|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | The RNA Editing Factor WSP1 Is Essentia for Chloroplast Development in Rice显示文摘尽管在 Arabidopsis thaliana 的质体编辑因素(MORF ) 的多重 organellar RNA 是位于下面 MORF 怎么在另外的种类影响植物开发的广泛地学习的、分子的细节,特别地在米饭,仍然保持大部分未知。这里,我们描述 wsp1 的描述,有白条纹的叶子和圆锥花序的米饭异种。尤其是, wsp1 在出发舞台展出了将近白的不成熟的圆锥花序。传播电子显微镜学分析和叶绿素内容测量揭示了叶绿体在 wsp1 的发展缺点和减少的叶绿素累积。在 Os04g51280 发现一个点变化的 WSP1 的位置的克隆,份额其通常认为的产品高与 MORF 蛋白质定序类似。互补实验证明 WSP1 为 wsp1 的使成杂色的显型负责。WSP1 对叶绿体局部性,在 wsp1 的点变化影响编辑多重 organellar RNA 地点。由于在编辑的质体 RNA 的缺点,叶绿体核糖体生物的续生说并且 ndhA 拼接也在 wsp1 被损害,它可以在出发阶段在叶子和圆锥花序影响正常叶绿体开发。一起,我们的结果在叶绿体开发表明米饭 WSP1 蛋白质的重要性并且在米饭关于 MORF 家庭成员拓宽我们的知识。 | Zhiguo Zhang Xuean Cui Yanwei Wang JinxiaWu Xiaofeng Gu Tiegang Lu | 2017 | Molecular Plant2017，10，1： | 15 |
| 2 | Acetyl salicylic acid attenuates cardiac hypertrophy through Wnt signaling显示文摘室的肥大是心血管的疾病的事件的一个强大、独立的预言者。低剂量的乙酰水杨酸酸(阿司匹林) 的脉管的性质通过血小板 cyclooxygenase 的不可逆的抑制提供心血管的好处 1;然而,可能的 anti-hypertrophic 性质和阿司匹林的潜在的机制没详细被调查。在这研究,健康野类型的雄的老鼠随机被划分成三个组并且使遭到了到横向的大动脉的收缩(TAC ) 或假冒的操作。操作 TAC 的老鼠与低剂量的阿司匹林的人的等价物被对待(10 mg 吗? | Samuel Chege Gitau Xuelian Li Dandan Zhao Zhenfeng Guo Haihai Liang Ming Qian Lifang Lv Tianshi Li Bozhi Xu Zhiguo Wang Yong Zhang Chaoqian Xu Yanjie Lu Zhiming Du Hongli Shan Baofeng Yang | 2015 | Frontiers of Medicine2015，9，4： | 6 |
| 3 | The effect of 2,4-diamino-6-hydroxy-pyrimidine on PoStburn Staphylococcus aureus sepsis in rats显示文摘Objective;GTP-cyclohydrolase I (GTP-CHI) is the first and rate-limiting enzyme for the de novo bio-synthesis of biopterin.The present study was to observe the effect of 2,4-diamino-6-hydroxy-pyrimidine (DAHP),an inbibtor of GTP-CHI,on the development of postburn Staphylococcus aureus sepsis.Methods;56 male Wistarrats were randomly divided into four groups as follows;normal control group (n=10),scald control group(n=10),postburn sepsis group (n=20) and DAHP treatment group (n=16).In the scald control gro... | Li Hongyun Yao Yongming Shi ZhiGuo Dong Ning Yu Yan Lu Lianrong Sheng Zhiyong (C.Y.Sheng) Research Department of Burn Institute,304th Hospital of PLA,Beijing 100037 | 2002 | 感染.炎症.修复2002，3，1： | 6 |
| 4 | Reactive mesoporous silica nanoparticles loaded with limonene for improving physical and mental health of mice at simulated microgravity condition显示文摘Astronauts are under high stress for a long time because of the microgravity condition,which leads to anxiety,affects their learning and memory abilities,and seriously impairs the health of astronauts.Aromatherapy can improve the physical and mental health of astronauts in a way that moisturizes them softly and silently.However,the strong volatility of fragrances and inconvenience of aroma treatment greatly limit their application in the field of spaceflight.In this study,reactive mesoporous silica nanoparticles were prepared to encapsulate and slowly release limonene.The limonene loaded nanoparticles were named limonene@mesoporous silica nanoparticles-cyanuric chloride(LE@MSNs-CYC).LE@MSNs-CYC were then applied to wallpaper to improve the convenience of aromatherapy.LE@MSNs-CYC could chemically react with the wallpaper,thus firmly adsorbed on the wallpaper.In the following,the mice were treated with hindlimb unloading(HU)to simulate a microgravity environment.The results showed that 28-day HU led to an increase in the level of anxiety and declines in learning,memory,and physical health in mice.LE@MSNs-CYC showed significant relief effects on anxiety,learning,memory,and physical health of HU treated mice.Subsequently,the molecular mechanisms were explored by hypothalamic-pituitary-adrenal axis related hormones,immune-related cytokines,learning,and memory-related neurotransmitters and proteins. | Zhiguo Lu Jianze Wang Lina Qu Guanghan Kan Tianlu Zhang Jie Shen Yan Li Jun Yang Yunwei Niu Zuobing Xiao Yinghui Li Xin Zhang | 2020 | Bioactive Materials2020，5，4： | 5 |
