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1Heterologous biosynthesis of natural product naringenin by co-culture engineering显示文摘Co-culture engineering is an emerging approach for microbial biosynthesis of a variety of biochemicals.In this study,E.coli-E.coli co-cultures were developed for heterologous biosynthesis of the natural product naringenin.The co-cultures were composed of two independent E.coli strains dedicated to functional expression of different portions of the biosynthetic pathway,respectively.The co-culture biosynthesis was optimized by investigating the effect of carbon source,E.coli strain selection,timing of IPTG induction and the inoculation ratio between the co-culture strains.Compared with the monoculture strategy,the utilization of the designed co-cultures significantly improved the naringenin production,largely due to the reduction of metabolic stress,employment of proper hosts for improving pathway enzyme activities,and flexible adjustment of the relative biosynthetic strength between the coculture strains.The findings of this study extend the applicability of co-culture engineering in complex natural product biosynthesis.Vijaydev Ganesan Zhenghong Li Xiaonan Wang Haoran Zhang 2017Synthetic and Systems Biotechnology2017,2,3:13
2Formation and timing of the extensional ductile shear zones in Yiwulü Mountain area,Western Liaoning Province,North China显示文摘The NNE-trending ductile shear zones in Yiwulü Mountain area were formed in relation to two successive extensional events.The Yiwulü High Temperature Extensional Ductile Shear Zone and the Waziyu Low Temperature Extensional Ductile Shear Zone were related to ductile deformation at higher temperatures and brittle-ductile deformation at lower temperatures,respectively.Both deformations were accompanied by large scale volcanic eruptions and magmatic intrusions.Based on structural analysis of macroscopic and microscopic deformations,and quartz lattice preferred orientations,we show that the early Yiwulü High Temperature Extensional Ductile Shear Zone was resulted from a NE-SW extension at amphibolite facies in the middle crust,whereas the Waziyu Low Temperature Extensional Ductile Shear Zone was due mainly to a NWW-SEE extension at greenschist facies in the upper crust.The SHRIMP zircon age of a syn-tectonic granitic dike emplaced at the late stage of high temperature extension is 155±2 Ma,indicating that the early extensional event took place in the Middle-Late Jurassic.40Ar-39Ar age of muscovite from tectonic schists in the low temperature extensional ductile shear zone is 131.6±1.0 Ma,suggesting that the late extension occurred in the Early Cretaceous.Subsequent overall uplifting succeeded the late extension.The new discovery of the Middle-Late Jurassic NNE-trending extensional ductile shear zone provides evidence constraining the switch of tectonic regimes and Middle Jurassic thinning of lithosphere in the eastern North China Craton.LI Gang LIU ZhengHong LIU JunLai LI YongFei XU ZhongYuan DONG XiaoJie 2012Science China Earth Sciences2012,55,5:11
3Aerodynamic design optimization of nacelle/pylon position on an aircraft显示文摘The arbitrary space-shape free form deformation (FFD) method developed in this paper is based on non-uniform rational B-splines (NURBS) basis function and used for the integral parameterization of nacelle-pylon geometry. The multi-block structured grid deformation technique is established by Delaunay graph mapping method. The optimization objects of aerodynamic characteristics are evaluated by solving NavierStokes equations on the basis of multi-block structured grid. The advanced particle swarm optimization (PSO) is utilized as search algorithm, which com-bines the Kriging model as surrogate model during optimization. The optimization system is used for optimizing the nacelle location of DLR-F6 wing-body-pylon-nacelle. The results indicate that the aerodynamic interference between the parts is significantly reduced. The optimization design system established in this paper has extensive applications and engineering value.Li Jing Gao Zhenghong Huang Jiangtao Zhao Ke 2013Chinese Journal of Aeronautics2013,26,4:10
