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| 1 | A putative 6-transmembrane nitrate transporter OsNRT1.1b plays a key role in rice under low nitrogen显示文摘OsNRT1.1a is a low-affinity nitrate(NO_3^-) transporter gene. In this study, another mRNA splicing product, OsNRT1.1b,putatively encoding a protein with six transmembrane domains, was identified based on the rice genomic database and bioinformatics analysis. OsNRT1.1a/OsNRT1.1b expression in Xenopus oocytes showed OsNRT1.1a-expressing oocytes accumulated ^(15)N levels to about half as compared to OsNRT1.1bexpressing oocytes. The electrophysiological recording of OsNRT1.1b-expressing oocytes treated with 0.25 mM NO_3^- confirmed ^(15)N accumulation data. More functional assays were performed to examine the function of OsNRT1.1b in rice. The expression of both OsNRT1.1a and OsNRT1.1b was abundant in roots and downregulated by nitrogen(N) deficiency. The shoot biomass of transgenic rice plants with OsNRT1.1a or OsNRT1.1b overexpression increased under various N supplies under hydroponic conditions compared to wild-type(WT). The OsNRT1.1a overexpression lines showed increased plant N accumulation compared to the WT in 1.25 mM NH_4NO_3 and 2.5 mM NO_3^- or NH_4^+ treatments, but not in 0.125 mM NH_4NO_3.However, OsNRT1.1b overexpression lines increased total N accumulation in all N treatments, including 0.125 m M NH_4NO_3,suggesting that under low N condition, OsNRT1.1b would accumulate more N in plants and improve rice growth, but also that OsNRT1.1a had no such function in rice plants. | Xiaorong Fan Huimin Feng Yawen Tan Yanling Xu Qisong Miao Guohua Xu | 2016 | Journal of Integrative Plant Biology2016，58，6： | 21 |
| 2 | Bipolar transurethral enucleation and resection of the prostate:Whether it is ready to supersede TURP?显示文摘Objective:According to the EAU Guidelines,transurethral resection of the prostate(TURP)has so far still been considered as the gold standard for surgical treatment for patients with obstructing clinical benign prostate hyperplasia(BPH).However,its relatively high rate of complications and postoperative recurrence necessitates further modification and innovation on the surgery technique.We reported the patient outcomes with our technique.Methods:We retrospectively analyzed 52 patients with obstructing clinical BPH who underwent bipolar transurethral enucleation and resection of the prostate(B-TUERP)between March 2015 and September 2015.Pre-and perioperative parameters were obtained from medical charts.Postoperative follow-ups were administrated at 1,3,6,12 and 24 month(s)after surgery,respectively.Results:All the operations were performed successfully with a mean operative time of 43.1 min and an average tissue removal rate of 74.7%.Qmax was significantly improved immediately after surgery,followed by a continuous improvement throughout the follow-ups.Following a steep decrease in mean prostate specific antigen(PSA)and post void residual(PVR)observed within the first half year after surgery,the serum PSA was then maintained at a constant level of 0.61 ng/mL.Temporary urinary retention was found in four cases(7.7%).Stress urinary incontinence occurred in five patients(9.6%),with the condition resolved in 1e2 weeks without extra treatment.Urethral strictures and bladder neck contractures,as the most commonly observed long-term complications,developed in four patients(7.7%).No recurrence was found during 2 years of follow-ups.An improvement in International Index of Erectile Function(IIEF-5)scores was witnessed in 17 patients preoperatively with normal sexual function during the first 6 months after surgery,and sustained throughout the 24-month