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| 1 | Long Distance Transmission of SARS-CoV-2 from Contaminated Cold Chain Products to Humans——Qingdao City,Shandong Province,China,September 2020显示文摘Summary What is already known about this topic?Though coronavirus disease 2019(COVID-19)has largely been controlled in China,several outbreaks of COVID-19 have occurred from importation of cases or of suspected virus-contaminated products.Though several outbreaks have been traced to severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)isolated on the outer packaging of cold chain products,live virus has not been obtained. | Huilai Ma Zhaoguo Wang Xiang Zhao Jun Han Yong Zhang Hong Wang Cao Chen Ji Wang Fachun Jiang Jie Lei Jingdong Song Shaofeng Jiang Shuangli Zhu Huihui Liu Dongyan Wang Yao Meng Naiying Mao Yanhai Wang Zhen Zhu Zhixiao Chen Bingling Wang Qinqin Song Haijun Du Qun Yuan Dong Xia Zhiqiang Xia Peipei Liu Yuchao Wu Zijian Feng Ruqin Gao George F.Gao Wenbo Xu | 2021 | China CDC weekly2021，3，30： | 13 |
| 2 | A Nosocomial COVID-19 Outbreak Initiated by an Infected Dockworker at Qingdao City Port-Shandong Province, China, October, 2020显示文摘On October 10,2020,3 cases of coronavirus disease 2019(COVID-19)were detected via routine preadmission nucleic acid screening of inpatients and their visitors at Qingdao Central Hospital.The 3 cases were all related to the Qingdao Chest Hospital.An additional 9 cases were discovered and confirmed through tracing and testing contacts of the 3 initial cases.Viral isolates from all 12 cases were found to have high degrees of genetic homology with the viral isolate from a dockworker at Qingdao Port,referred to as Patient A in this report.A joint investigation team was formed to determine the source of infection and the scale of the outbreak,and to prevent further spread of the virus. | Qun Yuan Zengqiang Kou Fachun Jiang Zhongjie Li Lijie Zhang Huihui Liu Xiang Zhao Dianmin Kang Ruqin Gao Jie Lei | 2020 | China CDC weekly2020，2，43： | 12 |
| 3 | Potentiating CD8^(+) T cell antitumor activity by inhibiting PCSK9 to promote LDLR-mediated TCR recycling and signaling显示文摘Metabolic regulation has been proven to play a critical role in T cell antitumor immunity.However,cholesterol metabolism as a key component of this regulation remains largely unexplored.Herein,we found that the low-density lipoprotein receptor(LDLR),which has been previously identified as a transporter for cholesterol,plays a pivotal role in regulating CD8+T cell antitumor activity.Besides the involvement of cholesterol uptake which is mediated by LDLR in T cell priming and clonal expansion,we also found a non-canonical function of LDLR in CD8+T cells:LDLR interacts with the T-cell receptor(TCR)complex and regulates TCR recycling and signaling,thus facilitating the effector function of cytotoxic T-lymphocytes(CTLs).Furthermore,we found that the tumor microenvironment(TME)downregulates CD8+T cell LDLR level and TCR signaling via tumor cell-derived proprotein convertase subtilisin/kexin type 9(PCSK9)which binds to LDLR and prevents the recycling of LDLR and TCR to the plasma membrane thus inhibits the effector function of CTLs.Moreover,genetic deletion or pharmacological inhibition of PCSK9 in tumor cells can enhance the antitumor activity of CD8+T cells by alleviating the suppressive effect on CD8+T cells and consequently inhibit tumor progression.While previously established as a hypercholesterolemia target,this study highlights PCSK9/LDLR as a potential target for cancer immunotherapy as well. | Juanjuan Yuan Ting Cai Xiaojun Zheng Yangzi Ren Jingwen Qi Xiaofei Lu Huihui Chen Huizhen Lin Zijie Chen Mengnan Liu Shangwen He Qijun Chen Siyang Feng Yingjun Wu Zhenhai Zhang Yanqing Ding Wei Yang | 2021 | Protein & Cell2021，12，4： | 12 |