4A two-step lineage reprogramming strategy to generate functionally competent human hepatocytes from fibroblasts显示文摘Terminally differentiated cells can be generated by lineage reprogramming,which is,however,hindered by incomplete conversion with residual initial cell identity and partial functionality. Here, we demonstrate a new reprogramming strategy by mimicking the natural regeneration route, which permits generating expandable hepatic progenitor cells and functionally competent human hepatocytes. Fibroblasts were first induced into human hepatic progenitor-like cells (hHPLCs), which could robustly expand in vitro and efficiently engraft in vivo. Moreover, hHPLCs could be efficiently induced into mature human hepatocytes (hiHeps) in vitro, whose molecular identity highly resembles primary human hepatocytes (PHHs). Most importantly, hiHeps could be generated in large quantity and were functionally competent to replace PHHs for drug-metabolism estimation, toxicity prediction and hepatitis B virus infection modeling. Our results highlight the advantages of the progenitor stage for successful lineage reprogramming. This strategy is promising for generating other mature human cell types by lineage reprogramming.Bingqing Xie Da Sun Yuanyuan Du Jun Jia Shicheng Sun Jun Xu Yifang Liu Chengang Xiang Sitong Chen Huangfan Xie Qiming Wang Guangya Li Xuehui LYU Hui Shen Shiyu Li Min Wu Xiaonan Zhang Yue Pu Kuanhui Xiang Weifeng Lai Peng Du Zhenghong Yuan Cheng Li Yan Shi Shichun Lu Hongkui Deng 2019Cell Research2019,29,9:9
5Robust design of NLF airfoils显示文摘A robust optimization design approach of natural laminar airfoils is developed in this paper. First, the non-uniform rational B-splines (NURBS) free form deformation method based on NURBS basis function is introduced to the airfoil parameterization. Second, aerodynamic characteristics are evaluated by solving Navier-Stokes equations, and theγ-Reθt transition model coupling with shear-stress transport (SST) turbulent model is introduced to simulate boundary layer transition. A numerical simulation of transition flow around NLF0416 airfoil is conducted to test the code. The comparison between numerical simulation results and wind tunnel test data approves the validity and applicability of the present transition model. Third, the optimization system is set up, which uses the separated particle swarm optimization (SPSO) as search algorithm and combines the Kriging models as surrogate model during optimization. The system is applied to carry out robust design about the uncertainty of lift coefficient and Mach number for NASA NLF-0115 airfoil. The data of optimized airfoil aerodynamic characteristics indicates that the optimized airfoil can maintain laminar flow stably in an uncertain range and has a wider range of low drag.Li Jing Gao Zhenghong Huang Jiangtao Zhao Ke 2013Chinese Journal of Aeronautics2013,26,2:9
6Aerodynamic optimization of rotor airfoil based on multi-layer hierarchical constraint method显示文摘Rotor airfoil design is investigated in this paper. There are many difficulties for this highdimensional multi-objective problem when traditional multi-objective optimization methods are used. Therefore, a multi-layer hierarchical constraint method is proposed by coupling principal component analysis(PCA) dimensionality reduction and e-constraint method to translate the original high-dimensional problem into a bi-objective problem. This paper selects the main design objectives by conducting PCA to the preliminary solution of original problem with consideration of the priority of design objectives. According to the e-constraint method, the design model is established by treating the two top-ranking design goals as objective and others as variable constraints. A series of bi-objective Pareto curves will be obtained by changing the variable constraints, and the favorable solution can be obtained by analyzing Pareto curve spectrum. This method is applied to the rotor airfoil design and makes great improvement in aerodynamic performance. It is shown that the method is convenient and efficient, beyond which, it facilitates decision-making of the highdimensional multi-objective engineering problem.Zhao Ke Gao Zhenghong Huang Jiangtao Li Quan 2016Chinese Journal of Aeronautics2016,29,6:7
