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| 1 | Effects of applying lactic acid bacteria to the fermentation on a mixture of corn steep liquor and air-dried rice straw显示文摘This study was to determine the fermentation quality of a mixture of corn steep liquor(CSL)(178 g/kg wet basis) and air-dried rice straw(356 g/kg wet basis) after being treated with inoculants of different types of lactic acid bacteria(LAB). The treatments included the addition of no LAB additive(control),which was deionized water; homo-fermentative LAB alone(^(ho)LAB), which was Lactobacillus plantarum alone), and a mixture of homo-fermentative and hetero-fermentative LAB(^(he+ho)LAB), which were L. plantarum, Lactobacillus casei, and Lactobacillus buchneri. The results showed that the inoculation of the mixture of CSL and air-dried rice straw with ^(he+ho)LAB significantly increased the concentration of acetic acid and lactic acid compared with the control(P < 0.05). The addition of ^(he+ho)LAB effectively inhibited the growth of yeast in the silage. The concentration of total lactic acid bacteria in the ^(he+ho)LAB-treated silage was significant higher than those obtained in other groups(P < 0.05). The duration of the aerobic stability of the silages increased from 56 h to >372 h. The control group was the first to spoil, whereas the silage treated with ^(he+ho)LAB remained stable throughout the 372 h period of monitoring. The results demonstrated that the ^(he+ho)LAB could effectively improve the fermentation quality and aerobic stability of the silage. | Xinxin Li Wenbin Xu Jinshan Yang Hongbo Zhao Chunfang Pan Xue Ding Yonggen Zhang | 2016 | Animal Nutrition2016，，3： | 17 |
| 2 | Human monoclonal antibodies block the binding of SARS-CoV-2 spike protein to angiotensin converting enzyme 2 receptor显示文摘According to the World Health Organization(WHO)newly updated situation report on March 18th,2020,the coronavirus disease 2019(COVID-19)pandemic has confirmed 191,127 cases and claimed 7807 deaths worldwide.1 The etiological agent of COVID-19 has been identified as a novel coronavirus,the severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),belonging to Sarbecovirus subgenus(genus Betacoronavirus,family Coronaviridae)and showing 79.6 and 96.2%sequence identity in nucleotide to SARS-CoV and a bat coronavirus(BatCoV RaTG13),respectively.2–4 Like SARS-CoV infection,a substantial fraction of COVID-19 patients exhibits severe respiratory symptoms and has to be hospitalized in intensive care unit.5–8 Although the mortality rate of COVID-19 is significantly lower than that of SARS-CoV infection,SARS-CoV-2 shows much higher human-to-human transmission rate,rapidly leading to a global pandemic declared by WHO on March 11th,2020. | Xiangyu Chen Ren Li Zhiwei Pan Chunfang Qian Yang Yang Renrong You Jing Zhao Pinghuang Liu Leiqiong Gao Zhirong Li Qizhao Huang Lifan Xu Jianfang Tang Qin Tian Wei Yao Li Hu Xiaofeng Yan Xinyuan Zhou Yuzhang Wu Kai Deng Zheng Zhang Zhaohui Qian Yaokai Chen Lilin Ye | 2020 | Cellular & Molecular Immunology2020，17，6： | 17 |
| 3 | Laboratory Characterizations on 2007 Cases of Monoclonal Gammopathies in East China显示文摘Monoclonal gammopathies are characterized by the presence of monoclonal immunoglobulin in patients with or without evidence of multiple myeloma (MM),macroglobulinemia,amyloidosis (AL),or a related plasma cell proliferative disorder. This study aims to evaluate laboratory diagnostic characters of monoclonal gammopathies and investigates the correlation between monoclonal gammopathies and transforming growth factor β1 (TGFβ1). Immunofixation electrophoresis (IFE),serum protein electrophoresis (SPE),nephelometry and urine light chain ELISA were used for laboratory identification of monoclonal immunoglobulins. Plasma TGFβ1 was detected with double-antibodies ELISA. Lightcycler