LAP), high energetic particle package(HEPP) and detector(HEPD), and tri-band beacon(TBB), among which HEPD is provided by Italian Space Agency. The CSES satellite was launched successfully on February 2, 2018, and is planned to operate for 5 years. The CSES mission is the first satellite in China to measure geophysical fields, which will have a lot of application prospects in the study of seismology, geophysics, space sciences, and so on. | SHEN XuHui ZHANG XueMin YUAN ShiGeng WANG LanWei CAO JinBin HUANG JianPing ZHU Xing Hong PIERGIORGIO Picozzo DAI JianPing | 2018 | Science China(Technological Sciences)2018，61，5： | 33 |
| 9 | Nutritional and medicinal characteristics of Chinese giant salamander (Andrias davidianus) for applications in healthcare industry by artificial cultivation: A review显示文摘Andrias davidianus,i.e.Chinese giant salamander(CGS),is one of the largest and oldest amphibians existing in the world and is also one of the valuable biological resources of China.Wild CGS has been threatened with extinction in the past decades due to over capturing,deterioration of natural environment,the slow breeding and growth of the wild species in nature.However,in the past twenty years,with the breakthrough and progress of artificial breeding technology by artificial insemination,the number of artificially cultivated CGS has increased rapidly.Artificially cultivated CGS can either be released to the CGS living environment to increase the population in nature or legally applied in food and medicinal industry as a feedstock due to the unique nutritional and medicinal values of CGS as recorded historically.In this review,the nutritional components,bioactive components and medicinal activities of the artificially cultivated CGS will be summarized.The mucus,skin,meat and bone of CGS contain many different bioactive substances thereby having various medicinal activities including anti-aging,anti-fatigue,anti-tumor,therapy of burn and anti-infection and other physiological functions.This paper will further discuss the potential applications of the artificially cultivated CGS in healthcare industry and prospects of future technological development. | Dong He Wenming Zhu Wen Zenga Jun Lin Yang Ji Yi Wang Chong Zhang Yuan Lu Daoquan Zhao Nan Su Xin-Hui Xing | 2018 | Food Science and Human Wellness2018，7，1： | 28 |
| 10 | A new cytotoxic 2-(2-phenylethyl)chromone from Chinese eaglewood显示文摘新复合 8-chloro-5,6,7-trihydroxy-2-(3-hydroxy-4-methoxyphenethyl )-5,6,7,8-tetrahydro-4H-chromen-4-one (1 ) 从中国沉香被孤立[Aquilaria sinensis (愁相) Gilg ] 。它的结构被阐明根据光谱数据。与 14.6 渭 g /mL 的 IC50 价值由 MTT 方法在 vitro 对人的胃的癌症房间线(SGC-7901 ) 加重 1 显示出的 cytotoxicity。 | Jun Liu Jiao Wu You Xing Zhao Yuan Yuan Deng Wen Li Mei Hao Fu Dai | 2008 | Chinese Chemical Letters2008，19，8： | 25 |
| 11 | Autophagy in bone homeostasis and the onset of osteoporosis显示文摘Autophagy is an evolutionarily conserved intracellular process,in which domestic cellular components are selectively digested for the recycling of nutrients and energy.This process is indispensable for cell homeostasis maintenance and stress responses.Both genetic and functional studies have demonstrated that multiple proteins involved in autophagic activities are critical to the survival,differentiation,and functioning of bone cells,including osteoblasts,osteocytes,and osteoclasts.Dysregulation at the level of autophagic activity consequently disturbs the balance between bone formation and bone resorption and mediates the onset and progression of multiple bone diseases,including osteoporosis.This review aims to introduce the topic of autophagy,summarize the understanding of its relevance in bone physiology,and discuss its role in the onset of osteoporosis and therapeutic potential. | Xing Yin Chenchen Zhou Jingtao Li Renkai Liu Bing Shi Quan Yuan Shujuan Zou | 2019 | Bone Research2019，7，4： | 25 |