7New insights into hepatitis B virus biology and implications for novel antiviral strategies显示文摘Hepatitis B virus(HBV), a small DNA virus with a unique replication mode, can cause chronic hepatitis(CHB), which is characterized by the persistence of the viral covalently closed circular DNA that serves as the template for HBV replication and the production of large amounts of secreted HBV surface antigen(HBs Ag) that is present in excess of the levels of infectious virus. Despite the success of currently approved antiviral treatments for CHB patients, including interferon and nucleotide analogs, which suppress HBV replication and reduce the risk of CHB-related liver diseases, these therapies fail to eradicate the virus in most of the patients. With the development of the cell and animal models for HBV study, a beter understanding of the HBV life cycle has been achieved and a series of novel antiviral strategies that target diferent stages of HBV replication have been designed to overcome the viral factors that contribute to HBV persistence. Such basic HBV research advancements and therapeutic developments are the subject of this review.Jieliang Chen Min Wu Kuancheng Liu Wen Zhang Yaming Li Xiaohui Zhou Lu Bai Zhenghong Yuan 2015National Science Review2015,2,3:6
8A Salmonella enterica serovar Typhi plasmid induces rapid and massive apoptosis in infected macrophages显示文摘pRST98 is a chimeric plasmid isolated from Salmonella enterica serovar Typhi(S.typhi)that mediates the functions of drug resistance and virulence.Previously,we reported that Salmonella plasmid virulence(spv)genes were present in S.typhi.In our current study,we investigated whether plasmid pRST98 exhibits significant cytotoxicity in macrophages.pRST98 was transferred into the avirulent Salmonella enterica serovar Typhimurium(S.typhimurium)strain RIA to create the transconjugant pRST98/RIA.The standard S.typhimurium virulent strain SR-11,which carries a 100-kb virulence plasmid,was used as a positive control.The bacterial strains were incubated with a murine macrophage-like cell line(J774A.1)in vitro.Apoptosis of J774A.1 cells was examined by electron microscopy and flow cytometry after annexin-V/propidium iodide labeling,and the survival of Salmonella strains in J774A.1 cells was determined.Results showed that macrophages infected with strain pRST98/RIA displayed greater levels of apoptosis than those infected with RIA and that pRST98 may increase bacterial survival in macrophages.Further studies showed that the pRST98-induced death of macrophages was associated with the loss of mitochondrial membrane potential and that pRST98 may activate caspase-9 and then caspase-3.The research data indicate that the virulence of bacteria that contain the pRST98 plasmid is enhanced;the presence of this plasmid increases the survival of the bacterial pathogen and acts through the mitochondrial pathway to mediate macrophage apoptosis.Shuyan Wu Yuanyuan Li Yang Xu Qiong Li Yuanyuan Chu Rui Huang Zhenghong Qin 2010Cellular & Molecular Immunology2010,7,4:6
9Identifying changes in the wheat kernel proteome under heat stress using iTRAQ显示文摘Wheat(Triticum aestivum L.) is one of the three major global food crops. Hightemperature stress can affect its yield and quality. Studies of the effect of hightemperature stress on wheat kernel development are important because they can reveal the stability of wheat quality and lead to the genetic improvement of wheat quality traits. In this study, the isobaric tags for relative and absolute quantitation(iTRAQ)method was adopted to analyze changes in the protein expression profile of wheat cultivars under high temperature stress. The protein content of wheat grain increased under heat stress, while the SDS-sedimentation value and starch content decreased.Grain filling was deficient under high temperature stress, which reduced thousandkernel weight but did not affect wheat kernel length. The 207 differentially expressed proteins identified in Gaocheng 8901 under heat stress were associated with energy metabolism, growth and development, and stress response. Gene Ontology enrichment analysis showed that the annotated proteins that were differentially expressed in Gaocheng 8901 under heat stress were involved mainly in stimulus response, abiotic stress response, stress response, and plasma membrane. A set of 78 differentially expressed proteins were assigned to 83 KEGG signaling/metabolic pathways. KEGG pathway enrichment analysis showed that this set of proteins was significantly enriched in members of 51 pathways, and the proteins participated mainly in protein synthesis in the endoplasmic reticulum, starch and sucrose metabolism, and reaction on ribosomes. Five differentially expressed proteins were involved in protein–protein interaction networks that may greatly influence the yield and quality of wheat grain. In wheat, high-temperature stress leads to a variety of effects on protein expression and may ultimately cause changes in yield and quality.Yufeng Zhang Hongyao Lou Dandan Guo Ruiqi Zhang Meng Su Zhenghong Hou Haiying Zhou Rongqi Liang Chaojie Xie Mingshan You Baoyun Li 2018The Crop Journal2018,6,6:5
