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| 1 | The antitumor effect of tanshinone IIA on antiproliferation and decreasing VEGF/VEGFR2 expression on the human non-small cell lung cancer A549 cell line显示文摘The effects of tanshinone IIA on the proliferation of the human non-small cell lung cancer cell line A549 and its possible mechanism on the VEGF/VEGFR signal pathway were investigated.The exploration of the interaction between tanshinone IIA and its target proteins provides a feasible platform for studying the anticancer mechanism of active components of herbs.The CCK-8 assay was used to evaluate the proliferative activity of A549 cells treated with tanshinone IIA(2.5 80 μmol/L) for 24,48 and 72 h,respectively.Flow cytometry was used for the detection of cell apoptosis and cell cycle perturbation.VEGF and VEGFR2 expression were studied by Western blotting.The binding mode of tanshinone IIA within thecrystal structure of the VEGFR2 protein was evaluated with molecular docking analysis by use of the CDOCKER algorithm in Discovery Studio 2.1.The CCK-8 results showed that tanshinone IIA can significantly inhibit A549 cell proliferation in a dose-and time-dependent manner.Flow cytometry results showed that the apoptosis rate of tested group was higher than the vehicle control,and tanshinone IIAtreated cells accumulated at the S phase,which was higher than the vehicle control.Furthermore,the expression of VEGF and VEGFR2 was decreased in Western blot.Finally,molecular docking analysis revealed that tanshinone IIA could be stably docked into the kinase domain of VEGFR2 protein with its unique modes to form H-bonds with Cys917 and π–π stacking interactions with Val848.In conclusion,tanshinone IIA may suppress A549 proliferation,induce apoptosis and cell cycle arrest at the S phase.This drug may suppress angiogenesis by targeting the protein kinase domains of VEGF/VEGFR2. | Jun Xie Jiahui Liu Heng Liu Shihui Liang Meigui Lin Yueyu Gu Taoli Liu Dongmei Wang Hui Gee Sui-lin Mo | 2015 | Acta Pharmaceutica Sinica B2015，5，6： | 26 |
| 2 | Single and competitive adsorption affinity of heavy metals toward peanut shell-derived biochar and its mechanisms in aqueous systems显示文摘Converting peanut shells into biochar by pyrolysis was considered an environmentally friendly and efficient method for agricultural solid waste disposal.The properties of peanut shell-derived biochar(PBC)under different temperature and its adsorption capacity of heavy metals were investigated.It was found that PBC400 exhibited the highest cumulative capability for heavy metals elimination in single solute because of its high specific surface area and rich functional groups.Furthermore,the competitive adsorption revealed that PBC had a substantial difference in adsorption affinity from diverse heavy metal ions,sorption capacity decreased as Pb2+>Cu2+>Cd2+>Ni2+>Zn2+,which was lower than in a single solute.The adsorption process using selected biochar was optimized with respect to p H,reaction time,adsorbent dose,and initial concentration of heavy metals.The kinetic data was well fitted with PSO model,and the Langmuir model was adopted for adsorption equilibrium data in both cases of single solutes and mixed solutes for all heavy metals,which indicated that the removal course was primarily explained by monolayer adsorption,and chemical adsorption occupied an important role.Therefore,peanut shells derived biochar could be a potential and green adsorbent for wastewater treatment. | Rui Shan Yueyue Shi Jing Gu YazhuoWang Haoran Yuan | 2020 | Chinese Journal of Chemical Engineering2020，28，5： | 14 |
| 3 | γ-secretase inhibitor DAPT prevents neuronal death and memory impairment in sepsis associated encephalopathy in septic rats显示文摘 | Huang Man Liu chunhui Hu Yueyu Wang Pengfei Ding Meiping | 2014 | Chinese Medical Journal2014，，5： | 12 |
