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| 1 | Extensive translation of circular RNAs driven by N6-methyladenosine显示文摘广泛的 pre-mRNA 拼接背在人的 transcriptome 产生众多的圆形的 RNA (circRNAs ) 。然而,这些 circRNAs 的生物功能仍然保持大部分不清楚。这里,我们报导那 N 6-methyladenosine (m 6 一) , RNA 的最丰富的基础修正,在人的房间从 circRNAs 支持蛋白质翻译的有效开始。我们发现那一致 m 6 A 主题在 circRNAs 和单个 m 6 一个地点是足够的驾驶翻译开始。这 m 6A 驱动的翻译要求开始因素 eIF4G2 和 m 6 读者 YTHDF3,并且被 methyltransferase METTL3/14 提高,在热吃惊之上由 demethylase FTO,和 upregulated 禁止了。通过介绍的 polysome 的进一步的分析,计算预言和集体 spectrometry 揭示那 m 6 circRNAs 的 A 驱动的翻译是普遍的,与有翻译潜力的几百内长的 circRNAs。我们的学习扩展人的 transcriptome 的编码风景,并且在对环境应力的细胞的回答建议导出 circRNA 的蛋白质的一个角色。 | Yun Yang Xiaojuan Fan Miaowei Mao Xiaowei Song Ping Wu6,7 Yang Zhang Yongfeng Jin Yi Yang Ling-Ling Chen Yang Wang Catherine CL Wong Xinshu Xiao Zefeng Wang | 2017 | Cell Research2017，27，5： | 246 |
| 2 | Prevalence of Autism Spectrum Disorder in China:A Nationwide Multi-center Population-based Study Among Children Aged 6 to 12 Years显示文摘This study aimed to obtain the first national estimate of the prevalence of autism spectrum disorder(ASD) in Chinese children.We targeted the population of 6 to 12-year-old children for this prevalence study by multistage convenient cluster sampling.The Modified Chinese Autism Spectrum Rating Scale was used for the screening process.Of the target population of 142,086 children,88.5%(n=125,806) participated in the study.A total of 363 children were confirmed as having ASD.The observed ASD prevalence rate was 0.29%(95% CI:0.26%-0.32%) for the overall population.After adjustment for response rates,the estimated number of ASD cases was867 in the target population sample,thereby achieving an estimated prevalence of 0.70%(95% CI:0.64%-0.74%).The prevalence was significantly higher in boys than in girls(0.95%;95% CI:0.87%-1.02% versus 0.30%;95%CI:0.26%-0.34%;P <0.001).Of the 363 confirmed ASD cases,43.3% were newly diagnosed,and most of those(90.4%) were attending regular schools,and 68.8% of the children with ASD had at least one neuropsychiatric comorbidity.Our findings provide reliable data on the estimated ASD prevalence and comorbidities in Chinese children. | Hao Zhou Xiu Xu Weili Yan Xiaobing Zou Lijie Wu Xuerong Luo Tingyu Li Yi Huang Hongyan Guan Xiang Chen Meng Mao Kun Xia Lan Zhang Erzhen Li Xiaoling Ge Lili Zhang Chunpei Li Xudong Zhang Yuanfeng Zhou Ding Ding Andy Shih Eric Fombonne Yi Zheng Jisheng Han Zhongsheng Sun Yong-hui Jiang Yi Wang LATENT-NHC Study Team | 2020 | Neuroscience Bulletin2020，36，9： | 158 |
| 3 | RNA-seq analysis of prostate cancer in the Chinese population identifies recurrent gene fusions, cancer-associated long noncoding RNAs and aberrant alternative splicings显示文摘在有前列腺癌症的不同赛跑的病人之中有显著不同;然而,位于这差别下面的机制仍然保持不清楚。这里,我们在场 transcriptome 的一处全面风景用 RNA-seq,越过关于基因熔化的前列腺癌症 transcriptomes 的揭示巨大的差异,长 noncoding RNA (长 ncRNA ) ,其他的拼接和体的变化从中国人口 14 主要前列腺癌症和他们的配对的正常对应物介绍。14 个肿瘤(21.4%) 中的三个在中国病人怀有 TMPRSS2 尔格熔化,和这熔化的低流行进一步在一个另外的肿瘤集合(10/54=18.5%) 被证实。尤其是,二新奇基因熔化, CTAGE5-KHDRBS3 (20/54=37%) 和 USP9Y-TTTY15 (19/54=35.2%) ,在我们的耐心的队经常发生了。介绍的进一步系统的 transcriptional 识别了是在肿瘤表示的差别的众多的长 ncRNAs。在长 ncRNA 和基因的表示之间的关联的分析建议长 ncRNAs 可以在 transcriptional 规定以外有功能。这研究在中国人口产出新卓见进前列腺癌症的致病。 | Shancheng Ren Zhiyu Peng Jian-Hua Mao Yongwei Yu Changjun Yin Xin Gao Zilian Cui Jibin Zhang Kang Yi Weidong Xu Chao Chen Fubo Wang Xinwu Guo Ji Lu Jun Yang Min Wei Zhijian Tian Yinghui Guan Liang Tang Chuanliang Xu Linhui Wang Xu Gao Wei Tian Jian Wang Huanming Yang Jun Wang Yinghao Sun | 2012 | Cell Research2012，22，5： | 66 |
