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| 1 | Acupuncture treatment of lung-spleen Qi deficiency in stable chronic obstructive pulmonary disease: a randomized, open-label, controlled trial显示文摘OBJECTIVE:To evaluate the effectiveness and safety of acupuncture that reinforces the spleen to strengthen the lung in patients with stable chronic obstructive pulmonary disease(COPD).METHODS:This was a randomized,open-controlled trial in which the acupuncturist and the participants were not blinded,but the outcome evalu-ators and data analysts were blinded.One-hundred-and-two patients with stable COPD were randomly divided into two groups in a 1∶1 ratio.The acupuncture group received 30-minute sessions of acupuncture therapy at the same acupoints three times weekly for 6 weeks in addition to routine conventional Western Medicine treatment;the control group received routine conventional Western Medicine treatment alone.The primary outcome was the Borg scale score,which was assessed immediately after the 6-minute walk test.The secondary outcomes were the 6-minute walk distance,lung function,and oxygen saturation.Measurements were obtained at baseline and after 6 weeks of treatment.RESULTS:After 6 weeks of treatment,the Borg scale score in the acupuncture group was significantly better than that in the control group(2.02±0.71 versus 5.01±0.34,P<0.05).Furthermore,the post-treatment improvements in the 6-minute walk distance,lung function,and oxygen saturation were significantly greater in the acupuncture group than in the control group,showing that the acupuncture group had better exercise tolerance.CONCLUSION:The findings suggest that acupuncture that aims to reinforce the spleen to strengthen the lung is a safe and effective adjuvant therapy that effectively improves the exercise capacity of patients with stable COPD. | Li Yu Xiong Chan Zeng Yan Wei Hua Zhuang Guangtong Zhao Lihong Li Chenyi Li Lin Qin Erqi Chen Xiaoqin Fu Juanjuan | 2019 | Journal of Traditional Chinese Medicine2019，39，6： | 13 |
| 2 | Rapid degradation of hexachlorobenzene by micron Ag/Fe bimetal particles显示文摘The feasibility of the rapid degradation of hexachlorobenzene (HCB) by micron-size silver (Ag)/iron (Fe) particles was investigated.Ag/Fe particles with different ratios (0,0.05%,0.09%,0.20%,and 0.45%) were prepared by electroless silver plating on 300 mesh Fe powder,and were used to degrade HCB at different pH values and temperatures.The dechlorination ability of Fe greatly increased with small Ag addition,whereas too much added Ag would cover the Fe surface and reduce the effective reaction surface,thereby decreasing the extent of dechlorination.The optimal Ag/Fe ratio was 0.09%.Tafel polarization curves showed that HCB was rapidly degraded at neutral or acidic pH,whereas low pH levels severely intensified H2 production,which consumed the reducing electrons needed for the HCB degradation.HCB degradation was more sensitive to temperature than pH.The rate constant of HCB dechlorination was 0.452 min-1 at 85℃,50 times higher than that at 31℃.HCB was degraded in a successive dechlorination pathway,yielding the main products 1,2,4,5-tetrachlorobenzene and 1,2,4-trichlorobenzene within 2 hr. | Xiaoqin Nie Jianguo Liu Xianwei Zeng Dongbei Yue | 2013 | Journal of Environmental Sciences2013，25，3： | 9 |
| 3 | Aerosolized STAT1 Antisense Oligodeoxynucleotides Decrease the Concentrations of Inflammatory Mediators in Bronchoalveolar Lavage Fluid in Bleomycin-Induced Rat Pulmonary Fibrosis显示文摘It has been demonstrated that alveolar macrophages (AMs) play a key role in the pathogenesis of pulmonary fibrosis by releasing a variety of cytokines and inflammatory mediators. In addition, abnormal signal transducer and activator of transcription-1 (STAT1) activation in AMs may play a pivotal role in the process of alveolitis and pulmonary fibrosis. In this study, we transfected STAT1 antisense oligodeoxynucleotide (ASON) into rats by aerosolization, and then investigated the effect of STAT1 ASON on inflammatory mediators such as TGF-β, PDGF and TNF-α in bronchoalveolar lavage fluid (BALF) from rats with bleomycin (BLM)-induced rat pulmonary fibrosis. Our results showed that STAT1 ASON by aerosolization could enter into lung tissues and AMs. STAT1 ASON could inhibit mRNA and protein expressions of STAT1 and ICAM-1 in AMs of rat with pulmonary fibrosis, and had no toxic side effect on liver and kidney. Aerosolized STAT1 ASON could ameliorate the alveolitis through inhibiting the secretion of inflammatory mediators in BLM-induced rat pulmonary fibrosis. These results suggest that aerosolized STAT1 ASON might be considered as a promising new strategy in the treatment of pulmonary fibrosis. | Ming Zeng Bin Liao Chen Zhu Wenjun Wang Xiaoqin Zhan Xianming Fan | 2008 | Cellular & Molecular Immunology2008，5，3： | 8 |