| 4 | OsBRXL4 Regulates Shoot Gravitropism and Rice Tiller Angle through Affecting LAZY1 Nuclear Localization显示文摘Rice tiller angle is a key agronomic trait that contributes to ideal plant architecture and grain production.LAZY1 (LA1) was previously shown to control tiller angle via affecting shoot gravitropism,but the underlying molecular mechanism remains largely unknown.In this study,we identified an LA1-interacting protein named Brevis Radix Like 4 (OsBRXL4).We showed that the interaction between OsBRXL4 and LA1 occurs at the plasma membrane and that their interaction determines nuclear localization of LA1.We found that nuclear localization of LA1 is essential for its function,which is different from AtLA1,its Arabidopsis ortho.log.Overexpression of OsBRXL4 leads to a prostrate growth phenotype,whereas OsBRXLs RNAi plants,in which the expression levels of OsBRXLI,OsBRXL4,and OsBRXL5 were decreased,display a compact phenotype.Further genetic analysis also supported that OsBRXL4 controls rice tiller angle by affecting nuclear localization of LA1.Consistently,we demonstrated that OsBRXL4 regulates the shoot gravitropism through affecting polar auxin transport as did LA1.Taken together,our study not only identifies OsBRXL4 as a regulatory component of rice tiller angle but also provides new insights into genetic regulation of rice plant architecture. | Zhen Li Yan Liang Yundong Yuan Lei Wang Xiangbing Meng Guosheng Xiong Jie Zhou Yueyue Cai Ningpei Han Lekai Hua Guifu Liu Jiayang Li Yonghong Wang | 2019 | Molecular Plant2019，12，8： | 12 |
| 5 | Graphitic carbon nitride (g-C3N4)-based nanosized heteroarrays: Promising materials for photoelectrochemical water splitting显示文摘Photoelectrochemical(PEC)water splitting is recognized as a sustainable strategy for hydrogen generation due to its abundant hydrogen source,utilization of inexhaustible solar energy,high-purity product,and environment-friendly process.To actualize a practical PEC water splitting,it is paramount to develop efficient,stable,safe,and low-cost photoelectrode materials.Recently,graphitic carbon nitride(g-C3N4)has aroused a great interest in the new generation photoelectrode materials because of its unique features,such as suitable band structure for water splitting,a certain range of visible light absorption,nontoxicity,and good stability.Some inherent defects of g-C3N4,however,seriously impair further improvement on PEC performance,including low electronic conductivity,high recombination rate of photogenerated charges,and limited visible light absorption at long wavelength range.Construction of g-C3N4-based nanosized heteroarrays as photoelectrodes has been regarded as a promising strategy to circumvent these inherent limitations and achieve the high-performance PEC water splitting due to the accelerated exciton separation and the reduced combination of photogenerated electrons/holes.Herein,we summarize in detail the latest progress of g-C3N4-based nanosized heteroarrays in PEC water-splitting photoelectrodes.Firstly,the unique advantages of this type of photoelectrodes,including the highly ordered nanoarray architectures and the heterojunctions,are highlighted.Then,different g-C3N4-based nanosized heteroarrays are comprehensively discussed,in terms of their fabrication methods,PEC capacities,and mechanisms,etc.To conclude,the key challenges and possible solutions for future development on g-C3N4-based nanosized heteroarray photoelectrodes are discussed. | Liqun Wang Wenping Si Yueyu Tong Feng Hou Daniele Pergolesi Jungang Hou Thomas Lippert Shi Xue Dou Ji Liang | 2020 | Carbon Energy2020，2，2： | 10 |