| 4 | Mesenchymal stem cells derived from human placenta suppress allogeneic umbilical cord blood lymphocyte proliferation显示文摘ABSTRACTHuman placenta-derived mononuclear cells (MNC) were isolated by a Percoll density gradient and cultured in mesen-chymal stem cell (MSC) maintenance medium. The homogenous layer of adherent cells exhibited a typical fibroblast-like morphology, a large expansive potential, and cell cycle characteristics including a subset of quiescent cells. In vitrodifferentiation assays showed the tripotential differentiation capacity of these cells toward adipogenic, osteogenic andchondrogenic lineages. Flow cytometry analyses and immunocytochemistry stain showed that placental MSC was ahomogeneous cell population devoid of hematopoietic cells, which uniformly expressed CD29, CD44, CD73, CD105,CD166, laminin, fibronectin and vimentin while being negative for expression of CD31, CD34, CD45 and α-smoothmuscle actin. Most importantly, immuno-phenotypic analyses demonstrated that these cells expressed class I majorhistocompatibility complex (MHC-I), but they did not express MHC-II molecules. Additionally these cells could sup-press umbilical cord blood (UCB) lymphocytes proliferation induced by cellular or nonspecific mitogenic stimuli. Thisstrongly implies that they may have potential application in allograft transplantation. Since placenta and UCB arehomogeneous, the MSC derived from human placenta can be transplanted combined with hematopoietic stem cells(HSC) from UCB to reduce the potential graft -versus-host disease (GVHD) in recipients. | Chang Dong LI Wei Yuan ZHANG He Lian LI Xiao Xia JIANG Yi ZHANG Pei Hsien TANG Ning MAO | 2005 | Cell Research2005，15，7： | 36 |
| 5 | Sufentanil reduces emergence agitation in children receiving sevoflurane anesthesia for adenotonsillectomy compared with fentanyl显示文摘背景出现焦虑是在小儿科的麻醉的一个普通问题,特别在 sevoflurane 正式就职和这研究的维护 anesthesia.The 目的是调查在与 fentanyl.Methods 相比经历 adenotonsillectomy 的孩子在 sevoflurane 麻醉以后减少出现焦虑的 sufentanil 的效果以后 105 个孩子 3-11 年,随机被分配收到正常盐(控制组), sufentanil 0.2 g/kg ( S2 )或芬太奴 2 g/kg ( F2 ) 1 | LI Jun HUANG Zhi-lian ZHANG Xu-tong LUO Ke ZHANG Zhan-qin MAO Yi ZHUANG Xiao-biao LIAN Qing-quan CAO Hong | 2011 | Chinese Medical Journal2011，，22： | 27 |
| 6 | Postoperative adjuvant antiviral therapy for hepatitis B/C virus-related hepatocellular carcinoma:A meta-analysis显示文摘AIM:To investigate the impact of postoperative antiviral treatment on tumor recurrence and survival of patients with chronic hepatitis B virus(HBV) or hepatitis C virus(HCV) infection-related primary hepatocellular carcinoma(HCC) after curative therapy.METHODS:We performed a meta-analysis of randomized and non-randomized control trials from electronic search and manual search.The fixed effect model of Mantel-Haenszel method and the random effect model of Der Simonian and Laird method were used for homogeneous and heterogeneous studies,respectively.Seven HCV-related studies,three HBV-related studies and three studies on HBV or HCV-related HCC were identified.RESULTS:A total of 1224 patients were included in this analysis.The estimated odds ratios(OR) for the 1-,2-,3-and 5-year recurrence were 0.54 [15.4% vs 24.1%,95% confidence interval(CI):0.32-0.89,P=0.02],0.42(36.9% vs 58.0%,95% CI:0.19-0.90,P=0.03),0.37(47.9% vs 63.8%,95% CI:0.19-0.71,P=0.003),and 0.32(66.7% vs 74.3%,95% CI:0.15-0.66,P=0.002),respectively;and the OR for the 1-,2-,3-,5-and 7-year mortality were 0.23(1.2% vs 9.1%,95% CI:0.07-0.71,P=0.01),0.31(6.4% vs 22.1%,95% CI:0.12-0.79,P=0.01),0.43(12.7% vs 20.8%,95% CI:0.21-0.89,P=0.02),0.42(25.1% vs 42.0%,95% CI:0.27-0.66,P=0.0002) and 0.28(31.9% vs 52.2%,95% CI:0.13-0.59,P=0.0008).CONCLUSION:This meta-analysis indicates the postoperative antiviral therapy,interferon in particular,may serve as a favorable alternative to reduce recurrence and mortality in patients with HBV/HCV related HCCs. | Miao, Ruo-Yu Zhao, Hai-Tao Yang, Hua-Yu Mao, Yi-Lei Lu, Xin Zhao, Yi Liu, Chang-Ning Zhong, Shou-Xian Sang, Xin-Ting Huang, Jie-Fu | 2010 | World Journal of Gastroenterology2010，16，23： | 26 |
| 7 | Evaluation of the Effects of Standard Rescue Procedure on Severe Trauma Treatment in China显示文摘 | Xiao-Feng Yin Tian-Bing Wang Pei-Xun Zhang Yu-Hui Kou Dian-Ying Zhang Kai Yu De-Cheng Lyu Mao-Zheng Liu Dong-Sheng Zhou Peng Zhang Jue-Hua Jing Wei-Wei Ge Li-Ying Cao Guo-Sheng Wang Shao-Jie Deng Weng-Hua Liu Mao Zhang Yong-An Xu Kun Zhang Bing Li Wei Wang Zhong-Li Gao Cheng-La Yi Bao-Guo Jiang | 2015 | Chinese Medical Journal2015，，10： | 18 |