| 4 | Strain Softening and Hardening Behavior in AZ61 Magnesium Alloy显示文摘The deformation behavior of AZ61 Mg alloy during hot deformation has been investigated in wide temperature and strain rate range by a Gleeble simulator. Specimens are deformed in compression in the temperature range of 523~673 K and at strain rates of 0.001~1 s-1. It is found that the flow curves exhibit a peak and then decrease towards steady-state of classical DRX, which decrease with rising temperature and decreasing strain rate. The deformation behavior of the specimens can be attributed to the occurrence of strain hardening and softening. As stress decreases, the strain hardening rate declines at a fast rate when temperature rises or strain rate decreases. The shapes of θ-σ curves indicate some important features such as subgrain formation, the critical stress, the peak stress and steady stress. The onset of DRX can be determined by the point of inflection on θ-σ or Inθ-σ curves. | Haitao ZHOU, Liufa LIU, Qudong WANG, Da LU, Xiaoqin ZENG and Wenjiang DINGNational Engineering Research Center of Light Alloys Net Forming, School of Materials Science and Engineering, Shanghai Jiao Tong University, Shanghai 200030, China | 2004 | Journal of Materials Science & Technology2004，20，6： | 7 |
| 5 | Preparation and hydrogen storage properties of MgH2-trimesic acid-TM MOF(TM=Co,Fe) composites显示文摘Two kinds of metal-organic frameworks(MOFs) based on Co(Ⅱ) and Fe(Ⅱ) as metal ions and trimasic acid(TMA) as organic linker were synthesized. They were used to prepare corresponding Mg H2-TM MOF(TM = Co, Fe) composites via ball-milling. X-ray diffraction analyses show the formation of Mg2 Co and α-Fe phases in Mg H2-TMMOF composites after decomposition. Both of the well dispersed Mg2Co andα-Fe nanoparticles exhibit considerable catalytic efficiency in accelerating the sorption kinetics of Mg H2.The dehydrogenated MgH2-Fe MOF composite shows faster hydriding kinetics than the pure Mg H2 and Mg H2-CoMOF. Meanwhile, the apparent dehydrogenation activation energy(Ed) of the Mg H2-Co MOF and Mg H2-Fe MOF composites are 151.3 ± 9.4 and 142.3 ± 6.5 kJ/mol H2, both of which are lower than that of pure Mg H2(181.4 ± 9.2 kJ/mol H2). The improvement on the sorption kinetics of the Mg H2-TM MOF powders is mainly attributed to the catalytic effects of nano-sized Mg2 Co and α-Fe formed on the surface of Mg/Mg H2 particles. | Zhewen Ma Jianxin Zou Darvaish Khan Wen Zhu Chuanzhu Hu Xiaoqin Zeng Wenjiang Ding | 2019 | Journal of Materials Science & Technology2019，35，10： | 6 |
| 6 | Solute-homogenization model and its experimental verification in Mg-Gd-based alloys显示文摘Composition homogenization in solid solution is important for industrial alloys. In the present work, a solute homogenization model is proposed based on the chemical short-range-order tendency in Mg-Gdbased alloys. After a calculation using the cluster-plus-glue-atom model, the stable Mg-Gd structural unit is derived, [Gd-Mg12]Mg6, where one solute Gd is nearest-neighbored with twelve Mg atoms to form the characteristic hcp cluster [Gd-Mg12] and this cluster is matched with six Mg glue atoms. Such a local unit is then mixed with [Mg-Mg12]Mg3, the stable unit for pure Mg. Assuming that the Gd-containing units are arranged in fcc-or bcc-like lattice points and the Mg units in their octahedral interstices, three proportions between the two units are obtained, 1:1, 2:3, and 1:3, which constitute three solute homogenization modes. The prevailing Mg-Gd-based alloys are consequently classified into three groups, respectively exemplified by GW103 K(Mg-10 Gd-3 Y-0.4 Zr, wt%), GW83 K(Mg-8 Gd-3 Y-0.4 Zr), and GW63 K(Mg-6 Gd-3 Y-0.4 Zr). Mg-Gd-Y-Zr alloys were designed following the model(where Y and Zr were also added in substitution for Gd) and prepared by permanent-mould casting. According to their mechanical properties,the 1:3 alloy(Mg-5.9 Gd-1.6 Y-0.4 Zr) shows the best comprehensive properties(ultimate tensile strength305 MPa, yield strength 186 MPa, elongation 9.0%) in solution plus ageing state. | Shengnan Qian Chuang Dong Tianyu Liu Ying Qin Qing Wang Yujuan Wu Lidong Gu Jianxin Zou Xiangwen Heng Liming Peng Xiaoqin Zeng | 2018 | Journal of Materials Science & Technology2018，34，7： | 6 |