| 5 | Bevacizumab biosimilar LY01008 compared with bevacizumab(Avastin)as first-line treatment for Chinese patients with unresectable,metastatic,or recurrent non-squamous non-small-cell lung cancer:A multicenter,randomized,double-blinded,phase Ⅲ trial显示文摘Background:Previous studies have demonstrated the preclinical pharmacological and toxicological consistency,and clinical pharmacokinetic equivalence of bevacizumab biosimilar LY01008 with reference bevacizumab(Avastin).This randomized controlled trial aimed to compare the efficacy and safety of LY01008 with Avastin in first-line treatment of Chinese patients with advanced or recurrent non-squamous non-small cell lung cancer(NSCLC).Methods:StageⅢB-ⅣNSCLC patients with evaluable lesions,good physical status,and adequate organ functions from 67 centers across China were randomized in a ratio of 1:1 to receive LY01008 or Avastin 15 mg/kg intravenously in combination with paclitaxel/carboplatin(combined treatment)for 4-6 cycles,followed by maintenance monotherapy with LY01008 until disease progression,intolerable toxicity,or death.The primary endpoint was objective response rate(ORR)in accordance with Response Evaluation Criteria in Solid Tumors(RECIST)version 1.1 confirmed by independent radiological review committees(IRRC).Secondary endpoints included disease control rate(DCR),duration of response(DoR),progression-free survival(PFS),overall survival(OS),and safety.This study was registered in Clinical Trials.gov(NCT03533127).Results:Between December 15^(th),2017,and May 15^(th),2019,a total of 649 patients were randomized to the LY01008(n=324)or Avastin(n=325)group.As of September 25th,2019 for primary endpoint analysis,589 patients received ORR evaluation,with a median number of combined treatment cycles of 5(range 1-6)andmedian duration of treatment of 3.0(range 0.0-5.1)months.ORRof responseevaluable patients in the LY01008 and Avastin groups were 48.5% and 53.0%,respectively.The stratified ORR ratio was 0.91(90%CI 0.80-1.04,within the prespecified equivalence margin of 0.75-1.33).Up to May 15^(th),2020,with a median follow-up of 13.6(range 0.8-28.4)months,no notable differences in DCR,median DoR,median PFS,median OS,and 1-year OS rate were observed between the LY01008 and Avastin groups.There were no clinically meaningful differences in safety and immunogenicity across treatment groups.Conclusions:LY01008 demonstrated similarity to Avastin in terms of efficacy and safety in Chinese patients with advanced or recurrent non-squamous NSCLC.LY01008 combined with paclitaxel/carboplatin is expected to become a new treatment option for unresectable,metastatic,LY01008 and Avastin groups.There were no clinically meaningful differences in safety and immunogenicity across treatment groups.Conclusions:LY01008 demonstrated similarity to Avastin in terms of efficacy and safety in Chinese patients with advanced or recurrent non-squamous NSCLC.LY01008 combined with paclitaxel/carboplatin is expected to become a new treatment option for unresectable,metastatic,or recurrent non-squamous NSCLC patients in the first-line setting. | Yuankai Shi Kaijian Lei Yuming Jia Bingqiang Ni Zhiyong He Minghong Bi Xicheng Wang Jianhua Shi Ming Zhou Qian Sun Guolei Wang Dongji Chen Yongqian Shu Lianke Liu Zhongliang Guo Yong Liu Junquan Yang Ke Wang Ke Xiao LinWu Tienan Yi Debin Sun Mafei Kang Tianjiang Ma Yimin Mao Jinsheng Shi Tiegang Tang Yan Wang Puyuan Xing Dongqing Lv Wangjun Liao Zhiguo Luo Bin Wang Xiaohong Wu Xiaoli Zhu Shuhua Han Qisen Guo Rongyu Liu Zhiwei Lu Jianyong Zhang Jian Fang Changlu Hu Yinghua Ji Guolong Liu Hong Lu Dedong Wu Junhong Zhang Shuyang Zhu Zheng Liu Wensheng Qiu Feng Ye Yan Yu Yanqiu Zhao Qinhong Zheng Jun Chen Zhanyu Pan Yiping Zhang Wenjuan Lian Bo Jiang Bo Qiu Guojun Zhang Hua Zhang Yanju Chen Yuan Chen Hongbing Duan Manxiang Li Shengming Liu Lijun Ma Hongming Pan Xia Yuan Xueli Yuan Yulong Zheng Emei Gao Li Zhao Shumin Wang Can Wu | 2021 | Cancer Communications2021，41，9： | 5 |
