|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Engineering Broad-Spectrum Bacterial Blight Resistance by Simultaneously Disrupting Variable TALE-Binding Elements of Multiple Susceptibility Genes in Rice显示文摘Xanthomonas oryzae pv.oryzae(Xoo),the causal agent of bacterial blight of rice,employs the transcription activator-like effectors(TALEs)to induce the expression of the OsSWEET family of putative sugar transporter genes,which function in conferring disease susceptibility(S)in rice plants.To engineer broadspectrum bacterial blight resistance,we used CRISPR/Cas9-mediated gene editing to disrupt the TALEbinding elements(EBEs)of two S genes,OsSWEETH and OsSWEET14,in rice cv.Kitaake,which harbors the recessive resistance allele of Xa25/OsSWEET13.The engineered rice line MS14K exhibited broadspectrum resistance to most Xoo strains with a few exceptions,suggesting that the compatible strains may contain new TALEs.We identified two PthXo2-like TALEs,Tal5LN18 and Tal7PX061,as major virulence factors in the compatible Xoo strains LN18 and PX061,respectively,and found that Xoo encodes at least five types of PthXo2-like effectors.Given that PthXo2/PthXo2.1 target OsSlVEETf3 for transcriptional activation,the genomes of 3000 rice varieties were analyzed for EBE variationsin the OsSWEET13 promoter,and 10Xa25-like haplotypes were identified.We found that Tal5LN18 and Tal7PX〇6i bind slightly different EBE sequences in the OsSWEET13 promoter to activate its expression.CRISPR/Cas9 technology was then used to generate InDels in the EBE of the OsSWEET13 promoter in MS14K to creat a new germplasm with three edited OsSWEET EBEs and broad-spectrum resistance against all Xoo strains tested.Collectively,our findings illustrate how to disarm TALE-S co-evolved loci to generate broad-spectrum resistance through the loss of effector-triggered susceptibility in plants. | Zhengyin Xu Xiameng Xu Qiang Gong Ziyang Li Ying Li Sai Wang Yangyang Yang Wenxiu Ma Longyu Liu Bo Zhu Lifang Zou Gongyou Chen | 2019 | Molecular Plant2019，12，11： | 29 |
| 2 | Regeneration performance and carbon consumption of semi-coke and activated coke for SO_2 and NO removal显示文摘To decrease the operating cost of flue gas purification technologies based on carbon-based materials, the adsorption and regeneration performance of low-price semi-coke and activated coke were compared for SO2 and NO removal in a simulated flue gas. The functional groups of the two adsorbents before and after regeneration were characterized by a Fourier transform infrared(FTIR) spectrometer, and were quantitatively assessed using temperature programmed desorption(TPD) coupled with FTIR and acid–base titration. The results show that semi-coke had higher adsorption capacity(16.2% for SO2 and 38.6% for NO) than activated coke because of its higher content of basic functional groups and lactones. After regeneration, the adsorption performance of semi-coke decreased because the number of active functional groups decreased and the micropores increased. Semi-coke had better regeneration performance than activated coke. Semi-coke had a larger SO2 recovery of 7.2% and smaller carbon consumption of 12% compared to activated coke. The semi-coke carbon-based adsorbent could be regenerated at lower temperatures to depress the carbon consumption, because the SO2 recovery was only reduced a small amount. | Song Ding Yuran Li Tingyu Zhu Yangyang Guo | 2015 | Journal of Environmental Sciences2015，27，8： | 17 |
| 3 | Adsorption and desorption of SO_2, NO and chlorobenzene on activated carbon显示文摘Activated carbon(AC) is very effective for multi-pollutant removal; however, the complicated components in flue gas can influence each other's adsorption. A series of adsorption experiments for multicomponents, including SO_2, NO, chlorobenzene and H2 O,on AC were performed in a fixed-bed reactor. For single-component adsorption, the adsorption amount for chlorobenzene was larger than for SO_2 and NO on the AC. In the multi-component atmosphere, the adsorption amount decreased by 27.6% for chlorobenzene and decreased by 95.6% for NO, whereas it increased by a factor of two for SO_2,demonstrating that a complex atmosphere is unfavorable for chlorobenzene adsorption and inhibits NO adsorption. In contrast, it is very beneficial for SO_2 adsorption. The temperature-programmed desorption(TPD) results indicated that the binding strength between the gas