| 4 | Development of Cryogenic Disinfectants Using in-18℃and-40℃Environments—Worldwide,2021显示文摘Introduction:Since the outbreak of coronavirus disease 2019(COVID-19)in Beijing Xinfadi Wholesale Market,a series of COVID-19 outbreaks has indicated the threat of transmission due to outer surfaces of objects under low temperatures.Therefore,2 kinds of chlorine-containing disinfectants for−18℃and−40℃were developed in order to solve the problem of low temperature disinfection.Methods:The properties of two cryogenic disinfectants were evaluated by low-temperature test.Staphylococcus aureus and Escherichia coli were used as two indicator microorganisms to evaluate the effects of the two cryogenic disinfectants in the laboratory and field tests according to Technical Specifications for Disinfection(2002 version)and Test method for bactericidal effect of disinfectants in laboratory(GB/T 38502-2020).Results:Two disinfectants could remain in the liquid state at−18℃or−40℃in 72 hours without crystallization and precipitation,and the average log values of microbial reduction before and after disinfection(the average killing log value)both on Staphylococcus aureus and Escherichia coli were>3 in the laboratory and field tests.Discussion:The results showed that these two disinfectants remained functional at low temperature to disinfect the packaging of imported cold chain food and surrounding packaging to prevent transmission of COVID-19 from material to people. | Huihui Sun Hongyang Duan Wei Zhang Chen Liang Luyao Li Yuan Lyu Yanyan Chen Jin Shen Liubo Zhang | 2021 | China CDC weekly2021，3，13： | 4 |
| 5 | Efficient production of CYTL1 protein using mouse IgGK signal peptide in the CHO cell expression system显示文摘 | Xiaolin Wang Huihui Liu Wanqiong Yuan Yingying Cheng Wenling Han | 2016 | Acta Biochimica et Biophysica Sinica2016，48，4： | 3 |
| 6 | Development and validation of a CT-based radiomics nomogram for preoperative prediction of tumor histologic grade in gastric adenocarcinoma显示文摘Objectives:To develop and validate a radiomics nomogram for preoperative prediction of tumor histologic grade in gastric adenocarcinoma(GA).Methods:This retrospective study enrolled 592 patients with clinicopathologically confirmed GA(low-grade:n=154;high-grade:n=438)from January 2008 to March 2018 who were divided into training(n=450)and validation(n=142)sets according to the time of computed tomography(CT)examination.Radiomic features were extracted from the portal venous phase CT images.The Mann-Whitney U test and the least absolute shrinkage and selection operator(LASSO)regression model were used for feature selection,data dimension reduction and radiomics signature construction.Multivariable logistic regression analysis was applied to develop the prediction model.The radiomics signature and independent clinicopathologic risk factors were incorporated and presented as a radiomics nomogram.The performance of the nomogram was assessed with respect to its calibration and discrimination.Results:A radiomics signature containing 12 selected features was significantly associated with the histologic grade of GA(P<0.001 for both training and validation sets).A nomogram including the radiomics signature and tumor location as predictors was developed.The model showed both good calibration and good discrimination,in which C-index in the training set,0.752[95%confidence interval(95%CI):0.701-0.803];C-index in the validation set,0.793(95%CI:0.711-0.874).Conclusions:This study developed a radiomics nomogram that incorporates tumor location and radiomics signatures,which can be useful in facilitating preoperative individualized prediction of histologic grade of GA. | Jia Huang Huasheng Yao Yexing Li Mengyi Dong Chu Han Lan He Xiaomei Huang Ting Xia Zongjian Yi Huihui Wang Yuan Zhang Jian He Changhong Liang Zaiyi Liu | 2021 | Chinese Journal of Cancer Research2021，33，1： | 3 |
| 7 | Effects of dietary cottonseed meal protein hydrolysate on growth,antioxidants and immunity of Chinese mitten crab Eriocheir Sinensis显示文摘One hundred and sixty crabs(average initial weight:51.32±0.08 g)were fed with four experimental diets containing cottonseed meal protein hydrolysate(CPH)at 0%(CPH0,control),0.3%(CPH0.3),0.6%(CPH0.6),and 1.2%CPH(CPH1.2).The experiment results show that no difference was observed in specific growth rate and survival rate of crabs fed with CPH diet.Moisture content of crabs fed with CPH0.6 diet was significantly reduced than that of the CPH0 group.Superoxide dismutase and catalase activities of crabs fed with CPH0.6 diet were significantly increased and the difference was not significant between the CPH0.3 and CPH0.6 groups.Malondialdehyde content of CPH0.3 group was