| 8 | Recurrent gain-of-function USP8 mutations in Cushing's disease显示文摘库欣的疾病,引起过量皮质醇生产的也已知的同样促肾上腺皮质的荷尔蒙(ACTH )-secreting 垂体腺瘤(舞步) ,占多达 85%corticotrophin 依赖的库欣的症候群盒子。然而,在这疾病的基因改变是不清楚的。这里,我们执行了从 12 秘密 ACTH 舞步导出并且匹配血样品的 DNA 定序的 whole-exome,它在候选人基因揭示了体的变化的三种类型, USP8 (编码 ubiquitin 特定的朊酶 8 ) ,专门在 exon 14 在 12 秘密 ACTH 舞步中的 8 个。我们进一步评估了体的 USP8 变化在另外由定序的 Sanger 的 258 舞步。进一步定序指向在 108 秘密 ACTH 舞步(62.04%) 中的 67 个识别了 USP8 变体的 17 种类型的一个总数。然而,任何一个都没在舞步的另外的类型这些变化被检测(n = 150 ) 。这些变化在 USP8 的 14-3-3 绑定主题以内聚集并且破坏在 USP8 和 14-3-3 蛋白质之间的相互作用,导致保护 EGFR 免受 lysosomal 降级的伤害的一个提高的能力。因此,有 EGFR 表示,提高的 EGFR 蛋白质丰富和 mRNA 表示的更高的发生 POMC 铺平的变异的 USP8 显示的舞步,它编码 ACTH 的先锋。有变异的 USP8 的舞步在尺寸是显著地更小的并且比野类型的舞步有更高的 ACTH 生产。在里面通过手术 resected 主要变异 USP8 的肿瘤房间, USP8 击倒或堵住的 EGFR 有效地稀释 ACTH 分泌物。一起拿,体的 gain-of-function USP8 变化是普通的并且在库欣的疾病贡献 ACTH 生产过剩。USP8 或 EGFR 的抑制为对待变异 USP8 的 corticotrophin 腺瘤是有希望的。我们的学习在库欣的疾病加亮潜在地功能的变异的基因并且提供卓见进这疾病的治疗学。 | Zeng-Yi Ma Zhi-Jian Song Jian-Hua Chen Yong-Fei Wang Shi-Qi Li Liang-Fu Zhou Ying Mao Yi-Ming Li Rong-Gui Hu Zhao-Yun Zhang Hong-Ying Ye Ming Shen Xue-Fei Shou Zhi-Qiang Li Hong Peng Qing-Zhong Wang Dai-Zhan Zhou Xiao-Lan Qin Jue Ji Jie Zheng Hong Chen Yin Wang Dao-Ying Geng Wei-Jun Tang Chao-Wei Fu Zhi-Feng Shi Yi-Chao Zhang Zhao Ye Wen-Qiang He Qi-Lin Zhang Qi-Sheng Tang Rong Xie Jia-Wei Shen Zu-Jia Wen Juan Zhou Tao Wang Shan Huang Hui-Jia Qiu Ni-Dan Qiao Yi Zhang Li Pan Wei-Min Bao Ying-Chao Liu Chuan-Xin Huang Yong-Yong Shi Yao Zhao | 2015 | Cell Research2015，25，3： | 16 |
| 9 | Effect of soothing-liver and nourishing-heart acupuncture on early selective serotonin reuptake inhibitor treatment onset for depressive disorder and related indicators of neuroimmunology:a randomized controlled clinical trial显示文摘OBJECTIVE:To observe the effect of soothing-liver and nourishing-heart acupuncture on selective serotonin reuptake inhibitor(SSRIs) treatment effect onset in patients with depressive disorder and related indicators of neuroimmunology.METHODS:Overall,126 patients with depressive disorder were randomly divided into a medicine and acupuncture-medicine group using a random number table.Patients were treated for 6 consecutive weeks.The two groups were evaluated by the Montgomery-Asberg Depression Rating Scale(MADRS) and Side Effects Rating Scale(SERS) to assess the effect of the soothing-liver and nourishing-heart acupuncture method on early onset of SSRI treatment effect.Changes in serum 5-hydroxytryptamine(5-HT) and inflammatory cytokines before and after treatment were recorded and compared between the medicine group and the acupuncture-medicine group.RESULTS:The acupuncture-medicine group had significantly lower MADRS scores at weeks 1,2,4,and 6 after treatment compared with the medicine group(P < 0.01).The acupuncture group had significantly lower SERS scores at weeks 1,2,4,and 6 after treatment compared with the medicine group(P < 0.01).At 6 weeks after treatment,serum 5-HT in the acupuncture-medicine group was significantly higher compared with the medicine group(P <0.01).lnterleukin-6(IL-6) in the acupuncture-medicine group was significantly lower than that in the medicine group(P < 0.01),whereas there was no significant difference in IL-1(3 between the groups(P > 0.05).Anti-inflammatory cytokines IL-4 and IL-10 were significantly higher in the acupuncture-medicine group compared with the medicine group(P < 0.01,P < 0.05,respectively).CONCLUSION:The soothing-liver and nourishing-heart acupuncture method can effectively accelerate the onset of SSRI effects when treating depressive disorder and can significantly reduce the adverse reactions of SSRIs.Moreover,acupuncture can enhance serum 5-HT and regulate the balance of pro-inflammatory cytokines and anti-inflammatory cytokines. | Liu Yi Feng Hui Mo Yali Gao Jingfang Mao Hongjing Song Mingfen Wang Shengdong Yin Yan Liu Wenjuan | 2015 | Journal of Traditional Chinese Medicine2015，35，5： | 13 |
