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| 1 | 5-Hydroxymethylcytosine signatures in circulating cell-free DNA as diagnostic biomarkers for human cancers显示文摘象 5-methylcytosine (5mC ) 和 5-hydroxymethylcytosine (5hmC ) 那样的 DNA 修正是知道在哺乳动物影响全球基因表示的 epigenetic 标记。在人的染色体,与基因表示的靠近的关联和高化学的稳定性给他们的流行,这些 DNA epigenetic 标记能为癌症用作理想的 biomarkers 诊断。利用一种高度敏感、选择的化学标记技术,我们这里报导在传播没有房间的 DNA ( cfDNA )并且在从最近与 colorectal 诊断的 260 个病人的一个队收集的配对的肿瘤和邻近的纸巾的 genomic DNA ( gDNA )的 5hmC 的染色体宽的介绍,胃,胰腺,从 90 个健康个人的肝或甲状腺癌症和正常纸巾。5hmC 主要在与开的染色质和容许的 histone 修正重合的 transcriptionally 活跃的区域被散布。柔韧的联系癌症的 5hmC 签名在 cfDNA 被识别为特定的癌症类型是特征的。传播 cfDNA 的 5hmC 底 biomarkers colorectal 和胃的癌症是高度预兆的并且比常规 biomarkers 优异并且比得上从织物活体检视的 5hmC biomarkers。因此,这新策略能从血样品的分析为癌症的诊断和预后导致有效、最低限度地侵略的方法的发展。 | Wenshuai Li Xu Zhang Xingyu Lu Lei You Yanqun Song Zhongguang Luo Jun Zhang Ji Nie Wanwei Zheng Diannan Xu Yaping Wang Yuanqiang Dong Shulin Yu Jun Hong Jianping Shi Hankun Hao Fen Luo Luchun Hua Peng Wang Xiaoping Qian Fang Yuan Lianhuan Wei Ming Cui Taiping Zhang Quan Liao Menghua Dai Ziwen Liu Ge Chen Katherine Meckel Sarbani Adhikari Guifang Jia Marc B Bissonnette Xinxiang Zhang Yupei Zhao Wei Zhang Chuan He Jie Liu | 2017 | Cell Research2017，27，10： | 30 |
| 2 | Segmentation of Hematoxylin-Eosin stained breast cancer histopathological images based on pixel-wise SVM classifier显示文摘Hematoxylin-Eosin(HE) staining is the routine diagnostic method for breast cancer(BC), and large amounts of HE stained histopathological images are available for analysis. It is emergent to develop computational methods to efficiently and objectively analyze these images, with the aim of providing potentially better diagnostic and prognostic information for BC. This work focuses on analyzing our in-house HE stained histopathological images of breast cancer tissues. Since tumor nests(TNs) and stroma morphological characteristics can reflect the biological behaviors of breast invasive ductal carcinoma(IDC), accurate segmentation of TNs and the stroma is the first step towards the subsequent quantitative analysis. We first propose a method based on the pixel-wise support vector machine(SVM) classifier for segmenting TNs and the stroma, then extract four morphological characters related to the TNs from the images and investigate their relationships with the patients' 8-year disease free survival(8-DFS). The evaluation result shows that the classification based segmentation method is able to distinguish between TNs and stroma with 87.1% accuracy and 80.2% precision,suggesting that the proposed method is promising in segmenting HE stained IDC histopathological images. The Kaplan-Meier survival curves show that three morphological characters(number of TNs, total perimeter, and average area of TNs) in the images have statistical correlations with 8-DFS of the patients, illustrating that the segmented images can help to identify new morphological factors in IDC TNs for the prediction of BC prognosis. | QU AiPing CHEN JiaMei WANG LinWei YUAN JingPing YANG Fang XIANG QingMing MASKEY Ninu YANG GuiFang LIU Juan LI Yan | 2015 | Science China(Information Sciences)2015，58，9： | 12 |
| 3 | Inflammation and tumor progression:signaling pathways and targeted intervention显示文摘Cancer development and its response to therapy are regulated by inflammation,which either promotes or suppresses tumor progression,potentially displaying opposing effects on therapeutic outcomes.Chronic inflammation facilitates tumor progression and treatment resistance,whereas induction of acute inflammatory reactions often stimulates the maturation of dendritic cells(DCs)and antigen presentation,leading to anti-tumor immune responses.In addition,multiple signaling pathways,such as nuclear factor kappa B(NF-kB),Janus kinase/signal transducers and activators of transcription(JAK-STAT),toll-like receptor(TLR)pathways,cGAS/STING,and mitogen-activated protein kinase(MAPK);inflammatory