| 7 | Investigation of the alloying effect on deformation behavior in Mg by Visco-Plastic Self-Consistent modeling显示文摘Alloying elements can drastically alter the deformation behavior of Mg.In the present work,Visco-Plastic Self-Consistent(VPSC)modeling was employed to investigate the effect of alloying elements on Mg’s tensile behavior,in particular the relative activity of different slip and twinning modes.Mg-0.47 wt.%Ca,Mg-2 wt.%Nd,and AZ31 extruded alloys were deformed by micro-tensile tests in a scanning electron microscope(SEM).Texture and grain size measured by electron backscatter diffraction(EBSD)were used as the input for VPSC.After parameter optimization,the VPSC model successfully reproduced the stress-strain curve of each alloy.Simulation results indicate that the slip/twinning activity in the three alloys are different.Mg-0.47 wt.%Ca shows strong extrusion texture,and prismatic slip was quite active during its tensile deformation.In contrast,Mg-2 wt.%Nd shows weak extrusion texture,and basal slip was dominant.This alloy also developed more twinning activity than the other two alloys.AZ31 shows strong extrusion texture similar as Mg-0.47 wt.%Ca,but prismatic slip was less active in it.The slip/twinning activity revealed by the VPSC model can explain the difference in the tensile behavior of the three alloys. | Alireza Maldar Leyun Wang Gaoming Zhu Xiaoqin Zeng | 2020 | Journal of Magnesium and Alloys2020，8，1： | 5 |
| 8 | Grain-scale deformation in a Mg-0.8wt% Y alloy using crystal plasticity finite element method显示文摘Magnesium(Mg) alloys with hexagonal close-packed(HCP) structure usually have a poor ductility at room temperature. The addition of yttrium(Y) can improve the ductility of Mg alloys. To understand the underlying mechanism, crystal plasticity finite element method(CPFEM) was employed to simulate the tensile deformation of a Mg-0.8 wt% Y alloy. The simulated stress–strain curve and the grain-scale slip activities were compared with an in-situ tensile test conducted in a scanning electron microscope.According to the CPFEM result, basal slip is the dominant deformation mode in the plastic deformation stage, accounting for about 50% of total strain. Prismatic slip and pyramidal a slip are responsible for about 25% and 20% of the total strain, respectively. Pyramidal c + a slip and twinning, on the other hand,accommodate much less strain. | Wenxue Li Leyun Wang Bijin Zhou Chuanlai Liu Xiaoqin Zeng | 2019 | Journal of Materials Science & Technology2019，35，10： | 5 |
| 9 | Basal-plane stacking-fault energies of Mg alloys: A first-principles study of metallic alloying effects显示文摘Generalized stacking-fault energies(GSFEs) of basal-plane stacking faults I1 and I2 in Mg alloys have been studied based on first-principles calculations, where 43 alloying elements were considered. It is found that the most contributing features of alloying elements to GSFEs are bulk modulus, equilibrium volume,binding energy, atomic radius and ionization energy. Both bulk modulus and ionization energy exhibit positive relationships with GSFEs, and the others show opposite relationships. Multiple regressions have been performed to offer a quantitative prediction for basal-plane GSFEs in Mg-X systems. GSFEs, alloying effects of elements and the prediction model established within this work may provide guidelines for new Mg alloys design with better ductility. | Qing Dong Zhe Luo Hong Zhu Leyun Wang Tao Ying Zhaohui Jin Dejiang Li Wenjiang Ding Xiaoqin Zeng | 2018 | Journal of Materials Science & Technology2018，34，10： | 4 |