10Characteristics of highly differentiated granite and metallization of tungsten-tin, rare and rare earth metal in the eastern Nanling region, China显示文摘1 Introduction Massive tungsten-tin,rare and rare earth metals ore deposits were formed with the widespread granite magmatic activity in early Yanshanian period in the eastern Nanling region.Recent studies indicate that the Yanshanian highly differentiated-granite formation is closely related to the deposits of tungsten and tin,rare and rare earth metals mineralization in theXIAO Huiliang CHEN Lezhu FAN Feipeng LI Haili BAO Xiaoming YAO Zhenghong ZHOU Yan CAI Yitao 2017Acta Geologica Sinica(English Edition)2017,91,S1:5
11Ductile and ultrahigh-strength eutectic high-entropy alloys by large-volume 3D printing显示文摘1.Introduction Additive manufacturing or 3D printing outweighs conventional casting methods in the aspect of complex parts fabrication,which can realize one-step formation without the need of complicated cast dies.3D printing significantly promotes industrial production for making near-net shaped components.However,this promising technique is not always ideally applicable for metals and alloys.For example,titanium alloys prepared by 3D printing often suf-fer from poor plasticity,and usually require further complex heat treatment or hot isostatic pressing treatment,in order to remove internal stress and regulate plasticity and strength[1,2],which de-feats the original intention of employing additive manufacturing.One of the fundamental causes for such issues is the low fluidity of the alloys upon melting,leading to great chemical heterogeneity,high porosity content and residual stresses.This limitation hinders further design and fabrication of high-performance printable alloys from large scale production and application.Yiping Lu Xiaoxiang Wu Zhenghong Fu Qiankun Yang Yong Zhang Qiming Liu Tianxin Li Yanzhong Tian Hua Tan Zhiming Li Tongmin Wang Tingju Li 2022Journal of Materials Science & Technology2022,,31:4
12Bioinformatic analysis of expressed sequence tags from sporophyte of Porphyra yezoensis (Bagiaceae, Rhodophyta)显示文摘719 个表示顺序标签(EST ) 的一个总数非聚类进 329 - 冗余的 EST 组从红 alage 的 sporophte cDNA 图书馆被获得, Porphyra yezoensis。基因本体论(去) 分析在描绘 60 最严格的 annotatd 被采用从象与在房间工作的酶和蛋白质有关的 COXI, Sod_Fe_C, GST_N, SHMT,和 RNase_PH 那样的 329 个 EST 组和一些领域的唯一的基因被 HMMPFAM 搜索识别了。作为它的叶状的配偶体,有 storong 偏爱的用法上的类似的鳕鱼在 P 被发现。yezoensis 细丝状的孢子体,不管在给定的氨基酸发现的一些差别。329 唯一的基因的平均 GC 内容是 53.0 % 。相反,鳕鱼在上的第三核苷酸比的展出一个更高的 GC 内容(72 %) 第一(58 %) 并且第二(42 %) 核苷酸。现在的学习的类似搜索显示出对 Porphyra EST 数据库的 60.2 % 的 noves EST 比率,建议向阐明的进一步的调查 Porphyrafunctional 的特征染色体。XU Minjun MAO Yunxiang ZHANG Xuecheng ZHOU Xiaojun SUI Zhenghong ZHOU Hailin LI Jinhong 2006Progress in Natural Science:Materials International2006,16,1:4
13Detrital Zircon U–Pb Geochronology and Provenance of Bayan Obo Group, Northern Margin of North China Craton: New Implications for the Position of NCC in Rodinia显示文摘The paleoposition of North China Craton in Rodinia has long been in controversial. This paper mainly focuses on the U-Pb geochronological studies of detrital zircons obtained from Bayan Obo Group exposed in the Shangdu area, Inner Mongolia, aiming to provide more information for interprating this problem. Based on the acquired data, this paper comes to the following conclusions. Firstly, the depositional age of Bayan Obo Group might be from Meso- to Neoproterozoic according to the zircons U-Pb dating results. The lower succession of this group, namely Dulahala and Jianshan formations deposited between 1800 and 1650 Ma. The Halahuogete and Bilute formations deposited between 1500 and 1350 Ma. For Baiyinbaolage and Hujiertu formations, their depositional age was 1250-900 Ma. Secondly, for the provenance of Bayan Obo Group, this paper believes detrital zircons with age of 2.51-2.71 Ga and 2.00-2.48 Ga were from Guyang, Xi Ulanbulang and Zhuozi area;the Khondalite