| 6 | Towards active plasmonic response devices显示文摘在科学,化学,物理,和生物学,以及在纳米合并电子学和 photonics 的要求为使小型化的集成电路放大的材料给学科交差的挑战, plasmonics 由处于 nanoscale 电子学的速度和兆兆赫电介质 photonics 的尺寸打破限制用作一座桥。在实时在 plasmonic 性质上启用活跃控制的活跃 plasmonic 系统开创了很多潜在的应用程序。这评论集中于活跃 plasmonic 反应设备的发展。重要进展在对活跃包围媒介的绝缘的性质的控制被完成了,包括液体晶体,聚合物,对光反应变色分子和无机的材料,它接着允许附近的金属 nanostructures 的可逆电浆子回声开关调节。 | Yinghui Sun Lin Jiang Liubiao Zhong Yueyue Jiang Xiaodong Chen | 2015 | Nano Research2015，8，2： | 8 |
| 7 | Labile Organic Matter Content and Distribution as Affected by Six-year Soil Amendments to Eroded Chinese Mollisols显示文摘Labile organic carbon(LOC)is a fraction of soil organic carbon(SOC)with rapid turnover time and is affected by soil fertilization.This investigation characterized the SOC content,LOC content and LOC distribution in the treatment plots of surface soil erosion at five levels(0-,5-,10-,20-and 30-cm erosion).The soil had received contrasting fertilizer treatments(i.e.,chemical fertilizer or chemical fertilizer+manure)for 6 years.This study demonstrated that both SOC and various LOC fractions contents were higher in the plots with fertilizer+manure than in those with fertilizer alone under the same erosion conditions.The SOC and LOC contents decreased as the erosion depth increased.Light fraction organic carbon,particulate organic carbon,easily oxidizable organic carbon(KMnO4-oxydizable organic carbon),and microbial biomass carbon were 27%–57%,37%–47%,20%–25%,and 29%–33%higher respectively in the fertilizer+manure plots,than in the fertilizer alone plots.Positive correlations(p<0.05)between SOC content and different fractions contents were observed in all plots except the correlation between total SOC content and water-soluble organic carbon content in the different fertilization treatments.Obviously,fertilizer+manure treatments would be conducive to the accumulation of LOC and SOC in the Black soil of Northeast China. | SUI Yueyu JIAO Xiaoguang CHEN Wenting LIU Xiaobing ZHANG Xingyi DING Guangwei | 2013 | Chinese Geographical Science2013，23，6： | 7 |
| 8 | Symptoms of long-term exposure to moxa smoke in acupuncturists: a correlation analysis显示文摘OBJECTIVE: To investigate the effects of long-term exposure to moxa smoke on acupuncturists and to consider the association between physical symptoms and gender, age, secondhand smoke(SHS) exposure, and the duration of exposure.METHODS: A self-report, web-based questionnaire was used to evaluate the effects of moxa smoke onacupuncturists. Logistic regression was used to analyze the relationship between physical symptoms and possible correlative factors.RESULTS: A total of 858 questionnaires were analyzed. The data showed that 42.42% of acupuncturists had been exposed to moxa smoke for 5-10 years. The most frequent symptom was tearing(32.98%) and the least frequent symptom was asthma(5.24%). Logistic regression analysis showed that female acupuncturists were slightly more susceptible to cough and tearing than males(cough:OR: 1.583, 95% CI: 1.079, 2.321, P = 0.019; tearing:OR: 1.519, 95% CI: 1.094, 2.108, P = 0.013). Acupuncturists aged over 25 years reported a slightly lower incidence of eye itchiness than those aged under25 years(26-40 years: OR: 0.604, 95% CI: 0.379,0.965, P = 0.035; over 40 years: OR: 0.330, 95% CI:0.114, 0.958, P = 0.042). Ophthalmodynia occurred less in acupuncturists aged 26-40 years(OR: 0.591,95% CI: 0.359, 0.970, P = 0.038) than in those younger than 25 years. The only association between prevalence of symptoms and tobacco use and SHS exposure was that smokers had a lower occurrence of phlegm in the throat(OR: 0.579, 95% CI: 0.392,0.856, P = 0.006). Shortness of breath was less frequent in participants exposed to moxa smoke for more than 5 years(5-10 