| 10 | Microstructural evolution of Mg-Al-Re alloy reinforced with alumina fibers显示文摘Few studies were reported on the phases'relationships of AE44(Mg-4.0Al-4.1RE-0.3Mn,wt.%)and its composites.In this work,AE44 alloy and Saffil(6-Al2O3)/AE44 Metal matrix composite(MMC)were both prepared by slow shot high pressure die casting(SS-HPDC)technology and their phase constitutions were all studied in detail using experimental techniques combined with CALPHAD(Calculation of Phase Diagram)modeling.The results revealed that the alloy consists of the a-Mg matrix,A1hRE3 intermetallic phase,and one trace phase AI3RE,while the composite contains five major phases:a-Mg,5-AI2O3,AI3RE,MgO and Mg2Si.and two trace phases of A12RE and AI11RE3,respectively.A1hRE3 is partly derived from ALRE,while A13RE is a product of the peritectoid reaction between the two precipitates.The presence of MgO and Mg2Si is due to the interfacial reaction between the SiO2 binder in the fiber preforms and the molten magnesium during infiltration.The use of SiO2 binder in the preform manufacturing was limited/minimized to reduce the MgO formation in the MMC casting process,which can be detrimental to the fatigue performance of the MMC materials. | Li Li Dejiang Li Xiaoqin Zeng Alan A.Luo Bin Hu Anil K.Sachdev Liangliang Gu Wenjiang Ding | 2020 | Journal of Magnesium and Alloys2020，8，3： | 4 |
| 11 | Composition optimization of high-strength Mg-Gd-Y-Zr alloys based on the structural unit of Mg-Gd solid solution显示文摘Mg-Gd-Y-Zr alloys with high strength fall within narrow composition range.The present paper explains their composition rule by establishing the cluster-plus-glue-atom unit of Gd-containing Mg solid solution with the aid of Mg matrix and Mg_(5) Gd precipitate phase.First,based on the structural homologue between Gd-containing Mg solid solution and Mg_(5) Gd precipitate phase and in combination with our previously established method for calculating the glue atoms,[Gd-Mg_(12)]Mg_(5) is obtained as the chemical unit of Gd-containing Mg solid solution.Then,seven compositions are designed using different combinations of this unit and that of pure Mg[Mg-Mg_(12)Mg3.After a systematic experimental investigation on the microstructure and mechanical property evolutions as a function of the unit proportions,it is revealed that the Mg-10.1 Gd-3.3 Y-0.9 Zr alloy,being issued from equi-proportion mixing of the two units,shows the strongest tendency of precipitation and reaches the highest strength of 374 MPa after aging.The composition and strength of this alloy are quite close to GW103 K which is well recognized for its general mechanical performance in Mg-Gd-Y-Zr system. | Rui Jiang Shengnan Qian Chuang Dong Ying Qin Yujuan Wu Jianxin Zou Xiaoqin Zeng | 2021 | Journal of Materials Science & Technology2021，，13： | 4 |
| 12 | A New Cockroach Colony Optimization Algorithm for Global Numerical Optimization显示文摘Inspired by the behavior of cockroaches in nature, this paper presents a new optimization algorithm called Cockroach colony optimization(CCO). In the CCO algorithm, nests of cockroaches are placed at the'corner' of the search space. The current best solution to the optimization problem called food can split some of the search targets by applying the logistic multi-peak map and the margin control strategies. By using a particular search scheme, the individual cockroaches can accomplish a highly efficient global and local search in each crawling process from a nest to a search target. The paper provides a formal convergence proof for the CCO algorithm. Experiment results show that the CCO algorithm can be applied to solve global numerical optimization problems with the characteristics of quick convergence and high precision. | CHENG Le HAN Lixin ZENG Xiaoqin BIAN Yuetang | 2017 | Chinese Journal of Electronics2017，26，1： | 3 |
| 13 | Hot compressive deformation behaviors of Mg–10Gd–3Y–0.5Zr alloy显示文摘In this paper, the hot compressive deformation characteristics of a Mg–10Gd–3Y–0.5Zr(GW103K) alloy have been investigated by isothermal compression test at the temperature range of 350–450°C and strain rate range of 0.0001–0.1s^(-1). True stress–strain relationships at various strain rates showed the typical strain hardening and softening stage which is indicative of dynamic recrystallization during deformation. The results showed that the peak stress was obviously dependent on temperature and strain rate. A constitutive equation to describe the deformation process was established based on the hyperbolic sine function. The stress exponent n and apparent activation energy Q were determined to be 3.018 and 203.947 k J/mol, respectively. Microstructure investigation showed that dislocation slipping was the dominant deformation mechanism during the hot deformation at all conditions. However, at the temperatures lower than 400 °C and strain rates higher than 0.01 s^(-1), twinning was observed to be activated, which indicated another deformation mechanism. Dynamic recrystallization and dynamic precipitation were found to occur simultaneously under such deformation condition. | Tingting Zheng Dejiang Li Xiaoqin Zeng Wenjiang Ding | 2016 | Progress in Natural Science:Materials International2016，26，1： | 2 |