Belt provided detrital zircons with age of 1.95-1.80 Ga;zircons with age of 1.60-1.75 Ga might come from granitic rocks in Miyun Area. The magmatism after 1.60 Ga was rarely recorded in the NCC, therefore those zircons with ages younger than 1.60 Ga might come from outside of NCC. The magmatism with the same age existed in Baltic, Amazonia and Laurentia. Based on previous paleomagnetic researches, this paper proposes that NCC might receive detritus from Baltic during 1560-1350 Ma and had affinity with Laurentia and Amazonia at ~0.9 Ga in Rodinia. Baltic, Amazonia and Laurentia might be potential provenances for non-NCC detritus in Bayan Obo Group.LI Changhai LIU Zhenghong XU Zhongyuan DONG Xiaojie LI Pengchuan SHI Qiang WANG Shijie 2019Acta Geologica Sinica(English Edition)2019,93,5:4
14Confined Ni-In intermetallic alloy nanocatalyst with excellent coking resistance for methane dry reforming显示文摘Carbon dioxide and methane are two main greenhouse gases which are contributed to serious global warming.Fortunately,dry reforming of methane(DRM),a very important reaction developed decades ago,can convert these two major greenhouse gases into value-added syngas or hydrogen.The main problem retarding its industrialization is the seriously coking formation upon the nickel-based catalysts.Herein,a series of confined indium-nickel(In-Ni)intermetallic alloy nanocatalysts(In_(x)Ni@SiO_(2))have been prepared and displayed superior coking resistance for DRM reaction.The sample containing 0.5 wt.%of In loading(In_(0.5)Ni@SiO_(2))shows the best balance of carbon deposition resistance and DRM reactivity even after 430 h long term stability test.The boosted carbon resistance can be ascribed to the confinement of core–shell structure and to the transfer of electrons from Indium to Nickel in In-Ni intermetallic alloys due to the smaller electronegativity of In.Both the silica shell and the increase of electron cloud density on metallic Ni can weaken the ability of Ni to activate C–H bond and decrease the deep cracking process of methane.The reaction over the confined InNi intermetallic alloy nanocatalyst was conformed to the Langmuir-Hinshelwood(L-H)mechanism revealed by in situ diffuse reflectance infrared Fourier transform spectroscopy(in-situ DRIFTS).This work provides a guidance to design high performance coking resistance catalysts for methane dry reforming to efficiently utilize these two main greenhouse gases.Wenming Liu Le Li Sixue Lin Yiwei Luo Zhenghong Bao Yiru Mao Kongzhai Li Daishe Wu Honggen Peng 2022Journal of Energy Chemistry2022,31,2:3
15Recent Advances in Super-Resolution Fluorescence Imaging and Its Applications in Biology显示文摘Fluorescence microscopy has become an essential tool for biological research because it can be minimally invasive, acquire data rapidly, and target molecules of interest with specific labeling strategies. However, the diffraction-limited spatial resolution, which is classically limited to about 200 nm in the lateral direction and about 500 nm in the axial direction, hampers its application to identify delicate details of subcellular structure. Extensive efforts have been made to break diffraction limit for obtaining high-resolution imaging of a biological specimen. Various methods capable of obtaining super-resolution images with a resolution of tens of nanometers are currently available. These super-resolution techniques can be generally divided into three primary classes:(1) patterned illuminationbased super-resolution imaging, which employs spatially and temporally modulated illumination light to reconstruct sub-diffraction structures;(2) single-molecule localization-based super-resolution imaging, which localizes the profile center of each individual fluorophore at subdiffraction precision;(3) bleaching/blinking-based super-resolution imaging. These super-resolution techniques have been utilized in different biological fields and provide novel insights into several new aspects of life science. Given unique technical merits and commercial availability of super-resolution fluorescence microscope, increasing applications of this powerful technique in life science can be expected.Rongcheng Han Zhenghong Li Yanyan Fan Yuqiang Jiang 2013Journal of Genetics and Genomics2013,40,12:3