| 10 | Roux-en-Y gastric bypass for Chinese type 2 diabetes mellitus patients with a BMI,28kg/m^2:a multi-institutional study显示文摘Roux-en-Y gastric bypass surgery(RYGB) has been demonstrated to be successful for treating type-II diabetes2mellitus(T2DM) patients with a body mass index(BMI),30 kg/m,but reports of RYGB for T2 DM patients with22 a BMI,28 kg/mare lacking.T2 DM patients with a BMI,28 kg/mwere prospectively recruited to participate in this study in four hospitals.The endpoint was T2 DM remission(defined by fasting blood glucose(FBG) level,110 mg/d L and hemoglobin(Hb)A1c level,6.0% at 12 months postoperatively).Predictors of remission were investigated by univariate and multivariate analyses.Eighty-six patients were assessed.Eighty-five patients underwent RYGB,with one conversion to open surgery.We compared the values of various variables before and after2 surgery.The mean BMI decreased from 24.68±2.12 to 21.72±2.43 kg/m(P,0.001).Fifty-eight(67.4%) patients were not treated by drugs or insulin after surgery,and 20 patients(23.3%) had complete remission of T2 DM at12 months after surgery with an acceptable number of complications.The mean Hb A1 c level in the remission group was significantly lower than that in the non-remission group.Patients with a higher weight,lower Hb A1 c level,higher C-peptide level,and higher FBG level were more likely to have T2 DM remission in multivariate2 analyses.In conclusion,RYGB was effective and safe for treating T2 DM patients with a BMI,28 kg/m.Complete remission can be predicted by cases having a higher weight,lower Hb A1 c level,higher C-peptide level,and higher FBG level. | Hui Liang Wei Guan Yanling Yang Zhongqi Mao Yijun Mei Huan Liu Yi Miao | 2015 | The Journal of Biomedical Research2015，29，2： | 13 |
| 11 | Chemical profiles and anticancer effects of saponin fractions of different polarity from the leaves of Panax notoginseng显示文摘AIM: To evaluate the chemical profiles and cytotoxic effects among the total saponin fraction(TSF), 25% ethanol fraction(25EF), 50% ethanol fraction(50EF), and 85% ethanol fraction(85EF) prepared by macroporous resin from the leaves of Panax notoginseng. METHOD: The simultaneous determination of thirteen main saponins, as well as the chemical profiles of saponin fractions of different polarity, was made by HPLC-DAD and LC-ESI-MSn analysis. The cytotoxic effects were determined against KP4 cells(human pancreatic cancer), NCI-H727 cells(human lung cancer), HepG2 cells(human hepatocellular cancer), and SGC-7901 cells(human gastric adenocarcinoma). RESULTS: Chemical analysis indicated that 85EF possessed the most abundant cytotoxic protopanaxadiol saponins, including the marker saponins F2, 20(R)-Rg3, 20(S)-Rg3, and Rh2. The MTT assay showed that 85EF also had the strongest cytotoxic effects among the four fractions. 25EF showed no anti-proliferative effects, while 50EF and TSF exhibited weak anti-proliferative activity. CONCLUSION: From the aspect of comprehensive utilization of resources, 85EF, enriched with low polarity PPD group saponins, is a new alternative source of anticancer saponins, and a promising botanical preparation for further anticancer studies. | MAO Qian LI Yi LI Song-Lin YANG Jie ZHANG Ping-Hu WANG Qiang | 2014 | Chinese Journal of Natural Medicines2014，12，1： | 12 |
| 12 | Transduction of the Hedgehog signal through the dimerization of Fused and the nuclear translocation of Cubitus interruptus显示文摘分泌蛋白质的刺猬(Hh ) 家庭为在脊椎动物和无脊椎动物的开发是必要的。作为主要 morphogens 之一在后生动物的开发期间,分级的 Hh 信号通过它的细胞质的尾巴的微分 phosphorylation 是越过由 Smoothened (Smo ) 的血浆膜的 transduced,导致小径激活和目标基因的微分表达式。然而,怎么 Smo transduces 经由建筑群糟糕仍然是的 Costal2 (Cos2 )/Fused (Fu ) 的分级的 Hh 信号理解。这里我们在场对由翻译 Smo phosphorylation 信息到 Fu dimerization 和肘脉 interruptus (Ci ) 的一个 Hh 坡度的房间反应的一个模型原子本地化信息。我们的调查结果建议 Smo 的 phosphorylated C 终点招募 Cos2/Fu 建筑群到通过在 Smo 和 Cos2 之间的相互作用的膜,它进一步导致 Fu dimerization。使聚合成二聚物的 Fu 是 phosphorylated 和 transduces 由 phosphorylating Cos2 和 Fu (苏(fu )) 的 Suppressor 的 Hh 信号。我们进一步证明这个过程支持全身的 Ci (Ci155 ) 和 Cos2 或苏(fu ) 的分离,并且导致 Ci155 的 translocation 进原子核,激活目标基因的表示。 | Yanyan Zhang Feifei Mao Yi Lu Wenqing Wu Lei Zhang Yun Zhao | 2011 | Cell Research2011，21，10： | 12 |
