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69篇 您的检索式:作者名="ZHU Junhua"
    题名 作者 年代 出处 被引量
1Programmed Self-Elimination of the CRISPR/Cas9 Construct Greatly Accelerates the Isolation of Edited and Transgene-Free Rice Plants显示文摘Yubing He Min Zhu Lihao Wang Junhua Wu Qiaoyan Wang Rongchen Wang Yunde Zhao 2018Molecular Plant2018,11,9:18
2Traditional Chinese medicine in COVID-19显示文摘COVID-19 pandemic caused by severe acute respiratory syndrome coronavirus 2(SARSCo V-2)has spread across the globe,posing an enormous threat to public health and safety.Traditional Chinese medicine(TCM),in combination with Western medicine(WM),has made important and lasting contributions in the battle against COVID-19.In this review,updated clinical effects and potential mechanisms of TCM,presented in newly recognized three distinct phases of the disease,are summarized and discussed.By integrating the available clinical and preclinical evidence,the efficacies and underlying mechanisms of TCM on COVID-19,including the highly recommended three Chinese patent medicines and three Chinese medicine formulas,are described in a panorama.We hope that this comprehensive review not only provides a reference for health care professionals and the public to recognize the significant contributions of TCM for COVID-19,but also serves as an evidence-based in-depth summary and analysis to facilitate understanding the true scientific value of TCM.Ming Lyu Guanwei Fan Guangxu Xiao Taiyi Wang Dong Xu Jie Gao Shaoqin Ge Qingling Li Yuling Ma Han Zhang Jigang Wang Yuanlu Cui Junhua Zhang Yan Zhu Boli Zhang 2021Acta Pharmaceutica Sinica B2021,11,11:14
3Spatial-temporal variation of phytoplankton community structure in Jiaozhou Bay, China显示文摘To better understand the spatial-temporal variation in phytoplankton community structure and its controlling factors in Jiaozhou Bay, Qingdao, North China, four seasonal sampling were carried out in 2017. The phytoplankton community structure and various environmental parameters were examined. The phytoplankton community in the bay was composed of mainly diatoms and dinofl agellates, and several other species of Chrysophyta were also observed. Diatoms were the most dominant phytoplankton group throughout the year, except in spring and winter, when Noctiluca scintillans was co-dominant. High Si/N ratios in summer and fall refl ect the high dominance of diatoms in the two seasons. Temporally, the phytoplankton cell abundance peaked in summer, due mainly to the high temperatures and nutrient concentrations in summer. Spatially, the phytoplankton cell abundance was higher in the northern part of the bay than in the other parts of the bay in four seasons. The diatom cell abundances show signifi cant positive correlations with the nutrient concentrations, while the dinofl agellate cell abundances show no correlation or a negative correlation with the nutrient concentrations but a signifi cant positive correlation with the stratifi cation index. This discrepancy was mainly due to the diff erent survival strategies between diatoms and dinofl agellates. The Shannon-Wiener diversity index ( H′) values in the bay ranged from 0.08 to 4.18, which fell in the range reported in historical studies. The distribution pattern of H′ values was quite diff erent from that of chlorophyll a , indicating that the phytoplankton community structure might have high biomass with a low diversity index. Compared with historical studies, we believe that the dominant phytoplankton species have been changed in recent years due mainly to the changing environment in the Jiaozhou Bay in recent 30 years.GUO Shujin ZHU Mingliang ZHAO Zengxia LIANG Junhua ZHAO Yongfang DU Juan SUN Xiaoxia 2019Journal of Oceanology and Limnology2019,37,5:6