| 6 | Derivation of Cloud-Free-Region Atmospheric Motion Vectors from FY-2E Thermal Infrared Imagery显示文摘The operational cloud-motion tracking technique fails to retrieve atmospheric motion vectors(AMVs) in areas lacking cloud; and while water vapor shown in water vapor imagery can be used, the heights assigned to the retrieved AMVs are mostly in the upper troposphere. As the noise-equivalent temperature difference(NEd T) performance of FY-2E split window(10.3–11.5 μm, 11.6–12.8 μm) channels has been improved, the weak signals representing the spatial texture of water vapor and aerosols in cloud-free areas can be strengthened with algorithms based on the difference principle, and applied in calculating AMVs in the lower troposphere. This paper is a preliminary summary for this purpose, in which the principles and algorithm schemes for the temporal difference, split window difference and second-order difference(SD) methods are introduced. Results from simulation and cases experiments are reported in order to verify and evaluate the methods, based on comparison among retrievals and the 'truth'. The results show that all three algorithms, though not perfect in some cases,generally work well. Moreover, the SD method appears to be the best in suppressing the surface temperature influence and clarifying the spatial texture of water vapor and aerosols. The accuracy with respect to NCEP 800 h Pa reanalysis data was found to be acceptable, as compared with the accuracy of the cloud motion vectors. | Zhenhui WANG Xinxiu SUI Qing ZHANG Lu YANG Hang ZHAO Min TANG Yizhe ZHAN Zhiguo ZHANG | 2017 | Advances in Atmospheric Sciences2017，34，2： | 4 |
| 7 | Lithofacies distribution characteristics and its controlling factors of shale in Wufeng Formation-Member 1 of Longmaxi Formation in the Jiaoshiba area显示文摘It is essential to investigate shale lithofacies distribution and controlling factor of the shale for geological evaluation of shale gas exploration and development.Through comprehensive analysis of cores,thin sections,cathode luminescence,whole-rock X-ray diffraction,element capture spectroscopy,major/trace element and other data,three major types and eight sub-type shale lithofacies in the shale of Wufeng Formation-Member 1 of Longmaxi Formation in Jiaoshiba area are identified by the three-end-member method and shale lithological classification nomenclature,and the spatiotemporal distribution law and main development controlling factors of shale lithofacies are well studied.In the Jiaoshiba area,vertically,the marine shale develops siliceous shale,mixed shale and argillaceous shale from bottom to top.Besides,lateral distribution of the shale is different from north to south;the shale lithofacies in the north area changes rapidly,the mixed shale in the north area is much thicker than that in the south area,while the siliceous shale in the south area is relatively thicker.Difference in the shale lithofacies is controlled by special sedimentary geologic events;development of the siliceous shale is controlled by the Ordovician-Silurian global volcanic event to some extent,while the mixed shale is significantly influenced by effect of bottom current,and the argillaceous shale is mainly affected by supply of terrestrial clastic material. | Chao Wang Baiqiao Zhang Yongchao Lu Zhiguo Shu Yaqiu Lu Hanyong Bao Zhiyong Meng Lei Chen | 2018 | Petroleum Research2018，3，4： | 4 |