16A Surfactant-stable Bacillus pumilus K9 α-Keratinase and Its Potential Application in Detergent Industry显示文摘GONG Jinsong WANG Yue ZHANG Dandan LI Heng ZHANG Xiaomei ZHANG Rongxian LU Zhenming XU Zhenghong SHI Jinsong 2015Chemical Research in Chinese Universities2015,31,1:3
17New Discovery of the Late Triassic Terrigenous Sediments in the Great Xing''an Range Region,NE China and its Geological Significance显示文摘Objective The Great Xing'an Range is located in the eastern section of Central Asian Orogenic Belt(CAOB).As a superposed position of multiple tectonic domains,its structural evoIlution has always been a focused issue of geological research.The Triassic is a critical turningLI Shichao ZHANG Lingyu LIU Zhenghong XU Zhongyuan 2017Acta Geologica Sinica(English Edition)2017,91,5:2
18Characteristics of array of distributed synthetic jets and effect on turbulent boundary layer显示文摘An array of distributed round synthetic jets was used to control a fully developed turbulent boundary layer.The study focused on the related skin friction drag reduction and mechanisms involved.The control effects were analyzed by measuring the streamwise velocities using a hot-wire anemometer downstream of the array.A reduction in the skin friction was observed both in the regions downstream of the orifices and in the regions between two adjacent orifices.A statistical analysis with the variable-interval time-averaging(VITA)technique demonstrated a weakened bursting intensity with synthetic jet in the near-wall region.The streamwise vortices were lifted by the upwash effect caused by synthetic jet and induced less low-speed streaks.The control mechanism acted in a way to suppress the dynamic interaction between the streamwise vortices and low-speed streaks and to attenuate the turbulence production in the near-wall region.The forcing frequency was found to be a more relevant parameter when synthetic jet was applied in turbulent boundary layer flow control.A higher forcing frequency induced a higher reduction in the skin friction.The power spectral density and autocorrelation of the fluctuating velocities showed that the synthetic jets gradually decayed in the streamwise direction,having an effect as far as 34.5 times the displacement thickness that was on the trailing edge of the distributed synthetic jets array.Lianshan Lu Dong Li Zhenghong Gao Zhen Cao Yu Bai Jie Zheng 2020Acta Mechanica Sinica2020,36,6:2
19Hybrid photoanode films based on sparse ZnO rod array-TiO_2 nanoparticles in dye-sensitized solar cells显示文摘ZnO-TiO2 hybrid photoanodes were fabricated via the doctor-blade method by integrating vertically-grown sparse ZnO arrays with hydrothermal TiO2 nanoparticles. A special surface-coating technique was developed to deposit a thin TiO2 layer on the surface of ZnO rods. Microstructure, optical and photoelectrochemical performance of the hybrid photoanodes were investigated. The denser ZnO array exhibited bad filling behavior of nanoparticles in the interspace of ZnO rods, strong scattering and low conversion efficiency (0.27%). The sparser array showed a much better integrated microstructure, improved transmittance and high conversion efficiency (2.68%). The surface modification of ZnO rods by the TiO2 thin layer was found useful in improving the interfacial microstructure between the ZnO rod and the TiO2 bulk film, and the total conversion efficiency of 3.01% was achieved, higher than that of the pure TiO2 nanoparticle cell (2.93%). The increased scattering effects on the incident light, the enhanced electron transportation at TiO2/dye/electrolyte interface, and the inhabited recombination were responsible for this improvement.WANG CaiLu GAO XiangDong LI XiaoMin JIANG ZhengWu YANG ZhengHong GU ZhengYing HE Peng 2012Science China(Physics,Mechanics & Astronomy)2012,55,7:2
20New Discovery of ~2.65 Ga Tonalitic Gneiss in the Southern Jilin Province, China显示文摘Objective The North China Craton(NCC)is almost continuously widespread TTGs of 3.8–2.5 Ga,and the intrusive peak appears at^2.53 Ga,indicating that the Neoarchean is the most important period of continental growth in the NCC(Wan Yusheng et al.,2017).Recently studies reveal that the TTGs were formed during^2.7 Ga and 2.6–2.5 Ga in southern Jilin Province,NE of the NCC(Wang Chaoyang et al.,2017;Guo et al.,2017),whereas 2.7–2.6LI Pengchuan LIU Zhenghong XU Zhongyuan DONG Xiaojie LI Shichao 2018Acta Geologica Sinica(English Edition)2018,92,5:2
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