| 12 | Inducing apoptosis and upregulation of Bax and Fas ligand expression by allicin in hepatocellular carcinoma in Balb/c nude mice显示文摘 | ZHANG Zhi-mian ZHONG Ning GAO Hai-qing ZHANG Shang-zhong WEI Yuan XIN Hua MEI Xing HOU Huai-shui LIN Xi-yun SHI Qing | 2006 | Chinese Medical Journal2006，，5： | 24 |
| 13 | Curcumin inhibits cellular cholesterol accumulation by regulating SREBP-1/caveolin-1 signaling pathway in vascular smooth muscle cells显示文摘目的: 在 anti-atherosclerosis.Methods 上调查保护的效果和杂种狗枯茗的可能的机制: 从 apoE 大美人拿的动脉粥样硬化患者损害的词法变化(apoE^(-/-))mice 被染色的 hematoxylin 曙红决定。细胞内部的类脂化合物微滴和类脂化合物层次是由油红O染色的 assayed , caveolin-1 的 HPLC.The 蛋白质表示被西方的 blotting.Translocation 和甾醇反应元素绑定 protein-1 ( SREBP-1 )的表示确定被免疫荧光 analysis.Results 间接地检测: 20 mg ·k g~的管理( -1)·d^(-1)curcumin 到apoE^(为 4 个月的 -/-)mice 导致了动脉粥样硬化患者损害的50%减小并且作为与模型组相比在 caveolin-1 表示水平产出5褶层增加。有 50 mg ·L ~的老鼠脉管的平滑肌房间( VSMC ) pretreated (为 48 h 的 -1)ox-lipid 密度脂蛋白( ox-LDL )增加了细胞的类脂化合物内容,并且刺激了 SREBP-1 易位,但是减少 装载level.Lipid 的房间在各种各样的集中暴露了到杂种狗枯茗的 caveolin-1 表示( 12.5,25 ,并且 50 亩摩尔 ·L ^( -1))for 不同持续时间( 0,6,12,24 ,和 48 h )显著地减少了细胞的类脂化合物微滴的数字和区域,全部的胆固醇,胆固醇酉旨,并且伴随 caveolin-1 表示的举起的免费胆固醇铺平(近似3褶层);到原子核的从细胞质的 SREBP-1 的易位与暴露于 N-acetyl-Leu-Leu-norleucinal 的 装载models.Lipid 的 VSMC 相比被禁止,一个 SREBP-1 朊酶禁止者, SREBP-1 的显示出的增加的原子易位,减少的 caveolin-1 表示水平,并且在调整细胞的类脂化合物 levels.Conclusion 上面:姜黄色素通过经由 SREBP-1 的原子易位的抑制增加 caveolin-1 表示在有教养的 VSMC 禁止 ox-LDL-induced 胆固醇累积。 | Hao-yu YUAN Shuang-yu KUAN Xing ZHENG Hong-yan LING Yun-Bo YANG Peng-Ke YAN Kai LI Duan-Fang LIAO | 2008 | Acta Pharmacologica Sinica2008，29，5： | 21 |
| 14 | Antihepatoma effect of alpha-fetoprotein antisense phosphorothioate oligodeoxyribonucleotides in vitro and in mice显示文摘AIM To evaluate antihepatoma effect ofantisense phosphorothioate oligodeo-xyribonucleotides (S-ODNs) targeted to alpha-fetoprotein (AFP) genes in vitro and in nudemice.METHODS AFP gene expression was examinedby immunocytochemical method or enzyme-linked immunosorbent assay. Effect of S-ODNson SMMC-7721 human hepatoma cell growth invitro was determined using microculturetetrazolium assay. In vivo antitumor activitiesof S-ODNs were monitored by measuring tumorweight differences in treated and control micebearing SMMC-7721 xenografts. Induction of cellapoptosis was evaluated by fluorescence-activated cell sorter (FACS) analysis.RESULTS Antisense S-ODN treatment led toreduced AFP gene expression. Specificantisense S-ODNs, but not control S-ODNs,inhibited the growth of heaptoma cells in vitro.In vivo. only antisense S-ODNs exhibitedobvious antitumor activities. FACS analysisrevealed that the growth inhibition by antisenseS. ODNs was associated with their cell apoptosisinduction.CONCLUSION Antisense S-ODNs targeted toAFP genes inhibit the growth of human hepatomacells and solid hepatoma, which is related totheir cell apoptosis induction. | Xing Wang Wang~1 Jin Hui Yuan~1 Ru Gang Zhang~1 Li Xia Guo~1 Yong Xie~2 Hong Xie~1 ~1Department of Biotherapy,Shanghai Institute of Cell Biology,Chinese Academy of Sciences,Shanghai 200031,China ~2Department of Biology,Hong Kong University of Science and Technology,ChinaDr.Xing Wang Wang earned Ph.D.from Shanghai Institute of Materia Medical,Chinese Academy of Sciences in 1997.Now a professor at Shanghai Institute of Cell Biology,Chinese Academy of Sciences. | 2001 | World Journal of Gastroenterology2001，7，3： | 21 |
| 15 | Long-term results of endovascular therapy for proximal subclavian arterial obstructive lesions显示文摘背景 Endovascular 治疗是为锁骨下的动脉的局部性的吸藏的一种处理选择。在与 59 个病人一起的长期的经验是在1998年6月和2008年9月之间的 presented.Methods 的这份报告,我们使用了 endovascular 治疗在 59 个病人对待 61 锁骨下的动脉的妨碍的损害( 46 男性和 13 女性,有一个平均数的 34-82 岁变老( 61.9 眠瑩?佃?????????????? | WANG Ke-qin WANG Zhong-gao YANG Bao-zhong YUAN Chao ZHANG Wang-de YUAN Biao XING Tong SONG Sheng-han LI Tan LIAO Chuan-jun ZHANG Yang | 2010 | Chinese Medical Journal2010，，1： | 21 |