period.Conclusions:Enucleation reflects an improvement on surgical technique in many ways with a need for surgical equipment that can be broadly accessible in clinical practice.Currently,bipolar resection is a commonly employed procedure in clinical settings,and its similarity shared with bipolar enucleation technique warrants a quick learning of B-TUERP by urologists.Based on these findings,we believe that the substitution of TURP by TUERP as the gold standard for prostate endoscopic procedure can be expected in the future. | Peng Xu Abai Xu Binshen Chen Shaobo Zheng Yawen Xu Hulin Li Haiyan Shen Chunxiao Liu | 2018 | Asian Journal of Urology2018，5，1： | 19 |
| 3 | The transition from traditional banking to mobile internet finance:an organizational innovation perspective-a comparative study of Citibank and ICBC显示文摘The development of Financial Technology(FinTech)in areas such as mobile Internet,cloud computing,big data,search engines,and blockchain technology have significantly changed the financial industry.FinTech is expected to overturn the traditional banking business model,forcing banks to upgrade and transform.This study adopts a comparative case study method to contrast and analyze the Industrial and Commercial Bank of China(ICBC)and Citibank.It analyzes the strategies,organizations,HR systems,and product innovations adopted by these two banks in response to the impact of FinTech.This paper proposes an“electric vehicle”mode for ICBC and an“airplane mode”for Citibank.Further,it describes the difficulties encountered by the Chinese banking industry and proposes some feasible ways to upgrade.“Technology power”will become the core competitive concept for the financial institutions of the future. | Zhuming Chen Yushan Li Yawen Wu Junjun Luo | 2017 | Financial Innovation2017，3，1： | 19 |
| 4 | Advances and prospects of rare earth metal-organic frameworks in catalytic applications显示文摘In recent years,rare earth metal-organic frameworks(RE-MOFs)have attracted increasing attention because of abundant coordination behaviors,adjustable channels and stable networks.The various electron structures of rare earth ions contribute to the catalytic applications of RE-MOFs and their derivates.In this review,we systematically summarize the research progress that using RE-MOFs and their derivates as catalysts for organic reaction,photocatalytic reaction,and CO oxidation reaction.The major parts include synthetic strategies and catalytic applications of the RE-MOFs.Finally,impressive achievements of RE-MOF catalysts are emerging and a prospect is provided for the development of RE-MOF catalysts at the end of the review. | Xiaochen Sun Kun Yuan Yawen Zhang | 2020 | Journal of Rare Earths2020，38，8： | 16 |
| 5 | Synthesis and electrocatalytic activity of Au@Pd coreshell nanothorns for the oxygen reduction reaction显示文摘有多孔的表面的二金属的核心壳 nanostructures 在各种各样的领域里由于他们的有希望的应用引起了可观的注意,包括催化作用和电子学。在这个工作,有不平、多孔的表面的 Au@Pd 核心壳 nanothorns (CSNT ) 面对 polyallylamine hydrochloride 通过一个灵巧的合作化学药品减小方法第一次被综合(哼) 并且乙烯乙二醇(例如) 在房间温度。Au@Pd CSNT 的尺寸,形态学,和作文被传播电子显微镜学(TEM ) 调查, X 光检查衍射(XRD ) ,精力散光谱学(EDX ) ,印射的 EDX,和 X 光检查光电子光谱学(XPS ) 。同样综合的 Au@Pd CSNT 的电气化学的性质被各种各样的电气化学的技术也学习。Au@Pd CSNT 在碱的媒介为氧减小反应(ORR ) 展出了显著地高的 electrocatalytic 活动和耐久性,由于在 Au 核心和 Pd 壳之间的唯一的多孔的结构和 synergistic 效果。 | Gengtao Fu Zhenyuan Liu Yu Chen Jun Lin Yawen Tang Tianhong Lu | 2014 | Nano Research2014，7，8： | 15 |