4Implementing single-scale wavelet transform processor with magnetostatic surface wave device显示文摘When the envelope of the conducting strips for the input transducer of magnetostatic surface wave(MSSW) device is designed according to the wavelet-function envelope,the impulse-response function of the input transducer for MSSW device is equal to the wavelet function,so that single-scale wavelet-transform processor of MSSW type is fabricated.First,according to the envelope function of the wavelet,the length function of the conducting strips is defined,then,the lengths of the conducting strips can be calculated from the length function of the conducting strips,finally,the input transducer can be designed according to the lengths and widths for the conducting strips.The compensation method of the insertion loss for single-scale wavelet trans-form processor of MSSW type is also given in the paper.When the output end of single-scale wavelet transform processor of MSSW type is connected to the amplifier,its insertion loss can be compensated.LU WenKe ZHU ChangChun KUANG Lun ZHANG Ting LIU JunHua 2011Science China(Technological Sciences)2011,54,6:6
5Effect of cDNA fragments in different length derived from Potato Virus Y coat protein gene on the induction of RNA-mediated virus resistance显示文摘We have reported that cDNA derived from entire coat protein (CP) gene of potato virus Y (PVY) could induce resistance to PVY infection in transgenic tobacco plants, and the resistance was further demonstrated to be RNA-mediated rather than coat protein-mediated. In this study, we cloned cDNA fragments of 202 bp, 417 bp, and 603 bp in length derived from the 3′ end of the PVY CP gene, and the cDNA fragments were introduced into tobacco (var. NC89) plants via Agrobacterium-mediated transformation system. The results of resistance assay showed that the CP cDNA fragments of 417 bp and 603 bp could confer resistance of the trans-genic plants to PVY infection, but the fragment of 202 bp in length could not. Molecular analysis revealed that the resistance was RNA-mediated, which is believed to be a result of post-transcriptional gene silencing. The results indicate that the length of cDNA fragments needed for resistance induction was located somewhere between 202 bp and 417 bp from the 3′ end of PVY CP gene.ZHU Junhua ZHU Xiaoping WEN Fujiang BAI Qingrong ZHU Changxiang SONG Yunzhi 2004Science China(Life Sciences)2004,47,4:5
6Morphological adaptation of sheep's rumen epithelium to high-grain diet entails alteration in the expression of genes involved in cell cycle regulation, cell proliferation and apoptosis显示文摘Background: The objectives of this study were to characterize changes in the relative m RNA expression of candidate genes and proteins involved in cell cycle regulation, cell proliferation and apoptosis in the ruminal epithelium(RE) of sheep during high-grain(HG) diet adaptation.Results: Twenty sheep were assigned to four groups with five animals each. These animals were assigned to different periods of HG diet(containing 40% forage and 60% concentrate mix) feeding. The HG groups received an HG diet for7(G7, n = 5), 14(G14, n = 5) and 28 d(G28, n = 5), respectively. In contrast, the control group(CON, n = 5) was fed the forage-based diet for 28 d. The results showed that HG feeding linearly decreased(P < 0.001) the ruminal p H, and increased the concentrations of ruminal total volatile fatty acid(linear, P = 0.001), butyrate(linear, P < 0.001), valerate(quadratic P = 0.029) and the level of IGF-1(quadratic, P = 0.043) in plasma. The length(quadratic, P = 0.004), width(cubic, P = 0.015) and surface of the ruminal papillae(linear, P = 0.003) were all enlarged after 14 d of HG diet feeding.HG feeding cubically increased the number of cell layers forming the stratum corneum(SC, P < 0.001) and the thickness of the SC(P < 0.001) and stratum basale(P < 0.001). The proportion of basal layer cells in the RE decreased(linear, P < 0.001) in the G0/G1-phase, but it increased linearly(P = 0.006) in the S-phase and cubically(P = 0.004) in the G2/M-phases. The proportion of apoptosis cells in G7, G14 and G28 was reduced compared to the CON(quadratic, P <0.001). HG diet feeding linearly decreased the m RNA expression of Cyclin E1(P = 0.021) and CDK-2(P = 0.001) and(P = 0.027) the protein expression of Cyclin E1. Feeding an HG diet linearly increased the m RNA expression of genes IGFBP-2(P = 0.034) and IGFBP 5(P < 0.009), while linearly decreasing(P < 0.001) the IGFBP 3 expression. The expression of cell apoptosis gene Caspase 8 decreased(quadratic, P = 0.012), while Bad m RNA expression tended to decrease(cubic, P = 0.053) after HG feeding.Conclusions: These results demonstrated sequential changes in rumen papillae size, cell cycle regulation and the genes involved in proliferation and apoptosis as time elapsed in feeding a high-grain diet to sheep.Lei Xu Yue Wang Junhua Liu Weiyun Zhu Shengyong Mao 2018Journal of Animal Science and Biotechnology2018,9,3:4