| 14 | Characterization on the formation of porosity and tensile properties prediction in die casting Mg alloys显示文摘The 3D visualization of the porosity in high-pressure die casting(HPDC)Mg alloys AZ91D and Mg4Ce2Al0.5Mn(EA42)was investigated by X-ray computed tomography.It was demonstrated that the volumetric porosity at the near-gate location for alloy EA42 was significantly higher than that far from the gate location.This difference resulted from the low valid time during intensified casting pressure conditions.Specimens of alloy EA42 exhibited a narrow pore distribution in the side view(~0.5 mm)compared to the wide distribution(~1.8 mm)of alloy AZ91D,which was mainly attributed to the formation mechanism of the defect band.The formation of microporosity in the defect band of alloy EA42 was inhibited because of the significant latent heat released by a large amount of the Al11Ce3phase segregated in the defect band during solidification.Additionally,an effective estimator(Z-Propagation)was introduced,which is proposed to predict the projected area fraction of the porosity(f)involved during tensile failure with better effectiveness compared with traditional methods based on the actual fractured surface.By coupling the Z-Propagation method with the critical local strain model,the logarithmic fracture strain and true fracture stress of the specimens were predicted within 3.03%and 1.65%of the absolute value of the average relative error(AARE),respectively. | Zixin Li Dejiang Li Wenke Zhou Bo Hu Xingfeng Zhao Jingya Wang Ming Qin Jiangkun Xu Xiaoqin Zeng | 2022 | Journal of Magnesium and Alloys2022，10，7： | 2 |
| 15 | Origins of high ductility exhibited by an extruded magnesium alloy Mg-1.8Zn-0.2Ca:Experiments and crystal plasticity modeling显示文摘Low ductility and strength are major bottlenecks against Mg alloys’wide applications.In this work,we systematically design the composition and fabrication process for a low-alloyed Mg-Zn-Ca alloy,showing that it can be extruded at low temperatures(~250℃)and high speeds(~2 mm/s).After the extrusion,this alloy exhibits a substantially weakened basal texture,relatively small grain size,very high tensile elongation(~30%),and good strength.The origin of the considerably improved ductility was studied using a combination of three-dimensional atom probe tomography(3D-APT),transmission electron microscopy(TEM),electron backscattered diffraction(EBSD)in conjunction with surface slip trace analysis,in-situ synchrotron X-ray diffraction,and elasto-plastic self-consistent(EPSC)modeling.Co-segregation of Zn and Ca atoms at a grain boundary is observed and associated with texture weakening and grain boundary mediated plasticity,both improving the ductility.While basal slip and prismatic slip are identified as the dominant deformation systems in the alloy,the ratio between their slip resistances is substantially reduced relative to pure Mg and most other Mg alloys,significantly contributing to the improved ductility of the alloy.This Mg-Zn-Ca alloy exhibiting excellent mechanical properties and low fabrication cost is a promising candidate for industrial productions. | Jie Wang Gaoming Zhu Leyun Wang Evgenii Vasilev Jun-Sang Park Gang Sha Xiaoqin Zeng Marko Knezevic | 2021 | Journal of Materials Science & Technology2021，，25： | 2 |
| 16 | Effective femtosecond laser shock peening on a Mg-3Gd alloy at low pulse energy 430 μJ of 1 kHz显示文摘In this paper,microstructure evolution and hardness of Mg-3 Gd alloy treated by femtosecond(fs)laser shock peening(LSP)with direct and confined ablation m odes were investigated in detail.Under a relatively low pulse energy of 430μJ with a repetition of 1 kHz,the surface hardness of sample has been enhanced by 70%effectively.Comp a r e d with ns-LSP with pulse fluence of 71.7J/cm2,fs-LSP with pulse fluence of 34.2 J/cm2 is superior in the hardness increment,both of which are in the same order of magnitude.A distinct grain refinement of surface layer has been discovered and results in the increase of hardness.Nonuse of absorption and confining layers and the employment of the industry commercial fs laser with high repetition can inspire big potential L S P application in special metal material.©2019 Published by Elsevier B.V.on behalf of Chongqing University. | Chenghao Lu Licheng Ge Bing Zhu Yangxin Li Xianfeng Chen Xiaoqin Zeng Yuping Chen | 2019 | Journal of Magnesium and Alloys2019，7，3： | 2 |