| 8 | Influence of Temperature and Frequency on Microwave Dielectric Properties of Lunar Regolith Simulant显示文摘The dielectric constant of the lunar regolith can directly influence the reflection coefficient and the trans-mission coefficient of the Moon′s surface, and plays an important role in the Moon research. In order to study the di-electric properties of the lunar regolith, the lunar regolith simulant was made according to the making procedure of the CAS-1 simulant made by Chinese Academy of Sciences. Then the dielectric constants of the lunar regolith simulant were measured with 85070E Aiglent Microwave Network Analyzer in the frequency ranging from 0.2 GHz to 20.0 GHz and at temperature of 25.1℃, 17.7℃, 13.1℃, 11.5℃, 9.6℃, 8.0℃, 4.1℃, -0.3℃, -4.7℃, -9.5℃, -18.7℃, -27.7℃, and -32.6℃, respectively. The Odelevsky model was employed to remove the influence of water in the air on the final effective dielectric constants. The results indicate that frequency and temperature have apparent influences on the dielectric constants of the lunar regolith simulant. The real parts of the dielectric constants increase fast over the range of 0.2 GHz to 3.0 GHz, but decrease slowly over the range of 4.0 GHz to 20.0 GHz. The opposite phenomenon occurs in the imaginary parts. The influences of the frequency and temperature on the brightness temperature were also estimated based on the radiative transfer equation. The result shows that the variation of the frequency and temperature results in great changes of the microwave brightness temperature emitting from the lunar regolith. | MENG Zhiguo CHEN Shengbo DU Xiaojuan EDWARD Matthew Osei Jnr LU Peng WANG Zijun | 2011 | Chinese Geographical Science2011，21，1： | 3 |
| 9 | Dynamic failure risk of coal pillar formed by irregular shape longwall face:A case study显示文摘Irregular shape workface would result in the presence of coal pillar, which leads to high stress concentration and possibly induces coal bumps. In order to study the coal bump mechanism of pillars, static and dynamic stress overlapping(SDSO) method was proposed to explain the impacts of static stress concentration and tremors induced by mining activities. The stress and deformation in surrounding rock of mining face were analyzed based on the field case study at 1303 workface in Zhaolou Coal Mine in China.The results illustrate that the surrounding rock of a workface could be divided into four different zones,i.e., residual stress zone, stress decrease zone, stress increase zone and original stress zone. The stress increase zone is prone to failure under the SDSO impact loading conditions and will provide elastic energy for inducing coal bump. Based on the numerical modelling results, the evolution of static stress in coal pillar as the size of gob increasing was studied, and the impact of dynamic stress was investigated through analyzing the characteristics of tremor activities. The numerical results demonstrate the peak value of vertical stress in coal pillar rises from about 30 MPa with mining distance 10 m to 52.6 MPa with mining distance 120 m, and the location of peak stress transfers to the inner zone of coal pillars as the workface moves forward. For the daily tremor activities, tremors with high energy released indicate high dynamic stress disturbance on the surrounding rock, therefore, the impact of dynamic stressing is more serious during workface extension period because the tremor frequency and average energy after workface extension are higher than those before the workface extension. | Yixin Zhao Hao Wang Shimin Liu Zonglong Mu Zhiguo Lu | 2018 | International Journal of Mining Science and Technology2018，28，5： | 3 |