| 16 | Clinical and radiographic results of cervical artificial disc arthroplasty: over three years follow-up cohort study显示文摘颈的圆盘关节造形术的背景理论上的优点在邻近片断的退化的颈的脊骨,和减小包括正常运动和 biomechanics 的保藏。在短术语的颈的圆盘关节造形术的临床、 X 光线照相术的效果被查明了。这研究的目的是在这未来的队研究研究中间的 results.Methods 的数据,从 2003 年 12 月经历了颈的磁盘关节造形术到 2006 年 1 月的 50 个病人被注册。有 39 个病人,收到了 1 水平圆盘关节造形术,并且 11 个病人收到了 2 水平圆盘关节造形术,与 50.9 年(从 29 ~ 73 的范围) 的平均年龄。中部的后续是 41.85months (从 36.00-55.63 月的范围) 。病人们关于他们日本整形外科的协会(JOA ) 分数的中部的价值是的症状,神经症状,和 X 光线照相术的 results.Results 有希望地被跟随 14.0 在外科前,并且 16.5 在最近的后续(P 0.01 ) 。JOA 分数的恢复率的中部的价值是 92.2% 。在索引的水平的运动(ROM ) 的外科手术前的范围是(10.40 吗?? | TIAN Wei HAN Xiao LIU Bo LI Qin HU Lin LI Zhi-yu YUAN Qiang HE Da XING Yong-gang | 2010 | Chinese Medical Journal2010，，21： | 19 |
| 17 | Laparoscopic resection of lower rectal cancer with telescopic anastomosis without abdominal incisions显示文摘AIM:To assess laparoscopic radical resection of lower rectal cancer with telescopic anastomosis through transanal resection without abdominal incisions.METHODS:From March 2010 to June 2014, 30 patients(14 men and 16 women, aged 36-78 years, mean age 59.8 years) underwent laparoscopic radical resection of lower rectal cancer with telescopic anastomosis through anus-preserving transanal resection.The tumors were 5-7 cm away from the anal margin in 24 cases, and 4 cm in six cases.In preoperative assessment, there were 21 cases of T1N0M0 and nine of T2N0M0.Through the middle approach, the sigmoid mesentery was freed at the root with an ultrasonic scalpel and the roots of the inferior mesenteric artery and vein were dissected, clamped and cut.Following the total mesorectal excision principle, the rectum was separated until the anorectal ring reached 3-5 cm from the distal end of the tumor.For perineal surgery, a ring incision was made 2 cm above the dentate line, and sharp dissection was performed submucosally towards the superior direction, until the plane of the levator ani muscle, to transect the rectum.The rectum and distal sigmoid colon were removed together from the anus, followed by a telescopic anastomosis between the full thickness of the proximal colon and the mucosa and submucosal tissue of the rectum.RESULTS:For the present cohort of 30 cases,the mean operative time was 178 min,with an average of 13 positive lymph nodes detected.One case of postoperative anastomotic leak was observed,requiring temporary colostomy,which was closed and recovered3 mo later.The postoperative pathology showed T1-T2N0M0 in 19 cases and T2N1M0 in 11 cases.Twelve months after surgery,94.4%patients achieved anal function Kirwan grade 1,indicating that their analfunction returned to normal.The patients were followed up for 1-36 mo,with an average of 23 mo.There was no local recurrence,and 17 patients survived for>3years(with a survival rate of 100%).CONCLUSION:Laparoscopic radical resection of lower rectal cancer with telescopic anastomosis through transanal resection without abdominal incisions is safe and feasible. | Shi-Yong Li Gang Chen Jun-Feng Du Guang Chen Xiao-Jun Wei Wei Cui Fu-Yi Zuo Bo Yu Xing Dong Xi-Qing Ji Qiang Yuan | 2015 | World Journal of Gastroenterology2015，21，16： | 19 |
| 18 | Growth-inhibiting effects of taxol on human liver cancer in vitro and in nude mice显示文摘AIM To investigate the effects of taxol onSMMC-7721 human hepatoma and itsmechanisms.METHODS In vitro cell growth was assessedby trypan blue exclusion method.Experimentalhepatoma model was established by seedingSMMC-7721 cells subcutaneously into Balb/c(nu/nu)nude mice.In vivo tumor growth wasdetermined by measurement of tumor diameterwith Vernier calipers.The syntheses of DNA,RNA and protein were analyzed by incorporationof ~3H-thymidine,~3H-uridine and ~3H-leucinerespectively.Using light and electronmicroscopes to observe the morphologicalchanges of cells including mitosis andapoptosis.RESULTS Taxol was effective against SMMC-7721 human hepatoma cell growth in the rangesof 2.5 nmol/L-10 nmol/L with mitotic arrestand apoptosis in vitro.DNA,RNA and proteinsyntheses in cells were also obviouslysuppressed by in vitro treatment of