7Geological and surfacial processes and major disaster effects in the Yellow River Basin显示文摘The Yellow River Basin(YRB) is characterized by active geological and tectonic processes, rapid geomorphological evolution, and distinct climatic diversity. Correspondingly, major disasters in the YRB are characterized by varied types,extensive distributions, and sudden occurrences. In addition, major disasters in the YRB usually evolve into disaster chains that cause severe consequences. Therefore, major disasters in the YRB destroy ecologies and environments and influence geological and ecological safety in the basin. This paper systematically reviews research on geological and surface processes, major disaster effects, and risk mitigation in the YRB, discusses the trends and challenges of relevant research, analyzes the key scientific problems that need to be solved, and suggests prospects for future research based on the earth system science concept. Themes of research that should be focused on include geological, surface and climatic processes in the YRB and their interlinking disaster gestation mechanisms;formation mechanisms and disaster chain evolutions of giant landslides in the upper reach of the YRB;mechanisms and disaster chain effects of loess water-soil disasters in the middle reach of the YRB;occurrence patterns and amplifying effects of giant flood chains in the lower reach of the YRB;and risk mitigations of major disasters in the YRB. Key scientific problems that need to be solved are as follows: how to reveal the geological, surface and climatic processes that are coupled and interlinked with gestation mechanisms of major disasters;how to clarify the mutual feedback effects between major disasters and ecology;and how to develop a human-environmental harmony-based integrated risk mitigation system for major disasters. Prospects for future studies that follow the earth system science concept include the following: highlighting interdisciplinary research to reveal the interlinked disaster gestation mechanisms of the geology, surface and climate in the YRB in the past, present, and future;forming theories to clarify the regional patterns, dynamic mechanisms, and mutual-feedback effects between disaster chains and ecology in the YRB on land and in rivers in the region;solving technological bottlenecks to develop assessment models and mitigation theories for integrated risks in the YRB by following the human-environment harmony concept;and finally, establishing a demonstratable application pattern characterized by 'whole-basin coverage' and 'zonal controls', thereby guaranteeing ecological and geological safety in the basin and providing scientific support for ecological conservation and high-quality development of the YRB.Hengxing LAN Jianbing PENG Yanbo ZHU Langping LI Baotian PAN Qiangbing HUANG Junhua LI Qiang ZHANG 2022Science China Earth Sciences2022,65,2:4
8PI-type Iterative Learning Control for Nonlinear Electro-hydraulic Servo Vibrating System显示文摘For the electro-hydraulic servo vibrating system(ESVS) with the characteristics of non-linearity and repeating motion, a novel method, PI-type iterative learning control(ILC), is proposed on the basis of traditional PID control. By using memory ability of computer, the method keeps last time's tracking error of the system and then applies the error information to the next time's control process. At the same time, a forgetting factor and a D-type learning law of feedforward fuzzy-inferring referenced displacement error under the optimal objective are employed to enhance the systemic robustness and tracking accuracy. The results of simulation and test reveal that the algorithm has a trait of high repeating precision, and could restrain the influence of nonlinear factors like leaking, external disturbance, aerated oil, etc. Compared with traditional PID control, it could better meet the requirement of nonlinear electro-hydraulic servo vibrating system.LUO Xiaohui ZHU Yuquan HU Junhua 2009Chinese Journal of Mechanical Engineering2009,22,3:3