| 6 | GBSS-BINDING PROTEIN,encoding a CBM48 domain-containing protein,affects rice quality and yield^∞显示文摘The percentage of amylose in the endosperm of rice(Oryza sativa)largely determines grain cooking and eating qualities.Granule-bound starch synthase I(GBSSI)and GBSSII are responsible for amylose biosynthesis in the endosperm and leaf,respectively.Here,we identified OsGBP,a rice GBSS-binding protein that interacted with GBSSI and GBSSII in vitro and in vivo.The total starch and amylose contents in osgbp mutants were significantly lower than those of wild type in leaves and grains,resulting in reduced grain weight and quality.The carbohydrate-binding module 48(CBM48)domain present in the C-terminus of OsGBP is crucial for OsGBP binding to starch.In the osgbp mutant,the extent of GBSSI and GBSSII binding to starch in the leaf and endospermwas significantly lower than wild type.Our data suggest that OsGBP plays an important role in leaf and endosperm starch biosynthesis by mediating the binding of GBSS proteins to developing starch granules.This elucidation of the function of OsGBP enhances our understanding of the molecular basis of starch biosyn-thesis in rice and contributes information that can be potentially used for the genetic improvement of yield and grain quality. | Wei Wang Xiangjin Wei Guiai Jiao Wenqiang Chen Yawen Wu Zhonghua Sheng Shikai Hu Lihong Xie Jiayu Wang Shaoqing Tang Peisong Hu | 2020 | Journal of Integrative Plant Biology2020，62，7： | 12 |
| 7 | Neddylation of PTEN regulates its nuclear import and promotes tumor development显示文摘PTEN tumor suppressor opposes the PI3K/Akt signaling pathway in the cytoplasm and maintains chromosomal integrity in the nucleus.Nucleus–cytoplasm shuttling of PTEN is regulated by ubiquitylation,SUMOylation and phosphorylation,and nuclear PTEN has been proposed to exhibit tumor-suppressive functions.Here we show that PTEN is conjugated by Nedd8 under high glucose conditions,which induces PTEN nuclear import without effects on PTEN stability.PTEN neddylation is promoted by the XIAP ligase and removed by the NEDP1 deneddylase.We identify Lys197 and Lys402 as major neddylation sites on PTEN.Neddylated PTEN accumulates predominantly in the nucleus and promotes rather than suppresses cell proliferation and metabolism.The nuclear neddylated PTEN dephosphorylates the fatty acid synthase(FASN)protein,inhibits the TRIM21-mediated ubiquitylation and degradation of FASN,and then promotes de novo fatty acid synthesis.In human breast cancer tissues,neddylated PTEN correlates with tumor progression and poor prognosis.Therefore,we demonstrate a previously unidentified pool of nuclear PTEN in the Nedd8-conjugated form and an unexpected tumor-promoting role of neddylated PTEN. | Ping Xie Zhiqiang Peng Yujiao Chen Hongchang Li Mengge Du Yawen Tan Xin Zhang Zhe Lu Chun-Ping Cui Cui Hua Liu Fuchu He Lingqiang Zhang | 2021 | Cell Research2021，31，3： | 11 |
| 8 | Research progress on FASN and MGLL in the regulation of abnormal lipid metabolism and the relationship between tumor invasion and metastasis显示文摘Tumorigenesis involves metabolic reprogramming and abnormal lipid metabolism,which is manifested by increased endogenous fat mobilization,hypertriglyceridemia,and increased fatty acid synthesis.Fatty acid synthase(FASN)is a key enzyme for the de novo synthesis of fatty acids,and monoacylglycerol esterase(MGLL)is an important metabolic enzyme that converts triglycerides into free fatty acids.Both enzymes play an important role in lipid metabolism and are associated with tumor-related signaling pathways,the most common of which is the PI3K–AKT signaling pathway.They can also regulate the immune microenvironment,participate in epithelial–mesenchymal transition,and then regulate tumor invasion and metastasis.Current literature have shown that these two genes are abnormally expressed in many types of tumors and are highly correlated with tumor migration and invasion.This article introduces the structures and functions of FASN and MGLL,their relationship with abnormal lipid metabolism,and the mechanism of the regulation of tumor invasion and metastasis and reviews the research progress of the relationship of FASN and MGLL with tumor invasion and metastasis. | Jingyue Zhang Yawen Song Qianqian Shi Li Fu | 2021 | Frontiers of Medicine2021，15，5： | 10 |