| 13 | Deep-learning-based extraction of the animal migration patterns from weather radar images显示文摘Continental coverage and year-round operation of the weather radar networks provide an unprecedented opportunity for studying large-scale airborne migration.The broad and local-scale airborne information collected by these infrastructures can answer many ecological questions.However,extracting and interpreting the biological information from such massive weather radar data remains an intractable problem.Recently,many big-data problems have been solved using the deep learning technology.In this study,the biological information in the weather radar data is identified using the advanced deep learning method.The proposed method consists of two main parts,i.e.,a rendering and casting procedure and an image segmentation procedure based on a convolutional neural network.The biological data are automatically extracted by rendering and mapping,image segmentation,and result masking.By analyzing the typical radar data from single and multiple stations,we partly reveal the intensity and speed of the migration pattern.We present the first feasibility study of the extraction of local and large-scale biological phenomena from the Chinese weather radar network data. | Kai CUI Cheng HU Rui WANG Yi SUI Huafeng MAO Huayu LI | 2020 | Science China(Information Sciences)2020，63，4： | 11 |
| 14 | Ultrasound features of hepatocellular adenoma and the additional value of contrast-enhanced ultrasound显示文摘BACKGROUND: Hepatocellular adenoma(HCA) is a rare benign tumor of the liver. It is of clinical importance to differentiate HCA from other liver tumors, especially hepatocellular carcinoma(HCC). This study aimed to evaluate the characteristic features of HCA by conventional ultrasound and contrast-enhanced ultrasound(CEUS) findings.METHODS: Twenty-six patients(10 males and 16 females; mean age 36.2±5.0 years) with 26 histopathologically proven HCAs were retrospectively identified. According to the maximum diameter of HCAs, they were divided into three groups: <30 mm, 30-50 mm, and >50 mm. Ultrasound examinations were performed with C5-2 broadband curved transducer of Philips i U22 unit(Philips Bothell, WA, USA). For each lesion, a dose of 2.4 m L Sono Vue®(Bracco Imaging Spa, Milan, Italy) was injected as a quick bolus into the cubital vein. Lesions' echogenicity, color-Doppler flow imaging and contrast enhancement patterns were recorded.RESULTS: Grayscale ultrasound revealed that most of HCAs were hypoechoic(73.1%, 19/26). Spotty calcifications were detected in 26.9%(7/26) of the lesions. Color-Doppler flow imaging detected centripetal bulky color flow in 46.2%(12/26) of the HCAs. CEUS showed that 73.1%(19/26) of the HCAs displayed as rapid, complete and homogenous enhancement, and 53.8%(14/26) showed decreased contrast enhancement in the late phase. There was no significant difference in enhancement patterns among different sizes of HCAs(P>0.05). Centripetal enhancement with subcapsular tortuous arteries was common in larger HCAs.CONCLUSIONS: CEUS combined with grayscale and colorDoppler flow imaging helped to improve preoperative diagnosis of HCAs. The characteristic imaging features of HCAs included: rapid homogeneous enhancement and slow washout pattern on CEUS; heterogeneous echogenicity on grayscale ultrasound; and centripetal enhancement with subcapsular tortuous arteries in large HCAs. | Yi Dong Zheng Zhu Wen-Ping Wang Feng Mao Zheng-Biao Ji | 2016 | Hepatobiliary & Pancreatic Diseases International2016，15，1： | 11 |
| 15 | Casticin induces caspase-mediated apoptosis via activation of mitochondrial pathway and upregulation of DR5 in human lung cancer cells显示文摘Objective:To assess if casticin induces caspase-mediated apoptosis via activation of mitochondrial pathway and upregulation of DR5 in human lung cancer ceils.Methods:Human non-small-cell lung carcinoma cell lines H460,AS49 and H157 were cultured in vitro.The cytotoxic activities were determined using MTT assay.The apoptotic cells death was examined by flow cytometry using PI staining and DMA agarose gel electrophoresis.The activities of caspase-3, -8 and -9 were measured via ELISA.Cellular fractionation was determined by flow cytometry to assess release of cytochrome c and the mitochondrial transmembrane potential.Bcl-2/Bcl-XL/ XIAP/Bid/ DR5 and DR4 proteins were analyzed using western blot.Results:The concentrations required for a 50%decrease in cell growth(IC50) ranged from 1.8 to 3.2 Jt M.Casticin induced rapid