9Impact of Different Diets on Adult Tri-Spine Horseshoe Crab,Tachypleus tridentatus显示文摘Effective culture and management of adult tri-spine horseshoe crab,Tachypleus tridentatus can ensure that stock enhancement programs and aquaculture systems are maintained.To explore suitable feed for animals during the breeding season,Pacific oyster(Ostrea gigas)(oyster group;OG)and frozen sharpbelly fish(Hemiculter leucisculus)(frozen fish group;FG)were selected to feed 20 T.tridentatus male and female pairs,respectively.At the end of the experiment,intestinal samples were obtained to measure digestive enzymes activities.The intestinal flora were determined by 16S r DNA sequencing.No eggs were observed in the FG and one T.tridentatus adult died.No animals died in the OG,and 9.7×10;eggs were obtained.These results show that oysters are more suitable for the development and reproduction of adult T.tridentatus than frozen fish.Additionally,the digestive enzyme activity analysis revealed that animals in the OG exhibited higher protein digestibility than those in the FG,but no significant differences in lipid and carbohydrate uptake were observed between the groups.Furthermore,the intestinal flora analysis showed that operational taxonomic units(OTUs)and the Chao1 index were significantly higher in the OG than in the FG,but no significant difference was observed in the Shannon or Simpson indices between the groups.Our data indicate that the oyster diet improved the intestinal microbial diversity of T.tridentatus.We hypothesize that nutrients,such as oyster-based taurine,proteins,and highly unsaturated fatty acids,improve protease activity in the T.tridentatus digestive tract,alter the intestinal floral structure,and improve the reproductive performance of T.tridentatus.YING Ziwei BAO Yuyuan LI Yinkang YE Guoling ZHANG Shuhuan XU Peng ZHU Junhua XIE Xiaoyong 2022Journal of Ocean University of China2022,21,3:3
10Multifunctional SnO2/3D graphene hybrid materials for sodium-ion and lithium-ion batteries with excellent rate capability and long cycle life显示文摘Jung-ln Lee Junhua Song Younghwan Cha Shaofang Fu Chengzhou Zhu Xiaolin Li Yuehe Lin Min-Kyu Song 2017Nano Research2017,10,12:2
11The effect of nozzle layout on droplet ejection of a piezo-electrically actuated micro-atomizer显示文摘我们这里在一个微雾化器的微滴喷射上学习嘴布局的效果,它被 MEMS 技术与穿的嘴制作并且由一个压电的磁盘激活。一个理论模型首先为联合系统在液体房间描绘声学的波浪繁殖的这 piezoelectric-liquid-structure 被造,它从嘴决定了微滴形成。形式的分析数字地被执行预言反响的频率并且模仿对应压力波浪领域。由比较压力的轮廓与设计的嘴布局在嘴入口在液体固体接口上挥动的振幅,在不同颤动模式下面的设备的行为能被预言。试验性地,一个阻抗分析器被用来测量系统的反响的频率。有不同的嘴布局的 atomizers 的三种类型为在喷射表演上测量嘴分发的效果被制作。微滴产生的可视化实验被执行,这些设备的体积流动率被测量。在实验和预言之间的好协议证明仅仅嘴的增加不能提高微滴产生,从频率的一个观点的嘴分发的一个图案为反响的相关 atomizer 是必要的。Yanying Feng Zhaoying Zhou Junhua Zhu Guibin Du 2007Acta Mechanica Sinica2007,23,2:2
12Using trace elements of magnetite to constrain the origin of the Pingchuan hydrothermal low-Ti magnetite deposit in the Panxi area, SW China显示文摘The Pingchuan iron deposit, located in the Yanyuan region of Sichuan Province, SW China, has an ore reserve of 40 Mt with ~60 wt% Fe. Its genesis is still poorly understood. The Pingchuan iron deposit has a paragenetic sequence of an early Fe-oxide–Pyrite stage(Ⅰ) and a late Fe-oxide–pyrrhotite stage(Ⅱ). Stage Ⅰ magnetite grains are generally fragmented, euhedral–subhedral, largesized crystals accompanying with slightly postdated pyrite.Stage Ⅱ magnetite grains are mostly unfragmented, anhedral, relatively small-sized grains that co-exist with pyrrhotite. Combined with micro-textural features and previously-obtained geochronological data, we consider that these two stages of iron mineralization in the Pingchuan deposit correspond to the Permian ELIP magmatism and Cenozoic fault activity event. Both the Stage Ⅰ and Ⅱ magnetites are characterized with overall lower contents of trace elements(including Cr, Ti, V, and Ni) than the ELIP magmatic magnetite, which suggests a hydrothermal origin for them. ‘‘Skarn-like'' enrichment in Sn, Mn, and Zn in the Stage Ⅰ magnetite grains indicate significant material contributions from carbonate wall-rocks due to water–rock interaction in ore-forming processes. Stage Ⅱ magnetite grains contain higher Mn concentrations than Stage Ⅰ magnetite grains, which possibly implies more contribution from carbonate rocks. In multiple-element diagrams, the Stage Ⅰ magnetite shows systematic similarities to Kiruna-type magnetite rather than those from other types of deposits. Combined with geological features and previous studies on oxygen isotopes, we conclude that hydrothermal fluids have played a key role in the generation of the Pingchuan low-Ti iron deposit.Yanjun Wang Weiguang Zhu Hong Zhong Zhongjie Bai Junhua Yao Chong Xu 2019Acta Geochimica2019,38,3:2