years: OR: 0.400, 95% CI:0.204, 0.785, P = 0.008; 11-20 years: OR: 0.392, 95%CI: 0.178, 0.864, P = 0.02), but a slightly higher incidence of eye itchiness was found in those with over20 years of moxa exposure(OR: 4.200, 95% CI:1.344, 3.128, P = 0.014).CONCLUSION: The most frequent symptom of moxa smoke exposure in acupuncturists was tear-ing. The association of symptoms with age and duration of exposure to moxa smoke were rather complicated; hence, care should be taken in drawing conclusions about the safety of moxa smoke based solely on its potentially harmful ingredients. | Wang Xiang Yu Chang Tang Yong Chang Shyang Yang Hui Zhang He Jiaozi Zhang Yueyue Jiao Yang Zhuang Zhiqi Yin Haiyan Zhang Chengshun Lu Peng Wu Qiaofeng Yu Shuguang | 2018 | Journal of Traditional Chinese Medicine2018，38，1： | 7 |
| 9 | Effect of Yupingfeng granules on the skin barrier in atopic dermatitis mice models显示文摘OBJECTIVE: To investigate effect of Yupingfeng granules, prepared with Chinese Medicines, on the wound healing and on the expression of aquaporin3(AQP3) and the skin barrier in the animal models of atopic dermatitis(AD).METHODS: Acute skin lesions of AD models were prepared using 2,4-dinitrochlorobenzo(DNCB) in mice and animals were treated with either Yupingfeng granules or placebo for two weeks. Skin wound healing outcome was assessed by measuring skin thickness, weight(quality) of the skin, and trans-epidermal water loss(TEWL). Expression of AQP3 mRNA and protein was assessed by reverse transcriotion polymerase chain reaction(RT-PCR)and immunoblotting, respectively.RESULTS: Yupingfeng granule treatment resulted in significant acceleration of wound healing with63.64% efficiency, which was significantly higher than that of placebo granule treatment(31.82%,P < 0.01 by Wilcoxon Rank-sum test). Skin thickness, weight of the wounded skin, and TEWL were significantly higher in the AD models compared to that of normal animals. Treatment with Yupingfeng granules resulted in significant decrease in skin thickness [(937 ± 31) vs(360 ± 21) μm, P < 0.01],weight of the wounded skin [(42 ± 4) vs(24 ± 5)mg, P < 0.01], and TEWL [(30 ± 4) vs(13 ± 4) g·h^(-1)·m-2, P < 0.01]. Yupingfeng granules also significantly down-regulated mRNA and protein expression of AQP3 in the animal models.CONCLUSION: Our findings suggested that Yupingfeng granules could be used in AD treatment. | Sun Zhanxue Wang Jingjun Kong Yuhong Li Nan Jiang Xiaoyuan Cao Ting Jia Yuanning Zhang Yueyue Zhang Yisheng Cheng Jing | 2018 | Journal of Traditional Chinese Medicine2018，38，6： | 6 |
| 10 | Differential Collision Attack on Reduced FOX Block Cipher显示文摘 | Chen Jie Hu Yupu Zhang Yueyu Dong Xiaoli | 2012 | China Communications2012，9，7： | 5 |
| 11 | Plasmon enhancement for Vernier coupled single-mode lasing from ZnO/Pt hybrid microcavities显示文摘开发单个模式操作并且改进放射激光以便保证 microlasers 和 nanolasers 的实际适用性的表演是必要的。在这份报纸,有 ZnO microcomb 的改变的 nanoscaled 空气差距的二六角形的 microteeth 被用来构造联合耳语画廊洞。这被做完成没有要求任何复杂或复杂的操作完成与 nanoscale 精确放,基于游标效果放射激光的一个稳定的单个模式。光获得和相应紫外放射激光表演通过结合磅 nanoparticles 的局部性的表面电浆子极大地被改进。ZnO/Pt 混合 microcavities 完成了单个模式与更高的方面模式抑制比率和更低的阀值放射激光的紧张的七倍的改进。导致了这改进的机制详细被描述了。 | Yueyue Wang Feifei Qin Junfeng Lu Jitao Li Zhu Zhu Qiuxiang Zhu Ye Zhu Zengliang Shi Chunxiang Xu | 2017 | Nano Research2017，10，10： | 5 |
