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| 1 | A complete sequence and comparative analysis of a SARS-associated virus(Isolate BJ01)显示文摘The genome sequence of the Severe Acute Respiratory Syndrome (SARS)-associated virus provides essential information for the identification of pathogen(s), exploration of etiology and evolution, interpretation of transmission and pathogenesis, development of diagnostics, prevention by future vaccination, and treatment by developing new drugs. We report the complete genome sequence and comparative analysis of an isolate (BJ01) of the coronavirus that has been recognized as a pathogen for SARS. The genome is 29725 nt in size and has 11 ORFs (Open Reading Frames). It is composed of a stable region encoding an RNA-dependent RNA polymerase (composed of 2 ORFs) and a variable region representing 4 CDSs (coding sequences) for viral structural genes (the S, E, M, N proteins) and 5 PUPs (putative uncharacterized proteins). Its gene order is identical to that of other known coronaviruses. The sequence alignment with all known RNA viruses places this virus as a member in the family of Coronaviridae. Thirty putative substitutions have been identified by comparative analysis of the 5 SARS- associated virus genome sequences in GenBank. Fifteen of them lead to possible amino acid changes (non-synonymous mutations) in the proteins. Three amino acid changes, with predicted alteration of physical and chemical features, have been detected in the S protein that is postulated to beinvolved in the immunoreactions between the virus and its host. Two amino acid changes have been detected in the Mprotein, which could be related to viral envelope formation. Phylogenetic analysis suggests the possibility of non-human origin of the SARS-associated viruses but provides noevidence that they are man-made. Further efforts should focus on identifying the etiology of the SARS-associated virus and ruling out conclusively the existence of otherpossible SARS-related pathogen(s). | QIN E'de ZHU Qingyu YU Man FAN Baochang CHANG Guohui SI Bingyin YANG Bao PENG Wenming JIANG Tao LIU Bohua DENG Yongqiang LIU Hong ZHANG Yu WANG Cui LI Yuquan GAN Yonghua LI Xiaoyu L Fushuang TAN Gang CAO Wuchun, YANG Ruifu Institute of Microbiology and Epidemiology, Chinese Academy of Military Medical Sciences, Beijing 100071, China WANG Jian, LI Wei, XU Zuyuan, LI Yan, WU Qingfa, LIN Wei, CHEN Weijun, TANG Lin, DENG Yajun, HAN Yujun, LI Changfeng, LEI Meng, LI Guoqing, LI Wenjie, L Hong, SHI Jianping, TONG Zongzhong, ZHANG Feng, LI Songgang, LIU Bin, LIU Siqi, DONG Wei, WANG Jun, Gane K-S Wong, YU Jun & YANG Huanming* Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300 National Center for Genome Information, Beijing 101300, China | 2003 | Chinese Science Bulletin2003，48，10： | 121 |