| 10 | Review on retrieval of lunar regolith thickness by active and passive microwave measurements显示文摘It is one of the important methods to retrieve lunar regolith thickness using active and passive microwave techniques.The retrieval of lunar regolith thickness is based on microwave radiation transfer process simulation in the regolith media.The lunar regolith model is first introduced,and the features of the involved physical parameters are indicated thereafter,such as dielectric constants,surface roughness,particle size and thermal grads of the lunar regolith.The time delay and the migration of the radar echoes from the different interfaces is the key problem for active microwave measurement.And the simulation of the microwave radiative transfer in the regolith media is the important technique for the passive microwave measurement.The important parameters and the physical mechanism for the two measurements are also presented. | Zhiguo MENG Shengbo CHEN Cai LIU Xiaojuan DU Tao MENG Zijun WANG Hang LU | 2008 | Global Geology2008，11，2： | 3 |
| 11 | The design and construction of a pulsed beam generation system based on high intensity cyclotron显示文摘In order to perform the studies on a pulsed beam generation system based on a high intensity cyclotron, a test beam line with a pulsed beam generation for a 10 MeV compact cyclotron (CYCIAE-10) has been designed and constructed at China Institute of Atomic Energy (CIAE). A 70 MHz continuous H- beam can be pulsed to the pulse length of less than 10 ns with a repetition rate of 4.4 MHz. The sine waveform with a frequency of 2.2 MHz is adopted for the chopper and a mesh structure with single drift and dual gaps is used for the 70 MHz buncher. A helical resonator is designed and constructed based on simulations and experiments on the RF matching for the chopper. A helical inductance loop that is exceptionally large of its kind and equipped with water cooling for the resonator has been successfully wound and a 500 W solid RF amplifier has been manufactured. A special measuring device has been designed, which can be used to measure both the DC beam and the pulsed beam. The required pulsed beam was obtained after pulsed beam tuning. | AN ShiZhong YIN ZhiGuo LI PengZhan SONG GuoFang WU LongCheng GUAN FengPing XIE HuaiDong JIA XianLu LU YinLong ZHANG TianJue | 2011 | Science China(Physics,Mechanics & Astronomy)2011，54，S2： | 3 |
| 12 | Comprehensive test stand for high-intensity cyclotron development显示文摘A comprehensive test stand for high-intensity cyclotron development,a 10 MeV 430 μA cyclotron,has been successfully constructed at China Institute of Atomic Energy (CIAE) for the experimental verification of the viability of a 100 MeV high-intensity cyclotron (CYCIAE-100) being constructed at CIAE.The verification includes the overall design technology,detail design and engineering technology for the crucial parts of the machine,e.g.main magnet and beam diagnostics.It will also pave the way for future enhancement of the beam intensity.The test stand consists of a H-ion source,axial injection system,central region,main magnet and coil,RF resonator,stripping extraction,and auxiliary systems,which include the RF power amplifier,high voltage and DC power supplies,electric apparatus,beam diagnostics,control,vacuum,water cooling,pneumatic system,and safety interlock.This paper presents the work regarding the design and sub-system development of the test sand,and the results of the beam commissioning of the machine. | ZHANG TianJue LI ZhenGuo CHU ChengJie XING JianSheng GUAN FengPing ZHONG JunQing JI Bin GE Tao YIN ZhiGuo HOU ShiGang PAN GaoFeng YAO HongJuan LU YinLong WANG ZhenHui WU LongCheng LIN Jun JIA XianLu WEI SuMin WEN LiPeng WANG Feng XIA Le CAI HongRu XIE HuaiDong CHEN RongFan ZHANG Yan ZHANG SuPing LIU GengShou ZOU Jian AN ShiZhong YANG JianJun BI YuanJie YANG Fang | 2011 | Chinese Science Bulletin2011，56，3： | 3 |
