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| 1 | Co-location of QTL for Sclerotinia stem rot resistance and flowering time in Brassica napus显示文摘Sclerotinia stem rot(SSR) caused by Sclerotinia sclerotiorum(Lib.) de Bary is one of the most devastating diseases of Brassica napus worldwide. Both SSR resistance and flowering time(FT) adaptation are major breeding goals in B. napus. However, early maturing rapeseed varieties, which are important for rice-rapeseed rotation in China, are often highly susceptible to SSR. Here, we found that SSR resistance was significantly negatively correlated with FT in a natural population containing 521 rapeseed inbred lines and a double haploid(DH) population with 150 individual lines, both of which had great variation in FT. Four chromosomal regions on A2, A6, C2, and C8 affecting both SSR resistance and FT were identified using quantitative trait loci(QTL) mapping after constructing a high-density genetic map based on single nucleotide polymorphism markers in the DH population.Furthermore, we aligned QTL for the two traits identified in the present and previous studies to the B. napus reference genome, and identified four colocalized QTL hotspots of SSR resistance and FT on A2(0–7.7 Mb), A3(0.8–7.5 Mb), C2(0–15.2 Mb), and C6(20.2–36.6 Mb). Our results revealed a genetic link between SSR resistance and FT in B.napus, which should facilitate the development of effective strategies in both early maturing and SSR resistance breeding and in map-based cloning of SSR resistance QTL. | Jian Wu Peipei Chen Qing Zhao Guangqin Cai Yue Hu Yang Xiang Qingyong Yang Youping Wang Yongming Zhou | 2019 | The Crop Journal2019，7，2： | 1 |
| 2 | The slope creep law for a soft rock in an open-pit mine in the Gobi region of Xinjiang, China显示文摘The lithology of the strata in the Gobi region of the Xinjiang autonomous region of China is mainly composed of mudstone,silty mudstone,and other soft rocks.Because of the low strength of the rock mass and the serious effects of physical weathering in this area,the slope stability in open-pit mines is poor,and creep deformation and instability can readily occur.Taking the Dananhu No.2 open-pit mine as a typical example,the creep test of a mudstone sample under different stress levels was studied.Then,based on a bottom friction experiment and a FLAC3D numerical simulation,the deformation and failure processes of the slope were analyzed.The stress–displacement curve and the displacement–time curve for the monitoring points were plotted to obtain the relationship between the stress and displacement for the slope of the soft rock.The results showed that the long-term strength of the mudstone was between 8.0 and 8.8 MPa,and that stable creep occurred when the slope was under low stress.The potential failure mode for this type of slope is that the front edge creeps along the weak layer and then a crack is formed at the trailing edge of the slope.When the crack penetrates the weak layer,cutting bedding and bedding sliding occur.The deformation process of the stable creep slope includes an initial deformation stage,an initial creep stage,a constant velocity creep stage and a deceleration creep stage. | Hongze Zhao Yu Tian Qingyong Guo Meijie Li Jianwen Wu | 2020 | International Journal of Coal Science & Technology2020，7，2： | 1 |
| 3 | In vitro and in vivo evaluation of camptothecin nanosuspension: a novel formulation with high antitumor efficacy and low toxicity显示文摘 | YAO Liping ZHAO Xiuhua LI Qingyong | 2012 | lnt JPharm2012，423，2： | 1 |
| 4 | Additive Effects of QTLs/Genes on Rice Grain Size Traits Revealed by Genetic Comparisons显示文摘Grain size is controlled by many QTLs and/or genes.Despite intensive study,the genetic interactions between these QTLs/genes remain largely unclear.We previously found that the japonica large-grain rice cultivar TD70 with 1000-grain weight of 68.6 g carries superior alleles at the loci GW2,GS3,qGL3,GS5 and GW8,all of which are known to control grain size,while the indica rice variety Kasalath with 1000-grain weight of 19.1 g has normal alleles at these loci. | HE Lei LIANG Wenhua HU Jiang ZHAO Chunfang YAO Shu CHEN Tao ZHU Zhen ZHAO Qingyong LU Kai ZHAO Ling ZHOU Lihui QIAN Qian WANG Cailin ZHANG Yadong | 2023 | Rice science2023，30，3： | 0 |