was used for single nucleotide polymorphism (SNP) analysis. Totally 2,007 cases of monoclonal immunoglobulin (M protein) were identified in 10,682 samples. The isotypes of M protein were IgG type 47.1%,IgA 23.0%,IgM 8.7%,IgD 5.3%,free light chain κ 6.1%,λ 9.8%. In reference to IFE,the coherency of diagnosis was serum light chain ratio (κ/λ) 94.4%,quantitation of Igs 83%,light chain quantitation 80.9%,and urine light chain ratio (κ/λ) 58.0%. Plasma TGFβ1 was elevated significantly compared to normal control. The allelic frequency of codon 10 (C > T) was neither associated with the existence of the M protein nor with the M protein isotype. Monoclonal gammopathies can be identified with the combination of IFE,SPE,Igs quantitaion and urine light chain determination. Although TGFβ1,an important cytokine in immune regulation,was elevated in monoclonal gammopathies,the SNPs in coding region of TGFβ1 gene did not confer susceptibility to the development of monoclonal gammopathies in this study. | Hao Wang Chunfang Gao Lingling Xu Zaixing Yang Wenjing Zhao Xiantao Kong | 2008 | Cellular & Molecular Immunology2008，5，4： | 8 |
| 4 | Fine Mapping of RppP25, a Southern Rust Resistance Gene in Maize显示文摘Southern rust (Puccinia polysora Underw.) is a major disease that can cause severe yield losses in maize (Zea mays L.). In our previous study, a major gene RppP25 that confers resistance to southern rust was identified in inbred line P25. Here, we report the fine mapping and candidate gene analysis of RppP25 from the near-isogenic line F939, which harbors RppP25 in the genetic background of the susceptible inbred line F349. The inheritance of resistance to southern rust was investigated in the BC1F1 and BC3F1 populations, which were derived from a cross between F939 and F349 (as the recurrent parent). The 1:1 segregation ratio of resistance to susceptible plants in these two populations indicated that the resistance is controlled by a single dominant gene. Ten markers, including three simple sequence repeat (SSR) markers and seven insertion/deletion (InDel) markers, were developed in the RppP25 region. RppP25 was delimited to an interval between P091 and M271, with an estimated length of 40 kb based on the physical map of B73. In this region, a candidate gene was identified that was predicted to encode a putative nucleotide-binding site leucine-rich repeat (NBS-LRR) protein. Two co-segregated markers will aid in pyramiding diverse southern rust resistance alleles into elite materials, and thereby improve southern rust resistance worldwide. | Panfeng Zhao Guobin Zhang Xiaojun Wu Na Li Dianyi Shi Dengfeng Zhang Chunfang Ji Mingliang Xu Shoucai Wang | 2013 | Journal of Integrative Plant Biology2013，55，5： | 5 |
| 5 | Repositioning techniques of malpositioned peripherally inserted central catheters显示文摘 | Jingfen Jin Chunfang Chen Ruiyi Zhao | | 0，，13： | 1 |
| 6 | MicroRNA-101 suppresses SOX9-dependent tumorigenicity and promotes favorable prognosis of human hepatocellular carcinoma显示文摘 | Yanqiong Zhang<.--Q1: Please confirm that given names and surnames have been identified correctly.--> Xiaodong Guo Lu Xiong Xiangying Kong Ying Xu Chunfang Liu Lin Zou Zhiwei Li Jingmin Zhao Na Lin | 2012 | FEBS Letters2012，，： | 1 |
| 7 | MicroRNA-101 suppresses SOX9-dependent tumorigenicity and promotes favorable prognosis of human hepatocellular carcinoma显示文摘 | Yanqiong Zhang< !--Q1: Please confirm that given names and surnames have been identified correctly.--> Xiaodong Guo Lu Xiong Xiangying Kong Ying Xu Chunfang Liu Lin Zou Zhiwei Li Jingmin Zhao Na Lin | 2012 | FEBS Letters2012，，24： | 1 |