| 2 | Atomically dispersed Au_1 catalyst towards efficient electrochemical synthesis of ammonia显示文摘Tremendous efforts have been devoted to explore energy-efficient strategies of ammonia synthesis to replace Haber–Bosch process which accounts for 1.4% of the annual energy consumption. In this study,atomically dispersed Au_1 catalyst is synthesized and applied in electrochemical synthesis of ammonia under ambient conditions. A high NH_4^+ Faradaic efficiency of 11.1% achieved by our Au_1 catalyst surpasses most of reported catalysts under comparable conditions. Benefiting from efficient atom utilization, an NH_4^+ yield rate of 1,305 lg h^(-1) mg_(Au)^(-1) has been reached, which is roughly 22.5 times as high as that by supported Au nanoparticles. We also demonstrate that by employing our Au_1 catalyst, NH4+can be electrochemically produced directly from N_2 and H_2 with an energy utilization rate of 4.02 mmol kJ^(-1). Our study provides a possibility of replacing the Haber–Bosch process with environmentally benign and energy-efficient electrochemical strategies. | Xiaoqian Wang Wenyu Wang Man Qiao Geng Wu Wenxing Chen Tongwei Yuan Qian Xu Min Chen Yan Zhang Xin Wang Jing Wang Jingjie Ge Xun Hong Yafei Li Yuen Wu Yadong Li | 2018 | Science Bulletin2018，63，19： | 22 |
| 3 | A High-Throughput Screening System for Arabidopsis Transcription Factors and Its Application to Med25-Dependent Transcriptional Regulation显示文摘抄写因素(TF ) 的活动与特定的 DNA 序列和另外的规章的蛋白质要求相互作用。在 Arabidopsis 检测如此的相互作用,我们与 1589 Arabidopsis TF 的一个网关兼容机 Gal4-ADTF 图书馆开发了一个高产量的屏蔽系统,它能被基于配偶的 yeast-one-hybrid (Y1H ) 和 yeast-two-hybrid (Y2H ) 容易屏蔽方法。系统的效率被检验二个描绘得好的 TFDNA 和 TFprotein 相互作用验证:由由 Y2H 的 Y1H 和 NPR1TGAs 相互作用的 CHECCA1 倡导者相互作用。我们使用了这个系统识别与一个调停人子单元交往的八 TF, Med25,在 JA 发信号的一个关键管理者。我们识别了在 PDF1.2 的倡导者与 GCC 盒子 cis 元素交往了的五 TF, Med25 的下游的基因。我们发现都从 AP2-EREBP 家庭,三这些 TF 与 Med25 和 PDF1.2 的 GCC 盒子两个都直接交往,建议 Med25 调整通过这三 TF 的 PDF1.2 表示。这些结果证明屏蔽系统的这高产量的 Y1H/Y2H 是为在 Arabidopsis 学习 transcriptional 规定网络的一个有效工具。这个系统将为另外的 Arabidopsis 研究人员是可得到的,并且因此,它为 Arabidopsis 社区提供一个重要资源。 | Bin Oua Kang-Quan Yin Sai-Nan Liu Yan Yang Tren Gu Jennifer Man Wing Hui Li Zhang Jin Miao Youichi Kondou Minami Matsui Hong-Ya Gu Li-Jia Qu | 2011 | Molecular Plant2011，4，3： | 19 |
| 4 | Effects of advanced glycation end products on renal fibrosis and oxidative stress in cultured NRK-49F ceils显示文摘背景先进 glycation 结束产品(年龄) 在糖尿病的 nephropathy 的发展起一个关键作用。反应的氧种类(ROS ) 可以在年龄起一个关键作用导致的生长因素表示。在这研究,转变生长因素 1 上的年龄( TGF-1 )的效果,结缔组织生长因素( CTGF )和在有教养的 NRK-49F 细胞的 mRNA 表示和氧化应力是的 fibronectin ( Fn ), examined.Methods NRK-49F 细胞与包含年龄的不同剂量的媒介被孵化( 50 , 100 或 200 g/ml )为 24 个小时,或与年龄在不同时间( 0 , 12 , 24 或 48 个小时)的 100 g/ml 。在包含 25 mmol/L 葡萄糖的没有浆液的媒介或媒介的房间是控制。房间与 25 mmol/L 葡萄糖和 100 g/ml 年龄被对待 24 个小时决定在年龄和葡萄糖之间的效果。我们与 N-acetylcysteine (10 mmol/L ) 由 pretreating 房间澄清了抗氧化剂的角色,为 24 个小时并且与 100 g/ml 的白果树 biloba 摘录(50 或 100 mg/L ) 变老为推进 24 个小时。Alamarblue 染料试金被用来分析房间生长;细胞内部的 ROS 产生被流动 cytometry 测量;由荧光 spectrophotometry 的细胞内部的谷胱甘肽;到 semiquantitative RT-PCR.Results 年龄的 TGF-1, CTGF 和 Fn mRNA 的表情显著地增加了 TGF-1, CTGF, Fn mRNA 和细胞内部的 ROS 产生的表情,并且减少在在剂量依赖者和时间依赖者礼貌的 NRK-49F 房间的 glutathion 水平。高葡萄糖和年龄一起显著地增加了 TGF-1, CTGF 和 Fn mRNA 的表示,与年龄和高葡萄糖相比独立。有 N-acetylcysteine 或白果树 biloba 摘录的 Preincubation 增加了 GSH mRNA overexpression.Conclusions 变老的水平,压制的导致年龄的氧化压力和 TGF-1, CTGF 和 Fn 显著地装 TGF-1 的增加表示, CTGF,在通过氧化应力的改进的 NRK-49F 房间的 Fn mRNA。年龄的累积可以在糖尿病的 nephropathy 在 tubulointerstitial 纤维变性的致病起一个枢轴的作用。年龄的抑制导致了 TGF-1, CTGF 和 Fn mRNA 在通过氧化应力的抑制的肾的成纤维细胞的 overexpression 可以是在糖尿病的 nephropathy 位于白果树 biloba 摘录的效果下面的机制。另外,抗氧化剂治疗可以帮助阻止年龄累积和它的导致的损坏。 | YAN Hai-dong LI Xue-zhu XIE Jun-mei LI Man | 2007 | Chinese Medical Journal2007，，9： | 17 |