apoptosis and triggered a series of effects associated with apoptosis by way of mitochondrial pathway,including the depolarization of the mitochondrial membrane,release of cytochrome c from mitochondria,activation of procaspase-9 and -3,and increase of DNA fragments.Moreover, the pan caspase inhibitor zVAD-FMK and the caspase-3 inhibitor zOEVD-FMK suppressed casticin-induced apoptosis.In addition,casticin induced XIAP and Bcl-XL down-regulation, Bax upregulation and Bid clearage.In H157 cell line,casticin increased expression of DRS at protein levels but not affect the expression of DR4.The prelreatmenl with DR5/Fc chimera protein effectively attenuated casticin-induced apoptosis in H157 cells.No correlation was found between cell sensitivity to casticin and that to p53 status,suggesting that casticin induce a p53- independent apoptosis.Conclusions:Our results demonstrate that casticin induces caspase-mediated apoptosis via activation of mitochondrial pathway and upregulation of DRS in human lung cancer cells. | Yuan Zhou Yi Peng Qi-Qi Mao Xia Li Ming-Wu Chen Ting Su Li Tian Nai-Quan Mao Ling-Zhi Long Mei-Fang Quan Fei Liu Su-Fang Zhou Yong-Xiang Zhao | 2013 | Asian Pacific Journal of Tropical Medicine2013，6，5： | 11 |
| 16 | Adenovirus-mediated overexpression of novel mutated IκBα inhibits nuclear factor κB activation in endothelial cells显示文摘Background Nuclear factor κB (NF-κB) overactivation, requiring phosphorylation and degradation of its inhibitor IκBα, is the basis for chronicity of airway inflammation in asthma. Based on our previous plasmid pShuttle-IκBα, carrying an IκBα gene from human placenta, we optimized a novel IκBα mutant (IκBαM) gene, constructed and characterized its replication-deficient recombinant adenovirus (AdIκBαM), and tested whether AdIκBαM-mediated overexpression of IκBαM could inhibit the NF-κB activation in endothelial cells. Methods IκBαM gene (203-1003 bp) encoding 267 amino acids, acquired by site-directed deleting N-terminal phosphorylation sites of serine 32/36, was subcloned into the pShuttle and pGEM-T vectors for further polymerase chain reaction (PCR), restriction digestion, deoxyribonucleic acid (DNA) sequencing and homology analyses. Subsequent to inserting the expression unit of pShuttle-IκBαM, containing cytomegalovirus (CMV) promoter, IκBαM complementary DNA (cDNA) and polyadenylic acid (PolyA) signals, into the type 5 adenovirus (Ad5) vector, the resultant AdIκBαM was packaged in human embryonic kidney (HEK) 293 cells by cotransfection with lipofectamine. Western blot analysis and electrophoretic mobility shift assay were utilized to detect the AdIκBαM-mediated overexpression of IκBαM in HEK293 cells and its suppressive effect on phorbol 12-myristate 13-acetate (PMA)-induced NF-κB activation in human umbilical vein endothelial (ECV304) cells, respectively. Results The relevant nucleotides and deduced amino acids of 801 bp IκBαM gene were consistent with those of IκBα gene (GenBank accession number: M69043). The titer of the prepared AdIκBαM was 4.0×1012 plaque-forming units (pfu)/L. Moreover, the IκBαM gene was overexpressed in HEK293 cells, and potently inhibited the PMA-induced NF-κB activation in ECV304 cells dose-dependently. Conclusions AdIκBαM is a novel vector for both efficient transfer and specific overexpression of IκBαM gene, as well as potent inhibition of NF-κB activity, providing a promising strategy for gene therapy of asthma. | ZHOU Lin-fu YIN Kai-sheng ZHU Zi-lu ZHU Yi YAO Xin MAO Hui XIE Wei-ping HUANG Mao | 2005 | Chinese Medical Journal2005，，17： | 10 |