adsorbates and the AC follows the order of SO_2> chlorobenzene > NO. The adsorption amount is independent of the binding strength. The presence of H2 O enhanced the component effects, while it weakened the binding force between the gas adsorbates and the AC. AC oxygen functional groups were analyzed using TPD and X-ray photoelectron spectroscopy(XPS) measurements. The results reveal the reason why the chlorobenzene adsorption is less affected by the presence of other components. Lactone groups partly transform into carbonyl and quinone groups after chlorobenzene desorption. The chlorobenzene adsorption increases the number of C = O groups, which explains the positive effect of chlorobenzene on SO_2 adsorption and the strong NO adsorption. | Yuran Li Yangyang Guo Tingyu Zhu Song Ding | 2016 | Journal of Environmental Sciences2016，28，5： | 8 |
| 4 | Ultra-sensitive graphene strain sensor for sound signal acquisition and recognition显示文摘为健全信号获得和识别的一个便携、高精确的传感器从编织的特殊设计的 graphene 的薄电影被制作织物(GWF ) 。在被拉长之上,随机的裂缝的高密度出现在网络,它减少当前的小径,从而增加电阻。因此,这部电影能对人的喉咙作为一个紧张传感器起作用以便测量通过肌肉运动,一个声音是否被生产的讲话。超离频敏感允许由提取声波的签名特征的快速、低频率的讲话采样的实现。在这研究, 26 封英语信,典型汉字和音调的代表性的信号,甚至短语和句子被测试,揭示在电阻的明显、典型的变化。而且, graphene 传感器的电阻变化与预告录下的声音完美地反应了。由把人工智能与数字信号处理相结合,我们期望以后,这个 graphene 传感器将能成功地达成复杂声学的系统和听觉的数据的大数量。 | Yan Wang Tingting Yang Junchao Lao Rujing Zhang Yangyang Zhang Miao Zhu Xiao Li Xiaobei Zang Kunlin Wang Wenjian YU Hu Jin Li Wang Hongwei Zhu | 2015 | Nano Research2015，8，5： | 8 |
| 5 | Correlation between tobacco and alcohol use and Traditional Chinese Medicine constitutions: an analysis based on a sample from general population of China显示文摘OBJECTIVE:To investigate the relationship between smoking and drinking and constitution types from Traditional Chinese Medicine(TCM)theory.METHODS:The Constitution in Chinese Medicine Questionnaire(CCMQ)was used.A sample of 8448subjects from nine provinces and municipalities was chosen from the database of the TCM constitution and health status survey.Constituent ratio and correspondence analysis were used to find the relationship between smoking and drinking and TCM constitution.RESULTS:There was an association between smokinganddrinkingandtheGentleness,phlegm-dampness,and dampness-heat type constitutions.People that did not smoke tobacco or drink alcohol tended to have Yang-deficiency,Yin-deficiency,Qi-depression,Qi-deficiency,special diathesis,orblood-stasis type constitutions.CONCLUSION:There was a significant correlation between tobacco and alcohol use and TCM constitutions.To improve patient health,more attention should be paid to tobacco and alcohol control. | Yangyang Wang Yanbo Zhu Jie Di Lin Lin | 2013 | Journal of Traditional Chinese Medicine2013，33，5： | 7 |
| 6 | Case-control study on the associations between lifestyle-behavioral risk factors and phlegm-wetness cbonstitution显示文摘OBJECTIVE: To explore the relationships between different lifestyle-behavioral factors and phlegm wetness type of Traditional Chinese Medicine constitution,so as to provide health management strategies for phlegm-wetness constitution.METHODS: A case-control study was conducted with the cases selected from the database of Chinese constitution survey in 9 provinces or municipalities of China. 1380 cases met the diagnostic criteria of phlegm-wetness type were taken as the case group, and 1380 cases were randomly selected from gentleness type as the control group. Using Chi-square test to compare the differences of lifestyle-behavior composition in each group; single factor and multiple logistic regression analysis were used to compare the relationships of life-style-behavioral factors and phlegm-wetness type.RESULTS: There were statistically significant differences between phlegm-wetness type group and gentleness type group in lifestyle behaviors(dietary habits, tobacco and liquor consumptions, exercise habits, sleeping habits). The results of single factor logistic regression analysis demonstrated that the risk of phlegm-wetness constitution decreased significantly in light diet(odds ratio, OR=0.68);The risk factors of phlegm-wetness type were fatty food intake(OR=2.36), sleeping early and getting up late(OR=1.87), tobacco smoking(OR=1.83),barbecued food intake(OR=1.68), alcohol drinking(OR=1.63), salty food intake(OR=1.44), sleeping erratically(OR=1.43), less physical activities(OR=1.42), sweet food intake(OR=1.29), sleeping and getting up late(OR=1.26), and