| 5 | Four IVa bHLH Transcription Factors Are Novel Interactors of FIT and Mediate JA Inhibition of Iron Uptake in Arabidopsis显示文摘工厂发展了复杂基因网络调整铁(Fe ) 为他们的幸存的动态平衡。包括 jasmonic 酸(JA ) 的工厂荷尔蒙的几个类被显示了涉及在工厂调整铁举起或缺乏应答的基因的表达式。然而, JA 由调整铁举起的分子的机制仍然保持不清楚。在这研究,我们发现 JA 否定地由 downregulating 调制铁举起表示合适(bHLH29 ) , bHLH38, bHLH39, bHLH100,和 bHLH101 并且支持合适的蛋白质的降级,在 Arabidopsis 的铁举起的一个关键管理者。我们进一步证明亚群 IVa bHLH 蛋白质, bHLH18, bHLH19, bHLH20,和 bHLH25,是新奇 interactors 适合,它支持导致 JA 的合适的蛋白质降级。这四 IVa bHLHs 冗余地工作反对 Ib bHLHs 的活动(例如 bHLH38 ) 在在铁下面调整合适的蛋白质稳定性缺乏。四 IVa bHLH 基因首先在根被表示,并且由 JA 处理是可诱导的。而且,我们发现了那 MYC2 和 JAR1 ,表明小径的 JA 的二个批评部件,在调停起关键作用表示的 JA 抑制合适并且 Ib bHLH 基因他们差别调制 bHLH18 的表示, bHLH19 , bHLH20 ,并且调整的 bHLH25 在铁缺乏下面适合累积。一起拿,这些结果显示由 transcriptionally 调整 bHLH 基因的不同集合的 ?表示,发信号的 JA 支持合适的降级,导致铁举起的减少的表示基因, IRT1 和 FRO2,和到铁的增加的敏感缺乏。我们的数据 ? 建议当面有铁缺乏反应的 multilayered 抑制在 Arabidopsis 的 JA。 | Yan Cui Chun-Lin Chen Man Cui Wen-Juan Zhou Hui-Lan Wu Hong-Qing Ling | 2018 | Molecular Plant2018，11，9： | 16 |
| 6 | Multicenter Evaluation of the Molecular Line Probe Assay for Multidrug Resistant Mycobacterium Tuberculosis Detection in China显示文摘In order to evaluate the performance of a molecular Hain line probe assay(Hain LPA)for rapid detection of rifampicin and isoniazid resistance of Mycobacterium tuberculosis in China。 | LI Qiang DONG Hai Yan PANG Yu XIA Hui OU Xi Chao ZHANG Zhi Ying LI Jun Chen ZHANG Jian Kang HUAN Shi Tong CHIN Daniel P KAM Kai Man ZHAO Yan Lin | 2015 | Biomedical and Environmental Sciences2015，28，6： | 13 |
| 7 | Dicer1/miR-29/HMGCR axis contributes to hepatic free cholesterol accumulation in mouse non- alcoholic steatohepatitis显示文摘Dicer1 是为 microRNA (miRNA ) 的酶必需品成熟。miRNAs 的损失源于 Dicer1 缺乏极大地贡献许多疾病的前进,包括在在非酒精的 steatohepatitis (NASH ) 的发展是批评的免费胆固醇(FC ) 的肝的累积的类脂化合物 dysregulation,而是它的角色遗体逃犯。在这研究,我们使用了肝特定的 Dicer1 大美人老鼠识别涉及肝的 FC 累积的 miRNAs。在一个广泛地使用的饮食的 NASH 模型,老鼠被喂 methionine-choline-deficient (MCD ) 节食 3 个星期,它在肝象 Dicer1 mRNA 层次的减少一样导致了肝的 FC 层次的重要增加。肝特定的 Dicer1 大美人在 5-6 星期导致了肝的 FC 累积,由增加的 mRNA 和 3-hydroxy-3-methylglutaryl 辅酶的蛋白质层次伴随了 reductase (HMGCR ) ,在肝的胆固醇合成的限制率的酶。十一预言 miRNAs 被屏蔽,表明 miR-29a/b/c 显著地压制了由指向 HMGCR mRNA 3-UTR 的 HMGCR 表示。在 SMMC-7721 房间的 miR-29a 的 Overexpression,脂肪变性肝的房间模型,显著地减少的 HMGCR 表示和 FC 铺平。而且, miR-29a 的表达式层次相反地与在 MCD 食谱鼠标的表达式层次在 vitro 在 vivo 并且在肝的房间建模的 2 脂肪变性(SMMC-7721 和 HL-7702 房间) 建模的 HMGCR 被相关。我们的结果证明那 Dicer1/miR-29/HMGCR 轴在老鼠 NASH 贡献肝的免费胆固醇累积,并且 miR-29 可以用作肝的胆固醇动态平衡的一个重要管理者。因此, miR-29a 能为非酒精的脂肪肝疾病的处理以及为与 FC 累积联系的另外的肝疾病作为一个潜在的治疗学的目标被利用。 | Ming-xia LIU Man GAO Chun-zhu LI Cun-zhi YU Hong YAN Chun PENG Yu LI Cheng-gang LI Ze-long MA Yang ZHAO Meng-fan PU Ling-ling MIAO Xin-ming QI Jin RENI | 2017 | Acta Pharmacologica Sinica2017，38，5： | 12 |
| 8 | Analysis of Allergens in 5473 Patients with Allergic Diseases in Harbin, China显示文摘Objective To analyze the allergic status to common inhalant allergens and food allergens in clinical patients in Harbin in northeastern China and provide evidence to develop the prevention strategy of allergic disease. Methods The data were collected from 5473 patients with clinical suspected allergic diseases seeking medical care in the Second Affiliated Hospital of Harbin Medical University. Among these patients, 2530(46.2%) were males aged 0-86 years, the youngest was only 1 month old and 2579(47.1%) were young children and teenagers. The serum specific Immunoglobulin E(sIgE) to 14 kinds of common allergens and serum total IgE were detected by using AllergyScreen test(Mediwiss Analytic GmbH, Moers, Germany). Results