taxol for72 h.Taxol at 2.5 nmol/L reduced ~3H-thymidineuptake to about 34% of the control value(P<0.05).Increasing the dose of taxol to20 nmol/L resulted in a greater decrease in ~3H-thymidine incorporation to 60% of the controlvalue(P<0.01).At a concentration of 20 nmol/L,the ~3H-uridine and ~3H-leucine uptakeswere reduced to 52%(P<0.05)and 63%(P<0.01),respectively.In vivo,taxolsignificantly inhibited SMMC-7721 tumor growthat 10 mg/kg,i.p.,once daily for 10 d.A morethan 90% decrease in tumor volume wasobserved by day 11(P<0.01)similarly withmitotic arrest and cell apoptosis.CONCLUSION Taxol has a marked anticanceractivity in SMMC-7721 human hepatoma both invitro and in nude mice.Its mechanisms might beassociated with mitotic arrest,subsequently,apoptosis of the hepatoma cells.No obvioustoxicity was observed with in vivoadministration of taxoi. | Jin Hui Yuan Ru Ping Zhang Ru Gang Zhang Li Xia Guo Xing Wang Wang Dan Luo Yong Xie Hong Xie | 2000 | World Journal of Gastroenterology2000，6，2： | 18 |
| 19 | Porphyrin-like Fe-N4 sites with sulfur adjustment on hierarchical porous carbon for different rate-determining steps in oxygen reduction reaction显示文摘We developed a strategy based on coordination polymer to synthesize singleatom site Fe/N and S-codoped hierarchical porous carbon (Fe1/N,S-PC).The as-obtained Fe1/N,S-PC exhibited superior oxygen reduction reaction (ORR) performance with a half-wave potential (Ev2,0.904 V vs.RHE) that was better than that of commercial Pt/C (E1/2,0.86 V vs.RHE),single-atom site Fe/N-doped hierarchical porous carbon (Fe1/N-PC) without S-doped (E1/2,0.85 V vs.RHE),and many other nonprecious metal catalysts in alkaline medium.Moreover,the Fe1/N,S-PC revealed high methanol tolerance and firm stability.The excellent electrocatalytic activity of Fe1/N,S-PC is attributed to the synergistic effects from the atomically dispersed porphyrin-like Fe-N4 active sites,the heteroatom codoping (N and S),and the hierarchical porous structure in the carbon materials.The calculation based on density functional theory further indicates that the catalytic performance of Fe1/N,S-PC is better than that of Fe1/N-PC owing to the sulfur doping that yielded different rate-determining steps. | Konglin Wu Xin Chen Shoujie Liu Yuan Pan Weng-Chon Cheong Wei Zhu Xing Cao Rongan Shen Wenxing Chen Jun LUO Wensheng Yan Lirong Zheng Zheng Chen Dingsheng Wang Qing Peng Chen Chen Yadong Li | 2018 | Nano Research2018，11，12： | 18 |
| 20 | Chemical constituents,biological functions and pharmacological effects for comprehensive utilization of Eucommia ulmoides Oliver显示文摘Eucommia ulmoides Oliver is a native plant and valuable tonic Chinese medicine in China with a long history,great economic value and comprehensive development potential.Traditionally,the comprehensive utilization rate of E.ulmoides Oliv.is still very low,only bark has been used as medicine and other parts of Eucommia ulmoides Oliv.cannot be fully utilized,even the leaves have been well utilized in food products in Japan in the past decades.In order to improve the comprehensive utilization efficiency of E.ulmoides Oliv.,in this review,we summarized the varieties and contents of main active compounds,biological functions and pharmacological effects in different parts of E.ulmoides Oliv.The findings suggest that other parts of E.ulmoides Oliv.could replace the bark of E.ulmoides Oliv.to some extent besides of their respective applications.The unique and extensive physiological functions between different parts of E.ulmoides Oliv.indicate that the comprehensive utilization of E.ulmoides Oliv.has a wide space to develop,which is also an effective way to protect E.ulmoides Oliv.resources and improve its the utilization rate. | Yi-Fan Xing Dong He Yi Wang Wen Zeng Chong Zhang Yuan Lu Nan Su Yan-Hua Kong Xin-Hui Xing | 2019 | Food Science and Human Wellness2019，8，2： | 18 |
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