pungent food intake(OR=1.21), respectively. Regardless of the interaction among lifestyle-behavioral factors, the results of the multiple logistic regression analysis revealed that the risk factors of phlegm-wetness type were sleeping early and getting up late(OR=1.94), fatty food intake(OR=1.80), tobacco smoking(OR=1.50),sleeping erratically(OR=1.50), barbecued food intake(OR=1.40), sleeping and getting up late(OR=1.40), less physical activities(OR=1.31), sleeping late and getting up early(OR=1.27),and sweet food intake(OR=1.27), respectively, and the risk ofphlegm-wetness type still decreased significantly inlightfoodintake(OR=0.79).CONCLUSION: Light diet can decrease the risk of being phlegm-wetness constitution, and bad lifestyle behaviors such as sleeping early and getting up late, sleeping erratically, fatty food, barbecued food or sweet food intake, tobacco and liquor consumptions, and less physical activities can increase the risks of becoming phlegm-wetness constitution. | Yanbo Zhu Qi Wang Zhaoyu Dai Hideki Origasa Jie Di Yangyang Wang Lin Lin Chunpok Fan | 2014 | Journal of Traditional Chinese Medicine2014，34，3： | 6 |
| 7 | Clopidogrel improves aspirin response after off-pump coronary artery bypass surgery显示文摘We sought to assess the incidence of aspirin resistance after off-pump coronary artery bypass(OPCAB) surgery,and investigate whether clopidogrel can improve aspirin response and be safely applied early after OPCAB surgery.Sixty patients who underwent standard OPCAB surgery were randomized into two groups.One group(30 patients)received mono-antiplatelet treatment(MAPT) with aspirin 100 mg daily and the other group received dual antiplatelet treatment(DAPT) with aspirin 100 mg daily plus clopidogrel 75 mg daily.Platelet aggregations in response to arachidonic acid(PL_(AA)) and adenosine diphosphate(ADP)(PL_(ADP)) were measured preoperatively and on days 1 to 6,8 and10 after the antiplatelet agents were administered.A PL_(AA) level above 20%was defined as aspirin resistance.Postoperative bleeding and other perioperative variables were also recorded.There were no significant differences between the two groups in baseline characteristics,average number of distal anastomosis,operation time,postoperative bleeding,ventilation time and postoperative hospital stay.However,the incidence of aspirin resistance was significantly lower in the DAPT group than that in the MAPT group on the first and second day after antiplatelet agents were given(62.1%vs.32.1%,34.5%vs.10.7%,respectively,both P < 0.05).There was no significant difference in postoperative complication between the two groups.DAPT with aspirin and clopidogrel can be safely applied to OPCAB patients early after the procedure.Moreover,clopidogrel reduces the incidence of OPCAB-related aspirin resistance. | Xuezhong Wang Xiaoxuan Gong Tiantian Zhu Qiu Zhang Yangyang Zhang Xiaowei Wang Zhijian Yang Chunjian Lia | 2014 | The Journal of Biomedical Research2014，28，2： | 5 |
| 8 | Kinetics and mechanisms of mitotic inheritance of DNA methylation and their roles in aging-associated methylome deterioration显示文摘Mitotic inheritance of the DNA methylome is a challenging task for the maintenance of cell identity.Whether DNA methylation pattern in different genomic contexts can all be faithfully maintained is an open question.A replication-coupled DNA methylation maintenance model was proposed decades ago,but some observations suggest that a replication-uncoupled maintenance mechanism exists.However,the capacity and the underlying molecular events of replication-uncoupled maintenance are unclear.By measuring maintenance kinetics at the single-molecule level and assessing mutant cells with perturbation of various mechanisms,we found that the kinetics of replication-coupled maintenance are governed by the UHRF1–Ligase 1 and PCNA–DNMT1 interactions,whereas nucleosome occupancy and the interaction between UHRF1 and methylated H3K9 specifically regulate replication-uncoupled maintenance.Surprisingly,replication-uncoupled maintenance is sufficiently robust to largely restore the methylome when replication-coupled maintenance is severely impaired.However,solo-WCGW sites and other CpG sites displaying aging-and cancer-associated hypomethylation exhibit low maintenance efficiency,suggesting that although quite robust,mitotic inheritance of methylation is imperfect and that this imperfection may contribute to selective hypomethylation during aging and tumorigenesis. | Xuan Ming Zhuqiang Zhang Zhuoning Zou Cong Lv Qiang Dong Qixiang He Yangyang Yi Yingfeng Li Hailin Wang Bing Zhu | 2020 | Cell Research2020，30，11： | 4 |