| 9 | Ultrathin AgPt alloy nanowires as a high-performance electrocatalyst for formic acid oxidation显示文摘探讨不够的 electrocatalytic 活动和蚁的酸氧化反应(FAOR ) 的稳定性 electrocatalysts,以及他们的高度花费了,我们此处表明 ultrathin AgPt 合金 nanowires 的灵巧的热水的合成用终止胺 poly (N-isopropylacrylamide )(PNIPAM-NH 2) 作为一个指导结构的代理人。AgCl 的起始的产生猛抛, AgPt nanoparticles 的随后的形成,和他们的面向的附件说明 ultrathin AgPt 合金 nanowires 的形成。得益于他们的唯一的 1D anisotropy 和 alloyed 作文,准备 ultrathin AgPt nanowires 展览为 FAOR 的一项优异 electrocatalytic 活动和更好的公司忍耐,到达 1.6 褶层和 3.7 褶层更高特定的当前的密度比 AgPt nanoparticles 和商业磅黑人催化剂分别地。另外, ultrathin AgPt 合金 nanowires 在 electrocatalysis 期间表明优异电气化学的稳定性和结构的坚韧性,使他们成为有希望的 FAOR electrocatalyst。这个工作不仅为高贵基于金属的 ultrathin nanowires 的合成提供可靠策略,而且在新窗户中为燃料房间 systems.Open 图象向有效 electrocatalysts | Xian Jiang Gengtao Fu Xia Wu Yang Liu Mingyi Zhang Dongmei Sun Lin Xu Yawen Tang | 2018 | Nano Research2018，11，1： | 9 |
| 10 | Nine compounds from the root bark of Lycium chinense and their anti-inflammatory activities显示文摘Two new compounds, named lyciumlignan D(1) and lyciumphenyl propanoid A(2), along with seven known compounds, were isolated from the root bark of Lycium chinense. Their structures were elucidated using spectroscopic data(UV, IR, HR-ESI-MS, 1D and 2D NMR, CD), as well as by comparison with those of the literature. Compounds 3–9 were isolated from this genus for the first time. In the in vitro assay, compounds 3, 6, and 7 exhibited stronger anti-inflammatory effects than the positive control curcumin at a concentration of 10 μmol/L. | Yanan Yang Yawen An Wei Wang Ning Du Jinghua Zhang Ziming Feng Jianshuang Jiang Peicheng Zhang | 2017 | Acta Pharmaceutica Sinica B2017，7，4： | 9 |
| 11 | Spinel MnCo2O4 nanoparticles cross-linked with two-dimensional porous carbon nanosheets as a high-efficiency oxygen reduction electrocatalyst显示文摘为氧减小反应(ORR ) 的催化剂在燃料房间起一个重要作用。有到基于磅的催化剂的可比较的 ORR 活动的其他的非宝贵的金属催化剂为燃料房间的发展是高度合乎需要的。在这个工作,我们第一次报导尖晶石 MnCo 2 O 4/C ORR 催化剂由一致 MnCo 2 O 4 nanoparticles cross-linked 与二维( 2D )多孔的碳 nanosheets (缩短的同样多孔的 MnCo 2 O 4/C nanosheets ),在哪个葡萄糖作为模板被用作碳来源和 NaCl 。获得的多孔的 MnCo 2 在场的 O 4/C nanosheets 互连的多孔的体系结构和一个大表面区域的联合性质(175.3 m 2( | Gengtao Fu Zhenyuan Liu Jingfei Zhang Jiayan Wu Lin Xu Dongmei Sun Jubing Zhang Yawen Tang Pei Chen | 2016 | Nano Research2016，9，7： | 7 |
| 12 | sLe^(x) expression in invasive micropapillary breast carcinoma is associated with poor prognosis and can be combined with MUC1/EMA as a supplementary diagnostic indicator显示文摘Objective: Mucin 1(MUC1/EMA) and sialyl Lewis X(sLe^(x)) indicate polarity reversal in invasive micropapillary carcinoma(IMPC). The purpose of this study was to evaluate the expression of MUC1/EMA and sLe^(x) and to assess their diagnostic and prognostic value in patients with IMPC.Methods: The expression of sLe^(x) and MUC1/EMA in 100 patients with IMPC and a control group of 89 patients with invasive ductal carcinoma not otherwise specified(IDC-NOS) were analyzed with IHC. Fresh tumor tissues were collected from patients with IMPC or IDC-NOS for primary culture and immunofluorescence analysis.Results: The rate of nodal metastasis was higher in patients with IMPC than those with IDC-NOS, and IMPC cells tended to express more sLe^(x) and MUC1/EMA in the cytomembranes(the stroma-facing surfaces of the micropapillary clusters) than IDC-NOS cells. In IMPC, high cytomembrane expression of sLe^(x), but not