| 17 | Approximate computation of Madaline sensitivity based on discrete stochastic technique显示文摘The computation of the sensitivity of a Madaline's output to its parameter perturbation is system- atically discussed. Firstly, according to the discrete feature of Adalines, a method based on discrete stochastic technique is proposed, which derives some analytical formulas for the computation of Adalines' sensitivity. The method can theoretically solve some problems that are unsolvable by the existing methods based on continu- ous stochastic techniques, release some unpractical constraints, and make it available to theoretically analyze the approximation error of Adalines' sensitivity. Secondly, on the basis of the sensitivity of Adalines and the structural characteristics of Madalines, a new selection strategy depending on a type of dedication degree for computing Madalines' sensitivity is proposed, which is superior to current popular way of simply averaging in both precision and complexity. The proposed formulas and algorithm have the advantages of simplicity, low computational complexity, small approximation error, and high generality, as have been verified by a great amount of experimental simulations. | ZHONG ShuiMing , ZENG XiaoQin, LIU HuiYi & XU Yan Institute of Pattern Recognition and Intelligent System, College of Computer and Information Engineering, Hohai University, Nanjing 210098, China | 2010 | Science China(Information Sciences)2010，53，12： | 2 |
| 18 | The effect of basal dislocation on {11■1} twin boundary evolution in a Mg-Gd-Y-Zr alloy显示文摘This study aims to understand the features of{11■1}twin boundaries in a high strain rate compressed Mg-10 Gd-3Y-0.4Zr using electron backscatter diffraction(EBSD)and transmission electron microscopy(TEM).Based on the Schmid factor calculations,the basal dislocations are easily activated within the{11■1}twin and suppressed in the surrounding matrix.The consequent basal dislocation-twin interaction during deformation can lead to the misorentiation deviation of{11■1}twin boundaries,accompanying with the formation of a step at the twin boundary.Such a{11■1}twin boundary evolution mechanism provides a new vision of twinning behavior in Mg alloys. | Huan Zhang Yangxin Li Yuxuan Liu Qingchun Zhu Xixi Qi Gaoming Zhu Jinhui Wang Peipeng Jin Xiaoqin Zeng | 2021 | Journal of Materials Science & Technology2021，，22： | 2 |
| 19 | Adaptive Cockroach Colony Optimization for Rod-Like Robot Navigation显示文摘 | Le Cheng Lixin Han Xiaoqin Zeng Yuetang Bian Hong Yan | 2015 | Journal of Bionic Engineering2015，12，2： | 2 |
| 20 | MULTI-FLORET SPIKELET 4(MFS4)Regulates Spikelet Development and Grain Size in Rice显示文摘In rice,the spikelet is the basic unit of inflorescence,and its development is important for determining the grain yield and quality.We reported a rice spikelet mutant multi-floret spikelet 4(mfs4)which resulted in the production of extra floral organs or a whole extra floret,and elongated sterile lemmas.The results suggested that the mutation of the MFS4 gene interfered with spikelet meristem determinacy and floral organ identity.In addition,the plant height and the grain length and width in the mfs4 mutant were all less than those in the wild type.Using the bulked segregant analysis method,the MFS4 gene was localized in a 557-kb region on the long arm of chromosome 1.Sequence analysis showed that there was a C-base deletion at the open reading frame of LOC_Os01g67430.Further tests indicated that a wild type copy of LOC_Os01g67430 was able to reverse the mfs4 defects,which indicated that LOC_Os01g67430 was the MFS4 gene.The MFS4 gene encodes a lipase located in the mitochondria and is expressed strongly in the young inflorescence.qRT-PCR results showed that the expression of some genes that were known to regulate spikelet meristem determinacy and grain size were decreased in the mfs4 mutant,which indicated that the MFS4 gene regulates spikelet meristem determinacy and grain size by modulating the expression of these genes. | Wang Yan Zeng Xiaoqin Lu Lu Cheng Qinglan Yang Fayu Huang Mingjiang Xiong Mao Li Yunfeng | 2021 | Rice science2021，28，4： | 1 |
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