| 9 | Low-temperature sintered(Na_(1/2)Bi_(1/2))TiO_(3)-based incipient piezoceramics for co-fired multilayer actuator application显示文摘Low-temperature sintered(Na_(1/2)Bi_(1/2))_(0.935)Ba_(0.065)Ti_(0.975)(Fe_(1/2)Nb_(1/2))_(0.025)O_(3)(NBT-BT-0.025FN)lead-free incipient piezoceramics were investigated using high-purity Li_(2)CO_(3) as sintering aids.With the ≤0.5 wt%Li_(2)CO_(3) addition,the introduced Li^(+) cations precede to enter the A-sites of the perovskite lattice to compensate for the A-site deficiencies.Once the addition exceeds 0.5 wt%,the excess Lit cations will occupy B-sites and give rise to the generation of oxygen vacancies,which accelerate the mass transport and thus lower the sintering temperature effectively from 1100℃ down to 925℃.It was also found that a small amount of Lit addition has little effect on the phase structure and electromechanical properties of the system,but overweight seriously disturbs these characteristics because of the large lattice distortion.The sintered NBT-BT-0.025FN incipient piezoceramics with 1.25 wt%Li_(2)CO_(3) addition at 925℃ provides a large strain of 0.33% and a corresponding large signal piezoelectric coefficient d_(33)^(*) of 550 pm/V at 60 kV/cm,indicating this system is a very promising candidate for lead-free co-fired multilayer actuator application. | Pengyuan Fan Yangyang Zhang Shan-Tao Zhang Bing Xie Yiwei Zhu Mohsin Ali Marwat Weigang Ma Kai Liu Liang Shu Haibo Zhang | 2019 | Journal of Materiomics2019，5，3： | 3 |
| 10 | AGRICULTURAL GREEN DEVELOPMENT TO ACHIEVE FOOD SECURITY AND CARBON REDUCTION IN THE CONTEXT OF CHINA'S DUAL CARBON GOALS显示文摘The agricultural sector,a major source of greenhouse gas emissions,and emissions from agriculture must be reduced substantially to achieve carbon(C)neutrality.Based on a literature analysis and other research results,this study investigated the effects and prospects of C reduction in agricultural systems under different scenarios(i.e.,methods and approaches)in the context of China's dual C goals,as those working in the agricultural sector have yet to reach a consensus on how to move forward.Different views,standards,and countermeasures were analyzed to provide a reference for agricultural action supporting China's C neutrality goal. | Yulong YIN Kai HE Zhong CHEN Yangyang LI Fengling REN Zihan WANG Yingcheng WANG Haiqing GONG Qichao ZHU Jianbo SHEN Xuejun LIU Zhenling CUI | 2023 | Frontiers of Agricultural Science and Engineering2023，10，2： | 2 |
| 11 | A 0.19 ppm/°C bandgap reference circuit with high-PSRR显示文摘A high-order curvature-compensated CMOS bandgap reference(BGR) topology with a low temperature coefficient(TC) over a wide temperature range and a high power supply reject ratio(PSRR) is presented.High-order correction is realized by incorporating a nonlinear current INL, which is generated by ?V_(GS) across resistor into current generated by a conventional first-order current-mode BGR circuit. In order to achieve a high PSRR over a broad frequency range, a voltage pre-regulating technique is applied. The circuit was implemented in CSMC 0.5 μm 600 V BCD process. The experimental results indicate that the proposed topology achieves TC of0.19 ppm/°C over the temperature range of 165 °C(-40 to 125 °C), PSRR of-123 d B @ DC and-56 d B @ 100 k Hz. In addition, it achieves a line regulation performance of 0.017%/V in the supply range of 2.8–20 V. | Jing Leng Yangyang Lu Yunwu Zhang Huan Xu Kongsheng Hu Zhicheng Yu Weifeng Sun Jing Zhu | 2018 | Journal of Semiconductors2018，39，9： | 2 |
| 12 | Author Correction: Kinetics and mechanisms of mitotic inheritance of DNA methylation and their roles in aging-associated methylome deterioration显示文摘We apologize for the mistake of the fund information in the acknowledgements.The correct information is as follows.This correction does not affect the conclusion of the work. | Xuan Ming Zhuqiang Zhang Zhuoning Zou Cong Lv Qiang Dong Qixiang He Yangyang Yi Yingfeng Li Hailin Wang Bing Zhu | 2021 | Cell Research2021，31，3： | 2 |