MUC1/EMA, indicated poor prognosis. In addition, among the 100 patients with IMPC, 10 patients had sLe^(x)+/EMA-expression patterns, and 8 patients had sLe^(x)-/EMA+ expression patterns. The primary IMPC cells were suspended, non-adherent tumor cell clusters, whereas the primary IDC cells were adherent tumor cells. Immunofluorescence analysis showed that MUC1/EMA and sLe^(x) were co-expressed on the cytomembranes in IMPC cell clusters and in the cytoplasm in IDC-NOS cells.Conclusions: sLe^(x) can be used as a prognostic indicator and can be combined with MUC1/EMA as a complementary diagnostic indicator to avoid missed IMPC diagnosis. | Yawen Song Hui Sun Kailiang Wu Jianke Lyu Jingyue Zhang Feng Gu Yongjie Ma Beibei Shen Chijuan Wang Xiaojiao Chen Jing Xu Weidong Li Fangfang Liu Li Fu | 2021 | Cancer Biology & Medicine2021，18，2： | 7 |
| 13 | Facile synthesis based on novel carbon-supported cyanogel of structurally ordered Pd3Fe/C as electrocatalyst for formic acid oxidation显示文摘 | Zhenyuan Liu Gengtao Fu Jiahui Li Zhenqi Liu Lin Xu Dongmei Sun Yawen Tang | 2018 | Nano Research2018，11，9： | 6 |
| 14 | Global pattern and change of cropland soil organic carbon during 1901-2010: Roles of climate, atmospheric chemistry, land use and management显示文摘Soil organic carbon(SOC)in croplands is a key property of soil quality for ensuring food security and agricultural sustainability,and also plays a central role in the global carbon(C)budget.When managed sustainably,soils may play a critical role in mitigating climate change by sequestering C and decreasing greenhouse gas emissions into the atmosphere.However,the magnitude and spatio-temporal patterns of global cropland SOC are far from well constrained due to high land surface heterogeneity,complicated mechanisms,and multiple influencing factors.Here,we use a process-based agroecosystem model(DLEM-Ag)in combination with diverse spatially-explicit gridded environmental data to quantify the long-term trend of SOC storage in global cropland area during 1901-2010 and identify the relative impacts of climate change,elevated CO2,nitrogen deposition,land cover change,and land management practices such as nitrogen fertilizer use and irrigation.Model results show that the total SOC and SOC density in the 2000s increased by 125%and 48.8%,respectively,compared to the early 20th century.This SOC increase was primarily attributed to cropland expansion and nitrogen fertilizer use.Factorial analysis suggests that climate change reduced approximately 3.2%(or 2,166 Tg C)of the total SOC over the past 110 years.Our results indicate that croplands have a large potential to sequester C through implementing better land use management practices,which may partially offset SOC loss caused by climate change. | Wei Ren Kamaljit Banger Bo Tao Jia Yang Yawen Huang Hanqin Tian | 2020 | Geography and Sustainability2020，1，1： | 6 |
| 15 | Dendritic platinum-copper bimetallic nanoassemblies with tunable composition and structure: Arginine-driven self-assembly and enhanced electrocatalytic activity显示文摘 | Gengtao Fu Huimin Liu Nika You Jiayan Wu Dongmei Sun Lin Xu Yawen Tang Yu Chen | 2016 | Nano Research2016，9，3： | 6 |
| 16 | A 38-Year Climatology of Explosive Cyclones over the Northern Hemisphere显示文摘Explosive cyclones(ECs)over two basins in the Northern Hemisphere(20°-90°N)from January 1979 to December2016 are investigated using ERA-Interim and Optimum Interpolation Sea Surface Temperature(OISST)data.The classical definition of an EC is modified considering not only the rapid drop of the central sea level pressure of the cyclone,but also the strong wind speed at the height of 10 m in which maximum wind speeds greater than 17.2 m s^-1are included.According to the locations of the northern Atlantic and northern Pacific,the whole Northern Hemisphere is divided into the'A region'(20°-90°N,90°W-90°E)and'P region'(20°-90°N,90°E-90°W).Over both the A and P regions,the climatological features of ECs,such as their spatial distribution,intensity,seasonal variation,interannual variation,and moving tracks,are documented. | Gang FU Yawen SUN Jilin SUN Pengyuan LI | 2020 | Advances in Atmospheric Sciences2020，37，2： | 6 |