| 12 | The boreal spring stratospheric final warming and its interannual and interdecadal variability显示文摘Based on the daily NCEP/DOE reanalysis II data,dates of the boreal spring Stratospheric Final Warming(SFW) events during 1979–2010 are defined as the time when the zonal-mean zonal wind at the central latitudes(65°–75°N) of the westerly polar jet drops below zero and never recovers until the subsequent autumn.It is found that the SFW events occur successively from the mid to the lower stratosphere and averagely from the mid to late April with a temporal lag of about 13 days from 10 to 50 hPa.Over the past 32 years,the earliest SFW occurs in mid March whereas the latest SFW happens in late May,showing a clear interannual variability of the time of SFW.Accompanying the SFW onset,the stratospheric circulation transits from a winter dynamical regime to a summertime state,and the maximum negative tendency of zonal wind and the strongest convergence of planetary-wave are observed.Composite results show that the early/late SFW events in boreal spring correspond to a quicker/slower transition of the stratospheric circulation,with the zonal-mean zonal wind reducing about 20/5 m s-1 at 30 hPa within 10 days around the onset date.Meanwhile,the planetary wave activities are relatively strong/weak associating with an out-of-/in-phase circumpolar circulation anomaly before and after the SFW events in the stratosphere.All these results indicate that,the earlier breakdown of the stratospheric polar vortex(SPV),as for the winter stratospheric sudden warming(SSW) events is driven mainly by wave forcing;and in contrast,the later breakdown of the SPV exhibits more characteristics of its seasonal evolution.Nevertheless,after the breakdown of SPV,the polar temperature anomalies always exhibit an out-of-phase relationship between the stratosphere and the troposphere for both the early and late SFW events,which implies an intimate stratosphere–troposphere dynamical coupling in spring.In addition,there exists a remarkable interdecadal change of the onset time of SFW in the mid 1990s.On average,the SFW onset time before the mid 1990s is 11 days earlier than that afterwards,corresponding to the increased/decreased planetary wave activities in late winter-early spring before/after the 1990s. | HU JingGao REN RongCai YU YueYue XU HaiMing | 2014 | Science China Earth Sciences2014，57，4： | 4 |
| 13 | Plasmon-enhanced ZnO whispering-gallery mode lasing显示文摘 | Chunxiang Xu Feifei Qin Qiuxiang Zhu Junfeng Lu Yueyue Wang Jitao Li Yi Lin Qiannan Cui Zengliang Shi Arumugam Gowri Manohari | 2018 | Nano Research2018，11，6： | 4 |
| 14 | New prevalence estimate of Torque Teno virus(TTV) infection in healthy population and patients with chronic viral hepatitis in Jiujiang, China显示文摘Dear Editor,Torque Teno virus(TTV)is a nonenveloped human DNA virus that was isolated from the serum of a patient with transfusion-transmitted hepatitis with unknown etiology in 1997(Nishizawa et al.,1997).TTV is the first human virus with a single-stranded circular DNA genome to be identified,and is recently classified as the Alphatorquevirus genus of the Anelloviridae family by the International Committee on Taxonomy of Viruses(ICTV)(King et al.,2011).TTV shows very high | Jin Peng Yueyue Fang Xuesen Zhao Yihong Peng | 2015 | Virologica Sinica2015，30，3： | 4 |
| 15 | Carrier-free highly drug-loaded biomimetic nanosuspensions encapsulated by cancer cell membrane based on homology and active targeting for the treatment of glioma显示文摘Nanosuspensions,as a new drug delivery system for insoluble drugs,are only composed of a drug and a small amount of stabilizer,which is dispersed in an aqueous solution with high drug-loading,small particle size,high dispersion,and large specific surface area.It can significantly improve the dissolution,bioavailability,and efficacy of insoluble drugs.In this study,paclitaxel nanosuspensions((PTX)NS)were prepared by an ultrasonic precipitation method,with the characteristics of simple preparation and easy repetition.With the help of a homologous targeting mechanism,a kind of glioma C6 cancer cell membrane(CCM)-coated(PTX)NS was developed and modified with