In 5473 subjects the positive rate of sIgE was 33.1%(n=1813). Cow milk(6.9%) and wheat(3.1%) were the most common food allergens, followed by house dust mite mix(12.5%) and mould mix(9.4%) and the age and gender specific differences in the positive rate were significant. For the children aged <7 years the positive rates to cow milk, beef-mutton, and egg white/egg yolk were high, but the positive rates to house dust mite mix, ragweed estragon, and mould mix were low(P<0.05). For the adults the positive rates to aeroallergens were high while the rates to food allergens were low. Conclusion The results from this study showed that the food allergens in Harbin had geographic characteristics, which support the viewpoint that the environment factors play an important role in the incidence of allergic diseases. Also, the detection of sIgE and total IgE are essential to identify relevant allergens for the purpose of early diagnosis, management and prevention of allergic disease. | CHANG Man Li SHAO Bing LIU Yan Hong LI Lu Lu PEI Li Chun WANG Bin You | 2013 | Biomedical and Environmental Sciences2013，26，11： | 11 |
| 9 | Characterizing the optimal operation of photocatalytic degradation of BDE-209 by nano-sized TiO_2显示文摘Brominated flame retardants have been widely used in industry.There is a rapid growing public concern for their availabilities in the environment.Advanced oxidation process(AOP) is a promising and efficient technology which may be used to remove emerging chemicals such as brominated flame retardants.This study aims at investigating optimal operational conditions for the removal of BDE-209 using nano-scaled titanium(IV) oxide.The residual PBDE congeners after photocatalytical degradation of BDE-209 by TiO 2 were analysed by gas chromatography-mass spectrometry(GC-MS).It was found that the degradability of BDE-209 by TiO 2 was attributed to its photocatalytic activity but not the small size of the particles.The half-life of removing BDE-209 by TiO 2 was 3.05 days under visible light.Tetra-and penta-BDEs were the major degraded products of BDE-209.Optimum conditions for photocatalytical degradation of BDE-209 was found to be at pH 12(93% ± 1%),5,10,20 mg/L(93.0% ± 1.70%,91.6% ± 3.21%,91.9% ± 0.952%,respectively),respectively of humic acid and in the form of anatase/rutile TiO 2(82% ± 3%).Hence,the efficiency of removing BDE-209 can be maximized while being cost effective at the said operating conditions. | Ka Lai Chow Yu Bon Man Jin Shu Zheng Yan Liang Nora Fung Yee Tam Ming Hung Wong | 2012 | Journal of Environmental Sciences2012，24，9： | 8 |
| 10 | A Crucial Role of GA-Regulated Flavonol Biosynthesis in Root Growth of Arabidopsis显示文摘Flavonols have been demonstrated to play many important roles in plant growth, development, and communication with other organisms. Flavonol biosynthesis is spatiotemporally regulated by the subgroup 7 R2R3-MYB (SG7 MYB) transcription factors including MYB11/MYB12/MYB111. However, whether SG7- MYB activity is subject to post-translational regulation remains unclear. Here, we show that gibberellic acid (GA) inhibits flavonol biosynthesis via DELLA proteins in Arabidopsis. Protein-protein interaction analyses revealed that DELLAs (RGA and GAI) interacted with SG7 MYBs (MYB12 and MYB111) both in vitro and in vivo, leading to