| 17 | Genome-wide DNA methylation profiles in Tibetan and Yorkshire pigs under highaltitude hypoxia显示文摘Background: Tibetan pigs, which inhabit the Tibetan Plateau, exhibit distinct phenotypic and physiological characteristics from those of lowland pigs and have adapted well to the extreme conditions at high altitude.However, the genetic and epigenetic mechanisms of hypoxic adaptation in animals remain unclear.Methods: Whole-genome DNA methylation data were generated for heart tissues of Tibetan pigs grown in the highland(TH, n = 4) and lowland(TL, n = 4), as well as Yorkshire pigs grown in the highland(YH, n = 4) and lowland(YL, n = 4), using methylated DNA immunoprecipitation sequencing.Results: We obtained 480 million reads and detected 280679, 287224, 259066, and 332078 methylation enrichment peaks in TH, YH, TL, and YL, respectively. Pairwise TH vs. YH, TL vs. YL, TH vs. TL, and YH vs. YL comparisons revealed6829, 11997, 2828, and 1286 differentially methylated regions(DMRs), respectively. These DMRs contained 384, 619,192, and 92 differentially methylated genes(DMGs), respectively. DMGs that were enriched in the hypoxia-inducible factor 1 signaling pathway and pathways involved in cancer and hypoxia-related processes were considered to be important candidate genes for high-altitude adaptation in Tibetan pigs.Conclusions: This study elucidates the molecular and epigenetic mechanisms involved in hypoxic adaptation in pigs and may help further understand human hypoxia-related diseases. | Bo Zhang Dongmei Ban Xiao Gou Yawen Zhang Lin Yang Yangzom Chamba Hao Zhang | 2019 | Journal of Animal Science and Biotechnology2019，10，2： | 6 |
| 18 | Flowering responses to light and temperature显示文摘Light and temperature signals are the most important environmental cues regulating plant growth and development. Plants have evolved various strategies to prepare for, and adapt to environmental changes. Plants integrate environmental cues with endogenous signals to regulate various physiological processes, including flowering time. There are at least five distinct pathways controlling flowering in the model plant Arabidopsis thaliana: the photoperiod pathway, the vernalization/thermosensory pathway, the autonomous floral initiation, the gibberellins pathway, and the age pathway. The photoperiod and temperature/vernalization pathways mainly perceive external signals from the environment, while the autonomous and age pathways transmit endogenous cues within plants. In many plant species, floral transition is precisely controlled by light signals(photoperiod) and temperature to optimize seed production in specific environments. The molecular mechanisms by which light and temperature control flowering responses have been revealed using forward and reverse genetic approaches. Here we focus on the recent advances in research on flowering responses to light and temperature. | Li Li Xu Li Yawen Liu Hongtao Liu | 2016 | Science China(Life Sciences)2016，59，4： | 6 |