DWSW peptide to obtain DWSW-CCM-(PTX)NS with the functions of BBB penetration and tumor targeting.The results showed that the cancer cell membrane could effectively camouflage the nanosuspensions so that it was not cleared by the immune system and could cross the blood-brain-barrier(BBB)and selectively target tumor tissues.Cell uptake experiments and in vivo imaging confirmed that the uptake of DWSW-CCM-(PTX)NS by tumor cells and the distribution in intracranial gliomas increased.Cytotoxicity test and in vivo anti-glioma studies showed that DWSW-CCM-(PTX)NS could significantly inhibit the growth of glioma cells and significantly prolong the survival time of glioma-bearing mice.Finally,the cancer cell membrane coating endowed the nanosuspensions with the biological properties of homologous adhesion and immune escape.This study provides an integrated solution for improving the targeting of nanosuspensions and demonstrates the encouraging potential of biomimetic nanosuspensions applicable to tumor therapy. | Yueyue Fan Yuexin Cui Wenyan Hao Mengyu Chen Qianqian Liu Yuli Wang Meiyan Yang Zhiping Li Wei Gong Shiyong Song Yang Yang Chunsheng Gao | 2021 | Bioactive Materials2021，6，12： | 3 |
| 16 | Evaluating the Brewer–Dobson circulation and its responses to ENSO,QBO,and the solar cycle in different reanalyses显示文摘This study compares the climatology and long-term trend of northern winter stratospheric residual mean meridional circulation(RMMC), as well as its responses to El Ni?o-Southern Oscillation(ENSO), stratospheric Quasi Biennial Oscillation(QBO), and solar cycle in ten reanalyses and a stratosphere-resolving model, CESM1-WACCM. The RMMC is a large-scale meridional circulation cell in the stratosphere, usually referred to as the estimate of the Brewer Dobson circulation(BDC). The distribution of the BDC is generally consistent among multiple reanalyses except that the NOAA twentieth century reanalysis(20RC) largely underestimates it. Most reanalyses(except ERA40 and ERA-Interim) show a strengthening trend for the BDC during 1979–2010. All reanalyses and CESM1-WACCM consistently reveal that the deep branch of the BDC is significantly enhanced in El Ni?o winters as more waves from the troposphere dissipate in the stratospheric polar vortex region. A secondary circulation cell is coupled to the temperature anomalies below the QBO easterly center at 50 hPa with tropical upwelling/cooling and midlatitude downwelling/warming, and similar secondary circulation cells also appear between 50–10 hPa and above 10 hPa to balance the temperature anomalies. The direct BDC response to QBO in the upper stratosphere creates a barrier near 30°N to prevent waves from propagating to midlatitudes, contributing to the weakening of the polar vortex. The shallow branch of the BDC in the lower stratosphere is intensified during solar minima, and the downwelling warms the Arctic lower stratosphere. The stratospheric responses to QBO and solar cycle in most reanalyses are generally consistent except in the two 20 CRs. | Jian Rao YueYue Yu Dong Guo ChunHua Shi Dan Chen DingZhu Hu | 2019 | Earth and Planetary Physics2019，3，2： | 3 |
| 17 | Sub-seasonal to Seasonal Hindcasts of Stratospheric Sudden Warming by BCC_CSM1.1(m):A Comparison with ECMWF显示文摘This study focuses on model predictive skill with respect to stratospheric sudden warming(SSW) events by comparing the hindcast results of BCC_CSM1.1(m) with those of the ECMWF's model under the sub-seasonal to seasonal prediction project of the World Weather Research Program and World Climate Research Program. When the hindcasts are initiated less than two weeks before SSW onset, BCC_CSM and ECMWF show comparable predictive skill in terms of the temporal evolution