13Spatial variation and driving mechanism of soil organic carbon components in the alluvial/sedimentary zone of the Yellow River显示文摘Alluviation and sedimentation of the Yellow River are important factors influencing the surface soil structure and organic carbon content in its lower reaches.Selecting Kaifeng and Zhoukou as typical cases of the Yellow River flooding area,the field survey,soil sample collection,laboratory experiment and Geographic Information System(GIS)spatial analysis methods were applied to study the spatial distribution characteristics and change mechanism of organic carbon components at different soil depths.The results revealed that the soil total organic carbon(TOC),active organic carbon(AOC)and nonactive organic carbon(NOC)contents ranged from 0.05–30.03 g/kg,0.01–8.86 g/kg and 0.02–23.36 g/kg,respectively.The TOC,AOC and NOC contents in the surface soil layer were obviously higher than those in the lower soil layer,and the sequence of the content and change range within a single layer was TOC>NOC>AOC.Geostatistical analysis indicated that the TOC,AOC and NOC contents were commonly influenced by structural and random factors,and the influence magnitudes of these two factors were similar.The overall spatial trends of TOC,AOC and NOC remained relatively consistent from the 0–20 cm layer to the 20–100 cm layer,and the transition between high-and low-value areas was obvious,while the spatial variance was high.The AOC and NOC contents and spatial distribution better reflected TOC spatial variation and carbon accumulation areas.The distribution and depth of the sediment,agricultural land-use type,cropping system,fertilization method,tillage process and cultivation history were the main factors impacting the spatial variation in the soil organic carbon(SOC)components.Therefore,increasing the organic matter content,straw return,applying organic manure,adding exogenous particulate matter and conservation tillage are effective measures to improve the soil quality and attain sustainable agricultural development in the alluvial/sedimentary zone of the Yellow River.LI Guodong ZHANG Junhua ZHU Lianqi TIAN Huiwen SHI Jiaqi REN Xiaojuan 2021Journal of Geographical Sciences2021,31,4:2
14Transcriptomic analysis reveals the molecular mechanisms of rumen wall morphological and functional development induced by different solid diet introduction in a lamb model显示文摘Background:This study aimed to elucidate the molecular mechanisms of solid diet introduction initiating the cellular growth and maturation of rumen tissues and characterize the shared and unique biological processes upon different solid diet regimes.Methods:Twenty-four Hu lambs were randomly allocated to three groups fed following diets:goat milk powder only(M,n=8),goat milk powder+alfalfa hay(MH,n=8),and goat milk powder+concentrate starter(MC,n=8).At 42 days of age,the lambs were slaughtered.Ruminal fluid sample was collected for analysis of concentration of volatile fatty acid(VFA)and microbial crude protein(MCP).The sample of the rumen wall from the ventral sac was collected for analysis of rumen papilla morphology and transcriptomics.Results:Compared with the M group,MH and MC group had a higher concentration of VFA,MCP,rumen weight,and rumen papilla area.The transcriptomic results of rumen wall showed that there were 312 shared differentially expressed genes(DEGs)between in“MH vs.M”and“MC vs.M”,and 232 or 796 unique DEGs observed in“MH vs.M”or“MC vs.M”,respectively.The shared DEGs were most enriched in VFA absorption and metabolism,such as peroxisome proliferator-activated receptor(PPAR)signaling pathway,butanoate metabolism,and synthesis and degradation of ketone bodies.Additionally,a weighted gene co-expression network analysis identified M16(2,052 genes)and M18(579 genes)modules were positively correlated with VFA and rumen wall morphology.The M16 module was mainly related to metabolism pathway,while the M18 module was mainly associated with signaling transport.Moreover,hay specifically depressed expression of genes involved in cytokine production,immune response,and immunocyte activation,and concentrate starter mainly altered nutrient transport and metabolism,especially ion transport,amino acid,and fatty acid metabolism.Conclusions:The energy production during VFA metabolism may drive the rumen wall development directly.The hay introduction facilitated establishment of immune function,while the concentrate starter enhanced nutrient transport and metabolism,which are important biological processes required for rumen development.Daming Sun Yuyang Yin Changzheng Guo Lixiang Liu Shengyong Mao Weiyun Zhu Junhua Liu 2021Journal of Animal Science and Biotechnology2021,12,3:2