significantly lower than that of the CPH0 group.Lysozyme,alkaline phosphatase,and acid phosphatase activities of CPH0.3 diet crabs were significantly higher than that of the CPH0 group.Glutamic-pyruvic transaminase activity of crabs fed with CPH0.3 diet was significantly decreased compared to the CPH0 group.The relative expression levels of Toll1,Toll2,MyD88,LITAF,and ILF-2 of crabs fed with CPH0.3 diet were significantly higher than that of the CPH0 group.The expression level of SOCS2 showed an opposite pattern.After CPH perfusion,the expression levels of SOCS2 and Toll1 in intestine at time 3 h and SOCS2 in hepatopancreas at time 18 h increased significantly to the highest value.The expression level of Toll2,MyD88,LITAF,decreased at times 6 h,6 h,12 h,respectively,then increased gradually.Therefore,supplementation of dietary CPH could improve antioxidant capacity and immune function;the appropriate supplement dosage of CPH for crab could be 0.3%-0.6%.Furthermore,the short-term CPH stimulation could significantly increase or decline the expression levels of immune-related genes at different times after CPH perfusion. | CHENG Huihui LIU Wenbin YUAN Xiangyang JIA Erteng ZHANG Dingdong JIANG Guangzhen | 2020 | Journal of Oceanology and Limnology2020，38，3： | 2 |
| 8 | Genome Size of Alexandrium catenella and Gracilariopsis lemaneiformis Estimated by Flow Cytometry显示文摘Flow cytometry(FCM) technique has been widely applied to estimating the genome size of various higher plants. However, there is few report about its application in algae. In this study, an optimized procedure of FCM was exploited to estimate the genome size of two eukaryotic algae. For analyzing Alexandrium catenella, an important red tide species, the whole cell instead of isolated nucleus was studied, and chicken erythrocytes were used as an internal reference. The genome size of A. catenella was estimated to be 56.48 ± 4.14 Gb(1C), approximately nineteen times larger than that of human genome. For analyzing Gracilariopsis lemaneiformis, an important economical red alga, the purified nucleus was employed, and Arabidopsis thaliana and Chondrus crispus were used as internal references, respectively. The genome size of Gp. lemaneiformis was 97.35 ± 2.58 Mb(1C) and 112.73 ± 14.00 Mb(1C), respectively, depending on the different internal references. The results of this research will promote the related studies on the genomics and evolution of these two species. | DU Qingwei SUI Zhenghong CHANG Lianpeng WEI Huihui LIU Yuan MI Ping SHANG Erlei Zeeshan Niaz QUE Zhou | 2016 | Journal of Ocean University of China2016，15，4： | 2 |
| 9 | Antitumor and immunomodulatory activities of a polysaccharide from Artemisia argyi显示文摘 | Xiaoli Bao Chengzhong Wang Jinjin Liu Huihui Yuan Hongli Zhao Minbo Lan | 2013 | Carbohydrate Polymers2013，，： | 1 |
| 10 | Chitosan-graft-β-cyclodextrin nanoparticles as a carrier for controlled drug release显示文摘 | Zeting Yuan Yajing Ye Feng Gao Huihui Yuan Minbo Lan Kaiyan Lou Wei Wang | 2013 | International Journal of Pharmaceutics (-)2013，，1： | 1 |
| 11 | Photooxidation of Methacrolein in Fe(III)-Oxalate Aqueous System and Its Atmospheric Implication显示文摘Iron and oxalic acids are widely distributed in the atmosphere and easily form ferric oxalate complex(Fe(III)-Ox).The tropospheric aqueous-phase could provide a medium to enable the photo-Fenton reaction with Fe(III)-Ox under solar irradiation.Although the photolysis mechanisms of Fe(III)-Ox have been investigated extensively,information about the oxidation of volatile organic compounds(VOC),specifically the potential for Secondary Organic Aerosol(SOA)formation in the Fe(III)-Ox system,is lacking.In this study,a ubiquitous VOC methacrolein(MACR)is chosen as a model VOC,and the oxidation of MACR with Fe(III)-Ox is investigated under typical atmospheric water conditions.The effects of oxalate concentration,Fe(III)concentration,MACR concentration,and pH on the oxidation of MACR are studied in detail.Results show that the oxidation rate of MACR greatly accelerates in the presence of oxalate when compared with only Fe(III).The oxidation rate of MACR also accelerates with increasing concentration of oxalate.The effect of Fe(III)is found to be more complicated.The oxidation