| 5 | Generation of CRISPR/Cas9-mediated lactoferrin-targeted mice by pronuclear injection of plasmid pX330显示文摘Lactoferrin is a member of the transferrin family of multifunctional iron binding glycoproteins.While numerous physiological functions have been described for lactoferrin,the mechanisms underlying these functions are not clear.To further study the functions and mechanisms of lactoferrin,we modified the lactoferrin promoter of mice using the CRISPR/Cas9 system to reduce or eliminate lactoferrin expression.Seven mice with lactoferrin promoter mutations were obtained with an efficiency of 24%(7/29)by injecting the plasmid pX330,expressing a small guide RNA and human codonoptimized SpCas9,into fertilized eggs of mice.Plasmid integration and off-targeting of pX330 were not detected.These results confirmed that pronuclear injection of a circular plasmid is a feasible and efficient method for targeted mutagenesis in mice. | Mengxu GE Fei LIU Fei CHANG Zhaolin SUN Jing FEI Ying GUO Yunping DAI Zhengquan YU Yaofeng ZHAO Ning LI Qingyong MENG | 2015 | Frontiers of Agricultural Science and Engineering2015，2，3： | 0 |
| 6 | Depletion of conventional mature B cells and compromised specific antibody response in bovine immunoglobulin μ heavy-chain transgenic mice显示文摘In this study,we introduced the bovine immunoglobulinμheavy-chain gene(the orphaned gene on BTA11)into mouse germline cells.Bovine IgM was highly expressed in selected transgenic lines,and it largely inhibited rearrangements of the endogenous immunoglobulin heavy chain(IgH)genes in these lines.The forced expression of bovine IgM resulted in reduced numbers of pro-and pre-B cells but increased the number of immature B cells in the transgenic mice.Bovine IgM-expressing B cells can migrate from the bone marrow to the spleen,but most of the cells are arrested at the T1 transitional B cell stage,leading to a significantly lower number of T2 transitional and mature B cells in the spleen.Although the serum concentrations of endogenous IgM and IgG in the transgenic mice were significantly decreased,the IgA levels were slightly increased compared to the WT mice.The bovine IgM level in the serum was only one-tenth to one-fifth of that of endogenous mouse IgM,suggesting that most of the serum immunoglobulin were contributed by endogenous IgH gene-expressing B cells.These transgenic mice also exhibited a lower frequency of unique complementarity determining region 3(CDR3)sequences in their VH repertoire and Vκrepertoire but exhibited an increased frequency of unique CDR3 in their Vλrepertoire.Compared to the WT mice,the transgenic mice had a significantly higher percentage of mouse IgMexpressing B cells that expressedλchains.Finally,we showed that the transgenic mice were deficient in a specific antibody response to antigen stimulation. | Min ZHANG Xueqian CHENG Dan CHU Jingwen LIANG Yi SUN Li MA Beilei XU Min ZHENG Meili WANG Liming REN Xiaoxiang HU Qingyong MENG Ran ZHANG Ying GUO Yunping DAI Robert AITKEN Ning LI Yaofeng ZHAO | 2014 | Frontiers of Agricultural Science and Engineering2014，1，2： | 0 |
| 7 | Association mapping and domestication analysis to dissect genetic improvement process of upland cotton yield-related traits in China显示文摘Background: Cotton fiber yield is a complex trait,which can be influenced by multiple agronomic traits.Unravelling the genetic basis of cotton fiber yield-related traits contributes to genetic improvement of cotton.Results: In this study,503 upland cotton varieties covering the four breeding stages(BS1–BS4,1911–2011)in China were used for association mapping and domestication analysis.One hundred and forty SSR markers significantly associated with ten fiber yield-related traits were identified,among which,29 markers showed an increasing trend contribution to cotton yield-related traits from BS1 to BS4,and 26 markers showed decreased trend effect.Four favorable alleles of 9 major loci(R^(2)≥3)were strongly selected during the breeding stages,and the candidate genes of the four strongly selected alleles were predicated according to the gene function annotation and tissue expression data.Conclusions :The study not only uncovers the genetic basis of 10 cotton yield-related traits but also provides genetic evidence for cotton improvement during the cotton breeding process in China. | GUO Chunping PAN Zhenyuan YOU Chunyuan ZHOU Xiaofeng HUANG Cong SHEN Chao ZHAO Ruihai YANG Qingyong ZHU Longfu SHAHZAD Raheel MENG Fande LIN Zhongxu NIE Xinhui | 2021 | Journal of Cotton Research2021，4，2： | 0 |
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