15Ontogenetic resource use and trophic dynamics of endangered juvenile Tachypleus tridentatus among diversified nursery habitats in the northern Beibu Gulf,China显示文摘Horseshoe crabs,the most well-known example of“living fossils”,are iconic and ecologically important macroinvertebrates in coastal and estuarine ecosystems.Their blood is a crucial resource for manufacturing Limulus or Tachypleus amebocyte lysate to detect bacterial endotoxins or fungal contamination in drug and medical devices.An enhanced understanding of their ecological roles and trophic interactions in the food webs is fundamental to facilitate resource management for the declining populations in Asia.Foraging information of the Asian species,however,is mainly derived from preliminary,scattered reports from a limited number of study locations.In this study,resource utilization,trophic niche dynamics,and trophic interaction of the juvenile tri-spine horseshoe crab,Tachypleus tridentatus(instars 1–12,approximately 0.5–8 years old)across ontogeny was assessed in diversified nursery habitats along the northern Beibu Gulf,China,using carbon and nitrogen stable isotopes.Our results suggest that:(i)T.tridentatus are ecological generalists given the vast range of carbon isotopic values and trophic niche width estimates exhibited between multiple instar groups;(ii)juvenile T.tridentatus across most habitat types predominantly assimilated energy from a variety of basal production sources in the food web,but primarily depended on sedimentary organic matter and seagrass resource pools;(iii)ontogenetic shifts in juvenile dietary proportions were evident,with decreased reliance on sedimentary organic matter,coupled with increased reliance on benthic macroinvertebrate grazers,detritivores,and omnivores with age;and(iv)nearly all juvenile instars occupied similar trophic positions in the food web with slight shifts in trophic position present with increasing size.Our findings indicate that resource availability and ontogenetic diet shifts strongly influence horseshoe crab trophic dynamics,and age should be accounted when formulating habitat conservation measures based on resource use for Asian horseshoe crabs.Kit Yue KWAN Justin BOPP Shuyan HUANG Qiao CHEN Chun-Chieh WANG Xueping WANG Wenquan ZHEN Junhua ZHU Xing HUANG 2021Integrative Zoology2021,16,6:1
16Template-directed synthesis of nitrogen- and sulfur- codoped carbon nanowire aerogels with enhanced electrocatalytic performance for oxygen reduction显示文摘做的 Heteroatom,精确作文控制,和合理形态学设计是为在燃料房间为氧减小反应(ORR ) 生产新奇 nanocatalysts 的有效策略。此处,综合的一条划算的途径用一个难 templating 方法的 nitrogen-codoped 和 sulfur-codoped 碳 nanowire aerogels 被建议。aerogels 在碱的答案为 ORR 用热水的处理和碳化展览的联合准备了好催化活动。在在氮和硫先锋之间的最佳的退火温度和集体比率,结果的 aerogels 表演可比较的 electrocatalytic 活动到为 ORR 的商业 Pt/C 催化剂的。重要地,优化催化剂为甲醇转线路效果显示出更好长期的稳定性和令人满意的忍耐。这些 codoped aerogels 被期望在燃料房间有潜在的应用。Shaofang Fu Chengzhou Zhu Junhua Song Mark H. Engelhard Xiaolin Li Peina Zhang Haibing Xia Dan Du Yuehe Lin 2017Nano Research2017,10,6:1
17Implementing wavelet transform with SAW elements 显示文摘Lu Wenke Zhu Changchun Liu Junhua 2003Science in China (Series E)2003,46,6:1
18Mining consistent fraud behavior in hospz- talization 显示文摘Junhua He Y Xiong Y Y Zhu 2011Computer Applications and Software2011,28,7:1
19Study of Improving Identification Accuracy of Carbon Nanotube Film Cathode Gas Sensor 显示文摘Zhang Yong Liu Junhua Li Xin Tang Xiaojun Zhu Changchun 2005Sensors and Actuators B2005,125,1:1
20Shale gas reservoir characterisation: A typical case in the southern Sichuan Basin of China显示文摘Shangbin Chen Yanming Zhu Hongyan Wang Honglin Liu Wei Wei Junhua Fang 2011Energy2011,,11:1
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