| 13 | Study on the optimization of the combined blown converter process in Chongqing Iron and Steel Company显示文摘The effects of lance height and bottom blown flowrate on the mixed time,the splashing amount,the penetrating depth,and the level fluctuation of an 85 t combined blown converter have been studied using a water model.The results show that the maximal stirring energy is provided to the bath at the top lance height of about 50-100 mm.When the top lance height is in the range of 90- 110 mm,the splashing amount caused by the top jet can reach the maximal value.The appropriate operational parameters of Chongqing Iron and Steel Company(CISC)converter have been established that the top lance height is 1600-1760 mm and the bot- tom blowing flowrate is 240-480 Nm^3/h in the primary phase of a heat,1100-1300 mm and 160-200 Nm^3/h in the second phase,and 1040-1120 mm and 200-350 Nm^3/h in the end phase.Also,the trial shows that the metallurgical result of the studied blow pattern is better than that of the former pattern.At the starting 3-4 min of a heat,the strong splashing is eliminated. | Ping Tang Yangyang Yu Guanghua Wen Mingmei Zhu Liang Zhou Yiju Long Qing Liang | 2008 | Journal of University of Science and Technology Beijing2008，15，1： | 2 |
| 14 | Interaction between curcumin and mimetic biomembrane显示文摘Curcumin,a major bioactive compound in turmeric,has a broad spectrum of antioxidant,anticarcinogenic,antimutagenic and anti-inflammatory properties.At the molecular level,curcumin modulates many structurally unrelated membrane proteins through several signaling pathways.Curcumin has been suggested to change the properties of cell membranes and affect the membrane-bound proteins indirectly;however,the detailed mechanism has yet to be investigated.In this paper,self-assembled bilayer lipid membranes are artificially constructed on the surface of a gold electrode to mimic biomembranes,and interaction between the supported membranes and curcumin is studied electrochemically.Results show that curcumin interacts with the membranes strongly,in a concentration-dependent manner.At low concentrations,curcumin tends to insert into the outer monolayer only,while at high concentrations,it may also begin to penetrate the inner monolayer.The results obtained in this work may enhance our understanding of the effect of curcumin,and possibly flavonoids,on cell membranes and membrane proteins. | CHEN GuiFang CHEN YangYang YANG NaNa ZHU XueJun SUN LiZhou LI GenXi | 2012 | Science China(Life Sciences)2012，55，6： | 2 |
| 15 | Identification of sources,characteristics and photochemical transformations of dissolved organic matter with EEM-PARAFAC in the Wei River of China显示文摘Dissolved organic matter(DOM)in rivers is a critical regulator of the cycling and toxicity of pollutants and the behavior of DOM is a key indicator for the health of the environment.We investigated the sources and characteristics of DOM in surface water and sediment samples of the Wei River,China.Dissolved organic carbon(DOC)concentration and ultraviolet absorbance at 254 nm(UV254)increased in the surface water and were decreased in the sediment downstream,indicating that the source of DOM in the water differed from the sediment.Parallel factor(PARAFAC)analysis of the excitation-emission matrices(EEM)revealed the presence of terrestrial humus-like,microbial humus-like and tryptophan-like proteins in the surface water,whereas the sediment contained UVA humic-like,UVC humic-like and fulvic-like in the sediment.The DOM in the surface water and sediment were mainly derived from microbial metabolic activity and the surrounding soil.Surface water DOM displayed greater photodegradation potential than sediment DOM.PARAFAC analysis indicated that the terrestrial humic-like substance in the water and the fulvic-like component in the sediment decomposed more rapidly.These data describe the characteristics of DOM in the Wei River and are crucial to understanding the fluctuations in environmental patterns. | Yuanyuan Luo Yangyang Zhang Mengfan Lang Xuetao Guo Tianjiao Xia Tiecheng Wang Hanzhong Jia Lingyan Zhu | 2021 | Frontiers of Environmental Science & Engineering2021，15，5： | 2 |