| 17 | Relationship between HLA-DR gene polymorphisms and outcomes of hepatitis B viral infections:A meta-analysis显示文摘AIM:To assess the rigorous relationship between human leukocyte antigens(HLA)-DR alleles and outcomes of hepatitis B virus(HBV) infections by means of metaanalysis.METHODS:Medline/PubMed,EMBASE,CNKI and VIP were searched to identify relevant studies.Study quality was evaluated using the Newcastle-Ottawa Scale.Odds ratios(OR) and 95% confidence interval(95% CI) were pooled using Stata 11.0.Subgroup analyses were performed by ethnicity.Heterogeneity and publication bias analyses were performed to validate the credibility.RESULTS:A total of 2609 patients with chronic hepatitis B and 2606 controls spontaneously recovering from prior HBV infection were included.Meta-analysis showed that HLA-DR*04(OR = 0.72,95% CI:0.60-0.85) and DR*13(OR = 0.27,95% CI:0.19-0.37) alleles were significantly associated with HBV clearance while patients carrying HLA-DR*03(OR = 1.47,95% CI:1.16-1.87) or DR*07(OR = 1.59,95% CI:1.24-2.03) alleles had a significantly increased risk of chronic HBV persistence.For the HLA-DR*01 polymorphism,a significantly association with HBV clearance was found in Chinese Han group(OR = 0.48,95% CI:0.26-0.86),but not found in other ethnic groups(P = 0.191).For other polymorphisms,no association with the HBV infection outcome was found.CONCLUSION:HLA-DR*04 and DR*13 alleles may be the protective factors for HBV clearance and HLADR*03,and DR*07 alleles may be the risk factors for HBV persistence. | Ze-Hui Yan Yi Fan Xiao-Hong Wang Qing Mao Guo-Hong Deng Yu-Ming Wang | 2012 | World Journal of Gastroenterology2012，18，24： | 10 |
| 18 | Developing Hybrid cell-edge and cell-node Dissipative Compact Scheme for Complex Geometry Flows显示文摘Developing high resolution finite difference scheme and enabling the use of this scheme on complex geometry are the aims of this study.High resolution has been achieved by Dissipative Compact Schemes(DCS),however,according to the recent research,applications of DCS on complex geometry may have serious problem for that the Geometric Conservation Law(GCL)is not satisfied,and this may cause numerical instability.To cope with this problem,a new scheme named Hybrid cell-edge and cell-node Dissipative Compact Scheme(HDCS)has been formulated.The formulation of the HDCS contains two steps.First,a new central compact scheme is formulated for the purpose of conveniently fulfilling the GCL,and then dissipation is added on the central scheme by high-order dissipative interpolation of cell-edge variables.The solutions of Euler and Navier-Stokes equations show that the HDCS can be applied successfully on complex geometry,while the DCS may suffer numerical instabilities.Moreover,high resolution of the HDCS may be observed in the test of scattering of acoustic waves by multiple cylinders. | DENG XiaoGang JIANG Yi MAO MeiLiang LIU HuaYong TU GuoHua | 2013 | Science China(Technological Sciences)2013，56，10： | 10 |
| 19 | Cystathionine-γ-lyase ameliorates the histone demethylase JMJD3-mediated autoimmune response in rheumatoid arthritis显示文摘Cystathionine-γ-lyase(CSE),an enzyme associated with hydrogen sulfide(H2S)production,is an important endogenous regulator of inflammation.Jumonji domain-containing protein 3(JMJD3)is implicated in the immune response and inflammation.Here,we investigated the potential contribution of JMJD3 to endogenous CSE-mediated inflammation in rheumatoid arthritis(RA).Upregulated CSE and JMJD3 were identified in synovial fibroblasts(SFs)from RA patients as well as in the joints of arthritic mice.Knocking down CSE augmented inflammation in IL-1β-induced SFs by increasing JMJD3 expression.In addition,CSE−/−mice with collagen-induced arthritis(CIA)developed severe joint inflammation and bone erosion.Conversely,overexpressing CSE inhibited JMJD3 expression by the transcription factor Sp-1 and was accompanied by reduced inflammation in IL-1β-treated SFs.Furthermore,JMJD3 silencing or the administration of the JMJD3 inhibitor GSK-J4 significantly decreased the inflammatory response in IL-1β-treated SFs,mainly by controlling the methylation status of H3K27me3 at the promoter of its target genes.GSK-J4 markedly attenuated the severity of arthritis in CIA mice.In conclusion,suppressing JMJD3 expression by the transcription factor Sp-1 is likely responsible for the ability of CSE to negatively modulate the inflammatory response and reduce the progression of RA. | Weijun Wu Ming Qin Wanwan Jia Zheng Huang Zhongzheng Li Di Yang Mengwei Huang Chenxi Xiao Fen Long Jianchun Mao Philip K.Moore Xinhua Liu Yi Zhun Zhu | 2019 | Cellular & Molecular Immunology2019，16，8： | 9 |