| 13 | High-Throughput Rice Density Estimation from Transplantation to Tillering Stages Using Deep Networks显示文摘Rice density is closely related to yield estimation,growth diagnosis,cultivated area statistics,and management and damage evaluation.Currently,rice density estimation heavily relies on manual sampling and counting,which is inefficient and inaccurate.With the prevalence of digital imagery,computer vision(CV)technology emerges as a promising alternative to automate this task.However,challenges of an in-field environment,such as illumination,scale,and appearance variations,render gaps for deploying CV methods.To fill these gaps towards accurate rice density estimation,we propose a deep learningbased approach called the Scale-Fusion Counting Classification Network(SFC^(2)Net)that integrates several state-of-the-art computer vision ideas.In particular,SFC^(2)Net addresses appearance and illumination changes by employing a multicolumn pretrained network and multilayer feature fusion to enhance feature representation.To ameliorate sample imbalance engendered by scale,SFC^(2)Net follows a recent blockwise classification idea.We validate SFC^(2)Net on a new rice plant counting(RPC)dataset collected from two field sites in China from 2010 to 2013.Experimental results show that SFC^(2)Net achieves highly accurate counting performance on the RPC dataset with a mean absolute error(MAE)of 25.51,a root mean square error(MSE)of 38.06,a relative MAE of 3.82%,and a R^(2) of 0.98,which exhibits a relative improvement of 48.2%w.r.t.MAE over the conventional counting approach CSRNet.Further,SFC^(2)Net provides high-throughput processing capability,with 16.7 frames per second on 1024×1024 images.Our results suggest that manual rice counting can be safely replaced by SFC^(2)Net at early growth stages.Code and models are available online at https://git.io/sfc2net. | Liang Liu Hao Lu Yanan Li Zhiguo Cao | 2020 | Plant Phenomics2020，2，1： | 2 |
| 14 | Incidence, risk factors for amputation among patients with diabetic foot ulcer in a Chinese tertiary hospital显示文摘 | Xiang Li Ting Xiao Yuzhen Wang Hongbin Gu Zhiguo Liu Yufeng Jiang Yanjun Liu Zuqian Lu Xiaopin Yang Yin Lan Zhangrong Xu | 2011 | Diabetes Research and Clinical Practice2011，，1： | 2 |
| 15 | Synthesis and characterization of novel biocompatible nanocapsules encapsulated lily fragrance显示文摘Fragranced products are wildly used in our daily life. However, their storage, application and quality are severely influenced by their strong volatility. In order to overcome these natural defects of fragrances,fragrance encapsulation approaches have been developed recently. In this study, a mild, simple and lowcost method of fragrance encapsulation was invented. Liposomes composed of soya lecithin/DSPEPEG2000/PLGA were prepared to encapsulate lily fragrance(LF). And these nanosized fragrance products was named LF-NPs. The hydrodynamic diameters of LF-NPs were approximately 100 nm and their encapsulation efficiency was up to 21.9%. Besides, LF-NPs could also prolong the release time of lily fragrance. Finally, the cytotoxicity tests indicated that LF-NPs offered biocompatibility. Therefore, LF-NPs were expected to have application prospects. | Weihong Ji Tianlu Zhang Zhiguo Lu Jie Shen Zuobing Xiao Xin Zhang | 2019 | Chinese Chemical Letters2019，30，3： | 2 |
| 16 | Motor neuron replacement therapy for amyotrophic lateral sclerosis显示文摘Amyotrophic lateral sclerosis is a motor neuron degenerative disease that is also known as Lou Gehrig’s disease in the United States,Charcot’s disease in France,and motor neuron disease in the UK.The loss of motor neurons causes muscle wasting,paralysis,and eventually death,which is commonly related to respiratory failure,within 3-5 years after onset of the disease.Although there are a limited number of drugs approved for amyotrophic lateral sclerosis,they have had little success at treating the associated symptoms,and they cannot reverse the course of motor neuron degeneration.Thus,there is still a lack of effective treatment for this debilitating neurodegenerative disorder.Stem cell therapy for amyotrophic lateral sclerosis is a very attractive strategy for both basic and clinical researchers,particularly as transplanted stem cells and stem cell-derived