| 16 | Deep-learning cell imaging through Anderson localizing optical fiber显示文摘We demonstrate a deep-learning-based fiber imaging system that can transfer real-time artifact-free cell images through a meter-long Anderson localizing optical fiber.The cell samples are illuminated by an incoherent LED light source.A deep convolutional neural network is applied to the image reconstruction process.The network training uses data generated by a setup with straight fiber at room temperature(∼20°C)but can be utilized directly for high-fidelity reconstruction of cell images that are transported through fiber with a few degrees bend or fiber with segments heated up to 50°C.In addition,cell images located several millimeters away from the bare fiber end can be transported and recovered successfully without the assistance of distal optics.We provide evidence that the trained neural network is able to transfer its learning to recover images of cells featuring very different morphologies and classes that are never“seen”during the training process. | Jian Zhao Yangyang Sun Hongbo Zhu Zheyuan Zhu Jose E.Antonio-Lopez Rodrigo Amezcua Correa Shuo Pang Axel Schulzgen | 2019 | Advanced Photonics2019，1，6： | 2 |
| 17 | SPM-IS: An auto-algorithm to acquire a mature soybean phenotype based on instance segmentation显示文摘Mature soybean phenotyping is an important process in soybean breeding;however, the manual process is time-consuming and labor-intensive. Therefore, a novel approach that is rapid, accurate and highly precise is required to obtain the phenotypic data of soybean stems, pods and seeds. In this research, we propose a mature soybean phenotype measurement algorithm called Soybean Phenotype Measure-instance Segmentation(SPM-IS). SPM-IS is based on a feature pyramid network, Principal Component Analysis(PCA) and instance segmentation. We also propose a new method that uses PCA to locate and measure the length and width of a target object via image instance segmentation. After 60,000 iterations, the maximum mean Average Precision(m AP) of the mask and box was able to reach 95.7%. The correlation coefficients R^(2) of the manual measurement and SPM-IS measurement of the pod length, pod width, stem length, complete main stem length, seed length and seed width were 0.9755, 0.9872, 0.9692, 0.9803,0.9656, and 0.9716, respectively. The correlation coefficients R^(2) of the manual counting and SPM-IS counting of pods, stems and seeds were 0.9733, 0.9872, and 0.9851, respectively. The above results show that SPM-IS is a robust measurement and counting algorithm that can reduce labor intensity, improve efficiency and speed up the soybean breeding process. | Shuai Li Zhuangzhuang Yan Yixin Guo Xiaoyan Su Yangyang Cao Bofeng Jiang Fei Yang Zhanguo Zhang Dawei Xin Qingshan Chen Rongsheng Zhu | 2022 | The Crop Journal2022，10，5： | 2 |
| 18 | miR-34a inhibits the migration and invasion of esophageal squamous cellcarcinoma by targeting Yin Yang-1显示文摘 | Jihua Nie Xin Ge Yangyang Geng Han Cao Wei Zhu Yang Jiao Jinchang Wu Jundong Zhou Jianping Cao | 2015 | Oncology Reports2015，，： | 1 |
| 19 | Fabrication of high-aspect-ratio electrode array by combining UV-LIGA with micro electro-discharge machining 显示文摘 | Hu Yangyang Zhu Di Qu Ningsong | 2009 | Microsyst Technol2009，15，： | 1 |
| 20 | Simultaneous determination of four Sudan dyes in rat blood by UFLC–MS/MS and its application to a pharmacokinetic study in rats显示文摘A rapid and sensitive method based on ultrafast liquid chromatography–tandem mass spectrometry was developed and validated for simultaneous determination of Sudan I, Sudan II, Sudan III, and Sudan IV levels in rat whole blood. Cleanert C18 mixed-mode polymeric sorbent was used for effective solid-phase extraction cleanup. Separation was carried out on a reversed-phase C18column(100 mm 2.1 mm,1.8 μm) using 0.1%(v/v) formic acid in water/0.1%(v/v) formic acid in acetonitrile as the mobile phase in gradient elution. Quanti fi cation was performed by an electrospray ionization source in the positive multiple reaction monitoring mode using D5-Sudan I as the internal standard. Calibration curves showed good linearity between 0.2 and 20.0 μg/L, with correlation coef fi cients higher than 0.9990. The average recovery rates were between 93.05% and 114.98%. The intra- and inter-day relative standard deviations were within 6.2%. The lower limit of quanti fi cation was 0.2 μg/L. All the analytes were found to be stable in a series of stability studies. The proposed method was successfully applied to a pharmacokinetic study of four Sudan dyes after oral administration to rats. | Hao Zhu Yijun Chen Changshun Huang Yangyang Han Yiwei Zhang Shucan Xie Xiaohong Chen Micong Jin | 2015 | Journal of Pharmaceutical Analysis2015，5，4： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员