| 8 | Additive Effects of QTLs/Genes on Rice Grain Size Traits Revealed by Genetic Comparisons显示文摘Grain size is controlled by many QTLs and/or genes.Despite intensive study,the genetic interactions between these QTLs/genes remain largely unclear.We previously found that the japonica large-grain rice cultivar TD70 with 1000-grain weight of 68.6 g carries superior alleles at the loci GW2,GS3,qGL3,GS5 and GW8,all of which are known to control grain size,while the indica rice variety Kasalath with 1000-grain weight of 19.1 g has normal alleles at these loci. | HE Lei LIANG Wenhua HU Jiang ZHAO Chunfang YAO Shu CHEN Tao ZHU Zhen ZHAO Qingyong LU Kai ZHAO Ling ZHOU Lihui QIAN Qian WANG Cailin ZHANG Yadong | 2023 | Rice science2023，30，3： | 0 |
| 9 | Classification of archaic rice grains excavated at the Mojiaoshan site within the Liangzhu site complex reveals an Indica and Japonica chloroplast complex显示文摘To understand rice types that were utilized during postdomestication and in the modern age and the potential of genetic research in aged rice materials,archaeogenetic analysis was conducted for two populations of archaic rice grains from the Mojiaoshan site during the Liangzhu Period in China(2940 to 2840 BC).Sequencing after the PCR amplification of three regions of the chloroplast genome and one region of the nuclear genome showed recovery rates that were comparable to those in previous studies except for one chloroplast genome region,suggesting that the materials used in this work were appropriate for recovering genetic information related to domestication traits by using advanced technology.Classification after sequencing in these regions proved the existence of Japonica and Indica chloroplasts in archaic grains from the west trench,which were subsequently classified into eight plastid groups(type I–VIII),and indicated that these rice grains derived from different maternal lineages were stored together in storage houses at the Mojiaohsan site.Among these plastid groups,type V exhibited the same sequences as two modern Indica accessions that are utilized in basic studies and rice breeding.It was inferred that part of the chloroplast genome of archaic rice has been preserved in modern genetic resources in these two modern Indica accessions,and the results indicated that rice related to their maternal ancestor was present at the Mojiaoshan site during the Liangzhu Period in China.The usefulness of archaeogenetic analysis can be demonstrated by our research data as well as previous studies,providing encouragement for the possibility that archaeogenetic analysis can be applied to older rice materials that were utilized in the rice-domesticated period. | Katsunori Tanaka Chunfang Zhao Ningyuan Wang Shinji Kubota Masaaki Kanehara Nobuhiko Kamijo Ryuji Ishikawa Hiroyuki Tasaki Minako Kanehara Bin Liu Minghui Chen Shin-ichi Nakamura Tetsuro Udatsu Cailin Wang | 2020 | Food Production, Processing and Nutrition2020，2，1： | 0 |
| 10 | Effects of soluble starch synthase genes on eating and cooking quality in semi waxy japonica rice with Wx^(mp)显示文摘The purpose of this study is to reveal the genetic mechanism of the variation of amylose content among different semi waxy or glutinous japonica rice in the background of Wxmp gene.Sixty-four semi waxy lines derived from the hybrid progenies of Wujing 13 and Milky Princess(Kantou 194)with polymorphism in soluble starch synthase gene SSIIa(SSII-3)and SSIIIa(SSIII-2)but no polymorphism in other starch synthase related genes were used as test materials.The genotypes of SSIIa and SSIIIa allele were identified by molecular markers,and the allelic effects of SSIIa and SSIIIa gene on amylose content(AC),gel consistency(GC),gelatinization temperature(GT)and rapid visco analyzer(RVA)profile characteristics were analyzed.The significant effects of SSIIa and SSIIIa alleles and the interactive effects between two genes on AC,GT,GC and RVA profile characteristics were found.The SSIIa and SSIIIa alleles from Wujing13 shown positive effects on AC with an average increase of 1.87 and 1.23%in 