rate of MACR first increases and then decreases with increasing Fe(III)concentration.The oxidation rate of MACR increases monotonically with decreasing pH in the common atmospheric water pH range or with decreasing MACR concentration.The production of ferrous and hydrogen peroxide,pH,and aqueous absorbance are monitored throughout the reaction process.The quenching experiments verify that·OH and O_(2)^(+)are both responsible for the oxidation of MACR.MACR is found to rapidly oxidize into small organic acids with higher boiling points and oligomers with higher molecular weight,which contributes to the yield of SOA.These results suggest that Fe(III)-Ox plays an important role in atmospheric oxidation. | Yu WANG Jie ZHAO Huihui LIU Yuan LI Wenbo DONG Yanlin WU | 2021 | Advances in Atmospheric Sciences2021，38，7： | 1 |
| 12 | The development of simultaneous partial nitrification, ANAMMOX and denitrification (SNAD) process in a single reactor for nitrogen removal显示文摘 | Huihui Chen Sitong Liu Fenglin Yang Yuan Xue Tao Wang | 2008 | Bioresource Technology2008，，4： | 1 |
| 13 | Highly efficient gene editing and single cell analysis of hematopoietic stem/progenitor cells from X-linked sideroblastic anemia patients显示文摘Dear Editor,X-linked sideroblastic anemia(XLSA),which is the most common genetic form of congenital sideroblastic anemia,is typically characterized by reduced heme synthesis and the presence of bone marrow(BM)ring sideroblasts containing pathologic iron deposits in the mitochondria. | Riguo Fang Jingliao Zhang Huihui Yang Jia Shi Huimin Zeng Xiaofan Zhu Dong Wei Pengfei Yuan Tao Cheng Yingchi Zhang | 2021 | Signal Transduction and Targeted Therapy2021，6，8： | 1 |
| 14 | Continuous culture of urine-derived bladder cancer cells for precision medicine显示文摘Dear Editor,Bladder cancer is the most common type of genitourinary cancer in China,with an estimated 80,500 new cases and 32,900 related deaths in 2015 alone(Chen et al.,2016).Unlike many other cancers,there has been no significant improvement in survival rates for bladder cancer over the last three decades.Specific treatment regimens for bladder cancer and their efficacy vary depending not only on clinical stages,but also on associated risk factors and other personal clinical characteristics.Patients with non-muscle invasive bladder cancer(NMIBC)have a high 5-year recurrence rate of 60%-70%(Berdik,2017)and those with muscle invasive bladder cancer(MIBC)has a relatively poor prognosis with approximately 65%risk of death within 5-year follow-up(Kamat et al.,2016).Therefore,there is an urgent need to develop models for bladder cancer to screen for rational treatment strategies by personalized medicine to improve the clinical assessment and treatment of bladder cancer. | Shuai Jiang Jiaqi Wang Chen Yang Renke Tan Jun Hou Yuan Shi Huihui Zhang Shiyu Ma Jianan Wang Mengmeng Zhang George Philips Zengxia Li Jian Ma Wanjun Yu Guohua Wang Yuanming Wu Richard Schlegel Huina Wang Shanbo Cao Jianming Guo Xuefeng Liu Yongjun Dang | 2019 | Protein & Cell2019，10，12： | 1 |
| 15 | Genetic Analysis and Improvement Strategies of Protein Content in Rice Seeds显示文摘Enhancing the nutritional quality of food crops is a way to improve human nutrition and health.Grain protein content(GPC)is an important nutritional quality trait for rice and many other crops.Therefore,it will be very significant to increase GPC and improve rice quality.This paper mainly reviewed the genetic studies GPC in rice,mapping and cloning of QTLs and genes associated with GPC.However,little is known about the molecular mechanism and gene function related to GPC in rice.Thus,here we put forward the genetic strategy by gene pyramiding or molecular marker assisted selection to improve GPC and nutritional quality in rice.This review could provide important information for genetic improvement of grain nutritional quality of rice. | Bo PENG Yanfang SUN Dongyan KONG Ruihua PANG Xiaohua SONG Huilong LI Lin LIU Xian-wen LI Jintao LI Qiying ZHOU Bin DUAN Huihui LIU Yuhan ZUO Yanlin HAO Shizhi SONG Hongyu YUAN | 2017 | Agricultural Biotechnology2017，6，4： | 1 |