factors,including cytokines(e.g.,interleukin(IL),interferon(IFN),and tumor necrosis factor(TNF)-a),chemokines(e.g.,C-C motif chemokine ligands(CCLs)and C-X-C motif chemokine ligands(CXCLs)),growth factors(e.g.,vascular endothelial growth factor(VEGF),transforming growth factor(TGF)-P),and inflammasome;as well as inflammatory metabolites including prostaglandins,leukotrienes,thromboxane,and specialized proresolving mediators(SPM),have been identified as pivotal regulators of the initiation and resolution of inflammation.Nowadays,local irradiation,recombinant cytokines,neutralizing antibodies,small-molecule inhibitors,DC vaccines,oncolytic viruses,TLR agonists,and SPM have been developed to specifically modulate inflammation in cancer therapy,with some of these factors already undergoing clinical trials.Herein,we discuss the initiation and resolution of inflammation,the crosstalk between tumor development and inflammatory processes.We also highlight potential targets for harnessing inflammation in the treatment of cancer. | Huakan Zhao Lei Wu Guifang Yan Yu Chen Mingyue Zhou Yongzhong Wu Yongsheng Li | 2021 | Signal Transduction and Targeted Therapy2021，6，8： | 11 |
| 4 | MiRNA-203 suppresses tumor cell proliferation, migration and invasion by targeting Slug in gastric cancer显示文摘 | Liuqing Yang Hongwei Liang Yanbo Wang Shanting Gao Kai Yin Zhijian Liu Xi Zheng Ying Lv Lei Wang Chen-Yu Zhang Xi Chen Guifang Xu Weijie Zhang Xiaoping Zou | 2016 | Protein & Cell2016，7，5： | 7 |
| 5 | Expression of human soluble TRAIL in Chlamydomonas reinhardtii chloroplast显示文摘Tumor necrosis factor-related apop-tosis-inducing ligand (TRAIL) induces selectively apoptosis in various tumor cells and virus-infected cells, but rarely in normal cells. A chloroplast expres-sion vector, p64TRAIL, containing the cDNA coding for the soluble TRAIL (sTRAIL), was constructed with clpP-trnL-petB-chlL-rpl23-rpl2 as Chlamydomonas reinhardtii plastid homologous recombinant frag-ments and spectinomycin-resistant aadA gene as a select marker. The plasmid p64TRAIL was trans-ferred into the chloroplast genome of C. reinhardtii by the biolistic method. Three independently trans-formed lines were obtained by 100 mg/L spectino-mycin selection. PCR amplification, Southern blot analysis of the sTRAIL coding region DNA and culti-vation cells in the dark all showed that the exogenous DNA had been integrated into chloroplast genome of C. reinhardtii. Western blot analysis showed that human soluble TRAIL was expressed in C. reinhardtii chloroplast. The densitometric analysis of Western blot indicated that the expressed human sTRAIL protein in the chloroplasts of C. reinhardtii accounted for about 0.43%―0.67% of the total soluble proteins. These experimental results demonstrated the possi-bility of using transgenic chloroplasts of green alga as bioreactors for production of biopharmaceuticals. | YANG Zongqi LI yinü CHEN Feng LI Dong ZHANG Zhifang LIU Yanxin ZHENG Dexian WANG Yong SHEN Guifang | 2006 | Chinese Science Bulletin2006，51，14： | 4 |
| 6 | Underwater Laser Welding/Cladding for High-performance Repair of Marine Metal Materials:A Review显示文摘With the rapid developments of marine resource exploitation,mounts of marine engineering equipment are settled on the ocean.When it is not possible to move the damaged equipment into a dry dock,welding operations must be performed in underwater environments.The underwater laser welding/cladding technique is a promising and advanced technique which could be widely applied to the maintenance of the damaged equipment.The present review paper aims to present a critical analysis and engineering overview of the underwater laser welding/cladding technique.First,we elaborated recent advances and key issues of drainage nozzles all over the world.Next,we presented the underwater laser processing and microstructural-mechanical behavior of repaired marine