2 years respectively.There was no significant effect on GT for SSIIa or SSIIIa allele but remarkable influence on GT when the co-existence of the two genes.The genotype SSIIa^(mp)SSIIIa^(mp) shown 1.34℃higher GT than genotype SSIIawjSSIIIawj(mp and wj indicated that the gene was derived from Milky Princess and Wujing 13 respectively,the same as in the below).Different genes and alleles resulted in significant different GC.The genetic effect of SSIIawj and SSIIIamp on GC was 8.74 and 9.62mm respectively.The GC of SSIIa^(wj)SSIIIa^(mp) was 10.64 and 16.95mm higher than that of SSIIa^(mp)SSIIIa^(wj) and SSIIawjSSIIIawj,respectively.The allele SSIIa^(wj) could increase the peak viscosity(PKV),hot paste viscosity(HPV),cool paste viscosity(CPV)and breakdown viscosity(BDV),while decrease the consistency viscosity(CSV)and setback viscosity(SBV).However for the allele SSIIIa^(wj) the opposite was true.The genotype SSIIa^(wj)SSIIIa^(mp) had the largest PKV,HPV and CPV,the genotype SSIIa^(wj)SSIIIa^(wj) had the largest BDV and CSV,but the genotype SSIIa^(wj)SSIIIa^(mp) had the least SBV.According to the comprehensive effect of each trait,the genotype SSIIawjSSIIIamp was the best.The allelic variation and interaction effect of SSIIa and SSIIIa genes have important reference value for improving cooking and eating quality of semi waxy japonica rice. | Shu Yao Yadong Zhang Yanqing Liu Chunfang Zhao Lihui Zhou Tao Chen Qing Yong Zhao Balakrishna Pillay Cailin Wang | 2020 | Food Production, Processing and Nutrition2020，2，1： | 0 |
| 11 | Mesenchymal stem cells:As a multi-target cell therapy for clearingβ-amyloid deposition in Alzheimer’s disease显示文摘Extracellularβ-amyloid(Aβ)plaques and neurofibrillary tangles(NFTs)are the pathological hallmarks of Alzheimer’s disease(AD).Studies have shown that aggregates of extracellular Aβcan induce neuroinflammation mediated neurotoxic signaling through microglial activation and release of pro-inflammatory factors.Thus,modulation of Aβmight be a potential therapeutic strategy for modifying disease progression.Recently,a large number of reports have confirmed the beneficial effects of mesenchymal stem cells(MSCs)on AD.It is believed to reduce neuroinflammation,reduce Aβamyloid deposits and NFTs,increase acetylcholine levels,promote neurogenesis,reduce neuronal damage,and improve working memory and cognition.In this review,we focus on the role of MSCs in clearing Aβdeposition.MSCs have the potential to modulate Aβ-related microenvironments via enhancement of autophagy,proteolysis of Aβaggregates,phagocytic clearance of Aβby microglial M2 polarization,decrease oxidative stress(OS),and correction of abnormal sphingolipid(SL)metabolism.With advantages in clinical applications,these data suggest that the use of MSCs as a multi-target modulator of Aβwould be an effective therapeutic approach in AD. | RUXIN ZHANG CHENGGANG LI RUOCHEN DU YITONG YUAN BICHUN ZHAO YUJUAN ZHANG CHUNFANG WANG | 2022 | BIOCELL2022，46，3： | 0 |
| 12 | Gene expression of granulosa and cumulus cells: The prospect inpredicting the quality and developmental competence of oocytesin vitro maturation显示文摘In vitro maturation(IVM),a promising assisted reproductive technology(ART),has been evolving in clinical trials and applications.There is a huge potential demand for IVM in clinical practice because it reduces the stimulation of gonadotropins to patients and provides evidence for the safety of neonatal birth.Unfortunately,the maturation rate of oocytes in vitro is not as high as it is in vivo due to a different microenvironment.Moreover,there are still controversies in predicting the developmental capability of oocytes in IVM.The granulosa cells(GCs)and cumulus cells(CCs),closely surrounding the oocytes,play a critical role in oocytes development,while some studies have shown that they can reflect the