enhanced affinity of MYB binding to the promoter regions of key genes for flavonol biosynthesis and thus increasing their transcriptional levels. We observed that the level of auxin in the root tip was negatively correlated with root flavonol content. Furthermore, genetic assays showed that loss-of-function mutations in MYB 12, which is predominantly expressed in roots, partially rescued the short-root phenotype of the GA-deficient mutant ga1-3 by increasing root meristem size and mature cell size. Consistent with these observations, exogenous application of the flavonol quercetin restored the root meristem size of myb12 ga1-3 to that of ga1-3. Taken together, our data elucidate a molecular mechanism by which GA promotes root growth by directly reducing flavonol biosynthesis. | Huijuan Tan Cong Man Ye Xie Jijun Yan Jinfang Chu Jirong Huang | 2019 | Molecular Plant2019，12，4： | 8 |
| 11 | Allogeneic Vγ9Vδ2 T-cell immunotherapy exhibits promising clinical safety and prolongs the survival of patients with late-stage lung or liver cancer显示文摘Vγ9Vδ2 T cells are promising candidates for cellular tumor immunotherapy.Due to their HLA-independent mode of action,allogeneic Vγ9Vδ2 T cells can be considered for clinical application.To apply allogeneic Vγ9Vδ2 T cells in adoptive immunotherapy,the methodology used to obtain adequate cell numbers with optimal effector function in vitro needs to be optimized,and clinical safety and efficacy also need to be proven.Therefore,we developed a novel formula to improve the expansion of peripheralγδT cells from healthy donors.Then,we used a humanized mouse model to validate the therapeutic efficacy of expandedγδT cells in vivo;furthermore,the expandedγδT cells were adoptively transferred into late-stage liver and lung cancer patients.We found that the expanded cells possessed significantly improved immune effector functions,including proliferation,differentiation,and cancer cell killing,both in vitro and in the humanized mouse model.Furthermore,a phase I clinical trial in 132 late-stage cancer patients with a total of 414 cell infusions unequivocally validated the clinical safety of allogeneic Vγ9Vδ2 T cells.Among these 132 patients,8 liver cancer patients and 10 lung cancer patients who received≥5 cell infusions showed greatly prolonged survival,which preliminarily verified the efficacy of allogeneic Vγ9Vδ2 T-cell therapy.Our clinical studies underscore the safety and efficacy of allogeneic Vγ9Vδ2 T-cell immunotherapy,which will inspire further clinical investigations and eventually benefit cancer patients. | Yan Xu Zheng Xiang Mohammed Alnaggar Léonce Kouakanou Jiawei Li Junyi He Jiashuang Yang Yi Hu Yan Chen Li Lin Jianlei Hao Jingxia Li Jibing Chen Man Li Qingling Wu Christian Peters Qinghua Zhou Jianshuang Li Yingqing Liang Xiaohua Wang Baohui Han Meili Ma Dieter Kabelitz Kecheng Xu Wenwei Tu Yangzhe Wu Zhinan Yin | 2021 | Cellular & Molecular Immunology2021，18，2： | 8 |