| 16 | Arabidopsis DXO1 possesses deNADding and exonuclease activities and its mutation affects defense-related and photosynthetic gene expression^FA显示文摘RNA capping and decapping tightly coordinate with transcription,translation,and RNA decay to regulate gene expression.Proteins in the DXO/Rai1 family have been implicated in mRNA decapping and decay,and mammalian DXO was recently found to also function as a decapping enzyme for NAD+-capped RNAs(NAD-RNA).The Arabidopsis genome contains a single gene encoding a DXO/Rai1 protein,AtDXO1.Here we show that AtDXO1 possesses both NAD-RNA decapping activity and 5?-3?exonuclease activity but does not hydrolyze the m7G cap.The atdxo1 mutation increased the stability of NAD-RNAs and led to pleiotropic phenotypes,including severe growth retardation,pale color,and multiple devel-opmental defects.Transcriptome profiling analysis showed that the atdxo1 mutation resulted in upregulation@of defense-related genes but downregulation of photo-synthesis-related genes.The autoimmunity phenotype of the mutant could be suppressed by either eds1 or npr1 mutation.However,the various phenotypes associated with the atdxo1 mutant could be complemented by an enzymatically inactive AtDXO1.The atdxo1 mutation ap-parently enhances post-transcriptional gene silencing by elevating levels of siRNAs.Our study indicates that AtDXO1 regulates gene expression in various biological and physiological processes through its pleiotropic mo-lecular functions in mediating RNA processing and decay. | Shuying Pan Kai-en Li Wei Huang Huan Zhong Huihui Wu Yuan Wang He Zhang Zongwei Cai Hongwei Guo Xuemei Chen Yiji Xia | 2020 | Journal of Integrative Plant Biology2020，62，7： | 1 |
| 17 | A novel poly (DL-lactic aeid) nanopartiele of nitroxide derivative, 4-ferro eenecarboxyl-2, 2, 6, 6-tetramethyl piperidine-1-oxyl 显示文摘 | Wu Ying Miao Lede Yuan Huihui | 2006 | Chemistry Letters2006，35，7： | 1 |
| 18 | Identification of microRNAs regulating grain filling of rice inferior spikelets in response to moderate soil drying post-anthesis显示文摘The grain filling of inferior spikelets is much less complete than that of superior spikelets in rice cultivars with large panicles and numerous spikelets and is promoted by moderate soil drying(MD)post-anthesis.A growing body of evidence has shown that microRNAs function in regulating grain development.However,little is known about the mechanism of microRNA control of grain filling of inferior spikelets in response to MD.In this study,grain filling of inferior spikelets was promoted by MD treatment in Nipponbare.Small-RNA profiling at the most active grain-filling stage was conducted in inferior spikelets under control(CK)and MD treatment.Of 521 known and 128 novel miRNAs,38 known and 9 novel miRNAs were differentially expressed between the CK and MD treatments.Target genes of differentially expressed miRNAs were involved in multiple developmental and signaling pathways associated with catalytic activity,carbohydrate metabolism,and other functions.Both miR1861 and miR397 were upregulated by MD,leading to a decrease in OsSBDCP1 and OsLAC,two negative regulators of SSIIIa activity and BR signaling,respectively.In contrast,miR1432 abundance was reduced by MD,resulting in upregulation of OsACOT and thus an elevated content of both ABA and IAA.These results suggest that both starch synthesis and phytohormone biosynthesis are regulated by differentially expressed miRNAs in inferior spikelets in response to MD treatment.Our results suggest the molecular mechanisms by which miRNAs regulate grain filling in inferior spikelets of rice under moderate soil drying,providing potential application in agriculture to increase rice yields by genetic approaches. | Zhenning Teng Yinke Chen Youqing Yuan Yaqiong Peng Yake Yi Huihui Yu Zhenxie Yi Jianchang Yang Yan Peng Meijuan Duan Jianhua Zhang Nenghui Ye | 2022 | The Crop Journal2022，10，4： | 1 |
| 19 | Absorption and EPR spectra of some porphyrins and metalloporphyrins显示文摘 | Lan Minbo Zhao Hongli Yuan Huihui | 2007 | Dyes and Pigments2007，74，2： | 1 |
| 20 | Application of anaerobic ammonium-oxidizing consortium to achieve completely autotrophie ammonium and sulfate removal 显示文摘 | Liu Sitong Yang Fenglin Gong Zheng Meng Fangang Chen Huihui Xue Yuan Kenji Furukawa | 2008 | Bioresource Technology2008，99，10： | 1 |
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