quality of oocytes.Many studies have been conducted in terms of oocyte quality in transcriptional level in GCs and CCs of mice,Xenopus africanus,and Homo sapiens,which provides important enlightenment for the successful clinical application of IVM.However,no comprehensive reviews about how gene expression profiles affect oocytes quality have been reported.This review aimed to elucidate the gene expression profiles of GCs and CCs that have effects on the quality and developmental competence of oocytes maturation in vitro.And we also put forward a possible idea for ART in the future,integrating all gene expression profiles of GCs and CCs and predicting the quality of the oocytes. | BICHUN ZHAO XUEQING WU YITONG YUAN YUANTAO GAO XIAO LI RUOCHEN DU SUMING XU RUXIN ZHANG CHUNFANG WANG | 2020 | BIOCELL2020，44，4： | 0 |
| 13 | Establishment of a real-time PCR for quantifying transforming growth factor beta1 in blood of hepatocellular carcinoma patients显示文摘Background: The carcinogenesis of hepatocellular carcinoma (HCC) is a multi-factorial, multistep and complex process. Its prognosis is poor and early detection is of the utmost importance. Transforming growth factor β1 (TGF-β1) message RNA (mRNA) has been reported to be elevated in HCC patients using Northern blotting. However, little work has been done about the detection of TGF-β1 mRNA levels in peripheral blood of patients with HCC using the real-time polymerase chain reactions (PCR) method. Objective: To assess the prognostic value of quantitative levels of TGF-β1 mRNA in peripheral blood of patients with HCC, and to investigate the relationship between the expression of TGF-β1 mRNA in peripheral blood and many diagnostic and pathological factors. Methods: We developed an optimized Taqman real-time PCR to quantify TGF-β1 mRNA in peripheral blood of 53 patients with HCC and 44 healthy volunteers. In addition, blood was collected from patients with HCC for measuring levels of total bilirubin (TBil), prealbumin, albumin, alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma glutamyltranspeptidase (GGT), alpha-L-fucosidase (AFU), alpha fetoprotein (AFP), carcino-embryonic antigen (CEA), carbohydrate antigen 19-9 (CA19-9), viral load and platelet counts. Statistical analysis was performed using the SPSS software system (SPSS 10.0). Results: In real-time PCR, fluorescence was detectable in all blood specimens from patients with HCC and healthy volunteers. The levels of TGF-β1 mRNA expression in patients with HCC were significantly higher compared to that in healthy volunteers (P<0.000 1), suggesting an association of the activated TGF-β1 gene transcription with hepato- carcinogenesis. Patients with HCC were divided into 2 groups according to their TGF-β1 mRNA above (group A, n=28)or below (group B, n=25) the mean level. Statistical results demonstrated that TGF-β1 mRNA expression level was correlated with patients age, serum levels of CEA, CA19-9 and viral copy number (P<0.05). Conclusion: Although this is a small sample size pilot study these findings imply that quantitative measurement of TGF-β1 mRNA level in peripheral blood may be a complementary serologic marker of HCC. | Qi Peng Gao Chunfang Fang Meng Ji Qiang Zhao Yunpeng Liu Yan Sun Xiaojuan | 2008 | Journal of Medical Colleges of PLA(China)2008，23，4： | 0 |
| 14 | The Status Quo of Reverse Logistics in China and Countermeasures显示文摘 | Chunfang Guo Liping Shao Huijun Zhao | 2004 | Chinese Business Review2004，3，7： | 0 |
| 15 | Study of Highly Ionized Atom Ne显示文摘Study of Highly Ionized Atom NeStudyofHighlyIonizedAtomNe￥WangYoude;MaXinwen;LiuHuiping;YangZhihu;ZhaoMengchun;DuYifei;GuoTia... | Wang Youde Ma Xinwen Liu Huiping Yang Zhihu Zhao Mengchun Du Yifei Guo Tianrui and Wang Chunfang | 1995 | IMP & HIRFL Annual Report1995，，0： | 0 |