of the stratospheric circumpolar westerlies and polar temperature up to 30 days after SSW onset. However, with earlier hindcast initialization, the predictive skill of BCC_CSM gradually decreases, and the reproduced maximum circulation anomalies in the hindcasts initiated four weeks before SSW onset replicate only 10% of the circulation anomaly intensities in observations. The earliest successful prediction of the breakdown of the stratospheric polar vortex accompanying SSW onset for BCC_CSM(ECMWF) is the hindcast initiated two(three) weeks earlier. The predictive skills of both models during SSW winters are always higher than that during non-SSW winters, in relation to the successfully captured tropospheric precursors and the associated upward propagation of planetary waves by the model initializations. To narrow the gap in SSW predictive skill between BCC_CSM and ECMWF, ensemble forecasts and error corrections are performed with BCC_CSM. The SSW predictive skill in the ensemble hindcasts and the error corrections are improved compared with the previous control forecasts. | Jian RAO Rongcai REN Haishan CHEN Xiangwen LIU Yueyue YU Yang YANG | 2019 | Advances in Atmospheric Sciences2019，36，5： | 3 |
| 18 | Hyperthermia based individual in situ recombinant vaccine enhances lymph nodes drainage for de novo antitumor immunity显示文摘The continuing challenges that limit effectiveness of tumor therapeutic vaccines were high heterogeneity of tumor immunogenicity, low bioactivity of antigens, as well as insufficient lymph nodes(LNs) drainage of antigens and adjuvants. Transportation of in situ neoantigens and adjuvants to LNs may be an effective approach to solve the abovementioned problems. Therefore, an FA-TSL/AuNCs/SV nanoplatform was constructed by integrating simvastatin(SV) adjuvant loaded Au nanocages(AuNCs)as cores(AuNCs/SV) and folic acid modified thermal-sensitive liposomes(FA-TSL) as shells to enhance de novo antitumor immunity. After accumulation in tumor guided by FA, AuNCs mediated photothermal therapy(PTT) induced the release of tumor-derived protein antigens(TDPAs) and the shedding of FATSL. Exposed AuNCs/SV soon captured TDPAs to form in situ recombinant vaccine(AuNCs/SV/TDPAs). Subsequently, AuNCs/SV/TDPAs could efficiently transport to draining LNs owing to the hyperthermia induced vasodilation effect and small particle size, achieving co-delivery of antigens and adjuvant for initiation of specific T cell response. In melanoma bearing mice, FA-TSL/AuNCs/SV and laser irradiation effectively ablated primary tumor, against metastatic tumors and induced immunological memory. This approach served a hyperthermia enhanced platform drainage to enable robust personalized cancer vaccination. | Cuixia Zheng Xinxin Liu Yueyue Kong Lei Zhang Qingling Song Hongjuan Zhao Lu Han Jiannan Jiao Qianhua Feng Lei Wang | 2022 | Acta Pharmaceutica Sinica B2022，12，8： | 2 |
| 19 | Leigongteng(Radix et Rhizoma Tripterygii) via compatibility with Jinqiancao(Herba Lysimachiae): its toxicity-reduced efficacy in H22-bearing mice显示文摘OBJECTIVE: To observe toxicity-reduced effects of Leigongteng(Radix et Rhizoma Tripterygii)(LGT) via compatibility with Jinqiancao(Herba Lysimachiae)(JQC) in H22-bearing mice and investigate the possible underlying mechanism, and further explore whether JQC can enhance LGT-evoked anti-tumor effect.METHODS: H22-bearing mice were orally administered with LGT alone and its compatibility with JQC, and tumors, serum, livers and kidneys were collected to evaluate the toxicity-reduced efficacy and the possible mechanism.RESULTS: LGT evoked significantly elevated biochemical indicators including serum alanine/aspartate transaminase(ALT/AST), creatinine(Cr) and urea nitrogen(BUN) as