materials.Then,the newly developed powder-feeding based and wire-feeding based underwater laser direct metal deposition techniques were reviewed.The differences between the convection,conduction,and the metallurgical kinetics in the melt pools during underwater laser direct metal deposition and in-air laser direct metal deposition were illustrated.After that,several challenges that need to be overcame to achieve the full potential of the underwater laser welding/cladding technique are proposed.Finally,suggestions for future directions to aid the development of underwater laser welding/cladding technology and underwater metallurgical theory are provided.The present review will not only enrich the knowledge in the underwater repair technology,but also provide important guidance for the potential applications of the technology on the marine engineering. | Guifang Sun Zhandong Wang Yi Lu Mingzhi Chen Kun Yang Zhonghua Ni | 2022 | Chinese Journal of Mechanical Engineering2022，35，1： | 4 |
| 7 | High flux photocatalytic self-cleaning nanosheet C_(3)N_(4) membrane supported by cellulose nanofibers for dye wastewater purification显示文摘Hazardous dye substances discharged from the textile and dyestuff industries not only threaten local the surrounding ecosystems but are also hard to degraded.We report the preparation of process for a photocatalytic membrane device that can degrade dye pollution under visible light.This filtration membrane,with a well-organized multilayer structure,simultaneously achieves continuous and flow-through separation of degradation products.Cellulose nanofibers(CNFs)were used as a template for nanosheet C_(3)N_(4)(NS C_(3)N_(4))preparation;the performance for the photocatalytic degradation of dyes improved as the morphology changed from bulking to nanosheet.NS C3N4 was then attached to the surface of a prepared CNF membrane via vacuum filtration.This device exhibited high efficiency(the degradation rates of both Rhodamine B and Methylene blue both reached 96%),high flux(above 160 L·h^(-1)·m^(-2)·bar^(-1))and excellent stability(maintaining steady flux and high separation were maintained after 4 h).This easy-preparation,easy-scale-up,and low-cost process provides a new method of fabricating photocatalytic membrane devices for dye wastewater treatment. | Lilong Zhang Ge Meng Guifang Fan Keli Chen Yulong Wu Jian Liu | 2021 | Nano Research2021，14，8： | 3 |
| 8 | Initial neutrophil lymphocyte ratio is superior to platelet lymphocyte ratio as an adverse prognostic and predictive factor in metastatic colorectal cancer显示文摘 | Wenzhuo He Chenxi Yin Guifang Guo Chang Jiang Fang Wang Huijuan Qiu Xuxian Chen Ruming Rong Bei Zhang Liangping Xia | 2013 | Medical Oncology2013，，1： | 2 |
| 9 | Fabrication of nanozyme@DNA hydrogel and its application in biomedical analysis显示文摘Nanozymes 由于容易的准备和低费用的优点收到了大注意。不同于乐意地适应生理的环境的自然的酶,人工的 nanozymes 是容易的在复杂临床的样品使钝化(例如,浆液) ,它可以损坏催化能力并且因而在生物医学的分析限制申请。为了征服这个问题,在这研究,我们由把 nanozymes 合并到纯 DNA hydrogel 制作了新奇 nanozyme@DNA hydrogel 建筑学。金 nanoparticles (AuNPs ) 作为模型 nanozyme 被采用。当他们被 hydrogel 保护,结果显示在 DNA hydrogel 合并的 AuNPs 在浆液保留他们的催化能力,而 AuNPs 完全独自在浆液失去催化能力。在浆液的氢过氧化物和葡萄糖的察觉基于 AuNPs@DNA hydrogel 的催化作用被完成。分别地,各个的察觉限制到达 1.7 和 38 M 它等于用自然的酶获得的价值。除机制以外,一些另外的优点例如再循环能力和可获得性,也被探索了。这 nanozyme@DNA hydrogel 体系结构可以在复杂生理的样品为生物医学的分析象 nanozymes 的申请一样为 nanozymes 的利用有一个大潜力。 | Xiaoli Zhu Xiaoxia Mao Zihan Wang Chang Feng Guifang Chen Genxi Li | 2017 | Nano Research2017，10，3： | 2 |
| 10 | Proton pump inhibitor pantoprazole abrogates adriamycin-resistant gastric cancer cell invasiveness via suppression of Akt/GSK-β/β-catenin signaling and epithelial–mesenchymal transition显示文摘 | Bin Zhang Yan Yang Xiaoting Shi Wanyu Liao Min Chen Alfred Sze-Lok Cheng Hongli Yan Cheng Fang Shu Zhang Guifang Xu Shanshan Shen Shuling Huang Guangxia Chen Ying Lv Tingsheng Ling Xiaoqi Zhang Lei Wang Yuzheng Zhuge Xiaoping Zou | 2014 | Cancer Letters2014，，： | 2 |