| 12 | Long-term postoperative outcomes of bilateral lateral rectus recession vs unilateral recession-resection for intermittent exotropia显示文摘AIM: To discuss the long-term postoperative results of bilateral lateral rectus recession(BLR) and unilateral lateral rectus recession-medial rectus resection(RR) in therapy of intermittent exotropia.METHODS: We retrospectively analyzed 213 cases of intermittent exotropia who underwent surgery between2008 and 2010. The patients were grouped into BLR group and RR group. Motor outcomes were divided into three groups on the basis of the angle of deviation after surgery: overcorrection(esotropia/phoria >5△), orthophoria(esotropia/phoria ≤5△to exotropia/phoria ≤10△), and undercorrection/recurrence(exotropia/phoria >10△).Titmus test was used to evaluate stereoacuity, the stereoacuity <800s of arc meaned the patients had stereopsis. Surgical outcome including motor criteria and sensory status were compared at postoperative 6, 12,24 mo and at 36 mo examination between groups. RESULTS: At 12, 24 mo after surgery, the motor outcomes had no difference(P >0.05) between groups.However, the motor outcomes at 6, 36 mo were signally different in each group, indicating the success rate in RR group at 6mo was higher than that in BLR group(83.02%vs 82.24%, P <0.05) but the result was contrary at the 3y examination(60.75% vs 43.40%, P <0.05). No statistical significance were found in the sensory outcomes between the groups at mean of 3.7y follow-up.CONCLUSION: The motor outcomes in RR group were better than in BLR group at 6mo after surgery, while the3 y outcomes were better in BLR group. This may be due to the recurrence rate of the BLR was lower than the RR group's. | Xian Yang Teng-Teng Man Qiao-Xia Tian Gui-Qiu Zhao Qing-Lan Kong Yan Meng Yan Gao Mei-Zhen Ning | 2014 | International Journal of Ophthalmology(English edition)2014，7，6： | 8 |
| 13 | Ultrasound screening of fetal structural abnormalities by standard ultrasound views during the first trimester显示文摘In China,ultrasound screening of fetal abnormalities is usually performed at 20 to 24 weeks of gestation.Recent data suggest that many fetal abnormalities can be detected as early as 11 to 14 weeks of gestation.1 In this study,we applied standard ultrasound views to screen for fetal structural abnormalities during the first trimester and investigated the effectiveness of the standard ultrasound views in the detection of fetal abnormalities. | WANG Li WU Qing-qing CHEN Yan MA Yu-qing YAO Ling LI Man | 2013 | Chinese Medical Journal2013，，5： | 7 |
| 14 | Surface free energy of copper-zinc alloy for energy-saving of boiler显示文摘Cu-Zn, Cu-Zn-Sn, Cu-Zn-Ni alloys were melted by vacuum smelter. The effect factors to the surface free energy of the alloys such as chemical composition, crystal structure and surface crystal lattice distortion etc. were investigated by OCA30 automatic contact angle test instrument, metallography microscope and XRD instrument etc. Results suggests: adding alloy element to Cu may increase its surface free energy, and the more kinds of alloy elements are added, the more surface free energy increases; the alloy element Sn an increase the surface free energy of Cu-Zn alloy; Cu-Zn alloy with fir-tree crystal structure, great phase discrepancy and obvious composition aliquation has greater surface free energy; Cu-Zn alloy with compounds and serious surface crystal lattice distortion has greater surface free energy. | WANG Man LIANG Jinsheng TANG Qingguo MING Xing MENG Junping DING Yan | 2006 | Rare Metals2006，25，z1： | 6 |