| 19 | Sensitivity of Potential Evapotranspiration Estimation to the Thornthwaite and Penman–Monteith Methods in the Study of Global Drylands显示文摘Drylands are among those regions most sensitive to climate and environmental changes and human-induced perturbations.The most widely accepted definition of the term dryland is a ratio,called the Surface Wetness Index(SWI),of annual precipitation to potential evapotranspiration(PET)being below 0.65.PET is commonly estimated using the Thornthwaite(PET Th)and Penman–Monteith equations(PET PM).The present study compared spatiotemporal characteristics of global drylands based on the SWI with PET Th and PET PM.Results showed vast differences between PET Th and PET PM;however,the SWI derived from the two kinds of PET showed broadly similar characteristics in the interdecadal variability of global and continental drylands,except in North America,with high correlation coefficients ranging from 0.58 to 0.89.It was found that,during 1901–2014,global hyper-arid and semi-arid regions expanded,arid and dry sub-humid regions contracted,and drylands underwent interdecadal fluctuation.This was because precipitation variations made major contributions,whereas PET changes contributed to a much lesser degree.However,distinct differences in the interdecadal variability of semi-arid and dry sub-humid regions were found.This indicated that the influence of PET changes was comparable to that of precipitation variations in the global dry–wet transition zone.Additionally,the contribution of PET changes to the variations in global and continental drylands gradually enhanced with global warming,and the Thornthwaite method was found to be increasingly less applicable under climate change. | Qing YANG Zhuguo MA Ziyan ZHENG Yawen DUAN | 2017 | Advances in Atmospheric Sciences2017，34，12： | 6 |
| 20 | Au nanoclusters suppress chronic lymphocytic leukaemia cells by inhibiting thioredoxin reductase 1 to induce intracellular oxidative stress and apoptosis显示文摘Chronic lymphocytic leukaemia(CLL) is a rare blood cancer that always relapses as refractory disease and eventually leads to death. To date, therapeutic options for CLL patients are scarce and there is an urgent need to develop novel chemotherapeutics that are both effective and safe. Gold-containing compounds induce a lethal oxidative and endoplasmic reticulum stress response in cultured and primary CLL cells via inhibition of thioredoxin reductase(TrxR). However, traditional gold-containing medicines have revealed side effects during clinical applications. Therefore, safer gold-containing drugs are needed to overcome this challenge. In this study, a novel peptide templated gold cluster Au_(25)Sv_9 was synthesized and its therapeutic effect on CLL cells was evaluated. This nanocluster could induce cell apoptosis in MEC-1 cells in a dose-dependent manner which correlated with the uptake amount of clusters in cells.As expected, increasing intracellular reactive oxidative species(ROS) in MEC-1 cells was exhibited with the increase of cluster dosage. Further analyses demonstrated the underlying mechanism that the nanoclusters suppress the activity of TrxR1, increase the level of intracellular ROS, destroy the mitochondrial membrane potential and finally trigger the mitochondrial apoptotic pathway in MEC-1 cells.Furthermore, the direct interaction between Au_(25)Sv_9 clusters and TrxR1 was confirmed for the first time by isothermal titration calorimetry. These findings explored the preclinical efficacy and potential mechanism of gold clusters in CLL therapy and provided a fundamental reference for the development of other novel gold-containing chemotherapeutics to treat CLL. | Qiong Li Qing Yuan Mohan Zhao Yawen Yao Liang Gao Ru Liu Yaling Wang Yong Gong Fuping Gao Xueyun Cao | 2017 | Science Bulletin2017，62，8： | 5 |
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