| 11 | Interaction between curcumin and mimetic biomembrane显示文摘Curcumin,a major bioactive compound in turmeric,has a broad spectrum of antioxidant,anticarcinogenic,antimutagenic and anti-inflammatory properties.At the molecular level,curcumin modulates many structurally unrelated membrane proteins through several signaling pathways.Curcumin has been suggested to change the properties of cell membranes and affect the membrane-bound proteins indirectly;however,the detailed mechanism has yet to be investigated.In this paper,self-assembled bilayer lipid membranes are artificially constructed on the surface of a gold electrode to mimic biomembranes,and interaction between the supported membranes and curcumin is studied electrochemically.Results show that curcumin interacts with the membranes strongly,in a concentration-dependent manner.At low concentrations,curcumin tends to insert into the outer monolayer only,while at high concentrations,it may also begin to penetrate the inner monolayer.The results obtained in this work may enhance our understanding of the effect of curcumin,and possibly flavonoids,on cell membranes and membrane proteins. | CHEN GuiFang CHEN YangYang YANG NaNa ZHU XueJun SUN LiZhou LI GenXi | 2012 | Science China(Life Sciences)2012，55，6： | 2 |
| 12 | Efficacy of Yinzhihuang Granules and Entecavir on Hepatitis B-induced Cirrhosis显示文摘[Objectives]This study aimed to observe the efficacy and safety of Yinzhihuang granules and entecavir in the treatment of hepatitis B-induced cirrhosis.[Methods]Total 80 patients with decompensated hepatitis B-induced cirrhosis were evenly and randomly divided into two groups,treatment group and control group.The patients in the treatment group were given orally with 0.25 mg entecavir and 5000 mg Yinzhihuang granules once a day,and those in the control group were given orally with 0.5 mg entecavir once a day.The levels of aspartate aminotransferase(AST),alanine aminotransferase(ALT),hyaluronic acid(HA)and HBV DNA,the Child-Pugh score,and the positive situation of HBeAg in different groups were observed before treatment and 12,24,48 and 96 weeks after the treatment,respectively.[Results]The serum ALT,AST,HA and HBV DNA levels,the HBeAg positive rate,and the Child-Pugh score in the treatment and control groups were significantly improved after the treatment(P<0.05).The improvement of the above indicators in the treatment group at week 96 was significantly better than that in the control group(P<0.05).[Conclusions]The combination of Yinzhihuang granules and entecavir is superior to entecavir in the treatment of decompensated hepatitis B-induced cirrhosis. | Fen TANG Guifang TANG Ruiling CHEN Zhenyi LIU Riming WEI | 2019 | Medicinal Plant2019，10，5： | 2 |
| 13 | Control of de novo root regeneration efficiency by developmental status of Arabidopsis leaf explants显示文摘De novo root regeneration(DNRR) has wide applications in agriculture such as those related to cutting technology. Detached Arabidopsis thaliana leaf explants can regenerate adventitious roots without added hormones. The regenerative ability is highly dependent on the developmental status of the leaf. An immature leaf has a higher regenerative ability, while a mature leaf is difficult to regenerate. Using RNASeq analysis, we showed that the expression levels of many genes, including those in the auxin network,changed during leaf maturation. Particularly, the expression levels of many YUCCA(YUC) genes in the auxin biosynthesis pathway are responsive to leaf maturation. Overexpression of YUC1 in the yuc-1 D dominant mutant rescued the rooting defects caused by leaf maturation. In addition, YUC4 expression levels were also affected by circadian rhythms. The regenerative ability was reduced in both immature and mature mutant leaf explants from the new wuschel-related homeobox 11-3(wox11-3) and wox12-3 mutant alleles created by the CRISPR/Cas9 method. Overall, the transcriptome and genetic data, together with the auxin concentration analysis, indicate that the ability to upregulate auxin levels upon detachment may be reduced during leaf maturation. Thus, multiple developmental and environmental signals may converge to control auxin accumulation, which affects the efficiency of the WOX11/12-mediated DNRR from leaf explants. | Jing Pan Fei Zhao Guifang Zhang Yu Pan Lijun Sun Ning Bao Peng Qin Lyuqin Chen Jie Yu Yijing Zhang Lin Xu | 2019 | Journal of Genetics and Genomics2019，46，3： | 2 |