| 15 | Application of robotics in gastrointestinal endoscopy:Areview显示文摘Multiple robotic flexible endoscope platforms have been developed based on cross specialty collaboration between engineers and medical doctors. However, significant number of these platforms have been developed for the natural orifice transluminal endo-scopic surgery paradigm. Increasing amount of evidence suggest the focus of development should be placed on advanced endolumenal procedures such as endoscopic submucosal dissection instead. A thorough literature analysis was performed to assess the current status of robotic flexible endoscopic platforms designed for advanced endolumenal procedures. Current efforts are mainly focused on robotic locomotion and robotic instrument control. In the future, advances in actuation and servoing technology, optical analysis, augmented reality and wireless power transmission technology will no doubt further advance the field of robotic endoscopy. Globally, health systems have become increasingly budget conscious; widespread acceptance of robotic endoscopy will depend on careful design to ensure its delivery of a cost effective service. | Baldwin Po Man Yeung Philip Wai Yan Chiu | 2016 | World Journal of Gastroenterology2016，22，5： | 6 |
| 16 | MicroRNA-1304 suppresses human non-small cell lung cancer cell growth in vitro by targeting heme oxygenase-1显示文摘以前的研究证明了 microRNA-1304 (miR-1304 ) 是在癌症的某些类型的 dysregulated,包括非小的房间肺癌症(NSCLC ) ,并且可能涉及肿瘤幸存或生长。在这研究,我们在 vitro 在 NSCLC 调查了 miR-1304 和它的功能的直接目标。人的肺腺癌房间线(A549 和 NCI-H1975 ) 被学习。房间增长和幸存经由房间数, MTT 和殖民地形成试金被调查。房间 apoptosis 和房间周期用分别地染色试金的 annexin V-PE/7-AAD 和 PI 被检验。双酶的记者试金被用来由 miR-1304 验证 heme oxygenase-1 (HO-1 ) 的 post-transcriptional 规定。CRISPR/Cas9 被用来弄空内长的 miR-1304。MiR-1304 的 Overexpression 显著地减少了 NSCLC 房间和殖民地形成的数字和生存能力,和导致的房间 apoptosis 和 G 0/G1 阶段房间骑车拘捕。HO-1 被表明是在 NSCLC 房间的 miR-1304 的一个直接目标。由 hemin (20 mol/L ) 的 HO-1 表示的恢复在细胞生长上废除了 miR-1304 的抑制并且在 A549 细胞救了 miR-1304-induced apoptosis。有 anti-1304 的内长的 miR-1304 的抑制显著地增加了 HO-1 表示并且在 A549 房间支持了房间生长和幸存。在 17 件人的 NSCLC 织物样品, miR-1304 表示显著地被减少,当 HO-1 表示显著地作为与正常的肺纸巾相比被增加时。MicroRNA-1304 是肿瘤 suppressor, HO-1 是它在 NSCLC 的直接目标。结果为 NSCLC 作为一个治疗学的目标为 miR-1304 建议潜力。 | Cheng-gang LI Meng-fan PU Chun-zhu LI Man GAO Ming-xia LIU Cun-zhi YU Hong YAN Chun PENG Yang ZHAO Yu LI Ze-long MA Xin-ming QI Yi-zheng WANG Ling-ling MIAO Jin REN | 2017 | Acta Pharmacologica Sinica2017，38，1： | 6 |
| 17 | Pharmacokinetic study of single and multiple oral dose administration of antofloxacin hydrochloride in healthy male volunteers显示文摘背景 A 新 fluroquinolone 抗菌剂代理人, antofloxacin hydrochloride ,在中国发展了,学习的 levofloxacin.The 目的 8-NH2 derivant 是用汉语评估 antofloxacin hydrochloride 的单个、多重的口头的剂量的 pharmacokinetic 特征健康的男 volunteers.Methods 一开标签,非使随机化,单个、多重的剂量临床的试用是 conducted.In 单身者剂量学习, 12 个题目拿了 200 mg antofloxacin hydrochloride.In 多重剂量学习, 12 | LU Yuan KANG Zi-sheng ZHU Yan ZHANG Ming LIU Yan ZHANG Man LI Tian-yun XIAO Yong-hong | 2011 | Chinese Medical Journal2011，，2： | 6 |