| 16 | Rapid Microwave Assisted Synthesis and Antiproliferative Evaluation of Novel Steroidal Thiazole Derivatives显示文摘Three series of novel steroidal thiazole derivatives were synthesized by microwave assisted one-potreaction from pregnenolone, testosterone and estrone, respectively. Their structures were characterized by IR, NMRand HRMS, and the antiproliferative activities of all the synthesized compounds against human cervical carcinoma(HeLa), human liver carcinoma(HepG2), human lung carcinoma(A549), nasopharyngeal carcinoma(CNE2) andnormal kidney epithelial cells(HEK293T) were screened. Among the compounds, 1-estron-17'-ylidene-2-[4'-(p-bromophenyl)-2'-thiazol]hydrazone(12) displayed distinct antiproliferative activity against all the tested cancercell lines and was almost inactive to normal kidney epithelial cells. | HUANG Yanmin ZHAO Dandan LIU Chang GAN Chunfang ZHAN Junyan LIN Qifu SHI Haixin CUI Jianguo | 2017 | Chemical Research in Chinese Universities2017，33，2： | 0 |
| 17 | Improvement of Beamfoil Spectroscope System显示文摘Improvement of Beamfoil Spectroscope SystemImprovementofBeamfoilSpectroscopeSystem￥MaXinwen;DuYifei;GuoTianrui;WangChunfang;W... | Ma Xinwen Du Yifei Guo Tianrui Wang Chunfang Wang Youde Yang Zhihu Liu Huiping and Zhao Mengchun | 1995 | IMP & HIRFL Annual Report1995，，0： | 0 |
| 18 | CR-Fuxing high-speed EMU series显示文摘1Overview of the CR Fuxing high-speed EMUseries The independently developed CR(China Railways)Fuxing high-speed EMU(electric multiple unit)series by China currently comprises three speed-level product series:CR400,CR300,and CR200,as shown in Fig.1.The EMUs demonstrate adaptability to various geographical and climatic conditions,including high plateaus,extremely cold regions,strong wind and sandy environmental conditions,with a designed service life of 30 years.The 350 km/h speed level of the CR-Fuxing series primarily consists of two platforms,namely CR400AF and CR400BF,both of them are electric multiple units with distributed power.Each 8-car formation is configured with a 4M4T arrangement,divided into two traction units(Tc+M+Tp+M). | Chunfang LU Bo ZHANG Hongwei ZHAO | 2023 | Frontiers of Engineering Management2023，10，4： | 0 |
| 19 | Cytokine levels and pathological characteristics of a patient with severe coronavirus disease 2019:a case report显示文摘To the Editor:The clinical spectrum of coronavirus disease 2019(COVID-19)appears to be wide,ranging from asymptomatic to severe progressive pneumonia with respiratory failure,multiorgan failure,and even death.Here,we investigated the clinical and pathological characteristics of a patient who died from severe COVID-19.Our findings will facilitate a deeper understanding of the pathogenesis and progression of COVID-19 and improve clinical strategies to combat the disease.On January 23,2020,a 57-year-old man without relevant history presented with fatigue and fever after attending a family party 2 days prior.Among the members of the family party,one relative had traveled from Wuhan.Over 9 days,the fever and cough developed,and the patient visited the emergency department.A throat swab was positive for severe acute respiratory syndrome coronavirus 2(SARS-COV-2)on real-time reverse-transcription polymerase chain reaction.He was admitted to the local hospital and received supportive therapies,anti-viral agents,atomized inhalation of interferon-γ,and oxygen therapy.Cough,fever,and dyspnea further developed on February 8.He received non-invasive mechanical ventilation therapy.On February 18,he was transferred to a superior hospital with shortness of breath. | Kai Kang Yang Gao Mingyan Zhao Dongsheng Fei Ming Ye Yan Gao Wei Yang Changsong Wang Haitao Liu Guangping Chang Xianxin Kang Yunpeng Luo Xue Du Jiping Qi Lin Tian Min Zhou Chunfang Hao Kaijiang Yu | 2022 | Chinese Medical Journal2022，，1： | 0 |
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