| 14 | CD95-CD95L interaction mediates the growth control of MHV68 immortalized B cells by cytotoxic T cells显示文摘Dear Editor,The main limitation of the viral pathogenesis study of human gammaherpesviruses Epstein-Barr virus(EBV)and Kaposi sarcoma-associated herpesvirus(KSHV)is the absence of animal model owing to the narrow host tropism of both EBV and KSHV.Murine gammaherpesvirus 68(MHV68)encodes several genes involved | Sihan Dong Lingbing Tan Guifang Chen Xiaozhen Liang | 2017 | Virologica Sinica2017，32，3： | 2 |
| 15 | Green fluorescent protein as a vital elimination marker to easily screen marker-free transgenic progeny derived from plants co-transformed with a double T-DNA binary vector system显示文摘 | Songbiao Chen Xugang Li Xiang Liu Hongling Xu Kun Meng Guifang Xiao Xiaoli Wei Feng Wang Zhen Zhu | 2005 | Plant Cell Reports2005，，9： | 1 |
| 16 | Cellular DDX21 RNA helicase inhibits influenza A virus replication but is counteracted by the viral NS1 protein显示文摘 | Guifang Chen Chien-Hung Liu Ligang Zhou Robert M. Krug | 2014 | Cell Host & Microbe2014，，： | 1 |
| 17 | Hot corrosion behaviour of low Al NiCoCrAlY cladded coatings reinforced by nano-particles on a Ni-base super alloy显示文摘 | Hongyu Wang Dunwen Zuo Gang Chen Guifang Sun Xiangfeng Li Xu Cheng | 2010 | Corrosion Science2010，，10： | 1 |
| 18 | The RNA N^(6)-methyladenosine demethylase ALKBH9B modulates ABA responses in Arabidopsis显示文摘The mRNA modification N^(6)-methyladenosine(m^(6)A)plays vital roles in plant development and biotic and abiotic stress responses.The RNA m^(6)A demethylase ALKBH9 B can remove m^(6)A in alfalfa mosaic virus RNA and plays roles in alfalfa mosaic virus infection in Arabidopsis.However,it is unknown whether ALKBH9 B also exhibits demethylation activity and has a biological role in endogenous plant mRNA.We demonstrated here that mRNA m^(6)A modification is induced by the phytohormone abscisic acid(ABA)and that ALKBH9 B has m^(6)A demethylation activity on endogenous mRNA.Knocking out ALKBH9 B led to hypersensitivity to ABA treatment during seed germination and early seedling development.We further showed that ALKBH9 B removes the m^(6)A modification in the ABA INSENSITIVE 1(ABI1)and BRI1-EMS-SUPPRESSOR 1(BES1)transcripts following ABA treatment,affecting the stability of these mRNAs.Furthermore,we determined that ALKBH9 B acts genetically upstream of the transcription factors ABI3 and ABI5,and its regulatory function in ABA responses depended on ABI3 and ABI5.Our findings reveal the important roles of the m^(6)A modification in ABA responses and highlight the role of ALKBH9 Bmediated m^(6)A demethylation in regulating ABA responses post-transcriptionally. | Jun Tang Junbo Yang Qiang Lu Qian Tang Shuyan Chen Guifang Jia | 2022 | Journal of Integrative Plant Biology2022，64，12： | 1 |
| 19 | Sediment rating parameters and their implications:Yangtze River,China显示文摘 | Yang Guifang Chen Zhongyuan Yu Fengling | 2007 | Geomorphology2007，85，34： | 1 |
| 20 | Saikosaponin?d suppresses the expression of cyclooxygenase?2 throughthe phospho?signal transducer and activator of transcription 3/hypoxia?induciblefactor?1α pathway in hepatocellular carcinoma cells显示文摘 | Shuixiang He Guifang Lu Helei Hou Zhenjun Zhao Zhanfang Zhu Xinlan Lu Jinghong Chen Zhilun Wang | 2014 | Molecular Medicine Reports2014，，5： | 1 |
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