| 18 | Epigenetic modification in histone deacetylase deletion strain of Calcarisporium arbuscula leads to diverse diterpenoids显示文摘Epigenetic modifications have been proved to be a powerful way to activate silent gene clusters and lead to diverse secondary metabolites in fungi. Previously, inactivation of a histone H3 deacetylase in Calcarisporium arbuscula had led to pleiotropic activation and overexpression of more than 75% of the biosynthetic genes and isolation of ten compounds. Further investigation of the crude extract of C. arbuscula Δhda A strain resulted in the isolation of twelve new diterpenoids including three cassanes(1-3), one cleistanthane(4), six pimaranes(5-10), and two isopimaranes(11 and 12) along with two know cleistanthane analogues. Their structures were elucidated by extensive NMR spectroscopic data analysis. Compounds 2 and 4 showed potent inhibitory effects on the expression of MMP1 and MMP2(matrix metalloproteinases family) in human breast cancer(MCF-7) cells. | Jian Bai Rong Mu Man Dou Daojiang Yan Bingyu Liu Qian Wei Jun Wan Yi Tang Youcai Hu | 2018 | Acta Pharmaceutica Sinica B2018，8，4： | 5 |
| 19 | Down-regulation of CEACAM1 in breast cancer显示文摘(CEACAM1 ) Carcinoembryonic 抗原相关的粘附分子 1 是一种类型属于 CEA 家庭的 1 transmembrane glycoprotein,它当在身体液体的也可溶的形式或房间上的膜界限形式出现,被发现了存在。异常 CEACAM1 表示与肿瘤前进被联系并且在许多人的恶意被发现了。增加的兴趣在乳癌被奉献给 CEACAM1 的表示,但是大多数这些调查结果是矛盾的。这研究的目的是详细地在乳癌调查 CEACAM1 表示。用染色的 immunohistochemical,我们发现那 CEACAM1 表情与 noncancerous 胸纸巾相比在乳癌纸巾被减少或输。另外,可溶的 CEACAM1 在乳癌房间线的文化媒介铺平是比在一个 nontumorigenic 乳房的那些显著地低的上皮的房间线。Immunofluorescence 分析一致地证明乳癌房间线有膜界限 CEACAM1 的相对低的表示。而且, CEACAM1 mRNA 和蛋白质表示层次在分别地用即时反向的 transcriptase 聚合酶链反应和西方的污点分析测量了的乳癌房间线是下面调整的。一起拿,我们的结果在乳癌表明 CEACAM1 的一条系统的下面规定并且建议恢复 CEACAM1 表示的策略可能对乳癌的治疗有用。 | Changcheng Yang Pingqing He Yiwen Liu Yiqing He Cuixia Yang Yan Du Muqing Zhou Wenjuan Wang Guoliang Zhang Man Wu Feng Gao | 2015 | Acta Biochimica et Biophysica Sinica2015，47，10： | 5 |
| 20 | Inorganic Elemental Determinations of Marine Traditional Chinese Medicine Meretricis concha from Jiaozhou Bay:The construction of Inorganic Elemental Fingerprint Based on Chemometric Analysis显示文摘The goal of this paper is to explore the relationship between the inorganic elemental fingerprint and the geographical origin identification of Meretricis concha,which is a commonly used marine traditional Chinese medicine(TCM) for the treatment of asthma and scald burns.For that,the inorganic elemental contents of Meretricis concha from five sampling points in Jiaozhou Bay have been determined by means of inductively coupled plasma optical emission spectrometry,and the comparative investigations based on the contents of 14 inorganic elements(Al,As,Cd,Co,Cr,Cu,Fe,Hg,Mn,Mo,Ni,Pb,Se and Zn) of the samples from Jiaozhou Bay and the previous reported Rushan Bay were performed.It has been found that the samples from the two bays are approximately classified into two kinds using hierarchical cluster analysis,and a four-factor model based on principle component analysis could explain approximately 75% of the detection data,also linear discriminant analysis can be used to develop a prediction model to distinguish the samples from Jiaozhou Bay and Rushan Bay with accuracy of about 93%.The results of the present investigation suggested that the inorganic elemental fingerprint based on the combination of the measured elemental content and chemometric analysis is a promising approach for verifying the geographical origin of Meretricis concha,and this strategy should be valuable for the authenticity discrimination of some marine TCM. | SHAO Mingying LI Xuejie ZHENG Kang JIANG Man YAN Cuiiwe LI Yantuan | 2016 | Journal of Ocean University of China2016，15，2： | 5 |
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