| 20 | Effects of mild hypothermia on the ROS and expression of caspase-3 m RNA and LC3 of hippocampus nerve cells in rats after cardiopulmonary resuscitation显示文摘BACKGROUND: Cardiac arrest(CA) is a common and serious event in emergency medicine. Despite recent improvements in resuscitation techniques, the survival rate of patients with CA is unchanged. The present study was undertaken to observe the effect of mild hypothermia(MH) on the reactive oxygen species(ROS) and the effect of neurological function and related mechanisms.METHODS: Sixty-five healthy male Sprague Dawley(SD) adult rats were randomly(random number) divided into 2 groups: blank control group(n=5) and CPR group(n=60). CA was induced by asphyxia. The surviving rats were randomly(random number) divided into two groups: normothermia CPR group(NT) and hypothermia CPR group(HT). Normothermia of 37 °C was maintained in the NT group after return of spontaneous circulation(ROSC), hypothermal intervention of 32 °C was carried out in the HT group for 4 hours immediately after ROSC. Both the NT and HT groups were then randomly divided into 2 subgroups 12 hours and 24 hours after ROSC(NT-12, NT-24, HT-12, HT-24 subgroups). During observation, the neurological defi cit scores(NDSs) was recorded, then the bilateral hippocampi were obtained from rats' head, and monoplast suspension of fresh hippocampus tissue was made immediately to determine the level of intracellular ROS by flow cytometry. Transmission electron microscope was used to observe the ultramicro changes of cellular nucleus and mitochondria. Reverse transcription-polymerase chain reaction(RT-PCR) was used to determine the expression of caspase-3 m RNA, and western-blotting(WB) was used to determine the level of LC3 in frozen hippocampus tissue. Measured data were analyzed with paired sample t test and One-Way ANOVA.RESULTS: Of 60 rats with CA, 44(73%) were successfully resuscitated and 33(55%) survived until the end of the experiment. The NDSs of rats in the NT and HT groups were more signifi cantly reduced than those in the BC group(F=8.107, P<0.05), whereas the NDSs of rats in the HT-12 and HT-24 subgroups were significantly increased in comparison with those NDSs of rats in the NT-12 and NT-24 subgroups, respectively(t=9.692, P<0.001; t=14.374, P<0.001). The ROS in hippocampus nerve cells in the NT and HT groups signifi cantly increased compared to the BC group(F=16.824, P<0.05), whereas the ROS in the HT-12 and HT-24 subgroups significantly reduced compared with that ROS in the NT-12 and NT-24 subgroups, respectively(t=9.836, P<0.001; t=7.499, P<0.001). The expression of caspase-3 m RNA in hippocampus nerve cells in the NT and HT groups were signifi cantly increased compared to the BC group(F=24.527, P<0.05), whereas the expression of caspase-3 m RNA in rats of the HT-12 and HT-24 subgroups was signifi cantly reduced compared to the NT-12 and NT-24 subgroups, respectively(t=6.935, P<0.001; t=4.317, P<0.001). The expression of LC3B-II/I in hippocampus nerve cells of rats in the NT and HT groups signifi cantlyincreased compared to the BC group(F=6.584, P<0.05), whereas the expression of LC3B-II/I in rats of the HT-12 and HT-24 subgroups significantly reduced compared to the NT-12 and NT-24 subgroups, respectively(t=10.836, P<0.001; t=2.653, P=0.02). Ultrastructure damage of nucleus and mitochondria in the NT group was more evident than in the BC group, and eumorphism of nucleus and mitochondria were maintained in rats of the HT group compared with the NT group.CONCLUSION: Mild hypothermia lessened the injury of nerve cells and improved the neurological function of rats that survived from cardiac arrest by reducing the ROS production of nerve cells and inhibiting the expression of caspase-3 m RNA and LC3, leading to cellular apoptosis and massive autophagy in rats that survived from cardiac arrest after CPR. | Jian Lu Yi Shen Hui-yin Qian Li-jun Liu Bao-chun Zhou Yan Xiao Jin-ning Mao Guo-yin An Ming-zhong Rui Tao Wang Chang-lai Zhu | 2014 | World Journal of Emergency Medicine2014，5，4： | 9 |
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