well as pathological damage in mice, which were all obviously reversed by JQC via compatibility at the ratios from 4/1 to 1/4.Further analysis indicated that pro-inflammatory cytokine tumor necrosis factor-alpha(TNF-α), and malondialdehyde(MDA) levels significantly decreased, while anti-inflammatory cytokine interleukin(IL)-10, and glutathione(GSH), GSH-s transferase(GST), GSH peroxidase(GSH-Px), superoxide dismutase(SOD) and catalase(CAT) levels all increased in livers and kidneys of mice. Besides, after compatibility with JQC at the ratios of 4/1, 2/1, 1/1,1/2 and 1/4, LGT-decreased tumor weight was further decreased by 48.4%, 57.3%, 54.0%, 49.3% and52.9%, respectively(all P < 0.01).CONCLUSION: JQC could reduce LGT-induced hepatotoxicity and nephrotoxicity, and enhance the antitumor efficacy via compatibility with JQC, and the toxicity-reduced mechanism could involve inhibiting hepatic and kidney oxidative stress and inflammation. | Wang Junming Li Jinhua Cai Hong Zhang Yueyue Li Jinyang Cui Ying | 2019 | Journal of Traditional Chinese Medicine2019，39，4： | 2 |
| 20 | Effects of miR-101-3p on goat granulosa cells in vitro and ovarian development in vivo via STC1显示文摘Background:MiRNAs act as pivotal post-transcriptional gene mediators in the regulation of diverse biological processes,including proliferation,development and apoptosis.Our previous study has showed that miR-101-3p is differentially expressed in dairy goat ovaries compared single with multiple litters.The objective of this research was to explore the potential function and molecular mechanism of miR-101-3p via its target STC1 in goat ovarian growth and development.Results:cDNA libraries were constructed using goat granulosa cells transfected with miR-101-3p mimics and negative control by RNA-sequencing.In total,142 differentially expressed unigenes(DEGs)were detected between two libraries,including 78 down-regulated and 64 up-regulated genes.GO and KEGG enrichment analysis showed the potential impacts of DEGs on ovarian development.STC1 was singled out from DEGs for further research owing to it regulates reproductive-related processes.In vitro,bioinformatics analysis and 3′-UTR assays confirmed that STC1 was a target of miR-101-3p.ELISA was performed to detect the estrogen(E2)and progesterone(P4)levels.CCK8,EdU and flow cytometry assays were performed to detect the proliferation and apoptosis of granulosa cells.Results showed that miR-101-3p regulated STAR,CYP19A1,CYP11A1 and 3β-HSD steroid hormone synthesis-associated genes by STC1 depletion,thus promoted E2 and P4 secretions.MiR-101-3p also affected the key protein PI3K,PTEN,AKT and mTOR in PI3K-AKT pathway by STC1,thereby suppressing proliferation and promoting apoptosis of granulosa cells.In vivo,the distribution and expression levels of miR-101-3p in mouse ovaries were determined through fluorescence in situ hybridisation(FISH).Immunohistochemistry results showed that STC1 expression was suppressed in mouse ovaries in miR-101-3p-agonist and siRNA-STC1 groups.Small and stunted ovarian fragments,decreased numbers of follicles at diverse stages were observed using Hematoxylin-eosin(HE)staining,thereby showing unusual ovarian development after miR-101-3p overexpression or STC1 depletion.Inhibition of miR-101-3p manifested opposite results.Conclusions:Taken together,our results demonstrated a regulatory mechanism of miR-101-3p via STC1 in goat granulosa cells,and offered the first in vivo example of miR-101-3p and STC1 functions required for ovarian development. | Xiaopeng An Haidong Ma Yuhan Liu Fu Li Yuxuan Song Guang Li Yueyu Bai Binyun Cao | 2021 | Journal of Animal Science and Biotechnology2021，12，2： | 2 |
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