neural progenitor/precursor cells can protect endogenous motor neurons and directly replace the lost or dying motor neurons.Stem cell therapies may also be able to re-establish the motor control of voluntary muscles.Here,we review the recent progress in the use of neural stem cells and neural progenitor cells for the treatment of amyotrophic lateral sclerosis.We focus on MN progenitor cells derived from fetal central nervous system tissue,embryonic stem cells,and induced pluripotent stem cells.In our recent studies,we found that transplanted human induced pluripotent stem cell-derived motor neuron progenitors survive well,differentiate into motor neurons,and extend axons into the host white matter,not only in the rostrocaudal direction,but also along motor axon tracts towards the ventral roots in the immunodeficient rat spinal cord.Furthermore,the significant motor axonal extension after neural progenitor cell transplantation in amyotrophic lateral sclerosis models demonstrates that motor neuron replacement therapy could be a promising therapeutic strategy for amyotrophic lateral sclerosis,particularly as a variety of stem cell derivatives,including induced pluripotent stem cells,are being considered for clinical trials for various diseases. | Bochao Liu Mo Li Lingyan Zhang Zhiguo Chen Paul Lu | 2022 | Neural Regeneration Research2022，17，8： | 2 |
| 17 | Disuccinimidyl suberate cross-linked hemoglobin as a novel red blood cell substitute显示文摘Disuccinimidyl suberate (DSS) intramolecularly cross-linked hemoglobin (Hb) was developed as a novel red blood cell substitute. A multi-angle laser light scattering detector coupled with size exclusion HPLC was applied to determine the molecular weight of the modified Hb. SDS-PAGE was also used as a complement. It was proved that 83.8% of the product was intramolecularly cross-linked Hb with weight-average molecular weights (Mw) of 67.5 kD, 12% was dimeric Hb with Mw of 146.6 kD, and 4.2% was trimeric Hb with Mw of 306.4 kD. The tetramer structure of the cross-linked Hb was stable as shown in size-exclusion chromatography using a mobile phase containing 1 mol/L MgCl2. Analysis by LC-MS demonstrated that the reaction of DSS with Hb mainly took place between the twoα subunits within a Hb molecule, resulting in stabilization of the tetramer structure. However, the cross-linking was not site-specific. The P50 of the cross-linked Hb decreased from 21.8 mmHg to 14.3 mmHg, and the Hill coefficient decreased from 2.22 to 1.41. Result of isoelectric focusing showed that the pI of DSS cross-linked Hb was in the range of 4.6-5.2, similar to that of serum albumin. The safety of DSS cross-linked Hb was favored by animal tests on rats and guinea pigs. Exchange transfusion experiment with DSS cross-linked Hb using rats as a model indicated no pressor effect or other significant side effects. The characteristics and properties of DSS cross-linked Hb were also compared with that of diaspirin cross-linked Hb reported in the literature. | LU Xiuling, ZHENG Chunyang, XU Yuhong & SU Zhiguo National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100080, China School of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China | 2005 | Science China(Life Sciences)2005，48，1： | 1 |
| 18 | The application of a new structural control concept for tall building with large podium structure显示文摘 | Xilin Lu Zhiguo Gong Dagen Weng | 2007 | Engineering Structures2007，29，： | 1 |
| 19 | The dike stability analysis based on strength reduction method显示文摘 | Niu Zhiguo You Ri Lu Jun | 2012 | International Conference onModem Hydraulic Engineering2012，28，： | 1 |
| 20 | Endogenous metabolism of Candidatus Accumulibacter phosphatis under various starvation conditions 显示文摘 | LU Huabing KELLER J YUAN Zhiguo | 2007 | Water Research2007，41，： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员